ELISA Enzyme-linked immunosorbent assay. Read more |
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Lot | A1024-001 |
---|---|
Concentration | not determined |
Species reactivity | Mouse |
Type | Polyclonal |
Purity | Whole antiserum |
Host | Rabbit |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Applications | Suggested dilution | References |
---|---|---|
ELISA | 1:500 | Fig 1 |
* The optimal dilution for other applications should be determined by the end user. For WB we suggest starting with a 1:1,000 dilution.
Figure 1. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against mouse PHC2 (cat. No. CS-PA020-100). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:33,300.
Datasheet PHC2 CS-PA020-100 DATASHEET Datasheet description | Download |
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
How to properly cite this product in your workDiagenode strongly recommends using this: PHC2 polyclonal antibody (Diagenode Cat# C15310240 Lot# A1024-001). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'PHC2 polyclonal antibody - Pioneer', 'meta_title' => 'PHC2 polyclonal antibody - Pioneer', 'product' => array( 'Product' => array( 'id' => '2152', 'antibody_id' => '276', 'name' => 'PHC2 polyclonal antibody ', 'description' => '<p><span>Alternative names: <strong>EDR2</strong>, <strong>HPH2</strong>, <strong>PH2</strong></span></p> <p><span>Polyclonal antibody raised in rabbit against mouse PHC2 (polyhomeotic homolog 2) using two KLH-conjugated synthetic peptides containing a sequence from the central region of the protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15310240-Fig1-ELISA.jpg" alt="ELISA" height="334" width="400" /></p> </div> <div class="small-8 columns"> <p><small><strong>Figure 1. 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