Rtf1 polyclonal antibody

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			'description' => '<p><span>Alternative names: <strong>CDG1N</strong>, <strong>GTL7</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against drosophila Rtf1 (Rtf1, Paf1/RNA polymerase II complex component, homolog), using the full length recombinant protein.</span></p>',
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<p><small><strong> Figure 1. Western blot analysis using the Diagenode antibody directed against dRtf1 </strong><br />Whole cell extracts (WCE) from drosophila larva and drosophila adults were analysed by Western blot using the Diagenode antibody against dRtf1 (Cat. No. pAb-018-050), diluted 1:1000. Size and location of the protein are indicated. </small></p>
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<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 1, Ref 1</td>
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<td>Immunofluorescence</td>
<td>1:50</td>
<td>Ref 1</td>
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<td>Immunoprecipitation</td>
<td>12 &mu;g/IP</td>
<td>Ref 1</td>
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<td>1 - 5 &mu;g/ChIP</td>
<td>Ref 1</td>
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		'description' => '<p><span>Alternative names: <strong>CDG1N</strong>, <strong>GTL7</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against drosophila Rtf1 (Rtf1, Paf1/RNA polymerase II complex component, homolog), using the full length recombinant protein.</span></p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410018_fig1.png" alt="WB" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 1. Western blot analysis using the Diagenode antibody directed against dRtf1 </strong><br />Whole cell extracts (WCE) from drosophila larva and drosophila adults were analysed by Western blot using the Diagenode antibody against dRtf1 (Cat. No. pAb-018-050), diluted 1:1000. Size and location of the protein are indicated. </small></p>
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<td>1:1,000</td>
<td>Fig 1, Ref 1</td>
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<td>1:50</td>
<td>Ref 1</td>
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<td>12 &mu;g/IP</td>
<td>Ref 1</td>
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<td>Ref 1</td>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong>&nbsp;against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
<li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li>
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<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
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			'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>',
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<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
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<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
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<li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong>&nbsp;against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
<li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li>
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<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
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<div class="row">定量のPCRとクロマチン免疫沈降（ChIP）が相まり、既知のゲノム結合部位でのタンパク質-DNA相互作用を調べる事に利用できます。ゲノム結合部位が不明の場合は、プロモーターのような潜在的な制御領域に対してqPCRプライマーを設計することもできます。ChIP-qPCRは、リアルタイムPCRを実行するコストが最小であるため、異なる実験条件にわたって特定の遺伝子および潜在的な制御領域に焦点を当てた研究においてとても有利です。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、および遺伝子発現に対するヒストン修飾の効果を含む様々なライフサイエンス分野で使用されています。<br />
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
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<li class="large-12 columns"><strong>Chromatin preparation (クロマチン調製):<span>&nbsp;</span></strong>DNAへのヒストンまたは転写因子などのクロマチン結合タンパク質の固定（架橋）に続いて細胞溶解。</li>
<li class="large-12 columns"><strong><strong><strong>Chromatin shearing (クロマチン断片化):<span>&nbsp;</span></strong></strong></strong>超音波処理による所望の断片サイズ（100〜500bp）までのクロマチンの断片化</li>
<li class="large-12 columns"><strong>Chromatin IP (クロマチン免疫沈降):</strong><span>&nbsp;</span>目的のヒストンまたは転写因子に対する<strong><strong><a href="./chip-qpcr-antibodies">特定のChIP級抗体</a></strong></strong>
<p>を用いたタンパク質-DNA複合体の捕捉</p>
</li>
<li class="large-12 columns"><strong>DNA purification (DNA精製):<span>&nbsp;</span></strong>クロマチン逆架橋および溶出後の精製</li>
<li class="large-12 columns"><strong>qPCR and analysis (qPCRおよび分析):</strong><span>&nbsp;</span>以前に設計されたプライマーを使用して、特定の遺伝子座位で免疫沈降した物質を増幅する</li>
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<h3 class="text-center" style="color: #b21329;">初めての方へ</h3>
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<div class="row">定量のPCRとクロマチン免疫沈降（ChIP）が相まり、既知のゲノム結合部位でのタンパク質-DNA相互作用を調べる事に利用できます。ゲノム結合部位が不明の場合は、プロモーターのような潜在的な制御領域に対してqPCRプライマーを設計することもできます。ChIP-qPCRは、リアルタイムPCRを実行するコストが最小であるため、異なる実験条件にわたって特定の遺伝子および潜在的な制御領域に焦点を当てた研究においてとても有利です。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、および遺伝子発現に対するヒストン修飾の効果を含む様々なライフサイエンス分野で使用されています。<br />
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
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<li class="large-12 columns"><strong>Chromatin preparation (クロマチン調製):<span>&nbsp;</span></strong>DNAへのヒストンまたは転写因子などのクロマチン結合タンパク質の固定（架橋）に続いて細胞溶解。</li>
<li class="large-12 columns"><strong><strong><strong>Chromatin shearing (クロマチン断片化):<span>&nbsp;</span></strong></strong></strong>超音波処理による所望の断片サイズ（100〜500bp）までのクロマチンの断片化</li>
<li class="large-12 columns"><strong>Chromatin IP (クロマチン免疫沈降):</strong><span>&nbsp;</span>目的のヒストンまたは転写因子に対する<strong><strong><a href="./chip-qpcr-antibodies">特定のChIP級抗体</a></strong></strong>
<p>を用いたタンパク質-DNA複合体の捕捉</p>
</li>
<li class="large-12 columns"><strong>DNA purification (DNA精製):<span>&nbsp;</span></strong>クロマチン逆架橋および溶出後の精製</li>
<li class="large-12 columns"><strong>qPCR and analysis (qPCRおよび分析):</strong><span>&nbsp;</span>以前に設計されたプライマーを使用して、特定の遺伝子座位で免疫沈降した物質を増幅する</li>
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<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">初めての方へ</h3>
<p>当社の完全なChIPキットを選択頂くか、個別で抗体、バッファー、ビーズ、クロマチン断片および精製試薬から必要なものを選択頂けます。ChIP Kit Customizerを使用すると、検証済みのChIPキットから必要なアイテムを自由に選択できます。</p>
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<div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]
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<p><span>Polyclonal antibody raised in rabbit against drosophila Rtf1 (Rtf1, Paf1/RNA polymerase II complex component, homolog), using the full length recombinant protein.</span></p>',
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<p><small><strong> Figure 1. Western blot analysis using the Diagenode antibody directed against dRtf1 </strong><br />Whole cell extracts (WCE) from drosophila larva and drosophila adults were analysed by Western blot using the Diagenode antibody against dRtf1 (Cat. No. pAb-018-050), diluted 1:1000. Size and location of the protein are indicated. </small></p>
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<p><small><strong> Figure 1. Western blot analysis using the Diagenode antibody directed against dRtf1 </strong><br />Whole cell extracts (WCE) from drosophila larva and drosophila adults were analysed by Western blot using the Diagenode antibody against dRtf1 (Cat. No. pAb-018-050), diluted 1:1000. Size and location of the protein are indicated. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
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<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
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<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong>&nbsp;against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
<li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li>
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<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
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<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong>&nbsp;against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
<li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li>
</ol>
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<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
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	'meta_description' => 'Diagenode's ChIP qPCR kits can be used to quantify enriched DNA after chromatin immunoprecipitation. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of',
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<div class="row">定量のPCRとクロマチン免疫沈降（ChIP）が相まり、既知のゲノム結合部位でのタンパク質-DNA相互作用を調べる事に利用できます。ゲノム結合部位が不明の場合は、プロモーターのような潜在的な制御領域に対してqPCRプライマーを設計することもできます。ChIP-qPCRは、リアルタイムPCRを実行するコストが最小であるため、異なる実験条件にわたって特定の遺伝子および潜在的な制御領域に焦点を当てた研究においてとても有利です。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、および遺伝子発現に対するヒストン修飾の効果を含む様々なライフサイエンス分野で使用されています。<br />
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation (クロマチン調製):<span>&nbsp;</span></strong>DNAへのヒストンまたは転写因子などのクロマチン結合タンパク質の固定（架橋）に続いて細胞溶解。</li>
<li class="large-12 columns"><strong><strong><strong>Chromatin shearing (クロマチン断片化):<span>&nbsp;</span></strong></strong></strong>超音波処理による所望の断片サイズ（100〜500bp）までのクロマチンの断片化</li>
<li class="large-12 columns"><strong>Chromatin IP (クロマチン免疫沈降):</strong><span>&nbsp;</span>目的のヒストンまたは転写因子に対する<strong><strong><a href="./chip-qpcr-antibodies">特定のChIP級抗体</a></strong></strong>
<p>を用いたタンパク質-DNA複合体の捕捉</p>
</li>
<li class="large-12 columns"><strong>DNA purification (DNA精製):<span>&nbsp;</span></strong>クロマチン逆架橋および溶出後の精製</li>
<li class="large-12 columns"><strong>qPCR and analysis (qPCRおよび分析):</strong><span>&nbsp;</span>以前に設計されたプライマーを使用して、特定の遺伝子座位で免疫沈降した物質を増幅する</li>
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<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">初めての方へ</h3>
<p>当社の完全なChIPキットを選択頂くか、個別で抗体、バッファー、ビーズ、クロマチン断片および精製試薬から必要なものを選択頂けます。ChIP Kit Customizerを使用すると、検証済みのChIPキットから必要なアイテムを自由に選択できます。</p>
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<div class="row">定量のPCRとクロマチン免疫沈降（ChIP）が相まり、既知のゲノム結合部位でのタンパク質-DNA相互作用を調べる事に利用できます。ゲノム結合部位が不明の場合は、プロモーターのような潜在的な制御領域に対してqPCRプライマーを設計することもできます。ChIP-qPCRは、リアルタイムPCRを実行するコストが最小であるため、異なる実験条件にわたって特定の遺伝子および潜在的な制御領域に焦点を当てた研究においてとても有利です。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、および遺伝子発現に対するヒストン修飾の効果を含む様々なライフサイエンス分野で使用されています。<br />
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation (クロマチン調製):<span>&nbsp;</span></strong>DNAへのヒストンまたは転写因子などのクロマチン結合タンパク質の固定（架橋）に続いて細胞溶解。</li>
<li class="large-12 columns"><strong><strong><strong>Chromatin shearing (クロマチン断片化):<span>&nbsp;</span></strong></strong></strong>超音波処理による所望の断片サイズ（100〜500bp）までのクロマチンの断片化</li>
<li class="large-12 columns"><strong>Chromatin IP (クロマチン免疫沈降):</strong><span>&nbsp;</span>目的のヒストンまたは転写因子に対する<strong><strong><a href="./chip-qpcr-antibodies">特定のChIP級抗体</a></strong></strong>
<p>を用いたタンパク質-DNA複合体の捕捉</p>
</li>
<li class="large-12 columns"><strong>DNA purification (DNA精製):<span>&nbsp;</span></strong>クロマチン逆架橋および溶出後の精製</li>
<li class="large-12 columns"><strong>qPCR and analysis (qPCRおよび分析):</strong><span>&nbsp;</span>以前に設計されたプライマーを使用して、特定の遺伝子座位で免疫沈降した物質を増幅する</li>
</ol>
</div>
</div>
<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">初めての方へ</h3>
<p>当社の完全なChIPキットを選択頂くか、個別で抗体、バッファー、ビーズ、クロマチン断片および精製試薬から必要なものを選択頂けます。ChIP Kit Customizerを使用すると、検証済みのChIPキットから必要なアイテムを自由に選択できます。</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
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Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]
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			'description' => '<p><span>Alternative names: <strong>CDG1N</strong>, <strong>GTL7</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against drosophila Rtf1 (Rtf1, Paf1/RNA polymerase II complex component, homolog), using the full length recombinant protein.</span></p>',
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<p><small><strong> Figure 1. Western blot analysis using the Diagenode antibody directed against dRtf1 </strong><br />Whole cell extracts (WCE) from drosophila larva and drosophila adults were analysed by Western blot using the Diagenode antibody against dRtf1 (Cat. No. pAb-018-050), diluted 1:1000. Size and location of the protein are indicated. </small></p>
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			'name' => 'Rtf1 polyclonal antibody',
			'description' => 'PAF is a five-subunit protein complex composed of Paf1, Cdc73, Leo1, Rtf1 and Ctr9, which was first purified from yeast. The yeast PAF (yPAF) complex interacts with RNA polymerase II and coordinates the setting of histone marks associated with active transcription. The human PAF (hPAF) complex shares four subunits with yPAF (hCtr9, hPaf1, hLeo1 and Cdc73), but contains an additional higher eukaryotic-specific subunit, hSki8. In addition to coordinating events during transcription (initiation, promoter clearance and elongation); PAF also coordinates events in RNA quality control.',
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<p><small><strong> Figure 1. Western blot analysis using the Diagenode antibody directed against dRtf1 </strong><br />Whole cell extracts (WCE) from drosophila larva and drosophila adults were analysed by Western blot using the Diagenode antibody against dRtf1 (Cat. No. pAb-018-050), diluted 1:1000. Size and location of the protein are indicated. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
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<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
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<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong>&nbsp;against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
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<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
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<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
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<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
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<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
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<li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong>&nbsp;against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
<li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li>
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<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
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	'meta_description' => 'Diagenode's ChIP qPCR kits can be used to quantify enriched DNA after chromatin immunoprecipitation. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of',
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<div class="row">定量のPCRとクロマチン免疫沈降（ChIP）が相まり、既知のゲノム結合部位でのタンパク質-DNA相互作用を調べる事に利用できます。ゲノム結合部位が不明の場合は、プロモーターのような潜在的な制御領域に対してqPCRプライマーを設計することもできます。ChIP-qPCRは、リアルタイムPCRを実行するコストが最小であるため、異なる実験条件にわたって特定の遺伝子および潜在的な制御領域に焦点を当てた研究においてとても有利です。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、および遺伝子発現に対するヒストン修飾の効果を含む様々なライフサイエンス分野で使用されています。<br />
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation (クロマチン調製):<span>&nbsp;</span></strong>DNAへのヒストンまたは転写因子などのクロマチン結合タンパク質の固定（架橋）に続いて細胞溶解。</li>
<li class="large-12 columns"><strong><strong><strong>Chromatin shearing (クロマチン断片化):<span>&nbsp;</span></strong></strong></strong>超音波処理による所望の断片サイズ（100〜500bp）までのクロマチンの断片化</li>
<li class="large-12 columns"><strong>Chromatin IP (クロマチン免疫沈降):</strong><span>&nbsp;</span>目的のヒストンまたは転写因子に対する<strong><strong><a href="./chip-qpcr-antibodies">特定のChIP級抗体</a></strong></strong>
<p>を用いたタンパク質-DNA複合体の捕捉</p>
</li>
<li class="large-12 columns"><strong>DNA purification (DNA精製):<span>&nbsp;</span></strong>クロマチン逆架橋および溶出後の精製</li>
<li class="large-12 columns"><strong>qPCR and analysis (qPCRおよび分析):</strong><span>&nbsp;</span>以前に設計されたプライマーを使用して、特定の遺伝子座位で免疫沈降した物質を増幅する</li>
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<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">初めての方へ</h3>
<p>当社の完全なChIPキットを選択頂くか、個別で抗体、バッファー、ビーズ、クロマチン断片および精製試薬から必要なものを選択頂けます。ChIP Kit Customizerを使用すると、検証済みのChIPキットから必要なアイテムを自由に選択できます。</p>
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<div class="row">定量のPCRとクロマチン免疫沈降（ChIP）が相まり、既知のゲノム結合部位でのタンパク質-DNA相互作用を調べる事に利用できます。ゲノム結合部位が不明の場合は、プロモーターのような潜在的な制御領域に対してqPCRプライマーを設計することもできます。ChIP-qPCRは、リアルタイムPCRを実行するコストが最小であるため、異なる実験条件にわたって特定の遺伝子および潜在的な制御領域に焦点を当てた研究においてとても有利です。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、および遺伝子発現に対するヒストン修飾の効果を含む様々なライフサイエンス分野で使用されています。<br />
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation (クロマチン調製):<span>&nbsp;</span></strong>DNAへのヒストンまたは転写因子などのクロマチン結合タンパク質の固定（架橋）に続いて細胞溶解。</li>
<li class="large-12 columns"><strong><strong><strong>Chromatin shearing (クロマチン断片化):<span>&nbsp;</span></strong></strong></strong>超音波処理による所望の断片サイズ（100〜500bp）までのクロマチンの断片化</li>
<li class="large-12 columns"><strong>Chromatin IP (クロマチン免疫沈降):</strong><span>&nbsp;</span>目的のヒストンまたは転写因子に対する<strong><strong><a href="./chip-qpcr-antibodies">特定のChIP級抗体</a></strong></strong>
<p>を用いたタンパク質-DNA複合体の捕捉</p>
</li>
<li class="large-12 columns"><strong>DNA purification (DNA精製):<span>&nbsp;</span></strong>クロマチン逆架橋および溶出後の精製</li>
<li class="large-12 columns"><strong>qPCR and analysis (qPCRおよび分析):</strong><span>&nbsp;</span>以前に設計されたプライマーを使用して、特定の遺伝子座位で免疫沈降した物質を増幅する</li>
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</div>
<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">初めての方へ</h3>
<p>当社の完全なChIPキットを選択頂くか、個別で抗体、バッファー、ビーズ、クロマチン断片および精製試薬から必要なものを選択頂けます。ChIP Kit Customizerを使用すると、検証済みのChIPキットから必要なアイテムを自由に選択できます。</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
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Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]
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			'description' => '<p><span>Alternative names: <strong>CDG1N</strong>, <strong>GTL7</strong></span></p>
<p><span>Polyclonal antibody raised in rabbit against drosophila Rtf1 (Rtf1, Paf1/RNA polymerase II complex component, homolog), using the full length recombinant protein.</span></p>',
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<p><small><strong> Figure 1. Western blot analysis using the Diagenode antibody directed against dRtf1 </strong><br />Whole cell extracts (WCE) from drosophila larva and drosophila adults were analysed by Western blot using the Diagenode antibody against dRtf1 (Cat. No. pAb-018-050), diluted 1:1000. Size and location of the protein are indicated. </small></p>
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			'id' => '279',
			'name' => 'Rtf1 polyclonal antibody',
			'description' => 'PAF is a five-subunit protein complex composed of Paf1, Cdc73, Leo1, Rtf1 and Ctr9, which was first purified from yeast. The yeast PAF (yPAF) complex interacts with RNA polymerase II and coordinates the setting of histone marks associated with active transcription. The human PAF (hPAF) complex shares four subunits with yPAF (hCtr9, hPaf1, hLeo1 and Cdc73), but contains an additional higher eukaryotic-specific subunit, hSki8. In addition to coordinating events during transcription (initiation, promoter clearance and elongation); PAF also coordinates events in RNA quality control.',
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			'isotype' => '',
			'lot' => '001',
			'concentration' => '1.8 &micro;g/&micro;l',
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			'classification' => '',
			'application_table' => '<table>
<thead>
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<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
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<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 1, Ref 1</td>
</tr>
<tr>
<td>Immunofluorescence</td>
<td>1:50</td>
<td>Ref 1</td>
</tr>
<tr>
<td>Immunoprecipitation</td>
<td>12 &mu;g/IP</td>
<td>Ref 1</td>
</tr>
<tr>
<td>ChIP</td>
<td>1 - 5 &mu;g/ChIP</td>
<td>Ref 1</td>
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<p><small><strong> Figure 1. Western blot analysis using the Diagenode antibody directed against dRtf1 </strong><br />Whole cell extracts (WCE) from drosophila larva and drosophila adults were analysed by Western blot using the Diagenode antibody against dRtf1 (Cat. No. pAb-018-050), diluted 1:1000. Size and location of the protein are indicated. </small></p>
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<td>Western Blotting</td>
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<td>Fig 1, Ref 1</td>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
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<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
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<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong>&nbsp;against the histone or transcription factor of interest</li>
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<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
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<div class="small-12 medium-12 large-12 columns text-justify">
<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong>&nbsp;against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
<li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li>
</ol>
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<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
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	'meta_keywords' => 'Chromatin immunoprecipitation,ChIP Quantitative PCR,polymerase chain reaction (PCR)',
	'meta_description' => 'Diagenode's ChIP qPCR kits can be used to quantify enriched DNA after chromatin immunoprecipitation. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of',
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	'description' => '<div class="row">
<div class="row">定量のPCRとクロマチン免疫沈降（ChIP）が相まり、既知のゲノム結合部位でのタンパク質-DNA相互作用を調べる事に利用できます。ゲノム結合部位が不明の場合は、プロモーターのような潜在的な制御領域に対してqPCRプライマーを設計することもできます。ChIP-qPCRは、リアルタイムPCRを実行するコストが最小であるため、異なる実験条件にわたって特定の遺伝子および潜在的な制御領域に焦点を当てた研究においてとても有利です。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、および遺伝子発現に対するヒストン修飾の効果を含む様々なライフサイエンス分野で使用されています。<br />
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation (クロマチン調製):<span>&nbsp;</span></strong>DNAへのヒストンまたは転写因子などのクロマチン結合タンパク質の固定（架橋）に続いて細胞溶解。</li>
<li class="large-12 columns"><strong><strong><strong>Chromatin shearing (クロマチン断片化):<span>&nbsp;</span></strong></strong></strong>超音波処理による所望の断片サイズ（100〜500bp）までのクロマチンの断片化</li>
<li class="large-12 columns"><strong>Chromatin IP (クロマチン免疫沈降):</strong><span>&nbsp;</span>目的のヒストンまたは転写因子に対する<strong><strong><a href="./chip-qpcr-antibodies">特定のChIP級抗体</a></strong></strong>
<p>を用いたタンパク質-DNA複合体の捕捉</p>
</li>
<li class="large-12 columns"><strong>DNA purification (DNA精製):<span>&nbsp;</span></strong>クロマチン逆架橋および溶出後の精製</li>
<li class="large-12 columns"><strong>qPCR and analysis (qPCRおよび分析):</strong><span>&nbsp;</span>以前に設計されたプライマーを使用して、特定の遺伝子座位で免疫沈降した物質を増幅する</li>
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<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">初めての方へ</h3>
<p>当社の完全なChIPキットを選択頂くか、個別で抗体、バッファー、ビーズ、クロマチン断片および精製試薬から必要なものを選択頂けます。ChIP Kit Customizerを使用すると、検証済みのChIPキットから必要なアイテムを自由に選択できます。</p>
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<div class="row">定量のPCRとクロマチン免疫沈降（ChIP）が相まり、既知のゲノム結合部位でのタンパク質-DNA相互作用を調べる事に利用できます。ゲノム結合部位が不明の場合は、プロモーターのような潜在的な制御領域に対してqPCRプライマーを設計することもできます。ChIP-qPCRは、リアルタイムPCRを実行するコストが最小であるため、異なる実験条件にわたって特定の遺伝子および潜在的な制御領域に焦点を当てた研究においてとても有利です。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、および遺伝子発現に対するヒストン修飾の効果を含む様々なライフサイエンス分野で使用されています。<br />
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation (クロマチン調製):<span>&nbsp;</span></strong>DNAへのヒストンまたは転写因子などのクロマチン結合タンパク質の固定（架橋）に続いて細胞溶解。</li>
<li class="large-12 columns"><strong><strong><strong>Chromatin shearing (クロマチン断片化):<span>&nbsp;</span></strong></strong></strong>超音波処理による所望の断片サイズ（100〜500bp）までのクロマチンの断片化</li>
<li class="large-12 columns"><strong>Chromatin IP (クロマチン免疫沈降):</strong><span>&nbsp;</span>目的のヒストンまたは転写因子に対する<strong><strong><a href="./chip-qpcr-antibodies">特定のChIP級抗体</a></strong></strong>
<p>を用いたタンパク質-DNA複合体の捕捉</p>
</li>
<li class="large-12 columns"><strong>DNA purification (DNA精製):<span>&nbsp;</span></strong>クロマチン逆架橋および溶出後の精製</li>
<li class="large-12 columns"><strong>qPCR and analysis (qPCRおよび分析):</strong><span>&nbsp;</span>以前に設計されたプライマーを使用して、特定の遺伝子座位で免疫沈降した物質を増幅する</li>
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</div>
<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">初めての方へ</h3>
<p>当社の完全なChIPキットを選択頂くか、個別で抗体、バッファー、ビーズ、クロマチン断片および精製試薬から必要なものを選択頂けます。ChIP Kit Customizerを使用すると、検証済みのChIPキットから必要なアイテムを自由に選択できます。</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118