ChIPmentation™

Benefits of the ChIPmentation system for optimal ChIP-seq

• Ensure high quality sequencing data
• Perform ChIP-seq on low input samples
• Enjoy an easy and fast protocol
• Benefit from the elimination of sequencing adaptor dimers
• Automate on the IP-Star® to support standardization and save time

• ChIPmentation workflow - Read more
  

  In traditional ChIP-seq library preparation, the ligation of the adaptors is performed after chromatin immunoprecipitation, in three steps. In the Diagenode ChIPmentation workflow, not only are the adaptors directly incorporated during ChIP, but also the main steps are automated on the Diagenode IP-Star®. The combination of direct adaptor incorporation and automation allows for higher sensitivity from low cell numbers and reproducibility of results.

Complete kits:

• µChIPmentation for Histones
  The Diagenode µChIPmentation Kit for Histones is optimized to perform ChIP-seq on as little as 10.000 cells from cell fixation to purified libraries.
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• ChIPmentation Kit for Histones
  ChIPmentation is a ligation-free, tagmentation-based protocol that incorporates chromatin immunoprecipitation with NGS library preparation.
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Modules:

• TAG Kit for ChIPmentation
  The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol.
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Primer indexes for tagmented libraries:

• 24 SI for ChIPmentation
  The 24 SI for ChIPmentation includes 24 single indexes compliting the TAG Kit for ChIPmentation.
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• 24 SI for Tagmented libraries
  The 24 SI for tagmented libraries includes 24 single primer indexes for multiplexing up to 24 samples. This set of indexes is compatible with any tagmentation-based library preparation protocols, such as ChIPmentation, ATAC-seq or CUT&Tag technologies.
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• 24 UDI for Tagmented libraries - Set I
  The 24 UDI (Unique dual indexes) for tagmented libraries – Set I provides combinations of barcodes where each barcode is uniquely attributed to one sample.
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• 24 UDI for Tagmented libraries - Set II
  The 24 UDI (Unique dual indexes) for tagmented libraries – Set II includes 24 primer pairs for unique dual-indexing and is designed to be combined with the 24 UDI for tagmented libraries – Set I, allowing multiplexing of up to 48 samples for sequencing on Illumina platforms.
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• 24 UDI for Tagmented libraries - Set III
  The 24 UDI (Unique dual indexes) for tagmented libraries – Set III includes 24 primer pairs for unique dual-indexing. It is designed to be used in combination with other sets of UDI (Set I, II and IV), allowing multiplexing of up to 96 samples for sequencing on Illumina platforms.
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• 
  
  The 24 UDI (Unique dual indexes) for tagmented libraries – Set IV includes 24 primer pairs for unique dual-indexing. It is designed to be used in combination with other sets of UDI (Set I, II and III), allowing multiplexing of up to 96 samples for sequencing on Illumina platforms.
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Separately available products:

• Tagmentase (Tn5 transposase) - loaded
  Diagenode Tagmentase – loaded is a hyperactive Tn5 transposase preloaded with Illumina-compatible sequencing adapters.
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• Tagmentase (Tn5 transposase) - unloaded
  Diagenode Tagmentase is a hyperactive Tn5 transposase with the ability to cut DNA and insert sequences of interest into any target DNA in one step. This enzyme is not loaded with DNA oligos.
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• Tagmentase Dilution Buffer
  Diagenode Tagmentase Dilution Buffer is recommended for dilution of Tagmentase (Cat. No. C01070010) before or after transposome assembly.
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• Tagmentation Buffer (1x)
  Diagenode Tagmentation Buffer (1x) is the recommended reagent to perform any tagmentation reactions.
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