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<p><small><strong> Figure 1. 6X His Tag antibody ELISA results </strong><br />ELISA using the monoclonal 6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and FLAG-tagged purified proteins. Coating amount: 0.15ug per welll. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. </small></p>
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<p><small><strong> Figure 2. 6X His Tag antibody western blot results </strong><br />Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody used at a 1:5,000 dilution overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. </small></p>
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<p><small><strong> Figure 3. 6X His Tag antibody western blot results </strong><br />Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). A molecular weight marker is shown in lane 1. </small></p>
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<p><small><strong> Figure 1. 6X His Tag antibody ELISA results </strong><br />ELISA using the monoclonal 6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and FLAG-tagged purified proteins. Coating amount: 0.15ug per welll. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. </small></p>
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<p><small><strong> Figure 2. 6X His Tag antibody western blot results </strong><br />Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody used at a 1:5,000 dilution overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. </small></p>
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<p><small><strong> Figure 3. 6X His Tag antibody western blot results </strong><br />Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). A molecular weight marker is shown in lane 1. </small></p>
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<p><small><strong> Figure 3. 6X His Tag antibody western blot results </strong><br />Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). A molecular weight marker is shown in lane 1. </small></p>
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<p><small><strong> Figure 3. 6X His Tag antibody western blot results </strong><br />Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). A molecular weight marker is shown in lane 1. </small></p>
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<p><small><strong> Figure 2. 6X His Tag antibody western blot results </strong><br />Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody used at a 1:5,000 dilution overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. </small></p>
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<p><small><strong> Figure 3. 6X His Tag antibody western blot results </strong><br />Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). A molecular weight marker is shown in lane 1. </small></p>
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<p><small><strong> Figure 2. 6X His Tag antibody western blot results </strong><br />Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody used at a 1:5,000 dilution overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. </small></p>
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<p><small><strong> Figure 3. 6X His Tag antibody western blot results </strong><br />Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). A molecular weight marker is shown in lane 1. </small></p>
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<td>ELISA</td>
<td>1:75,000</td>
<td></td>
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<td>Western Blotting</td>
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<td></td>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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<li>100% satisfaction guarantee</li>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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