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<p><small><strong> Figure 1. Western blot analysis using the Diagenode antibody directed against SKI3 </strong><br />Western blot was performed on HeLa nuclear extracts (lane 1) and on the purified human SKI complex (lane 2) (Figure 1A), or on nuclear extracts from HeLa cells (HeLa NE, 20 µg) (Figure 1B) using the Diagenode antibody against SKI3 (Cat. No. C15410011) diluted 1:100. The location of the protein is indicated on the right, the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong> Figure 2. Immunofluorescence with the Diagenode antibody directed against SKI3 </strong><br />HeLa cells were stained with the Diagenode antibody against SKI3 (Cat. No. C15410011) (in red), with DAPI to label the DNA (in blue), and with an antibody against α-tubulin to label the cytoplasm (in green). SKI3 is detected in both the cytoplasm and the nucleus but appears to be excluded from the nucleolus. </small></p>
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<p><small><strong> Figure 2. Immunofluorescence with the Diagenode antibody directed against SKI3 </strong><br />HeLa cells were stained with the Diagenode antibody against SKI3 (Cat. No. C15410011) (in red), with DAPI to label the DNA (in blue), and with an antibody against α-tubulin to label the cytoplasm (in green). SKI3 is detected in both the cytoplasm and the nucleus but appears to be excluded from the nucleolus. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small><strong> Figure 2. Immunofluorescence with the Diagenode antibody directed against SKI3 </strong><br />HeLa cells were stained with the Diagenode antibody against SKI3 (Cat. No. C15410011) (in red), with DAPI to label the DNA (in blue), and with an antibody against α-tubulin to label the cytoplasm (in green). SKI3 is detected in both the cytoplasm and the nucleus but appears to be excluded from the nucleolus. </small></p>
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<p><span>Polyclonal antibody raised in rabbit against human SKI3, using a recombinant protein.</span></p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410011_fig1.png" alt="Ski3 Antibody validated in Western blot" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 1. Western blot analysis using the Diagenode antibody directed against SKI3 </strong><br />Western blot was performed on HeLa nuclear extracts (lane 1) and on the purified human SKI complex (lane 2) (Figure 1A), or on nuclear extracts from HeLa cells (HeLa NE, 20 µg) (Figure 1B) using the Diagenode antibody against SKI3 (Cat. No. C15410011) diluted 1:100. The location of the protein is indicated on the right, the marker (in kDa) is shown on the left. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410011_fig2.png" alt="Ski3 Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong> Figure 2. Immunofluorescence with the Diagenode antibody directed against SKI3 </strong><br />HeLa cells were stained with the Diagenode antibody against SKI3 (Cat. No. C15410011) (in red), with DAPI to label the DNA (in blue), and with an antibody against α-tubulin to label the cytoplasm (in green). SKI3 is detected in both the cytoplasm and the nucleus but appears to be excluded from the nucleolus. </small></p>
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'info2' => '<p>SKI3 is a component of the SKI complex which is composed of SKI2, SKI3 and SKI8. The SKI complex interacts with SKI7, making the link between the SKI complex and the exosome in order to perform mRNA degradation.</p>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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