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<p><span>Polyclonal antibody raised in rabbit against human PAPD4 (PAP associated domain containing 4), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminal part of the protein.</span></p>',
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<p><small><strong>Figure 1. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PAPD4 (Cat. No. CS-130-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:107,000.</small></p>
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<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310130_fig2.jpg" alt="Western blot" height="129" width="119" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PAPD4</strong><br />Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against PAPD4 (Cat. No. CS-130-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 56 kDa); the marker (in kDa) is shown on the left.</small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PAPD4</strong><br />Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against PAPD4 (Cat. No. CS-130-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 56 kDa); the marker (in kDa) is shown on the left.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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<p><small><strong>Figure 1. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PAPD4 (Cat. No. CS-130-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:107,000.</small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PAPD4</strong><br />Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against PAPD4 (Cat. No. CS-130-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 56 kDa); the marker (in kDa) is shown on the left.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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<p><span>Polyclonal antibody raised in rabbit against human PAPD4 (PAP associated domain containing 4), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminal part of the protein.</span></p>',
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<p><small><strong>Figure 1. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PAPD4 (Cat. No. CS-130-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:107,000.</small></p>
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<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15310130_fig2.jpg" alt="Western blot" height="129" width="119" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PAPD4</strong><br />Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against PAPD4 (Cat. No. CS-130-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 56 kDa); the marker (in kDa) is shown on the left.</small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PAPD4</strong><br />Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against PAPD4 (Cat. No. CS-130-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 56 kDa); the marker (in kDa) is shown on the left.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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<p><small><strong>Figure 1. Determination of the titer</strong><br />To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PAPD4 (Cat. No. CS-130-100). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:107,000.</small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PAPD4</strong><br />Nuclear extracts of HeLa cells (40 μg) were analysed by Western blot using the Diagenode antibody against PAPD4 (Cat. No. CS-130-100) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 56 kDa); the marker (in kDa) is shown on the left.</small></p>
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'created' => '2015-07-03 16:05:27',
'ProductsDocument' => array(
'id' => '1657',
'product_id' => '2118',
'document_id' => '38'
)
)
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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