Cellular reprogramming in vivo initiated by SOX4 pioneer factor activity Published: February, 2024 Abstract:
Tissue damage elicits cell fate switching through a process called metaplasia, but how the starting cell fate is silenced and the new cell fate is activated has not been investigated in animals. In cell culture, pioneer transcription factors media... Read more »
The ncBAF complex regulates transcription in AML through H3K27ac sensing by BRD9 Published: December, 2023 Abstract:
The non-canonical BAF complex (ncBAF) subunit BRD9 is essential for acute myeloid leukemia (AML) cell viability but has an unclear role in leukemogenesis. Because BRD9 is required for ncBAF complex assembly through its DUF3512 domain, precise brom... Read more »
Combined Analysis of mRNA Expression and Open Chromatin in Microglia Published: August, 2023 Abstract:
The advance of single-cell RNA-sequencing technologies in the past years has enabled unprecedented insights into the complexity and heterogeneity of microglial cell states in the homeostatic and diseased brain. This includes rather complex proteom... Read more »
Volumetric imaging of an intact organism by a distributed molecular network Published: August, 2023 Abstract:
Lymphatic, nervous, and tumoral tissues, among others, exhibit physiology that emerges from three-dimensional interactions between genetically unique cells. A technology capable of volumetrically imaging transcriptomes, genotypes, and morphologies... Read more »
Spatial epigenome-transcriptome co-profiling of mammalian tissues. Published: April, 2023 Abstract:
Emerging spatial technologies, including spatial transcriptomics and spatial epigenomics, are becoming powerful tools for profiling of cellular states in the tissue context. However, current methods capture only one layer of omics information at a... Read more »
Physiological reprogramming in vivo mediated by Sox4 pioneer factoractivity Published: January, 2023 Abstract:
Tissue damage elicits cell fate switching through a process called metaplasia, but how the starting cell fate is silenced and the new cell fate is activated has not been investigated in animals. In cell culture, pioneer transcription factors media... Read more »
Optimized single-nucleus transcriptional profiling by combinatorialindexing. Published: October, 2022 Abstract:
Single-cell combinatorial indexing RNA sequencing (sci-RNA-seq) is a powerful method for recovering gene expression data from an exponentially scalable number of individual cells or nuclei. However, sci-RNA-seq is a complex protocol that has histo... Read more »
Spatial profiling of chromatin accessibility in mouse and human tissues Published: August, 2022 Abstract:
Cellular function in tissue is dependent on the local environment, requiring new methods for spatial mapping of biomolecules and cells in the tissue context1. The emergence of spatial transcriptomics has enabled genome-scale gene expression m... Read more »
