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SAP30 Antibody - ChIP-seq Grade (sample size)

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

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Antibodies

Catalog Number

Format

Price

C15410036-10

10 µg

$115.00
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Other format

C15410036

50 µg

Polyclonal antibody raised in rabbit against human SAP30 (Sin3-associated polypeptide, 30kDa), using the full length His-tagged protein.

Lot	001
Concentration	2.0 µg/µl
Species reactivity	Human
Type	Polyclonal
Purity	Protein G purified
Host	Rabbit
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ChIP/ChIP-seq ^*	2-5 μg/ChIP	Fig 1, 2
Western Blotting	1:1;000	Fig 3

^* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

Validation Data

Figure 1. ChIP results obtained with the Diagenode antibody directed against SAP30
ChIP assays were performed using HeLa cells, the Diagenode antibody against SAP30 (Cat. No. C15410036) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/ IP) was used as negative IP control. QPCR was performed with primers for the EIF2S3 and BRCA1 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SAP30
ChIP was performed on sheared chromatin from 4 million HeLa cells as described above using 5 μg of the Diagenode antibody against SAP30 (Cat. No. C15410036). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of human chromosome 1 (fig 2A and B) and in two genomic regions surrounding the BRCA1 and EIF2S3 genes on chromosome 17 and X, respectively (fig 2C and D).

Figure 3. Western blot analysis using the Diagenode antibody directed against SAP30
Western blot was performed on nuclear extracts from HeLa cells (20 μg) using the Diagenode antibody against SAP30 (Cat. No. C15410036) diluted 1:1000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.
Target Description
SAP30 (UniProtKB/Swiss-Prot entry O75446) is a component of the histone deacetylase complex, which also includes SIN3, SAP18, HDAC1, HDAC2, RbAp46, RbAp48. Histone acetylation and deacetylation play a key role in the regulation of eukaryotic gene expression. This complex only deacetylates core histone octamers, and is not able to deacetylate nucleosomal histones

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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SAP30</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SAP30 (Cat. No. C15410036) and optimized primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 &mu;g per ChIP experiment was analysed. IgG (1 &mu;g/ IP) was used as negative IP control. QPCR was performed with primers for the EIF2S3 and BRCA1 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SAP30</strong><br />ChIP was performed on sheared chromatin from 4 million HeLa cells as described above using 5 &mu;g of the Diagenode antibody against SAP30 (Cat. No. C15410036). The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of human chromosome 1 (fig 2A and B) and in two genomic regions surrounding the BRCA1 and EIF2S3 genes on chromosome 17 and X, respectively (fig 2C and D).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SAP30</strong><br />Western blot was performed on nuclear extracts from HeLa cells (20 &mu;g) using the Diagenode antibody against SAP30 (Cat. No. C15410036) diluted 1:1000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.</small></p>
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<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410036-chipseq-C.jpg" alt="SAP30 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SAP30</strong><br />ChIP was performed on sheared chromatin from 4 million HeLa cells as described above using 5 &mu;g of the Diagenode antibody against SAP30 (Cat. No. C15410036). The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of human chromosome 1 (fig 2A and B) and in two genomic regions surrounding the BRCA1 and EIF2S3 genes on chromosome 17 and X, respectively (fig 2C and D).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SAP30</strong><br />Western blot was performed on nuclear extracts from HeLa cells (20 &mu;g) using the Diagenode antibody against SAP30 (Cat. No. C15410036) diluted 1:1000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.</small></p>
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			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
</div>
<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
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<p></p>
<p></p>
<p></p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong>&nbsp;with rigorous QC and validation</li>
<li><strong>Classified</strong>&nbsp;based on level of validation for flexibility of application</li>
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<p>Existing sample sizes are listed below. We will soon expand our collection. &nbsp;Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
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		'description' => '<p><span>Polyclonal antibody raised in rabbit against human<strong> SAP30 (Sin3-associated polypeptide, 30kDa)</strong>, using the full length His-tagged protein.</span></p>',
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<div class="small-4 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410036-chip.jpg" alt="SAP30 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SAP30</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SAP30 (Cat. No. C15410036) and optimized primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 &mu;g per ChIP experiment was analysed. IgG (1 &mu;g/ IP) was used as negative IP control. QPCR was performed with primers for the EIF2S3 and BRCA1 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410036-chipseq-A.jpg" alt="SAP30 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410036-chipseq-B.jpg" alt="SAP30 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410036-chipseq-C.jpg" alt="SAP30 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410036-chipseq-D.jpg" alt="SAP30 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SAP30</strong><br />ChIP was performed on sheared chromatin from 4 million HeLa cells as described above using 5 &mu;g of the Diagenode antibody against SAP30 (Cat. No. C15410036). The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of human chromosome 1 (fig 2A and B) and in two genomic regions surrounding the BRCA1 and EIF2S3 genes on chromosome 17 and X, respectively (fig 2C and D).</small></p>
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<div class="small-4 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410036-wb.jpg" alt="SAP30 Antibody validated in Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SAP30</strong><br />Western blot was performed on nuclear extracts from HeLa cells (20 &mu;g) using the Diagenode antibody against SAP30 (Cat. No. C15410036) diluted 1:1000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.</small></p>
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View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
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Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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		'concentration' => '2.0 &micro;g/&micro;l',
		'reactivity' => 'Human',
		'type' => 'Polyclonal',
		'purity' => 'Protein G purified',
		'classification' => '',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2-5 &mu;g/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1;000</td>
<td>Fig 3</td>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 &mu;g per IP.</small></p>',
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		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
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		'slug' => 'sap30-polyclonal-antibody-pioneer-10',
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		'modified' => '2020-11-25 16:18:01',
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			'antibody_id' => '227',
			'name' => 'SAP30 Antibody',
			'description' => '<p><span>Polyclonal antibody raised in rabbit against human<strong> SAP30 (Sin3-associated polypeptide, 30kDa)</strong>, using the full length His-tagged protein.</span></p>',
			'label1' => 'Validation Data',
			'info1' => '<div class="row">
<div class="small-4 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410036-chip.jpg" alt="SAP30 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-8 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SAP30</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SAP30 (Cat. No. C15410036) and optimized primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 &mu;g per ChIP experiment was analysed. IgG (1 &mu;g/ IP) was used as negative IP control. QPCR was performed with primers for the EIF2S3 and BRCA1 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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</div>
<div class="row">
<div class="small-6 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410036-chipseq-A.jpg" alt="SAP30 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410036-chipseq-B.jpg" alt="SAP30 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410036-chipseq-C.jpg" alt="SAP30 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410036-chipseq-D.jpg" alt="SAP30 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-6 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SAP30</strong><br />ChIP was performed on sheared chromatin from 4 million HeLa cells as described above using 5 &mu;g of the Diagenode antibody against SAP30 (Cat. No. C15410036). The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of human chromosome 1 (fig 2A and B) and in two genomic regions surrounding the BRCA1 and EIF2S3 genes on chromosome 17 and X, respectively (fig 2C and D).</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410036-wb.jpg" alt="SAP30 Antibody validated in Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SAP30</strong><br />Western blot was performed on nuclear extracts from HeLa cells (20 &mu;g) using the Diagenode antibody against SAP30 (Cat. No. C15410036) diluted 1:1000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.</small></p>
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			'meta_description' => 'SAP30 (Sin3-associated polypeptide) Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR and WB. Batch-specific data available on the website. ',
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			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
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			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
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			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
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			'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
</div>
<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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<div class="small-10 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
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<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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			'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP',
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			'description' => '<h1><strong>Validated epigenetics antibodies</strong>&nbsp;&ndash; care for a sample?<br />&nbsp;</h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong>&nbsp;with rigorous QC and validation</li>
<li><strong>Classified</strong>&nbsp;based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. &nbsp;Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SAP30</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SAP30 (Cat. No. C15410036) and optimized primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 &mu;g per ChIP experiment was analysed. IgG (1 &mu;g/ IP) was used as negative IP control. QPCR was performed with primers for the EIF2S3 and BRCA1 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SAP30</strong><br />ChIP was performed on sheared chromatin from 4 million HeLa cells as described above using 5 &mu;g of the Diagenode antibody against SAP30 (Cat. No. C15410036). The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of human chromosome 1 (fig 2A and B) and in two genomic regions surrounding the BRCA1 and EIF2S3 genes on chromosome 17 and X, respectively (fig 2C and D).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SAP30</strong><br />Western blot was performed on nuclear extracts from HeLa cells (20 &mu;g) using the Diagenode antibody against SAP30 (Cat. No. C15410036) diluted 1:1000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.</small></p>
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<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410036-chipseq-A.jpg" alt="SAP30 Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410036-chipseq-B.jpg" alt="SAP30 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410036-chipseq-C.jpg" alt="SAP30 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410036-chipseq-D.jpg" alt="SAP30 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SAP30</strong><br />ChIP was performed on sheared chromatin from 4 million HeLa cells as described above using 5 &mu;g of the Diagenode antibody against SAP30 (Cat. No. C15410036). The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of human chromosome 1 (fig 2A and B) and in two genomic regions surrounding the BRCA1 and EIF2S3 genes on chromosome 17 and X, respectively (fig 2C and D).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SAP30</strong><br />Western blot was performed on nuclear extracts from HeLa cells (20 &mu;g) using the Diagenode antibody against SAP30 (Cat. No. C15410036) diluted 1:1000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
</div>
<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SAP30</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SAP30 (Cat. No. C15410036) and optimized primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 &mu;g per ChIP experiment was analysed. IgG (1 &mu;g/ IP) was used as negative IP control. QPCR was performed with primers for the EIF2S3 and BRCA1 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SAP30</strong><br />ChIP was performed on sheared chromatin from 4 million HeLa cells as described above using 5 &mu;g of the Diagenode antibody against SAP30 (Cat. No. C15410036). The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of human chromosome 1 (fig 2A and B) and in two genomic regions surrounding the BRCA1 and EIF2S3 genes on chromosome 17 and X, respectively (fig 2C and D).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SAP30</strong><br />Western blot was performed on nuclear extracts from HeLa cells (20 &mu;g) using the Diagenode antibody against SAP30 (Cat. No. C15410036) diluted 1:1000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.</small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SAP30</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SAP30 (Cat. No. C15410036) and optimized primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 &mu;g per ChIP experiment was analysed. IgG (1 &mu;g/ IP) was used as negative IP control. QPCR was performed with primers for the EIF2S3 and BRCA1 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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View::render() - CORE/Cake/View/View.php, line 473
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ReflectionMethod::invokeArgs() - [internal], line ??
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SAP30</strong><br />ChIP was performed on sheared chromatin from 4 million HeLa cells as described above using 5 &mu;g of the Diagenode antibody against SAP30 (Cat. No. C15410036). The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of human chromosome 1 (fig 2A and B) and in two genomic regions surrounding the BRCA1 and EIF2S3 genes on chromosome 17 and X, respectively (fig 2C and D).</small></p>
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<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410036-wb.jpg" alt="SAP30 Antibody validated in Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SAP30</strong><br />Western blot was performed on nuclear extracts from HeLa cells (20 &mu;g) using the Diagenode antibody against SAP30 (Cat. No. C15410036) diluted 1:1000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode&rsquo;s highly validated antibodies:</p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SAP30</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SAP30 (Cat. No. C15410036) and optimized primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 &mu;g per ChIP experiment was analysed. IgG (1 &mu;g/ IP) was used as negative IP control. QPCR was performed with primers for the EIF2S3 and BRCA1 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410036-chipseq-C.jpg" alt="SAP30 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410036-chipseq-D.jpg" alt="SAP30 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SAP30</strong><br />ChIP was performed on sheared chromatin from 4 million HeLa cells as described above using 5 &mu;g of the Diagenode antibody against SAP30 (Cat. No. C15410036). The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of human chromosome 1 (fig 2A and B) and in two genomic regions surrounding the BRCA1 and EIF2S3 genes on chromosome 17 and X, respectively (fig 2C and D).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against SAP30</strong><br />Western blot was performed on nuclear extracts from HeLa cells (20 &mu;g) using the Diagenode antibody against SAP30 (Cat. No. C15410036) diluted 1:1000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.</small></p>
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<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410036-chipseq-B.jpg" alt="SAP30 Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410036-chipseq-C.jpg" alt="SAP30 Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p class="text-center"><img src="https://www.diagenode.com/img/product/antibodies/C15410036-chipseq-D.jpg" alt="SAP30 Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download
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SAP30 Antibody - ChIP-seq Grade (sample size)

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