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<p><span>Polyclonal antibody raised in rabbit against human PPARG (peroxisome proliferator-activated receptor gamma), using a KLH-conjugated synthetic peptide containing a sequence from the central part of the protein.</span></p>',
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<p><small><strong>Figure 1. Determination of the titer</strong><br /> To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human PPARG (Cat. No. C15310133). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:27,600.</small></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PPARG</strong><br />293T cells were analysed by Western blot using the Diagenode antibody against PPARG (Cat. No. C15310133) diluted 1:500. Figure 2 shows the result of 293T cells transfected with pNTAP-PPARG (lane 2) or with an empty vector (lane 1). The position of the protein of interest is indicated on the right (expected size: 58 kDa); the marker (in kDa) is shown on the left.</small></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
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<p><small> <strong>Figure 2. Western blot analysis using the Diagenode antibody directed against PPARG</strong><br />293T cells were analysed by Western blot using the Diagenode antibody against PPARG (Cat. No. C15310133) diluted 1:500. Figure 2 shows the result of 293T cells transfected with pNTAP-PPARG (lane 2) or with an empty vector (lane 1). The position of the protein of interest is indicated on the right (expected size: 58 kDa); the marker (in kDa) is shown on the left.</small></p>
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'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
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)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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