6X His epitope tag monoclonal antibody

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<p><small><strong> Figure 1. 6X His Tag antibody ELISA results </strong><br />ELISA using the monoclonal 6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and FLAG-tagged purified proteins. Coating amount: 0.15ug per welll. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. </small></p>
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<p><small><strong> Figure 2. 6X His Tag antibody western blot results </strong><br />Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody used at a 1:5,000 dilution overnight at 4&deg;C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. </small></p>
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<p><small><strong> Figure 3. 6X His Tag antibody western blot results </strong><br />Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). A molecular weight marker is shown in lane 1. </small></p>
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<p><small><strong> Figure 1. 6X His Tag antibody ELISA results </strong><br />ELISA using the monoclonal 6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and FLAG-tagged purified proteins. Coating amount: 0.15ug per welll. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. </small></p>
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<p><small><strong> Figure 2. 6X His Tag antibody western blot results </strong><br />Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody used at a 1:5,000 dilution overnight at 4&deg;C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. </small></p>
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<p><small><strong> Figure 3. 6X His Tag antibody western blot results </strong><br />Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). A molecular weight marker is shown in lane 1. </small></p>
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<td>1:75,000</td>
<td></td>
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<td>1:1,000</td>
<td></td>
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<td>Fig 1, 2</td>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]
Code Context
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<p><small><strong> Figure 1. 6X His Tag antibody ELISA results </strong><br />ELISA using the monoclonal 6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and FLAG-tagged purified proteins. Coating amount: 0.15ug per welll. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. </small></p>
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<p><small><strong> Figure 2. 6X His Tag antibody western blot results </strong><br />Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody used at a 1:5,000 dilution overnight at 4&deg;C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. </small></p>
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<p><small><strong> Figure 2. 6X His Tag antibody western blot results </strong><br />Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody used at a 1:5,000 dilution overnight at 4&deg;C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. </small></p>
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<p><small><strong> Figure 3. 6X His Tag antibody western blot results </strong><br />Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). A molecular weight marker is shown in lane 1. </small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]
Code Context
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<p><small><strong> Figure 1. 6X His Tag antibody ELISA results </strong><br />ELISA using the monoclonal 6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and FLAG-tagged purified proteins. Coating amount: 0.15ug per welll. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. </small></p>
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<p><small><strong> Figure 2. 6X His Tag antibody western blot results </strong><br />Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody used at a 1:5,000 dilution overnight at 4&deg;C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. </small></p>
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<p><small><strong> Figure 3. 6X His Tag antibody western blot results </strong><br />Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). A molecular weight marker is shown in lane 1. </small></p>
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<p><small><strong> Figure 1. 6X His Tag antibody ELISA results </strong><br />ELISA using the monoclonal 6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and FLAG-tagged purified proteins. Coating amount: 0.15ug per welll. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200208-WB.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="176" height="242" /></p>
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<p><small><strong> Figure 2. 6X His Tag antibody western blot results </strong><br />Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody used at a 1:5,000 dilution overnight at 4&deg;C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200208-6X-WB-2.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="152" height="215" /></p>
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<p><small><strong> Figure 3. 6X His Tag antibody western blot results </strong><br />Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). A molecular weight marker is shown in lane 1. </small></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Cost-effective (requires less antibody per reaction)</li>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]
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<p><small><strong> Figure 1. 6X His Tag antibody ELISA results </strong><br />ELISA using the monoclonal 6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and FLAG-tagged purified proteins. Coating amount: 0.15ug per welll. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. </small></p>
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<p><small><strong> Figure 2. 6X His Tag antibody western blot results </strong><br />Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody used at a 1:5,000 dilution overnight at 4&deg;C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. </small></p>
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<p><small><strong> Figure 3. 6X His Tag antibody western blot results </strong><br />Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). A molecular weight marker is shown in lane 1. </small></p>
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			'created' => '2024-01-17 15:58:05',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 6 => array(
			'id' => '3562',
			'name' => 'SDS C15200208 6X His epitope tag Antibody FR fr',
			'language' => 'fr',
			'url' => 'files/SDS/6x_his/SDS-C15200208-6X_His_epitope_tag_Antibody-FR-fr-GHS_1_0.pdf',
			'countries' => 'FR',
			'modified' => '2024-01-17 15:56:44',
			'created' => '2024-01-17 15:56:44',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 7 => array(
			'id' => '3569',
			'name' => 'SDS C15200208 6X His epitope tag Antibody ES es',
			'language' => 'es',
			'url' => 'files/SDS/6x_his/SDS-C15200208-6X_His_epitope_tag_Antibody-ES-es-GHS_1_0.pdf',
			'countries' => 'ES',
			'modified' => '2024-01-17 15:59:11',
			'created' => '2024-01-17 15:59:11',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		)
	)
)
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ''
$country_code = 'US'
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
	'id' => '30',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'IP',
	'description' => '<p>Immunoprecipitation</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'immunoprecipitation',
	'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications',
	'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode',
	'modified' => '2016-01-13 12:23:07',
	'created' => '2015-07-08 13:46:50',
	'ProductsApplication' => array(
		'id' => '1937',
		'product_id' => '2018',
		'application_id' => '30'
	)
)
$slugs = array(
	(int) 0 => 'immunoprecipitation'
)
$applications = array(
	'id' => '30',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'IP',
	'description' => '<p>Immunoprecipitation</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'immunoprecipitation',
	'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications',
	'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode',
	'modified' => '2016-01-13 12:23:07',
	'created' => '2015-07-08 13:46:50',
	'locale' => 'jpn'
)
$description = '<p>Immunoprecipitation</p>'
$name = 'IP'
$document = array(
	'id' => '489',
	'name' => 'Datasheet 6X His epitope tag C15200208',
	'description' => 'Datasheet description',
	'image_id' => null,
	'type' => 'Datasheet',
	'url' => 'files/products/antibodies/Datasheet_6X_His_epitope_tag_C15200208.pdf',
	'slug' => 'datasheet-6x-his-epitope-tag-c15200208',
	'meta_keywords' => null,
	'meta_description' => null,
	'modified' => '2015-07-07 11:47:44',
	'created' => '2015-07-07 11:47:44',
	'ProductsDocument' => array(
		'id' => '141',
		'product_id' => '2018',
		'document_id' => '489'
	)
)
$sds = array(
	'id' => '3569',
	'name' => 'SDS C15200208 6X His epitope tag Antibody ES es',
	'language' => 'es',
	'url' => 'files/SDS/6x_his/SDS-C15200208-6X_His_epitope_tag_Antibody-ES-es-GHS_1_0.pdf',
	'countries' => 'ES',
	'modified' => '2024-01-17 15:59:11',
	'created' => '2024-01-17 15:59:11',
	'ProductsSafetySheet' => array(
		'id' => '5816',
		'product_id' => '2018',
		'safety_sheet_id' => '3569'
	)
)
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118