1-methyladenosine Antibody

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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against m1A</strong><br />Immunoprecipitation was performed on 40 &micro;g total RNA isolated from HEK293 cells using 15 &micro;g of the Diagenode monoclonal antibody against m1A (cat. No. C15200235) or with an equal amount of mouse IgG2b, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the m1A antibody (upper right). The lower figure shows the gel image for the input, the negative IgG2b control and the m1A antibody (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Immunofluorescence using the Diagenode monoclonal antibody directed against m1A</strong><br />HeLa cells were stained with the Diagenode monoclonal antibody against m1A (cat. No. C15200235). Cells were fixed with 4% formaldehyde for 20 min at RT, permeabilized with 0.5% Triton X-100 for 5 min at RT and blocked with PBS containing 1% BSA. The cells were immunofluorescently labeled with the m1A antibody (left) diluted 1:1,000 in blocking solution followed by a goat anti-mouse antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with Hoechst33342. A merge of the two stainings is shown on the right.</small></p>
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<p><small><strong>Figure 3. Immunohistochemistry using the Diagenode monoclonal antibody directed against m1A</strong><br />Paraffin-embedded mouse liver tissue was analysed by immunohistochemical analysis using the Diagenode monoclonal antibody against m1A (cat. No. C15200235) diluted 1:10,000 (brown). The slides were counterstained with Hematoxylin (blue).</small></p>
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<td>Fig 1</td>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against m1A</strong><br />Immunoprecipitation was performed on 40 &micro;g total RNA isolated from HEK293 cells using 15 &micro;g of the Diagenode monoclonal antibody against m1A (cat. No. C15200235) or with an equal amount of mouse IgG2b, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the m1A antibody (upper right). The lower figure shows the gel image for the input, the negative IgG2b control and the m1A antibody (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Immunofluorescence using the Diagenode monoclonal antibody directed against m1A</strong><br />HeLa cells were stained with the Diagenode monoclonal antibody against m1A (cat. No. C15200235). Cells were fixed with 4% formaldehyde for 20 min at RT, permeabilized with 0.5% Triton X-100 for 5 min at RT and blocked with PBS containing 1% BSA. The cells were immunofluorescently labeled with the m1A antibody (left) diluted 1:1,000 in blocking solution followed by a goat anti-mouse antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with Hoechst33342. A merge of the two stainings is shown on the right.</small></p>
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<p><small><strong>Figure 3. Immunohistochemistry using the Diagenode monoclonal antibody directed against m1A</strong><br />Paraffin-embedded mouse liver tissue was analysed by immunohistochemical analysis using the Diagenode monoclonal antibody against m1A (cat. No. C15200235) diluted 1:10,000 (brown). The slides were counterstained with Hematoxylin (blue).</small></p>
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<td>IP</td>
<td>15 &mu;g per IP</td>
<td>Fig 1</td>
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<td>IF</td>
<td>1:1,000</td>
<td>Fig 2</td>
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	'created' => '2015-07-08 13:49:03',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]
Code Context
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$dataForView = array(
	'language' => 'en',
	'meta_keywords' => '',
	'meta_description' => '1-methyladenosine Monoclonal Antibody validated  in IP, IF and IHC. Batch-specific data available on the website. Other names: m1A, 1mA, m1Ado.',
	'meta_title' => '1-methyladenosine Monoclonal Antibody | Diagenode',
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			'id' => '2968',
			'antibody_id' => '526',
			'name' => '1-methyladenosine Antibody',
			'description' => '<p><strong>Other names:</strong> m1A, 1mA, m1Ado</p>
<p>Monoclonal antibody raised in mouse against 1-methyladenosine (m1A) conjugated to KLH.</p>',
			'label1' => 'Validation data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15200235-ip-1.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against m1A</strong><br />Immunoprecipitation was performed on 40 &micro;g total RNA isolated from HEK293 cells using 15 &micro;g of the Diagenode monoclonal antibody against m1A (cat. No. C15200235) or with an equal amount of mouse IgG2b, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the m1A antibody (upper right). The lower figure shows the gel image for the input, the negative IgG2b control and the m1A antibody (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<div class="row">
<div class="small-12 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15200235-if.jpg" alt="IF" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. Immunofluorescence using the Diagenode monoclonal antibody directed against m1A</strong><br />HeLa cells were stained with the Diagenode monoclonal antibody against m1A (cat. No. C15200235). Cells were fixed with 4% formaldehyde for 20 min at RT, permeabilized with 0.5% Triton X-100 for 5 min at RT and blocked with PBS containing 1% BSA. The cells were immunofluorescently labeled with the m1A antibody (left) diluted 1:1,000 in blocking solution followed by a goat anti-mouse antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with Hoechst33342. A merge of the two stainings is shown on the right.</small></p>
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<div class="small-8 columns">
<p><small><strong>Figure 3. Immunohistochemistry using the Diagenode monoclonal antibody directed against m1A</strong><br />Paraffin-embedded mouse liver tissue was analysed by immunohistochemical analysis using the Diagenode monoclonal antibody against m1A (cat. No. C15200235) diluted 1:10,000 (brown). The slides were counterstained with Hematoxylin (blue).</small></p>
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			'name' => '1-methyladenosine monoclonal antibody',
			'description' => 'N1-methyladenosine (m1A) is one of the major modified nucleosides present in most eukaryotic tRNAs but also has been found yeast rRNAs. The presence of the m1A modification in tRNA’s influences the structural stability and its binding to the polysome during translation. m1A is elevated in the urinary excretion of patients with various cancers and AIDS. Increased serum levels of m1A are also detected under stress conditions.',
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			'reactivity' => 'Human, mouse, other (wide range): positive.',
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			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
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<tbody>
<tr>
<td>IP</td>
<td>15 &mu;g per IP</td>
<td>Fig 1</td>
</tr>
<tr>
<td>IF</td>
<td>1:1,000</td>
<td>Fig 2</td>
</tr>
<tr>
<td>IHC</td>
<td>1:10,000</td>
<td>Fig 3</td>
</tr>
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			'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
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	'Product' => array(
		'id' => '2968',
		'antibody_id' => '526',
		'name' => '1-methyladenosine Antibody',
		'description' => '<p><strong>Other names:</strong> m1A, 1mA, m1Ado</p>
<p>Monoclonal antibody raised in mouse against 1-methyladenosine (m1A) conjugated to KLH.</p>',
		'label1' => 'Validation data',
		'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15200235-ip-1.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/c15200235-ip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-6 columns">
<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against m1A</strong><br />Immunoprecipitation was performed on 40 &micro;g total RNA isolated from HEK293 cells using 15 &micro;g of the Diagenode monoclonal antibody against m1A (cat. No. C15200235) or with an equal amount of mouse IgG2b, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the m1A antibody (upper right). The lower figure shows the gel image for the input, the negative IgG2b control and the m1A antibody (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<div class="small-12 columns">
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<p><small><strong>Figure 2. Immunofluorescence using the Diagenode monoclonal antibody directed against m1A</strong><br />HeLa cells were stained with the Diagenode monoclonal antibody against m1A (cat. No. C15200235). Cells were fixed with 4% formaldehyde for 20 min at RT, permeabilized with 0.5% Triton X-100 for 5 min at RT and blocked with PBS containing 1% BSA. The cells were immunofluorescently labeled with the m1A antibody (left) diluted 1:1,000 in blocking solution followed by a goat anti-mouse antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with Hoechst33342. A merge of the two stainings is shown on the right.</small></p>
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<p><small><strong>Figure 3. Immunohistochemistry using the Diagenode monoclonal antibody directed against m1A</strong><br />Paraffin-embedded mouse liver tissue was analysed by immunohistochemical analysis using the Diagenode monoclonal antibody against m1A (cat. No. C15200235) diluted 1:10,000 (brown). The slides were counterstained with Hematoxylin (blue).</small></p>
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		'description' => 'N1-methyladenosine (m1A) is one of the major modified nucleosides present in most eukaryotic tRNAs but also has been found yeast rRNAs. The presence of the m1A modification in tRNA’s influences the structural stability and its binding to the polysome during translation. m1A is elevated in the urinary excretion of patients with various cancers and AIDS. Increased serum levels of m1A are also detected under stress conditions.',
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<th>Suggested dilution</th>
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<td>IP</td>
<td>15 &mu;g per IP</td>
<td>Fig 1</td>
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<tr>
<td>IF</td>
<td>1:1,000</td>
<td>Fig 2</td>
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<td>IHC</td>
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<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s large range of highly validated antibodies includes the antibodies to study RNA modifications. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA modifications studies below.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]
Code Context
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	'meta_description' => '1-methyladenosine Monoclonal Antibody validated  in IP, IF and IHC. Batch-specific data available on the website. Other names: m1A, 1mA, m1Ado.',
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			'antibody_id' => '526',
			'name' => '1-methyladenosine Antibody',
			'description' => '<p><strong>Other names:</strong> m1A, 1mA, m1Ado</p>
<p>Monoclonal antibody raised in mouse against 1-methyladenosine (m1A) conjugated to KLH.</p>',
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<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15200235-ip-1.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/c15200235-ip-2.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<div class="small-6 columns">
<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against m1A</strong><br />Immunoprecipitation was performed on 40 &micro;g total RNA isolated from HEK293 cells using 15 &micro;g of the Diagenode monoclonal antibody against m1A (cat. No. C15200235) or with an equal amount of mouse IgG2b, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the m1A antibody (upper right). The lower figure shows the gel image for the input, the negative IgG2b control and the m1A antibody (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<div class="row">
<div class="small-12 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15200235-if.jpg" alt="IF" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. Immunofluorescence using the Diagenode monoclonal antibody directed against m1A</strong><br />HeLa cells were stained with the Diagenode monoclonal antibody against m1A (cat. No. C15200235). Cells were fixed with 4% formaldehyde for 20 min at RT, permeabilized with 0.5% Triton X-100 for 5 min at RT and blocked with PBS containing 1% BSA. The cells were immunofluorescently labeled with the m1A antibody (left) diluted 1:1,000 in blocking solution followed by a goat anti-mouse antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with Hoechst33342. A merge of the two stainings is shown on the right.</small></p>
</div>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15200235-ihc.jpg" alt="IHC" style="display: block; margin-left: auto; margin-right: auto;" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 3. Immunohistochemistry using the Diagenode monoclonal antibody directed against m1A</strong><br />Paraffin-embedded mouse liver tissue was analysed by immunohistochemical analysis using the Diagenode monoclonal antibody against m1A (cat. No. C15200235) diluted 1:10,000 (brown). The slides were counterstained with Hematoxylin (blue).</small></p>
</div>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against m1A</strong><br />Immunoprecipitation was performed on 40 &micro;g total RNA isolated from HEK293 cells using 15 &micro;g of the Diagenode monoclonal antibody against m1A (cat. No. C15200235) or with an equal amount of mouse IgG2b, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the m1A antibody (upper right). The lower figure shows the gel image for the input, the negative IgG2b control and the m1A antibody (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Immunofluorescence using the Diagenode monoclonal antibody directed against m1A</strong><br />HeLa cells were stained with the Diagenode monoclonal antibody against m1A (cat. No. C15200235). Cells were fixed with 4% formaldehyde for 20 min at RT, permeabilized with 0.5% Triton X-100 for 5 min at RT and blocked with PBS containing 1% BSA. The cells were immunofluorescently labeled with the m1A antibody (left) diluted 1:1,000 in blocking solution followed by a goat anti-mouse antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with Hoechst33342. A merge of the two stainings is shown on the right.</small></p>
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<p><small><strong>Figure 3. Immunohistochemistry using the Diagenode monoclonal antibody directed against m1A</strong><br />Paraffin-embedded mouse liver tissue was analysed by immunohistochemical analysis using the Diagenode monoclonal antibody against m1A (cat. No. C15200235) diluted 1:10,000 (brown). The slides were counterstained with Hematoxylin (blue).</small></p>
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	'modified' => '2016-01-13 12:23:53',
	'created' => '2015-07-08 13:49:03',
	'locale' => 'eng'
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$name = 'IHC'
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	'name' => '1-methyladenosine monoclonal antibody',
	'description' => '<p>Monoclonal antibody raised in mouse against 1-methyladenosine (m1A) conjugated to KLH.</p>',
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	'type' => 'Datasheet',
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	'modified' => '2018-05-09 15:38:51',
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	'name' => '1-methyladenosine antibody SDS ES es',
	'language' => 'es',
	'url' => 'files/SDS/1-methyladenosine/SDS-C15200235-1-methyladenosine_Antibody-ES-es-GHS_2_0.pdf',
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	'modified' => '2021-01-26 16:23:34',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]
Code Context
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$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'en',
	'meta_keywords' => '',
	'meta_description' => '1-methyladenosine Monoclonal Antibody validated  in IP, IF and IHC. Batch-specific data available on the website. Other names: m1A, 1mA, m1Ado.',
	'meta_title' => '1-methyladenosine Monoclonal Antibody | Diagenode',
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			'id' => '2968',
			'antibody_id' => '526',
			'name' => '1-methyladenosine Antibody',
			'description' => '<p><strong>Other names:</strong> m1A, 1mA, m1Ado</p>
<p>Monoclonal antibody raised in mouse against 1-methyladenosine (m1A) conjugated to KLH.</p>',
			'label1' => 'Validation data',
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<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15200235-ip-1.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against m1A</strong><br />Immunoprecipitation was performed on 40 &micro;g total RNA isolated from HEK293 cells using 15 &micro;g of the Diagenode monoclonal antibody against m1A (cat. No. C15200235) or with an equal amount of mouse IgG2b, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the m1A antibody (upper right). The lower figure shows the gel image for the input, the negative IgG2b control and the m1A antibody (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. Immunofluorescence using the Diagenode monoclonal antibody directed against m1A</strong><br />HeLa cells were stained with the Diagenode monoclonal antibody against m1A (cat. No. C15200235). Cells were fixed with 4% formaldehyde for 20 min at RT, permeabilized with 0.5% Triton X-100 for 5 min at RT and blocked with PBS containing 1% BSA. The cells were immunofluorescently labeled with the m1A antibody (left) diluted 1:1,000 in blocking solution followed by a goat anti-mouse antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with Hoechst33342. A merge of the two stainings is shown on the right.</small></p>
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<p><small><strong>Figure 3. Immunohistochemistry using the Diagenode monoclonal antibody directed against m1A</strong><br />Paraffin-embedded mouse liver tissue was analysed by immunohistochemical analysis using the Diagenode monoclonal antibody against m1A (cat. No. C15200235) diluted 1:10,000 (brown). The slides were counterstained with Hematoxylin (blue).</small></p>
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<td>15 &mu;g per IP</td>
<td>Fig 1</td>
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		'id' => '2968',
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		'name' => '1-methyladenosine Antibody',
		'description' => '<p><strong>Other names:</strong> m1A, 1mA, m1Ado</p>
<p>Monoclonal antibody raised in mouse against 1-methyladenosine (m1A) conjugated to KLH.</p>',
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<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15200235-ip-1.jpg" alt="RIP" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><small><strong>Figure 1. Immunoprecipitation using the Diagenode monoclonal antibody directed against m1A</strong><br />Immunoprecipitation was performed on 40 &micro;g total RNA isolated from HEK293 cells using 15 &micro;g of the Diagenode monoclonal antibody against m1A (cat. No. C15200235) or with an equal amount of mouse IgG2b, used as a negative control. The immunoprecipitated RNA was subsequently analysed on a Bioanalyzer. Figure 1 shows the Bioanalyzer profile obtained with the negative control (upper left) and the m1A antibody (upper right). The lower figure shows the gel image for the input, the negative IgG2b control and the m1A antibody (lane 1, 2 and 3 respectively). The marker (in bp) is shown on the left, the position of the 28s and 18s ribosomal RNA is indicated on the right.</small></p>
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<p><small><strong>Figure 2. Immunofluorescence using the Diagenode monoclonal antibody directed against m1A</strong><br />HeLa cells were stained with the Diagenode monoclonal antibody against m1A (cat. No. C15200235). Cells were fixed with 4% formaldehyde for 20 min at RT, permeabilized with 0.5% Triton X-100 for 5 min at RT and blocked with PBS containing 1% BSA. The cells were immunofluorescently labeled with the m1A antibody (left) diluted 1:1,000 in blocking solution followed by a goat anti-mouse antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with Hoechst33342. A merge of the two stainings is shown on the right.</small></p>
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<p><small><strong>Figure 3. Immunohistochemistry using the Diagenode monoclonal antibody directed against m1A</strong><br />Paraffin-embedded mouse liver tissue was analysed by immunohistochemical analysis using the Diagenode monoclonal antibody against m1A (cat. No. C15200235) diluted 1:10,000 (brown). The slides were counterstained with Hematoxylin (blue).</small></p>
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<td>IP</td>
<td>15 &mu;g per IP</td>
<td>Fig 1</td>
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<td>1:1,000</td>
<td>Fig 2</td>
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<p>Diagenode offers huge selection of highly sensitive antibodies validated in IF.</p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200229-IF.jpg" alt="" height="245" width="256" /></p>
<p><sup><strong>Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9</strong></sup></p>
<p><sup>HeLa cells transfected with a Cas9 expression vector (left) or untransfected cells (right) were fixed in methanol at -20&deg;C, permeabilized with acetone at -20&deg;C and blocked with PBS containing 2% BSA. The cells were stained with the Cas9 C-terminal antibody (Cat. No. C15200229) diluted 1:400, followed by incubation with an anti-mouse secondary antibody coupled to AF488. The bottom images show counter-staining of the nuclei with Hoechst 33342.</sup></p>
<h5><sup>Check our selection of antibodies validated in IF.</sup></h5>',
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			'meta_keywords' => 'immunohistochemistry,Monoclonal antibody,Polyclonal antibody',
			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for immunohistochemistry applications',
			'meta_title' => 'Immunohistochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode',
			'modified' => '2016-01-13 12:23:53',
			'created' => '2015-07-08 13:49:03',
			'ProductsApplication' => array(
				[maximum depth reached]
			)
		)
	),
	'Category' => array(
		(int) 0 => array(
			'id' => '103',
			'position' => '0',
			'parent_id' => '4',
			'name' => 'All antibodies',
			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
			'no_promo' => false,
			'in_menu' => true,
			'online' => true,
			'tabular' => false,
			'hide' => true,
			'all_format' => false,
			'is_antibody' => true,
			'slug' => 'all-antibodies',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
			'modified' => '2019-07-03 10:55:44',
			'created' => '2015-11-02 14:49:22',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		),
		(int) 1 => array(
			'id' => '109',
			'position' => '40',
			'parent_id' => '4',
			'name' => 'RNA modifications',
			'description' => '<div class="row">
<div style="text-align: justify;" class="large-12 columns">
<p><span style="font-weight: 400;">Diagenode&rsquo;s large range of highly validated antibodies includes the antibodies to study RNA modifications. These antibodies have been thoroughly validated for many applications (RIP, WB, IF, ELISA, Dot blot, IP). Check out all of our highly sensitive and specific antibodies for RNA modifications studies below.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>
</div>
</div>',
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			'is_antibody' => true,
			'slug' => 'rna-modifications-antibodies',
			'cookies_tag_id' => null,
			'meta_keywords' => 'm6A polyclonal antibody ,Monoclonal antibody',
			'meta_description' => 'Diagenode offers a wide range of  RNA modifications antibodies',
			'meta_title' => 'RNA modifications antibodies | Diagenode.com',
			'modified' => '2020-09-01 11:43:28',
			'created' => '2016-02-15 10:58:08',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		)
	),
	'Document' => array(
		(int) 0 => array(
			'id' => '1006',
			'name' => '1-methyladenosine monoclonal antibody',
			'description' => '<p>Monoclonal antibody raised in mouse against 1-methyladenosine (m1A) conjugated to KLH.</p>',
			'image_id' => null,
			'type' => 'Datasheet',
			'url' => 'files/products/antibodies/1-methyladenosine-monoclonal-antibody.pdf',
			'slug' => '1-methyladenosine-monoclonal-antibody-datasheet',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2018-05-09 15:38:51',
			'created' => '2018-05-09 15:38:51',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		)
	),
	'Feature' => array(),
	'Image' => array(),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array(
		(int) 0 => array(
			'id' => '1142',
			'name' => '1-methyladenosine antibody SDS GB en',
			'language' => 'en',
			'url' => 'files/SDS/1-methyladenosine/SDS-C15200235-1-methyladenosine_Antibody-GB-en-GHS_2_0.pdf',
			'countries' => 'GB',
			'modified' => '2021-01-26 16:24:21',
			'created' => '2021-01-26 16:24:21',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
			'id' => '1144',
			'name' => '1-methyladenosine antibody SDS US en',
			'language' => 'en',
			'url' => 'files/SDS/1-methyladenosine/SDS-C15200235-1-methyladenosine_Antibody-US-en-GHS_2_0.pdf',
			'countries' => 'US',
			'modified' => '2021-01-26 16:25:10',
			'created' => '2021-01-26 16:25:10',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '1139',
			'name' => '1-methyladenosine antibody SDS DE de',
			'language' => 'de',
			'url' => 'files/SDS/1-methyladenosine/SDS-C15200235-1-methyladenosine_Antibody-DE-de-GHS_2_0.pdf',
			'countries' => 'DE',
			'modified' => '2021-01-26 16:23:05',
			'created' => '2021-01-26 16:23:05',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '1143',
			'name' => '1-methyladenosine antibody SDS JP ja',
			'language' => 'ja',
			'url' => 'files/SDS/1-methyladenosine/SDS-C15200235-1-methyladenosine_Antibody-JP-ja-GHS_3_0.pdf',
			'countries' => 'JP',
			'modified' => '2021-01-26 16:24:47',
			'created' => '2021-01-26 16:24:47',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '1138',
			'name' => '1-methyladenosine antibody SDS BE nl',
			'language' => 'nl',
			'url' => 'files/SDS/1-methyladenosine/SDS-C15200235-1-methyladenosine_Antibody-BE-nl-GHS_2_0.pdf',
			'countries' => 'BE',
			'modified' => '2021-01-26 16:22:40',
			'created' => '2021-01-26 16:22:40',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
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		(int) 5 => array(
			'id' => '1137',
			'name' => '1-methyladenosine antibody SDS BE fr',
			'language' => 'fr',
			'url' => 'files/SDS/1-methyladenosine/SDS-C15200235-1-methyladenosine_Antibody-BE-fr-GHS_2_0.pdf',
			'countries' => 'BE',
			'modified' => '2021-01-26 16:22:15',
			'created' => '2021-01-26 16:22:15',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 6 => array(
			'id' => '1141',
			'name' => '1-methyladenosine antibody SDS FR fr',
			'language' => 'fr',
			'url' => 'files/SDS/1-methyladenosine/SDS-C15200235-1-methyladenosine_Antibody-FR-fr-GHS_2_0.pdf',
			'countries' => 'FR',
			'modified' => '2021-01-26 16:23:56',
			'created' => '2021-01-26 16:23:56',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 7 => array(
			'id' => '1140',
			'name' => '1-methyladenosine antibody SDS ES es',
			'language' => 'es',
			'url' => 'files/SDS/1-methyladenosine/SDS-C15200235-1-methyladenosine_Antibody-ES-es-GHS_2_0.pdf',
			'countries' => 'ES',
			'modified' => '2021-01-26 16:23:34',
			'created' => '2021-01-26 16:23:34',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		)
	)
)
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ''
$country_code = 'US'
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
	'id' => '32',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'IHC',
	'description' => '<p>Immunohistochemistry</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'immunohistochemistry',
	'meta_keywords' => 'immunohistochemistry,Monoclonal antibody,Polyclonal antibody',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for immunohistochemistry applications',
	'meta_title' => 'Immunohistochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode',
	'modified' => '2016-01-13 12:23:53',
	'created' => '2015-07-08 13:49:03',
	'ProductsApplication' => array(
		'id' => '4895',
		'product_id' => '2968',
		'application_id' => '32'
	)
)
$slugs = array(
	(int) 0 => 'immunohistochemistry'
)
$applications = array(
	'id' => '32',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'IHC',
	'description' => '<p>Immunohistochemistry</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'immunohistochemistry',
	'meta_keywords' => 'immunohistochemistry,Monoclonal antibody,Polyclonal antibody',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for immunohistochemistry applications',
	'meta_title' => 'Immunohistochemistry - Monoclonal antibody - Polyclonal antibody | Diagenode',
	'modified' => '2016-01-13 12:23:53',
	'created' => '2015-07-08 13:49:03',
	'locale' => 'eng'
)
$description = '<p>Immunohistochemistry</p>'
$name = 'IHC'
$document = array(
	'id' => '1006',
	'name' => '1-methyladenosine monoclonal antibody',
	'description' => '<p>Monoclonal antibody raised in mouse against 1-methyladenosine (m1A) conjugated to KLH.</p>',
	'image_id' => null,
	'type' => 'Datasheet',
	'url' => 'files/products/antibodies/1-methyladenosine-monoclonal-antibody.pdf',
	'slug' => '1-methyladenosine-monoclonal-antibody-datasheet',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2018-05-09 15:38:51',
	'created' => '2018-05-09 15:38:51',
	'ProductsDocument' => array(
		'id' => '2618',
		'product_id' => '2968',
		'document_id' => '1006'
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)
$sds = array(
	'id' => '1140',
	'name' => '1-methyladenosine antibody SDS ES es',
	'language' => 'es',
	'url' => 'files/SDS/1-methyladenosine/SDS-C15200235-1-methyladenosine_Antibody-ES-es-GHS_2_0.pdf',
	'countries' => 'ES',
	'modified' => '2021-01-26 16:23:34',
	'created' => '2021-01-26 16:23:34',
	'ProductsSafetySheet' => array(
		'id' => '2010',
		'product_id' => '2968',
		'safety_sheet_id' => '1140'
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)
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118