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Acidaminococcus sp. CRISPR/Cpf1 monoclonal antibody

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Catalog Number

Format

Price

C15200234-10

10 µg

$130.00
Bulk order

Other format

C15200234-100

100 µg

C15200234-50

50 µg/50 µl

Monoclonal antibody raised in mouse against Acidaminococcus sp. (As) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.

Lot	001
Concentration	1 µg/µl
Species reactivity	Acidaminococcus sp.
Type	Monoclonal
Purity	Protein G purified monoclonal antibody
Host	Mouse
Storage Conditions	Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage Buffer	TBS containing 0.01 % Na-azide
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
Western blotting	1:1,000	Fig 1

Validation data

Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against AsCRISPR/Cpf1
Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 1, 3) or HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 2, 4) using the Diagenode monoclonal antibody against AsCRISPR/Cpf1 (cat. No. C15200234), diluted 1:1,000 in PBS-T containing 0.5% NFDM (lane 1 and 2). Lanes 3 and 4 show the WB result with an antibody against the HA-tag.

資料
Acidaminococcus sp. CRISPR/Cpf1 monoclonal antibody DATASHEET
Monoclonal antibody raised in mouse against Acidaminococcus sp. (As) Cpf1 (CRISPR from Prevotella... Download

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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 1, 3) or HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 2, 4) using the Diagenode monoclonal antibody against AsCRISPR/Cpf1 (cat. No. C15200234), diluted 1:1,000 in PBS-T containing 0.5% NFDM (lane 1 and 2). Lanes 3 and 4 show the WB result with an antibody against the HA-tag.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
<ul>
<li>Raised against the Cpf1 nuclease from <em>Acidaminococcus sp.</em></li>
<li>Ideal for Western blot</li>
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<div class="row">
<div class="small-3 medium-3 large-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/cat-cpf1-fig1.png" /></div>
<div class="small-9 medium-9 large-9 columns">
<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<li>Raised against the Cpf1 nuclease from <em>Lachnospiraceae bacterium</em></li>
<li>Validated in Western blot, <g class="gr_ gr_22 gr-alert gr_gramm gr_disable_anim_appear Punctuation only-ins replaceWithoutSep" id="22" data-gr-id="22">immunoprecipitation</g> and immunofluorescence</li>
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<p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4&deg;C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
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<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong>&nbsp;with rigorous QC and validation</li>
<li><strong>Classified</strong>&nbsp;based on level of validation for flexibility of application</li>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4&deg;C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 1, 3) or HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 2, 4) using the Diagenode monoclonal antibody against AsCRISPR/Cpf1 (cat. No. C15200234), diluted 1:1,000 in PBS-T containing 0.5% NFDM (lane 1 and 2). Lanes 3 and 4 show the WB result with an antibody against the HA-tag.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
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			'created' => '2018-02-22 15:59:49',
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			'description' => 'CRISPR systems are adaptable immune mechanisms which are present in many bacteria to protect themselves from foreign nucleic acids, such as viruses, transposable elements or plasmids. The CRISPR/Cas9 (CRISPR-associated protein 9nuclease) system from S. pyogenes was the first to be adapted for inducing sequence-specific double stranded breaks and targeted genome editing. This system is unique and flexible due to its dependence on RNA as the moiety that targets the nuclease to a desired DNA sequence and can be used to induce indel mutations, specific sequence replacements or insertions and large deletions or genomic rearrangements at any desired location in the genome. In addition, Cas9 can also be used to mediate upregulation of specific endogenous genes or to alter histone modifications or DNA methylation. Recently, a so-called type V CRISPR system has been identified in several bacteria which contains the Cpf1 (CRISPR from Prevotella and Francisella 1) protein. In contrast to Cas9 systems, CRISPR/Cpf1 systems do not require an additional trans-activating crRNA (tracrRNA), they cleave target DNA proceeded by a short T-rich protospacer-adjacent motif (PAM), in contrast to the G-rich PAM following the target DNA for Cas9, and they introduce a staggered DNA doublestranded break with a 4 or 5-nt 5’ overhang. Two of these CRISPR/Cpf1 systems, present in <em>Acidaminococcus sp.</em> and Lachnospiraceae bacterium have been identified as potential candidates for genome editing in mammalian cells.',
			'clonality' => '',
			'isotype' => 'IgG2a',
			'lot' => '001',
			'concentration' => '1 µg/µl',
			'reactivity' => '<em>Acidaminococcus sp.</em>',
			'type' => 'Monoclonal',
			'purity' => 'Protein G purified monoclonal antibody',
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			'application_table' => '<table>
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<td>Fig 1</td>
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			'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 1, 3) or HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 2, 4) using the Diagenode monoclonal antibody against AsCRISPR/Cpf1 (cat. No. C15200234), diluted 1:1,000 in PBS-T containing 0.5% NFDM (lane 1 and 2). Lanes 3 and 4 show the WB result with an antibody against the HA-tag.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 1, 3) or HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 2, 4) using the Diagenode monoclonal antibody against AsCRISPR/Cpf1 (cat. No. C15200234), diluted 1:1,000 in PBS-T containing 0.5% NFDM (lane 1 and 2). Lanes 3 and 4 show the WB result with an antibody against the HA-tag.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4&deg;C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 1, 3) or HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 2, 4) using the Diagenode monoclonal antibody against AsCRISPR/Cpf1 (cat. No. C15200234), diluted 1:1,000 in PBS-T containing 0.5% NFDM (lane 1 and 2). Lanes 3 and 4 show the WB result with an antibody against the HA-tag.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 1, 3) or HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 2, 4) using the Diagenode monoclonal antibody against AsCRISPR/Cpf1 (cat. No. C15200234), diluted 1:1,000 in PBS-T containing 0.5% NFDM (lane 1 and 2). Lanes 3 and 4 show the WB result with an antibody against the HA-tag.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 1, 3) or HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 2, 4) using the Diagenode monoclonal antibody against AsCRISPR/Cpf1 (cat. No. C15200234), diluted 1:1,000 in PBS-T containing 0.5% NFDM (lane 1 and 2). Lanes 3 and 4 show the WB result with an antibody against the HA-tag.</small></p>
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<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4&deg;C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 1, 3) or HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 2, 4) using the Diagenode monoclonal antibody against AsCRISPR/Cpf1 (cat. No. C15200234), diluted 1:1,000 in PBS-T containing 0.5% NFDM (lane 1 and 2). Lanes 3 and 4 show the WB result with an antibody against the HA-tag.</small></p>
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