Epigenetic Drifts during Long-Term Intestinal OrganoidCulture Published: July, 2021 Abstract:
Organoids retain the morphological and molecular patterns of their tissue of origin, are self-organizing, relatively simple to handle and accessible to genetic engineering. Thus, they represent an optimal tool for studying the mechanisms of tissue... Read more »
Measuring Histone Modifications in the Human Parasite Schistosoma mansoni Published: May, 2020 Abstract:
DNA-binding proteins play critical roles in many major processes such as development and sexual biology of Schistosoma mansoni and are important for the pathogenesis of schistosomiasis. Chromatin immunoprecipitation (ChIP) experiments followed by ... Read more »
APRDX1 mutant allele causes a MMACHC secondary epimutation in cblC patients Published: January, 2018 Abstract:
To date, epimutations reported in man have been somatic and erased in germlines. Here, we identify a cause of the autosomal recessive cblC class of inborn errors of vitamin B12 metabolism that we name “epi-cblC”. The subjects are compo... Read more »
Crebbp loss cooperates with Bcl2 over-expression to promote lymphoma in mice Published: March, 2017 Abstract:
CREBBP is targeted by inactivating mutations in follicular lymphoma (FL) and diffuse large B-cell lymphoma (DLBCL). Here, we provide evidence from transgenic mouse models that Crebbp deletion results in deficits in B-cell development and can coope... Read more »
DNMT3L enables accumulation and inheritance of epimutations in transgenic Drosophila Published: January, 2016 Abstract:
DNMT3L is an important epigenetic regulator in mammals, integrating DNA methylation and histone modification based epigenetic circuits. Here we show DNMT3L to be a part of the machinery that enables inheritance of epigenetic modifications from one... Read more »
Transcription Factor Repertoire of Homeostatic Eosinophilopoiesis Published: September, 2015 Abstract:
The production of mature eosinophils (Eos) is a tightly orchestrated process with the aim to sustain normal Eos levels in tissues while also maintaining low numbers of these complex and sensitive cells in the blood. To identify regulators of homeo... Read more »
Bacterial Infection Remodels the DNA Methylation Landscape of Human Dendritic Cells Published: March, 2015 Abstract:
DNA methylation is thought to be robust to environmental perturbations on a short time scale. Here, we challenge that view by demonstrating that the infection of human dendritic cells (DCs) with a pathogenic bacteria is associated with rapid chang... Read more »
Long noncoding RNA modulates alternative splicing regulators in Arabidopsis. Published: July, 2014 Abstract:
Alternative splicing (AS) of pre-mRNA represents a major mechanism underlying increased transcriptome and proteome complexity. Here, we show that the nuclear speckle RNA-binding protein (NSR) and the AS competitor long noncoding RNA (or ASCO-lncRN... Read more »
Early aberrant DNA methylation events in a mouse model of acute myeloid leukemia. Published: April, 2014 Abstract:
BACKGROUND: Aberrant DNA methylation is frequently found in human malignancies including acute myeloid leukemia (AML). While most studies focus on later disease stages, the onset of aberrant DNA methylation events and their dynamics during leukemi... Read more »
Differential methylation of the TRPA1 promoter in pain sensitivity. Published: January, 2014 Abstract:
Chronic pain is a global public health problem, but the underlying molecular mechanisms are not fully understood. Here we examine genome-wide DNA methylation, first in 50 identical twins discordant for heat pain sensitivity and then in 50 further ... Read more »
DNA methylation analysis of murine hematopoietic side population cells during aging. Published: October, 2013 Abstract:
Stem cells have been found in most tissues/organs. These somatic stem cells produce replacements for lost and damaged cells, and it is not completely understood how this regenerative capacity becomes diminished during aging. To study the possible ... Read more »
Integrated virus-host methylome analysis in head and neck squamous cell carcinoma Published: September, 2013 Abstract:
One in six cancers worldwide is caused by infection and human papillomavirus (HPV) is one of the main culprits. To better understand the dynamics of HPV integration and its effect on both the viral and host methylomes, we conducted whole-genome DN... Read more »
Nano-MeDIP-seq: Methylome analysis using low DNA concentrations (Prot 58) Published: December, 2012 Abstract:
To understand the functional consequences of DNA methylation on phenotypic plasticity, a genome - wide analysis should be embraced. This in turn requires a technique that balances accuracy, genome coverage, resolution and cost, yet is low in DNA i... Read more »
Methylome analysis using MeDIP-seq with low DNA concentrations. Published: March, 2012 Abstract:
DNA methylation is an epigenetic mark that has a crucial role in many biological processes. To understand the functional consequences of DNA methylation on phenotypic plasticity, a genome-wide analysis should be embraced. This in turn requires a t... Read more »
Whole-genome DNA methylation profiling using MethylCap-seq. Published: November, 2010 Abstract:
MethylCap-seq is a robust procedure for genome-wide profiling of DNA methylation. The approach consists of the capture of methylated DNA using the MBD domain of MeCP2, and subsequent next-generation sequencing of eluted DNA. Elution of the capture... Read more »
Quantitative comparison of genome-wide DNA methylation mapping technologies. Published: October, 2010 Abstract:
DNA methylation plays a key role in regulating eukaryotic gene expression. Although mitotically heritable and stable over time, patterns of DNA methylation frequently change in response to cell differentiation, disease and environmental influences... Read more »
PML-RARalpha/RXR Alters the Epigenetic Landscape in Acute Promyelocytic Leukemia. Published: February, 2010 Abstract:
Many different molecular mechanisms have been associated with PML-RARalpha-dependent transformation of hematopoietic progenitors. Here, we identified high confidence PML-RARalpha binding sites in an acute promyelocytic leukemia (APL) cell line and... Read more »
AutoMeDIP-seq: A high-throughput, whole genome, DNA methylation assay Abstract:
DNA methylation is an epigenetic mark linking DNA sequence and transcription regulation, and therefore plays an important role in phenotypic plasticity. The ideal whole genome methylation (methylome) assay should be accurate, affordable, high-thro... Read more »
Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation. Abstract:
Normal DNA methylation is an epigenetic modification required for proper development. Aberrant DNA methylation, in contrast, is frequently observed in many different malignancies including leukemias and lymphomas. Global DNA methylation profiling ... Read more »
