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Polyclonal antibody raised in rabbit against TARDBP (TAR DNA binding protein), using a recombinant protein.
| Lot | 40135 |
|---|---|
| Concentration | 1.01 μg/μl |
| Species reactivity | Human, mouse, rat |
| Type | Polyclonal |
| Purity | Affinity purified |
| Host | Rabbit |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ChIP/ChIP-seq * | 2 μg/ChIP | Fig 1, 2 |
| Western Blotting | 1:1,000 - 1:3,000 | Fig 3, 4, 5 |
| Immunoprecipitation | 2 μg/IP | Fig 6 |
| Immunofluorescence | 1:100 - 1:1,000 | Fig 7 |
| Immunohistochemistry | 1:100 - 1:1,000 | Fig 8 |
* Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.
Figure 1. ChIP results obtained with the Diagenode antibody directed against TARDBP
ChIP assays were performed using K562 cells, the Diagenode antibody against TARDBP (Cat. No. C15410266) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and RFS26 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against TARDBP
ChIP was performed on sheared chromatin from 5 million K562 cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of chromosomes 3 (fig 2A and B) and 12 (fig 2C and D), respectively.
Figure 3. Western blot analysis using the Diagenode antibody directed against TARDBP
Whole cell extracts (30 μg) from Neuro2A (lane1), GL261 (lane 2), NIH3T3 (lane 3), BCL1 (lane 4) and Raw264.7 (lane 5) cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Figure 4. Western blot analysis using the Diagenode antibody directed against TARDBP
Whole cell extracts (30 μg) from A431 (lane1), H1299 (lane 2) and HeLa (lane 3),cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Figure 5. Western blot analysis using the Diagenode antibody directed against TARDBP
Whole cell extracts (30 μg) from HeLa cells transfected with sh-TARDBP (lane1), or a mock control (lane 2) were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The lower panel shows the signal obtained with an ACTB antibody, used as a loading control.
Figure 6. Immunoprecipitation using the Diagenode antibody directed against TARDBP
Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated TARDBP protein was detected by western blot with the TARDBP antibody diluted 1:1,000. The IP with the TARDBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract).
Figure 7. Immunofluorescence with the Diagenode antibody directed against TARDBP
HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against TARDBP (Cat. C15410266) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain.
Figure 8. Immunohistochemistry using the Diagenode antibody directed against TARDBP
Formalin fixed paraffin embedded rat brain tissue (figure A) or Cal27 Xenograft (figure B) was stained with the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:500 and 1:100, respectively, followed by a peroxidase labelled goat anti-rabbit secondary antibody.
TARDBP (UniProt/Swiss-Prot entry Q13148) is a DNA and RNA-binding protein which regulates transcription and splicing. It binds to the chromosomally integrated TAR DNA from HIV-1 thereby repressing HIV-1 transcription. TARDBP is also involved in the regulation of CFTR splicing where it promotes CFTR exon 9 skipping. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis.
| IF Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more |
| IHC Immunohistochemistry Read more |
| IP Immunoprecipitation Read more |
| WB Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more |
| ChIP-seq (ab) Read more |
| ChIP-qPCR (ab) Read more |
| Datasheet TARDBP C15410266 DATASHEET Datasheet description | Download |
| Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar... | Download |
| Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e... | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: TARDBP polyclonal antibody (Diagenode Cat# C15410266-100 Lot# 40135). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'TARDBP polyclonal antibody - Classic', 'meta_title' => 'TARDBP polyclonal antibody - Classic', 'product' => array( 'Product' => array( 'id' => '2384', 'antibody_id' => '427', 'name' => 'TARDBP polyclonal antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against TARDBP (TAR DNA binding protein), using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chip.png" alt="TARDBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against TARDBP (Cat. No. C15410266) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and RFS26 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-A.png" alt="TARDBP Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-B.png" alt="TARDBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-C.png" alt="TARDBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-D.png" alt="TARDBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of chromosomes 3 (fig 2A and B) and 12 (fig 2C and D), respectively. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from Neuro2A (lane1), GL261 (lane 2), NIH3T3 (lane 3), BCL1 (lane 4) and Raw264.7 (lane 5) cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-2.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from A431 (lane1), H1299 (lane 2) and HeLa (lane 3),cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-3.png" alt="TARDBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 5. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from HeLa cells transfected with sh-TARDBP (lane1), or a mock control (lane 2) were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The lower panel shows the signal obtained with an ACTB antibody, used as a loading control. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IP.png" alt="TARDBP Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 6. Immunoprecipitation using the Diagenode antibody directed against TARDBP</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated TARDBP protein was detected by western blot with the TARDBP antibody diluted 1:1,000. The IP with the TARDBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IF.png" alt="TARDBP Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 7. Immunofluorescence with the Diagenode antibody directed against TARDBP</strong><br /> HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against TARDBP (Cat. C15410266) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IHC.png" alt="TARDBP Antibody validated in Immunohistochemistry " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 8. Immunohistochemistry using the Diagenode antibody directed against TARDBP</strong><br /> Formalin fixed paraffin embedded rat brain tissue (figure A) or Cal27 Xenograft (figure B) was stained with the Diagenode antibody against TARDBP (Cat. 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ChIP results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against TARDBP (Cat. No. C15410266) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and RFS26 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-A.png" alt="TARDBP Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-B.png" alt="TARDBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-C.png" alt="TARDBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-D.png" alt="TARDBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of chromosomes 3 (fig 2A and B) and 12 (fig 2C and D), respectively. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from Neuro2A (lane1), GL261 (lane 2), NIH3T3 (lane 3), BCL1 (lane 4) and Raw264.7 (lane 5) cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-2.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from A431 (lane1), H1299 (lane 2) and HeLa (lane 3),cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-3.png" alt="TARDBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 5. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from HeLa cells transfected with sh-TARDBP (lane1), or a mock control (lane 2) were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The lower panel shows the signal obtained with an ACTB antibody, used as a loading control. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IP.png" alt="TARDBP Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 6. Immunoprecipitation using the Diagenode antibody directed against TARDBP</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated TARDBP protein was detected by western blot with the TARDBP antibody diluted 1:1,000. The IP with the TARDBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IF.png" alt="TARDBP Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 7. Immunofluorescence with the Diagenode antibody directed against TARDBP</strong><br /> HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against TARDBP (Cat. C15410266) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IHC.png" alt="TARDBP Antibody validated in Immunohistochemistry " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 8. Immunohistochemistry using the Diagenode antibody directed against TARDBP</strong><br /> Formalin fixed paraffin embedded rat brain tissue (figure A) or Cal27 Xenograft (figure B) was stained with the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:500 and 1:100, respectively, followed by a peroxidase labelled goat anti-rabbit secondary antibody. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>TARDBP (UniProt/Swiss-Prot entry Q13148) is a DNA and RNA-binding protein which regulates transcription and splicing. It binds to the chromosomally integrated TAR DNA from HIV-1 thereby repressing HIV-1 transcription. TARDBP is also involved in the regulation of CFTR splicing where it promotes CFTR exon 9 skipping. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410266-100', 'old_catalog_number' => '', 'sf_code' => 'C15410266-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'tardbp-polyclonal-antibody-classic-100-mg', 'meta_title' => 'TARDBP polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'TARDBP polyclonal antibody - Classic', 'modified' => '2022-01-05 14:57:44', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '427', 'name' => 'TARDBP polyclonal antibody - Classic', 'description' => 'TARDBP (UniProt/Swiss-Prot entry Q13148) is a DNA and RNA-binding protein which regulates transcription and splicing. It binds to the chromosomally integrated TAR DNA from HIV-1 thereby repressing HIV-1 transcription. TARDBP is also involved in the regulation of CFTR splicing where it promotes CFTR exon 9 skipping. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis.', 'clonality' => '', 'isotype' => '', 'lot' => '40135', 'concentration' => '1.01 μg/μl', 'reactivity' => 'Human, mouse, rat', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000 - 1:3,000</td> <td>Fig 3, 4, 5</td> </tr> <tr> <td>Immunoprecipitation</td> <td>2 μg/IP</td> <td>Fig 6</td> </tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 1:1,000</td> <td>Fig 7</td> </tr> <tr> <td>Immunohistochemistry</td> <td>1:100 - 1:1,000</td> <td>Fig 8</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-09-10 13:40:41', 'created' => '2015-07-28 14:11:25', 'select_label' => '427 - TARDBP polyclonal antibody - Classic (40135 - 1.01 μg/μl - Human, mouse, rat - Affinity purified - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '77', 'name' => 'C15410266', 'product_id' => '2384', 'modified' => '2016-02-18 21:46:45', 'created' => '2016-02-18 21:46:45' ) ), 'Group' => array( 'Group' => array( 'id' => '77', 'name' => 'C15410266', 'product_id' => '2384', 'modified' => '2016-02-18 21:46:45', 'created' => '2016-02-18 21:46:45' ), 'Master' => array( 'id' => '2384', 'antibody_id' => '427', 'name' => 'TARDBP Antibody', 'description' => '<p><span>Polyclonal antibody raised in rabbit against <strong>TARDBP (TAR DNA binding protein),</strong> using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chip.png" alt="TARDBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against TARDBP (Cat. No. C15410266) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and RFS26 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-A.png" alt="TARDBP Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-B.png" alt="TARDBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-C.png" alt="TARDBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-D.png" alt="TARDBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of chromosomes 3 (fig 2A and B) and 12 (fig 2C and D), respectively. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from Neuro2A (lane1), GL261 (lane 2), NIH3T3 (lane 3), BCL1 (lane 4) and Raw264.7 (lane 5) cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-2.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from A431 (lane1), H1299 (lane 2) and HeLa (lane 3),cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-3.png" alt="TARDBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 5. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from HeLa cells transfected with sh-TARDBP (lane1), or a mock control (lane 2) were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The lower panel shows the signal obtained with an ACTB antibody, used as a loading control. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IP.png" alt="TARDBP Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 6. Immunoprecipitation using the Diagenode antibody directed against TARDBP</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated TARDBP protein was detected by western blot with the TARDBP antibody diluted 1:1,000. The IP with the TARDBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IF.png" alt="TARDBP Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 7. Immunofluorescence with the Diagenode antibody directed against TARDBP</strong><br /> HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against TARDBP (Cat. C15410266) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IHC.png" alt="TARDBP Antibody validated in Immunohistochemistry " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 8. Immunohistochemistry using the Diagenode antibody directed against TARDBP</strong><br /> Formalin fixed paraffin embedded rat brain tissue (figure A) or Cal27 Xenograft (figure B) was stained with the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:500 and 1:100, respectively, followed by a peroxidase labelled goat anti-rabbit secondary antibody. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>TARDBP (UniProt/Swiss-Prot entry Q13148) is a DNA and RNA-binding protein which regulates transcription and splicing. It binds to the chromosomally integrated TAR DNA from HIV-1 thereby repressing HIV-1 transcription. TARDBP is also involved in the regulation of CFTR splicing where it promotes CFTR exon 9 skipping. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410266-100', 'old_catalog_number' => '', 'sf_code' => 'C15410266-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'tardbp-polyclonal-antibody-classic-100-mg', 'meta_title' => 'TARDBP Antibody - ChIP-seq Grade (C15410266) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'TARDBP (TAR DNA binding protein) Polyclonal Antibody validated in ChIP-qPCR, ChIP-seq, IHC, IP, WB and IF. ', 'modified' => '2022-01-05 14:57:44', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '29', 'position' => '10', 'parent_id' => '40', 'name' => 'IF', 'description' => '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '32', 'position' => '10', 'parent_id' => '40', 'name' => 'IHC', 'description' => '<p>Immunohistochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunohistochemistry', 'meta_keywords' => 'immunohistochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for immunohistochemistry applications', 'meta_title' => 'Immunohistochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:53', 'created' => '2015-07-08 13:49:03', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '424', 'name' => 'Datasheet TARDBP C15410266', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_TARDBP_C15410266.pdf', 'slug' => 'datasheet-tardbp-c15410266', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. 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Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'TARDBP polyclonal antibody - Classic', 'meta_title' => 'TARDBP polyclonal antibody - Classic', 'product' => array( 'Product' => array( 'id' => '2384', 'antibody_id' => '427', 'name' => 'TARDBP polyclonal antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against TARDBP (TAR DNA binding protein), using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chip.png" alt="TARDBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against TARDBP (Cat. No. C15410266) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and RFS26 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-A.png" alt="TARDBP Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-B.png" alt="TARDBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-C.png" alt="TARDBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-D.png" alt="TARDBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of chromosomes 3 (fig 2A and B) and 12 (fig 2C and D), respectively. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from Neuro2A (lane1), GL261 (lane 2), NIH3T3 (lane 3), BCL1 (lane 4) and Raw264.7 (lane 5) cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-2.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from A431 (lane1), H1299 (lane 2) and HeLa (lane 3),cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-3.png" alt="TARDBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 5. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from HeLa cells transfected with sh-TARDBP (lane1), or a mock control (lane 2) were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The lower panel shows the signal obtained with an ACTB antibody, used as a loading control. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IP.png" alt="TARDBP Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 6. Immunoprecipitation using the Diagenode antibody directed against TARDBP</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated TARDBP protein was detected by western blot with the TARDBP antibody diluted 1:1,000. The IP with the TARDBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IF.png" alt="TARDBP Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 7. Immunofluorescence with the Diagenode antibody directed against TARDBP</strong><br /> HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against TARDBP (Cat. C15410266) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IHC.png" alt="TARDBP Antibody validated in Immunohistochemistry " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 8. Immunohistochemistry using the Diagenode antibody directed against TARDBP</strong><br /> Formalin fixed paraffin embedded rat brain tissue (figure A) or Cal27 Xenograft (figure B) was stained with the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:500 and 1:100, respectively, followed by a peroxidase labelled goat anti-rabbit secondary antibody. </small></p> </div> </div>', 'label2' => '', 'info2' => '<p>TARDBP (UniProt/Swiss-Prot entry Q13148) is a DNA and RNA-binding protein which regulates transcription and splicing. It binds to the chromosomally integrated TAR DNA from HIV-1 thereby repressing HIV-1 transcription. TARDBP is also involved in the regulation of CFTR splicing where it promotes CFTR exon 9 skipping. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410266-100', 'old_catalog_number' => '', 'sf_code' => 'C15410266-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'tardbp-polyclonal-antibody-classic-100-mg', 'meta_title' => 'TARDBP polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'TARDBP polyclonal antibody - Classic', 'modified' => '2022-01-05 14:57:44', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '427', 'name' => 'TARDBP polyclonal antibody - Classic', 'description' => 'TARDBP (UniProt/Swiss-Prot entry Q13148) is a DNA and RNA-binding protein which regulates transcription and splicing. It binds to the chromosomally integrated TAR DNA from HIV-1 thereby repressing HIV-1 transcription. TARDBP is also involved in the regulation of CFTR splicing where it promotes CFTR exon 9 skipping. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis.', 'clonality' => '', 'isotype' => '', 'lot' => '40135', 'concentration' => '1.01 μg/μl', 'reactivity' => 'Human, mouse, rat', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000 - 1:3,000</td> <td>Fig 3, 4, 5</td> </tr> <tr> <td>Immunoprecipitation</td> <td>2 μg/IP</td> <td>Fig 6</td> </tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 1:1,000</td> <td>Fig 7</td> </tr> <tr> <td>Immunohistochemistry</td> <td>1:100 - 1:1,000</td> <td>Fig 8</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-09-10 13:40:41', 'created' => '2015-07-28 14:11:25', 'select_label' => '427 - TARDBP polyclonal antibody - Classic (40135 - 1.01 μg/μl - Human, mouse, rat - Affinity purified - Rabbit)' ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), 'meta_canonical' => 'https://www.diagenode.com/jp/p/tardbp-polyclonal-antibody-classic-100-mg' ) $language = 'jp' $meta_keywords = '' $meta_description = 'TARDBP polyclonal antibody - Classic' $meta_title = 'TARDBP polyclonal antibody - Classic' $product = array( 'Product' => array( 'id' => '2384', 'antibody_id' => '427', 'name' => 'TARDBP polyclonal antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against TARDBP (TAR DNA binding protein), using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chip.png" alt="TARDBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against TARDBP (Cat. No. C15410266) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and RFS26 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-A.png" alt="TARDBP Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-B.png" alt="TARDBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-C.png" alt="TARDBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-D.png" alt="TARDBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of chromosomes 3 (fig 2A and B) and 12 (fig 2C and D), respectively. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from Neuro2A (lane1), GL261 (lane 2), NIH3T3 (lane 3), BCL1 (lane 4) and Raw264.7 (lane 5) cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-2.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from A431 (lane1), H1299 (lane 2) and HeLa (lane 3),cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-3.png" alt="TARDBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 5. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from HeLa cells transfected with sh-TARDBP (lane1), or a mock control (lane 2) were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The lower panel shows the signal obtained with an ACTB antibody, used as a loading control. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IP.png" alt="TARDBP Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 6. Immunoprecipitation using the Diagenode antibody directed against TARDBP</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated TARDBP protein was detected by western blot with the TARDBP antibody diluted 1:1,000. The IP with the TARDBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IF.png" alt="TARDBP Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 7. Immunofluorescence with the Diagenode antibody directed against TARDBP</strong><br /> HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against TARDBP (Cat. C15410266) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IHC.png" alt="TARDBP Antibody validated in Immunohistochemistry " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 8. Immunohistochemistry using the Diagenode antibody directed against TARDBP</strong><br /> Formalin fixed paraffin embedded rat brain tissue (figure A) or Cal27 Xenograft (figure B) was stained with the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:500 and 1:100, respectively, followed by a peroxidase labelled goat anti-rabbit secondary antibody. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>TARDBP (UniProt/Swiss-Prot entry Q13148) is a DNA and RNA-binding protein which regulates transcription and splicing. It binds to the chromosomally integrated TAR DNA from HIV-1 thereby repressing HIV-1 transcription. TARDBP is also involved in the regulation of CFTR splicing where it promotes CFTR exon 9 skipping. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410266-100', 'old_catalog_number' => '', 'sf_code' => 'C15410266-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'tardbp-polyclonal-antibody-classic-100-mg', 'meta_title' => 'TARDBP polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'TARDBP polyclonal antibody - Classic', 'modified' => '2022-01-05 14:57:44', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '427', 'name' => 'TARDBP polyclonal antibody - Classic', 'description' => 'TARDBP (UniProt/Swiss-Prot entry Q13148) is a DNA and RNA-binding protein which regulates transcription and splicing. It binds to the chromosomally integrated TAR DNA from HIV-1 thereby repressing HIV-1 transcription. TARDBP is also involved in the regulation of CFTR splicing where it promotes CFTR exon 9 skipping. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis.', 'clonality' => '', 'isotype' => '', 'lot' => '40135', 'concentration' => '1.01 μg/μl', 'reactivity' => 'Human, mouse, rat', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000 - 1:3,000</td> <td>Fig 3, 4, 5</td> </tr> <tr> <td>Immunoprecipitation</td> <td>2 μg/IP</td> <td>Fig 6</td> </tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 1:1,000</td> <td>Fig 7</td> </tr> <tr> <td>Immunohistochemistry</td> <td>1:100 - 1:1,000</td> <td>Fig 8</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-09-10 13:40:41', 'created' => '2015-07-28 14:11:25', 'select_label' => '427 - TARDBP polyclonal antibody - Classic (40135 - 1.01 μg/μl - Human, mouse, rat - Affinity purified - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '77', 'name' => 'C15410266', 'product_id' => '2384', 'modified' => '2016-02-18 21:46:45', 'created' => '2016-02-18 21:46:45' ) ), 'Group' => array( 'Group' => array( 'id' => '77', 'name' => 'C15410266', 'product_id' => '2384', 'modified' => '2016-02-18 21:46:45', 'created' => '2016-02-18 21:46:45' ), 'Master' => array( 'id' => '2384', 'antibody_id' => '427', 'name' => 'TARDBP Antibody', 'description' => '<p><span>Polyclonal antibody raised in rabbit against <strong>TARDBP (TAR DNA binding protein),</strong> using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chip.png" alt="TARDBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against TARDBP (Cat. No. C15410266) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and RFS26 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-A.png" alt="TARDBP Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-B.png" alt="TARDBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-C.png" alt="TARDBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-D.png" alt="TARDBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of chromosomes 3 (fig 2A and B) and 12 (fig 2C and D), respectively. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from Neuro2A (lane1), GL261 (lane 2), NIH3T3 (lane 3), BCL1 (lane 4) and Raw264.7 (lane 5) cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-2.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from A431 (lane1), H1299 (lane 2) and HeLa (lane 3),cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-3.png" alt="TARDBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 5. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from HeLa cells transfected with sh-TARDBP (lane1), or a mock control (lane 2) were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The lower panel shows the signal obtained with an ACTB antibody, used as a loading control. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IP.png" alt="TARDBP Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 6. Immunoprecipitation using the Diagenode antibody directed against TARDBP</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated TARDBP protein was detected by western blot with the TARDBP antibody diluted 1:1,000. The IP with the TARDBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IF.png" alt="TARDBP Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 7. Immunofluorescence with the Diagenode antibody directed against TARDBP</strong><br /> HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against TARDBP (Cat. C15410266) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IHC.png" alt="TARDBP Antibody validated in Immunohistochemistry " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 8. Immunohistochemistry using the Diagenode antibody directed against TARDBP</strong><br /> Formalin fixed paraffin embedded rat brain tissue (figure A) or Cal27 Xenograft (figure B) was stained with the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:500 and 1:100, respectively, followed by a peroxidase labelled goat anti-rabbit secondary antibody. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>TARDBP (UniProt/Swiss-Prot entry Q13148) is a DNA and RNA-binding protein which regulates transcription and splicing. It binds to the chromosomally integrated TAR DNA from HIV-1 thereby repressing HIV-1 transcription. TARDBP is also involved in the regulation of CFTR splicing where it promotes CFTR exon 9 skipping. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410266-100', 'old_catalog_number' => '', 'sf_code' => 'C15410266-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'tardbp-polyclonal-antibody-classic-100-mg', 'meta_title' => 'TARDBP Antibody - ChIP-seq Grade (C15410266) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'TARDBP (TAR DNA binding protein) Polyclonal Antibody validated in ChIP-qPCR, ChIP-seq, IHC, IP, WB and IF. 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No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '32', 'position' => '10', 'parent_id' => '40', 'name' => 'IHC', 'description' => '<p>Immunohistochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunohistochemistry', 'meta_keywords' => 'immunohistochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for immunohistochemistry applications', 'meta_title' => 'Immunohistochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:53', 'created' => '2015-07-08 13:49:03', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. 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Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. 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Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'TARDBP polyclonal antibody - Classic', 'meta_title' => 'TARDBP polyclonal antibody - Classic', 'product' => array( 'Product' => array( 'id' => '2384', 'antibody_id' => '427', 'name' => 'TARDBP polyclonal antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against TARDBP (TAR DNA binding protein), using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chip.png" alt="TARDBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against TARDBP (Cat. No. C15410266) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and RFS26 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-A.png" alt="TARDBP Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-B.png" alt="TARDBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-C.png" alt="TARDBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-D.png" alt="TARDBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of chromosomes 3 (fig 2A and B) and 12 (fig 2C and D), respectively. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from Neuro2A (lane1), GL261 (lane 2), NIH3T3 (lane 3), BCL1 (lane 4) and Raw264.7 (lane 5) cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-2.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from A431 (lane1), H1299 (lane 2) and HeLa (lane 3),cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-3.png" alt="TARDBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 5. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from HeLa cells transfected with sh-TARDBP (lane1), or a mock control (lane 2) were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The lower panel shows the signal obtained with an ACTB antibody, used as a loading control. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IP.png" alt="TARDBP Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 6. Immunoprecipitation using the Diagenode antibody directed against TARDBP</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated TARDBP protein was detected by western blot with the TARDBP antibody diluted 1:1,000. The IP with the TARDBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IF.png" alt="TARDBP Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 7. Immunofluorescence with the Diagenode antibody directed against TARDBP</strong><br /> HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against TARDBP (Cat. C15410266) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IHC.png" alt="TARDBP Antibody validated in Immunohistochemistry " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 8. Immunohistochemistry using the Diagenode antibody directed against TARDBP</strong><br /> Formalin fixed paraffin embedded rat brain tissue (figure A) or Cal27 Xenograft (figure B) was stained with the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:500 and 1:100, respectively, followed by a peroxidase labelled goat anti-rabbit secondary antibody. </small></p> </div> </div>', 'label2' => '', 'info2' => '<p>TARDBP (UniProt/Swiss-Prot entry Q13148) is a DNA and RNA-binding protein which regulates transcription and splicing. It binds to the chromosomally integrated TAR DNA from HIV-1 thereby repressing HIV-1 transcription. TARDBP is also involved in the regulation of CFTR splicing where it promotes CFTR exon 9 skipping. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410266-100', 'old_catalog_number' => '', 'sf_code' => 'C15410266-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'tardbp-polyclonal-antibody-classic-100-mg', 'meta_title' => 'TARDBP polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'TARDBP polyclonal antibody - Classic', 'modified' => '2022-01-05 14:57:44', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '427', 'name' => 'TARDBP polyclonal antibody - Classic', 'description' => 'TARDBP (UniProt/Swiss-Prot entry Q13148) is a DNA and RNA-binding protein which regulates transcription and splicing. It binds to the chromosomally integrated TAR DNA from HIV-1 thereby repressing HIV-1 transcription. TARDBP is also involved in the regulation of CFTR splicing where it promotes CFTR exon 9 skipping. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis.', 'clonality' => '', 'isotype' => '', 'lot' => '40135', 'concentration' => '1.01 μg/μl', 'reactivity' => 'Human, mouse, rat', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000 - 1:3,000</td> <td>Fig 3, 4, 5</td> </tr> <tr> <td>Immunoprecipitation</td> <td>2 μg/IP</td> <td>Fig 6</td> </tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 1:1,000</td> <td>Fig 7</td> </tr> <tr> <td>Immunohistochemistry</td> <td>1:100 - 1:1,000</td> <td>Fig 8</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-09-10 13:40:41', 'created' => '2015-07-28 14:11:25', 'select_label' => '427 - TARDBP polyclonal antibody - Classic (40135 - 1.01 μg/μl - Human, mouse, rat - Affinity purified - Rabbit)' ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array( (int) 0 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array(), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ), 'meta_canonical' => 'https://www.diagenode.com/jp/p/tardbp-polyclonal-antibody-classic-100-mg' ) $language = 'jp' $meta_keywords = '' $meta_description = 'TARDBP polyclonal antibody - Classic' $meta_title = 'TARDBP polyclonal antibody - Classic' $product = array( 'Product' => array( 'id' => '2384', 'antibody_id' => '427', 'name' => 'TARDBP polyclonal antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against TARDBP (TAR DNA binding protein), using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chip.png" alt="TARDBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against TARDBP (Cat. No. C15410266) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and RFS26 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-A.png" alt="TARDBP Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-B.png" alt="TARDBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-C.png" alt="TARDBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-D.png" alt="TARDBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of chromosomes 3 (fig 2A and B) and 12 (fig 2C and D), respectively. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from Neuro2A (lane1), GL261 (lane 2), NIH3T3 (lane 3), BCL1 (lane 4) and Raw264.7 (lane 5) cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-2.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from A431 (lane1), H1299 (lane 2) and HeLa (lane 3),cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-3.png" alt="TARDBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 5. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from HeLa cells transfected with sh-TARDBP (lane1), or a mock control (lane 2) were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The lower panel shows the signal obtained with an ACTB antibody, used as a loading control. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IP.png" alt="TARDBP Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 6. Immunoprecipitation using the Diagenode antibody directed against TARDBP</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated TARDBP protein was detected by western blot with the TARDBP antibody diluted 1:1,000. The IP with the TARDBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IF.png" alt="TARDBP Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 7. Immunofluorescence with the Diagenode antibody directed against TARDBP</strong><br /> HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against TARDBP (Cat. C15410266) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IHC.png" alt="TARDBP Antibody validated in Immunohistochemistry " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 8. Immunohistochemistry using the Diagenode antibody directed against TARDBP</strong><br /> Formalin fixed paraffin embedded rat brain tissue (figure A) or Cal27 Xenograft (figure B) was stained with the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:500 and 1:100, respectively, followed by a peroxidase labelled goat anti-rabbit secondary antibody. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>TARDBP (UniProt/Swiss-Prot entry Q13148) is a DNA and RNA-binding protein which regulates transcription and splicing. It binds to the chromosomally integrated TAR DNA from HIV-1 thereby repressing HIV-1 transcription. TARDBP is also involved in the regulation of CFTR splicing where it promotes CFTR exon 9 skipping. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410266-100', 'old_catalog_number' => '', 'sf_code' => 'C15410266-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'tardbp-polyclonal-antibody-classic-100-mg', 'meta_title' => 'TARDBP polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'TARDBP polyclonal antibody - Classic', 'modified' => '2022-01-05 14:57:44', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '427', 'name' => 'TARDBP polyclonal antibody - Classic', 'description' => 'TARDBP (UniProt/Swiss-Prot entry Q13148) is a DNA and RNA-binding protein which regulates transcription and splicing. It binds to the chromosomally integrated TAR DNA from HIV-1 thereby repressing HIV-1 transcription. TARDBP is also involved in the regulation of CFTR splicing where it promotes CFTR exon 9 skipping. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis.', 'clonality' => '', 'isotype' => '', 'lot' => '40135', 'concentration' => '1.01 μg/μl', 'reactivity' => 'Human, mouse, rat', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000 - 1:3,000</td> <td>Fig 3, 4, 5</td> </tr> <tr> <td>Immunoprecipitation</td> <td>2 μg/IP</td> <td>Fig 6</td> </tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 1:1,000</td> <td>Fig 7</td> </tr> <tr> <td>Immunohistochemistry</td> <td>1:100 - 1:1,000</td> <td>Fig 8</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-09-10 13:40:41', 'created' => '2015-07-28 14:11:25', 'select_label' => '427 - TARDBP polyclonal antibody - Classic (40135 - 1.01 μg/μl - Human, mouse, rat - Affinity purified - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '77', 'name' => 'C15410266', 'product_id' => '2384', 'modified' => '2016-02-18 21:46:45', 'created' => '2016-02-18 21:46:45' ) ), 'Group' => array( 'Group' => array( 'id' => '77', 'name' => 'C15410266', 'product_id' => '2384', 'modified' => '2016-02-18 21:46:45', 'created' => '2016-02-18 21:46:45' ), 'Master' => array( 'id' => '2384', 'antibody_id' => '427', 'name' => 'TARDBP Antibody', 'description' => '<p><span>Polyclonal antibody raised in rabbit against <strong>TARDBP (TAR DNA binding protein),</strong> using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chip.png" alt="TARDBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against TARDBP (Cat. No. C15410266) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and RFS26 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-A.png" alt="TARDBP Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-B.png" alt="TARDBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-C.png" alt="TARDBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-D.png" alt="TARDBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of chromosomes 3 (fig 2A and B) and 12 (fig 2C and D), respectively. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from Neuro2A (lane1), GL261 (lane 2), NIH3T3 (lane 3), BCL1 (lane 4) and Raw264.7 (lane 5) cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-2.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from A431 (lane1), H1299 (lane 2) and HeLa (lane 3),cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-3.png" alt="TARDBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 5. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from HeLa cells transfected with sh-TARDBP (lane1), or a mock control (lane 2) were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The lower panel shows the signal obtained with an ACTB antibody, used as a loading control. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IP.png" alt="TARDBP Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 6. Immunoprecipitation using the Diagenode antibody directed against TARDBP</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated TARDBP protein was detected by western blot with the TARDBP antibody diluted 1:1,000. The IP with the TARDBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IF.png" alt="TARDBP Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 7. Immunofluorescence with the Diagenode antibody directed against TARDBP</strong><br /> HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against TARDBP (Cat. C15410266) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IHC.png" alt="TARDBP Antibody validated in Immunohistochemistry " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 8. Immunohistochemistry using the Diagenode antibody directed against TARDBP</strong><br /> Formalin fixed paraffin embedded rat brain tissue (figure A) or Cal27 Xenograft (figure B) was stained with the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:500 and 1:100, respectively, followed by a peroxidase labelled goat anti-rabbit secondary antibody. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>TARDBP (UniProt/Swiss-Prot entry Q13148) is a DNA and RNA-binding protein which regulates transcription and splicing. It binds to the chromosomally integrated TAR DNA from HIV-1 thereby repressing HIV-1 transcription. TARDBP is also involved in the regulation of CFTR splicing where it promotes CFTR exon 9 skipping. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410266-100', 'old_catalog_number' => '', 'sf_code' => 'C15410266-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'tardbp-polyclonal-antibody-classic-100-mg', 'meta_title' => 'TARDBP Antibody - ChIP-seq Grade (C15410266) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'TARDBP (TAR DNA binding protein) Polyclonal Antibody validated in ChIP-qPCR, ChIP-seq, IHC, IP, WB and IF. ', 'modified' => '2022-01-05 14:57:44', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array( (int) 0 => array( 'id' => '29', 'position' => '10', 'parent_id' => '40', 'name' => 'IF', 'description' => '<p><strong>Immunofluorescence</strong>:</p> <p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p> <p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p> <p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p> <h5><sup>Check our selection of antibodies validated in IF.</sup></h5>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunofluorescence', 'meta_keywords' => 'Immunofluorescence,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunofluorescence applications', 'meta_title' => 'Immunofluorescence - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-27 16:23:10', 'created' => '2015-07-08 13:46:02', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '32', 'position' => '10', 'parent_id' => '40', 'name' => 'IHC', 'description' => '<p>Immunohistochemistry</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunohistochemistry', 'meta_keywords' => 'immunohistochemistry,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for immunohistochemistry applications', 'meta_title' => 'Immunohistochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:53', 'created' => '2015-07-08 13:49:03', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '30', 'position' => '10', 'parent_id' => '40', 'name' => 'IP', 'description' => '<p>Immunoprecipitation</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'immunoprecipitation', 'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications', 'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-01-13 12:23:07', 'created' => '2015-07-08 13:46:50', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '19', 'position' => '10', 'parent_id' => '40', 'name' => 'WB', 'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. Check the list below to see our targets.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li><span style="font-weight: 400;"> Highly sensitive and specific</span></li> <li><span style="font-weight: 400;"> Cost-effective (requires less antibody per reaction)</span></li> <li><span style="font-weight: 400;"> Batch-specific data is available on the website</span></li> <li><span style="font-weight: 400;"> Expert technical support</span></li> <li><span style="font-weight: 400;"> Sample sizes available</span></li> <li><span style="font-weight: 400;"> 100% satisfaction guarantee</span></li> </ul>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'transcription-factor', 'cookies_tag_id' => null, 'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies', 'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie', 'meta_title' => 'Transcription factor Antibodies | Diagenode', 'modified' => '2020-07-06 12:59:19', 'created' => '2015-03-12 10:20:08', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-seq grade antibodies', 'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p> <p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-grade antibodies, polyclonal antibody, monoclonal antibody, Diagenode', 'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP', 'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode', 'modified' => '2024-11-19 17:27:07', 'created' => '2017-02-14 11:16:04', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '424', 'name' => 'Datasheet TARDBP C15410266', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/antibodies/Datasheet_TARDBP_C15410266.pdf', 'slug' => 'datasheet-tardbp-c15410266', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:44', 'created' => '2015-07-07 11:47:44', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '11', 'name' => 'Antibodies you can trust', 'description' => '<p style="text-align: justify;"><span>Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of large sets of genome-wide sequencing data. ChIP sequencing (ChIP-seq) has now become the gold standard method for chromatin studies, given the accuracy and coverage scale of the approach over other methods. Successful ChIP-seq, however, requires a higher level of experimental accuracy and consistency in all steps of ChIP than ever before. Particularly crucial is the quality of ChIP antibodies. </span></p>', 'image_id' => null, 'type' => 'Poster', 'url' => 'files/posters/Antibodies_you_can_trust_Poster.pdf', 'slug' => 'antibodies-you-can-trust-poster', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-10-01 20:18:31', 'created' => '2015-07-03 16:05:15', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'Epigenetic Antibodies Brochure', 'description' => '<p>More than in any other immuoprecipitation assays, quality antibodies are critical tools in many epigenetics experiments. 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Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'TARDBP polyclonal antibody - Classic', 'meta_title' => 'TARDBP polyclonal antibody - Classic', 'product' => array( 'Product' => array( 'id' => '2384', 'antibody_id' => '427', 'name' => 'TARDBP polyclonal antibody ', 'description' => '<p><span>Polyclonal antibody raised in rabbit against TARDBP (TAR DNA binding protein), using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chip.png" alt="TARDBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against TARDBP (Cat. No. C15410266) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and RFS26 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-A.png" alt="TARDBP Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-B.png" alt="TARDBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-C.png" alt="TARDBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-D.png" alt="TARDBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of chromosomes 3 (fig 2A and B) and 12 (fig 2C and D), respectively. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from Neuro2A (lane1), GL261 (lane 2), NIH3T3 (lane 3), BCL1 (lane 4) and Raw264.7 (lane 5) cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-2.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from A431 (lane1), H1299 (lane 2) and HeLa (lane 3),cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-3.png" alt="TARDBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 5. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from HeLa cells transfected with sh-TARDBP (lane1), or a mock control (lane 2) were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The lower panel shows the signal obtained with an ACTB antibody, used as a loading control. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IP.png" alt="TARDBP Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 6. Immunoprecipitation using the Diagenode antibody directed against TARDBP</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated TARDBP protein was detected by western blot with the TARDBP antibody diluted 1:1,000. The IP with the TARDBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IF.png" alt="TARDBP Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 7. Immunofluorescence with the Diagenode antibody directed against TARDBP</strong><br /> HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against TARDBP (Cat. C15410266) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IHC.png" alt="TARDBP Antibody validated in Immunohistochemistry " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 8. Immunohistochemistry using the Diagenode antibody directed against TARDBP</strong><br /> Formalin fixed paraffin embedded rat brain tissue (figure A) or Cal27 Xenograft (figure B) was stained with the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:500 and 1:100, respectively, followed by a peroxidase labelled goat anti-rabbit secondary antibody. </small></p> </div> </div>', 'label2' => '', 'info2' => '<p>TARDBP (UniProt/Swiss-Prot entry Q13148) is a DNA and RNA-binding protein which regulates transcription and splicing. It binds to the chromosomally integrated TAR DNA from HIV-1 thereby repressing HIV-1 transcription. TARDBP is also involved in the regulation of CFTR splicing where it promotes CFTR exon 9 skipping. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410266-100', 'old_catalog_number' => '', 'sf_code' => 'C15410266-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'tardbp-polyclonal-antibody-classic-100-mg', 'meta_title' => 'TARDBP polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'TARDBP polyclonal antibody - Classic', 'modified' => '2022-01-05 14:57:44', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '427', 'name' => 'TARDBP polyclonal antibody - Classic', 'description' => 'TARDBP (UniProt/Swiss-Prot entry Q13148) is a DNA and RNA-binding protein which regulates transcription and splicing. It binds to the chromosomally integrated TAR DNA from HIV-1 thereby repressing HIV-1 transcription. TARDBP is also involved in the regulation of CFTR splicing where it promotes CFTR exon 9 skipping. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis.', 'clonality' => '', 'isotype' => '', 'lot' => '40135', 'concentration' => '1.01 μg/μl', 'reactivity' => 'Human, mouse, rat', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000 - 1:3,000</td> <td>Fig 3, 4, 5</td> </tr> <tr> <td>Immunoprecipitation</td> <td>2 μg/IP</td> <td>Fig 6</td> </tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 1:1,000</td> <td>Fig 7</td> </tr> <tr> <td>Immunohistochemistry</td> <td>1:100 - 1:1,000</td> <td>Fig 8</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. 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ChIP results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against TARDBP (Cat. No. C15410266) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and RFS26 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-A.png" alt="TARDBP Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-B.png" alt="TARDBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-C.png" alt="TARDBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-D.png" alt="TARDBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of chromosomes 3 (fig 2A and B) and 12 (fig 2C and D), respectively. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from Neuro2A (lane1), GL261 (lane 2), NIH3T3 (lane 3), BCL1 (lane 4) and Raw264.7 (lane 5) cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-2.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from A431 (lane1), H1299 (lane 2) and HeLa (lane 3),cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-3.png" alt="TARDBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 5. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from HeLa cells transfected with sh-TARDBP (lane1), or a mock control (lane 2) were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The lower panel shows the signal obtained with an ACTB antibody, used as a loading control. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IP.png" alt="TARDBP Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 6. Immunoprecipitation using the Diagenode antibody directed against TARDBP</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated TARDBP protein was detected by western blot with the TARDBP antibody diluted 1:1,000. The IP with the TARDBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IF.png" alt="TARDBP Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 7. Immunofluorescence with the Diagenode antibody directed against TARDBP</strong><br /> HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against TARDBP (Cat. C15410266) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IHC.png" alt="TARDBP Antibody validated in Immunohistochemistry " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 8. Immunohistochemistry using the Diagenode antibody directed against TARDBP</strong><br /> Formalin fixed paraffin embedded rat brain tissue (figure A) or Cal27 Xenograft (figure B) was stained with the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:500 and 1:100, respectively, followed by a peroxidase labelled goat anti-rabbit secondary antibody. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>TARDBP (UniProt/Swiss-Prot entry Q13148) is a DNA and RNA-binding protein which regulates transcription and splicing. It binds to the chromosomally integrated TAR DNA from HIV-1 thereby repressing HIV-1 transcription. TARDBP is also involved in the regulation of CFTR splicing where it promotes CFTR exon 9 skipping. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410266-100', 'old_catalog_number' => '', 'sf_code' => 'C15410266-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'tardbp-polyclonal-antibody-classic-100-mg', 'meta_title' => 'TARDBP polyclonal antibody - Classic', 'meta_keywords' => '', 'meta_description' => 'TARDBP polyclonal antibody - Classic', 'modified' => '2022-01-05 14:57:44', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => '427', 'name' => 'TARDBP polyclonal antibody - Classic', 'description' => 'TARDBP (UniProt/Swiss-Prot entry Q13148) is a DNA and RNA-binding protein which regulates transcription and splicing. It binds to the chromosomally integrated TAR DNA from HIV-1 thereby repressing HIV-1 transcription. TARDBP is also involved in the regulation of CFTR splicing where it promotes CFTR exon 9 skipping. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis.', 'clonality' => '', 'isotype' => '', 'lot' => '40135', 'concentration' => '1.01 μg/μl', 'reactivity' => 'Human, mouse, rat', 'type' => 'Polyclonal', 'purity' => 'Affinity purified', 'classification' => '', 'application_table' => '<table> <thead> <tr> <th>Applications</th> <th>Suggested dilution</th> <th>References</th> </tr> </thead> <tbody> <tr> <td>ChIP/ChIP-seq <sup>*</sup></td> <td>2 μg/ChIP</td> <td>Fig 1, 2</td> </tr> <tr> <td>Western Blotting</td> <td>1:1,000 - 1:3,000</td> <td>Fig 3, 4, 5</td> </tr> <tr> <td>Immunoprecipitation</td> <td>2 μg/IP</td> <td>Fig 6</td> </tr> <tr> <td>Immunofluorescence</td> <td>1:100 - 1:1,000</td> <td>Fig 7</td> </tr> <tr> <td>Immunohistochemistry</td> <td>1:100 - 1:1,000</td> <td>Fig 8</td> </tr> </tbody> </table> <p><small><sup>*</sup> Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>', 'storage_conditions' => '', 'storage_buffer' => '', 'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.', 'uniprot_acc' => '', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-09-10 13:40:41', 'created' => '2015-07-28 14:11:25', 'select_label' => '427 - TARDBP polyclonal antibody - Classic (40135 - 1.01 μg/μl - Human, mouse, rat - Affinity purified - Rabbit)' ), 'Slave' => array( (int) 0 => array( 'id' => '77', 'name' => 'C15410266', 'product_id' => '2384', 'modified' => '2016-02-18 21:46:45', 'created' => '2016-02-18 21:46:45' ) ), 'Group' => array( 'Group' => array( 'id' => '77', 'name' => 'C15410266', 'product_id' => '2384', 'modified' => '2016-02-18 21:46:45', 'created' => '2016-02-18 21:46:45' ), 'Master' => array( 'id' => '2384', 'antibody_id' => '427', 'name' => 'TARDBP Antibody', 'description' => '<p><span>Polyclonal antibody raised in rabbit against <strong>TARDBP (TAR DNA binding protein),</strong> using a recombinant protein.</span></p>', 'label1' => 'Validation Data', 'info1' => '<div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chip.png" alt="TARDBP Antibody ChIP Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against TARDBP (Cat. No. C15410266) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH and RFS26 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p> </div> </div> <div class="row"> <div class="small-5 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-A.png" alt="TARDBP Antibody ChIP-seq Grade" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-B.png" alt="TARDBP Antibody for ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-C.png" alt="TARDBP Antibody for ChIP-seq assay" style="display: block; margin-left: auto; margin-right: auto;" /></p> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-chipseq-D.png" alt="TARDBP Antibody validated in ChIP-seq" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-7 columns"> <p><small> <strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against TARDBP</strong><br /> ChIP was performed on sheared chromatin from 5 million K562 cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of chromosomes 3 (fig 2A and B) and 12 (fig 2C and D), respectively. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 3. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from Neuro2A (lane1), GL261 (lane 2), NIH3T3 (lane 3), BCL1 (lane 4) and Raw264.7 (lane 5) cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:3,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-2.png" alt="TARDBP Antibody validated in Western Blot " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 4. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from A431 (lane1), H1299 (lane 2) and HeLa (lane 3),cells were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-WB-3.png" alt="TARDBP Antibody validated in Western Blot" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 5. Western blot analysis using the Diagenode antibody directed against TARDBP</strong><br /> Whole cell extracts (30 μg) from HeLa cells transfected with sh-TARDBP (lane1), or a mock control (lane 2) were analysed by Western blot using the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The lower panel shows the signal obtained with an ACTB antibody, used as a loading control. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IP.png" alt="TARDBP Antibody validated in Immunoprecipitation" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 6. Immunoprecipitation using the Diagenode antibody directed against TARDBP</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 2 μg of the Diagenode antibody against TARDBP (Cat. No. C15410266). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated TARDBP protein was detected by western blot with the TARDBP antibody diluted 1:1,000. The IP with the TARDBP antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of HeLa whole cell extract). </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IF.png" alt="TARDBP Antibody validated in Immunofluorescence" style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 7. Immunofluorescence with the Diagenode antibody directed against TARDBP</strong><br /> HeLa cells were fixed with 4% formaldehyde for 15’ at room temperature and stained with the Diagenode antibody against TARDBP (Cat. C15410266) diluted 1:200 (left). The right picture shows costaining with Hoechst 33342 nucleic acid stain. </small></p> </div> </div> <div class="row"> <div class="small-4 columns"> <p><img src="https://www.diagenode.com/img/product/antibodies/C15410266-IHC.png" alt="TARDBP Antibody validated in Immunohistochemistry " style="display: block; margin-left: auto; margin-right: auto;" /></p> </div> <div class="small-8 columns"> <p><small> <strong>Figure 8. Immunohistochemistry using the Diagenode antibody directed against TARDBP</strong><br /> Formalin fixed paraffin embedded rat brain tissue (figure A) or Cal27 Xenograft (figure B) was stained with the Diagenode antibody against TARDBP (Cat. No. C15410266) diluted 1:500 and 1:100, respectively, followed by a peroxidase labelled goat anti-rabbit secondary antibody. </small></p> </div> </div>', 'label2' => 'Target Description', 'info2' => '<p>TARDBP (UniProt/Swiss-Prot entry Q13148) is a DNA and RNA-binding protein which regulates transcription and splicing. It binds to the chromosomally integrated TAR DNA from HIV-1 thereby repressing HIV-1 transcription. TARDBP is also involved in the regulation of CFTR splicing where it promotes CFTR exon 9 skipping. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis.</p>', 'label3' => '', 'info3' => '', 'format' => '100 μl', 'catalog_number' => 'C15410266-100', 'old_catalog_number' => '', 'sf_code' => 'C15410266-D001-001161', 'type' => 'FRE', 'search_order' => '03-Antibody', 'price_EUR' => '395', 'price_USD' => '410', 'price_GBP' => '345', 'price_JPY' => '61875', 'price_CNY' => '', 'price_AUD' => '1025', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'tardbp-polyclonal-antibody-classic-100-mg', 'meta_title' => 'TARDBP Antibody - ChIP-seq Grade (C15410266) | Diagenode', 'meta_keywords' => '', 'meta_description' => 'TARDBP (TAR DNA binding protein) Polyclonal Antibody validated in ChIP-qPCR, ChIP-seq, IHC, IP, WB and IF. 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Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p> <p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p> <p><em></em>Check our selection of antibodies validated in Western blot.</p>', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'western-blot-antibodies', 'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications', 'meta_title' => ' Western Blot - Monoclonal antibody - Polyclonal antibody | Diagenode', 'modified' => '2016-04-26 12:44:51', 'created' => '2015-01-07 09:20:00', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '42', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-seq (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-seq-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications', 'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode', 'modified' => '2016-01-20 11:06:19', 'created' => '2015-10-20 11:44:45', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '43', 'position' => '10', 'parent_id' => '40', 'name' => 'ChIP-qPCR (ab)', 'description' => '', 'in_footer' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr-antibodies', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications', 'meta_title' => 'ChIP Quantitative PCR Antibodies (ChIP-qPCR) | Diagenode', 'modified' => '2016-01-20 11:30:24', 'created' => '2015-10-20 11:45:36', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '30', 'position' => '50', 'parent_id' => '4', 'name' => 'Transcription', 'description' => '<p><span style="font-weight: 400;">The list of Diagenode’s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. 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Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p> <div class="row"> <div class="small-12 medium-9 large-9 columns"> <p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p> <img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div> <div class="small-12 medium-3 large-3 columns"> <p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p> </div> </div> <p>Diagenode’s highly validated antibodies:</p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'chip-seq-grade-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)', 'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results', 'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode', 'modified' => '2019-07-03 10:57:22', 'created' => '2015-02-16 02:24:01', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 2 => array( 'id' => '103', 'position' => '0', 'parent_id' => '4', 'name' => 'All antibodies', 'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p> <p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p> <ul> <li>Highly sensitive and specific</li> <li>Cost-effective (requires less antibody per reaction)</li> <li>Batch-specific data is available on the website</li> <li>Expert technical support</li> <li>Sample sizes available</li> <li>100% satisfaction guarantee</li> </ul>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => true, 'all_format' => false, 'is_antibody' => true, 'slug' => 'all-antibodies', 'cookies_tag_id' => null, 'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer', 'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies', 'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode', 'modified' => '2019-07-03 10:55:44', 'created' => '2015-11-02 14:49:22', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 3 => array( 'id' => '127', 'position' => '10', 'parent_id' => '4', 'name' => 'ChIP-grade antibodies', 'description' => '<div class="row"> <div class="small-12 columns"><center></center> <p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p> <p></p> </div> </div> <p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p> <div class="row"> <div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div> <div class="small-12 medium-6 large-6 columns"> <p></p> <p></p> <p></p> </div> </div> <p></p> <p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. 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