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The Rat IgG has been extensively validated in hydroxymethylated DNA IP (hMeDIP) assays. It contains a spectrum of the IgG subclasses present in serum of healthy animals. This Rat IgG preparation is intended for being used in hMeDIP experiments (Cat. No. AF-104-0016) as a negative control in parallel with the specific monoclonal 5-hydroxymethylcytosine antibody (Cat. No. MAb-633HMC-100). Both antibodies should be used at the same concentration.
| Lot | D001 |
|---|---|
| Concentration | 1.0 µg/µl |
| Species reactivity | none |
| Type | Polyclonal |
| Purity | Protein A purified |
| Host | Rat |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Figure 1. hMeDIP with the Diagenode rat IgG negative control antibody
hMeDIP assays were performed using the Diagenode rat monoclonal antibody against 5-hmC (cat. No. C15220001) and the “hMeDIP” kit (rat, cat. No. C02010030) on 1 μg of sheared DNA from mouse ES cells. The DNA was spiked with 0.025 ng of each of the DNA standards from the 5-hmC, 5-mC & cytosine DNA standard pack (cat. No. C02040011), prior to the IP Rat IgG (cat. No. C15420001) was used as a negative IP control. 2.5 μg of antibody per ChIP experiment was used for both antibodies. Quantitative PCR was performed with the primers specific for the unmethylated, methylated and hydroxymethylated controls present in the kit. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
| メチル化DNA免疫沈降 hMeDIPの場合、メチル化DNA IP（MeDIP）は5-メチルシトシン（5-mC）または5-ヒドロキシメチルシトシン（5-hmC）に対する抗体を用いたメチル化DNAのアフィニティー精製に基づきます。 使い方 メチルDNA IPは以下のようにして実施されます。(要約)：培養細胞または組織からのゲノムDNAを調製、せん断、その後変性させます。 次いで、免疫選択および免疫沈降を、5メチルシトシンに対する抗体および抗体結合ビーズを用いて行うことが可能です。 単離および精製が行われた後、... Read more |
| Datasheet ratIgG AF-105-0025 DATASHEET Datasheet description | Download |
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Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo |
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This Rat IgG preparation is intended for being used in </span><a href="../p/hmedip-kit-x16-monoclonal-rat-antibody-16-rxns">hMeDIP experiments (Cat. No. AF-104-0016)</a><span> as a negative control in parallel with the specific monoclonal </span><a href="../p/5-hmc-monoclonal-antibody-rat-classic-100-ug-64-ul">5-hydroxymethylcytosine antibody (Cat. No. MAb-633HMC-100)</a><span>. Both antibodies should be used at the same concentration.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15420001-hmedip.jpg" alt="hMeDIP" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. hMeDIP with the Diagenode rat IgG negative control antibody</strong><br />hMeDIP assays were performed using the Diagenode rat monoclonal antibody against 5-hmC (cat. No. C15220001) and the “hMeDIP” kit (rat, cat. No. C02010030) on 1 μg of sheared DNA from mouse ES cells. 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It contains a spectrum of the IgG subclasses present in serum of healthy animals. This Rat IgG preparation is intended for being used in </span><a href="../p/hmedip-kit-x16-monoclonal-rat-antibody-16-rxns">hMeDIP experiments (Cat. No. AF-104-0016)</a><span> as a negative control in parallel with the specific monoclonal </span><a href="../p/5-hmc-monoclonal-antibody-rat-classic-100-ug-64-ul">5-hydroxymethylcytosine antibody (Cat. No. MAb-633HMC-100)</a><span>. Both antibodies should be used at the same concentration.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15420001-hmedip.jpg" alt="hMeDIP" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. hMeDIP with the Diagenode rat IgG negative control antibody</strong><br />hMeDIP assays were performed using the Diagenode rat monoclonal antibody against 5-hmC (cat. 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Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '25 µg/ 25 µl', 'catalog_number' => 'C15420001', 'old_catalog_number' => 'AF-105-0025', 'sf_code' => 'C15420001-364', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '65', 'price_USD' => '90', 'price_GBP' => '65', 'price_JPY' => '10180', 'price_CNY' => '', 'price_AUD' => '225', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'rat-igg-25-ug-25-ul', 'meta_title' => 'Rat IgG', 'meta_keywords' => '', 'meta_description' => 'Rat IgG', 'modified' => '2016-06-21 14:10:35', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '329', 'name' => 'Rat IgG', 'description' => 'The Rat IgG has been extensively validated in hydroxymethylated DNA IP (hMeDIP) assays. 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Then, immunoselection and immunoprecipitation can take place using the antibody directed against 5 methylcytosine and antibody binding beads. After isolation and purification is performed, the IP’d methylated DNA is ready for any subsequent analysis as qPCR, amplification, hybridization on microarrays or next generation sequencing.</p> <h2>Applications</h2> <div align="center"><a href="https://www.diagenode.com/en/p/magmedip-kit-x48-48-rxns" class="center alert radius button"> qPCR analysis</a></div> <div align="center"><a href="https://www.diagenode.com/en/p/magmedip-seq-package-V2-x10" class="center alert radius button"> NGS analysis </a></div> <h2>Advantages</h2> <ul style="font-size: 19px;" class="nobullet"> <li><i class="fa fa-arrow-circle-right"></i> <strong>Unaffected</strong> DNA</li> <li><i class="fa fa-arrow-circle-right"></i> <strong>High enrichment</strong> yield</li> <li><i class="fa fa-arrow-circle-right"></i> <strong>Robust</strong> & <strong>reproducible</strong> techniques</li> <li><i class="fa fa-arrow-circle-right"></i> <strong>NGS</strong> compatible</li> </ul> <h2></h2> </div> </div> <div id="gtx-trans" style="position: absolute; left: 17px; top: 652.938px;"> <div class="gtx-trans-icon"></div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'methylated-dna-immunoprecipitation', 'meta_keywords' => 'Methylated DNA immunoprecipitation,Epigenetic,DNA Methylation,qPCR,5 methylcytosine (5-mC)', 'meta_description' => 'Methylated DNA immunoprecipitation method is based on the affinity purification of methylated DNA using an antibody directed against 5 methylcytosine (5-mC). ', 'meta_title' => 'Methylated DNA immunoprecipitation(MeDIP) - Dna methylation | Diagenode', 'modified' => '2021-08-19 12:08:03', 'created' => '2014-09-14 05:33:34', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '22', 'position' => '40', 'parent_id' => '4', 'name' => 'Isotype controls', 'description' => '<p><span style="font-weight: 400;">Diagenode offers the negative Ctrl IgG from rabbit, rat and mouse. These extensively validated antibodies can be used as negative controls in ChIP, IF, hMeDIP or other experiments performed with specific antibodies made in rabbit, rat or mouse, respectively.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'isotype-controls', 'cookies_tag_id' => null, 'meta_keywords' => 'Isotype controls,DNA immunoprecipitation,Methylated DNA immunoprecipitation', 'meta_description' => 'Diagenode provides Isotype controls for Methylated DNA immunoprecipitation', 'meta_title' => 'Isotype controls for Methylated DNA immunoprecipitation | Diagenode', 'modified' => '2019-07-04 16:19:36', 'created' => '2014-09-30 14:23:34', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. 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Selective transcriptional activation and silencing of genes mediate the response of plants to environmental signals and developmental cues. Therefore, insights into the mechanisms that control plant gene expression are essential to gain a deep understanding of how biological processes are regulated in plants. The chromatin immunoprecipitation (ChIP) technique described here is a procedure to identify the DNA-binding sites of proteins in genes or genomic regions of the model species Arabidopsis thaliana. The interactions with DNA of proteins of interest such as transcription factors, chromatin proteins or posttranslationally modified versions of histones can be efficiently analyzed with the ChIP protocol. This method is based on the fixation of protein-DNA interactions in vivo, random fragmentation of chromatin, immunoprecipitation of protein-DNA complexes with specific antibodies, and quantification of the DNA associated with the protein of interest by PCR techniques. The use of this methodology in Arabidopsis has contributed significantly to unveil transcriptional regulatory mechanisms that control a variety of plant biological processes. This approach allowed the identification of the binding sites of the Arabidopsis chromatin protein EBS to regulatory regions of the master gene of flowering FT. The impact of this protein in the accumulation of particular histone marks in the genomic region of FT was also revealed through ChIP analysis.</p>', 'date' => '2016-01-14', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26863263', 'doi' => '10.3791/53422', 'modified' => '2017-01-04 14:16:52', 'created' => '2016-03-09 17:05:45', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '470', 'name' => 'Rat IgG SDS GB en', 'language' => 'en', 'url' => 'files/SDS/Rat_IgG/SDS-C15420001-Rat_IgG-GB-en-GHS_2_0.pdf', 'countries' => 'GB', 'modified' => '2020-07-01 11:04:39', 'created' => '2020-07-01 11:04:39', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '472', 'name' => 'Rat IgG SDS US en', 'language' => 'en', 'url' => 'files/SDS/Rat_IgG/SDS-C15420001-Rat_IgG-US-en-GHS_2_0.pdf', 'countries' => 'US', 'modified' => '2020-07-01 11:05:29', 'created' => 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=> '10', 'parent_id' => '1', 'name' => 'Methylated DNA immunoprecipitation', 'description' => '<div class="row extra-spaced"> <div class="small-12 medium-3 large-3 columns"><center><a href="https://www.ncbi.nlm.nih.gov/pubmed/30429608" target="_blank"><img src="https://www.diagenode.com/img/banners/banner-nature-publication-580.png" /></a></center></div> <div class="small-12 medium-9 large-9 columns"> <h3>Sensitive tumour detection and classification using plasma cell-free DNA methylomes<br /><a href="https://www.ncbi.nlm.nih.gov/pubmed/30429608" target="_blank">Read the publication</a></h3> <h3 class="c-article-title u-h1" data-test="article-title" itemprop="name headline">Preparation of cfMeDIP-seq libraries for methylome profiling of plasma cell-free DNA<br /><a href="https://www.nature.com/articles/s41596-019-0202-2" target="_blank" title="cfMeDIP-seq Nature Method">Read the method</a></h3> </div> </div> <div class="row"> <div class="large-12 columns"><span>The Methylated DNA Immunoprecipitation is based on the affinity purification of methylated and hydroxymethylated DNA using, respectively, an antibody directed against 5-methylcytosine (5-mC) in the case of MeDIP or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP.</span><br /> <h2></h2> <h2>How it works</h2> <p>In brief, Methyl DNA IP is performed as follows: Genomic DNA from cultured cells or tissues is prepared, sheared, and then denatured. Then, immunoselection and immunoprecipitation can take place using the antibody directed against 5 methylcytosine and antibody binding beads. 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', 'meta_title' => 'Methylated DNA immunoprecipitation(MeDIP) - Dna methylation | Diagenode', 'modified' => '2021-08-19 12:08:03', 'created' => '2014-09-14 05:33:34', 'ProductsApplication' => array( 'id' => '3395', 'product_id' => '2431', 'application_id' => '7' ) ) $slugs = array( (int) 0 => 'methylated-dna-immunoprecipitation' ) $applications = array( 'id' => '7', 'position' => '10', 'parent_id' => '1', 'name' => 'メチル化DNA免疫沈降', 'description' => '<div class="row"> <div class="large-12 columns">hMeDIPの場合、メチル化DNA IP（MeDIP）は5-メチルシトシン（5-mC）または5-ヒドロキシメチルシトシン（5-hmC）に対する抗体を用いたメチル化DNAのアフィニティー精製に基づきます。 <h3>使い方</h3> メチルDNA IPは以下のようにして実施されます。(要約)：培養細胞または組織からのゲノムDNAを調製、せん断、その後変性させます。 次いで、免疫選択および免疫沈降を、5メチルシトシンに対する抗体および抗体結合ビーズを用いて行うことが可能です。 単離および精製が行われた後、IP化されたメチル化DNAは、qPCR、増幅、マイクロアレイ上のハイブリダイゼーションまたは次世代シーケンシングなど、その次の分析に対応準備が完了します。 <h3>概要</h3> <p class="text-center"><img src="https://www.diagenode.com/img/applications/magnetic_medip_overview.jpg" caption="false" width="726" height="916" /></p> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'methylated-dna-immunoprecipitation', 'meta_keywords' => 'Methylated DNA immunoprecipitation,Epigenetic,DNA Methylation,qPCR,5 methylcytosine (5-mC)', 'meta_description' => 'Methylated DNA immunoprecipitation method is based on the affinity purification of methylated DNA using an antibody directed against 5 methylcytosine (5-mC). ', 'meta_title' => 'Methylated DNA immunoprecipitation(MeDIP) - Dna methylation | Diagenode', 'modified' => '2021-08-19 12:08:03', 'created' => '2014-09-14 05:33:34', 'locale' => 'jpn' ) $description = '<div class="row"> <div class="large-12 columns">hMeDIPの場合、メチル化DNA IP（MeDIP）は5-メチルシトシン（5-mC）または5-ヒドロキシメチルシトシン（5-hmC）に対する抗体を用いたメチル化DNAのアフィニティー精製に基づきます。 <h3>使い方</h3> メチルDNA IPは以下のようにして実施されます。(要約)：培養細胞または組織からのゲノムDNAを調製、せん断、その後変性させます。 次いで、免疫選択および免疫沈降を、5メチルシトシンに対する抗体および抗体結合ビーズを用いて行うことが可能です。 単離および精製が行われた後、IP化されたメチル化DNAは、qPCR、増幅、マイクロアレイ上のハイブリダイゼーションまたは次世代シーケンシングなど、その次の分析に対応準備が完了します。 <h3>概要</h3> <p class="text-center"><img src="https://www.diagenode.com/img/applications/magnetic_medip_overview.jpg" caption="false" width="726" height="916" /></p> </div> </div>' $name = 'メチル化DNA免疫沈降' $document = array( 'id' => '331', 'name' => 'Datasheet ratIgG AF-105-0025', 'description' => '<p>Datasheet description</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/C15400001-Rat-IgG.pdf', 'slug' => 'datasheet-ratigg-af-105-0025', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2017-01-30 10:20:46', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( 'id' => '902', 'product_id' => '2431', 'document_id' => '331' ) ) $sds = array( 'id' => '468', 'name' => 'Rat IgG SDS ES es', 'language' => 'es', 'url' => 'files/SDS/Rat_IgG/SDS-C15420001-Rat_IgG-ES-es-GHS_2_0.pdf', 'countries' => 'ES', 'modified' => '2020-07-01 11:03:50', 'created' => '2020-07-01 11:03:50', 'ProductsSafetySheet' => array( 'id' => '875', 'product_id' => '2431', 'safety_sheet_id' => '468' ) ) $publication = array( 'id' => '2842', 'name' => 'Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo', 'authors' => 'Komar DN, Mouriz A, Jarillo JA, Piñeiro M', 'description' => '<p>Intricate gene regulatory networks orchestrate biological processes and developmental transitions in plants. Selective transcriptional activation and silencing of genes mediate the response of plants to environmental signals and developmental cues. Therefore, insights into the mechanisms that control plant gene expression are essential to gain a deep understanding of how biological processes are regulated in plants. The chromatin immunoprecipitation (ChIP) technique described here is a procedure to identify the DNA-binding sites of proteins in genes or genomic regions of the model species Arabidopsis thaliana. The interactions with DNA of proteins of interest such as transcription factors, chromatin proteins or posttranslationally modified versions of histones can be efficiently analyzed with the ChIP protocol. This method is based on the fixation of protein-DNA interactions in vivo, random fragmentation of chromatin, immunoprecipitation of protein-DNA complexes with specific antibodies, and quantification of the DNA associated with the protein of interest by PCR techniques. The use of this methodology in Arabidopsis has contributed significantly to unveil transcriptional regulatory mechanisms that control a variety of plant biological processes. This approach allowed the identification of the binding sites of the Arabidopsis chromatin protein EBS to regulatory regions of the master gene of flowering FT. The impact of this protein in the accumulation of particular histone marks in the genomic region of FT was also revealed through ChIP analysis.</p>', 'date' => '2016-01-14', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26863263', 'doi' => '10.3791/53422', 'modified' => '2017-01-04 14:16:52', 'created' => '2016-03-09 17:05:45', 'ProductsPublication' => array( 'id' => '501', 'product_id' => '2431', 'publication_id' => '2842' ) ) $externalLink = ' <a href="http://www.ncbi.nlm.nih.gov/pubmed/26863263" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
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This Rat IgG preparation is intended for being used in </span><a href="../p/hmedip-kit-x16-monoclonal-rat-antibody-16-rxns">hMeDIP experiments (Cat. No. AF-104-0016)</a><span> as a negative control in parallel with the specific monoclonal </span><a href="../p/5-hmc-monoclonal-antibody-rat-classic-100-ug-64-ul">5-hydroxymethylcytosine antibody (Cat. No. MAb-633HMC-100)</a><span>. Both antibodies should be used at the same concentration.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15420001-hmedip.jpg" alt="hMeDIP" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. hMeDIP with the Diagenode rat IgG negative control antibody</strong><br />hMeDIP assays were performed using the Diagenode rat monoclonal antibody against 5-hmC (cat. No. C15220001) and the “hMeDIP” kit (rat, cat. No. C02010030) on 1 μg of sheared DNA from mouse ES cells. 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It contains a spectrum of the IgG subclasses present in serum of healthy animals. This Rat IgG preparation is intended for being used in </span><a href="../p/hmedip-kit-x16-monoclonal-rat-antibody-16-rxns">hMeDIP experiments (Cat. No. AF-104-0016)</a><span> as a negative control in parallel with the specific monoclonal </span><a href="../p/5-hmc-monoclonal-antibody-rat-classic-100-ug-64-ul">5-hydroxymethylcytosine antibody (Cat. No. MAb-633HMC-100)</a><span>. Both antibodies should be used at the same concentration.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15420001-hmedip.jpg" alt="hMeDIP" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. hMeDIP with the Diagenode rat IgG negative control antibody</strong><br />hMeDIP assays were performed using the Diagenode rat monoclonal antibody against 5-hmC (cat. No. C15220001) and the “hMeDIP” kit (rat, cat. No. C02010030) on 1 μg of sheared DNA from mouse ES cells. The DNA was spiked with 0.025 ng of each of the DNA standards from the 5-hmC, 5-mC & cytosine DNA standard pack (cat. No. C02040011), prior to the IP Rat IgG (cat. No. C15420001) was used as a negative IP control. 2.5 μg of antibody per ChIP experiment was used for both antibodies. Quantitative PCR was performed with the primers specific for the unmethylated, methylated and hydroxymethylated controls present in the kit. 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Then, immunoselection and immunoprecipitation can take place using the antibody directed against 5 methylcytosine and antibody binding beads. After isolation and purification is performed, the IP’d methylated DNA is ready for any subsequent analysis as qPCR, amplification, hybridization on microarrays or next generation sequencing.</p> <h2>Applications</h2> <div align="center"><a href="https://www.diagenode.com/en/p/magmedip-kit-x48-48-rxns" class="center alert radius button"> qPCR analysis</a></div> <div align="center"><a href="https://www.diagenode.com/en/p/magmedip-seq-package-V2-x10" class="center alert radius button"> NGS analysis </a></div> <h2>Advantages</h2> <ul style="font-size: 19px;" class="nobullet"> <li><i class="fa fa-arrow-circle-right"></i> <strong>Unaffected</strong> DNA</li> <li><i class="fa fa-arrow-circle-right"></i> <strong>High enrichment</strong> yield</li> <li><i class="fa fa-arrow-circle-right"></i> <strong>Robust</strong> & <strong>reproducible</strong> techniques</li> <li><i class="fa fa-arrow-circle-right"></i> <strong>NGS</strong> compatible</li> </ul> <h2></h2> </div> </div> <div id="gtx-trans" style="position: absolute; left: 17px; top: 652.938px;"> <div class="gtx-trans-icon"></div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'methylated-dna-immunoprecipitation', 'meta_keywords' => 'Methylated DNA immunoprecipitation,Epigenetic,DNA Methylation,qPCR,5 methylcytosine (5-mC)', 'meta_description' => 'Methylated DNA immunoprecipitation method is based on the affinity purification of methylated DNA using an antibody directed against 5 methylcytosine (5-mC). ', 'meta_title' => 'Methylated DNA immunoprecipitation(MeDIP) - Dna methylation | Diagenode', 'modified' => '2021-08-19 12:08:03', 'created' => '2014-09-14 05:33:34', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '22', 'position' => '40', 'parent_id' => '4', 'name' => 'Isotype controls', 'description' => '<p><span style="font-weight: 400;">Diagenode offers the negative Ctrl IgG from rabbit, rat and mouse. 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=> '10', 'parent_id' => '1', 'name' => 'Methylated DNA immunoprecipitation', 'description' => '<div class="row extra-spaced"> <div class="small-12 medium-3 large-3 columns"><center><a href="https://www.ncbi.nlm.nih.gov/pubmed/30429608" target="_blank"><img src="https://www.diagenode.com/img/banners/banner-nature-publication-580.png" /></a></center></div> <div class="small-12 medium-9 large-9 columns"> <h3>Sensitive tumour detection and classification using plasma cell-free DNA methylomes<br /><a href="https://www.ncbi.nlm.nih.gov/pubmed/30429608" target="_blank">Read the publication</a></h3> <h3 class="c-article-title u-h1" data-test="article-title" itemprop="name headline">Preparation of cfMeDIP-seq libraries for methylome profiling of plasma cell-free DNA<br /><a href="https://www.nature.com/articles/s41596-019-0202-2" target="_blank" title="cfMeDIP-seq Nature Method">Read the method</a></h3> </div> </div> <div class="row"> <div class="large-12 columns"><span>The Methylated DNA Immunoprecipitation is based on the affinity purification of methylated and hydroxymethylated DNA using, respectively, an antibody directed against 5-methylcytosine (5-mC) in the case of MeDIP or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP.</span><br /> <h2></h2> <h2>How it works</h2> <p>In brief, Methyl DNA IP is performed as follows: Genomic DNA from cultured cells or tissues is prepared, sheared, and then denatured. 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', 'meta_title' => 'Methylated DNA immunoprecipitation(MeDIP) - Dna methylation | Diagenode', 'modified' => '2021-08-19 12:08:03', 'created' => '2014-09-14 05:33:34', 'ProductsApplication' => array( 'id' => '3395', 'product_id' => '2431', 'application_id' => '7' ) ) $slugs = array( (int) 0 => 'methylated-dna-immunoprecipitation' ) $applications = array( 'id' => '7', 'position' => '10', 'parent_id' => '1', 'name' => 'メチル化DNA免疫沈降', 'description' => '<div class="row"> <div class="large-12 columns">hMeDIPの場合、メチル化DNA IP（MeDIP）は5-メチルシトシン（5-mC）または5-ヒドロキシメチルシトシン（5-hmC）に対する抗体を用いたメチル化DNAのアフィニティー精製に基づきます。 <h3>使い方</h3> メチルDNA IPは以下のようにして実施されます。(要約)：培養細胞または組織からのゲノムDNAを調製、せん断、その後変性させます。 次いで、免疫選択および免疫沈降を、5メチルシトシンに対する抗体および抗体結合ビーズを用いて行うことが可能です。 単離および精製が行われた後、IP化されたメチル化DNAは、qPCR、増幅、マイクロアレイ上のハイブリダイゼーションまたは次世代シーケンシングなど、その次の分析に対応準備が完了します。 <h3>概要</h3> <p class="text-center"><img src="https://www.diagenode.com/img/applications/magnetic_medip_overview.jpg" caption="false" width="726" height="916" /></p> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'methylated-dna-immunoprecipitation', 'meta_keywords' => 'Methylated DNA immunoprecipitation,Epigenetic,DNA Methylation,qPCR,5 methylcytosine (5-mC)', 'meta_description' => 'Methylated DNA immunoprecipitation method is based on the affinity purification of methylated DNA using an antibody directed against 5 methylcytosine (5-mC). 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It contains a spectrum of the IgG subclasses present in serum of healthy animals. This Rat IgG preparation is intended for being used in </span><a href="../p/hmedip-kit-x16-monoclonal-rat-antibody-16-rxns">hMeDIP experiments (Cat. No. AF-104-0016)</a><span> as a negative control in parallel with the specific monoclonal </span><a href="../p/5-hmc-monoclonal-antibody-rat-classic-100-ug-64-ul">5-hydroxymethylcytosine antibody (Cat. No. MAb-633HMC-100)</a><span>. Both antibodies should be used at the same concentration.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15420001-hmedip.jpg" alt="hMeDIP" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. hMeDIP with the Diagenode rat IgG negative control antibody</strong><br />hMeDIP assays were performed using the Diagenode rat monoclonal antibody against 5-hmC (cat. No. C15220001) and the “hMeDIP” kit (rat, cat. No. C02010030) on 1 μg of sheared DNA from mouse ES cells. The DNA was spiked with 0.025 ng of each of the DNA standards from the 5-hmC, 5-mC & cytosine DNA standard pack (cat. No. C02040011), prior to the IP Rat IgG (cat. No. C15420001) was used as a negative IP control. 2.5 μg of antibody per ChIP experiment was used for both antibodies. Quantitative PCR was performed with the primers specific for the unmethylated, methylated and hydroxymethylated controls present in the kit. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p> </div> </div>', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '25 µg/ 25 µl', 'catalog_number' => 'C15420001', 'old_catalog_number' => 'AF-105-0025', 'sf_code' => 'C15420001-364', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '65', 'price_USD' => '90', 'price_GBP' => '65', 'price_JPY' => '10180', 'price_CNY' => '', 'price_AUD' => '225', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'rat-igg-25-ug-25-ul', 'meta_title' => 'Rat IgG', 'meta_keywords' => '', 'meta_description' => 'Rat IgG', 'modified' => '2016-06-21 14:10:35', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '329', 'name' => 'Rat IgG', 'description' => 'The Rat IgG has been extensively validated in hydroxymethylated DNA IP (hMeDIP) assays. 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Then, immunoselection and immunoprecipitation can take place using the antibody directed against 5 methylcytosine and antibody binding beads. After isolation and purification is performed, the IP’d methylated DNA is ready for any subsequent analysis as qPCR, amplification, hybridization on microarrays or next generation sequencing.</p> <h2>Applications</h2> <div align="center"><a href="https://www.diagenode.com/en/p/magmedip-kit-x48-48-rxns" class="center alert radius button"> qPCR analysis</a></div> <div align="center"><a href="https://www.diagenode.com/en/p/magmedip-seq-package-V2-x10" class="center alert radius button"> NGS analysis </a></div> <h2>Advantages</h2> <ul style="font-size: 19px;" class="nobullet"> <li><i class="fa fa-arrow-circle-right"></i> <strong>Unaffected</strong> DNA</li> <li><i class="fa fa-arrow-circle-right"></i> <strong>High enrichment</strong> yield</li> <li><i class="fa fa-arrow-circle-right"></i> <strong>Robust</strong> & <strong>reproducible</strong> techniques</li> <li><i class="fa fa-arrow-circle-right"></i> <strong>NGS</strong> compatible</li> </ul> <h2></h2> </div> </div> <div id="gtx-trans" style="position: absolute; left: 17px; top: 652.938px;"> <div class="gtx-trans-icon"></div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'methylated-dna-immunoprecipitation', 'meta_keywords' => 'Methylated DNA immunoprecipitation,Epigenetic,DNA Methylation,qPCR,5 methylcytosine (5-mC)', 'meta_description' => 'Methylated DNA immunoprecipitation method is based on the affinity purification of methylated DNA using an antibody directed against 5 methylcytosine (5-mC). ', 'meta_title' => 'Methylated DNA immunoprecipitation(MeDIP) - Dna methylation | Diagenode', 'modified' => '2021-08-19 12:08:03', 'created' => '2014-09-14 05:33:34', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '22', 'position' => '40', 'parent_id' => '4', 'name' => 'Isotype controls', 'description' => '<p><span style="font-weight: 400;">Diagenode offers the negative Ctrl IgG from rabbit, rat and mouse. These extensively validated antibodies can be used as negative controls in ChIP, IF, hMeDIP or other experiments performed with specific antibodies made in rabbit, rat or mouse, respectively.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'isotype-controls', 'cookies_tag_id' => null, 'meta_keywords' => 'Isotype controls,DNA immunoprecipitation,Methylated DNA immunoprecipitation', 'meta_description' => 'Diagenode provides Isotype controls for Methylated DNA immunoprecipitation', 'meta_title' => 'Isotype controls for Methylated DNA immunoprecipitation | Diagenode', 'modified' => '2019-07-04 16:19:36', 'created' => '2014-09-30 14:23:34', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. 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Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '331', 'name' => 'Datasheet ratIgG AF-105-0025', 'description' => '<p>Datasheet description</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/C15400001-Rat-IgG.pdf', 'slug' => 'datasheet-ratigg-af-105-0025', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2017-01-30 10:20:46', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( 'id' => '250', 'name' => 'product/antibodies/antibody.png', 'alt' => 'Mouse IgG', 'modified' => '2020-11-27 07:00:09', 'created' => '2015-07-17 10:12:18', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '2842', 'name' => 'Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo', 'authors' => 'Komar DN, Mouriz A, Jarillo JA, Piñeiro M', 'description' => '<p>Intricate gene regulatory networks orchestrate biological processes and developmental transitions in plants. Selective transcriptional activation and silencing of genes mediate the response of plants to environmental signals and developmental cues. Therefore, insights into the mechanisms that control plant gene expression are essential to gain a deep understanding of how biological processes are regulated in plants. The chromatin immunoprecipitation (ChIP) technique described here is a procedure to identify the DNA-binding sites of proteins in genes or genomic regions of the model species Arabidopsis thaliana. The interactions with DNA of proteins of interest such as transcription factors, chromatin proteins or posttranslationally modified versions of histones can be efficiently analyzed with the ChIP protocol. This method is based on the fixation of protein-DNA interactions in vivo, random fragmentation of chromatin, immunoprecipitation of protein-DNA complexes with specific antibodies, and quantification of the DNA associated with the protein of interest by PCR techniques. 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The impact of this protein in the accumulation of particular histone marks in the genomic region of FT was also revealed through ChIP analysis.</p>', 'date' => '2016-01-14', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26863263', 'doi' => '10.3791/53422', 'modified' => '2017-01-04 14:16:52', 'created' => '2016-03-09 17:05:45', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '470', 'name' => 'Rat IgG SDS GB en', 'language' => 'en', 'url' => 'files/SDS/Rat_IgG/SDS-C15420001-Rat_IgG-GB-en-GHS_2_0.pdf', 'countries' => 'GB', 'modified' => '2020-07-01 11:04:39', 'created' => '2020-07-01 11:04:39', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '472', 'name' => 'Rat IgG SDS US en', 'language' => 'en', 'url' => 'files/SDS/Rat_IgG/SDS-C15420001-Rat_IgG-US-en-GHS_2_0.pdf', 'countries' => 'US', 'modified' => '2020-07-01 11:05:29', 'created' => 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=> '10', 'parent_id' => '1', 'name' => 'Methylated DNA immunoprecipitation', 'description' => '<div class="row extra-spaced"> <div class="small-12 medium-3 large-3 columns"><center><a href="https://www.ncbi.nlm.nih.gov/pubmed/30429608" target="_blank"><img src="https://www.diagenode.com/img/banners/banner-nature-publication-580.png" /></a></center></div> <div class="small-12 medium-9 large-9 columns"> <h3>Sensitive tumour detection and classification using plasma cell-free DNA methylomes<br /><a href="https://www.ncbi.nlm.nih.gov/pubmed/30429608" target="_blank">Read the publication</a></h3> <h3 class="c-article-title u-h1" data-test="article-title" itemprop="name headline">Preparation of cfMeDIP-seq libraries for methylome profiling of plasma cell-free DNA<br /><a href="https://www.nature.com/articles/s41596-019-0202-2" target="_blank" title="cfMeDIP-seq Nature Method">Read the method</a></h3> </div> </div> <div class="row"> <div class="large-12 columns"><span>The Methylated DNA Immunoprecipitation is based on the affinity purification of methylated and hydroxymethylated DNA using, respectively, an antibody directed against 5-methylcytosine (5-mC) in the case of MeDIP or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP.</span><br /> <h2></h2> <h2>How it works</h2> <p>In brief, Methyl DNA IP is performed as follows: Genomic DNA from cultured cells or tissues is prepared, sheared, and then denatured. Then, immunoselection and immunoprecipitation can take place using the antibody directed against 5 methylcytosine and antibody binding beads. After isolation and purification is performed, the IP’d methylated DNA is ready for any subsequent analysis as qPCR, amplification, hybridization on microarrays or next generation sequencing.</p> <h2>Applications</h2> <div align="center"><a href="https://www.diagenode.com/en/p/magmedip-kit-x48-48-rxns" class="center alert radius button"> qPCR analysis</a></div> <div align="center"><a href="https://www.diagenode.com/en/p/magmedip-seq-package-V2-x10" class="center alert radius button"> NGS analysis </a></div> <h2>Advantages</h2> <ul style="font-size: 19px;" class="nobullet"> <li><i class="fa fa-arrow-circle-right"></i> <strong>Unaffected</strong> DNA</li> <li><i class="fa fa-arrow-circle-right"></i> <strong>High enrichment</strong> yield</li> <li><i class="fa fa-arrow-circle-right"></i> <strong>Robust</strong> & <strong>reproducible</strong> techniques</li> <li><i class="fa fa-arrow-circle-right"></i> <strong>NGS</strong> compatible</li> </ul> <h2></h2> </div> </div> <div id="gtx-trans" style="position: absolute; left: 17px; top: 652.938px;"> <div class="gtx-trans-icon"></div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'methylated-dna-immunoprecipitation', 'meta_keywords' => 'Methylated DNA immunoprecipitation,Epigenetic,DNA Methylation,qPCR,5 methylcytosine (5-mC)', 'meta_description' => 'Methylated DNA immunoprecipitation method is based on the affinity purification of methylated DNA using an antibody directed against 5 methylcytosine (5-mC). ', 'meta_title' => 'Methylated DNA immunoprecipitation(MeDIP) - Dna methylation | Diagenode', 'modified' => '2021-08-19 12:08:03', 'created' => '2014-09-14 05:33:34', 'ProductsApplication' => array( 'id' => '3395', 'product_id' => '2431', 'application_id' => '7' ) ) $slugs = array( (int) 0 => 'methylated-dna-immunoprecipitation' ) $applications = array( 'id' => '7', 'position' => '10', 'parent_id' => '1', 'name' => 'メチル化DNA免疫沈降', 'description' => '<div class="row"> <div class="large-12 columns">hMeDIPの場合、メチル化DNA IP（MeDIP）は5-メチルシトシン（5-mC）または5-ヒドロキシメチルシトシン（5-hmC）に対する抗体を用いたメチル化DNAのアフィニティー精製に基づきます。 <h3>使い方</h3> メチルDNA IPは以下のようにして実施されます。(要約)：培養細胞または組織からのゲノムDNAを調製、せん断、その後変性させます。 次いで、免疫選択および免疫沈降を、5メチルシトシンに対する抗体および抗体結合ビーズを用いて行うことが可能です。 単離および精製が行われた後、IP化されたメチル化DNAは、qPCR、増幅、マイクロアレイ上のハイブリダイゼーションまたは次世代シーケンシングなど、その次の分析に対応準備が完了します。 <h3>概要</h3> <p class="text-center"><img src="https://www.diagenode.com/img/applications/magnetic_medip_overview.jpg" caption="false" width="726" height="916" /></p> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'methylated-dna-immunoprecipitation', 'meta_keywords' => 'Methylated DNA immunoprecipitation,Epigenetic,DNA Methylation,qPCR,5 methylcytosine (5-mC)', 'meta_description' => 'Methylated DNA immunoprecipitation method is based on the affinity purification of methylated DNA using an antibody directed against 5 methylcytosine (5-mC). 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Selective transcriptional activation and silencing of genes mediate the response of plants to environmental signals and developmental cues. Therefore, insights into the mechanisms that control plant gene expression are essential to gain a deep understanding of how biological processes are regulated in plants. The chromatin immunoprecipitation (ChIP) technique described here is a procedure to identify the DNA-binding sites of proteins in genes or genomic regions of the model species Arabidopsis thaliana. The interactions with DNA of proteins of interest such as transcription factors, chromatin proteins or posttranslationally modified versions of histones can be efficiently analyzed with the ChIP protocol. This method is based on the fixation of protein-DNA interactions in vivo, random fragmentation of chromatin, immunoprecipitation of protein-DNA complexes with specific antibodies, and quantification of the DNA associated with the protein of interest by PCR techniques. The use of this methodology in Arabidopsis has contributed significantly to unveil transcriptional regulatory mechanisms that control a variety of plant biological processes. This approach allowed the identification of the binding sites of the Arabidopsis chromatin protein EBS to regulatory regions of the master gene of flowering FT. The impact of this protein in the accumulation of particular histone marks in the genomic region of FT was also revealed through ChIP analysis.</p>', 'date' => '2016-01-14', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26863263', 'doi' => '10.3791/53422', 'modified' => '2017-01-04 14:16:52', 'created' => '2016-03-09 17:05:45', 'ProductsPublication' => array( 'id' => '501', 'product_id' => '2431', 'publication_id' => '2842' ) ) $externalLink = ' <a href="http://www.ncbi.nlm.nih.gov/pubmed/26863263" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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It contains a spectrum of the IgG subclasses present in serum of healthy animals. This Rat IgG preparation is intended for being used in </span><a href="../p/hmedip-kit-x16-monoclonal-rat-antibody-16-rxns">hMeDIP experiments (Cat. No. AF-104-0016)</a><span> as a negative control in parallel with the specific monoclonal </span><a href="../p/5-hmc-monoclonal-antibody-rat-classic-100-ug-64-ul">5-hydroxymethylcytosine antibody (Cat. No. MAb-633HMC-100)</a><span>. Both antibodies should be used at the same concentration.</span></p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/c15420001-hmedip.jpg" alt="hMeDIP" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. hMeDIP with the Diagenode rat IgG negative control antibody</strong><br />hMeDIP assays were performed using the Diagenode rat monoclonal antibody against 5-hmC (cat. No. C15220001) and the “hMeDIP” kit (rat, cat. No. C02010030) on 1 μg of sheared DNA from mouse ES cells. The DNA was spiked with 0.025 ng of each of the DNA standards from the 5-hmC, 5-mC & cytosine DNA standard pack (cat. No. C02040011), prior to the IP Rat IgG (cat. No. C15420001) was used as a negative IP control. 2.5 μg of antibody per ChIP experiment was used for both antibodies. Quantitative PCR was performed with the primers specific for the unmethylated, methylated and hydroxymethylated controls present in the kit. 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Then, immunoselection and immunoprecipitation can take place using the antibody directed against 5 methylcytosine and antibody binding beads. 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=> '10', 'parent_id' => '1', 'name' => 'Methylated DNA immunoprecipitation', 'description' => '<div class="row extra-spaced"> <div class="small-12 medium-3 large-3 columns"><center><a href="https://www.ncbi.nlm.nih.gov/pubmed/30429608" target="_blank"><img src="https://www.diagenode.com/img/banners/banner-nature-publication-580.png" /></a></center></div> <div class="small-12 medium-9 large-9 columns"> <h3>Sensitive tumour detection and classification using plasma cell-free DNA methylomes<br /><a href="https://www.ncbi.nlm.nih.gov/pubmed/30429608" target="_blank">Read the publication</a></h3> <h3 class="c-article-title u-h1" data-test="article-title" itemprop="name headline">Preparation of cfMeDIP-seq libraries for methylome profiling of plasma cell-free DNA<br /><a href="https://www.nature.com/articles/s41596-019-0202-2" target="_blank" title="cfMeDIP-seq Nature Method">Read the method</a></h3> </div> </div> <div class="row"> <div class="large-12 columns"><span>The Methylated DNA Immunoprecipitation is based on the affinity purification of methylated and hydroxymethylated DNA using, respectively, an antibody directed against 5-methylcytosine (5-mC) in the case of MeDIP or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP.</span><br /> <h2></h2> <h2>How it works</h2> <p>In brief, Methyl DNA IP is performed as follows: Genomic DNA from cultured cells or tissues is prepared, sheared, and then denatured. Then, immunoselection and immunoprecipitation can take place using the antibody directed against 5 methylcytosine and antibody binding beads. After isolation and purification is performed, the IP’d methylated DNA is ready for any subsequent analysis as qPCR, amplification, hybridization on microarrays or next generation sequencing.</p> <h2>Applications</h2> <div align="center"><a href="https://www.diagenode.com/en/p/magmedip-kit-x48-48-rxns" class="center alert radius button"> qPCR analysis</a></div> <div align="center"><a href="https://www.diagenode.com/en/p/magmedip-seq-package-V2-x10" class="center alert radius button"> NGS analysis </a></div> <h2>Advantages</h2> <ul style="font-size: 19px;" class="nobullet"> <li><i class="fa fa-arrow-circle-right"></i> <strong>Unaffected</strong> DNA</li> <li><i class="fa fa-arrow-circle-right"></i> <strong>High enrichment</strong> yield</li> <li><i class="fa fa-arrow-circle-right"></i> <strong>Robust</strong> & <strong>reproducible</strong> techniques</li> <li><i class="fa fa-arrow-circle-right"></i> <strong>NGS</strong> compatible</li> </ul> <h2></h2> </div> </div> <div id="gtx-trans" style="position: absolute; left: 17px; top: 652.938px;"> <div class="gtx-trans-icon"></div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'methylated-dna-immunoprecipitation', 'meta_keywords' => 'Methylated DNA immunoprecipitation,Epigenetic,DNA Methylation,qPCR,5 methylcytosine (5-mC)', 'meta_description' => 'Methylated DNA immunoprecipitation method is based on the affinity purification of methylated DNA using an antibody directed against 5 methylcytosine (5-mC). ', 'meta_title' => 'Methylated DNA immunoprecipitation(MeDIP) - Dna methylation | Diagenode', 'modified' => '2021-08-19 12:08:03', 'created' => '2014-09-14 05:33:34', 'ProductsApplication' => array( 'id' => '3395', 'product_id' => '2431', 'application_id' => '7' ) ) $slugs = array( (int) 0 => 'methylated-dna-immunoprecipitation' ) $applications = array( 'id' => '7', 'position' => '10', 'parent_id' => '1', 'name' => 'メチル化DNA免疫沈降', 'description' => '<div class="row"> <div class="large-12 columns">hMeDIPの場合、メチル化DNA IP（MeDIP）は5-メチルシトシン（5-mC）または5-ヒドロキシメチルシトシン（5-hmC）に対する抗体を用いたメチル化DNAのアフィニティー精製に基づきます。 <h3>使い方</h3> メチルDNA IPは以下のようにして実施されます。(要約)：培養細胞または組織からのゲノムDNAを調製、せん断、その後変性させます。 次いで、免疫選択および免疫沈降を、5メチルシトシンに対する抗体および抗体結合ビーズを用いて行うことが可能です。 単離および精製が行われた後、IP化されたメチル化DNAは、qPCR、増幅、マイクロアレイ上のハイブリダイゼーションまたは次世代シーケンシングなど、その次の分析に対応準備が完了します。 <h3>概要</h3> <p class="text-center"><img src="https://www.diagenode.com/img/applications/magnetic_medip_overview.jpg" caption="false" width="726" height="916" /></p> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'methylated-dna-immunoprecipitation', 'meta_keywords' => 'Methylated DNA immunoprecipitation,Epigenetic,DNA Methylation,qPCR,5 methylcytosine (5-mC)', 'meta_description' => 'Methylated DNA immunoprecipitation method is based on the affinity purification of methylated DNA using an antibody directed against 5 methylcytosine (5-mC). 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Selective transcriptional activation and silencing of genes mediate the response of plants to environmental signals and developmental cues. Therefore, insights into the mechanisms that control plant gene expression are essential to gain a deep understanding of how biological processes are regulated in plants. The chromatin immunoprecipitation (ChIP) technique described here is a procedure to identify the DNA-binding sites of proteins in genes or genomic regions of the model species Arabidopsis thaliana. The interactions with DNA of proteins of interest such as transcription factors, chromatin proteins or posttranslationally modified versions of histones can be efficiently analyzed with the ChIP protocol. This method is based on the fixation of protein-DNA interactions in vivo, random fragmentation of chromatin, immunoprecipitation of protein-DNA complexes with specific antibodies, and quantification of the DNA associated with the protein of interest by PCR techniques. 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The impact of this protein in the accumulation of particular histone marks in the genomic region of FT was also revealed through ChIP analysis.</p>', 'date' => '2016-01-14', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/26863263', 'doi' => '10.3791/53422', 'modified' => '2017-01-04 14:16:52', 'created' => '2016-03-09 17:05:45', 'ProductsPublication' => array( 'id' => '501', 'product_id' => '2431', 'publication_id' => '2842' ) ) $externalLink = ' <a href="http://www.ncbi.nlm.nih.gov/pubmed/26863263" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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