Oxford Nanopore R10.4 long-read sequencing enables the generation ofnear-finished bacterial genomes from pure cultures and metagenomes withoutshort-read or reference polishing.

Sereika Mantas et al.

Long-read Oxford Nanopore sequencing has democratized microbial genome sequencing and enables the recovery of highly contiguous microbial genomes from isolates or metagenomes. However, to obtain near-finished genomes it has been necessary to include short-read polishing to correct insertions and deletions derived from homopolymer regions. Here, we show that Oxford Nanopore R10.4 can be used to generate near-finished microbial genomes from isolates or metagenomes without short-read or reference polishing.

Oxford Nanopore R10.4 long-read sequencing enables the generation ofnear-finished bacterial genomes from pure cultures and metagenomes withoutshort-read or reference polishing.

Products used in this publication

Megaruptor® 2

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