The Rat IgG has been extensively validated in hydroxymethylated DNA IP (hMeDIP) assays. It contains a spectrum of the IgG subclasses present in serum of healthy animals. This Rat IgG preparation is intended for being used in hMeDIP experiments (Cat. No. AF-104-0016) as a negative control in parallel with the specific monoclonal 5-hydroxymethylcytosine antibody (Cat. No. MAb-633HMC-100). Both antibodies should be used at the same concentration.
| Lot | D001 |
|---|---|
| Concentration | 1.0 µg/µl |
| Species reactivity | none |
| Type | Polyclonal |
| Purity | Protein A purified |
| Host | Rat |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Figure 1. hMeDIP with the Diagenode rat IgG negative control antibody
hMeDIP assays were performed using the Diagenode rat monoclonal antibody against 5-hmC (cat. No. C15220001) and the “hMeDIP” kit (rat, cat. No. C02010030) on 1 μg of sheared DNA from mouse ES cells. The DNA was spiked with 0.025 ng of each of the DNA standards from the 5-hmC, 5-mC & cytosine DNA standard pack (cat. No. C02040011), prior to the IP Rat IgG (cat. No. C15420001) was used as a negative IP control. 2.5 μg of antibody per ChIP experiment was used for both antibodies. Quantitative PCR was performed with the primers specific for the unmethylated, methylated and hydroxymethylated controls present in the kit. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
 How to properly cite our product/service in your workWe strongly recommend using this: Rat IgG (Hologic Diagenode Cat# C15420001 Lot# D001). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo |
