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Polyclonal antibody raised in rabbit against drosophila Rtf1 (Rtf1, Paf1/RNA polymerase II complex component, homolog), using the full length recombinant protein.
Lot
001
Concentration
1.8 µg/µl
Species reactivity
Drosophila
Type
Polyclonal
Purity
Protein G purified
Host
Rabbit
Precautions
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Figure 1. Western blot analysis using the Diagenode antibody directed against dRtf1 Whole cell extracts (WCE) from drosophila larva and drosophila adults were analysed by Western blot using the Diagenode antibody against dRtf1 (Cat. No. pAb-018-050), diluted 1:1000. Size and location of the protein are indicated.
WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.
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IF Immunofluorescence:
Diagenode offers huge selection of highly sensitive antibodies validated in IF.
Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9
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ChIP-qPCR
定量のPCRとクロマチン免疫沈降（ChIP）が相まり、既知のゲノム結合部位でのタンパク質-DNA相互作用を調べる事に利用できます。ゲノム結合部位が不明の場合は、プロモーターのような潜在的な制御領域に対してqPCRプライマーを設計することもできます。ChIP-qPCRは、リアルタイムPCRを実行するコストが最小であるため、異なる実験条件にわたって特定の遺伝子および潜在的な制御領域に焦点を当てた研究においてとても有利です。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、およ... Read more
