The first step of ChIP - chromatin preparation - plays a key role in a succesful ChIP experiment. Each step of chromatin preparation (starting amount of material, cross-linking, cell lysis and chromatin shearing) has to be optimized, leading to a chromatin sample suitable for ChIP. A high quality chromatin sample combines two features: a suitable fragment size range and the availability of epitopes for ChIP.
Diagenode has optimized a range of solutions for successful chromatin preparation. Chromatin EasyShear Kits together with the Bioruptor ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.
Diagenode’s Chromatin EasyShear Kits:
Chromatin preparation is the first step of a ChIP assay. At Diagenode we offer complete and optimized solutions for ChIP and ChIP-seq, which include all buffers for chromatin preparation. For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs:
| SAMPLE TYPE | SAMPLE INPUT | KIT | SDS CONCENTRATION |
NUCLEI ISOLATION |
||
|---|---|---|---|---|---|---|
 |
CELLS
|
< 100,000 | Chromatin EasyShear Kit High SDS |
1% |  |
|
|
CELLS
|
> 100,000 | Chromatin EasyShear Kit Ultra Low SDS |
< 0.1% |  |
||
|
TISSUE
|
Chromatin EasyShear Kit Ultra Low SDS |
< 0.1% | |
|||
|
PLANT TISSUE
|
Chromatin EasyShear Kit for Plant |
0.5% | |
|||
|
FFPE SAMPLES
|
Chromatin EasyShear Kit Low SDS |
0.2% | |
|||
 |
||||||
|
CELLS
|
Chromatin EasyShear Kit Low SDS |
0.2% | |
|||
|
TISSUE
|
||||||
|
FFPE SAMPLES
|
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| Cat. No. | Product | Format | Price | |
|---|---|---|---|---|
| C01020013 |
Chromatin EasyShear Kit - Low SDS Previous name of the kit: Chromatin shearing optimization kit – Low SDS (iDeal Kit for TFs) A high quality chromatin preparation is very complex and... |
4 chrom. prep./ 24 rxns | $345.00 | |
| C01020014 |
Chromatin EasyShear Kit for Plant Previous name of the kit: Chromatin Shearing Optimization Kit (Universal Plant ChIP-seq kit) The first critical step of a successful ChIP experiment is th... |
12 rxns | $345.00 | |
| C01020010 |
Chromatin EasyShear Kit – Ultra Low SDS Previous name of the kit: Chromatin shearing optimization kit – Ultra Low SDS A high quality chromatin preparation is very complex and requires a lo... |
10 chrom. prep./60 rxns | $345.00 | |
| C01020012 |
Chromatin EasyShear Kit - High SDS Previous name: Chromatin Shearing Optimization Kit - High SDS (True Micro ChIP kit) A high quality chromatin preparation is very complex and requires a lo... |
100 million cells | $345.00 |
