We present a genome assembly from an individual female Bombus pratorum (the Early Bumblebee; Arthropoda; Insecta; Hymenoptera; Apidae). The genome sequence is 285.1 megabases in span. Most of the assembly is scaffolded into 18 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 21.5 kilobases in length. Gene annotation of this assembly on Ensembl identified 13,746 protein coding genes.