| Datasheet Universal Mastermix DMMLD2D600 DATASHEET Datasheet description | Download |
The Universal Mastermix is a Hotstart Mastermix designed to simplify the preparation of a real-time PCR assay. The Universal Mastermix contains a Hotstart Taq NA polymerase, optimised reaction buffer, 4mM (final concentration) MgCl2 and nucleotides (dNTPs). In separate tubes, passive reference (ROX), SYBR® Green, and FITC.
| Datasheet Universal Mastermix DMMLD2D600 DATASHEET Datasheet description | Download |
| Universal Mastermix SDS GB en | Download |
| Universal Mastermix SDS US en | Download |
| Universal Mastermix SDS DE de | Download |
| Universal Mastermix SDS JP ja | Download |
| Universal Mastermix SDS BE nl | Download |
| Universal Mastermix SDS BE fr | Download |
| Universal Mastermix SDS FR fr | Download |
| Universal Mastermix SDS ES es | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: Universal Mastermix 7.5ml (Diagenode Cat# DMML-D2-D600). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Molecular Characterization and Event-Specific Real-Time PCR Detection of Two Dissimilar Groups of Genetically Modified Petunia (Petunia x hybrida) Sold on the Market |
Metabolic and Innate Immune Cues Merge into a Specific Inflammatory Response via the UPR. |
Aster Proteins Facilitate Nonvesicular Plasma Membrane to ER Cholesterol Transport in Mammalian Cells. |
Development of PCR screening assays focused on genecoding sequences for GMO detection |
Development of Real-time PCR Assays for the Detection of the pin II Terminator (tpinII) Used in GM Constructs and Its Donor Organism, Potato (Solanum tuberosum) |
Detection of Hermetia illucens by real-time PCR |
Impact of Lactobacillus plantarum ST-III on the composition of infant gut microbiota and its potential synergism with breast milk and infant formula as revealed by an in vitro study |
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J., Stolz Andrea, Grohmann Lutz', 'description' => '<p>Petunia plants with unusual orange flowers were noticed on the European market and confirmed to be genetically modified (GM) by the Finnish authorities in spring 2017. Later in 2017, inspections and controls performed by several official laboratories of national competent authorities in the European Union detected several GM petunia varieties with orange flowers, but also another group of unusually colored flowers. In the latter group, a so far undetected gene coding for a flavonoid 3’5’ hydroxylase (F3’5’H) responsible for the purple color was identified by German and Dutch authorities, suggesting that the petunias found on the markets contain different genetic constructs. Here, a strategy is described for the identification of GM petunia varieties. It is based on an initial GMO screening for known elements using (real-time) PCR and subsequent identification of the insertion sites by a gene walking-like approach called ALF (amplification of linearly-enriched fragments) in combination with Sanger and MinION sequencing. The results indicate that the positively identified GM petunias can be traced back to two dissimilar GM events used for breeding of the different varieties. The test results also confirm that the transgenic petunia event RL01-17 used in the first German field trial in 1991 is not the origin of the GM petunias sold on the market. On basis of the obtained sequence data, event-specific real-time PCR confirmatory methods were developed and validated. These methods are applicable for the rapid detection and identification of GM petunias in routine analysis. In addition, a decision support system was developed for revealing the most likely origin of the GM petunia.</p>', 'date' => '2020-07-14', 'pmid' => 'https://www.frontiersin.org/articles/10.3389/fpls.2020.01047/full', 'doi' => '10.3389/fpls.2020.01047', 'modified' => '2020-09-01 15:00:25', 'created' => '2020-08-21 16:41:39', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3738', 'name' => 'Metabolic and Innate Immune Cues Merge into a Specific Inflammatory Response via the UPR.', 'authors' => 'Mogilenko DA, Haas JT, L'homme L, Fleury S, Quemener S, Levavasseur M, Becquart C, Wartelle J, Bogomolova A, Pineau L, Molendi-Coste O, Lancel S, Dehondt H, Gheeraert C, Melchior A, Dewas C, Nikitin A, Pic S, Rabhi N, Annicotte JS, Oyadomari S, Velasco-He', 'description' => '<p>Innate immune responses are intricately linked with intracellular metabolism of myeloid cells. Toll-like receptor (TLR) stimulation shifts intracellular metabolism toward glycolysis, while anti-inflammatory signals depend on enhanced mitochondrial respiration. How exogenous metabolic signals affect the immune response is unknown. We demonstrate that TLR-dependent responses of dendritic cells (DCs) are exacerbated by a high-fatty-acid (FA) metabolic environment. FAs suppress the TLR-induced hexokinase activity and perturb tricarboxylic acid cycle metabolism. These metabolic changes enhance mitochondrial reactive oxygen species (mtROS) production and, in turn, the unfolded protein response (UPR), leading to a distinct transcriptomic signature with IL-23 as hallmark. Interestingly, chemical or genetic suppression of glycolysis was sufficient to induce this specific immune response. Conversely, reducing mtROS production or DC-specific deficiency in XBP1 attenuated IL-23 expression and skin inflammation in an IL-23-dependent model of psoriasis. Thus, fine-tuning of innate immunity depends on optimization of metabolic demands and minimization of mtROS-induced UPR.</p>', 'date' => '2019-05-16', 'pmid' => 'http://www.pubmed.gov/31031005', 'doi' => '10.1016/j.cell.2019.03.018', 'modified' => '2019-08-06 16:57:45', 'created' => '2019-07-31 13:35:50', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '3422', 'name' => 'Aster Proteins Facilitate Nonvesicular Plasma Membrane to ER Cholesterol Transport in Mammalian Cells.', 'authors' => 'Sandhu J, Li S, Fairall L, Pfisterer SG, Gurnett JE, Xiao X, Weston TA, Vashi D, Ferrari A, Orozco JL, Hartman CL, Strugatsky D, Lee SD, He C, Hong C, Jiang H, Bentolila LA, Gatta AT, Levine TP, Ferng A, Lee R, Ford DA, Young SG, Ikonen E, Schwabe JWR, To', 'description' => '<p>The mechanisms underlying sterol transport in mammalian cells are poorly understood. In particular, how cholesterol internalized from HDL is made available to the cell for storage or modification is unknown. Here, we describe three ER-resident proteins (Aster-A, -B, -C) that bind cholesterol and facilitate its removal from the plasma membrane. The crystal structure of the central domain of Aster-A broadly resembles the sterol-binding fold of mammalian StARD proteins, but sequence differences in the Aster pocket result in a distinct mode of ligand binding. The Aster N-terminal GRAM domain binds phosphatidylserine and mediates Aster recruitment to plasma membrane-ER contact sites in response to cholesterol accumulation in the plasma membrane. Mice lacking Aster-B are deficient in adrenal cholesterol ester storage and steroidogenesis because of an inability to transport cholesterol from SR-BI to the ER. These findings identify a nonvesicular pathway for plasma membrane to ER sterol trafficking in mammals.</p>', 'date' => '2018-10-04', 'pmid' => 'http://www.pubmed.gov/30220461', 'doi' => '10.1016/j.cell.2018.08.033', 'modified' => '2018-12-31 11:27:24', 'created' => '2018-12-04 09:51:07', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '3506', 'name' => 'Development of PCR screening assays focused on genecoding sequences for GMO detection', 'authors' => 'Frédéric Debode, Éric Janssen, Gilbert Berben', 'description' => '<p>For the detection of genetically modifed organisms (GMO), screening is the frst step used to determine if a GMO or its derived products are present in food or feed. If the result is positive, additional tests must be done to identify and, where necessary, quantify the genetically modifed (GM) event present. The screening must be as wide as possible to cover the different possible GM events encountered on the market. In this paper, we present new real-time PCR screening methods (DNA-based methods) using hybridization probes (TaqMan probes) focused on gene-coding regions. These were selected on the basis of their occurrence in GM constructs (Block et al., 2013). Detection is also possible by searching for the new proteins using immunoassays (ELISA or immunochromatographic test strips). These techniques are well-suited for the detection of proteins produced by the EPSPS, pat, bar and cry genes (Stave, 2002; Van den Bulcke et al., 2007). However, this approach is only suitable for raw and unprocessed products. DNA-based methods were therefore preferred in this study. PCR assays focused on genes can be combined with those focused on promoters and terminators (Debode et al., 2013) in order to provide screening with wider coverage. The PCR assays developed in this paper were based on the sequences of the bar, pat, EPSPS, gox, gus and hsp70 genes.</p>', 'date' => '2018-09-19', 'pmid' => 'https://popups.uliege.be/1780-4507/index.php?id=16568', 'doi' => '10.25518/1780-4507.16568', 'modified' => '2022-05-18 18:42:38', 'created' => '2019-02-27 12:54:44', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '3461', 'name' => 'Development of Real-time PCR Assays for the Detection of the pin II Terminator (tpinII) Used in GM Constructs and Its Donor Organism, Potato (Solanum tuberosum)', 'authors' => 'Debode Frederic, Zdeňková Kamila, Janssen Eric, Tizolova Anette, du Jardin Patrick, Berben Gilbert, Demnerova Kateřina', 'description' => '<p>G-rich DNA sequences can form four-stranded G-quadruplex (G4) secondary structures and are linked to fundamental biological processes such as transcription, replication and telomere maintenance. G4s are also implicated in promoting genome instability, cancer and other diseases. Here, we describe a detailed G4 ChIP-seq method that robustly enables the determination of G4 structure formation genome-wide in chromatin. This protocol adapts traditional ChIP-seq for the detection of DNA secondary structures through the use of a G4-structure-specific single-chain antibody with refinements in chromatin immunoprecipitation followed by high-throughput sequencing. This technology does not require expression of the G4 antibody in situ, enabling broad applicability to theoretically all chromatin sources. Beginning with chromatin isolation and antibody preparation, the entire protocol can be completed in <1 week, including basic computational analysis.</p>', 'date' => '2018-02-23', 'pmid' => 'https://link.springer.com/article/10.1007/s12161-018-1203-4', 'doi' => '10.1007/s12161-018-1203-4', 'modified' => '2019-02-18 11:58:32', 'created' => '2019-02-14 15:01:22', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '3591', 'name' => 'Detection of Hermetia illucens by real-time PCR', 'authors' => 'Marien A., Debode F., Aerts C., Ancion C., Francis F., Berben G.', 'description' => '<p>Insects are rich in proteins and could be an alternative source of macronutrients to feed animals and humans. Over the past few years, numerous companies have started producing insects for feed purposes. In Europe, the processed animal proteins obtained from seven insect species have been authorised for aquaculture by Commission Regulation (EU) 2017/893 since 1 July 2017. Methods of authentication are required to check the conformity of the products. In this study, we propose a real-time PCR method for the specific detection of the black soldier fly (Hermetia illucens L.), one of the most widely used insects for feed production. The developed PCR assays amplify a 67 bp fragment based on the mitochondrial COX3 gene coding for subunit 3 of the cytochrome c oxidase. The qualitative method was tested according to several performance criteria. The specificity was tested against 51 insect species. The specificity was also checked against plant species and other animal species such as crustaceans, mammals and birds. The sensitivity, efficiency and robustness of the PCR test were successfully tested. The applicability of the test was proven through the analysis of real-life processed samples (industrial meals) of H. illucens</p>', 'date' => '2018-01-01', 'pmid' => 'https://www.wageningenacademic.com/doi/10.3920/JIFF2017.0069', 'doi' => '10.3920/JIFF2017.0069', 'modified' => '2022-05-18 16:48:45', 'created' => '2019-04-16 12:25:30', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '3620', 'name' => 'Impact of Lactobacillus plantarum ST-III on the composition of infant gut microbiota and its potential synergism with breast milk and infant formula as revealed by an in vitro study', 'authors' => 'Yan Minghui, Chen Wanyi, Li Nan, Ren Jing, Chen Chen, You Chunping, Liu Zhenmin', 'description' => '<p>Several studies have demonstrated the effect of probiotics on prevention of diseases in infants. However, the mechanisms remain not fully addressed. In the present study a modified standard ileal efflux medium (SIEM) was exploited in an in vitro analysis and the effect of Lactobacillus plantarum ST-III on the composition of infant gut microbiota was examined in I-chip analysis followed by qPCR confirmation. Also, the effect of ST-III, when administered in combination with breast milk and infant formula, was investigated. The results showed that ST-III could reduce the overall amount of Enterobacteria and Bacteroidetes bacteria, yet increase the abundance of Lactobacillus and certain Bifidobacterium species. Notably, some species/genus of bacteria of medical significance, e.g., Alcaligenaceae sutterella and Desulfovibrio bacteria were inhibited by ST-III. Moreover, co-administration of breast milk or infant formula could increase the abundance of ST-III, suggesting a synergistic effect between ST-III and infant formula and breast milk.</p>', 'date' => '2018-01-01', 'pmid' => 'https://www.sciencedirect.com/science/article/abs/pii/S0958694618301420', 'doi' => '10.1016/j.idairyj.2018.05.014', 'modified' => '2022-05-18 18:49:31', 'created' => '2019-04-16 13:01:51', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '2454', 'name' => 'Universal Mastermix SDS GB en', 'language' => 'en', 'url' => 'files/SDS/Universal_Mastermix/SDS-DMML-D2-D600-Universal_Mastermix-GB-en-1_0.pdf', 'countries' => 'GB', 'modified' => '2022-08-30 10:56:22', 'created' => '2022-08-30 10:56:22', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2456', 'name' => 'Universal Mastermix SDS US en', 'language' => 'en', 'url' => 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'modified' => '2022-08-30 10:56:22', 'created' => '2022-08-30 10:56:22', 'ProductsSafetySheet' => array( 'id' => '4172', 'product_id' => '2642', 'safety_sheet_id' => '2452' ) ) $publication = array( 'id' => '3620', 'name' => 'Impact of Lactobacillus plantarum ST-III on the composition of infant gut microbiota and its potential synergism with breast milk and infant formula as revealed by an in vitro study', 'authors' => 'Yan Minghui, Chen Wanyi, Li Nan, Ren Jing, Chen Chen, You Chunping, Liu Zhenmin', 'description' => '<p>Several studies have demonstrated the effect of probiotics on prevention of diseases in infants. However, the mechanisms remain not fully addressed. In the present study a modified standard ileal efflux medium (SIEM) was exploited in an in vitro analysis and the effect of Lactobacillus plantarum ST-III on the composition of infant gut microbiota was examined in I-chip analysis followed by qPCR confirmation. Also, the effect of ST-III, when administered in combination with breast milk and infant formula, was investigated. The results showed that ST-III could reduce the overall amount of Enterobacteria and Bacteroidetes bacteria, yet increase the abundance of Lactobacillus and certain Bifidobacterium species. Notably, some species/genus of bacteria of medical significance, e.g., Alcaligenaceae sutterella and Desulfovibrio bacteria were inhibited by ST-III. Moreover, co-administration of breast milk or infant formula could increase the abundance of ST-III, suggesting a synergistic effect between ST-III and infant formula and breast milk.</p>', 'date' => '2018-01-01', 'pmid' => 'https://www.sciencedirect.com/science/article/abs/pii/S0958694618301420', 'doi' => '10.1016/j.idairyj.2018.05.014', 'modified' => '2022-05-18 18:49:31', 'created' => '2019-04-16 13:01:51', 'ProductsPublication' => array( 'id' => '3567', 'product_id' => '2642', 'publication_id' => '3620' ) ) $externalLink = ' <a href="https://www.sciencedirect.com/science/article/abs/pii/S0958694618301420" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
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J., Stolz Andrea, Grohmann Lutz', 'description' => '<p>Petunia plants with unusual orange flowers were noticed on the European market and confirmed to be genetically modified (GM) by the Finnish authorities in spring 2017. Later in 2017, inspections and controls performed by several official laboratories of national competent authorities in the European Union detected several GM petunia varieties with orange flowers, but also another group of unusually colored flowers. In the latter group, a so far undetected gene coding for a flavonoid 3’5’ hydroxylase (F3’5’H) responsible for the purple color was identified by German and Dutch authorities, suggesting that the petunias found on the markets contain different genetic constructs. Here, a strategy is described for the identification of GM petunia varieties. It is based on an initial GMO screening for known elements using (real-time) PCR and subsequent identification of the insertion sites by a gene walking-like approach called ALF (amplification of linearly-enriched fragments) in combination with Sanger and MinION sequencing. The results indicate that the positively identified GM petunias can be traced back to two dissimilar GM events used for breeding of the different varieties. The test results also confirm that the transgenic petunia event RL01-17 used in the first German field trial in 1991 is not the origin of the GM petunias sold on the market. On basis of the obtained sequence data, event-specific real-time PCR confirmatory methods were developed and validated. These methods are applicable for the rapid detection and identification of GM petunias in routine analysis. In addition, a decision support system was developed for revealing the most likely origin of the GM petunia.</p>', 'date' => '2020-07-14', 'pmid' => 'https://www.frontiersin.org/articles/10.3389/fpls.2020.01047/full', 'doi' => '10.3389/fpls.2020.01047', 'modified' => '2020-09-01 15:00:25', 'created' => '2020-08-21 16:41:39', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3738', 'name' => 'Metabolic and Innate Immune Cues Merge into a Specific Inflammatory Response via the UPR.', 'authors' => 'Mogilenko DA, Haas JT, L'homme L, Fleury S, Quemener S, Levavasseur M, Becquart C, Wartelle J, Bogomolova A, Pineau L, Molendi-Coste O, Lancel S, Dehondt H, Gheeraert C, Melchior A, Dewas C, Nikitin A, Pic S, Rabhi N, Annicotte JS, Oyadomari S, Velasco-He', 'description' => '<p>Innate immune responses are intricately linked with intracellular metabolism of myeloid cells. Toll-like receptor (TLR) stimulation shifts intracellular metabolism toward glycolysis, while anti-inflammatory signals depend on enhanced mitochondrial respiration. How exogenous metabolic signals affect the immune response is unknown. We demonstrate that TLR-dependent responses of dendritic cells (DCs) are exacerbated by a high-fatty-acid (FA) metabolic environment. FAs suppress the TLR-induced hexokinase activity and perturb tricarboxylic acid cycle metabolism. These metabolic changes enhance mitochondrial reactive oxygen species (mtROS) production and, in turn, the unfolded protein response (UPR), leading to a distinct transcriptomic signature with IL-23 as hallmark. Interestingly, chemical or genetic suppression of glycolysis was sufficient to induce this specific immune response. Conversely, reducing mtROS production or DC-specific deficiency in XBP1 attenuated IL-23 expression and skin inflammation in an IL-23-dependent model of psoriasis. Thus, fine-tuning of innate immunity depends on optimization of metabolic demands and minimization of mtROS-induced UPR.</p>', 'date' => '2019-05-16', 'pmid' => 'http://www.pubmed.gov/31031005', 'doi' => '10.1016/j.cell.2019.03.018', 'modified' => '2019-08-06 16:57:45', 'created' => '2019-07-31 13:35:50', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '3422', 'name' => 'Aster Proteins Facilitate Nonvesicular Plasma Membrane to ER Cholesterol Transport in Mammalian Cells.', 'authors' => 'Sandhu J, Li S, Fairall L, Pfisterer SG, Gurnett JE, Xiao X, Weston TA, Vashi D, Ferrari A, Orozco JL, Hartman CL, Strugatsky D, Lee SD, He C, Hong C, Jiang H, Bentolila LA, Gatta AT, Levine TP, Ferng A, Lee R, Ford DA, Young SG, Ikonen E, Schwabe JWR, To', 'description' => '<p>The mechanisms underlying sterol transport in mammalian cells are poorly understood. In particular, how cholesterol internalized from HDL is made available to the cell for storage or modification is unknown. Here, we describe three ER-resident proteins (Aster-A, -B, -C) that bind cholesterol and facilitate its removal from the plasma membrane. The crystal structure of the central domain of Aster-A broadly resembles the sterol-binding fold of mammalian StARD proteins, but sequence differences in the Aster pocket result in a distinct mode of ligand binding. The Aster N-terminal GRAM domain binds phosphatidylserine and mediates Aster recruitment to plasma membrane-ER contact sites in response to cholesterol accumulation in the plasma membrane. Mice lacking Aster-B are deficient in adrenal cholesterol ester storage and steroidogenesis because of an inability to transport cholesterol from SR-BI to the ER. These findings identify a nonvesicular pathway for plasma membrane to ER sterol trafficking in mammals.</p>', 'date' => '2018-10-04', 'pmid' => 'http://www.pubmed.gov/30220461', 'doi' => '10.1016/j.cell.2018.08.033', 'modified' => '2018-12-31 11:27:24', 'created' => '2018-12-04 09:51:07', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '3506', 'name' => 'Development of PCR screening assays focused on genecoding sequences for GMO detection', 'authors' => 'Frédéric Debode, Éric Janssen, Gilbert Berben', 'description' => '<p>For the detection of genetically modifed organisms (GMO), screening is the frst step used to determine if a GMO or its derived products are present in food or feed. If the result is positive, additional tests must be done to identify and, where necessary, quantify the genetically modifed (GM) event present. The screening must be as wide as possible to cover the different possible GM events encountered on the market. In this paper, we present new real-time PCR screening methods (DNA-based methods) using hybridization probes (TaqMan probes) focused on gene-coding regions. These were selected on the basis of their occurrence in GM constructs (Block et al., 2013). Detection is also possible by searching for the new proteins using immunoassays (ELISA or immunochromatographic test strips). These techniques are well-suited for the detection of proteins produced by the EPSPS, pat, bar and cry genes (Stave, 2002; Van den Bulcke et al., 2007). However, this approach is only suitable for raw and unprocessed products. DNA-based methods were therefore preferred in this study. PCR assays focused on genes can be combined with those focused on promoters and terminators (Debode et al., 2013) in order to provide screening with wider coverage. The PCR assays developed in this paper were based on the sequences of the bar, pat, EPSPS, gox, gus and hsp70 genes.</p>', 'date' => '2018-09-19', 'pmid' => 'https://popups.uliege.be/1780-4507/index.php?id=16568', 'doi' => '10.25518/1780-4507.16568', 'modified' => '2022-05-18 18:42:38', 'created' => '2019-02-27 12:54:44', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '3461', 'name' => 'Development of Real-time PCR Assays for the Detection of the pin II Terminator (tpinII) Used in GM Constructs and Its Donor Organism, Potato (Solanum tuberosum)', 'authors' => 'Debode Frederic, Zdeňková Kamila, Janssen Eric, Tizolova Anette, du Jardin Patrick, Berben Gilbert, Demnerova Kateřina', 'description' => '<p>G-rich DNA sequences can form four-stranded G-quadruplex (G4) secondary structures and are linked to fundamental biological processes such as transcription, replication and telomere maintenance. G4s are also implicated in promoting genome instability, cancer and other diseases. Here, we describe a detailed G4 ChIP-seq method that robustly enables the determination of G4 structure formation genome-wide in chromatin. This protocol adapts traditional ChIP-seq for the detection of DNA secondary structures through the use of a G4-structure-specific single-chain antibody with refinements in chromatin immunoprecipitation followed by high-throughput sequencing. This technology does not require expression of the G4 antibody in situ, enabling broad applicability to theoretically all chromatin sources. Beginning with chromatin isolation and antibody preparation, the entire protocol can be completed in <1 week, including basic computational analysis.</p>', 'date' => '2018-02-23', 'pmid' => 'https://link.springer.com/article/10.1007/s12161-018-1203-4', 'doi' => '10.1007/s12161-018-1203-4', 'modified' => '2019-02-18 11:58:32', 'created' => '2019-02-14 15:01:22', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '3591', 'name' => 'Detection of Hermetia illucens by real-time PCR', 'authors' => 'Marien A., Debode F., Aerts C., Ancion C., Francis F., Berben G.', 'description' => '<p>Insects are rich in proteins and could be an alternative source of macronutrients to feed animals and humans. Over the past few years, numerous companies have started producing insects for feed purposes. In Europe, the processed animal proteins obtained from seven insect species have been authorised for aquaculture by Commission Regulation (EU) 2017/893 since 1 July 2017. Methods of authentication are required to check the conformity of the products. In this study, we propose a real-time PCR method for the specific detection of the black soldier fly (Hermetia illucens L.), one of the most widely used insects for feed production. The developed PCR assays amplify a 67 bp fragment based on the mitochondrial COX3 gene coding for subunit 3 of the cytochrome c oxidase. The qualitative method was tested according to several performance criteria. The specificity was tested against 51 insect species. The specificity was also checked against plant species and other animal species such as crustaceans, mammals and birds. The sensitivity, efficiency and robustness of the PCR test were successfully tested. The applicability of the test was proven through the analysis of real-life processed samples (industrial meals) of H. illucens</p>', 'date' => '2018-01-01', 'pmid' => 'https://www.wageningenacademic.com/doi/10.3920/JIFF2017.0069', 'doi' => '10.3920/JIFF2017.0069', 'modified' => '2022-05-18 16:48:45', 'created' => '2019-04-16 12:25:30', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '3620', 'name' => 'Impact of Lactobacillus plantarum ST-III on the composition of infant gut microbiota and its potential synergism with breast milk and infant formula as revealed by an in vitro study', 'authors' => 'Yan Minghui, Chen Wanyi, Li Nan, Ren Jing, Chen Chen, You Chunping, Liu Zhenmin', 'description' => '<p>Several studies have demonstrated the effect of probiotics on prevention of diseases in infants. However, the mechanisms remain not fully addressed. In the present study a modified standard ileal efflux medium (SIEM) was exploited in an in vitro analysis and the effect of Lactobacillus plantarum ST-III on the composition of infant gut microbiota was examined in I-chip analysis followed by qPCR confirmation. Also, the effect of ST-III, when administered in combination with breast milk and infant formula, was investigated. The results showed that ST-III could reduce the overall amount of Enterobacteria and Bacteroidetes bacteria, yet increase the abundance of Lactobacillus and certain Bifidobacterium species. Notably, some species/genus of bacteria of medical significance, e.g., Alcaligenaceae sutterella and Desulfovibrio bacteria were inhibited by ST-III. Moreover, co-administration of breast milk or infant formula could increase the abundance of ST-III, suggesting a synergistic effect between ST-III and infant formula and breast milk.</p>', 'date' => '2018-01-01', 'pmid' => 'https://www.sciencedirect.com/science/article/abs/pii/S0958694618301420', 'doi' => '10.1016/j.idairyj.2018.05.014', 'modified' => '2022-05-18 18:49:31', 'created' => '2019-04-16 13:01:51', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '2454', 'name' => 'Universal Mastermix SDS GB en', 'language' => 'en', 'url' => 'files/SDS/Universal_Mastermix/SDS-DMML-D2-D600-Universal_Mastermix-GB-en-1_0.pdf', 'countries' => 'GB', 'modified' => '2022-08-30 10:56:22', 'created' => '2022-08-30 10:56:22', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2456', 'name' => 'Universal Mastermix SDS US en', 'language' => 'en', 'url' => 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nl', 'language' => 'nl', 'url' => 'files/SDS/Universal_Mastermix/SDS-DMML-D2-D600-Universal_Mastermix-BE-nl-1_0.pdf', 'countries' => 'BE', 'modified' => '2022-08-30 10:56:22', 'created' => '2022-08-30 10:56:22', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '2449', 'name' => 'Universal Mastermix SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/Universal_Mastermix/SDS-DMML-D2-D600-Universal_Mastermix-BE-fr-1_0.pdf', 'countries' => 'BE', 'modified' => '2022-08-30 10:56:22', 'created' => '2022-08-30 10:56:22', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '2453', 'name' => 'Universal Mastermix SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/Universal_Mastermix/SDS-DMML-D2-D600-Universal_Mastermix-FR-fr-1_0.pdf', 'countries' => 'FR', 'modified' => '2022-08-30 10:56:22', 'created' => '2022-08-30 10:56:22', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '2452', 'name' => 'Universal Mastermix SDS ES es', 'language' => 'es', 'url' => 'files/SDS/Universal_Mastermix/SDS-DMML-D2-D600-Universal_Mastermix-ES-es-1_0.pdf', 'countries' => 'ES', 'modified' => '2022-08-30 10:56:22', 'created' => '2022-08-30 10:56:22', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '<br/><small><span style="color:#CCC">(GMO-UN-A600)</span></small>' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '127', 'name' => 'Datasheet Universal Mastermix DMMLD2D600', 'description' => '<p>Datasheet description</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/Datasheet_Universal_Mastermix_DMMLD2D600.pdf', 'slug' => 'datasheet-universal-mastermix-DMMLD2D600', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-01-26 16:36:13', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( 'id' => '989', 'product_id' => '2642', 'document_id' => '127' ) ) $sds = array( 'id' => '2452', 'name' => 'Universal Mastermix SDS ES es', 'language' => 'es', 'url' => 'files/SDS/Universal_Mastermix/SDS-DMML-D2-D600-Universal_Mastermix-ES-es-1_0.pdf', 'countries' => 'ES', 'modified' => '2022-08-30 10:56:22', 'created' => '2022-08-30 10:56:22', 'ProductsSafetySheet' => array( 'id' => '4172', 'product_id' => '2642', 'safety_sheet_id' => '2452' ) ) $publication = array( 'id' => '3620', 'name' => 'Impact of Lactobacillus plantarum ST-III on the composition of infant gut microbiota and its potential synergism with breast milk and infant formula as revealed by an in vitro study', 'authors' => 'Yan Minghui, Chen Wanyi, Li Nan, Ren Jing, Chen Chen, You Chunping, Liu Zhenmin', 'description' => '<p>Several studies have demonstrated the effect of probiotics on prevention of diseases in infants. However, the mechanisms remain not fully addressed. In the present study a modified standard ileal efflux medium (SIEM) was exploited in an in vitro analysis and the effect of Lactobacillus plantarum ST-III on the composition of infant gut microbiota was examined in I-chip analysis followed by qPCR confirmation. Also, the effect of ST-III, when administered in combination with breast milk and infant formula, was investigated. The results showed that ST-III could reduce the overall amount of Enterobacteria and Bacteroidetes bacteria, yet increase the abundance of Lactobacillus and certain Bifidobacterium species. Notably, some species/genus of bacteria of medical significance, e.g., Alcaligenaceae sutterella and Desulfovibrio bacteria were inhibited by ST-III. Moreover, co-administration of breast milk or infant formula could increase the abundance of ST-III, suggesting a synergistic effect between ST-III and infant formula and breast milk.</p>', 'date' => '2018-01-01', 'pmid' => 'https://www.sciencedirect.com/science/article/abs/pii/S0958694618301420', 'doi' => '10.1016/j.idairyj.2018.05.014', 'modified' => '2022-05-18 18:49:31', 'created' => '2019-04-16 13:01:51', 'ProductsPublication' => array( 'id' => '3567', 'product_id' => '2642', 'publication_id' => '3620' ) ) $externalLink = ' <a href="https://www.sciencedirect.com/science/article/abs/pii/S0958694618301420" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
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In separate tubes, passive reference (ROX), SYBR® Green, and FITC.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '600 rxns', 'catalog_number' => 'DMML-D2-D600', 'old_catalog_number' => 'GMO-UN-A600', 'sf_code' => '', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '404', 'price_USD' => '440', 'price_GBP' => '340', 'price_JPY' => '42800', 'price_CNY' => '', 'price_AUD' => '1100', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'universal-mastermix-7-5ml-600-rxns', 'meta_title' => 'Universal Mastermix 7.5ml', 'meta_keywords' => '', 'meta_description' => 'Universal Mastermix 7.5ml', 'modified' => '2023-05-04 15:38:47', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ) ) $language = 'jp' $meta_keywords = '' $meta_description = 'Universal Mastermix 7.5ml' $meta_title = 'Universal Mastermix 7.5ml' $product = array( 'Product' => array( 'id' => '2642', 'antibody_id' => null, 'name' => 'Universal Mastermix 7.5ml', 'description' => '<p><span>The Universal Mastermix is a Hotstart Mastermix designed to simplify the preparation of a real-time PCR assay. The Universal Mastermix contains a Hotstart Taq NA polymerase, optimised reaction buffer, 4mM (final concentration) MgCl2 and nucleotides (dNTPs). In separate tubes, passive reference (ROX), SYBR® Green, and FITC.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '600 rxns', 'catalog_number' => 'DMML-D2-D600', 'old_catalog_number' => 'GMO-UN-A600', 'sf_code' => '', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '404', 'price_USD' => '440', 'price_GBP' => '340', 'price_JPY' => '42800', 'price_CNY' => '', 'price_AUD' => '1100', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'universal-mastermix-7-5ml-600-rxns', 'meta_title' => 'Universal Mastermix 7.5ml', 'meta_keywords' => '', 'meta_description' => 'Universal Mastermix 7.5ml', 'modified' => '2023-05-04 15:38:47', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '50', 'position' => '10', 'parent_id' => null, 'name' => 'Real time PCR master mix', 'description' => '', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'real-time-pcr-master-mix', 'cookies_tag_id' => null, 'meta_keywords' => 'Real time PCR master mix, Universal Mastermix,RT-PCR Mastermix', 'meta_description' => 'Diagenode offers Universal Mastermix and One-Step RT-PCR Mastermix', 'meta_title' => 'Real time PCR master mix | Diagenode', 'modified' => '2016-03-01 14:47:24', 'created' => '2015-07-07 17:00:19', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '87', 'position' => '1', 'parent_id' => '86', 'name' => 'PCR enzymes and Mixes', 'description' => '', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'PCR-enzymes-and-mixes', 'cookies_tag_id' => null, 'meta_keywords' => 'PCR enzymes and RT-PCR Mastermix ', 'meta_description' => 'Diagenode offers DNA polymerase and RT-PCR Mastermix ', 'meta_title' => 'PCR enzymes and RT-PCR Mastermix | Diagenode ', 'modified' => '2016-01-21 15:01:47', 'created' => '2015-09-16 23:10:49', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '127', 'name' => 'Datasheet Universal Mastermix DMMLD2D600', 'description' => '<p>Datasheet description</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/Datasheet_Universal_Mastermix_DMMLD2D600.pdf', 'slug' => 'datasheet-universal-mastermix-DMMLD2D600', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-01-26 16:36:13', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '3993', 'name' => 'Molecular Characterization and Event-Specific Real-Time PCR Detection of Two Dissimilar Groups of Genetically Modified Petunia (Petunia x hybrida) Sold on the Market', 'authors' => 'Voorhuijzen Marleen M., Prins Theo W., Belter Anke, Bendiek Joachim, Brünen-Nieweler Claudia, van Dijk Jeroen P., Goerlich Ottmar, Kok Esther J., Pickel Benjamin, Scholtens Ingrid M. J., Stolz Andrea, Grohmann Lutz', 'description' => '<p>Petunia plants with unusual orange flowers were noticed on the European market and confirmed to be genetically modified (GM) by the Finnish authorities in spring 2017. Later in 2017, inspections and controls performed by several official laboratories of national competent authorities in the European Union detected several GM petunia varieties with orange flowers, but also another group of unusually colored flowers. In the latter group, a so far undetected gene coding for a flavonoid 3’5’ hydroxylase (F3’5’H) responsible for the purple color was identified by German and Dutch authorities, suggesting that the petunias found on the markets contain different genetic constructs. Here, a strategy is described for the identification of GM petunia varieties. It is based on an initial GMO screening for known elements using (real-time) PCR and subsequent identification of the insertion sites by a gene walking-like approach called ALF (amplification of linearly-enriched fragments) in combination with Sanger and MinION sequencing. The results indicate that the positively identified GM petunias can be traced back to two dissimilar GM events used for breeding of the different varieties. The test results also confirm that the transgenic petunia event RL01-17 used in the first German field trial in 1991 is not the origin of the GM petunias sold on the market. On basis of the obtained sequence data, event-specific real-time PCR confirmatory methods were developed and validated. These methods are applicable for the rapid detection and identification of GM petunias in routine analysis. In addition, a decision support system was developed for revealing the most likely origin of the GM petunia.</p>', 'date' => '2020-07-14', 'pmid' => 'https://www.frontiersin.org/articles/10.3389/fpls.2020.01047/full', 'doi' => '10.3389/fpls.2020.01047', 'modified' => '2020-09-01 15:00:25', 'created' => '2020-08-21 16:41:39', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3738', 'name' => 'Metabolic and Innate Immune Cues Merge into a Specific Inflammatory Response via the UPR.', 'authors' => 'Mogilenko DA, Haas JT, L'homme L, Fleury S, Quemener S, Levavasseur M, Becquart C, Wartelle J, Bogomolova A, Pineau L, Molendi-Coste O, Lancel S, Dehondt H, Gheeraert C, Melchior A, Dewas C, Nikitin A, Pic S, Rabhi N, Annicotte JS, Oyadomari S, Velasco-He', 'description' => '<p>Innate immune responses are intricately linked with intracellular metabolism of myeloid cells. Toll-like receptor (TLR) stimulation shifts intracellular metabolism toward glycolysis, while anti-inflammatory signals depend on enhanced mitochondrial respiration. How exogenous metabolic signals affect the immune response is unknown. We demonstrate that TLR-dependent responses of dendritic cells (DCs) are exacerbated by a high-fatty-acid (FA) metabolic environment. FAs suppress the TLR-induced hexokinase activity and perturb tricarboxylic acid cycle metabolism. These metabolic changes enhance mitochondrial reactive oxygen species (mtROS) production and, in turn, the unfolded protein response (UPR), leading to a distinct transcriptomic signature with IL-23 as hallmark. Interestingly, chemical or genetic suppression of glycolysis was sufficient to induce this specific immune response. Conversely, reducing mtROS production or DC-specific deficiency in XBP1 attenuated IL-23 expression and skin inflammation in an IL-23-dependent model of psoriasis. Thus, fine-tuning of innate immunity depends on optimization of metabolic demands and minimization of mtROS-induced UPR.</p>', 'date' => '2019-05-16', 'pmid' => 'http://www.pubmed.gov/31031005', 'doi' => '10.1016/j.cell.2019.03.018', 'modified' => '2019-08-06 16:57:45', 'created' => '2019-07-31 13:35:50', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '3422', 'name' => 'Aster Proteins Facilitate Nonvesicular Plasma Membrane to ER Cholesterol Transport in Mammalian Cells.', 'authors' => 'Sandhu J, Li S, Fairall L, Pfisterer SG, Gurnett JE, Xiao X, Weston TA, Vashi D, Ferrari A, Orozco JL, Hartman CL, Strugatsky D, Lee SD, He C, Hong C, Jiang H, Bentolila LA, Gatta AT, Levine TP, Ferng A, Lee R, Ford DA, Young SG, Ikonen E, Schwabe JWR, To', 'description' => '<p>The mechanisms underlying sterol transport in mammalian cells are poorly understood. In particular, how cholesterol internalized from HDL is made available to the cell for storage or modification is unknown. Here, we describe three ER-resident proteins (Aster-A, -B, -C) that bind cholesterol and facilitate its removal from the plasma membrane. The crystal structure of the central domain of Aster-A broadly resembles the sterol-binding fold of mammalian StARD proteins, but sequence differences in the Aster pocket result in a distinct mode of ligand binding. The Aster N-terminal GRAM domain binds phosphatidylserine and mediates Aster recruitment to plasma membrane-ER contact sites in response to cholesterol accumulation in the plasma membrane. Mice lacking Aster-B are deficient in adrenal cholesterol ester storage and steroidogenesis because of an inability to transport cholesterol from SR-BI to the ER. These findings identify a nonvesicular pathway for plasma membrane to ER sterol trafficking in mammals.</p>', 'date' => '2018-10-04', 'pmid' => 'http://www.pubmed.gov/30220461', 'doi' => '10.1016/j.cell.2018.08.033', 'modified' => '2018-12-31 11:27:24', 'created' => '2018-12-04 09:51:07', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '3506', 'name' => 'Development of PCR screening assays focused on genecoding sequences for GMO detection', 'authors' => 'Frédéric Debode, Éric Janssen, Gilbert Berben', 'description' => '<p>For the detection of genetically modifed organisms (GMO), screening is the frst step used to determine if a GMO or its derived products are present in food or feed. If the result is positive, additional tests must be done to identify and, where necessary, quantify the genetically modifed (GM) event present. The screening must be as wide as possible to cover the different possible GM events encountered on the market. In this paper, we present new real-time PCR screening methods (DNA-based methods) using hybridization probes (TaqMan probes) focused on gene-coding regions. These were selected on the basis of their occurrence in GM constructs (Block et al., 2013). Detection is also possible by searching for the new proteins using immunoassays (ELISA or immunochromatographic test strips). These techniques are well-suited for the detection of proteins produced by the EPSPS, pat, bar and cry genes (Stave, 2002; Van den Bulcke et al., 2007). However, this approach is only suitable for raw and unprocessed products. DNA-based methods were therefore preferred in this study. PCR assays focused on genes can be combined with those focused on promoters and terminators (Debode et al., 2013) in order to provide screening with wider coverage. The PCR assays developed in this paper were based on the sequences of the bar, pat, EPSPS, gox, gus and hsp70 genes.</p>', 'date' => '2018-09-19', 'pmid' => 'https://popups.uliege.be/1780-4507/index.php?id=16568', 'doi' => '10.25518/1780-4507.16568', 'modified' => '2022-05-18 18:42:38', 'created' => '2019-02-27 12:54:44', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '3461', 'name' => 'Development of Real-time PCR Assays for the Detection of the pin II Terminator (tpinII) Used in GM Constructs and Its Donor Organism, Potato (Solanum tuberosum)', 'authors' => 'Debode Frederic, Zdeňková Kamila, Janssen Eric, Tizolova Anette, du Jardin Patrick, Berben Gilbert, Demnerova Kateřina', 'description' => '<p>G-rich DNA sequences can form four-stranded G-quadruplex (G4) secondary structures and are linked to fundamental biological processes such as transcription, replication and telomere maintenance. G4s are also implicated in promoting genome instability, cancer and other diseases. Here, we describe a detailed G4 ChIP-seq method that robustly enables the determination of G4 structure formation genome-wide in chromatin. This protocol adapts traditional ChIP-seq for the detection of DNA secondary structures through the use of a G4-structure-specific single-chain antibody with refinements in chromatin immunoprecipitation followed by high-throughput sequencing. This technology does not require expression of the G4 antibody in situ, enabling broad applicability to theoretically all chromatin sources. Beginning with chromatin isolation and antibody preparation, the entire protocol can be completed in <1 week, including basic computational analysis.</p>', 'date' => '2018-02-23', 'pmid' => 'https://link.springer.com/article/10.1007/s12161-018-1203-4', 'doi' => '10.1007/s12161-018-1203-4', 'modified' => '2019-02-18 11:58:32', 'created' => '2019-02-14 15:01:22', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '3591', 'name' => 'Detection of Hermetia illucens by real-time PCR', 'authors' => 'Marien A., Debode F., Aerts C., Ancion C., Francis F., Berben G.', 'description' => '<p>Insects are rich in proteins and could be an alternative source of macronutrients to feed animals and humans. Over the past few years, numerous companies have started producing insects for feed purposes. In Europe, the processed animal proteins obtained from seven insect species have been authorised for aquaculture by Commission Regulation (EU) 2017/893 since 1 July 2017. Methods of authentication are required to check the conformity of the products. In this study, we propose a real-time PCR method for the specific detection of the black soldier fly (Hermetia illucens L.), one of the most widely used insects for feed production. The developed PCR assays amplify a 67 bp fragment based on the mitochondrial COX3 gene coding for subunit 3 of the cytochrome c oxidase. The qualitative method was tested according to several performance criteria. The specificity was tested against 51 insect species. The specificity was also checked against plant species and other animal species such as crustaceans, mammals and birds. The sensitivity, efficiency and robustness of the PCR test were successfully tested. The applicability of the test was proven through the analysis of real-life processed samples (industrial meals) of H. illucens</p>', 'date' => '2018-01-01', 'pmid' => 'https://www.wageningenacademic.com/doi/10.3920/JIFF2017.0069', 'doi' => '10.3920/JIFF2017.0069', 'modified' => '2022-05-18 16:48:45', 'created' => '2019-04-16 12:25:30', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '3620', 'name' => 'Impact of Lactobacillus plantarum ST-III on the composition of infant gut microbiota and its potential synergism with breast milk and infant formula as revealed by an in vitro study', 'authors' => 'Yan Minghui, Chen Wanyi, Li Nan, Ren Jing, Chen Chen, You Chunping, Liu Zhenmin', 'description' => '<p>Several studies have demonstrated the effect of probiotics on prevention of diseases in infants. However, the mechanisms remain not fully addressed. In the present study a modified standard ileal efflux medium (SIEM) was exploited in an in vitro analysis and the effect of Lactobacillus plantarum ST-III on the composition of infant gut microbiota was examined in I-chip analysis followed by qPCR confirmation. Also, the effect of ST-III, when administered in combination with breast milk and infant formula, was investigated. The results showed that ST-III could reduce the overall amount of Enterobacteria and Bacteroidetes bacteria, yet increase the abundance of Lactobacillus and certain Bifidobacterium species. Notably, some species/genus of bacteria of medical significance, e.g., Alcaligenaceae sutterella and Desulfovibrio bacteria were inhibited by ST-III. Moreover, co-administration of breast milk or infant formula could increase the abundance of ST-III, suggesting a synergistic effect between ST-III and infant formula and breast milk.</p>', 'date' => '2018-01-01', 'pmid' => 'https://www.sciencedirect.com/science/article/abs/pii/S0958694618301420', 'doi' => '10.1016/j.idairyj.2018.05.014', 'modified' => '2022-05-18 18:49:31', 'created' => '2019-04-16 13:01:51', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '2454', 'name' => 'Universal Mastermix SDS GB en', 'language' => 'en', 'url' => 'files/SDS/Universal_Mastermix/SDS-DMML-D2-D600-Universal_Mastermix-GB-en-1_0.pdf', 'countries' => 'GB', 'modified' => '2022-08-30 10:56:22', 'created' => '2022-08-30 10:56:22', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2456', 'name' => 'Universal Mastermix SDS US en', 'language' => 'en', 'url' => 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nl', 'language' => 'nl', 'url' => 'files/SDS/Universal_Mastermix/SDS-DMML-D2-D600-Universal_Mastermix-BE-nl-1_0.pdf', 'countries' => 'BE', 'modified' => '2022-08-30 10:56:22', 'created' => '2022-08-30 10:56:22', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '2449', 'name' => 'Universal Mastermix SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/Universal_Mastermix/SDS-DMML-D2-D600-Universal_Mastermix-BE-fr-1_0.pdf', 'countries' => 'BE', 'modified' => '2022-08-30 10:56:22', 'created' => '2022-08-30 10:56:22', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '2453', 'name' => 'Universal Mastermix SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/Universal_Mastermix/SDS-DMML-D2-D600-Universal_Mastermix-FR-fr-1_0.pdf', 'countries' => 'FR', 'modified' => '2022-08-30 10:56:22', 'created' => '2022-08-30 10:56:22', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '2452', 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(int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '<br/><small><span style="color:#CCC">(GMO-UN-A600)</span></small>' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '127', 'name' => 'Datasheet Universal Mastermix DMMLD2D600', 'description' => '<p>Datasheet description</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/Datasheet_Universal_Mastermix_DMMLD2D600.pdf', 'slug' => 'datasheet-universal-mastermix-DMMLD2D600', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-01-26 16:36:13', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( 'id' => '989', 'product_id' => '2642', 'document_id' => '127' ) ) $sds = array( 'id' => '2452', 'name' => 'Universal Mastermix SDS ES es', 'language' => 'es', 'url' => 'files/SDS/Universal_Mastermix/SDS-DMML-D2-D600-Universal_Mastermix-ES-es-1_0.pdf', 'countries' => 'ES', 'modified' => '2022-08-30 10:56:22', 'created' => '2022-08-30 10:56:22', 'ProductsSafetySheet' => array( 'id' => '4172', 'product_id' => '2642', 'safety_sheet_id' => '2452' ) ) $publication = array( 'id' => '3620', 'name' => 'Impact of Lactobacillus plantarum ST-III on the composition of infant gut microbiota and its potential synergism with breast milk and infant formula as revealed by an in vitro study', 'authors' => 'Yan Minghui, Chen Wanyi, Li Nan, Ren Jing, Chen Chen, You Chunping, Liu Zhenmin', 'description' => '<p>Several studies have demonstrated the effect of probiotics on prevention of diseases in infants. However, the mechanisms remain not fully addressed. In the present study a modified standard ileal efflux medium (SIEM) was exploited in an in vitro analysis and the effect of Lactobacillus plantarum ST-III on the composition of infant gut microbiota was examined in I-chip analysis followed by qPCR confirmation. Also, the effect of ST-III, when administered in combination with breast milk and infant formula, was investigated. The results showed that ST-III could reduce the overall amount of Enterobacteria and Bacteroidetes bacteria, yet increase the abundance of Lactobacillus and certain Bifidobacterium species. Notably, some species/genus of bacteria of medical significance, e.g., Alcaligenaceae sutterella and Desulfovibrio bacteria were inhibited by ST-III. Moreover, co-administration of breast milk or infant formula could increase the abundance of ST-III, suggesting a synergistic effect between ST-III and infant formula and breast milk.</p>', 'date' => '2018-01-01', 'pmid' => 'https://www.sciencedirect.com/science/article/abs/pii/S0958694618301420', 'doi' => '10.1016/j.idairyj.2018.05.014', 'modified' => '2022-05-18 18:49:31', 'created' => '2019-04-16 13:01:51', 'ProductsPublication' => array( 'id' => '3567', 'product_id' => '2642', 'publication_id' => '3620' ) ) $externalLink = ' <a href="https://www.sciencedirect.com/science/article/abs/pii/S0958694618301420" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
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The Universal Mastermix contains a Hotstart Taq NA polymerase, optimised reaction buffer, 4mM (final concentration) MgCl2 and nucleotides (dNTPs). In separate tubes, passive reference (ROX), SYBR® Green, and FITC.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '600 rxns', 'catalog_number' => 'DMML-D2-D600', 'old_catalog_number' => 'GMO-UN-A600', 'sf_code' => '', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '404', 'price_USD' => '440', 'price_GBP' => '340', 'price_JPY' => '42800', 'price_CNY' => '', 'price_AUD' => '1100', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'universal-mastermix-7-5ml-600-rxns', 'meta_title' => 'Universal Mastermix 7.5ml', 'meta_keywords' => '', 'meta_description' => 'Universal Mastermix 7.5ml', 'modified' => '2023-05-04 15:38:47', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ) ) $language = 'jp' $meta_keywords = '' $meta_description = 'Universal Mastermix 7.5ml' $meta_title = 'Universal Mastermix 7.5ml' $product = array( 'Product' => array( 'id' => '2642', 'antibody_id' => null, 'name' => 'Universal Mastermix 7.5ml', 'description' => '<p><span>The Universal Mastermix is a Hotstart Mastermix designed to simplify the preparation of a real-time PCR assay. The Universal Mastermix contains a Hotstart Taq NA polymerase, optimised reaction buffer, 4mM (final concentration) MgCl2 and nucleotides (dNTPs). In separate tubes, passive reference (ROX), SYBR® Green, and FITC.</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '600 rxns', 'catalog_number' => 'DMML-D2-D600', 'old_catalog_number' => 'GMO-UN-A600', 'sf_code' => '', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '404', 'price_USD' => '440', 'price_GBP' => '340', 'price_JPY' => '42800', 'price_CNY' => '', 'price_AUD' => '1100', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => true, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'universal-mastermix-7-5ml-600-rxns', 'meta_title' => 'Universal Mastermix 7.5ml', 'meta_keywords' => '', 'meta_description' => 'Universal Mastermix 7.5ml', 'modified' => '2023-05-04 15:38:47', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '50', 'position' => '10', 'parent_id' => null, 'name' => 'Real time PCR master mix', 'description' => '', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'real-time-pcr-master-mix', 'cookies_tag_id' => null, 'meta_keywords' => 'Real time PCR master mix, Universal Mastermix,RT-PCR Mastermix', 'meta_description' => 'Diagenode offers Universal Mastermix and One-Step RT-PCR Mastermix', 'meta_title' => 'Real time PCR master mix | Diagenode', 'modified' => '2016-03-01 14:47:24', 'created' => '2015-07-07 17:00:19', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ), (int) 1 => array( 'id' => '87', 'position' => '1', 'parent_id' => '86', 'name' => 'PCR enzymes and Mixes', 'description' => '', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'PCR-enzymes-and-mixes', 'cookies_tag_id' => null, 'meta_keywords' => 'PCR enzymes and RT-PCR Mastermix ', 'meta_description' => 'Diagenode offers DNA polymerase and RT-PCR Mastermix ', 'meta_title' => 'PCR enzymes and RT-PCR Mastermix | Diagenode ', 'modified' => '2016-01-21 15:01:47', 'created' => '2015-09-16 23:10:49', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '127', 'name' => 'Datasheet Universal Mastermix DMMLD2D600', 'description' => '<p>Datasheet description</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/Datasheet_Universal_Mastermix_DMMLD2D600.pdf', 'slug' => 'datasheet-universal-mastermix-DMMLD2D600', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-01-26 16:36:13', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '3993', 'name' => 'Molecular Characterization and Event-Specific Real-Time PCR Detection of Two Dissimilar Groups of Genetically Modified Petunia (Petunia x hybrida) Sold on the Market', 'authors' => 'Voorhuijzen Marleen M., Prins Theo W., Belter Anke, Bendiek Joachim, Brünen-Nieweler Claudia, van Dijk Jeroen P., Goerlich Ottmar, Kok Esther J., Pickel Benjamin, Scholtens Ingrid M. J., Stolz Andrea, Grohmann Lutz', 'description' => '<p>Petunia plants with unusual orange flowers were noticed on the European market and confirmed to be genetically modified (GM) by the Finnish authorities in spring 2017. Later in 2017, inspections and controls performed by several official laboratories of national competent authorities in the European Union detected several GM petunia varieties with orange flowers, but also another group of unusually colored flowers. In the latter group, a so far undetected gene coding for a flavonoid 3’5’ hydroxylase (F3’5’H) responsible for the purple color was identified by German and Dutch authorities, suggesting that the petunias found on the markets contain different genetic constructs. Here, a strategy is described for the identification of GM petunia varieties. It is based on an initial GMO screening for known elements using (real-time) PCR and subsequent identification of the insertion sites by a gene walking-like approach called ALF (amplification of linearly-enriched fragments) in combination with Sanger and MinION sequencing. The results indicate that the positively identified GM petunias can be traced back to two dissimilar GM events used for breeding of the different varieties. The test results also confirm that the transgenic petunia event RL01-17 used in the first German field trial in 1991 is not the origin of the GM petunias sold on the market. On basis of the obtained sequence data, event-specific real-time PCR confirmatory methods were developed and validated. These methods are applicable for the rapid detection and identification of GM petunias in routine analysis. In addition, a decision support system was developed for revealing the most likely origin of the GM petunia.</p>', 'date' => '2020-07-14', 'pmid' => 'https://www.frontiersin.org/articles/10.3389/fpls.2020.01047/full', 'doi' => '10.3389/fpls.2020.01047', 'modified' => '2020-09-01 15:00:25', 'created' => '2020-08-21 16:41:39', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3738', 'name' => 'Metabolic and Innate Immune Cues Merge into a Specific Inflammatory Response via the UPR.', 'authors' => 'Mogilenko DA, Haas JT, L'homme L, Fleury S, Quemener S, Levavasseur M, Becquart C, Wartelle J, Bogomolova A, Pineau L, Molendi-Coste O, Lancel S, Dehondt H, Gheeraert C, Melchior A, Dewas C, Nikitin A, Pic S, Rabhi N, Annicotte JS, Oyadomari S, Velasco-He', 'description' => '<p>Innate immune responses are intricately linked with intracellular metabolism of myeloid cells. Toll-like receptor (TLR) stimulation shifts intracellular metabolism toward glycolysis, while anti-inflammatory signals depend on enhanced mitochondrial respiration. How exogenous metabolic signals affect the immune response is unknown. We demonstrate that TLR-dependent responses of dendritic cells (DCs) are exacerbated by a high-fatty-acid (FA) metabolic environment. FAs suppress the TLR-induced hexokinase activity and perturb tricarboxylic acid cycle metabolism. These metabolic changes enhance mitochondrial reactive oxygen species (mtROS) production and, in turn, the unfolded protein response (UPR), leading to a distinct transcriptomic signature with IL-23 as hallmark. Interestingly, chemical or genetic suppression of glycolysis was sufficient to induce this specific immune response. Conversely, reducing mtROS production or DC-specific deficiency in XBP1 attenuated IL-23 expression and skin inflammation in an IL-23-dependent model of psoriasis. Thus, fine-tuning of innate immunity depends on optimization of metabolic demands and minimization of mtROS-induced UPR.</p>', 'date' => '2019-05-16', 'pmid' => 'http://www.pubmed.gov/31031005', 'doi' => '10.1016/j.cell.2019.03.018', 'modified' => '2019-08-06 16:57:45', 'created' => '2019-07-31 13:35:50', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '3422', 'name' => 'Aster Proteins Facilitate Nonvesicular Plasma Membrane to ER Cholesterol Transport in Mammalian Cells.', 'authors' => 'Sandhu J, Li S, Fairall L, Pfisterer SG, Gurnett JE, Xiao X, Weston TA, Vashi D, Ferrari A, Orozco JL, Hartman CL, Strugatsky D, Lee SD, He C, Hong C, Jiang H, Bentolila LA, Gatta AT, Levine TP, Ferng A, Lee R, Ford DA, Young SG, Ikonen E, Schwabe JWR, To', 'description' => '<p>The mechanisms underlying sterol transport in mammalian cells are poorly understood. In particular, how cholesterol internalized from HDL is made available to the cell for storage or modification is unknown. Here, we describe three ER-resident proteins (Aster-A, -B, -C) that bind cholesterol and facilitate its removal from the plasma membrane. The crystal structure of the central domain of Aster-A broadly resembles the sterol-binding fold of mammalian StARD proteins, but sequence differences in the Aster pocket result in a distinct mode of ligand binding. The Aster N-terminal GRAM domain binds phosphatidylserine and mediates Aster recruitment to plasma membrane-ER contact sites in response to cholesterol accumulation in the plasma membrane. Mice lacking Aster-B are deficient in adrenal cholesterol ester storage and steroidogenesis because of an inability to transport cholesterol from SR-BI to the ER. These findings identify a nonvesicular pathway for plasma membrane to ER sterol trafficking in mammals.</p>', 'date' => '2018-10-04', 'pmid' => 'http://www.pubmed.gov/30220461', 'doi' => '10.1016/j.cell.2018.08.033', 'modified' => '2018-12-31 11:27:24', 'created' => '2018-12-04 09:51:07', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '3506', 'name' => 'Development of PCR screening assays focused on genecoding sequences for GMO detection', 'authors' => 'Frédéric Debode, Éric Janssen, Gilbert Berben', 'description' => '<p>For the detection of genetically modifed organisms (GMO), screening is the frst step used to determine if a GMO or its derived products are present in food or feed. If the result is positive, additional tests must be done to identify and, where necessary, quantify the genetically modifed (GM) event present. The screening must be as wide as possible to cover the different possible GM events encountered on the market. In this paper, we present new real-time PCR screening methods (DNA-based methods) using hybridization probes (TaqMan probes) focused on gene-coding regions. These were selected on the basis of their occurrence in GM constructs (Block et al., 2013). Detection is also possible by searching for the new proteins using immunoassays (ELISA or immunochromatographic test strips). These techniques are well-suited for the detection of proteins produced by the EPSPS, pat, bar and cry genes (Stave, 2002; Van den Bulcke et al., 2007). However, this approach is only suitable for raw and unprocessed products. DNA-based methods were therefore preferred in this study. PCR assays focused on genes can be combined with those focused on promoters and terminators (Debode et al., 2013) in order to provide screening with wider coverage. The PCR assays developed in this paper were based on the sequences of the bar, pat, EPSPS, gox, gus and hsp70 genes.</p>', 'date' => '2018-09-19', 'pmid' => 'https://popups.uliege.be/1780-4507/index.php?id=16568', 'doi' => '10.25518/1780-4507.16568', 'modified' => '2022-05-18 18:42:38', 'created' => '2019-02-27 12:54:44', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '3461', 'name' => 'Development of Real-time PCR Assays for the Detection of the pin II Terminator (tpinII) Used in GM Constructs and Its Donor Organism, Potato (Solanum tuberosum)', 'authors' => 'Debode Frederic, Zdeňková Kamila, Janssen Eric, Tizolova Anette, du Jardin Patrick, Berben Gilbert, Demnerova Kateřina', 'description' => '<p>G-rich DNA sequences can form four-stranded G-quadruplex (G4) secondary structures and are linked to fundamental biological processes such as transcription, replication and telomere maintenance. G4s are also implicated in promoting genome instability, cancer and other diseases. Here, we describe a detailed G4 ChIP-seq method that robustly enables the determination of G4 structure formation genome-wide in chromatin. This protocol adapts traditional ChIP-seq for the detection of DNA secondary structures through the use of a G4-structure-specific single-chain antibody with refinements in chromatin immunoprecipitation followed by high-throughput sequencing. This technology does not require expression of the G4 antibody in situ, enabling broad applicability to theoretically all chromatin sources. Beginning with chromatin isolation and antibody preparation, the entire protocol can be completed in <1 week, including basic computational analysis.</p>', 'date' => '2018-02-23', 'pmid' => 'https://link.springer.com/article/10.1007/s12161-018-1203-4', 'doi' => '10.1007/s12161-018-1203-4', 'modified' => '2019-02-18 11:58:32', 'created' => '2019-02-14 15:01:22', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '3591', 'name' => 'Detection of Hermetia illucens by real-time PCR', 'authors' => 'Marien A., Debode F., Aerts C., Ancion C., Francis F., Berben G.', 'description' => '<p>Insects are rich in proteins and could be an alternative source of macronutrients to feed animals and humans. Over the past few years, numerous companies have started producing insects for feed purposes. In Europe, the processed animal proteins obtained from seven insect species have been authorised for aquaculture by Commission Regulation (EU) 2017/893 since 1 July 2017. Methods of authentication are required to check the conformity of the products. In this study, we propose a real-time PCR method for the specific detection of the black soldier fly (Hermetia illucens L.), one of the most widely used insects for feed production. The developed PCR assays amplify a 67 bp fragment based on the mitochondrial COX3 gene coding for subunit 3 of the cytochrome c oxidase. The qualitative method was tested according to several performance criteria. The specificity was tested against 51 insect species. The specificity was also checked against plant species and other animal species such as crustaceans, mammals and birds. The sensitivity, efficiency and robustness of the PCR test were successfully tested. The applicability of the test was proven through the analysis of real-life processed samples (industrial meals) of H. illucens</p>', 'date' => '2018-01-01', 'pmid' => 'https://www.wageningenacademic.com/doi/10.3920/JIFF2017.0069', 'doi' => '10.3920/JIFF2017.0069', 'modified' => '2022-05-18 16:48:45', 'created' => '2019-04-16 12:25:30', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '3620', 'name' => 'Impact of Lactobacillus plantarum ST-III on the composition of infant gut microbiota and its potential synergism with breast milk and infant formula as revealed by an in vitro study', 'authors' => 'Yan Minghui, Chen Wanyi, Li Nan, Ren Jing, Chen Chen, You Chunping, Liu Zhenmin', 'description' => '<p>Several studies have demonstrated the effect of probiotics on prevention of diseases in infants. However, the mechanisms remain not fully addressed. In the present study a modified standard ileal efflux medium (SIEM) was exploited in an in vitro analysis and the effect of Lactobacillus plantarum ST-III on the composition of infant gut microbiota was examined in I-chip analysis followed by qPCR confirmation. Also, the effect of ST-III, when administered in combination with breast milk and infant formula, was investigated. The results showed that ST-III could reduce the overall amount of Enterobacteria and Bacteroidetes bacteria, yet increase the abundance of Lactobacillus and certain Bifidobacterium species. Notably, some species/genus of bacteria of medical significance, e.g., Alcaligenaceae sutterella and Desulfovibrio bacteria were inhibited by ST-III. Moreover, co-administration of breast milk or infant formula could increase the abundance of ST-III, suggesting a synergistic effect between ST-III and infant formula and breast milk.</p>', 'date' => '2018-01-01', 'pmid' => 'https://www.sciencedirect.com/science/article/abs/pii/S0958694618301420', 'doi' => '10.1016/j.idairyj.2018.05.014', 'modified' => '2022-05-18 18:49:31', 'created' => '2019-04-16 13:01:51', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '2454', 'name' => 'Universal Mastermix SDS GB en', 'language' => 'en', 'url' => 'files/SDS/Universal_Mastermix/SDS-DMML-D2-D600-Universal_Mastermix-GB-en-1_0.pdf', 'countries' => 'GB', 'modified' => '2022-08-30 10:56:22', 'created' => '2022-08-30 10:56:22', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '2456', 'name' => 'Universal Mastermix SDS US en', 'language' => 'en', 'url' => 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nl', 'language' => 'nl', 'url' => 'files/SDS/Universal_Mastermix/SDS-DMML-D2-D600-Universal_Mastermix-BE-nl-1_0.pdf', 'countries' => 'BE', 'modified' => '2022-08-30 10:56:22', 'created' => '2022-08-30 10:56:22', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '2449', 'name' => 'Universal Mastermix SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/Universal_Mastermix/SDS-DMML-D2-D600-Universal_Mastermix-BE-fr-1_0.pdf', 'countries' => 'BE', 'modified' => '2022-08-30 10:56:22', 'created' => '2022-08-30 10:56:22', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '2453', 'name' => 'Universal Mastermix SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/Universal_Mastermix/SDS-DMML-D2-D600-Universal_Mastermix-FR-fr-1_0.pdf', 'countries' => 'FR', 'modified' => '2022-08-30 10:56:22', 'created' => '2022-08-30 10:56:22', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '2452', 'name' => 'Universal Mastermix SDS ES es', 'language' => 'es', 'url' => 'files/SDS/Universal_Mastermix/SDS-DMML-D2-D600-Universal_Mastermix-ES-es-1_0.pdf', 'countries' => 'ES', 'modified' => '2022-08-30 10:56:22', 'created' => '2022-08-30 10:56:22', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '<br/><small><span style="color:#CCC">(GMO-UN-A600)</span></small>' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '127', 'name' => 'Datasheet Universal Mastermix DMMLD2D600', 'description' => '<p>Datasheet description</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/Datasheet_Universal_Mastermix_DMMLD2D600.pdf', 'slug' => 'datasheet-universal-mastermix-DMMLD2D600', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-01-26 16:36:13', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( 'id' => '989', 'product_id' => '2642', 'document_id' => '127' ) ) $sds = array( 'id' => '2452', 'name' => 'Universal Mastermix SDS ES es', 'language' => 'es', 'url' => 'files/SDS/Universal_Mastermix/SDS-DMML-D2-D600-Universal_Mastermix-ES-es-1_0.pdf', 'countries' => 'ES', 'modified' => '2022-08-30 10:56:22', 'created' => '2022-08-30 10:56:22', 'ProductsSafetySheet' => array( 'id' => '4172', 'product_id' => '2642', 'safety_sheet_id' => '2452' ) ) $publication = array( 'id' => '3620', 'name' => 'Impact of Lactobacillus plantarum ST-III on the composition of infant gut microbiota and its potential synergism with breast milk and infant formula as revealed by an in vitro study', 'authors' => 'Yan Minghui, Chen Wanyi, Li Nan, Ren Jing, Chen Chen, You Chunping, Liu Zhenmin', 'description' => '<p>Several studies have demonstrated the effect of probiotics on prevention of diseases in infants. However, the mechanisms remain not fully addressed. In the present study a modified standard ileal efflux medium (SIEM) was exploited in an in vitro analysis and the effect of Lactobacillus plantarum ST-III on the composition of infant gut microbiota was examined in I-chip analysis followed by qPCR confirmation. Also, the effect of ST-III, when administered in combination with breast milk and infant formula, was investigated. The results showed that ST-III could reduce the overall amount of Enterobacteria and Bacteroidetes bacteria, yet increase the abundance of Lactobacillus and certain Bifidobacterium species. Notably, some species/genus of bacteria of medical significance, e.g., Alcaligenaceae sutterella and Desulfovibrio bacteria were inhibited by ST-III. Moreover, co-administration of breast milk or infant formula could increase the abundance of ST-III, suggesting a synergistic effect between ST-III and infant formula and breast milk.</p>', 'date' => '2018-01-01', 'pmid' => 'https://www.sciencedirect.com/science/article/abs/pii/S0958694618301420', 'doi' => '10.1016/j.idairyj.2018.05.014', 'modified' => '2022-05-18 18:49:31', 'created' => '2019-04-16 13:01:51', 'ProductsPublication' => array( 'id' => '3567', 'product_id' => '2642', 'publication_id' => '3620' ) ) $externalLink = ' <a href="https://www.sciencedirect.com/science/article/abs/pii/S0958694618301420" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
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