6X His epitope tag Antibody

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

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Antibodies

Catalog Number

Format

Price

C15200208

100 µg

$440.00
Bulk order

Monoclonal antibody raised in mouse against 6X His epitope tag, using a KLH-conjugated synthetic peptide.

Lot	001
Concentration	1.0 μg/μl
Species reactivity
Type	Monoclonal
Purity	Affinity purified
Host	Mouse
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ELISA	1:75,000
Western Blotting	1:1,000
Immunoprecipitation	1:200	Fig 1, 2

Validation Data

Figure 1. 6X His Tag antibody ELISA results
ELISA using the monoclonal 6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and FLAG-tagged purified proteins. Coating amount: 0.15ug per welll. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000.

Figure 2. 6X His Tag antibody western blot results
Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody used at a 1:5,000 dilution overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT.

Figure 3. 6X His Tag antibody western blot results
Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). A molecular weight marker is shown in lane 1.

Documents
Datasheet 6X His epitope tag C15200208 DATASHEET
Datasheet description Download

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<p><small><strong> Figure 1. 6X His Tag antibody ELISA results </strong><br />ELISA using the monoclonal 6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and FLAG-tagged purified proteins. Coating amount: 0.15ug per welll. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. </small></p>
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<p><small><strong> Figure 2. 6X His Tag antibody western blot results </strong><br />Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody used at a 1:5,000 dilution overnight at 4&deg;C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. </small></p>
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<p><small><strong> Figure 3. 6X His Tag antibody western blot results </strong><br />Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). A molecular weight marker is shown in lane 1. </small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong> Figure 3. 6X His Tag antibody western blot results </strong><br />Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). A molecular weight marker is shown in lane 1. </small></p>
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<p><small><strong> Figure 3. 6X His Tag antibody western blot results </strong><br />Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). A molecular weight marker is shown in lane 1. </small></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Cost-effective (requires less antibody per reaction)</li>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
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<p><small><strong> Figure 1. 6X His Tag antibody ELISA results </strong><br />ELISA using the monoclonal 6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and FLAG-tagged purified proteins. Coating amount: 0.15ug per welll. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. </small></p>
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</div>
<div class="row">
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<div class="small-9 columns">
<p><small><strong> Figure 2. 6X His Tag antibody western blot results </strong><br />Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody used at a 1:5,000 dilution overnight at 4&deg;C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. </small></p>
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</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15200208-6X-WB-2.png" alt="Western Blot" style="display: block; margin-left: auto; margin-right: auto;" width="152" height="215" /></p>
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<div class="small-9 columns">
<p><small><strong> Figure 3. 6X His Tag antibody western blot results </strong><br />Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). A molecular weight marker is shown in lane 1. </small></p>
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			'description' => '6X His Tag Antibody as well as other Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.',
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			'isotype' => '',
			'lot' => '001',
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<thead>
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<td></td>
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<td>Fig 1, 2</td>
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<p><small><strong> Figure 1. 6X His Tag antibody ELISA results </strong><br />ELISA using the monoclonal 6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and FLAG-tagged purified proteins. Coating amount: 0.15ug per welll. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. </small></p>
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<p><small><strong> Figure 2. 6X His Tag antibody western blot results </strong><br />Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody used at a 1:5,000 dilution overnight at 4&deg;C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. </small></p>
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<p><small><strong> Figure 3. 6X His Tag antibody western blot results </strong><br />Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). A molecular weight marker is shown in lane 1. </small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong> Figure 1. 6X His Tag antibody ELISA results </strong><br />ELISA using the monoclonal 6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and FLAG-tagged purified proteins. Coating amount: 0.15ug per welll. Primary antibody: 6xHIS Tag antibody at 100ug/mL. Dilution series: 2-fold. Mid-point concentration: 200ng/mL. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000. </small></p>
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<p><small><strong> Figure 2. 6X His Tag antibody western blot results </strong><br />Western Blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody used at a 1:5,000 dilution overnight at 4&deg;C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20,000 for 30 min at RT. </small></p>
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<p><small><strong> Figure 3. 6X His Tag antibody western blot results </strong><br />Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). A molecular weight marker is shown in lane 1. </small></p>
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<p><small><strong> Figure 3. 6X His Tag antibody western blot results </strong><br />Western Blot using the monoclonal 6x-His Epitope Tag Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). A molecular weight marker is shown in lane 1. </small></p>
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			'url' => 'files/SDS/6x_his/SDS-C15200208-6X_His_epitope_tag_Antibody-DE-de-GHS_1_0.pdf',
			'countries' => 'DE',
			'modified' => '2024-01-17 15:58:41',
			'created' => '2024-01-17 15:58:41',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '3564',
			'name' => 'SDS C15200208 6X His epitope tag Antibody JP ja',
			'language' => 'ja',
			'url' => 'files/SDS/6x_his/SDS-C15200208-6X_His_epitope_tag_Antibody-JP-ja-GHS_1_0.pdf',
			'countries' => 'JP',
			'modified' => '2024-01-17 15:57:29',
			'created' => '2024-01-17 15:57:29',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '3567',
			'name' => 'SDS C15200208 6X His epitope tag Antibody BE nl',
			'language' => 'nl',
			'url' => 'files/SDS/6x_his/SDS-C15200208-6X_His_epitope_tag_Antibody-BE-nl-GHS_1_0.pdf',
			'countries' => 'BE',
			'modified' => '2024-01-17 15:58:24',
			'created' => '2024-01-17 15:58:24',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 5 => array(
			'id' => '3566',
			'name' => 'SDS C15200208 6X His epitope tag Antibody BE fr',
			'language' => 'fr',
			'url' => 'files/SDS/6x_his/SDS-C15200208-6X_His_epitope_tag_Antibody-BE-fr-GHS_1_0.pdf',
			'countries' => 'BE',
			'modified' => '2024-01-17 15:58:05',
			'created' => '2024-01-17 15:58:05',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 6 => array(
			'id' => '3562',
			'name' => 'SDS C15200208 6X His epitope tag Antibody FR fr',
			'language' => 'fr',
			'url' => 'files/SDS/6x_his/SDS-C15200208-6X_His_epitope_tag_Antibody-FR-fr-GHS_1_0.pdf',
			'countries' => 'FR',
			'modified' => '2024-01-17 15:56:44',
			'created' => '2024-01-17 15:56:44',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 7 => array(
			'id' => '3569',
			'name' => 'SDS C15200208 6X His epitope tag Antibody ES es',
			'language' => 'es',
			'url' => 'files/SDS/6x_his/SDS-C15200208-6X_His_epitope_tag_Antibody-ES-es-GHS_1_0.pdf',
			'countries' => 'ES',
			'modified' => '2024-01-17 15:59:11',
			'created' => '2024-01-17 15:59:11',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		)
	)
)
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ''
$country_code = 'US'
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
	'id' => '30',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'IP',
	'description' => '<p>Immunoprecipitation</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'immunoprecipitation',
	'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications',
	'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode',
	'modified' => '2016-01-13 12:23:07',
	'created' => '2015-07-08 13:46:50',
	'ProductsApplication' => array(
		'id' => '1937',
		'product_id' => '2018',
		'application_id' => '30'
	)
)
$slugs = array(
	(int) 0 => 'immunoprecipitation'
)
$applications = array(
	'id' => '30',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'IP',
	'description' => '<p>Immunoprecipitation</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'immunoprecipitation',
	'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications',
	'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode',
	'modified' => '2016-01-13 12:23:07',
	'created' => '2015-07-08 13:46:50',
	'locale' => 'eng'
)
$description = '<p>Immunoprecipitation</p>'
$name = 'IP'
$document = array(
	'id' => '489',
	'name' => 'Datasheet 6X His epitope tag C15200208',
	'description' => 'Datasheet description',
	'image_id' => null,
	'type' => 'Datasheet',
	'url' => 'files/products/antibodies/Datasheet_6X_His_epitope_tag_C15200208.pdf',
	'slug' => 'datasheet-6x-his-epitope-tag-c15200208',
	'meta_keywords' => null,
	'meta_description' => null,
	'modified' => '2015-07-07 11:47:44',
	'created' => '2015-07-07 11:47:44',
	'ProductsDocument' => array(
		'id' => '141',
		'product_id' => '2018',
		'document_id' => '489'
	)
)
$sds = array(
	'id' => '3569',
	'name' => 'SDS C15200208 6X His epitope tag Antibody ES es',
	'language' => 'es',
	'url' => 'files/SDS/6x_his/SDS-C15200208-6X_His_epitope_tag_Antibody-ES-es-GHS_1_0.pdf',
	'countries' => 'ES',
	'modified' => '2024-01-17 15:59:11',
	'created' => '2024-01-17 15:59:11',
	'ProductsSafetySheet' => array(
		'id' => '5816',
		'product_id' => '2018',
		'safety_sheet_id' => '3569'
	)
)
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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