Methylated DNA Immunoprecipitation Analysis of Mammalian Endogenous Retroviruses. Published: February, 2016 Abstract:
Endogenous retroviruses are repetitive sequences found abundantly in mammalian genomes which are capable of modulating host gene expression. Nevertheless, most endogenous retrovirus copies are under tight epigenetic control via histone-repressive ... Read more »
Role of Growth Arrest and DNA Damage-Inducible, Beta in Alcohol-Drinking Behaviors Published: February, 2016 Abstract:
BACKGROUND:
The contribution of epigenetic factors, such as histone acetylation and DNA methylation, to the regulation of alcohol-drinking behavior has been increasingly recognized over the last several years. GADD45b is a protein demonstrated to... Read more »
Protocol for Methylated DNA Immunoprecipitation (MeDIP) Analysis Published: February, 2016 Abstract:
DNA methylation is a fundamental epigenetic mechanism for silencing gene expression by either modifying chromatin structure to a repressive state or interfering with the transcription factors’ binding. DNA methylation primarily occurs at the... Read more »
De novo DNA methylation drives 5hmC accumulation in mouse zygotes Published: January, 2016 Abstract:
Zygotic epigenetic reprogramming entails genome-wide DNA demethylation that is accompanied by Tet methylcytosine dioxygenase 3 (Tet3)-driven oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC; refs ,,,). Here we demonstrate ... Read more »
Targeted disruption of DNMT1, DNMT3A and DNMT3B in human embryonic stem cells. Published: May, 2015 Abstract:
DNA methylation is a key epigenetic modification involved in regulating gene expression and maintaining genomic integrity. Here we inactivated all three catalytically active DNA methyltransferases (DNMTs) in human embryonic stem cells (ESCs) using... Read more »
Naive pluripotency is associated with global DNA hypomethylation. Published: March, 2013 Abstract:
Naive pluripotent embryonic stem cells (ESCs) and embryonic germ cells (EGCs) are derived from the preimplantation epiblast and primordial germ cells (PGCs), respectively. We investigated whether differences exist between ESCs and EGCs, in view of... Read more »
Association of UHRF1 with methylated H3K9 directs the maintenance of DNA methylation. Published: September, 2012 Abstract:
A fundamental challenge in mammalian biology has been the elucidation of mechanisms linking DNA methylation and histone post-translational modifications. Human UHRF1 (ubiquitin-like PHD and RING finger domain-containing 1) has multiple domains tha... Read more »
Epigenetic silencing mediated through activated PI3K/AKT signaling in breast cancer. Published: March, 2011 Abstract:
Trimethylation of histone 3 lysine 27 (H3K27me3) is a critical epigenetic mark for the maintenance of gene silencing. Additional accumulation of DNA methylation in target loci is thought to cooperatively support this epigenetic silencing during tu... Read more »
Methylated DNA Immunoprecipitation (MeDIP) from Low Amounts of Cells. Abstract:
Methylated DNA immunoprecipitation (MeDIP) is an immunocapturing approach for unbiased enrichment of DNA that is methylated on cytosines. The principle is that genomic DNA is randomly sheared by sonication and immunoprecipitated with an antibody t... Read more »
