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Rice seedlings OsChr4-reg9 primer pair

Catalog Number

Format

Price

C17040008-500

500 µl

$100.00

Other format

C17040008-50

50 µl

This primer pair specifically amplifies a genomic region from chromosome 4 from Rice (Oriza sativa ssp Japonica cv. Nipponbare). The primers are thoroughly tested in conjunction with the Universal Plant ChIP-seq kit and optimized for routine SYBR® Green Real-Time qPCR assay following ChIP.

資料
Rice seedlings OsChr4-reg9 Primer pair (C17040008) DATASHEET
Datasheet Download

関連商品
- C01010152
  
  Universal Plant ChIP-seq kit
- C01010153
  
  Auto Universal Plant ChIP-seq kit

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<p style="text-align: justify;">The <strong>Universal Plant ChIP-seq kit</strong> offers the convenience of extracting plant chromatin from a wide variety of plants including&nbsp;&nbsp;Arabidopsis, maize, rice, tomato and poplar. This complete kit has been specifically optimized for <strong>plant chromatin extraction</strong> and includes reagents for chromatin preparation, immunoprecipitation, plant-specific control primer pairs, control antibody, and DNA purification.</p>',
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<h3>Successful ChIP-seq experiments for a variety of plants</h3>
<div class="row">
<div class="small-6 columns">
<h4 class="text-center">Arabidopsis</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG1"> <img src="https://www.diagenode.com/img/landing-pages/Plant-ChIP-figure-3-A-small.jpg" /> </a></p>
<div id="IMG1" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/Plant-ChIP-figure-3-A.png" /></p>
<a class="close-reveal-modal" aria-label="Close">&times;</a></div>
<p><small><strong>Figure 1.</strong> ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation&trade; kit</a> from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.</small></p>
</div>
<div class="small-6 columns">
<h4 class="text-center">Poplar</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG2"><img src="https://www.diagenode.com/img/landing-pages/poplar-small.jpg" /> </a></p>
<div id="IMG2" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/poplar.jpg" /></p>
<a class="close-reveal-modal" aria-label="Close">&times;</a></div>
<p><small><strong>Figure 3.</strong> ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation&trade; kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<h4 class="text-center">Tomato</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG3"> <img src="https://www.diagenode.com/img/landing-pages/tomtato-small.jpg" /> </a></p>
<div id="IMG3" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></p>
<a class="close-reveal-modal" aria-label="Close">&times;</a></div>
<p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation&trade; kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p>
</div>
<div class="small-6 columns">
<h4 class="text-center">Maize</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG4"> <img src="https://www.diagenode.com/img/landing-pages/maize-small.jpg" /> </a></p>
<div id="IMG4" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></p>
<a class="close-reveal-modal" aria-label="Close">&times;</a></div>
<p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our <a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">Premium H3K27me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation&trade; kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<p><strong>&nbsp;</strong></p>
<table style="width: 856px;">
<tbody>
<tr>
<td style="width: 224px;">
<h4><strong>Plant Species</strong></h4>
</td>
<td style="width: 341px;">
<h4><strong>Validated antibodies</strong></h4>
</td>
<td style="width: 357px;">
<h4><strong>Validated primer pairs</strong></h4>
</td>
</tr>
<tr>
<td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrMYBTF1-primer-pair-50ul">Poplar xylem PtrMYBTF1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
</tbody>
</table>
<p><strong></strong></p>
<p><strong></strong></p>',
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			'slug' => 'universal-plant-chip-seq-kit-x24-24-rxns',
			'meta_title' => 'Universal Plant ChIP-seq kit | Diagenode',
			'meta_keywords' => 'plant epigenetics, plant ChIP, plant ChIP-seq, Arabidopsis, maize, rice, tomato, poplar',
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			'name' => 'Auto Universal Plant ChIP-seq kit',
			'description' => '<p style="text-align: justify;">The <strong>Auto Universal Plant ChIP-seq</strong> kit offers the convenience of extracting plant chromatin from a wide variety of plants including&nbsp;&nbsp;Arabidopsis, maize, rice, tomato and poplar and has been validated for the <strong>IP-Star&reg; automated system</strong>. This complete kit has been specifically optimized for <strong>plant chromatin extraction</strong> and includes reagents for chromatin preparation, immunoprecipitation, plant-specific control primer pairs, control antibody, and DNA purification.</p>',
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<ul>
<li><strong>Universal compatiblity</strong> with a wide variety of plant species</li>
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<h3>Successful ChIP-seq experiments for a variety of plants</h3>
<div class="row">
<div class="small-6 columns"><center>Arabidopsis</center><center><img src="https://www.diagenode.com/img/landing-pages/Plant-ChIP-figure-3-A.png" /></center>
<p><small><strong>Figure 1.</strong> ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation&trade; kit</a> from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.</small></p>
</div>
<div class="small-6 columns"><center>Poplar</center><center><img src="https://www.diagenode.com/img/landing-pages/poplar.jpg" /></center>
<p><small><strong>Figure 3.</strong> ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation&trade; kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns"><center>Tomato</center><center><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></center>
<p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our Premium H3K4me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation&trade; kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p>
</div>
<div class="small-6 columns"><center>Maize</center><center><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></center>
<p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our Premium H3K27me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation&trade; kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<table style="width: 856px;">
<tbody>
<tr>
<td style="width: 224px;">
<h4><strong>Plant Species</strong></h4>
</td>
<td style="width: 341px;">
<h4><strong>Validated antibodies</strong></h4>
</td>
<td style="width: 357px;">
<h4><strong>Validated primer pairs</strong></h4>
</td>
</tr>
<tr>
<td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrMYBTF1-primer-pair-50ul">Poplar xylem PtrMYBTF1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
</tbody>
</table>',
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			'price_AUD' => '1438',
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			'slug' => 'auto-universal-plant-chip-seq-kit-x24-24-rxns',
			'meta_title' => 'Auto Universal Plant ChIP-seq kit | Diagenode',
			'meta_keywords' => 'plant epigenetics, plant ChIP, plant ChIP-seq, Arabidopsis, maize, rice, tomato, poplar, automated system, automation, IP-Star',
			'meta_description' => 'Plant chromatin extraction from Arabidopsis,maize,rice,tomato,poplar.Complete ChIP kit including plant-specific primer pairs,antibody.Compatible with Automation',
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			'name' => 'ChIP-qPCR',
			'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns text-justify">
<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong>&nbsp;against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
<li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li>
</ol>
</div>
</div>
<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div>
</div>
</div>
</div>
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			'meta_keywords' => 'Chromatin immunoprecipitation,ChIP Quantitative PCR,polymerase chain reaction (PCR)',
			'meta_description' => 'Diagenode's ChIP qPCR kits can be used to quantify enriched DNA after chromatin immunoprecipitation. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of',
			'meta_title' => 'ChIP Quantitative PCR (ChIP-qPCR) | Diagenode',
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<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
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<div class="small-6 columns"><center>Tomato</center><center><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></center>
<p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our Premium H3K4me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation&trade; kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p>
</div>
<div class="small-6 columns"><center>Maize</center><center><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></center>
<p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our Premium H3K27me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation&trade; kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<table style="width: 856px;">
<tbody>
<tr>
<td style="width: 224px;">
<h4><strong>Plant Species</strong></h4>
</td>
<td style="width: 341px;">
<h4><strong>Validated antibodies</strong></h4>
</td>
<td style="width: 357px;">
<h4><strong>Validated primer pairs</strong></h4>
</td>
</tr>
<tr>
<td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrMYBTF1-primer-pair-50ul">Poplar xylem PtrMYBTF1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
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<p style="text-align: justify;">The <strong>Universal Plant ChIP-seq kit</strong> offers the convenience of extracting plant chromatin from a wide variety of plants including&nbsp;&nbsp;Arabidopsis, maize, rice, tomato and poplar. This complete kit has been specifically optimized for <strong>plant chromatin extraction</strong> and includes reagents for chromatin preparation, immunoprecipitation, plant-specific control primer pairs, control antibody, and DNA purification.</p>',
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<h3>Successful ChIP-seq experiments for a variety of plants</h3>
<div class="row">
<div class="small-6 columns">
<h4 class="text-center">Arabidopsis</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG1"> <img src="https://www.diagenode.com/img/landing-pages/Plant-ChIP-figure-3-A-small.jpg" /> </a></p>
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<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/Plant-ChIP-figure-3-A.png" /></p>
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<p><small><strong>Figure 1.</strong> ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation&trade; kit</a> from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.</small></p>
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<div class="small-6 columns">
<h4 class="text-center">Poplar</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG2"><img src="https://www.diagenode.com/img/landing-pages/poplar-small.jpg" /> </a></p>
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<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/poplar.jpg" /></p>
<a class="close-reveal-modal" aria-label="Close">&times;</a></div>
<p><small><strong>Figure 3.</strong> ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation&trade; kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<h4 class="text-center">Tomato</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG3"> <img src="https://www.diagenode.com/img/landing-pages/tomtato-small.jpg" /> </a></p>
<div id="IMG3" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></p>
<a class="close-reveal-modal" aria-label="Close">&times;</a></div>
<p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation&trade; kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p>
</div>
<div class="small-6 columns">
<h4 class="text-center">Maize</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG4"> <img src="https://www.diagenode.com/img/landing-pages/maize-small.jpg" /> </a></p>
<div id="IMG4" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></p>
<a class="close-reveal-modal" aria-label="Close">&times;</a></div>
<p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our <a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">Premium H3K27me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation&trade; kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<p><strong>&nbsp;</strong></p>
<table style="width: 856px;">
<tbody>
<tr>
<td style="width: 224px;">
<h4><strong>Plant Species</strong></h4>
</td>
<td style="width: 341px;">
<h4><strong>Validated antibodies</strong></h4>
</td>
<td style="width: 357px;">
<h4><strong>Validated primer pairs</strong></h4>
</td>
</tr>
<tr>
<td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrMYBTF1-primer-pair-50ul">Poplar xylem PtrMYBTF1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
</tbody>
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<div class="small-6 columns"><center>Arabidopsis</center><center><img src="https://www.diagenode.com/img/landing-pages/Plant-ChIP-figure-3-A.png" /></center>
<p><small><strong>Figure 1.</strong> ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation&trade; kit</a> from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.</small></p>
</div>
<div class="small-6 columns"><center>Poplar</center><center><img src="https://www.diagenode.com/img/landing-pages/poplar.jpg" /></center>
<p><small><strong>Figure 3.</strong> ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation&trade; kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns"><center>Tomato</center><center><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></center>
<p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our Premium H3K4me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation&trade; kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p>
</div>
<div class="small-6 columns"><center>Maize</center><center><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></center>
<p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our Premium H3K27me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation&trade; kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<table style="width: 856px;">
<tbody>
<tr>
<td style="width: 224px;">
<h4><strong>Plant Species</strong></h4>
</td>
<td style="width: 341px;">
<h4><strong>Validated antibodies</strong></h4>
</td>
<td style="width: 357px;">
<h4><strong>Validated primer pairs</strong></h4>
</td>
</tr>
<tr>
<td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrMYBTF1-primer-pair-50ul">Poplar xylem PtrMYBTF1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
</tbody>
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<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
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<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong>&nbsp;against the histone or transcription factor of interest</li>
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<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
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<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
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<td style="width: 341px;">
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<td style="width: 357px;">
<h4><strong>Validated primer pairs</strong></h4>
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<td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td>
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<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td>
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<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td>
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<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
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<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
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<td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td>
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<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td>
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<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
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<tr>
<td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td>
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<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td>
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<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
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<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td>
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<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td>
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<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
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<td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td>
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<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrMYBTF1-primer-pair-50ul">Poplar xylem PtrMYBTF1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
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<div id="IMG1" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/Plant-ChIP-figure-3-A.png" /></p>
<a class="close-reveal-modal" aria-label="Close">&times;</a></div>
<p><small><strong>Figure 1.</strong> ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation&trade; kit</a> from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.</small></p>
</div>
<div class="small-6 columns">
<h4 class="text-center">Poplar</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG2"><img src="https://www.diagenode.com/img/landing-pages/poplar-small.jpg" /> </a></p>
<div id="IMG2" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/poplar.jpg" /></p>
<a class="close-reveal-modal" aria-label="Close">&times;</a></div>
<p><small><strong>Figure 3.</strong> ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation&trade; kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<h4 class="text-center">Tomato</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG3"> <img src="https://www.diagenode.com/img/landing-pages/tomtato-small.jpg" /> </a></p>
<div id="IMG3" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></p>
<a class="close-reveal-modal" aria-label="Close">&times;</a></div>
<p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation&trade; kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p>
</div>
<div class="small-6 columns">
<h4 class="text-center">Maize</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG4"> <img src="https://www.diagenode.com/img/landing-pages/maize-small.jpg" /> </a></p>
<div id="IMG4" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></p>
<a class="close-reveal-modal" aria-label="Close">&times;</a></div>
<p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our <a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">Premium H3K27me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation&trade; kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<p><strong>&nbsp;</strong></p>
<table style="width: 856px;">
<tbody>
<tr>
<td style="width: 224px;">
<h4><strong>Plant Species</strong></h4>
</td>
<td style="width: 341px;">
<h4><strong>Validated antibodies</strong></h4>
</td>
<td style="width: 357px;">
<h4><strong>Validated primer pairs</strong></h4>
</td>
</tr>
<tr>
<td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrMYBTF1-primer-pair-50ul">Poplar xylem PtrMYBTF1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
</tbody>
</table>
<p><strong></strong></p>
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			'slug' => 'universal-plant-chip-seq-kit-x24-24-rxns',
			'meta_title' => 'Universal Plant ChIP-seq kit | Diagenode',
			'meta_keywords' => 'plant epigenetics, plant ChIP, plant ChIP-seq, Arabidopsis, maize, rice, tomato, poplar',
			'meta_description' => 'Optimized extraction of plant chromatin from Arabidopsis,maize,rice,tomato,poplar.Complete ChIP kit including plant-specific control primer pairs and antibody',
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			'name' => 'Auto Universal Plant ChIP-seq kit',
			'description' => '<p style="text-align: justify;">The <strong>Auto Universal Plant ChIP-seq</strong> kit offers the convenience of extracting plant chromatin from a wide variety of plants including&nbsp;&nbsp;Arabidopsis, maize, rice, tomato and poplar and has been validated for the <strong>IP-Star&reg; automated system</strong>. This complete kit has been specifically optimized for <strong>plant chromatin extraction</strong> and includes reagents for chromatin preparation, immunoprecipitation, plant-specific control primer pairs, control antibody, and DNA purification.</p>',
			'label1' => 'Characteristics',
			'info1' => '<p></p>
<ul>
<li><strong>Universal compatiblity</strong> with a wide variety of plant species</li>
<li>Optimized and <strong>complete kit</strong> for start-to-finish plant ChIP</li>
<li>Validated for the&nbsp;high throughput <strong>IP-Star&reg; Automated System</strong></li>
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<h3>Successful ChIP-seq experiments for a variety of plants</h3>
<div class="row">
<div class="small-6 columns"><center>Arabidopsis</center><center><img src="https://www.diagenode.com/img/landing-pages/Plant-ChIP-figure-3-A.png" /></center>
<p><small><strong>Figure 1.</strong> ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation&trade; kit</a> from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.</small></p>
</div>
<div class="small-6 columns"><center>Poplar</center><center><img src="https://www.diagenode.com/img/landing-pages/poplar.jpg" /></center>
<p><small><strong>Figure 3.</strong> ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation&trade; kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns"><center>Tomato</center><center><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></center>
<p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our Premium H3K4me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation&trade; kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p>
</div>
<div class="small-6 columns"><center>Maize</center><center><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></center>
<p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our Premium H3K27me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation&trade; kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<table style="width: 856px;">
<tbody>
<tr>
<td style="width: 224px;">
<h4><strong>Plant Species</strong></h4>
</td>
<td style="width: 341px;">
<h4><strong>Validated antibodies</strong></h4>
</td>
<td style="width: 357px;">
<h4><strong>Validated primer pairs</strong></h4>
</td>
</tr>
<tr>
<td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
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<tr>
<td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
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<tr>
<td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td>
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<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
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<td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrMYBTF1-primer-pair-50ul">Poplar xylem PtrMYBTF1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
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<div class="small-6 columns"><center>Maize</center><center><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></center>
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<td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td>
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<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td>
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<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td>
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<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
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<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
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<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
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<td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td>
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<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td>
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<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
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<tr>
<td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td>
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<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
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<tr>
<td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td>
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<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrMYBTF1-primer-pair-50ul">Poplar xylem PtrMYBTF1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
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<p style="text-align: justify;">The <strong>Universal Plant ChIP-seq kit</strong> offers the convenience of extracting plant chromatin from a wide variety of plants including&nbsp;&nbsp;Arabidopsis, maize, rice, tomato and poplar. This complete kit has been specifically optimized for <strong>plant chromatin extraction</strong> and includes reagents for chromatin preparation, immunoprecipitation, plant-specific control primer pairs, control antibody, and DNA purification.</p>',
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<h3>Successful ChIP-seq experiments for a variety of plants</h3>
<div class="row">
<div class="small-6 columns">
<h4 class="text-center">Arabidopsis</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG1"> <img src="https://www.diagenode.com/img/landing-pages/Plant-ChIP-figure-3-A-small.jpg" /> </a></p>
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<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/Plant-ChIP-figure-3-A.png" /></p>
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<p><small><strong>Figure 1.</strong> ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation&trade; kit</a> from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.</small></p>
</div>
<div class="small-6 columns">
<h4 class="text-center">Poplar</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG2"><img src="https://www.diagenode.com/img/landing-pages/poplar-small.jpg" /> </a></p>
<div id="IMG2" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/poplar.jpg" /></p>
<a class="close-reveal-modal" aria-label="Close">&times;</a></div>
<p><small><strong>Figure 3.</strong> ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation&trade; kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<h4 class="text-center">Tomato</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG3"> <img src="https://www.diagenode.com/img/landing-pages/tomtato-small.jpg" /> </a></p>
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<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></p>
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<p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our <a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation&trade; kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p>
</div>
<div class="small-6 columns">
<h4 class="text-center">Maize</h4>
<p class="text-center"><a href="#" data-reveal-id="IMG4"> <img src="https://www.diagenode.com/img/landing-pages/maize-small.jpg" /> </a></p>
<div id="IMG4" class="reveal-modal" data-reveal="" aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<p class="text-center"><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></p>
<a class="close-reveal-modal" aria-label="Close">&times;</a></div>
<p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our <a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-50-mg-27-ml">Premium H3K27me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation&trade; kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<p><strong>&nbsp;</strong></p>
<table style="width: 856px;">
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<td style="width: 224px;">
<h4><strong>Plant Species</strong></h4>
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<td style="width: 341px;">
<h4><strong>Validated antibodies</strong></h4>
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<td style="width: 357px;">
<h4><strong>Validated primer pairs</strong></h4>
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<td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td>
</tr>
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<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td>
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<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
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<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
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<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
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<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrMYBTF1-primer-pair-50ul">Poplar xylem PtrMYBTF1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
</tbody>
</table>
<p><strong></strong></p>
<p><strong></strong></p>',
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			'slug' => 'universal-plant-chip-seq-kit-x24-24-rxns',
			'meta_title' => 'Universal Plant ChIP-seq kit | Diagenode',
			'meta_keywords' => 'plant epigenetics, plant ChIP, plant ChIP-seq, Arabidopsis, maize, rice, tomato, poplar',
			'meta_description' => 'Optimized extraction of plant chromatin from Arabidopsis,maize,rice,tomato,poplar.Complete ChIP kit including plant-specific control primer pairs and antibody',
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			'name' => 'Auto Universal Plant ChIP-seq kit',
			'description' => '<p style="text-align: justify;">The <strong>Auto Universal Plant ChIP-seq</strong> kit offers the convenience of extracting plant chromatin from a wide variety of plants including&nbsp;&nbsp;Arabidopsis, maize, rice, tomato and poplar and has been validated for the <strong>IP-Star&reg; automated system</strong>. This complete kit has been specifically optimized for <strong>plant chromatin extraction</strong> and includes reagents for chromatin preparation, immunoprecipitation, plant-specific control primer pairs, control antibody, and DNA purification.</p>',
			'label1' => 'Characteristics',
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<ul>
<li><strong>Universal compatiblity</strong> with a wide variety of plant species</li>
<li>Optimized and <strong>complete kit</strong> for start-to-finish plant ChIP</li>
<li>Validated for the&nbsp;high throughput <strong>IP-Star&reg; Automated System</strong></li>
</ul>
<h3>Successful ChIP-seq experiments for a variety of plants</h3>
<div class="row">
<div class="small-6 columns"><center>Arabidopsis</center><center><img src="https://www.diagenode.com/img/landing-pages/Plant-ChIP-figure-3-A.png" /></center>
<p><small><strong>Figure 1.</strong> ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation&trade; kit</a> from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.</small></p>
</div>
<div class="small-6 columns"><center>Poplar</center><center><img src="https://www.diagenode.com/img/landing-pages/poplar.jpg" /></center>
<p><small><strong>Figure 3.</strong> ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation&trade; kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns"><center>Tomato</center><center><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></center>
<p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our Premium H3K4me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation&trade; kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p>
</div>
<div class="small-6 columns"><center>Maize</center><center><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></center>
<p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our Premium H3K27me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation&trade; kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<table style="width: 856px;">
<tbody>
<tr>
<td style="width: 224px;">
<h4><strong>Plant Species</strong></h4>
</td>
<td style="width: 341px;">
<h4><strong>Validated antibodies</strong></h4>
</td>
<td style="width: 357px;">
<h4><strong>Validated primer pairs</strong></h4>
</td>
</tr>
<tr>
<td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrMYBTF1-primer-pair-50ul">Poplar xylem PtrMYBTF1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
</tbody>
</table>',
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			'sf_code' => 'C01010153-',
			'type' => 'RFR',
			'search_order' => '04-undefined',
			'price_EUR' => '575',
			'price_USD' => '575',
			'price_GBP' => '525',
			'price_JPY' => '90075',
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			'price_AUD' => '1438',
			'country' => 'ALL',
			'except_countries' => 'Japan',
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			'slug' => 'auto-universal-plant-chip-seq-kit-x24-24-rxns',
			'meta_title' => 'Auto Universal Plant ChIP-seq kit | Diagenode',
			'meta_keywords' => 'plant epigenetics, plant ChIP, plant ChIP-seq, Arabidopsis, maize, rice, tomato, poplar, automated system, automation, IP-Star',
			'meta_description' => 'Plant chromatin extraction from Arabidopsis,maize,rice,tomato,poplar.Complete ChIP kit including plant-specific primer pairs,antibody.Compatible with Automation',
			'modified' => '2017-08-19 10:28:51',
			'created' => '2016-02-22 15:08:26',
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	'Application' => array(
		(int) 0 => array(
			'id' => '10',
			'position' => '10',
			'parent_id' => '2',
			'name' => 'ChIP-qPCR',
			'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns text-justify">
<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
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<h3>Successful ChIP-seq experiments for a variety of plants</h3>
<div class="row">
<div class="small-6 columns"><center>Arabidopsis</center><center><img src="https://www.diagenode.com/img/landing-pages/Plant-ChIP-figure-3-A.png" /></center>
<p><small><strong>Figure 1.</strong> ChIP-seq was performed on Arabidopsis thaliana (Col-0) seedlings using our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">Premium H3K4me3 ChIP-seq grade antibody</a>. Libraries were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation&trade; kit</a> from 1 ng (green), 500 pg (orange) and 100 pg (red) IP'd DNA and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in blue represents a public dataset (NCBI GEO Dataset GSM1193621) that we used as an external reference. Enrichments along a wide region of chromosome 5 are uniform regardless of the starting material amount for the preparation of the library.</small></p>
</div>
<div class="small-6 columns"><center>Poplar</center><center><img src="https://www.diagenode.com/img/landing-pages/poplar.jpg" /></center>
<p><small><strong>Figure 3.</strong> ChIP-seq was performed on Populus trichocarpa stem differenciating xylem using the Premium H3K4me3 ChIP-seq grade antibody. Libraries were prepared with the <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Library Preparation&trade; kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in green represents the input and is considered as the background enrichment. The profile in red represents enrichments along a wide region of scaffold 18. Using the same scale, the peaks of the immunoprecipitated samples are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns"><center>Tomato</center><center><img src="https://www.diagenode.com/img/landing-pages/tomtato.jpg" /></center>
<p><small><strong>Figure 2.</strong> ChIP-seq was performed on Solanum lycopersicum cv. Micro-Tom young leaves using our Premium H3K4me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation&trade; kit</a> from 750 pg of immunoprecipitated DNA using the Universal Plant ChIP-seq kit (red) and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in blue represents a dataset obtained from Nguyen et al. 2014 that we used as an external reference. Enrichments are higher and consistent with the reference data along a wide region of chromosome 1.</small></p>
</div>
<div class="small-6 columns"><center>Maize</center><center><img src="https://www.diagenode.com/img/landing-pages/maize.jpg" /></center>
<p><small><strong>Figure 4.</strong> ChIP-seq was performed on Zea mays cv. B73 inner stem using our Premium H3K27me3 ChIP-seq grade antibody. Librairies were prepared with our <a href="https://www.diagenode.com/p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns">MicroPlex Librairy Preparation&trade; kit</a> from 1 ng of immunoprecipitated DNA using the Universal Plant ChIP-seq kit and 1 ng of Input and sequenced on an Illumina&reg; HiSeq 2500. The enrichment in green represents the Input and is considered as the background enrichment. The enrichment in red represents enrichments along a wide region of chromosome 3. Using the same scale, the peaks of the immunoprecipitated sample are significantly higher than those of the input, indicating a successful ChIP-seq experiment.</small></p>
</div>
</div>
<table style="width: 856px;">
<tbody>
<tr>
<td style="width: 224px;">
<h4><strong>Plant Species</strong></h4>
</td>
<td style="width: 341px;">
<h4><strong>Validated antibodies</strong></h4>
</td>
<td style="width: 357px;">
<h4><strong>Validated primer pairs</strong></h4>
</td>
</tr>
<tr>
<td style="width: 224px;"><strong>Arabidopsis (<em>Arabidopsis thaliana</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-actin-atg-primer-pair-50-ul">Arabidopsis Actin ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-monoclonal-antibody-classic-50-ug-50-ul">H3K4me3 monoclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-atg-primer-pair-50-ul">Arabidopsis FLC-ATG primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/arabidopsis-flc-intron1-primer-pair-50-ul">Arabidopsis FLC-intron1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me3-polyclonal-antibody-classic-sample-size-10-ug">H3K9me3 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9-14ac-polyclonal-antibody-classic-sample-size-10-mg">H3K9/14ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27ac-polyclonal-antibody-premium-sample-size-10-ug">H3K27ac polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Maize (<em>Zea mays</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/maize-B73-inner-stem-ZmB1-UTR-primer-pair-50ul">Maize B73 inner stem ZmB1-UTR primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/Maize-B73-inner-stem-ZmCopia-primer-pair-50ul">Maize B73 inner stem ZmCopia primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Tomato (<em>Solanum lycopersicum</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr2-reg8-primer-pair-50ul">Tomato leaves SlChr2-reg8 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/tomato-leaves-SlChr4-NC1-primer-pair-50ul">Tomato leaves SlChr4-NC1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Rice (<em>Oriza sativa</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsChr4-reg9-primer-pair-50ul">Rice seedlings OsChr4-reg9 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9me2-polyclonal-antibody-classic-50-ug-44-ul">H3K9me2 polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/rice-seedlings-OsMADS6-primer-pair-50ul">Rice seedlings OsMADS6 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k36me3-polyclonal-antibody-premium-sample-size-10-ug">H3K36me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"><strong>Poplar (<em>Populus trichocarpa, Populus tremula x alba</em>)</strong></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k4me3-polyclonal-antibody-premium-sample-size-10-ug">H3K4me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrCopia-orth-primer-pair-50ul">Poplar xylem PtrCopia-orth primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k9ac-polyclonal-antibody-classic-sample-size-10-ug">H3K9ac polyclonal antibody - Classic</a></td>
<td style="width: 357px;"><a href="https://www.diagenode.com/p/poplar-xylem-PtrMYBTF1-primer-pair-50ul">Poplar xylem PtrMYBTF1 primer pair</a></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3pan-monoclonal-antibody-classic-50-mg-100-ml">H3pan monoclonal antibody - Classic</a></td>
<td style="width: 357px;"></td>
</tr>
<tr>
<td style="width: 224px;"></td>
<td style="width: 341px;"><a href="https://www.diagenode.com/p/h3k27me3-polyclonal-antibody-premium-sample-size-10-ug">H3K27me3 polyclonal antibody - Premium</a></td>
<td style="width: 357px;"></td>
</tr>
</tbody>
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Universal Plant ChIP-seq kit

Auto Universal Plant ChIP-seq kit

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