| iST Sample Preparation with the Bioruptor® The PreOmics iST sample preparation of proteins combined with the Diagenode Bioruptor® Pico e... | Download |
The iST cartridge holder has been especially designed for being used with the Picoruptor® sonication device and with the PreOmics iST sample preparation kits (8/96 reactions kits by PreOmics GmbH - P.O.00001/P.O.00027) and iST-NHS sample preparation kits (12/96 reactions kits by PreOmics GmbH - P.O.00026/P.O.00030). The holder is perfectly suited to process up to 20 PreOmics' cartridges, thus drastically reducing overall processing time and minimizing potential sample loss when operating with low input amounts. A convenient dock allows preparing the samples in a user-friendly manner.
TESTIMONIALWe at PreOmics are using the Bioruptor® Pico on a daily basis along with the iST kits for our protein sample processing workflows. The ultrasonication-based lysis greatly improves cell and tissue disruption, as well as shearing of DNA, generating samples with significantly increased peptide and protein identifications. The Bioruptor Pico in our lab has successfully processed several thousands of samples such as cells, mammalian and plant tissues, bacteria or yeast. We are highly satisfied with the handling and reproducibility of the instrument and recommend it to improve sample preparation for protein analysis.
Dr. Fabian Hosp, Head of Applications, PreOmics, Martinsried, Germany
| iST Sample Preparation with the Bioruptor® The PreOmics iST sample preparation of proteins combined with the Diagenode Bioruptor® Pico e... | Download |
| Increase your iST ultrasonication throughput with the new Bioruptor® Pico cartridge holder WHITE PAPER The PreOmics iST sample preparation of proteins combined with the Diagenode Bioruptor® Pico e... | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: iST cartridge holder for the Picoruptor (PreOmics) (Diagenode Cat# B01200045). Click here to copy to clipboard. Using our products in your publication? Let us know! |
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Check it now:</span></p> <center><span></span></center><center><a href="https://www.diagenode.com/p/bioruptorpico2"> <img alt="New Bioruptor Pico" src="https://www.diagenode.com/img/product/shearing_technologies/new-pico-product-banner.jpg" /></a></center> <p></p> <p><span>Watch our short video about the Bioruptor Pico and how it can help you accomplish perfect shearing for any application including chromatin shearing, DNA shearing for NGS, unmatched DNA extraction from FFPE samples, RNA shearing, protein extraction, and much more.</span></p> <p> <script>// <![CDATA[ (function(){var 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Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table style="width: 925px;"> <tbody> <tr valign="middle"> <td style="width: 213px;"></td> <td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center; 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The efficient disruption and homogenization of tissues and cultured cells obtained in just one step using <strong>Diagenode's Bioruptor</strong><sup>®</sup> deliver high quality protein.</div> </div> <p></p> <div class="row"> <div class="small-6 medium-6 large-6 columns text-center"><img src="https://www.diagenode.com/img/applications/protein_extraction_standard_plus.png" /> <p><small>Western blot analysis of GAPDH and HSP90 proteins in tissues (various mouse tissues) and cultured cell extracts (HeLA).</small></p> </div> <div class="small-6 medium-6 large-6 columns text-center"><img src="https://www.diagenode.com/img/applications/protein_extraction_pico.png" /> <p><small>Western blot analysis of GAPDH and ß-tubulin proteins in tissues (mouse liver) and cultured cell extracts (HeLA).</small></p> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'protein-extraction', 'meta_keywords' => 'Protein extraction,Bioruptor,Sonication,Protein Analysis', 'meta_description' => 'Diagenode provides efficient disruption and homogenization of tissues and cultured cells obtained in just one step using Bioruptor® deliver high quality protein.', 'meta_title' => 'Protein extraction using Bioruptor® Sonication device | Diagenode', 'modified' => '2017-10-16 14:39:42', 'created' => '2014-07-02 04:41:03', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '40', 'position' => '1', 'parent_id' => '6', 'name' => 'Bioruptor<sup>®</sup> Pico accessories', 'description' => '<p>All you need for the smooth running of the Bioruptor<sup>®</sup> Pico. Download our quick guide <a href="../documents/bioruptor-organigram-tubes">"Which tubes for which Bioruptor<sup><span>®</span></sup>?"</a> to find the best tubes for your applications.</p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'bioruptor-pico-accessories', 'cookies_tag_id' => null, 'meta_keywords' => 'Bioruptor® Pico accessories,DNA shearing ,chromatin shearing,sonication device,sonicator', 'meta_description' => 'All you need for the smooth running of the Bioruptor® Pico', 'meta_title' => 'Accessories for Bioruptor® Pico sonication device | Diagenode', 'modified' => '2018-02-14 16:24:24', 'created' => '2015-06-12 10:01:53', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '1061', 'name' => 'Increase your iST ultrasonication throughput with the new Bioruptor® Pico cartridge holder', 'description' => '<p><span>The PreOmics iST sample preparation of proteins combined with the Diagenode Bioruptor® Pico enhances mass spectrometric analyses. 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Thus, the ultrasonication-based cell lysis aids in proteome-wide discovery by greatly enhances the number of peptide and protein identifications.</span></p> <p><span><span>The <a href="https://www.diagenode.com/en/products/view/3039">new Bioruptor cartridge holder</a> allows placing of up to 20 iST cartridges directly into the <a href="https://www.diagenode.com/en/p/bioruptor-pico-sonication-device">Bioruptor Pico</a>, thus minimizing sample loss and increasing throughput.</span><br /></span></p>', 'image_id' => null, 'type' => 'White Paper', 'url' => 'files/products/shearing_technology/bioruptor_accessories/whitepaper-ist-adaptors.pdf', 'slug' => 'wp-ist-adaptators', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-10-28 16:31:40', 'created' => '2019-10-28 15:23:29', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array( (int) 0 => array( 'id' => '16', 'name' => 'Highly reproducible', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-11-09 14:21:15', 'created' => '2015-05-11 05:24:25', 'ProductsFeature' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '37', 'name' => 'Up to 20 PreOmics' cartridges', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-10-28 14:35:19', 'created' => '2019-10-28 14:25:09', 'ProductsFeature' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'One-tube workflow', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-10-28 14:26:43', 'created' => '2019-10-28 14:26:43', 'ProductsFeature' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '40', 'name' => 'Minimize sample loss', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-10-28 14:35:50', 'created' => '2019-10-28 14:33:24', 'ProductsFeature' => array( [maximum depth reached] ) ) ), 'Image' => array( (int) 0 => array( 'id' => '1801', 'name' => 'https://www.diagenode.com/img/product/shearing_technologies/tube-holder-pico-leap.jpg', 'alt' => 'Bioruptor Pico Leap tube holder', 'modified' => '2019-10-28 11:51:18', 'created' => '2019-10-28 11:51:18', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( 'id' => '70', 'name' => 'iST Sample Preparation with the Bioruptor®', 'description' => '<p>The PreOmics iST sample preparation of proteins combined with the Diagenode Bioruptor® Pico enhances mass spectrometric analyses.</p>', 'image_id' => '247', 'type' => 'Protocol', 'url' => 'files/protocols/AN-Preomics-Bioruptor-12_2021.pdf', 'slug' => 'preomics-bioruptor-application-note', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-17 15:26:56', 'created' => '2017-07-13 10:47:59', 'ProductsProtocol' => array( [maximum depth reached] ) ) ), 'Publication' => array(), 'Testimonial' => array( (int) 0 => array( 'id' => '77', 'name' => 'From PreOmics about Bioruptor® Pico sonication device', 'description' => '<p>We at PreOmics are using the Bioruptor® Pico on a daily basis along with the iST kits for our protein sample processing workflows. The ultrasonication-based lysis greatly improves cell and tissue disruption, as well as shearing of DNA, generating samples with significantly increased peptide and protein identifications. The Bioruptor Pico in our lab has successfully processed several thousands of samples such as cells, mammalian and plant tissues, bacteria or yeast. We are highly satisfied with the handling and reproducibility of the instrument and recommend it to improve sample preparation for protein analysis.</p>', 'author' => 'Dr. Fabian Hosp, Head of Applications, PreOmics, Martinsried, Germany', 'featured' => true, 'slug' => 'preomics-pico', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-10-28 15:30:11', 'created' => '2019-10-28 15:27:20', 'ProductsTestimonial' => array( [maximum depth reached] ) ) ), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '<blockquote><span class="label-green" style="margin-bottom:16px;margin-left:-22px">TESTIMONIAL</span><p>We at PreOmics are using the Bioruptor® Pico on a daily basis along with the iST kits for our protein sample processing workflows. The ultrasonication-based lysis greatly improves cell and tissue disruption, as well as shearing of DNA, generating samples with significantly increased peptide and protein identifications. The Bioruptor Pico in our lab has successfully processed several thousands of samples such as cells, mammalian and plant tissues, bacteria or yeast. We are highly satisfied with the handling and reproducibility of the instrument and recommend it to improve sample preparation for protein analysis.</p><cite>Dr. Fabian Hosp, Head of Applications, PreOmics, Martinsried, Germany</cite></blockquote> ' $testimonial = array( 'id' => '77', 'name' => 'From PreOmics about Bioruptor® Pico sonication device', 'description' => '<p>We at PreOmics are using the Bioruptor® Pico on a daily basis along with the iST kits for our protein sample processing workflows. The ultrasonication-based lysis greatly improves cell and tissue disruption, as well as shearing of DNA, generating samples with significantly increased peptide and protein identifications. The Bioruptor Pico in our lab has successfully processed several thousands of samples such as cells, mammalian and plant tissues, bacteria or yeast. We are highly satisfied with the handling and reproducibility of the instrument and recommend it to improve sample preparation for protein analysis.</p>', 'author' => 'Dr. Fabian Hosp, Head of Applications, PreOmics, Martinsried, Germany', 'featured' => true, 'slug' => 'preomics-pico', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-10-28 15:30:11', 'created' => '2019-10-28 15:27:20', 'ProductsTestimonial' => array( 'id' => '183', 'product_id' => '3039', 'testimonial_id' => '77' ) ) $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/bioruptor-pico-sonication-device"><img src="/img/product/shearing_technologies/bioruptor_pico.jpg" alt="Bioruptor pico next gen sequencing " class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01060010</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Picoruptor Sonicator</h6> </div> </div> </li> ' $related = array( 'id' => '1787', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Pico sonication device', 'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p> <p>The Bioruptor® Pico (2013-2019) represented a breakthrough for shearing micro-volumes of 5 μl to larger volumes of up to 2 ml. <span>The new generation keeps the features you like the most and bring even more innovation. Check it now:</span></p> <center><span></span></center><center><a href="https://www.diagenode.com/p/bioruptorpico2"> <img alt="New Bioruptor Pico" src="https://www.diagenode.com/img/product/shearing_technologies/new-pico-product-banner.jpg" /></a></center> <p></p> <p><span>Watch our short video about the Bioruptor Pico and how it can help you accomplish perfect shearing for any application including chromatin shearing, DNA shearing for NGS, unmatched DNA extraction from FFPE samples, RNA shearing, protein extraction, and much more.</span></p> <p> <script>// <![CDATA[ (function(){var qs,js,q,s,d=document,gi=d.getElementById,ce=d.createElement,gt=d.getElementsByTagName,id='typef_orm',b='https://s3-eu-west-1.amazonaws.com/share.typeform.com/';if(!gi.call(d,id)){js=ce.call(d,'script');js.id=id;js.src=b+'share.js';q=gt.call(d,'script')[0];q.parentNode.insertBefore(js,q)}id=id+'_';if(!gi.call(d,id)){qs=ce.call(d,'link');qs.rel='stylesheet';qs.id=id;qs.href=b+'share-button.css';s=gt.call(d,'head')[0];s.appendChild(qs,s)}})() // ]]></script> </p> <center><iframe width="560" height="315" src="https://www.youtube.com/embed/ckLc4owudIM" frameborder="0" allowfullscreen="allowfullscreen"></iframe></center><center> <p></p> </center><center><a href="https://www.diagenode.com/en/pages/osha"><img src="https://www.diagenode.com/img/banners/banner-osha-580.jpg" width="635" height="243" /></a></center> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div 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Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table style="width: 925px;"> <tbody> <tr valign="middle"> <td style="width: 213px;"></td> <td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div 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Check it now:</span></p> <center><span></span></center><center><a href="https://www.diagenode.com/p/bioruptorpico2"> <img alt="New Bioruptor Pico" src="https://www.diagenode.com/img/product/shearing_technologies/new-pico-product-banner.jpg" /></a></center> <p></p> <p><span>Watch our short video about the Bioruptor Pico and how it can help you accomplish perfect shearing for any application including chromatin shearing, DNA shearing for NGS, unmatched DNA extraction from FFPE samples, RNA shearing, protein extraction, and much more.</span></p> <p> <script>// <![CDATA[ (function(){var 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Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table style="width: 925px;"> <tbody> <tr valign="middle"> <td style="width: 213px;"></td> <td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center; 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Our protein research products include the Bioruptor Plus sonication device for protein extraction, protein extraction beads, protein extraction kits, unique Western blot ladders that can be directly visualized on film, and highly validated antibodies for Western blot and ChIP-seq.</p> </div> </div>', 'in_footer' => true, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'protein-analysis', 'meta_keywords' => 'Protein Analysis,Protein extraction,Western blot,ChIP-sequencing,protein extraction kits', 'meta_description' => 'Diagenode offers protein research products for protein extraction beads,kits, unique Western blot ladders that can be directly visualized on film and highly validated antibodies for Western blot and ChIP-seq.', 'meta_title' => 'Unique and robust tools to both isolate and analyze proteins | Diagenode', 'modified' => '2015-08-14 14:26:48', 'created' => '2015-01-06 09:02:19', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'Protein extraction', 'description' => '<div class="row"> <div class="large-12 columns">Various biochemical and analytical techniques require the extraction of protein from tissues or mammalian, yeast and bacterial cells. Obtaining high quality and yields of proteins is important for further downstream protein characterization such as in PAGE, western blotting, mass spectrometry or protein purification. The efficient disruption and homogenization of tissues and cultured cells obtained in just one step using <strong>Diagenode's Bioruptor</strong><sup>®</sup> deliver high quality protein.</div> </div> <p></p> <div class="row"> <div class="small-6 medium-6 large-6 columns text-center"><img src="https://www.diagenode.com/img/applications/protein_extraction_standard_plus.png" /> <p><small>Western blot analysis of GAPDH and HSP90 proteins in tissues (various mouse tissues) and cultured cell extracts (HeLA).</small></p> </div> <div class="small-6 medium-6 large-6 columns text-center"><img src="https://www.diagenode.com/img/applications/protein_extraction_pico.png" /> <p><small>Western blot analysis of GAPDH and ß-tubulin proteins in tissues (mouse liver) and cultured cell extracts (HeLA).</small></p> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'protein-extraction', 'meta_keywords' => 'Protein extraction,Bioruptor,Sonication,Protein Analysis', 'meta_description' => 'Diagenode provides efficient disruption and homogenization of tissues and cultured cells obtained in just one step using Bioruptor® deliver high quality protein.', 'meta_title' => 'Protein extraction using Bioruptor® Sonication device | Diagenode', 'modified' => '2017-10-16 14:39:42', 'created' => '2014-07-02 04:41:03', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '40', 'position' => '1', 'parent_id' => '6', 'name' => 'Bioruptor<sup>®</sup> Pico accessories', 'description' => '<p>All you need for the smooth running of the Bioruptor<sup>®</sup> Pico. Download our quick guide <a href="../documents/bioruptor-organigram-tubes">"Which tubes for which Bioruptor<sup><span>®</span></sup>?"</a> to find the best tubes for your applications.</p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'bioruptor-pico-accessories', 'cookies_tag_id' => null, 'meta_keywords' => 'Bioruptor® Pico accessories,DNA shearing ,chromatin shearing,sonication device,sonicator', 'meta_description' => 'All you need for the smooth running of the Bioruptor® Pico', 'meta_title' => 'Accessories for Bioruptor® Pico sonication device | Diagenode', 'modified' => '2018-02-14 16:24:24', 'created' => '2015-06-12 10:01:53', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '1061', 'name' => 'Increase your iST ultrasonication throughput with the new Bioruptor® Pico cartridge holder', 'description' => '<p><span>The PreOmics iST sample preparation of proteins combined with the Diagenode Bioruptor® Pico enhances mass spectrometric analyses. We have previously demonstrated that the number of peptide and protein identifications correlates with the number of sonication cycles, increasing protein and peptide identifications by up to 22% and 72%. Thus, the ultrasonication-based cell lysis aids in proteome-wide discovery by greatly enhances the number of peptide and protein identifications.</span></p> <p><span><span>The <a href="https://www.diagenode.com/en/products/view/3039">new Bioruptor cartridge holder</a> allows placing of up to 20 iST cartridges directly into the <a href="https://www.diagenode.com/en/p/bioruptor-pico-sonication-device">Bioruptor Pico</a>, thus minimizing sample loss and increasing throughput.</span><br /></span></p>', 'image_id' => null, 'type' => 'White Paper', 'url' => 'files/products/shearing_technology/bioruptor_accessories/whitepaper-ist-adaptors.pdf', 'slug' => 'wp-ist-adaptators', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-10-28 16:31:40', 'created' => '2019-10-28 15:23:29', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array( (int) 0 => array( 'id' => '16', 'name' => 'Highly reproducible', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-11-09 14:21:15', 'created' => '2015-05-11 05:24:25', 'ProductsFeature' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '37', 'name' => 'Up to 20 PreOmics' cartridges', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-10-28 14:35:19', 'created' => '2019-10-28 14:25:09', 'ProductsFeature' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'One-tube workflow', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-10-28 14:26:43', 'created' => '2019-10-28 14:26:43', 'ProductsFeature' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '40', 'name' => 'Minimize sample loss', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-10-28 14:35:50', 'created' => '2019-10-28 14:33:24', 'ProductsFeature' => array( [maximum depth reached] ) ) ), 'Image' => array( (int) 0 => array( 'id' => '1801', 'name' => 'https://www.diagenode.com/img/product/shearing_technologies/tube-holder-pico-leap.jpg', 'alt' => 'Bioruptor Pico Leap tube holder', 'modified' => '2019-10-28 11:51:18', 'created' => '2019-10-28 11:51:18', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( 'id' => '70', 'name' => 'iST Sample Preparation with the Bioruptor®', 'description' => '<p>The PreOmics iST sample preparation of proteins combined with the Diagenode Bioruptor® Pico enhances mass spectrometric analyses.</p>', 'image_id' => '247', 'type' => 'Protocol', 'url' => 'files/protocols/AN-Preomics-Bioruptor-12_2021.pdf', 'slug' => 'preomics-bioruptor-application-note', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-17 15:26:56', 'created' => '2017-07-13 10:47:59', 'ProductsProtocol' => array( [maximum depth reached] ) ) ), 'Publication' => array(), 'Testimonial' => array( (int) 0 => array( 'id' => '77', 'name' => 'From PreOmics about Bioruptor® Pico sonication device', 'description' => '<p>We at PreOmics are using the Bioruptor® Pico on a daily basis along with the iST kits for our protein sample processing workflows. The ultrasonication-based lysis greatly improves cell and tissue disruption, as well as shearing of DNA, generating samples with significantly increased peptide and protein identifications. The Bioruptor Pico in our lab has successfully processed several thousands of samples such as cells, mammalian and plant tissues, bacteria or yeast. We are highly satisfied with the handling and reproducibility of the instrument and recommend it to improve sample preparation for protein analysis.</p>', 'author' => 'Dr. Fabian Hosp, Head of Applications, PreOmics, Martinsried, Germany', 'featured' => true, 'slug' => 'preomics-pico', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-10-28 15:30:11', 'created' => '2019-10-28 15:27:20', 'ProductsTestimonial' => array( [maximum depth reached] ) ) ), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '<blockquote><span class="label-green" style="margin-bottom:16px;margin-left:-22px">TESTIMONIAL</span><p>We at PreOmics are using the Bioruptor® Pico on a daily basis along with the iST kits for our protein sample processing workflows. The ultrasonication-based lysis greatly improves cell and tissue disruption, as well as shearing of DNA, generating samples with significantly increased peptide and protein identifications. The Bioruptor Pico in our lab has successfully processed several thousands of samples such as cells, mammalian and plant tissues, bacteria or yeast. We are highly satisfied with the handling and reproducibility of the instrument and recommend it to improve sample preparation for protein analysis.</p><cite>Dr. Fabian Hosp, Head of Applications, PreOmics, Martinsried, Germany</cite></blockquote> ' $testimonial = array( 'id' => '77', 'name' => 'From PreOmics about Bioruptor® Pico sonication device', 'description' => '<p>We at PreOmics are using the Bioruptor® Pico on a daily basis along with the iST kits for our protein sample processing workflows. The ultrasonication-based lysis greatly improves cell and tissue disruption, as well as shearing of DNA, generating samples with significantly increased peptide and protein identifications. The Bioruptor Pico in our lab has successfully processed several thousands of samples such as cells, mammalian and plant tissues, bacteria or yeast. We are highly satisfied with the handling and reproducibility of the instrument and recommend it to improve sample preparation for protein analysis.</p>', 'author' => 'Dr. Fabian Hosp, Head of Applications, PreOmics, Martinsried, Germany', 'featured' => true, 'slug' => 'preomics-pico', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-10-28 15:30:11', 'created' => '2019-10-28 15:27:20', 'ProductsTestimonial' => array( 'id' => '183', 'product_id' => '3039', 'testimonial_id' => '77' ) ) $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/bioruptor-pico-sonication-device"><img src="/img/product/shearing_technologies/bioruptor_pico.jpg" alt="Bioruptor pico next gen sequencing " class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01060010</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Picoruptor Sonicator</h6> </div> </div> </li> ' $related = array( 'id' => '1787', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Pico sonication device', 'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p> <p>The Bioruptor® Pico (2013-2019) represented a breakthrough for shearing micro-volumes of 5 μl to larger volumes of up to 2 ml. <span>The new generation keeps the features you like the most and bring even more innovation. Check it now:</span></p> <center><span></span></center><center><a href="https://www.diagenode.com/p/bioruptorpico2"> <img alt="New Bioruptor Pico" src="https://www.diagenode.com/img/product/shearing_technologies/new-pico-product-banner.jpg" /></a></center> <p></p> <p><span>Watch our short video about the Bioruptor Pico and how it can help you accomplish perfect shearing for any application including chromatin shearing, DNA shearing for NGS, unmatched DNA extraction from FFPE samples, RNA shearing, protein extraction, and much more.</span></p> <p> <script>// <![CDATA[ (function(){var 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Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table style="width: 925px;"> <tbody> <tr valign="middle"> <td style="width: 213px;"></td> <td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div 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We have previously demonstrated that the number of peptide and protein identifications correlates with the number of sonication cycles, increasing protein and peptide identifications by up to 22% and 72%. Thus, the ultrasonication-based cell lysis aids in proteome-wide discovery by greatly enhances the number of peptide and protein identifications.</span></p> <p><span><span>The <a href="https://www.diagenode.com/en/products/view/3039">new Bioruptor cartridge holder</a> allows placing of up to 20 iST cartridges directly into the <a href="https://www.diagenode.com/en/p/bioruptor-pico-sonication-device">Bioruptor Pico</a>, thus minimizing sample loss and increasing throughput.</span><br /></span></p>', 'image_id' => null, 'type' => 'White Paper', 'url' => 'files/products/shearing_technology/bioruptor_accessories/whitepaper-ist-adaptors.pdf', 'slug' => 'wp-ist-adaptators', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-10-28 16:31:40', 'created' => '2019-10-28 15:23:29', 'ProductsDocument' => array( 'id' => '2795', 'product_id' => '3039', 'document_id' => '1061' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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Check it now:</span></p> <center><span></span></center><center><a href="https://www.diagenode.com/p/bioruptorpico2"> <img alt="New Bioruptor Pico" src="https://www.diagenode.com/img/product/shearing_technologies/new-pico-product-banner.jpg" /></a></center> <p></p> <p><span>Watch our short video about the Bioruptor Pico and how it can help you accomplish perfect shearing for any application including chromatin shearing, DNA shearing for NGS, unmatched DNA extraction from FFPE samples, RNA shearing, protein extraction, and much more.</span></p> <p> <script>// <![CDATA[ (function(){var 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Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table style="width: 925px;"> <tbody> <tr valign="middle"> <td style="width: 213px;"></td> <td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center; 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The efficient disruption and homogenization of tissues and cultured cells obtained in just one step using <strong>Diagenode's Bioruptor</strong><sup>®</sup> deliver high quality protein.</div> </div> <p></p> <div class="row"> <div class="small-6 medium-6 large-6 columns text-center"><img src="https://www.diagenode.com/img/applications/protein_extraction_standard_plus.png" /> <p><small>Western blot analysis of GAPDH and HSP90 proteins in tissues (various mouse tissues) and cultured cell extracts (HeLA).</small></p> </div> <div class="small-6 medium-6 large-6 columns text-center"><img src="https://www.diagenode.com/img/applications/protein_extraction_pico.png" /> <p><small>Western blot analysis of GAPDH and ß-tubulin proteins in tissues (mouse liver) and cultured cell extracts (HeLA).</small></p> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'protein-extraction', 'meta_keywords' => 'Protein extraction,Bioruptor,Sonication,Protein Analysis', 'meta_description' => 'Diagenode provides efficient disruption and homogenization of tissues and cultured cells obtained in just one step using Bioruptor® deliver high quality protein.', 'meta_title' => 'Protein extraction using Bioruptor® Sonication device | Diagenode', 'modified' => '2017-10-16 14:39:42', 'created' => '2014-07-02 04:41:03', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '40', 'position' => '1', 'parent_id' => '6', 'name' => 'Bioruptor<sup>®</sup> Pico accessories', 'description' => '<p>All you need for the smooth running of the Bioruptor<sup>®</sup> Pico. Download our quick guide <a href="../documents/bioruptor-organigram-tubes">"Which tubes for which Bioruptor<sup><span>®</span></sup>?"</a> to find the best tubes for your applications.</p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'bioruptor-pico-accessories', 'cookies_tag_id' => null, 'meta_keywords' => 'Bioruptor® Pico accessories,DNA shearing ,chromatin shearing,sonication device,sonicator', 'meta_description' => 'All you need for the smooth running of the Bioruptor® Pico', 'meta_title' => 'Accessories for Bioruptor® Pico sonication device | Diagenode', 'modified' => '2018-02-14 16:24:24', 'created' => '2015-06-12 10:01:53', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '1061', 'name' => 'Increase your iST ultrasonication throughput with the new Bioruptor® Pico cartridge holder', 'description' => '<p><span>The PreOmics iST sample preparation of proteins combined with the Diagenode Bioruptor® Pico enhances mass spectrometric analyses. 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Thus, the ultrasonication-based cell lysis aids in proteome-wide discovery by greatly enhances the number of peptide and protein identifications.</span></p> <p><span><span>The <a href="https://www.diagenode.com/en/products/view/3039">new Bioruptor cartridge holder</a> allows placing of up to 20 iST cartridges directly into the <a href="https://www.diagenode.com/en/p/bioruptor-pico-sonication-device">Bioruptor Pico</a>, thus minimizing sample loss and increasing throughput.</span><br /></span></p>', 'image_id' => null, 'type' => 'White Paper', 'url' => 'files/products/shearing_technology/bioruptor_accessories/whitepaper-ist-adaptors.pdf', 'slug' => 'wp-ist-adaptators', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-10-28 16:31:40', 'created' => '2019-10-28 15:23:29', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array( (int) 0 => array( 'id' => '16', 'name' => 'Highly reproducible', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-11-09 14:21:15', 'created' => '2015-05-11 05:24:25', 'ProductsFeature' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '37', 'name' => 'Up to 20 PreOmics' cartridges', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-10-28 14:35:19', 'created' => '2019-10-28 14:25:09', 'ProductsFeature' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'One-tube workflow', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-10-28 14:26:43', 'created' => '2019-10-28 14:26:43', 'ProductsFeature' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '40', 'name' => 'Minimize sample loss', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-10-28 14:35:50', 'created' => '2019-10-28 14:33:24', 'ProductsFeature' => array( [maximum depth reached] ) ) ), 'Image' => array( (int) 0 => array( 'id' => '1801', 'name' => 'https://www.diagenode.com/img/product/shearing_technologies/tube-holder-pico-leap.jpg', 'alt' => 'Bioruptor Pico Leap tube holder', 'modified' => '2019-10-28 11:51:18', 'created' => '2019-10-28 11:51:18', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( 'id' => '70', 'name' => 'iST Sample Preparation with the Bioruptor®', 'description' => '<p>The PreOmics iST sample preparation of proteins combined with the Diagenode Bioruptor® Pico enhances mass spectrometric analyses.</p>', 'image_id' => '247', 'type' => 'Protocol', 'url' => 'files/protocols/AN-Preomics-Bioruptor-12_2021.pdf', 'slug' => 'preomics-bioruptor-application-note', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-17 15:26:56', 'created' => '2017-07-13 10:47:59', 'ProductsProtocol' => array( [maximum depth reached] ) ) ), 'Publication' => array(), 'Testimonial' => array( (int) 0 => array( 'id' => '77', 'name' => 'From PreOmics about Bioruptor® Pico sonication device', 'description' => '<p>We at PreOmics are using the Bioruptor® Pico on a daily basis along with the iST kits for our protein sample processing workflows. The ultrasonication-based lysis greatly improves cell and tissue disruption, as well as shearing of DNA, generating samples with significantly increased peptide and protein identifications. The Bioruptor Pico in our lab has successfully processed several thousands of samples such as cells, mammalian and plant tissues, bacteria or yeast. We are highly satisfied with the handling and reproducibility of the instrument and recommend it to improve sample preparation for protein analysis.</p>', 'author' => 'Dr. Fabian Hosp, Head of Applications, PreOmics, Martinsried, Germany', 'featured' => true, 'slug' => 'preomics-pico', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-10-28 15:30:11', 'created' => '2019-10-28 15:27:20', 'ProductsTestimonial' => array( [maximum depth reached] ) ) ), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '<blockquote><span class="label-green" style="margin-bottom:16px;margin-left:-22px">TESTIMONIAL</span><p>We at PreOmics are using the Bioruptor® Pico on a daily basis along with the iST kits for our protein sample processing workflows. The ultrasonication-based lysis greatly improves cell and tissue disruption, as well as shearing of DNA, generating samples with significantly increased peptide and protein identifications. The Bioruptor Pico in our lab has successfully processed several thousands of samples such as cells, mammalian and plant tissues, bacteria or yeast. We are highly satisfied with the handling and reproducibility of the instrument and recommend it to improve sample preparation for protein analysis.</p><cite>Dr. Fabian Hosp, Head of Applications, PreOmics, Martinsried, Germany</cite></blockquote> ' $testimonial = array( 'id' => '77', 'name' => 'From PreOmics about Bioruptor® Pico sonication device', 'description' => '<p>We at PreOmics are using the Bioruptor® Pico on a daily basis along with the iST kits for our protein sample processing workflows. The ultrasonication-based lysis greatly improves cell and tissue disruption, as well as shearing of DNA, generating samples with significantly increased peptide and protein identifications. The Bioruptor Pico in our lab has successfully processed several thousands of samples such as cells, mammalian and plant tissues, bacteria or yeast. We are highly satisfied with the handling and reproducibility of the instrument and recommend it to improve sample preparation for protein analysis.</p>', 'author' => 'Dr. Fabian Hosp, Head of Applications, PreOmics, Martinsried, Germany', 'featured' => true, 'slug' => 'preomics-pico', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-10-28 15:30:11', 'created' => '2019-10-28 15:27:20', 'ProductsTestimonial' => array( 'id' => '183', 'product_id' => '3039', 'testimonial_id' => '77' ) ) $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/bioruptor-pico-sonication-device"><img src="/img/product/shearing_technologies/bioruptor_pico.jpg" alt="Bioruptor pico next gen sequencing " class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01060010</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Picoruptor Sonicator</h6> </div> </div> </li> ' $related = array( 'id' => '1787', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Pico sonication device', 'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p> <p>The Bioruptor® Pico (2013-2019) represented a breakthrough for shearing micro-volumes of 5 μl to larger volumes of up to 2 ml. <span>The new generation keeps the features you like the most and bring even more innovation. Check it now:</span></p> <center><span></span></center><center><a href="https://www.diagenode.com/p/bioruptorpico2"> <img alt="New Bioruptor Pico" src="https://www.diagenode.com/img/product/shearing_technologies/new-pico-product-banner.jpg" /></a></center> <p></p> <p><span>Watch our short video about the Bioruptor Pico and how it can help you accomplish perfect shearing for any application including chromatin shearing, DNA shearing for NGS, unmatched DNA extraction from FFPE samples, RNA shearing, protein extraction, and much more.</span></p> <p> <script>// <![CDATA[ (function(){var qs,js,q,s,d=document,gi=d.getElementById,ce=d.createElement,gt=d.getElementsByTagName,id='typef_orm',b='https://s3-eu-west-1.amazonaws.com/share.typeform.com/';if(!gi.call(d,id)){js=ce.call(d,'script');js.id=id;js.src=b+'share.js';q=gt.call(d,'script')[0];q.parentNode.insertBefore(js,q)}id=id+'_';if(!gi.call(d,id)){qs=ce.call(d,'link');qs.rel='stylesheet';qs.id=id;qs.href=b+'share-button.css';s=gt.call(d,'head')[0];s.appendChild(qs,s)}})() // ]]></script> </p> <center><iframe width="560" height="315" src="https://www.youtube.com/embed/ckLc4owudIM" frameborder="0" allowfullscreen="allowfullscreen"></iframe></center><center> <p></p> </center><center><a href="https://www.diagenode.com/en/pages/osha"><img src="https://www.diagenode.com/img/banners/banner-osha-580.jpg" width="635" height="243" /></a></center> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div 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Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table style="width: 925px;"> <tbody> <tr valign="middle"> <td style="width: 213px;"></td> <td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div 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Check it now:</span></p> <center><span></span></center><center><a href="https://www.diagenode.com/p/bioruptorpico2"> <img alt="New Bioruptor Pico" src="https://www.diagenode.com/img/product/shearing_technologies/new-pico-product-banner.jpg" /></a></center> <p></p> <p><span>Watch our short video about the Bioruptor Pico and how it can help you accomplish perfect shearing for any application including chromatin shearing, DNA shearing for NGS, unmatched DNA extraction from FFPE samples, RNA shearing, protein extraction, and much more.</span></p> <p> <script>// <![CDATA[ (function(){var 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Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table style="width: 925px;"> <tbody> <tr valign="middle"> <td style="width: 213px;"></td> <td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center; 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Our protein research products include the Bioruptor Plus sonication device for protein extraction, protein extraction beads, protein extraction kits, unique Western blot ladders that can be directly visualized on film, and highly validated antibodies for Western blot and ChIP-seq.</p> </div> </div>', 'in_footer' => true, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'protein-analysis', 'meta_keywords' => 'Protein Analysis,Protein extraction,Western blot,ChIP-sequencing,protein extraction kits', 'meta_description' => 'Diagenode offers protein research products for protein extraction beads,kits, unique Western blot ladders that can be directly visualized on film and highly validated antibodies for Western blot and ChIP-seq.', 'meta_title' => 'Unique and robust tools to both isolate and analyze proteins | Diagenode', 'modified' => '2015-08-14 14:26:48', 'created' => '2015-01-06 09:02:19', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'Protein extraction', 'description' => '<div class="row"> <div class="large-12 columns">Various biochemical and analytical techniques require the extraction of protein from tissues or mammalian, yeast and bacterial cells. Obtaining high quality and yields of proteins is important for further downstream protein characterization such as in PAGE, western blotting, mass spectrometry or protein purification. The efficient disruption and homogenization of tissues and cultured cells obtained in just one step using <strong>Diagenode's Bioruptor</strong><sup>®</sup> deliver high quality protein.</div> </div> <p></p> <div class="row"> <div class="small-6 medium-6 large-6 columns text-center"><img src="https://www.diagenode.com/img/applications/protein_extraction_standard_plus.png" /> <p><small>Western blot analysis of GAPDH and HSP90 proteins in tissues (various mouse tissues) and cultured cell extracts (HeLA).</small></p> </div> <div class="small-6 medium-6 large-6 columns text-center"><img src="https://www.diagenode.com/img/applications/protein_extraction_pico.png" /> <p><small>Western blot analysis of GAPDH and ß-tubulin proteins in tissues (mouse liver) and cultured cell extracts (HeLA).</small></p> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'protein-extraction', 'meta_keywords' => 'Protein extraction,Bioruptor,Sonication,Protein Analysis', 'meta_description' => 'Diagenode provides efficient disruption and homogenization of tissues and cultured cells obtained in just one step using Bioruptor® deliver high quality protein.', 'meta_title' => 'Protein extraction using Bioruptor® Sonication device | Diagenode', 'modified' => '2017-10-16 14:39:42', 'created' => '2014-07-02 04:41:03', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '40', 'position' => '1', 'parent_id' => '6', 'name' => 'Bioruptor<sup>®</sup> Pico accessories', 'description' => '<p>All you need for the smooth running of the Bioruptor<sup>®</sup> Pico. Download our quick guide <a href="../documents/bioruptor-organigram-tubes">"Which tubes for which Bioruptor<sup><span>®</span></sup>?"</a> to find the best tubes for your applications.</p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'bioruptor-pico-accessories', 'cookies_tag_id' => null, 'meta_keywords' => 'Bioruptor® Pico accessories,DNA shearing ,chromatin shearing,sonication device,sonicator', 'meta_description' => 'All you need for the smooth running of the Bioruptor® Pico', 'meta_title' => 'Accessories for Bioruptor® Pico sonication device | Diagenode', 'modified' => '2018-02-14 16:24:24', 'created' => '2015-06-12 10:01:53', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '1061', 'name' => 'Increase your iST ultrasonication throughput with the new Bioruptor® Pico cartridge holder', 'description' => '<p><span>The PreOmics iST sample preparation of proteins combined with the Diagenode Bioruptor® Pico enhances mass spectrometric analyses. We have previously demonstrated that the number of peptide and protein identifications correlates with the number of sonication cycles, increasing protein and peptide identifications by up to 22% and 72%. Thus, the ultrasonication-based cell lysis aids in proteome-wide discovery by greatly enhances the number of peptide and protein identifications.</span></p> <p><span><span>The <a href="https://www.diagenode.com/en/products/view/3039">new Bioruptor cartridge holder</a> allows placing of up to 20 iST cartridges directly into the <a href="https://www.diagenode.com/en/p/bioruptor-pico-sonication-device">Bioruptor Pico</a>, thus minimizing sample loss and increasing throughput.</span><br /></span></p>', 'image_id' => null, 'type' => 'White Paper', 'url' => 'files/products/shearing_technology/bioruptor_accessories/whitepaper-ist-adaptors.pdf', 'slug' => 'wp-ist-adaptators', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-10-28 16:31:40', 'created' => '2019-10-28 15:23:29', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array( (int) 0 => array( 'id' => '16', 'name' => 'Highly reproducible', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-11-09 14:21:15', 'created' => '2015-05-11 05:24:25', 'ProductsFeature' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '37', 'name' => 'Up to 20 PreOmics' cartridges', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-10-28 14:35:19', 'created' => '2019-10-28 14:25:09', 'ProductsFeature' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '38', 'name' => 'One-tube workflow', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-10-28 14:26:43', 'created' => '2019-10-28 14:26:43', 'ProductsFeature' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '40', 'name' => 'Minimize sample loss', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-10-28 14:35:50', 'created' => '2019-10-28 14:33:24', 'ProductsFeature' => array( [maximum depth reached] ) ) ), 'Image' => array( (int) 0 => array( 'id' => '1801', 'name' => 'https://www.diagenode.com/img/product/shearing_technologies/tube-holder-pico-leap.jpg', 'alt' => 'Bioruptor Pico Leap tube holder', 'modified' => '2019-10-28 11:51:18', 'created' => '2019-10-28 11:51:18', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( 'id' => '70', 'name' => 'iST Sample Preparation with the Bioruptor®', 'description' => '<p>The PreOmics iST sample preparation of proteins combined with the Diagenode Bioruptor® Pico enhances mass spectrometric analyses.</p>', 'image_id' => '247', 'type' => 'Protocol', 'url' => 'files/protocols/AN-Preomics-Bioruptor-12_2021.pdf', 'slug' => 'preomics-bioruptor-application-note', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-01-17 15:26:56', 'created' => '2017-07-13 10:47:59', 'ProductsProtocol' => array( [maximum depth reached] ) ) ), 'Publication' => array(), 'Testimonial' => array( (int) 0 => array( 'id' => '77', 'name' => 'From PreOmics about Bioruptor® Pico sonication device', 'description' => '<p>We at PreOmics are using the Bioruptor® Pico on a daily basis along with the iST kits for our protein sample processing workflows. The ultrasonication-based lysis greatly improves cell and tissue disruption, as well as shearing of DNA, generating samples with significantly increased peptide and protein identifications. The Bioruptor Pico in our lab has successfully processed several thousands of samples such as cells, mammalian and plant tissues, bacteria or yeast. We are highly satisfied with the handling and reproducibility of the instrument and recommend it to improve sample preparation for protein analysis.</p>', 'author' => 'Dr. Fabian Hosp, Head of Applications, PreOmics, Martinsried, Germany', 'featured' => true, 'slug' => 'preomics-pico', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-10-28 15:30:11', 'created' => '2019-10-28 15:27:20', 'ProductsTestimonial' => array( [maximum depth reached] ) ) ), 'Area' => array(), 'SafetySheet' => array() ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '<blockquote><span class="label-green" style="margin-bottom:16px;margin-left:-22px">TESTIMONIAL</span><p>We at PreOmics are using the Bioruptor® Pico on a daily basis along with the iST kits for our protein sample processing workflows. The ultrasonication-based lysis greatly improves cell and tissue disruption, as well as shearing of DNA, generating samples with significantly increased peptide and protein identifications. The Bioruptor Pico in our lab has successfully processed several thousands of samples such as cells, mammalian and plant tissues, bacteria or yeast. We are highly satisfied with the handling and reproducibility of the instrument and recommend it to improve sample preparation for protein analysis.</p><cite>Dr. Fabian Hosp, Head of Applications, PreOmics, Martinsried, Germany</cite></blockquote> ' $testimonial = array( 'id' => '77', 'name' => 'From PreOmics about Bioruptor® Pico sonication device', 'description' => '<p>We at PreOmics are using the Bioruptor® Pico on a daily basis along with the iST kits for our protein sample processing workflows. The ultrasonication-based lysis greatly improves cell and tissue disruption, as well as shearing of DNA, generating samples with significantly increased peptide and protein identifications. The Bioruptor Pico in our lab has successfully processed several thousands of samples such as cells, mammalian and plant tissues, bacteria or yeast. We are highly satisfied with the handling and reproducibility of the instrument and recommend it to improve sample preparation for protein analysis.</p>', 'author' => 'Dr. Fabian Hosp, Head of Applications, PreOmics, Martinsried, Germany', 'featured' => true, 'slug' => 'preomics-pico', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-10-28 15:30:11', 'created' => '2019-10-28 15:27:20', 'ProductsTestimonial' => array( 'id' => '183', 'product_id' => '3039', 'testimonial_id' => '77' ) ) $related_products = '<li> <div class="row"> <div class="small-12 columns"> <a href="/jp/p/bioruptor-pico-sonication-device"><img src="/img/product/shearing_technologies/bioruptor_pico.jpg" alt="Bioruptor pico next gen sequencing " class="th"/></a> </div> <div class="small-12 columns"> <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px"> <span class="success label" style="">B01060010</span> </div> <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px"> <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a--> </div> </div> <div class="small-12 columns" > <h6 style="height:60px">Picoruptor Sonicator</h6> </div> </div> </li> ' $related = array( 'id' => '1787', 'antibody_id' => null, 'name' => 'Bioruptor<sup>®</sup> Pico sonication device', 'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p> <p>The Bioruptor® Pico (2013-2019) represented a breakthrough for shearing micro-volumes of 5 μl to larger volumes of up to 2 ml. <span>The new generation keeps the features you like the most and bring even more innovation. Check it now:</span></p> <center><span></span></center><center><a href="https://www.diagenode.com/p/bioruptorpico2"> <img alt="New Bioruptor Pico" src="https://www.diagenode.com/img/product/shearing_technologies/new-pico-product-banner.jpg" /></a></center> <p></p> <p><span>Watch our short video about the Bioruptor Pico and how it can help you accomplish perfect shearing for any application including chromatin shearing, DNA shearing for NGS, unmatched DNA extraction from FFPE samples, RNA shearing, protein extraction, and much more.</span></p> <p> <script>// <![CDATA[ (function(){var 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data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label2' => 'Recommended settings for DNA shearing with Bioruptor® Pico', 'info2' => '<p>Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor® Pico</a></p> <p></p> <p> <script>// 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id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" 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Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> <table style="width: 925px;"> <tbody> <tr valign="middle"> <td style="width: 213px;"></td> <td style="text-align: center; width: 208px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td> <td style="text-align: center; width: 180px;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center; width: 154px;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center; width: 155px;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>SDS concentration</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">< 0.1%</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">0.2%</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">1%</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">0.5%</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">Yes</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">No</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">Yes</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;">100 million cells</p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;">up to 25 g of tissue</p> </td> </tr> <tr style="background-color: #fff;" valign="middle"> <td style="width: 213px;"> <p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center; width: 208px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> <p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p> </td> <td style="text-align: center; width: 180px;"> <p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center; width: 154px;"> <p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center; width: 155px;"> <p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div> <div 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'created' => '2017-07-13 10:47:59', 'ProductsProtocol' => array( 'id' => '239', 'product_id' => '3039', 'protocol_id' => '70' ) ) $document = array( 'id' => '1061', 'name' => 'Increase your iST ultrasonication throughput with the new Bioruptor® Pico cartridge holder', 'description' => '<p><span>The PreOmics iST sample preparation of proteins combined with the Diagenode Bioruptor® Pico enhances mass spectrometric analyses. We have previously demonstrated that the number of peptide and protein identifications correlates with the number of sonication cycles, increasing protein and peptide identifications by up to 22% and 72%. Thus, the ultrasonication-based cell lysis aids in proteome-wide discovery by greatly enhances the number of peptide and protein identifications.</span></p> <p><span><span>The <a href="https://www.diagenode.com/en/products/view/3039">new Bioruptor cartridge holder</a> allows placing of up to 20 iST cartridges directly into the <a href="https://www.diagenode.com/en/p/bioruptor-pico-sonication-device">Bioruptor Pico</a>, thus minimizing sample loss and increasing throughput.</span><br /></span></p>', 'image_id' => null, 'type' => 'White Paper', 'url' => 'files/products/shearing_technology/bioruptor_accessories/whitepaper-ist-adaptors.pdf', 'slug' => 'wp-ist-adaptators', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2019-10-28 16:31:40', 'created' => '2019-10-28 15:23:29', 'ProductsDocument' => array( 'id' => '2795', 'product_id' => '3039', 'document_id' => '1061' ) )include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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