Alternative names: EVI16
Polyclonal antibody raised in rabbit against human SOX4 (SRY (sex determining region Y)-box 4), using two KLH-conjugated synthetic peptides containing a sequence from the N-terminus and from the C-terminus of the protein, respectively.
| Lot | A574-004 |
|---|---|
| Concentration | Not determined |
| Species reactivity | Human, mouse: positive. Other species: not tested. |
| Type | Polyclonal |
| Purity | Whole antiserum |
| Host | Rabbit |
| Storage Conditions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Whole antiserum from rabbit containing 0.05% azide. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:100 - 1:1,000 | Fig 1 |
| Western Blotting | 1:4,000 | Fig 2, 3 |
| IP | 6 µl/IP | Fig 4 |
Figure 1. Determination of the titer
To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against human SOX4 (Cat. No. C15310129). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:18,000.
Figure 2. Western blot analysis using the Diagenode antibody directed against SOX4
A. H1299 lung carcinoma cells were transfected with HA-tagged SOX4 (lane 1) or with a HA-tagged truncated SOX4 (395X mutant, lane 2). Whole cell extracts (60 - 100 µg) were analysed by Western blot using the Diagenode antibody against SOX4 (Cat. No. C15310129) diluted 1:4,000 in TBS-Tween containing 2% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
B. Whole cell extracts of 4 different lung carcinoma cell lines were analysed by Western blot with the Diagenode antibody against SOX4 as described above. The H522 cells are known to overexpress SOX4. Western blot performed by Montse Sanchez-Cespedes and Sandra Castillo Diez, Catalan Institute of Oncology, Barcelona, Spain.
Figure 3. Western blot analysis using the Diagenode antibody directed against SOX4
Western blot was performed on Protein extracts (~40 µg) from mouse B16-OVA (A), CT-2A (B), GL261 (C) or B16A (D) cells using the Diagenode antibody against SOX4 (Cat. No. C15310129). The cells were transfected with a SOX4 specific crRNA (lanes 2) or with a negative control crRNA (lanes 1). The antibody was diluted 1:4,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right. The lower panel shows the WB results with a GAPDH antibody used as loading control. Western blot performed by Anže Godicelj, Dana Farber Cancer Institute, Boston, USA.
Figure 4. IP using the Diagenode antibody directed against SOX4
IP was performed overnight on protein extracts (600 µg) from CT-2A (A) and GL261 (B) cells using 6 µl of the Diagenode antibody against SOX4 (Cat. No. C15310129). The immunoprecipitated proteins were subsequently analysed by Western blot with the antibody as described above. Lane 2 shows the result of the IP; the input is shown in lane 1.
 How to properly cite our product/service in your workWe strongly recommend using this: SOX4 polyclonal antibody (sample size) (Hologic Diagenode Cat# C15310129-20 Lot# A574-004 ). Click here to copy to clipboard. Using our products or services in your publication? Let us know! |
MiR-129-5p exerts Wnt signaling-dependent tumor-suppressive functionsin hepatocellular carcinoma by directly targeting hepatoma-derived growthfactor HDGF. |
Differential Expression of Members of SOX Family of Transcription Factorsin Failing Human Hearts |
Global analysis of histone modifications and long-range chromatin interactions revealed the differential cistrome changes and novel transcriptional players in human dilated cardiomyopathy. |
SOX4 can redirect TGF-β-mediated SMAD3-transcriptional output in a context-dependent manner to promote tumorigenesis. |
SOX4 inhibits oligodendrocyte differentiation of embryonic neural stem cells in vitro by inducing Hes5 expression |
TRPM7 controls mesenchymal features of breast cancer cells by tensional regulation of SOX4. |
Cancer cell specific inhibition of Wnt/β-catenin signaling by forced intracellular acidification. |
Sox4 Expression Confers Bladder Cancer Stem Cell Properties and Predicts for Poor Patient Outcome |
Novel transcriptional targets of the SRY-HMG box transcription factor SOX4 link its expression to the development of small cell lung cancer. |
