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PML polyclonal antibody

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Catalog Number

Format

Price

C15410108
(pAb-108-050)

50 µg/360 µl

$260.00
Bulk order

Alternative names: MYL, PP8675, RNF71, TRIM19

Polyclonal antibody raised in rabbit against human PML (Promyelocytic leukemia), using 3 different KLH-conjugated synthetic peptides.

Lot	A136-0011
Concentration	0.14 µg/µl
Species reactivity	Human
Type	Polyclonal
Purity	Affinity purified
Host	Rabbit
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ELISA	1:100 - 1:200	Fig 1
Western Blotting	1:750	Fig 2

Validation Data

Figure 1. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PML (Cat. No. pAb-108-050), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:6,000.

Figure 2. Western blot analysis using the Diagenode antibody directed against PML
Human embryonic kidney cells (293T) were transfected with an ER-tagged PML/RAR fusion protein. Nuclear extracts (50 μg) from control (lane 1) and from transfected cells (lane 2) were analysed by Western blot using the Diagenode antibody against PML (Cat. No. pAb-108-050). The antibody was diluted 1:750 in TBS-Tween containing 5% milk powder. The position of the PML/RAR fusion protein is indicated on the right; the marker (in kDa) is shown on the left. The smaller band of approximately 60 kDa might represent the endogenous PML protein.

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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
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<p>Polyclonal antibody raised in rabbit against human PML (Promyelocytic leukemia), using 3 different KLH-conjugated synthetic peptides.</p>',
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<div class="small-5 columns">
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<p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PML (Cat. No. pAb-108-050), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:6,000.</small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PML</strong><br />Human embryonic kidney cells (293T) were transfected with an ER-tagged PML/RAR fusion protein. Nuclear extracts (50 &mu;g) from control (lane 1) and from transfected cells (lane 2) were analysed by Western blot using the Diagenode antibody against PML (Cat. No. pAb-108-050). The antibody was diluted 1:750 in TBS-Tween containing 5% milk powder. The position of the PML/RAR fusion protein is indicated on the right; the marker (in kDa) is shown on the left. The smaller band of approximately 60 kDa might represent the endogenous PML protein.</small></p>
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<div class="small-5 columns">
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<p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PML (Cat. No. pAb-108-050), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:6,000.</small></p>
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<div class="row">
<div class="small-5 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410108_fig2.jpg" alt="Western blot" height="175" width="178" /></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PML</strong><br />Human embryonic kidney cells (293T) were transfected with an ER-tagged PML/RAR fusion protein. Nuclear extracts (50 &mu;g) from control (lane 1) and from transfected cells (lane 2) were analysed by Western blot using the Diagenode antibody against PML (Cat. No. pAb-108-050). The antibody was diluted 1:750 in TBS-Tween containing 5% milk powder. The position of the PML/RAR fusion protein is indicated on the right; the marker (in kDa) is shown on the left. The smaller band of approximately 60 kDa might represent the endogenous PML protein.</small></p>
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<td>Fig 1</td>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Expert technical support</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Sample sizes available</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 100% satisfaction guarantee</span></li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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			'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
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	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
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			'antibody_id' => '317',
			'name' => 'PML polyclonal antibody ',
			'description' => '<p>Alternative names: <strong>MYL</strong>, <strong>PP8675</strong>, <strong>RNF71</strong>, <strong>TRIM19</strong></p>
<p>Polyclonal antibody raised in rabbit against human PML (Promyelocytic leukemia), using 3 different KLH-conjugated synthetic peptides.</p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410108_fig1.jpg" alt="ELISA" height="295" width="343" /></p>
</div>
<div class="small-7 columns">
<p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PML (Cat. No. pAb-108-050), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:6,000.</small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410108_fig2.jpg" alt="Western blot" height="175" width="178" /></p>
</div>
<div class="small-7 columns">
<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PML</strong><br />Human embryonic kidney cells (293T) were transfected with an ER-tagged PML/RAR fusion protein. Nuclear extracts (50 &mu;g) from control (lane 1) and from transfected cells (lane 2) were analysed by Western blot using the Diagenode antibody against PML (Cat. No. pAb-108-050). The antibody was diluted 1:750 in TBS-Tween containing 5% milk powder. The position of the PML/RAR fusion protein is indicated on the right; the marker (in kDa) is shown on the left. The smaller band of approximately 60 kDa might represent the endogenous PML protein.</small></p>
</div>
</div>',
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			'format' => '50 µg/360 µl',
			'catalog_number' => 'C15410108',
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			'sf_code' => 'C15410108-D001-000581',
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			'search_order' => '03-Antibody',
			'price_EUR' => '260',
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			'price_JPY' => '40730',
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			'meta_title' => 'PML polyclonal antibody - Pioneer',
			'meta_keywords' => '',
			'meta_description' => 'PML polyclonal antibody - Pioneer',
			'modified' => '2022-01-05 15:55:57',
			'created' => '2015-06-29 14:08:20',
			'locale' => 'jpn'
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		'Antibody' => array(
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			'id' => '317',
			'name' => 'PML polyclonal antibody',
			'description' => 'PML (UniProtKB/Swiss-Prot entry P29590) is a transcription factor and tumor supressor which belongs to the tripartite motif (TRIM) family. This motif contains three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. PML regulates the response of p53 to oncogenic signals. It is often involved in a chromosomal translocation between chromosomes 15 and 17 which results in a PML/RARalpha fusion protein and is associated with most cases of acute promyelocytic leukemia.',
			'clonality' => '',
			'isotype' => '',
			'lot' => 'A136-0011 ',
			'concentration' => '0.14 &micro;g/&micro;l',
			'reactivity' => 'Human',
			'type' => 'Polyclonal',
			'purity' => 'Affinity purified',
			'classification' => 'Pioneer',
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<thead>
<tr>
<th>Applications</th>
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<p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PML (Cat. No. pAb-108-050), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:6,000.</small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PML</strong><br />Human embryonic kidney cells (293T) were transfected with an ER-tagged PML/RAR fusion protein. Nuclear extracts (50 &mu;g) from control (lane 1) and from transfected cells (lane 2) were analysed by Western blot using the Diagenode antibody against PML (Cat. No. pAb-108-050). The antibody was diluted 1:750 in TBS-Tween containing 5% milk powder. The position of the PML/RAR fusion protein is indicated on the right; the marker (in kDa) is shown on the left. The smaller band of approximately 60 kDa might represent the endogenous PML protein.</small></p>
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
$name = 'WB'
$document = array(
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	'name' => 'Epigenetic Antibodies Brochure',
	'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. Since 10 years, Diagenode has developed the most stringent quality production available on the market for antibodies exclusively focused on epigenetic uses. All our antibodies have been qualified to work in epigenetic applications.</p>',
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	'language' => 'es',
	'url' => 'files/SDS/PML/SDS-C15410108-PML_Antibody-ES-es-GHS_1_0.pdf',
	'countries' => 'ES',
	'modified' => '2023-01-26 09:49:22',
	'created' => '2023-01-26 09:49:22',
	'ProductsSafetySheet' => array(
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
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$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
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	'meta_keywords' => '',
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<p>Polyclonal antibody raised in rabbit against human PML (Promyelocytic leukemia), using 3 different KLH-conjugated synthetic peptides.</p>',
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			'info1' => '<div class="row">
<div class="small-5 columns">
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<p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PML (Cat. No. pAb-108-050), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:6,000.</small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410108_fig2.jpg" alt="Western blot" height="175" width="178" /></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PML</strong><br />Human embryonic kidney cells (293T) were transfected with an ER-tagged PML/RAR fusion protein. Nuclear extracts (50 &mu;g) from control (lane 1) and from transfected cells (lane 2) were analysed by Western blot using the Diagenode antibody against PML (Cat. No. pAb-108-050). The antibody was diluted 1:750 in TBS-Tween containing 5% milk powder. The position of the PML/RAR fusion protein is indicated on the right; the marker (in kDa) is shown on the left. The smaller band of approximately 60 kDa might represent the endogenous PML protein.</small></p>
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<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
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<tr>
<td>ELISA</td>
<td>1:100 - 1:200</td>
<td>Fig 1</td>
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<tr>
<td>Western Blotting</td>
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<td>Fig 2</td>
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		'name' => 'PML polyclonal antibody ',
		'description' => '<p>Alternative names: <strong>MYL</strong>, <strong>PP8675</strong>, <strong>RNF71</strong>, <strong>TRIM19</strong></p>
<p>Polyclonal antibody raised in rabbit against human PML (Promyelocytic leukemia), using 3 different KLH-conjugated synthetic peptides.</p>',
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		'info1' => '<div class="row">
<div class="small-5 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410108_fig1.jpg" alt="ELISA" height="295" width="343" /></p>
</div>
<div class="small-7 columns">
<p><small><strong>Figure 1. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PML (Cat. No. pAb-108-050), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:6,000.</small></p>
</div>
</div>
<div class="row">
<div class="small-5 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410108_fig2.jpg" alt="Western blot" height="175" width="178" /></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PML</strong><br />Human embryonic kidney cells (293T) were transfected with an ER-tagged PML/RAR fusion protein. Nuclear extracts (50 &mu;g) from control (lane 1) and from transfected cells (lane 2) were analysed by Western blot using the Diagenode antibody against PML (Cat. No. pAb-108-050). The antibody was diluted 1:750 in TBS-Tween containing 5% milk powder. The position of the PML/RAR fusion protein is indicated on the right; the marker (in kDa) is shown on the left. The smaller band of approximately 60 kDa might represent the endogenous PML protein.</small></p>
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<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
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<tr>
<td>ELISA</td>
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<td>Fig 1</td>
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<tr>
<td>Western Blotting</td>
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<td>Fig 2</td>
</tr>
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<div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div>
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			'tabular' => true,
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			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Expert technical support</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Sample sizes available</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 100% satisfaction guarantee</span></li>
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			'name' => 'All antibodies',
			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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Datasheet PML pAb-108-05 DATASHEET Datasheet description	Download
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download

PML Antibody SDS GB en	Download
PML Antibody SDS US en	Download
PML Antibody SDS DE de	Download
PML Antibody SDS JP ja	Download
PML Antibody SDS BE nl	Download
PML Antibody SDS FR fr	Download
PML Antibody SDS BE fr	Download
PML Antibody SDS ES es	Download

PML polyclonal antibody

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