Chromatin studies

CUT&Tag

CUT&Tag-sequencing (Cleavage Under Targets and Tagmentation) is a new alternative method to ChIP-seq combining antibody-targeted controlled cleavage by a protein A-Tn5 fusion with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. At Diagenode we offer a complete solution for CUT&Tag – our iDeal CUT&Tag for Histones (developped for histone marks and some non-histone proteins), but also stand-alone fusion protein – pA-Tn5 Transposase. Moreover, we have validated our ChIP-seq grade antibodies in CUT&Tag proving their high performance in this assay.

How does it work?

The iDeal CUT&Tag protocol involves the binding of cells on a solid phase ConA magnetic beads, allowing magnetic handling of the cells for the major steps of the protocol. Bead-bound cells are permeabilized, incubated with primary antibody against a target of interest and secondary antibody. Then, Diagenode’s protein pA-Tn5 Transposase - loaded is bound to the complex. Protein A guides Tn5 transposase on chromatin to the antibody attached to its target. Tn5 transposase is activated by Mg+2 ions to insert the sequencing adaptors into genomic regions of interest. DNA is then purified and the tagmented genomic regions of interest are amplified by PCR using Diagenode’s Primer Indexes for tagmented libraries.

Products for CUT&Tag assay

Complete solutions
Fusion protein
- pA/Tn5 Transposase (loaded)
- pA/Tn5 Transposase (unloaded)
CUT&Tag grade antibodies
- Antibodies validated in CUT&Tag
- Check out our list of ChIP-seq grade antibodies
- Read more about the performance of Diagenode antibodies in CUT&Tag
Positive & Negative CUT&Tag control
- Antibody package for CUT&Tag (anti-rabbit)
- Antibody package for CUT&Tag (anti-mouse)
DNA purification

IPure kit v2
MicroChIP DiaPure columns

Sequencing indexes
- Primer indexes for tagmented libraries