| Protein extraction from Tissues and Cultured Cells using Bioruptor® Standard & Plus Protein extraction from tissues and cultured cells is the first step for many biochemical and ana... | Download |
Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Diagenode’s Bioruptor® Plus used in combination with the Protein Extraction kit ensures efficient disruption, homogenization and high yields of proteins from tissues. Tubes are already pre-filled with the protein extraction beads which have been designed to enhance the protein extraction process.
| Protein extraction from Tissues and Cultured Cells using Bioruptor® Standard & Plus Protein extraction from tissues and cultured cells is the first step for many biochemical and ana... | Download |
| Protein Extraction kit manual MANUAL Protein extraction from tissues is the first step for many biochemical and analytical tec... | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: Protein Extraction kit for Bioruptor® Plus (Diagenode Cat# C20000020). Click here to copy to clipboard. Using our products in your publication? Let us know! |
The Roles of the Interaction of BCL2-Antagonist/Killer 1, Apoptotic Peptidase Activating Factor 1 and Selenium in the Pathogenesis of Kashin-Beck Disease |
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An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> </div> </div> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => 'View accessories & consumables for Bioruptor<sup>®</sup> Plus', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; 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', 'meta_description' => 'Diagenode offers Cell lysis & Tissue Homogenizationa for use with the Bioruptor® Sonication Devices.', 'meta_title' => 'Cell lysis and Tissue Homogenization using Bioruptor® Sonication Devices | Diagenode', 'modified' => '2019-07-03 11:04:38', 'created' => '2015-02-07 19:59:35', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '94', 'name' => 'Protein Extraction kit manual', 'description' => '<div class="page" title="Page 4"> <div class="section"> <div class="layoutArea"> <div class="column"> <p><span>Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Efficient disruption and homogenization of animal tissues are required to ensure high yields of proteins. Diagenode’s Bioruptor</span><sup><span>® </span></sup><span>uses state-of- the-art ultrasound technology to efficiently disrupt and homogenize tissues in just one step. </span></p> </div> </div> </div> </div>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/kits/Protein_Extraction_kit_manual.pdf', 'slug' => 'protein-extraction-kit-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-07-24 17:22:14', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1776', 'name' => 'product/kits/kit-icon.png', 'alt' => 'Kit icon', 'modified' => '2019-04-09 12:06:37', 'created' => '2018-03-15 15:51:02', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( 'id' => '23', 'name' => 'Protein extraction from Tissues and Cultured Cells using Bioruptor® Standard & Plus', 'description' => '<p><span>Protein extraction from tissues and cultured cells is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Efficient disruption and homogenization of animal tissues and cultured cells are required to ensure high yields of proteins. Diagenode’s Bioruptor</span><span>® </span><span>uses state-of-the-art ultrasound technology to efficiently disrupt and homogenize tissues and cultured cells in just one step. </span></p>', 'image_id' => '213', 'type' => 'Protocol', 'url' => 'files/protocols/Protein_extraction_from_Tissues_and_cultured_cells_protocol_standard_Plus.pdf', 'slug' => 'protein-extraction-from-tissues-and-cultured-cells-protocol-standard-plus', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:40:14', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ) ), 'Publication' => array( (int) 0 => array( 'id' => '2862', 'name' => 'The Roles of the Interaction of BCL2-Antagonist/Killer 1, Apoptotic Peptidase Activating Factor 1 and Selenium in the Pathogenesis of Kashin-Beck Disease', 'authors' => 'Wang S, Duan C, Zhang F, Wang X, Guo X', 'description' => '<p>BCL2-antagonist/killer 1 (BAK1) and apoptotic peptidase activating factor 1 (APAF1) are significant genes in apoptosis signalling pathway of Kashin-Beck disease (KBD). We aimed to verify the protein expression levels of BAK1 and APAF1 in the cartilage and chondrocytes of patients with KBD. Additionally, we explored the relationship between the levels of these proteins and selenium concentration. Chondrocytes was cultured and treated with sodium selenite in vitro. Immunohistochemistry and Western blotting were used to verify the expression levels of BAK1 and APAF1. Compared with the control samples, APAF1 was upregulated and BAK1 was downregulated in the cartilage and chondrocytes of KBD patients. APAF1 expression was higher in the middle and deep zone in the KBD cartilage. APAF1 levels decreased gradually with the increasing selenium concentration (0.05, 0.10 and 0.25 mg/L). BAK1 expression in the 0.25 mg/L selenium group was lower than that of the control group. Different selenium concentrations had varying effects on BAK1 and APAF1 levels. APAF1 may play an important role in the pathogenesis of KBD. APAF1-related apoptosis was more pronounced in the middle and deep zones of the KBD cartilage. APAF may represent a potentially novel molecular target, which may be a biomarker of the role of selenium on the prevention and treatment of KBD. The role of BAK1 in the pathogenesis of KBD requires further study.</p>', 'date' => '2016-03-01', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26179084', 'doi' => '10.1007/s12011-015-0424-2', 'modified' => '2016-03-17 10:27:42', 'created' => '2016-03-17 10:27:42', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/bioruptor-plus-sonication-device"><img src="/img/product/shearing_technologies/bioruptor_plus.jpg" alt="Bioruptor Plus Sonication Device" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01020001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: QUOTE MODAL --><div id="quoteModal-1784" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Bioruptor<sup>®</sup> Plus sonication device</strong>. 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</div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01200016</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1796" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/1796" id="CartAdd/1796Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1796" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico', 'B01200016', '350', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico', 'B01200016', '350', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack" 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Efficient disruption and homogenization of animal tissues are required to ensure high yields of proteins. 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We aimed to verify the protein expression levels of BAK1 and APAF1 in the cartilage and chondrocytes of patients with KBD. Additionally, we explored the relationship between the levels of these proteins and selenium concentration. Chondrocytes was cultured and treated with sodium selenite in vitro. Immunohistochemistry and Western blotting were used to verify the expression levels of BAK1 and APAF1. Compared with the control samples, APAF1 was upregulated and BAK1 was downregulated in the cartilage and chondrocytes of KBD patients. APAF1 expression was higher in the middle and deep zone in the KBD cartilage. APAF1 levels decreased gradually with the increasing selenium concentration (0.05, 0.10 and 0.25 mg/L). BAK1 expression in the 0.25 mg/L selenium group was lower than that of the control group. Different selenium concentrations had varying effects on BAK1 and APAF1 levels. APAF1 may play an important role in the pathogenesis of KBD. APAF1-related apoptosis was more pronounced in the middle and deep zones of the KBD cartilage. APAF may represent a potentially novel molecular target, which may be a biomarker of the role of selenium on the prevention and treatment of KBD. 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An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> </div> </div> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => 'View accessories & consumables for Bioruptor<sup>®</sup> Plus', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'format' => '1 unit', 'catalog_number' => 'B01020001', 'old_catalog_number' => 'UCD-300 TM', 'sf_code' => 'B01020001-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '22500', 'price_USD' => '29500', 'price_GBP' => '19500', 'price_JPY' => '3524625', 'price_CNY' => '/', 'price_AUD' => '73750', 'country' => 'ALL', 'except_countries' => 'Japan', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'bioruptor-plus-sonication-device', 'meta_title' => 'Bioruptor® Plus sonication device', 'meta_keywords' => '', 'meta_description' => 'Bioruptor® Plus sonication device', 'modified' => '2024-08-22 15:29:44', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1796', 'antibody_id' => null, 'name' => '15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico', 'description' => '<p><span>This tube holder has been designed for being used in combination with the Bioruptor® Standard, Plus and Pico for tissue disruption and cell lysis. </span></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '1 pack', 'catalog_number' => 'B01200016', 'old_catalog_number' => 'O-ring-15', 'sf_code' => 'B01200016-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '300', 'price_USD' => '350', 'price_GBP' => '250', 'price_JPY' => '46995', 'price_CNY' => '', 'price_AUD' => '875', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack', 'meta_title' => '15 ml sonication accessories for Bioruptor® Standard & Plus & Pico', 'meta_keywords' => '', 'meta_description' => '15 ml sonication accessories for Bioruptor® Standard & Plus & Pico', 'modified' => '2022-01-25 04:00:28', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'Protein extraction', 'description' => '<div class="row"> <div class="large-12 columns">Various biochemical and analytical techniques require the extraction of protein from tissues or mammalian, yeast and bacterial cells. Obtaining high quality and yields of proteins is important for further downstream protein characterization such as in PAGE, western blotting, mass spectrometry or protein purification. The efficient disruption and homogenization of tissues and cultured cells obtained in just one step using <strong>Diagenode's Bioruptor</strong><sup>®</sup> deliver high quality protein.</div> </div> <p></p> <div class="row"> <div class="small-6 medium-6 large-6 columns text-center"><img src="https://www.diagenode.com/img/applications/protein_extraction_standard_plus.png" /> <p><small>Western blot analysis of GAPDH and HSP90 proteins in tissues (various mouse tissues) and cultured cell extracts (HeLA).</small></p> </div> <div class="small-6 medium-6 large-6 columns text-center"><img src="https://www.diagenode.com/img/applications/protein_extraction_pico.png" /> <p><small>Western blot analysis of GAPDH and ß-tubulin proteins in tissues (mouse liver) and cultured cell extracts (HeLA).</small></p> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'protein-extraction', 'meta_keywords' => 'Protein extraction,Bioruptor,Sonication,Protein Analysis', 'meta_description' => 'Diagenode provides efficient disruption and homogenization of tissues and cultured cells obtained in just one step using Bioruptor® deliver high quality protein.', 'meta_title' => 'Protein extraction using Bioruptor® Sonication device | Diagenode', 'modified' => '2017-10-16 14:39:42', 'created' => '2014-07-02 04:41:03', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '16', 'position' => '6', 'parent_id' => '5', 'name' => 'Protein Extraction', 'description' => '<div class="row"> <div class="large-12 columns">Diagenode offers optimized protein extraction kits for use with the Bioruptor<sup><span>®</span> </sup>sonication devices. The Protein Extraction Kit and Protein Extraction Beads ensure efficient disruption and homogenization of tissues in combination with the Bioruptor<sup><span>®</span></sup>.</div> </div>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'hide' => true, 'all_format' => false, 'is_antibody' => false, 'slug' => 'protein-extraction', 'cookies_tag_id' => null, 'meta_keywords' => 'Cell lysis & tissue homogenization,Bioruptor,sonication,FFPE DNA Extraction Kit, Protein Extraction Kit, and RNA Extraction Kit. ', 'meta_description' => 'Diagenode offers Cell lysis & Tissue Homogenizationa for use with the Bioruptor® Sonication Devices.', 'meta_title' => 'Cell lysis and Tissue Homogenization using Bioruptor® Sonication Devices | Diagenode', 'modified' => '2019-07-03 11:04:38', 'created' => '2015-02-07 19:59:35', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '94', 'name' => 'Protein Extraction kit manual', 'description' => '<div class="page" title="Page 4"> <div class="section"> <div class="layoutArea"> <div class="column"> <p><span>Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Efficient disruption and homogenization of animal tissues are required to ensure high yields of proteins. Diagenode’s Bioruptor</span><sup><span>® </span></sup><span>uses state-of- the-art ultrasound technology to efficiently disrupt and homogenize tissues in just one step. </span></p> </div> </div> </div> </div>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/kits/Protein_Extraction_kit_manual.pdf', 'slug' => 'protein-extraction-kit-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-07-24 17:22:14', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1776', 'name' => 'product/kits/kit-icon.png', 'alt' => 'Kit icon', 'modified' => '2019-04-09 12:06:37', 'created' => '2018-03-15 15:51:02', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( 'id' => '23', 'name' => 'Protein extraction from Tissues and Cultured Cells using Bioruptor® Standard & Plus', 'description' => '<p><span>Protein extraction from tissues and cultured cells is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Efficient disruption and homogenization of animal tissues and cultured cells are required to ensure high yields of proteins. Diagenode’s Bioruptor</span><span>® </span><span>uses state-of-the-art ultrasound technology to efficiently disrupt and homogenize tissues and cultured cells in just one step. </span></p>', 'image_id' => '213', 'type' => 'Protocol', 'url' => 'files/protocols/Protein_extraction_from_Tissues_and_cultured_cells_protocol_standard_Plus.pdf', 'slug' => 'protein-extraction-from-tissues-and-cultured-cells-protocol-standard-plus', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:40:14', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ) ), 'Publication' => array( (int) 0 => array( 'id' => '2862', 'name' => 'The Roles of the Interaction of BCL2-Antagonist/Killer 1, Apoptotic Peptidase Activating Factor 1 and Selenium in the Pathogenesis of Kashin-Beck Disease', 'authors' => 'Wang S, Duan C, Zhang F, Wang X, Guo X', 'description' => '<p>BCL2-antagonist/killer 1 (BAK1) and apoptotic peptidase activating factor 1 (APAF1) are significant genes in apoptosis signalling pathway of Kashin-Beck disease (KBD). We aimed to verify the protein expression levels of BAK1 and APAF1 in the cartilage and chondrocytes of patients with KBD. Additionally, we explored the relationship between the levels of these proteins and selenium concentration. Chondrocytes was cultured and treated with sodium selenite in vitro. Immunohistochemistry and Western blotting were used to verify the expression levels of BAK1 and APAF1. Compared with the control samples, APAF1 was upregulated and BAK1 was downregulated in the cartilage and chondrocytes of KBD patients. APAF1 expression was higher in the middle and deep zone in the KBD cartilage. APAF1 levels decreased gradually with the increasing selenium concentration (0.05, 0.10 and 0.25 mg/L). BAK1 expression in the 0.25 mg/L selenium group was lower than that of the control group. Different selenium concentrations had varying effects on BAK1 and APAF1 levels. APAF1 may play an important role in the pathogenesis of KBD. APAF1-related apoptosis was more pronounced in the middle and deep zones of the KBD cartilage. APAF may represent a potentially novel molecular target, which may be a biomarker of the role of selenium on the prevention and treatment of KBD. The role of BAK1 in the pathogenesis of KBD requires further study.</p>', 'date' => '2016-03-01', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26179084', 'doi' => '10.1007/s12011-015-0424-2', 'modified' => '2016-03-17 10:27:42', 'created' => '2016-03-17 10:27:42', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/bioruptor-plus-sonication-device"><img src="/img/product/shearing_technologies/bioruptor_plus.jpg" alt="Bioruptor Plus Sonication Device" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01020001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: QUOTE MODAL --><div id="quoteModal-1784" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Bioruptor<sup>®</sup> Plus sonication device</strong>. 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class="small-9 large-10 columns"> <input name="data[Quote][first_name]" placeholder="john" maxlength="255" type="text" id="QuoteFirstName" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Last name <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][last_name]" placeholder="doe" maxlength="255" type="text" id="QuoteLastName" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Company <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][company]" placeholder="Organisation / Institute" maxlength="255" type="text" id="QuoteCompany" required="required"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Phone number</span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][phone_number]" placeholder="+1 862 209-4680" maxlength="255" type="text" id="QuotePhoneNumber"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">City</span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][city]" placeholder="Denville" maxlength="255" type="text" id="QuoteCity"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Country <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <select name="data[Quote][country]" required="required" class="triggers" id="country_selector_quote-1784"> <option value="">-- select a country --</option> <option value="AF">Afghanistan</option> <option value="AX">Åland Islands</option> <option value="AL">Albania</option> <option value="DZ">Algeria</option> <option value="AS">American Samoa</option> <option value="AD">Andorra</option> <option value="AO">Angola</option> <option value="AI">Anguilla</option> <option value="AQ">Antarctica</option> <option value="AG">Antigua and Barbuda</option> <option value="AR">Argentina</option> <option value="AM">Armenia</option> <option value="AW">Aruba</option> <option value="AU">Australia</option> <option value="AT">Austria</option> <option value="AZ">Azerbaijan</option> <option value="BS">Bahamas</option> <option value="BH">Bahrain</option> <option value="BD">Bangladesh</option> <option value="BB">Barbados</option> <option value="BY">Belarus</option> <option value="BE">Belgium</option> <option value="BZ">Belize</option> <option value="BJ">Benin</option> <option value="BM">Bermuda</option> <option value="BT">Bhutan</option> <option value="BO">Bolivia</option> <option value="BQ">Bonaire, Sint Eustatius and Saba</option> <option value="BA">Bosnia and Herzegovina</option> <option value="BW">Botswana</option> <option value="BV">Bouvet Island</option> <option value="BR">Brazil</option> <option value="IO">British Indian Ocean Territory</option> <option value="BN">Brunei Darussalam</option> <option value="BG">Bulgaria</option> <option value="BF">Burkina Faso</option> <option value="BI">Burundi</option> <option value="KH">Cambodia</option> <option value="CM">Cameroon</option> <option value="CA">Canada</option> <option value="CV">Cape Verde</option> <option value="KY">Cayman Islands</option> <option value="CF">Central African Republic</option> <option value="TD">Chad</option> <option value="CL">Chile</option> <option value="CN">China</option> <option value="CX">Christmas Island</option> <option value="CC">Cocos (Keeling) Islands</option> <option value="CO">Colombia</option> <option value="KM">Comoros</option> <option value="CG">Congo</option> <option value="CD">Congo, The Democratic Republic of the</option> <option value="CK">Cook Islands</option> <option value="CR">Costa Rica</option> <option value="CI">Côte d'Ivoire</option> <option value="HR">Croatia</option> <option value="CU">Cuba</option> <option value="CW">Curaçao</option> <option value="CY">Cyprus</option> <option value="CZ">Czech Republic</option> <option value="DK">Denmark</option> <option value="DJ">Djibouti</option> <option value="DM">Dominica</option> <option value="DO">Dominican Republic</option> <option value="EC">Ecuador</option> <option value="EG">Egypt</option> <option value="SV">El Salvador</option> <option value="GQ">Equatorial Guinea</option> <option value="ER">Eritrea</option> <option value="EE">Estonia</option> <option value="ET">Ethiopia</option> <option value="FK">Falkland Islands (Malvinas)</option> <option value="FO">Faroe Islands</option> <option value="FJ">Fiji</option> <option value="FI">Finland</option> <option value="FR">France</option> <option value="GF">French Guiana</option> <option value="PF">French Polynesia</option> <option value="TF">French Southern Territories</option> <option value="GA">Gabon</option> <option value="GM">Gambia</option> <option value="GE">Georgia</option> <option value="DE">Germany</option> <option value="GH">Ghana</option> <option value="GI">Gibraltar</option> <option value="GR">Greece</option> <option value="GL">Greenland</option> <option value="GD">Grenada</option> <option value="GP">Guadeloupe</option> <option value="GU">Guam</option> <option value="GT">Guatemala</option> <option value="GG">Guernsey</option> <option value="GN">Guinea</option> <option value="GW">Guinea-Bissau</option> <option value="GY">Guyana</option> <option value="HT">Haiti</option> <option value="HM">Heard Island and McDonald Islands</option> <option value="VA">Holy See (Vatican City State)</option> <option value="HN">Honduras</option> <option value="HK">Hong Kong</option> <option value="HU">Hungary</option> <option value="IS">Iceland</option> <option value="IN">India</option> <option value="ID">Indonesia</option> <option value="IR">Iran, Islamic Republic of</option> <option value="IQ">Iraq</option> <option value="IE">Ireland</option> <option value="IM">Isle of Man</option> <option value="IL">Israel</option> <option value="IT">Italy</option> <option value="JM">Jamaica</option> <option value="JP">Japan</option> <option value="JE">Jersey</option> <option value="JO">Jordan</option> <option value="KZ">Kazakhstan</option> <option value="KE">Kenya</option> <option value="KI">Kiribati</option> <option value="KP">Korea, Democratic People's Republic of</option> <option value="KR">Korea, Republic of</option> <option value="KW">Kuwait</option> <option value="KG">Kyrgyzstan</option> <option value="LA">Lao People's Democratic Republic</option> <option value="LV">Latvia</option> <option value="LB">Lebanon</option> <option value="LS">Lesotho</option> <option value="LR">Liberia</option> <option value="LY">Libya</option> <option value="LI">Liechtenstein</option> <option value="LT">Lithuania</option> <option value="LU">Luxembourg</option> <option value="MO">Macao</option> <option value="MK">Macedonia, Republic of</option> <option value="MG">Madagascar</option> <option value="MW">Malawi</option> <option value="MY">Malaysia</option> <option value="MV">Maldives</option> <option value="ML">Mali</option> <option value="MT">Malta</option> <option value="MH">Marshall Islands</option> <option value="MQ">Martinique</option> <option value="MR">Mauritania</option> <option value="MU">Mauritius</option> <option value="YT">Mayotte</option> <option value="MX">Mexico</option> <option value="FM">Micronesia, Federated States of</option> <option value="MD">Moldova</option> <option value="MC">Monaco</option> <option value="MN">Mongolia</option> <option value="ME">Montenegro</option> <option value="MS">Montserrat</option> <option value="MA">Morocco</option> <option value="MZ">Mozambique</option> <option value="MM">Myanmar</option> <option value="NA">Namibia</option> <option value="NR">Nauru</option> <option value="NP">Nepal</option> <option value="NL">Netherlands</option> <option value="NC">New Caledonia</option> <option value="NZ">New Zealand</option> <option value="NI">Nicaragua</option> <option value="NE">Niger</option> <option value="NG">Nigeria</option> <option value="NU">Niue</option> <option value="NF">Norfolk Island</option> <option value="MP">Northern Mariana Islands</option> <option value="NO">Norway</option> <option value="OM">Oman</option> <option value="PK">Pakistan</option> <option value="PW">Palau</option> <option value="PS">Palestine, State of</option> <option value="PA">Panama</option> <option value="PG">Papua New Guinea</option> <option value="PY">Paraguay</option> <option value="PE">Peru</option> <option value="PH">Philippines</option> <option value="PN">Pitcairn</option> <option value="PL">Poland</option> <option value="PT">Portugal</option> <option value="PR">Puerto Rico</option> <option value="QA">Qatar</option> <option value="RE">Réunion</option> <option value="RO">Romania</option> <option value="RU">Russian Federation</option> <option value="RW">Rwanda</option> <option value="BL">Saint Barthélemy</option> <option value="SH">Saint Helena, Ascension and Tristan da Cunha</option> <option value="KN">Saint Kitts and Nevis</option> <option value="LC">Saint Lucia</option> <option value="MF">Saint Martin (French part)</option> <option value="PM">Saint Pierre and Miquelon</option> <option value="VC">Saint Vincent and the Grenadines</option> <option value="WS">Samoa</option> <option value="SM">San Marino</option> <option value="ST">Sao Tome and Principe</option> <option value="SA">Saudi Arabia</option> <option value="SN">Senegal</option> <option value="RS">Serbia</option> <option value="SC">Seychelles</option> <option value="SL">Sierra Leone</option> <option value="SG">Singapore</option> <option value="SX">Sint Maarten (Dutch part)</option> <option value="SK">Slovakia</option> <option value="SI">Slovenia</option> <option value="SB">Solomon Islands</option> <option value="SO">Somalia</option> <option value="ZA">South Africa</option> <option value="GS">South Georgia and the South Sandwich Islands</option> <option value="ES">Spain</option> <option value="LK">Sri Lanka</option> <option value="SD">Sudan</option> <option value="SR">Suriname</option> <option value="SS">South Sudan</option> <option value="SJ">Svalbard and Jan Mayen</option> <option value="SZ">Swaziland</option> <option value="SE">Sweden</option> <option value="CH">Switzerland</option> <option value="SY">Syrian Arab Republic</option> <option value="TW">Taiwan</option> <option value="TJ">Tajikistan</option> <option value="TZ">Tanzania</option> <option value="TH">Thailand</option> <option value="TL">Timor-Leste</option> <option value="TG">Togo</option> <option value="TK">Tokelau</option> <option value="TO">Tonga</option> <option value="TT">Trinidad and Tobago</option> <option value="TN">Tunisia</option> <option value="TR">Turkey</option> <option value="TM">Turkmenistan</option> <option value="TC">Turks and Caicos Islands</option> <option value="TV">Tuvalu</option> <option value="UG">Uganda</option> <option value="UA">Ukraine</option> <option value="AE">United Arab Emirates</option> <option value="GB">United Kingdom</option> <option value="US" selected="selected">United States</option> <option value="UM">United States Minor Outlying Islands</option> <option value="UY">Uruguay</option> <option value="UZ">Uzbekistan</option> <option value="VU">Vanuatu</option> <option value="VE">Venezuela</option> <option value="VN">Viet Nam</option> <option value="VG">Virgin Islands, British</option> <option value="VI">Virgin Islands, U.S.</option> <option value="WF">Wallis and Futuna</option> <option value="EH">Western Sahara</option> <option value="YE">Yemen</option> <option value="ZM">Zambia</option> <option value="ZW">Zimbabwe</option> </select><script> $('#country_selector_quote-1784').selectize(); </script><br /> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">State</span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][state]" id="state-1784" maxlength="3" type="text"/><br /> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Email <sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][email]" placeholder="email@address.com" maxlength="255" type="email" id="QuoteEmail" required="required"/> </div> </div> <div class="row collapse" id="email_v"> <div class="small-3 large-2 columns"> <span class="prefix">Email verification<sup style="font-size:16px;color:red;">*</sup></span> </div> <div class="small-9 large-10 columns"> <input name="data[Quote][email_v]" autocomplete="nope" type="text" id="QuoteEmailV"/> </div> </div> <div class="row collapse"> <div class="small-3 large-2 columns"> <span class="prefix">Comment</span> </div> <div class="small-9 large-10 columns"> <textarea name="data[Quote][comment]" placeholder="Additional comments" cols="30" rows="6" id="QuoteComment"></textarea> </div> </div> <!------------SERVICES PARTICULAR FORM START----------------> <!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES-----> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <div class="recaptcha"><div id="recaptcha673f50ed8f71e"></div></div> </div> </div> <br /> <div class="row collapse"> <div class="small-3 large-2 columns"> </div> <div class="small-9 large-10 columns"> <button id="submit_btn-1784" class="alert button expand" form="Quote-1784" type="submit">Contact me</button> </div> </div> </form><script> var pardotFormHandlerURL = 'https://go.diagenode.com/l/928883/2022-10-10/36b1c'; function postToPardot(formAction, id) { $('#pardot-form-handler').load(function(){ $('#Quote-' + id).attr('action', formAction); $('#Quote-' + id).submit(); }); $('#pardot-form-handler').attr('src', pardotFormHandlerURL + '?' + $('#Quote-' + id).serialize()); } $(document).ready(function() { $('body').append('<iframe id="pardot-form-handler" height="0" width="0" style="position:absolute; left:-100000px; top:-100000px" src="javascript:false;"></iframe>'); $('#Quote-1784').attr('action','javascript:postToPardot(\'' + $('#Quote-1784').attr('action') + '\', 1784)'); }); $("#Quote-1784 #submit_btn-1784").click(function (e) { if($(this).closest('form')[0].checkValidity()){ e.preventDefault(); //disable the submit button $("#Quote-1784 #submit_btn-1784").attr("disabled", true); $("#Quote-1784 #submit_btn-1784").html("Processing..."); //submit the form $("#Quote-1784").submit(); } }) </script> <script> if ($("#Quote-1784 #country_selector_quote-1784.selectized").val() == 'US') { var val = $("#state-1784").val(); $("#Quote-1784 #state-1784").replaceWith('<select name="data[Quote][state]" id="state-1784" required><option disabled selected value> -- select a state -- </option><option value="AL">Alabama (AL)</option><option value="AK">Alaska (AK)</option><option value="AZ">Arizona 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value="PE">Prince Edward Island (PE)</option><option value="QC">Quebec (QC)</option><option value="SK">Saskatchewan (SK)</option></select>'); $("#Quote-1784 #state-1784").parent().parent().show(); } else if (this.value == 'DE') { $("#Quote-1784 #state-1784").replaceWith('<select name="data[Quote][state]" id="state-1784" required><option disabled selected value> -- select a state -- </option><option value="BW">Baden-Württemberg (BW)</option><option value="BY">Bayern (BY)</option><option value="BE">Berlin (BE)</option><option value="BB">Brandeburg (BB)</option><option value="HB">Bremen (HB)</option><option value="HH">Hamburg (HH)</option><option value="HE">Hessen (HE)</option><option value="MV">Mecklenburg-Vorpommern (MV)</option><option value="NI">Niedersachsen (NI)</option><option value="NW">Nordrhein-Westfalen (NW)</option><option value="RP">Rheinland-Pfalz (RP)</option><option value="SL">Saarland (SL)</option><option value="SN">Sachsen (SN)</option><option value ="SA">Sachsen-Anhalt (SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>'); $("#Quote-1784 #state-1784").parent().parent().show(); } else { $("#Quote-1784 #state-1784").parent().parent().hide(); $("#Quote-1784 #state-1784").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-1784" value="">'); } }); </script> <a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: QUOTE MODAL --><a href="#" id="bioruptor-plus-sonication-device" data-reveal-id="quoteModal-1784" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Bioruptor® Plus sonication device</h6> </div> </div> </li> <li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack"><img src="/img/product/shearing_technologies/B01200016_tube_holder.jpg" alt="some alt" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01200016</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1796" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/1796" id="CartAdd/1796Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1796" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico', 'B01200016', '350', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico', 'B01200016', '350', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack" 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We aimed to verify the protein expression levels of BAK1 and APAF1 in the cartilage and chondrocytes of patients with KBD. Additionally, we explored the relationship between the levels of these proteins and selenium concentration. Chondrocytes was cultured and treated with sodium selenite in vitro. Immunohistochemistry and Western blotting were used to verify the expression levels of BAK1 and APAF1. Compared with the control samples, APAF1 was upregulated and BAK1 was downregulated in the cartilage and chondrocytes of KBD patients. APAF1 expression was higher in the middle and deep zone in the KBD cartilage. APAF1 levels decreased gradually with the increasing selenium concentration (0.05, 0.10 and 0.25 mg/L). BAK1 expression in the 0.25 mg/L selenium group was lower than that of the control group. Different selenium concentrations had varying effects on BAK1 and APAF1 levels. APAF1 may play an important role in the pathogenesis of KBD. APAF1-related apoptosis was more pronounced in the middle and deep zones of the KBD cartilage. APAF may represent a potentially novel molecular target, which may be a biomarker of the role of selenium on the prevention and treatment of KBD. 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The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p> </div> </div> <p></p> <p><span></span></p> <div class="page" title="Page 9"> <div class="section"> <div class="layoutArea"> <div class="column"></div> </div> </div> </div>', 'label1' => 'Which Bioruptor® Plus configuration is right for you?', 'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p> <p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p> <p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>', 'label2' => 'Available chromatin shearing kits', 'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => 'View accessories & consumables for Bioruptor<sup>®</sup> Plus', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; 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The Protein Extraction Kit and Protein Extraction Beads ensure efficient disruption and homogenization of tissues in combination with the Bioruptor<sup><span>®</span></sup>.</div> </div>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'hide' => true, 'all_format' => false, 'is_antibody' => false, 'slug' => 'protein-extraction', 'cookies_tag_id' => null, 'meta_keywords' => 'Cell lysis & tissue homogenization,Bioruptor,sonication,FFPE DNA Extraction Kit, Protein Extraction Kit, and RNA Extraction Kit. ', 'meta_description' => 'Diagenode offers Cell lysis & Tissue Homogenizationa for use with the Bioruptor® Sonication Devices.', 'meta_title' => 'Cell lysis and Tissue Homogenization using Bioruptor® Sonication Devices | Diagenode', 'modified' => '2019-07-03 11:04:38', 'created' => '2015-02-07 19:59:35', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '94', 'name' => 'Protein Extraction kit manual', 'description' => '<div class="page" title="Page 4"> <div class="section"> <div class="layoutArea"> <div class="column"> <p><span>Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Efficient disruption and homogenization of animal tissues are required to ensure high yields of proteins. Diagenode’s Bioruptor</span><sup><span>® </span></sup><span>uses state-of- the-art ultrasound technology to efficiently disrupt and homogenize tissues in just one step. </span></p> </div> </div> </div> </div>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/kits/Protein_Extraction_kit_manual.pdf', 'slug' => 'protein-extraction-kit-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-07-24 17:22:14', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1776', 'name' => 'product/kits/kit-icon.png', 'alt' => 'Kit icon', 'modified' => '2019-04-09 12:06:37', 'created' => '2018-03-15 15:51:02', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( 'id' => '23', 'name' => 'Protein extraction from Tissues and Cultured Cells using Bioruptor® Standard & Plus', 'description' => '<p><span>Protein extraction from tissues and cultured cells is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Efficient disruption and homogenization of animal tissues and cultured cells are required to ensure high yields of proteins. Diagenode’s Bioruptor</span><span>® </span><span>uses state-of-the-art ultrasound technology to efficiently disrupt and homogenize tissues and cultured cells in just one step. </span></p>', 'image_id' => '213', 'type' => 'Protocol', 'url' => 'files/protocols/Protein_extraction_from_Tissues_and_cultured_cells_protocol_standard_Plus.pdf', 'slug' => 'protein-extraction-from-tissues-and-cultured-cells-protocol-standard-plus', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:40:14', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ) ), 'Publication' => array( (int) 0 => array( 'id' => '2862', 'name' => 'The Roles of the Interaction of BCL2-Antagonist/Killer 1, Apoptotic Peptidase Activating Factor 1 and Selenium in the Pathogenesis of Kashin-Beck Disease', 'authors' => 'Wang S, Duan C, Zhang F, Wang X, Guo X', 'description' => '<p>BCL2-antagonist/killer 1 (BAK1) and apoptotic peptidase activating factor 1 (APAF1) are significant genes in apoptosis signalling pathway of Kashin-Beck disease (KBD). We aimed to verify the protein expression levels of BAK1 and APAF1 in the cartilage and chondrocytes of patients with KBD. Additionally, we explored the relationship between the levels of these proteins and selenium concentration. Chondrocytes was cultured and treated with sodium selenite in vitro. Immunohistochemistry and Western blotting were used to verify the expression levels of BAK1 and APAF1. Compared with the control samples, APAF1 was upregulated and BAK1 was downregulated in the cartilage and chondrocytes of KBD patients. APAF1 expression was higher in the middle and deep zone in the KBD cartilage. APAF1 levels decreased gradually with the increasing selenium concentration (0.05, 0.10 and 0.25 mg/L). BAK1 expression in the 0.25 mg/L selenium group was lower than that of the control group. Different selenium concentrations had varying effects on BAK1 and APAF1 levels. APAF1 may play an important role in the pathogenesis of KBD. APAF1-related apoptosis was more pronounced in the middle and deep zones of the KBD cartilage. APAF may represent a potentially novel molecular target, which may be a biomarker of the role of selenium on the prevention and treatment of KBD. The role of BAK1 in the pathogenesis of KBD requires further study.</p>', 'date' => '2016-03-01', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26179084', 'doi' => '10.1007/s12011-015-0424-2', 'modified' => '2016-03-17 10:27:42', 'created' => '2016-03-17 10:27:42', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/bioruptor-plus-sonication-device"><img src="/img/product/shearing_technologies/bioruptor_plus.jpg" alt="Bioruptor Plus Sonication Device" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01020001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: QUOTE MODAL --><div id="quoteModal-1784" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Bioruptor<sup>®</sup> Plus sonication device</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p> </div> </div> <form action="/jp/quotes/quote?id=1784" id="Quote-1784" class="quote" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Quote][product_id]" value="1784" id="QuoteProductId"/><input type="hidden" name="data[Quote][formLoaded6tY4bPYk]" value="V3o0cTFiMEtpL2QxZTNoOWdnS2FEdz09" id="QuoteFormLoaded6tY4bPYk"/><input type="hidden" name="data[Quote][product_rfq_tag]" value="bioruptor-plus-sonication-device" id="QuoteProductRfqTag"/><input type="hidden" name="data[Quote][source_quote]" value="modal quote" id="QuoteSourceQuote"/> <div class="row collapse"> <h2>Contact Information</h2> <div class="small-3 large-2 columns"> <span class="prefix">First name <sup style="font-size:16px;color:red;">*</sup></span> </div> <div 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</div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01200016</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1796" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/1796" id="CartAdd/1796Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1796" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico', 'B01200016', '350', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico', 'B01200016', '350', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack" data-reveal-id="cartModal-1796" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">15 ml sonication accessories for Picoruptor</h6> </div> </div> </li> ' $related = array( 'id' => '1796', 'antibody_id' => null, 'name' => '15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico', 'description' => '<p><span>This tube holder has been designed for being used in combination with the Bioruptor® Standard, Plus and Pico for tissue disruption and cell lysis. </span></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script 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Efficient disruption and homogenization of animal tissues are required to ensure high yields of proteins. Diagenode’s Bioruptor</span><sup><span>® </span></sup><span>uses state-of- the-art ultrasound technology to efficiently disrupt and homogenize tissues in just one step. </span></p> </div> </div> </div> </div>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/kits/Protein_Extraction_kit_manual.pdf', 'slug' => 'protein-extraction-kit-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-07-24 17:22:14', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( 'id' => '740', 'product_id' => '2617', 'document_id' => '94' ) ) $publication = array( 'id' => '2862', 'name' => 'The Roles of the Interaction of BCL2-Antagonist/Killer 1, Apoptotic Peptidase Activating Factor 1 and Selenium in the Pathogenesis of Kashin-Beck Disease', 'authors' => 'Wang S, Duan C, Zhang F, Wang X, Guo X', 'description' => '<p>BCL2-antagonist/killer 1 (BAK1) and apoptotic peptidase activating factor 1 (APAF1) are significant genes in apoptosis signalling pathway of Kashin-Beck disease (KBD). We aimed to verify the protein expression levels of BAK1 and APAF1 in the cartilage and chondrocytes of patients with KBD. Additionally, we explored the relationship between the levels of these proteins and selenium concentration. Chondrocytes was cultured and treated with sodium selenite in vitro. Immunohistochemistry and Western blotting were used to verify the expression levels of BAK1 and APAF1. Compared with the control samples, APAF1 was upregulated and BAK1 was downregulated in the cartilage and chondrocytes of KBD patients. APAF1 expression was higher in the middle and deep zone in the KBD cartilage. APAF1 levels decreased gradually with the increasing selenium concentration (0.05, 0.10 and 0.25 mg/L). BAK1 expression in the 0.25 mg/L selenium group was lower than that of the control group. Different selenium concentrations had varying effects on BAK1 and APAF1 levels. APAF1 may play an important role in the pathogenesis of KBD. APAF1-related apoptosis was more pronounced in the middle and deep zones of the KBD cartilage. APAF may represent a potentially novel molecular target, which may be a biomarker of the role of selenium on the prevention and treatment of KBD. The role of BAK1 in the pathogenesis of KBD requires further study.</p>', 'date' => '2016-03-01', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26179084', 'doi' => '10.1007/s12011-015-0424-2', 'modified' => '2016-03-17 10:27:42', 'created' => '2016-03-17 10:27:42', 'ProductsPublication' => array( 'id' => '567', 'product_id' => '2617', 'publication_id' => '2862' ) ) $externalLink = ' <a href="http://www.ncbi.nlm.nih.gov/pubmed/26179084" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table>', 'label3' => 'View accessories & consumables for Bioruptor<sup>®</sup> Plus', 'info3' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td> <td style="width: 160px; 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height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; 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The Protein Extraction Kit and Protein Extraction Beads ensure efficient disruption and homogenization of tissues in combination with the Bioruptor<sup><span>®</span></sup>.</div> </div>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'hide' => true, 'all_format' => false, 'is_antibody' => false, 'slug' => 'protein-extraction', 'cookies_tag_id' => null, 'meta_keywords' => 'Cell lysis & tissue homogenization,Bioruptor,sonication,FFPE DNA Extraction Kit, Protein Extraction Kit, and RNA Extraction Kit. ', 'meta_description' => 'Diagenode offers Cell lysis & Tissue Homogenizationa for use with the Bioruptor® Sonication Devices.', 'meta_title' => 'Cell lysis and Tissue Homogenization using Bioruptor® Sonication Devices | Diagenode', 'modified' => '2019-07-03 11:04:38', 'created' => '2015-02-07 19:59:35', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '94', 'name' => 'Protein Extraction kit manual', 'description' => '<div class="page" title="Page 4"> <div class="section"> <div class="layoutArea"> <div class="column"> <p><span>Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Efficient disruption and homogenization of animal tissues are required to ensure high yields of proteins. Diagenode’s Bioruptor</span><sup><span>® </span></sup><span>uses state-of- the-art ultrasound technology to efficiently disrupt and homogenize tissues in just one step. </span></p> </div> </div> </div> </div>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/kits/Protein_Extraction_kit_manual.pdf', 'slug' => 'protein-extraction-kit-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-07-24 17:22:14', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '1776', 'name' => 'product/kits/kit-icon.png', 'alt' => 'Kit icon', 'modified' => '2019-04-09 12:06:37', 'created' => '2018-03-15 15:51:02', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( 'id' => '23', 'name' => 'Protein extraction from Tissues and Cultured Cells using Bioruptor® Standard & Plus', 'description' => '<p><span>Protein extraction from tissues and cultured cells is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Efficient disruption and homogenization of animal tissues and cultured cells are required to ensure high yields of proteins. Diagenode’s Bioruptor</span><span>® </span><span>uses state-of-the-art ultrasound technology to efficiently disrupt and homogenize tissues and cultured cells in just one step. </span></p>', 'image_id' => '213', 'type' => 'Protocol', 'url' => 'files/protocols/Protein_extraction_from_Tissues_and_cultured_cells_protocol_standard_Plus.pdf', 'slug' => 'protein-extraction-from-tissues-and-cultured-cells-protocol-standard-plus', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:40:14', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( [maximum depth reached] ) ) ), 'Publication' => array( (int) 0 => array( 'id' => '2862', 'name' => 'The Roles of the Interaction of BCL2-Antagonist/Killer 1, Apoptotic Peptidase Activating Factor 1 and Selenium in the Pathogenesis of Kashin-Beck Disease', 'authors' => 'Wang S, Duan C, Zhang F, Wang X, Guo X', 'description' => '<p>BCL2-antagonist/killer 1 (BAK1) and apoptotic peptidase activating factor 1 (APAF1) are significant genes in apoptosis signalling pathway of Kashin-Beck disease (KBD). We aimed to verify the protein expression levels of BAK1 and APAF1 in the cartilage and chondrocytes of patients with KBD. Additionally, we explored the relationship between the levels of these proteins and selenium concentration. Chondrocytes was cultured and treated with sodium selenite in vitro. Immunohistochemistry and Western blotting were used to verify the expression levels of BAK1 and APAF1. Compared with the control samples, APAF1 was upregulated and BAK1 was downregulated in the cartilage and chondrocytes of KBD patients. APAF1 expression was higher in the middle and deep zone in the KBD cartilage. APAF1 levels decreased gradually with the increasing selenium concentration (0.05, 0.10 and 0.25 mg/L). BAK1 expression in the 0.25 mg/L selenium group was lower than that of the control group. Different selenium concentrations had varying effects on BAK1 and APAF1 levels. APAF1 may play an important role in the pathogenesis of KBD. APAF1-related apoptosis was more pronounced in the middle and deep zones of the KBD cartilage. APAF may represent a potentially novel molecular target, which may be a biomarker of the role of selenium on the prevention and treatment of KBD. The role of BAK1 in the pathogenesis of KBD requires further study.</p>', 'date' => '2016-03-01', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26179084', 'doi' => '10.1007/s12011-015-0424-2', 'modified' => '2016-03-17 10:27:42', 'created' => '2016-03-17 10:27:42', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/bioruptor-plus-sonication-device"><img src="/img/product/shearing_technologies/bioruptor_plus.jpg" alt="Bioruptor Plus Sonication Device" class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01020001</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: QUOTE MODAL --><div id="quoteModal-1784" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Bioruptor<sup>®</sup> Plus sonication device</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p> </div> </div> <form action="/jp/quotes/quote?id=1784" id="Quote-1784" class="quote" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Quote][product_id]" value="1784" id="QuoteProductId"/><input type="hidden" name="data[Quote][formLoaded6tY4bPYk]" value="V3o0cTFiMEtpL2QxZTNoOWdnS2FEdz09" id="QuoteFormLoaded6tY4bPYk"/><input type="hidden" name="data[Quote][product_rfq_tag]" value="bioruptor-plus-sonication-device" id="QuoteProductRfqTag"/><input type="hidden" name="data[Quote][source_quote]" value="modal quote" id="QuoteSourceQuote"/> <div class="row collapse"> <h2>Contact Information</h2> <div class="small-3 large-2 columns"> <span class="prefix">First name <sup style="font-size:16px;color:red;">*</sup></span> </div> <div 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</div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01200016</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1796" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"> <form action="/jp/carts/add/1796" id="CartAdd/1796Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1796" id="CartProductId"/> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico個カートに追加。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico', 'B01200016', '350', $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button> </div> <div class="small-6 medium-6 large-6 columns"> <button class="alert small button expand" onclick="$(this).addToCart('15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico', 'B01200016', '350', $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button> </div> </div> </div> </div> </form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack" data-reveal-id="cartModal-1796" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">15 ml sonication accessories for Picoruptor</h6> </div> </div> </li> ' $related = array( 'id' => '1796', 'antibody_id' => null, 'name' => '15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico', 'description' => '<p><span>This tube holder has been designed for being used in combination with the Bioruptor® Standard, Plus and Pico for tissue disruption and cell lysis. </span></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script 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} $old_catalog_number = '<br/><small><span style="color:#CCC">(AL-002-0050)</span></small>' $country_code = 'US' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $protocol = array( 'id' => '23', 'name' => 'Protein extraction from Tissues and Cultured Cells using Bioruptor® Standard & Plus', 'description' => '<p><span>Protein extraction from tissues and cultured cells is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Efficient disruption and homogenization of animal tissues and cultured cells are required to ensure high yields of proteins. Diagenode’s Bioruptor</span><span>® </span><span>uses state-of-the-art ultrasound technology to efficiently disrupt and homogenize tissues and cultured cells in just one step. </span></p>', 'image_id' => '213', 'type' => 'Protocol', 'url' => 'files/protocols/Protein_extraction_from_Tissues_and_cultured_cells_protocol_standard_Plus.pdf', 'slug' => 'protein-extraction-from-tissues-and-cultured-cells-protocol-standard-plus', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-04-29 17:40:14', 'created' => '2015-07-20 10:35:07', 'ProductsProtocol' => array( 'id' => '80', 'product_id' => '2617', 'protocol_id' => '23' ) ) $document = array( 'id' => '94', 'name' => 'Protein Extraction kit manual', 'description' => '<div class="page" title="Page 4"> <div class="section"> <div class="layoutArea"> <div class="column"> <p><span>Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Efficient disruption and homogenization of animal tissues are required to ensure high yields of proteins. Diagenode’s Bioruptor</span><sup><span>® </span></sup><span>uses state-of- the-art ultrasound technology to efficiently disrupt and homogenize tissues in just one step. </span></p> </div> </div> </div> </div>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/kits/Protein_Extraction_kit_manual.pdf', 'slug' => 'protein-extraction-kit-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-07-24 17:22:14', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( 'id' => '740', 'product_id' => '2617', 'document_id' => '94' ) ) $publication = array( 'id' => '2862', 'name' => 'The Roles of the Interaction of BCL2-Antagonist/Killer 1, Apoptotic Peptidase Activating Factor 1 and Selenium in the Pathogenesis of Kashin-Beck Disease', 'authors' => 'Wang S, Duan C, Zhang F, Wang X, Guo X', 'description' => '<p>BCL2-antagonist/killer 1 (BAK1) and apoptotic peptidase activating factor 1 (APAF1) are significant genes in apoptosis signalling pathway of Kashin-Beck disease (KBD). We aimed to verify the protein expression levels of BAK1 and APAF1 in the cartilage and chondrocytes of patients with KBD. Additionally, we explored the relationship between the levels of these proteins and selenium concentration. Chondrocytes was cultured and treated with sodium selenite in vitro. Immunohistochemistry and Western blotting were used to verify the expression levels of BAK1 and APAF1. Compared with the control samples, APAF1 was upregulated and BAK1 was downregulated in the cartilage and chondrocytes of KBD patients. APAF1 expression was higher in the middle and deep zone in the KBD cartilage. APAF1 levels decreased gradually with the increasing selenium concentration (0.05, 0.10 and 0.25 mg/L). BAK1 expression in the 0.25 mg/L selenium group was lower than that of the control group. Different selenium concentrations had varying effects on BAK1 and APAF1 levels. APAF1 may play an important role in the pathogenesis of KBD. APAF1-related apoptosis was more pronounced in the middle and deep zones of the KBD cartilage. APAF may represent a potentially novel molecular target, which may be a biomarker of the role of selenium on the prevention and treatment of KBD. The role of BAK1 in the pathogenesis of KBD requires further study.</p>', 'date' => '2016-03-01', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26179084', 'doi' => '10.1007/s12011-015-0424-2', 'modified' => '2016-03-17 10:27:42', 'created' => '2016-03-17 10:27:42', 'ProductsPublication' => array( 'id' => '567', 'product_id' => '2617', 'publication_id' => '2862' ) ) $externalLink = ' <a href="http://www.ncbi.nlm.nih.gov/pubmed/26179084" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
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