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Rsf1 monoclonal antibody

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Antibodies

Catalog Number

Format

Price

C15200041
(MAb-041-050)

50 µg/39 µl

$380.00
Bulk order

Alternative names:HBXAP, RSF-1, XAP8, p325

Monoclonal antibody raised in mouse using a recombinant human RSF1 (remodeling and spacing factor 1) protein.

Lot	001
Concentration	1.3 µg/µl
Species reactivity	Human
Type	Monoclonal
Purity	Protein G purified
Host	Mouse
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
Western Blotting	1:500	Fig 1
Immunofluorescence	1:100 - 1:500	Ref 1
Immunoprecipitation	0.3 μg	Ref 1

Validation Data

Figure 1. Western blot analysis using the Diagenode monoclonal antibody against hRSF1
Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 20 μg) using the Diagenode monoclonal antibody directed against hRSF1 (cat# MAb-041-050), diluted 1:500 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left, the position of the protein of interest is shown on the right

実験手法

WB
Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more

IF
Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more

IP
Immunoprecipitation Read more

資料
Datasheet hRSF1 MAb-041-050 DATASHEET
Datasheet description Download

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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Cost-effective (requires less antibody per reaction)</li>
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			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
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<tr>
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<td>Ref 1</td>
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<p><small><strong> Figure 1. Western blot analysis using the Diagenode monoclonal antibody against hRSF1 </strong><br />Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 20 &mu;g) using the Diagenode monoclonal antibody directed against hRSF1 (cat# MAb-041-050), diluted 1:500 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left, the position of the protein of interest is shown on the right </small></p>
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<td>1:500</td>
<td>Fig 1</td>
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<td>1:100 - 1:500</td>
<td>Ref 1</td>
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<td>Ref 1</td>
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			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_title' => 'Chromatin Modifying Proteins - Antibodies | Diagenode',
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			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
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			'description' => '<p>Alternative names:<strong>HBXAP</strong>, <strong>RSF-1</strong>, <strong>XAP8</strong>, <strong>p325</strong></p>
<p><span>Monoclonal antibody raised in mouse using a recombinant human RSF1 (remodeling and spacing factor 1) protein.</span></p>',
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<div class="small-8 columns">
<p><small><strong> Figure 1. Western blot analysis using the Diagenode monoclonal antibody against hRSF1 </strong><br />Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 20 &mu;g) using the Diagenode monoclonal antibody directed against hRSF1 (cat# MAb-041-050), diluted 1:500 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left, the position of the protein of interest is shown on the right </small></p>
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			'name' => 'Rsf1 monoclonal antibody',
			'description' => 'RSF1 (UniProtKB/Swiss-Prot entry Q96T23) is required for assembly of regular nucleosome arrays by the RSF chromatin remodelling complex. RSF1 facilitates transcription of hepatitis B virus (HBV) genes by the pX transcription activator. In case of infection by HBV, together with pX, it represses TNF-alpha induced NF-kappaB transcription activation. RSF1 represses transcription when artificially recruited to chromatin by fusion to a heterogeneous DNA binding domain.
RSF1 interacts with SMARCA5/SNF2H to form the RSF complex and also binds the HBV pX/HBx protein, which is required to activate transcription of the viral genome.',
			'clonality' => '',
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			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
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<tbody>
<tr>
<td>Western Blotting</td>
<td>1:500</td>
<td>Fig 1</td>
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<tr>
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<td>Ref 1</td>
</tr>
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<p><small><strong> Figure 1. Western blot analysis using the Diagenode monoclonal antibody against hRSF1 </strong><br />Western blot was performed on nuclear extracts from HeLa cells (HeLa NE, 20 &mu;g) using the Diagenode monoclonal antibody directed against hRSF1 (cat# MAb-041-050), diluted 1:500 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left, the position of the protein of interest is shown on the right </small></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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	'id' => '3042',
	'name' => 'Rsf1 Antibody SDS BE nl',
	'language' => 'nl',
	'url' => 'files/SDS/Rsf1/SDS-C15200041-Rsf1_Antibody-BE-nl-GHS_1_0.pdf',
	'countries' => 'BE',
	'modified' => '2023-01-10 15:45:22',
	'created' => '2023-01-10 15:45:22',
	'ProductsSafetySheet' => array(
		'id' => '4994',
		'product_id' => '1976',
		'safety_sheet_id' => '3042'
	)
)
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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