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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against m1A</strong><br />Immunoprecipitation was performed on 40 µg total RNA isolated from HEK293 cells using 15 µg of the Diagenode monoclonal antibody against m1A (cat. No. C15200235) or with an equal amount of mouse IgG2b, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the m1A antibody (upper right). The lower figure shows the gel image for the input, the negative IgG2b control and the m1A antibody (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Immunofluorescence using the Diagenode monoclonal antibody directed against m1A</strong><br />HeLa cells were stained with the Diagenode monoclonal antibody against m1A (cat. No. C15200235). Cells were fixed with 4% formaldehyde for 20 min at RT, permeabilized with 0.5% Triton X-100 for 5 min at RT and blocked with PBS containing 1% BSA. The cells were immunofluorescently labeled with the m1A antibody (left) diluted 1:1,000 in blocking solution followed by a goat anti-mouse antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with Hoechst33342. A merge of the two stainings is shown on the right.</small></p>
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<p><small><strong>Figure 3. Immunohistochemistry using the Diagenode monoclonal antibody directed against m1A</strong><br />Paraffin-embedded mouse liver tissue was analysed by immunohistochemical analysis using the Diagenode monoclonal antibody against m1A (cat. No. C15200235) diluted 1:10,000 (brown). The slides were counterstained with Hematoxylin (blue).</small></p>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against m1A</strong><br />Immunoprecipitation was performed on 40 µg total RNA isolated from HEK293 cells using 15 µg of the Diagenode monoclonal antibody against m1A (cat. No. C15200235) or with an equal amount of mouse IgG2b, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the m1A antibody (upper right). The lower figure shows the gel image for the input, the negative IgG2b control and the m1A antibody (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Immunofluorescence using the Diagenode monoclonal antibody directed against m1A</strong><br />HeLa cells were stained with the Diagenode monoclonal antibody against m1A (cat. No. C15200235). Cells were fixed with 4% formaldehyde for 20 min at RT, permeabilized with 0.5% Triton X-100 for 5 min at RT and blocked with PBS containing 1% BSA. The cells were immunofluorescently labeled with the m1A antibody (left) diluted 1:1,000 in blocking solution followed by a goat anti-mouse antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with Hoechst33342. A merge of the two stainings is shown on the right.</small></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
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<p><small><strong>Figure 2. Immunofluorescence using the Diagenode monoclonal antibody directed against m1A</strong><br />HeLa cells were stained with the Diagenode monoclonal antibody against m1A (cat. No. C15200235). Cells were fixed with 4% formaldehyde for 20 min at RT, permeabilized with 0.5% Triton X-100 for 5 min at RT and blocked with PBS containing 1% BSA. The cells were immunofluorescently labeled with the m1A antibody (left) diluted 1:1,000 in blocking solution followed by a goat anti-mouse antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with Hoechst33342. A merge of the two stainings is shown on the right.</small></p>
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<p><small><strong>Figure 3. Immunohistochemistry using the Diagenode monoclonal antibody directed against m1A</strong><br />Paraffin-embedded mouse liver tissue was analysed by immunohistochemical analysis using the Diagenode monoclonal antibody against m1A (cat. No. C15200235) diluted 1:10,000 (brown). The slides were counterstained with Hematoxylin (blue).</small></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against m1A</strong><br />Immunoprecipitation was performed on 40 µg total RNA isolated from HEK293 cells using 15 µg of the Diagenode monoclonal antibody against m1A (cat. No. C15200235) or with an equal amount of mouse IgG2b, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the m1A antibody (upper right). The lower figure shows the gel image for the input, the negative IgG2b control and the m1A antibody (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Immunofluorescence using the Diagenode monoclonal antibody directed against m1A</strong><br />HeLa cells were stained with the Diagenode monoclonal antibody against m1A (cat. No. C15200235). Cells were fixed with 4% formaldehyde for 20 min at RT, permeabilized with 0.5% Triton X-100 for 5 min at RT and blocked with PBS containing 1% BSA. The cells were immunofluorescently labeled with the m1A antibody (left) diluted 1:1,000 in blocking solution followed by a goat anti-mouse antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with Hoechst33342. A merge of the two stainings is shown on the right.</small></p>
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<p><small><strong>Figure 3. Immunohistochemistry using the Diagenode monoclonal antibody directed against m1A</strong><br />Paraffin-embedded mouse liver tissue was analysed by immunohistochemical analysis using the Diagenode monoclonal antibody against m1A (cat. No. C15200235) diluted 1:10,000 (brown). The slides were counterstained with Hematoxylin (blue).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20°C, permeabilized with acetone at -20°C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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'name' => '1-methyladenosine monoclonal antibody',
'description' => '<p><strong>Other names:</strong> m1A, 1mA, m1Ado</p><p>Monoclonal antibody raised in mouse against 1-methyladenosine (m1A) conjugated to KLH.</p>',
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<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15200235-ip-1.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against m1A</strong><br />Immunoprecipitation was performed on 40 µg total RNA isolated from HEK293 cells using 15 µg of the Diagenode monoclonal antibody against m1A (cat. No. C15200235) or with an equal amount of mouse IgG2b, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the m1A antibody (upper right). The lower figure shows the gel image for the input, the negative IgG2b control and the m1A antibody (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<div class="small-12 columns">
<p><small><strong>Figure 2. Immunofluorescence using the Diagenode monoclonal antibody directed against m1A</strong><br />HeLa cells were stained with the Diagenode monoclonal antibody against m1A (cat. No. C15200235). Cells were fixed with 4% formaldehyde for 20 min at RT, permeabilized with 0.5% Triton X-100 for 5 min at RT and blocked with PBS containing 1% BSA. The cells were immunofluorescently labeled with the m1A antibody (left) diluted 1:1,000 in blocking solution followed by a goat anti-mouse antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with Hoechst33342. A merge of the two stainings is shown on the right.</small></p>
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<div class="small-8 columns">
<p><small><strong>Figure 3. Immunohistochemistry using the Diagenode monoclonal antibody directed against m1A</strong><br />Paraffin-embedded mouse liver tissue was analysed by immunohistochemical analysis using the Diagenode monoclonal antibody against m1A (cat. No. C15200235) diluted 1:10,000 (brown). The slides were counterstained with Hematoxylin (blue).</small></p>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against m1A</strong><br />Immunoprecipitation was performed on 40 µg total RNA isolated from HEK293 cells using 15 µg of the Diagenode monoclonal antibody against m1A (cat. No. C15200235) or with an equal amount of mouse IgG2b, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the m1A antibody (upper right). The lower figure shows the gel image for the input, the negative IgG2b control and the m1A antibody (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Immunofluorescence using the Diagenode monoclonal antibody directed against m1A</strong><br />HeLa cells were stained with the Diagenode monoclonal antibody against m1A (cat. No. C15200235). Cells were fixed with 4% formaldehyde for 20 min at RT, permeabilized with 0.5% Triton X-100 for 5 min at RT and blocked with PBS containing 1% BSA. The cells were immunofluorescently labeled with the m1A antibody (left) diluted 1:1,000 in blocking solution followed by a goat anti-mouse antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with Hoechst33342. A merge of the two stainings is shown on the right.</small></p>
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<p><small><strong>Figure 3. Immunohistochemistry using the Diagenode monoclonal antibody directed against m1A</strong><br />Paraffin-embedded mouse liver tissue was analysed by immunohistochemical analysis using the Diagenode monoclonal antibody against m1A (cat. No. C15200235) diluted 1:10,000 (brown). The slides were counterstained with Hematoxylin (blue).</small></p>
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<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
×