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Lot | 001 |
---|---|
Concentration | 1 μg/μl |
Species reactivity | Human |
Type | Polyclonal |
Purity | Affinity purified |
Host | Rabbit |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Applications | Suggested dilution | References |
---|---|---|
Western Blotting | 1:2,000 | Fig 1 |
Immunohistochemistry | 1:400 | Fig 2 |
Figure 1. Western blot analysis using the Diagenode antibody directed against SMAD3S423/425p
Lysates from different cells treated with TGFb (+) or untreated control cells (-) were analysed by Western blot using the Diagenode antibody against SMAD3S423/425p (cat. No. C15410327) diluted 1:2,000. The position of the phosphorylated SMAD3 is indicated on the right. Figure 1 clearly shows the antibody recognizes the phopsphorylated SMAD3 whereas it has no reactivity towards the unphosphorylated SMAD3.
Figure 2. Immunohistochemistry using the Diagenode antibody directed against SMAD3S423/425p
Formalin fixed paraffin embedded epidermal keratinocytes were stained with the Diagenode antibody against SMAD3S423/425p (cat. No. C15410327) diluted 1:400, followed by a peroxidise labelled anti-rabbit antibody. Figure 2 shows nuclear staining of SMAD3 (red).
SMAD3S423/425p polyclonal antibody DATASHEET Polyclonal antibody raised in rabbit against SMAD3 (SMAD Family Member 3) phosphorylated at Ser42... | Download |
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
SMAD3S423/425p Antibody SDS US en | Download |
SMAD3S423/425p Antibody SDS GB en | Download |
SMAD3S423/425p Antibody SDS BE fr | Download |
SMAD3S423/425p Antibody SDS FR fr | Download |
SMAD3S423/425p Antibody SDS ES es | Download |
SMAD3S423/425p Antibody SDS DE de | Download |
SMAD3S423/425p Antibody SDS JP ja | Download |
SMAD3S423/425p Antibody SDS BE nl | Download |
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No. C15410327) diluted 1:400, followed by a peroxidise labelled anti-rabbit antibody. Figure 2 shows nuclear staining of SMAD3 (red).</small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<div data-canvas-width="834.8565000000004" style="left: 94.4882px; top: 796.149px; font-size: 15px; font-family: sans-serif; transform: scaleX(1.00103);">SMAD3 (UniProt/Swiss-Prot entry P84022) belongs to the SMAD (‘mothers against decapentaplegic) family of proteins which are</div> <div data-canvas-width="834.8565000000006" style="left: 94.4882px; top: 816.144px; font-size: 15px; font-family: sans-serif; transform: scaleX(1.01195);">signal transducers and transcriptional modulators involved in multiple pathways. 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$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => 'Polyclonal antibody,peptide,N-terminus,NFYA polyclonal antibody', 'meta_description' => 'Polyclonal antibody raised in rabbit against SMAD3 (SMAD Family Member 3) phosphorylated at Ser423 and Ser425, using a KLH conjugated synthetic peptide.', 'meta_title' => 'SMAD3S423/425p polyclonal antibody - Classic | Diagenode', 'product' => array( 'Product' => array( 'id' => '2819', 'antibody_id' => '482', 'name' => 'SMAD3S423/425p polyclonal antibody ', 'description' => '<p>Alternative names: <strong>MADH3</strong>, <strong>JV15-2</strong>, <strong>HMAD-3</strong>, <strong>HSMAD3</strong>, <strong>MAD3</strong>, <strong>LDS3</strong>, <strong>HSPC193</strong>, <strong>SMAD 3</strong></p> <p>Polyclonal antibody raised in rabbit against SMAD3 (SMAD Family Member 3) phosphorylated at Ser423 and Ser425, using a KLH conjugated synthetic peptide.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410327-wb.jpg" alt="WB" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against SMAD3S423/425p</strong><br />Lysates from different cells treated with TGFb (+) or untreated control cells (-) were analysed by Western blot using the Diagenode antibody against SMAD3S423/425p (cat. No. C15410327) diluted 1:2,000. The position of the phosphorylated SMAD3 is indicated on the right. Figure 1 clearly shows the antibody recognizes the phopsphorylated SMAD3 whereas it has no reactivity towards the unphosphorylated SMAD3.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410327-ihc.jpg" alt="IHC" /></p> </div> <div class="small-9 columns"> <p><small> <strong>Figure 2. Immunohistochemistry using the Diagenode antibody directed against SMAD3S423/425p</strong><br />Formalin fixed paraffin embedded epidermal keratinocytes were stained with the Diagenode antibody against SMAD3S423/425p (cat. No. C15410327) diluted 1:400, followed by a peroxidise labelled anti-rabbit antibody. Figure 2 shows nuclear staining of SMAD3 (red).</small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<div data-canvas-width="834.8565000000004" style="left: 94.4882px; top: 796.149px; font-size: 15px; font-family: sans-serif; transform: scaleX(1.00103);">SMAD3 (UniProt/Swiss-Prot entry P84022) belongs to the SMAD (‘mothers against decapentaplegic) family of proteins which are</div> <div data-canvas-width="834.8565000000006" style="left: 94.4882px; top: 816.144px; font-size: 15px; font-family: sans-serif; transform: scaleX(1.01195);">signal transducers and transcriptional modulators involved in multiple pathways. 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It is also thought to play a role in carcinogenesis.</div>', 'label3' => '', 'info3' => '', 'format' => '100 μg/ 100 μl', 'catalog_number' => 'C15410327', 'old_catalog_number' => '', 'sf_code' => 'C15410327-D001-000526', 'type' => 'FRE', 'search_order' => '', 'price_EUR' => '435', 'price_USD' => '440', 'price_GBP' => '395', 'price_JPY' => '68145', 'price_CNY' => '', 'price_AUD' => '1100', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'smad3s423-425p-polyclonal-antibody-100-ul', 'meta_title' => 'SMAD3S423/425p polyclonal antibody - Classic | Diagenode', 'meta_keywords' => 'Polyclonal antibody,peptide,N-terminus,NFYA polyclonal antibody', 'meta_description' => 'Polyclonal antibody raised in rabbit against SMAD3 (SMAD Family Member 3) phosphorylated at Ser423 and Ser425, using a KLH conjugated synthetic peptide.', 'modified' => '2022-01-05 16:03:43', 'created' => '2016-06-22 14:09:01', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '482', 'name' => 'SMAD3S423/425p polyclonal antibody', 'description' => 'SMAD3 (UniProt/Swiss-Prot entry P84022) belongs to the SMAD (‘mothers against decapentaplegic) family of proteins which are signal transducers and transcriptional modulators involved in multiple pathways. 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Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2016-08-03 16:42:27', 'created' => '2016-06-22 14:06:36', 'select_label' => '482 - SMAD3S423/425p polyclonal antibody (001 - 1 μg/μl - Human - Affinity purified - Rabbit)' ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ) ) $language = 'jp' $meta_keywords = 'Polyclonal antibody,peptide,N-terminus,NFYA polyclonal antibody' $meta_description = 'Polyclonal antibody raised in rabbit against SMAD3 (SMAD Family Member 3) phosphorylated at Ser423 and Ser425, using a KLH conjugated synthetic peptide.' $meta_title = 'SMAD3S423/425p polyclonal antibody - Classic | Diagenode' $product = array( 'Product' => array( 'id' => '2819', 'antibody_id' => '482', 'name' => 'SMAD3S423/425p polyclonal antibody ', 'description' => '<p>Alternative names: <strong>MADH3</strong>, <strong>JV15-2</strong>, <strong>HMAD-3</strong>, <strong>HSMAD3</strong>, <strong>MAD3</strong>, <strong>LDS3</strong>, <strong>HSPC193</strong>, <strong>SMAD 3</strong></p> <p>Polyclonal antibody raised in rabbit against SMAD3 (SMAD Family Member 3) phosphorylated at Ser423 and Ser425, using a KLH conjugated synthetic peptide.</p>', 'label1' => 'Validation data', 'info1' => '<div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410327-wb.jpg" alt="WB" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 1. Western blot analysis using the Diagenode antibody directed against SMAD3S423/425p</strong><br />Lysates from different cells treated with TGFb (+) or untreated control cells (-) were analysed by Western blot using the Diagenode antibody against SMAD3S423/425p (cat. No. C15410327) diluted 1:2,000. The position of the phosphorylated SMAD3 is indicated on the right. Figure 1 clearly shows the antibody recognizes the phopsphorylated SMAD3 whereas it has no reactivity towards the unphosphorylated SMAD3.</small></p> </div> </div> <div class="row"> <div class="small-3 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410327-ihc.jpg" alt="IHC" /></p> </div> <div class="small-9 columns"> <p><small> <strong>Figure 2. Immunohistochemistry using the Diagenode antibody directed against SMAD3S423/425p</strong><br />Formalin fixed paraffin embedded epidermal keratinocytes were stained with the Diagenode antibody against SMAD3S423/425p (cat. No. C15410327) diluted 1:400, followed by a peroxidise labelled anti-rabbit antibody. Figure 2 shows nuclear staining of SMAD3 (red).</small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<div data-canvas-width="834.8565000000004" style="left: 94.4882px; top: 796.149px; font-size: 15px; font-family: sans-serif; transform: scaleX(1.00103);">SMAD3 (UniProt/Swiss-Prot entry P84022) belongs to the SMAD (‘mothers against decapentaplegic) family of proteins which are</div> <div data-canvas-width="834.8565000000006" style="left: 94.4882px; top: 816.144px; font-size: 15px; font-family: sans-serif; transform: scaleX(1.01195);">signal transducers and transcriptional modulators involved in multiple pathways. 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No. C15410327) diluted 1:400, followed by a peroxidise labelled anti-rabbit antibody. Figure 2 shows nuclear staining of SMAD3 (red).</small></p> </div> </div>', 'label2' => 'Target description', 'info2' => '<div data-canvas-width="834.8565000000004" style="left: 94.4882px; top: 796.149px; font-size: 15px; font-family: sans-serif; transform: scaleX(1.00103);">SMAD3 (UniProt/Swiss-Prot entry P84022) belongs to the SMAD (‘mothers against decapentaplegic) family of proteins which are</div> <div data-canvas-width="834.8565000000006" style="left: 94.4882px; top: 816.144px; font-size: 15px; font-family: sans-serif; transform: scaleX(1.01195);">signal transducers and transcriptional modulators involved in multiple pathways. 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