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SUV39H1 polyclonal antibody

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

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Antibodies

Catalog Number

Format

Price

C15410368

50 µg

$380.00
Bulk order

Other format

C15410368-10

10 µg

Other names: KMT1A, MG44

Polyclonal antibody raised in rabbit against human SUV39H1 (Suppressor Of Variegation 3-9 Homolog 1), using two synthetic peptides containing a sequence from the central part and the C-terminus of the protein, respectively.

Lot	A2892-0011
Concentration	2.3 µg/µl
Species reactivity	Human: positive
Type	Polyclonal ChIP-seq Grade
Purity	Affinity purified polyclonal antibody
Host	Rabbit
Storage Conditions	Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage Buffer	PBS containing 0.05% azide
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ChIP/ChIP-seq ^*	2 - 5 µg per ChIP	Fig 1, 2
ELISA	1:10,000 - 1:100,000	Fig 3
Western Blotting	1:500	Fig 4

*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1 - 5 µg per IP.

Validation data

Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1
ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

A.

B.

C.

D.

E.

Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1
ChIP was performed with 5 µg of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).

Figure 3. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be >1:1,000,000.

Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1
Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Target Description
SUV39H1 (UniProtKB/Swiss-Prot entry O43463) is a histone methyltransferase that specifically trimethylates lysine 9 of histone H3, thereby playing an essential role in transcriptional gene silencing and heterochromatin formation as well as many other regulatory processes. It’s also a component of the eNoSC (energy-dependent nucleolar silencing) complex, which mediates silencing of rDNA in response to changes in the intracellular energy status. Loss of function may cause chromosome instability. SUV39H1 is also able to methylate histone H1.

実験手法

ChIP-seq
検出方法（ChIP-seq）としてハイスループットシーケンシングと結合したクロマチン免疫沈降（ChIP）は、今やエピゲノム研究、すなわちゲノム規模でのタンパク質-DNA相互作用の研究において主要な方法の1つされています。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、および遺伝子発現に対するヒストン修飾の効果を含む様々なライフサイエンス分野で使用されています。 ChIP-seqワークフロークロマチン調製 : DNAへのヒストンまたは転... Read more

ELISA
Enzyme-linked immunosorbent assay. Read more

WB
Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more

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<p><span lang="EN-GB">Polyclonal antibody raised in rabbit against human SUV39H1 (Suppressor Of Variegation 3-9 Homolog 1), using two synthetic peptides containing a sequence from the central part and the C-terminus of the protein, respectively.</span></p>
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<p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2b-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq" /></p>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 &micro;g of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the &ldquo;iDeal ChIP-seq&rdquo; kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
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<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be &gt;1:1,000,000.</p>
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<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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<p><span lang="EN-GB">Polyclonal antibody raised in rabbit against human SUV39H1 (Suppressor Of Variegation 3-9 Homolog 1), using two synthetic peptides containing a sequence from the central part and the C-terminus of the protein, respectively.</span></p>
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<p><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 &micro;g of antibody per ChIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 &micro;g of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the &ldquo;iDeal ChIP-seq&rdquo; kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig3-elisa.png" alt="SUV39H1 Antibody ELISA Validation " /></center></div>
<div class="small-6 columns">
<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be &gt;1:1,000,000.</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig4-wb.jpg" alt="SUV39H1 Antibody validated in Western Blot" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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		'description' => 'SUV39H1 (UniProtKB/Swiss-Prot entry O43463) is a histone methyltransferase that specifically trimethylates lysine 9 of histone H3, thereby playing an essential role in transcriptional gene silencing and heterochromatin formation as well as many other regulatory processes. It’s also a component of the eNoSC (energy-dependent nucleolar silencing) complex, which mediates silencing of rDNA in response to changes in the intracellular energy status. Loss of function may cause chromosome instability. SUV39H1 is also able to methylate histone H1.',
		'clonality' => '',
		'isotype' => '',
		'lot' => 'A2892-0011',
		'concentration' => '2.3 µg/µl',
		'reactivity' => 'Human: positive',
		'type' => 'Polyclonal <strong>ChIP-seq Grade</strong>',
		'purity' => 'Affinity purified polyclonal antibody',
		'classification' => '',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 - 5 &micro;g per ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:10,000 - 1:100,000</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:500</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1 - 5 &micro;g per IP.</small></p>',
		'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
		'storage_buffer' => 'PBS containing 0.05% azide',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
		'uniprot_acc' => '',
		'slug' => 'suv39h1-polyclonal-antibody',
		'meta_keywords' => '',
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		'modified' => '2020-10-02 11:33:07',
		'created' => '2020-08-20 12:15:04',
		'select_label' => '569 - SUV39H1 antibody - ChIP-seq grade (A2892-0011 - 2.3 µg/µl - Human: positive - Affinity purified polyclonal antibody - Rabbit)'
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			'created' => '2020-09-10 15:22:29'
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			'id' => '3094',
			'antibody_id' => '569',
			'name' => 'SUV39H1 Antibody',
			'description' => '<p><strong>Other names:&nbsp;</strong><span lang="EN-GB">KMT1A, MG44</span></p>
<p><span lang="EN-GB">Polyclonal antibody raised in rabbit against human <strong>SUV39H1 (Suppressor Of Variegation 3-9 Homolog 1),</strong> using two synthetic peptides containing a sequence from the central part and the C-terminus of the protein, respectively.</span></p>',
			'label1' => 'Validation data',
			'info1' => '<div class="row">
<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig1-chip.png" alt="SUV39H1 Antibody ChIP Grade" /></center></div>
<div class="small-6 columns">
<p><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 &micro;g of antibody per ChIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
</div>
</div>
<div class="extra-spaced"></div>
<div class="row">
<div class="small-12 columns">
<p>A. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2a-chip-seq.jpg" alt="SUV39H1 Antibody Chip-seq Grade " /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2b-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2c-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq assay" /></p>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<p>D. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2d-chip-seq.jpg" alt="SUV39H1 Antibody validated in Chip-seq " /></p>
<p>E. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2e-chip-seq.jpg" alt="SUV39H1 Antibody Chip-seq Grade " /></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 &micro;g of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the &ldquo;iDeal ChIP-seq&rdquo; kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
</div>
</div>
<div class="extra-spaced"></div>
<div class="row">
<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig3-elisa.png" alt="SUV39H1 Antibody ELISA Validation " /></center></div>
<div class="small-6 columns">
<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be &gt;1:1,000,000.</p>
</div>
</div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="row">
<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig4-wb.jpg" alt="SUV39H1 Antibody validated in Western Blot" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
</div>
</div>
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<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'format' => '50 µg',
			'catalog_number' => 'C15410368',
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			'sf_code' => 'C15410368-D001-000581',
			'type' => 'FRE',
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			'price_EUR' => '380',
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			'price_GBP' => '340',
			'price_JPY' => '59525',
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			'meta_description' => 'SUV39H1 Antibody  validated  in ChIP-seq, ChIP-qPCR, ELISA and WB. Batch-specific data available on the website. Other names: KMT1A, MG44. Sample size available.',
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<div class="large-12 columns">Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomics researchers, namely to investigate protein-DNA interaction on a genome-wide scale. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</div>
<div class="large-12 columns"></div>
<h5 class="large-12 columns"><strong></strong></h5>
<h5 class="large-12 columns"><strong>The ChIP-seq workflow</strong></h5>
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-seq-diagram.png" /></div>
<div class="large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation: </strong>Crosslink&nbsp;chromatin-bound proteins (histones or transcription factors) to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing:</strong> Fragment chromatin by&nbsp;sonication to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: Capture protein-DNA complexes with&nbsp;<strong><a href="../categories/chip-seq-grade-antibodies">specific ChIP-seq grade antibodies</a></strong>&nbsp;against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: Reverse&nbsp;cross-links, elute, and purify&nbsp;</li>
<li class="large-12 columns"><strong>NGS Library Preparation</strong>: Ligate adapters and amplify&nbsp;IP'd material</li>
<li class="large-12 columns"><strong>Bioinformatic analysis</strong>: Perform r<span style="font-weight: 400;">ead filtering and trimming</span>, r<span style="font-weight: 400;">ead specific alignment, enrichment specific peak calling, QC metrics, multi-sample cross-comparison etc. </span></li>
</ol>
</div>
</div>
<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img alt="" src="https://www.diagenode.com/img/banners/banner-decide.png" /></a></div>
<div class="small-6 medium-6 large-6 columns"><a href="../pages/chip-kit-customizer-1"><img alt="" src="https://www.diagenode.com/img/banners/banner-customizer.png" /></a></div>
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			'meta_title' => 'Chromatin Immunoprecipitation - ChIP-seq Kits - Dna methylation  | Diagenode',
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<div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div>
</div>',
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			'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode',
			'modified' => '2016-01-13 12:21:41',
			'created' => '2014-07-08 08:13:28',
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			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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			'name' => 'All antibodies',
			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'name' => 'ChIP-seq grade antibodies',
			'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
</div>
<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results',
			'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode',
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			'name' => 'ChIP-grade antibodies',
			'description' => '<div class="row">
<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
</div>
<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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			'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><span lang="EN-GB">Polyclonal antibody raised in rabbit against human SUV39H1 (Suppressor Of Variegation 3-9 Homolog 1), using two synthetic peptides containing a sequence from the central part and the C-terminus of the protein, respectively.</span></p>
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<p><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 &micro;g of antibody per ChIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2b-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2c-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq assay" /></p>
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<p>D. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2d-chip-seq.jpg" alt="SUV39H1 Antibody validated in Chip-seq " /></p>
<p>E. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2e-chip-seq.jpg" alt="SUV39H1 Antibody Chip-seq Grade " /></p>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 &micro;g of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the &ldquo;iDeal ChIP-seq&rdquo; kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
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<div class="small-6 columns">
<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be &gt;1:1,000,000.</p>
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<div class="small-8 columns">
<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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<p><span lang="EN-GB">Polyclonal antibody raised in rabbit against human SUV39H1 (Suppressor Of Variegation 3-9 Homolog 1), using two synthetic peptides containing a sequence from the central part and the C-terminus of the protein, respectively.</span></p>
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<p><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 &micro;g of antibody per ChIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 &micro;g of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the &ldquo;iDeal ChIP-seq&rdquo; kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
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<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be &gt;1:1,000,000.</p>
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<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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<p><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 &micro;g of antibody per ChIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2b-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq" /></p>
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<div class="extra-spaced"></div>
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<p>D. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2d-chip-seq.jpg" alt="SUV39H1 Antibody validated in Chip-seq " /></p>
<p>E. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2e-chip-seq.jpg" alt="SUV39H1 Antibody Chip-seq Grade " /></p>
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<div class="small-12 columns">
<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 &micro;g of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the &ldquo;iDeal ChIP-seq&rdquo; kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
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<div class="row">
<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig3-elisa.png" alt="SUV39H1 Antibody ELISA Validation " /></center></div>
<div class="small-6 columns">
<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be &gt;1:1,000,000.</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig4-wb.jpg" alt="SUV39H1 Antibody validated in Western Blot" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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<div class="large-12 columns">Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomics researchers, namely to investigate protein-DNA interaction on a genome-wide scale. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</div>
<div class="large-12 columns"></div>
<h5 class="large-12 columns"><strong></strong></h5>
<h5 class="large-12 columns"><strong>The ChIP-seq workflow</strong></h5>
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-seq-diagram.png" /></div>
<div class="large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation: </strong>Crosslink&nbsp;chromatin-bound proteins (histones or transcription factors) to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing:</strong> Fragment chromatin by&nbsp;sonication to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: Capture protein-DNA complexes with&nbsp;<strong><a href="../categories/chip-seq-grade-antibodies">specific ChIP-seq grade antibodies</a></strong>&nbsp;against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: Reverse&nbsp;cross-links, elute, and purify&nbsp;</li>
<li class="large-12 columns"><strong>NGS Library Preparation</strong>: Ligate adapters and amplify&nbsp;IP'd material</li>
<li class="large-12 columns"><strong>Bioinformatic analysis</strong>: Perform r<span style="font-weight: 400;">ead filtering and trimming</span>, r<span style="font-weight: 400;">ead specific alignment, enrichment specific peak calling, QC metrics, multi-sample cross-comparison etc. </span></li>
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<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
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<div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img alt="" src="https://www.diagenode.com/img/banners/banner-decide.png" /></a></div>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
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<li>Expert technical support</li>
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<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode&rsquo;s highly validated antibodies:</p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
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<p></p>
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<p></p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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			'name' => 'SUV39H1 polyclonal antibody',
			'description' => '<p><strong>Other names:&nbsp;</strong><span lang="EN-GB">KMT1A, MG44</span></p>
<p><span lang="EN-GB">Polyclonal antibody raised in rabbit against human SUV39H1 (Suppressor Of Variegation 3-9 Homolog 1), using two synthetic peptides containing a sequence from the central part and the C-terminus of the protein, respectively.</span></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
			'label1' => 'Validation data',
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig1-chip.png" alt="SUV39H1 Antibody ChIP Grade" /></center></div>
<div class="small-6 columns">
<p><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 &micro;g of antibody per ChIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<div class="row">
<div class="small-12 columns">
<p>A. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2a-chip-seq.jpg" alt="SUV39H1 Antibody Chip-seq Grade " /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2b-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2c-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq assay" /></p>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<p>D. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2d-chip-seq.jpg" alt="SUV39H1 Antibody validated in Chip-seq " /></p>
<p>E. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2e-chip-seq.jpg" alt="SUV39H1 Antibody Chip-seq Grade " /></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 &micro;g of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the &ldquo;iDeal ChIP-seq&rdquo; kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig3-elisa.png" alt="SUV39H1 Antibody ELISA Validation " /></center></div>
<div class="small-6 columns">
<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be &gt;1:1,000,000.</p>
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<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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			'clonality' => '',
			'isotype' => '',
			'lot' => 'A2892-0011',
			'concentration' => '2.3 µg/µl',
			'reactivity' => 'Human: positive',
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			'purity' => 'Affinity purified polyclonal antibody',
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			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
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<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 - 5 &micro;g per ChIP</td>
<td>Fig 1, 2</td>
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<tr>
<td>ELISA</td>
<td>1:10,000 - 1:100,000</td>
<td>Fig 3</td>
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<tr>
<td>Western Blotting</td>
<td>1:500</td>
<td>Fig 4</td>
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<p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1 - 5 &micro;g per IP.</small></p>',
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<p><span lang="EN-GB">Polyclonal antibody raised in rabbit against human SUV39H1 (Suppressor Of Variegation 3-9 Homolog 1), using two synthetic peptides containing a sequence from the central part and the C-terminus of the protein, respectively.</span></p>
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<p><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 &micro;g of antibody per ChIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 &micro;g of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the &ldquo;iDeal ChIP-seq&rdquo; kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
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<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be &gt;1:1,000,000.</p>
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<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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<p><span lang="EN-GB">Polyclonal antibody raised in rabbit against human <strong>SUV39H1 (Suppressor Of Variegation 3-9 Homolog 1),</strong> using two synthetic peptides containing a sequence from the central part and the C-terminus of the protein, respectively.</span></p>',
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<div class="small-6 columns">
<p><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 &micro;g of antibody per ChIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<p>A. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2a-chip-seq.jpg" alt="SUV39H1 Antibody Chip-seq Grade " /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2b-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2c-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq assay" /></p>
<div class="extra-spaced"></div>
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<div class="extra-spaced"></div>
<p>D. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2d-chip-seq.jpg" alt="SUV39H1 Antibody validated in Chip-seq " /></p>
<p>E. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2e-chip-seq.jpg" alt="SUV39H1 Antibody Chip-seq Grade " /></p>
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<div class="row">
<div class="small-12 columns">
<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 &micro;g of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the &ldquo;iDeal ChIP-seq&rdquo; kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
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<div class="small-6 columns">
<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be &gt;1:1,000,000.</p>
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<div class="small-8 columns">
<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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<div class="large-12 columns">Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomics researchers, namely to investigate protein-DNA interaction on a genome-wide scale. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</div>
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<h5 class="large-12 columns"><strong></strong></h5>
<h5 class="large-12 columns"><strong>The ChIP-seq workflow</strong></h5>
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<li class="large-12 columns"><strong>Chromatin IP</strong>: Capture protein-DNA complexes with&nbsp;<strong><a href="../categories/chip-seq-grade-antibodies">specific ChIP-seq grade antibodies</a></strong>&nbsp;against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: Reverse&nbsp;cross-links, elute, and purify&nbsp;</li>
<li class="large-12 columns"><strong>NGS Library Preparation</strong>: Ligate adapters and amplify&nbsp;IP'd material</li>
<li class="large-12 columns"><strong>Bioinformatic analysis</strong>: Perform r<span style="font-weight: 400;">ead filtering and trimming</span>, r<span style="font-weight: 400;">ead specific alignment, enrichment specific peak calling, QC metrics, multi-sample cross-comparison etc. </span></li>
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<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img alt="" src="https://www.diagenode.com/img/banners/banner-decide.png" /></a></div>
<div class="small-6 medium-6 large-6 columns"><a href="../pages/chip-kit-customizer-1"><img alt="" src="https://www.diagenode.com/img/banners/banner-customizer.png" /></a></div>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<p><span lang="EN-GB">Polyclonal antibody raised in rabbit against human SUV39H1 (Suppressor Of Variegation 3-9 Homolog 1), using two synthetic peptides containing a sequence from the central part and the C-terminus of the protein, respectively.</span></p>
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<p><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 &micro;g of antibody per ChIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2b-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq" /></p>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 &micro;g of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the &ldquo;iDeal ChIP-seq&rdquo; kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
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<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be &gt;1:1,000,000.</p>
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<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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<th>References</th>
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<td>2 - 5 &micro;g per ChIP</td>
<td>Fig 1, 2</td>
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<tr>
<td>ELISA</td>
<td>1:10,000 - 1:100,000</td>
<td>Fig 3</td>
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<tr>
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<td>Fig 4</td>
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<div class="small-6 columns">
<p><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 &micro;g of antibody per ChIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<p>D. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2d-chip-seq.jpg" alt="SUV39H1 Antibody validated in Chip-seq " /></p>
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<div class="small-12 columns">
<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 &micro;g of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the &ldquo;iDeal ChIP-seq&rdquo; kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
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<div class="small-6 columns">
<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be &gt;1:1,000,000.</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig4-wb.jpg" alt="SUV39H1 Antibody validated in Western Blot" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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		'name' => 'SUV39H1 antibody - ChIP-seq grade',
		'description' => 'SUV39H1 (UniProtKB/Swiss-Prot entry O43463) is a histone methyltransferase that specifically trimethylates lysine 9 of histone H3, thereby playing an essential role in transcriptional gene silencing and heterochromatin formation as well as many other regulatory processes. It’s also a component of the eNoSC (energy-dependent nucleolar silencing) complex, which mediates silencing of rDNA in response to changes in the intracellular energy status. Loss of function may cause chromosome instability. SUV39H1 is also able to methylate histone H1.',
		'clonality' => '',
		'isotype' => '',
		'lot' => 'A2892-0011',
		'concentration' => '2.3 µg/µl',
		'reactivity' => 'Human: positive',
		'type' => 'Polyclonal <strong>ChIP-seq Grade</strong>',
		'purity' => 'Affinity purified polyclonal antibody',
		'classification' => '',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
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<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 - 5 &micro;g per ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:10,000 - 1:100,000</td>
<td>Fig 3</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:500</td>
<td>Fig 4</td>
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</tbody>
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<p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1 - 5 &micro;g per IP.</small></p>',
		'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
		'storage_buffer' => 'PBS containing 0.05% azide',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
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			'name' => 'SUV39H1 Antibody',
			'description' => '<p><strong>Other names:&nbsp;</strong><span lang="EN-GB">KMT1A, MG44</span></p>
<p><span lang="EN-GB">Polyclonal antibody raised in rabbit against human <strong>SUV39H1 (Suppressor Of Variegation 3-9 Homolog 1),</strong> using two synthetic peptides containing a sequence from the central part and the C-terminus of the protein, respectively.</span></p>',
			'label1' => 'Validation data',
			'info1' => '<div class="row">
<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig1-chip.png" alt="SUV39H1 Antibody ChIP Grade" /></center></div>
<div class="small-6 columns">
<p><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 &micro;g of antibody per ChIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
</div>
</div>
<div class="extra-spaced"></div>
<div class="row">
<div class="small-12 columns">
<p>A. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2a-chip-seq.jpg" alt="SUV39H1 Antibody Chip-seq Grade " /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2b-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2c-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq assay" /></p>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<p>D. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2d-chip-seq.jpg" alt="SUV39H1 Antibody validated in Chip-seq " /></p>
<p>E. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2e-chip-seq.jpg" alt="SUV39H1 Antibody Chip-seq Grade " /></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 &micro;g of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the &ldquo;iDeal ChIP-seq&rdquo; kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
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<div class="extra-spaced"></div>
<div class="row">
<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig3-elisa.png" alt="SUV39H1 Antibody ELISA Validation " /></center></div>
<div class="small-6 columns">
<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be &gt;1:1,000,000.</p>
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<div class="row">
<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig4-wb.jpg" alt="SUV39H1 Antibody validated in Western Blot" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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<div class="large-12 columns">Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomics researchers, namely to investigate protein-DNA interaction on a genome-wide scale. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</div>
<div class="large-12 columns"></div>
<h5 class="large-12 columns"><strong></strong></h5>
<h5 class="large-12 columns"><strong>The ChIP-seq workflow</strong></h5>
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-seq-diagram.png" /></div>
<div class="large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation: </strong>Crosslink&nbsp;chromatin-bound proteins (histones or transcription factors) to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing:</strong> Fragment chromatin by&nbsp;sonication to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: Capture protein-DNA complexes with&nbsp;<strong><a href="../categories/chip-seq-grade-antibodies">specific ChIP-seq grade antibodies</a></strong>&nbsp;against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: Reverse&nbsp;cross-links, elute, and purify&nbsp;</li>
<li class="large-12 columns"><strong>NGS Library Preparation</strong>: Ligate adapters and amplify&nbsp;IP'd material</li>
<li class="large-12 columns"><strong>Bioinformatic analysis</strong>: Perform r<span style="font-weight: 400;">ead filtering and trimming</span>, r<span style="font-weight: 400;">ead specific alignment, enrichment specific peak calling, QC metrics, multi-sample cross-comparison etc. </span></li>
</ol>
</div>
</div>
<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
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<div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div>
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			'meta_title' => 'ELISA Antibodies - Monoclonal & Polyclonal antibody | Diagenode',
			'modified' => '2016-01-13 12:21:41',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
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			'description' => '<p><span style="font-weight: 400;">Diagenode offers the large number of antibodies raised against histone modifying enzymes. The list below includes the antibodies against enzymes like: histone deacetylases, histone demethylases, histone transferases.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'name' => 'ChIP-seq grade antibodies',
			'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
</div>
<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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SUV39H1 polyclonal antibody DATASHEET Polyclonal antibody raised in rabbit against human SUV39H1 (Suppressor Of Variegation 3-9 Homolog...	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download

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SUV39H1 polyclonal antibody

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