The pattern of DNA modifications is critical for genome stability and the control of gene expression in the cell. Methylation of 5-cytosine (5-mC), one of the best-studied epigenetic marks, is carried out by the DNA methyltransferases DNMT3A and B and DNMT1. DNMT3A and DNMT3B are responsible for de novo DNA methylation, whereas DNMT1 maintains existing methylation. 5-mC undergoes active demethylation which is performed by the Ten-Eleven Translocation (TET) familly of DNA hydroxylases. The latter consists of 3 members TET1, 2 and 3. All 3 members catalyze the conversion of 5-methylcytosine (5-mC) into 5-hydroxymethylcytosine (5-hmC), and further into 5-formylcytosine (5-fC) and 5-carboxycytosine (5-caC). 5-fC and 5-caC can be converted to unmodified cytosine by Thymine DNA Glycosylase (TDG). It is not yet clear if 5-hmC, 5-fC and 5-caC have specific functions or are simply intermediates in the demethylation of 5-mC.
DNA methylation is generally considered as a repressive mark and is usually associated with gene silencing. It is essential that the balance between DNA methylation and demethylation is precisely maintained. Dysregulation of DNA methylation may lead to many different human diseases and is often observed in cancer cells.
Diagenode offers highly validated antibodies against different proteins involved in DNA modifications as well as against the modified bases allowing the study of all steps and intermediates in the DNA methylation/demethylation pathway:
Diagenode exclusively sources the original 5-methylcytosine monoclonal antibody (clone 33D3).
Check out the list below to see all proposed antibodies for DNA modifications.
Diagenode’s highly validated antibodies:
| Cat. No. | Product | Application | Purity | |
|---|---|---|---|---|
| C15200003 |
5-methylcytosine (5-mC) Antibody for ICC/IF (50 μg/39 μl) |
DB FISH IF | Protein A purified monoclonal antibody. | |
| C15410209 |
3-methylcytosine (3-mC) Antibody (50 μg/28 μl) |
DB | Protein G purified | |
| C15410204-100 |
5-Carboxylcytosine (5-caC) Antibody (100 µg) |
DB IF IP | Affinity purified | |
| C15310200 |
5-formylcytosine (5-fC) Antibody (100 µl) |
DIP ELISA | Whole antiserum | |
| C15200200 |
5-hydroxymethylcytosine (5-hmC) Antibody (mouse) (50 µg/50 µl) |
DB ELISA | Protein G purified | |
| C15410205 |
5-hydroxymethylcytosine (5-hmC) Antibody (rabbit) (50 μg) |
DB ELISA Methylated DNA immunoprecipitation | Affinity purified | |
| C15310210-100 |
5-hydroxymethylcytosine (5-hmC) Antibody (rabbit) (100 µl) |
DB ELISA | Whole antiserum | |
| C15220001 |
5-hydroxymethylcytosine (5-hmC) Antibody (rat) (50 µg) |
DB ELISA | Affinity purified | |
| C15200006-100 |
5-methylcytosine (5-mC) Antibody - cl. b (100 µg) |
IF Methylated DNA immunoprecipitation | Monoclonal antibody purified by gel filtration. | |
| C15200081-100 |
5-methylcytosine (5-mC) Antibody - clone 33D3 (100 µg) |
DB IF Methylated DNA immunoprecipitation | Protein A purified monoclonal antibody. | |
| C15310111 |
DNMT1 Antibody (100 µl) |
ELISA WB | Whole antiserum | |
| C15410084 |
DNMT3A Antibody (50 µg/67 µl) |
ELISA IP WB | Affinity purified | |
| C15410085 |
DNMT3A Antibody (50 µg/54 µl) |
ELISA IP WB | Affinity purified | |
| C15410086 |
DNMT3A Antibody (50 µg/64 µl) |
ELISA IP WB | Affinity purified | |
| C15410218 |
DNMT3B Antibody (50 μg/ 16 μl) |
ELISA IF WB | Affinity purified | |
| C15310044 |
DNMT3B Antibody (100 µl) |
ELISA WB | Whole antiserum | |
| C15200227 |
TDG Antibody (50 μg) |
IF WB | Affinity purified | |
| C15200179 |
TET2 Antibody (50 μg/50 μl) |
IP WB | Protein G purified | |
| C15410255-100 |
TET2 Antibody (100 μl) |
ChIP-qPCR IF IP WB | Affinity purified | |
| C15410311 |
TET3 Antibody (50 μg) |
ELISA WB | Affinity purified |
