| ChIP-qPCR (ab) Read more |
Polyclonal antibody raised in rabbit against RFXAP (Regulatory factor X-associated protein), using the recombinant protein.
| Lot | 001 |
|---|---|
| Concentration | 1.0 µg/µl |
| Species reactivity | Human |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP * | 5-7 μg/IP | Fig 1 |
* Please note that of the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 μg per IP.
Figure 1 ChIP results obtained with the Diagenode antibody directed against RFXAP
ChIP assays were performed using NALM cells (a cell line derived from human pre-B leukemia), the Diagenode antibody against RFXAP (cat. No. pAb-061-050) and optimized primer sets for PCR. Sheared chromatin from 2 million cells and respectively 5 and 7 μg of antibody were used per ChIP experiment. An anti-flag antibody (lane 2) was used as negative IP control. Figure 1 shows the result of the end-point PCR with primers for HLA_DRA, used as positive control (lower panel) and for HLA_DOB, used a negative PCR control (upper panel).
 How to properly cite our product/service in your workWe strongly recommend using this: RFX-AP polyclonal antibody (Hologic Diagenode Cat# C15410061 Lot# 001). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |