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PspCas13b monoclonal antibody

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Catalog Number

Format

Price

C15200250
(C15200244)

50 µg

$465.00
Bulk order

Other format

C15200250-10

10 μg

Monoclonal antibody raised in mouse against the Cas13b protein from Prevotella sp. (CRISPR associated protein 13a) using a recombinant protein.

Lot	002
Concentration	2 µg/µl
Species reactivity	Prevotella sp.
Type	Monoclonal
Purity	Protein G purified monoclonal antibody.
Host	Mouse
Storage Conditions	Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage Buffer	TBS containing 0.02 % Na-azide.
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
Western blotting	1:1,000 – 1:4,000	Fig 1, 2

Validation data
CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.
Validation data

Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b
Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length LwCas13a (lane 2) or PspCas13b (lane 3), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:2,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.

Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b
Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.

資料
PspCas13b monoclonal antibody DATASHEET
Monoclonal antibody raised in mouse against the Cas13b protein from Prevotella sp. (CRISPR associ... Download

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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length LwCas13a (lane 2) or PspCas13b (lane 3), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:2,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length LwCas13a (lane 2) or PspCas13b (lane 3), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:2,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length LwCas13a (lane 2) or PspCas13b (lane 3), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:2,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length LwCas13a (lane 2) or PspCas13b (lane 3), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:2,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length LwCas13a (lane 2) or PspCas13b (lane 3), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:2,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br />Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length Cas13b (lane 2), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:1,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right. The bottom panel shows the result of a WB performed with an actin antibody, used as a loading control.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br />Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
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<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length Cas13b (lane 2), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:1,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right. The bottom panel shows the result of a WB performed with an actin antibody, used as a loading control.</small></p>
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<div class="small-8 columns">
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length LwCas13a (lane 2) or PspCas13b (lane 3), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:2,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br />Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length Cas13b (lane 2), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:1,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right. The bottom panel shows the result of a WB performed with an actin antibody, used as a loading control.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br />Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length Cas13b (lane 2), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:1,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right. The bottom panel shows the result of a WB performed with an actin antibody, used as a loading control.</small></p>
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<div class="small-8 columns">
<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br />Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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			'info1' => '<p>CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9 nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called class 2 type VI CRISPR system has been identified which is characterized by the presence of the single effector protein. Cas13b is one of the three proteins belonging to the class 2 type VI system identified so far. These proteins lack homology to other DNA nuclease domains but contain two Higher Eukaryotes and Prokaryotes Nucleotide-binding (HEPN) domains and are thought to function solely as an RNA-guided RNA-targeting CRISPR effector.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig1-WB.png" alt="WB result" /></p>
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<div class="small-8 columns">
<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length LwCas13a (lane 2) or PspCas13b (lane 3), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:2,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200250-fig2-WB.png" alt="WB result" /></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br /> Western blot was performed on protein extracts from HEK293 cells (lane 1) and from HEK293 cells transiently expressing full-length Cas13b (lane 2), using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was diluted 1:1,000 in PBS-T containing 0.5% NFDM. The marker is shown on the left, the position of the Cas13b protein is indicated on the right. The bottom panel shows the result of a WB performed with an actin antibody, used as a loading control.</small></p>
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<p><small><strong>Figure 2. Western blot analysis using the Diagenode monoclonal antibody directed against Cas13b</strong><br />Western blot was performed on protein extracts from HEK293 cells transiently expressing full-length Cas13b, using the Diagenode antibody against Cas13b (cat. No. C15200250). The antibody was used at different dilutions. The marker is shown on the left, the position of the Cas13b protein is indicated on the right.</small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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