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Acidaminococcus sp. CRISPR/Cpf1 monoclonal antibody

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Catalog Number

Format

Price

C15200234-50

50 µg/50 µl

$450.00
Bulk order

Other format

C15200234-100

100 µg

C15200234-10

10 µg

Monoclonal antibody raised in mouse against Acidaminococcus sp. (As) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein.

Lot	001
Concentration	1 µg/µl
Species reactivity	Acidaminococcus sp.
Type	Monoclonal
Purity	Protein G purified monoclonal antibody
Host	Mouse
Storage Conditions	Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage Buffer	TBS containing 0.01 % Na-azide
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
Western blotting	1:1,000	Fig 1

Validation data

Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against AsCRISPR/Cpf1
Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 1, 3) or HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 2, 4) using the Diagenode monoclonal antibody against AsCRISPR/Cpf1 (cat. No. C15200234), diluted 1:1,000 in PBS-T containing 0.5% NFDM (lane 1 and 2). Lanes 3 and 4 show the WB result with an antibody against the HA-tag.

資料
Acidaminococcus sp. CRISPR/Cpf1 monoclonal antibody DATASHEET
Monoclonal antibody raised in mouse against Acidaminococcus sp. (As) Cpf1 (CRISPR from Prevotella... Download

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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 1, 3) or HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 2, 4) using the Diagenode monoclonal antibody against AsCRISPR/Cpf1 (cat. No. C15200234), diluted 1:1,000 in PBS-T containing 0.5% NFDM (lane 1 and 2). Lanes 3 and 4 show the WB result with an antibody against the HA-tag.</small></p>
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<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
<ul>
<li>Raised against the Cpf1 nuclease from <em>Acidaminococcus sp.</em></li>
<li>Ideal for Western blot</li>
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<div class="row">
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<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
<ul>
<li>Raised against the Cpf1 nuclease from <em>Lachnospiraceae bacterium</em></li>
<li>Validated in Western blot, <g class="gr_ gr_22 gr-alert gr_gramm gr_disable_anim_appear Punctuation only-ins replaceWithoutSep" id="22" data-gr-id="22">immunoprecipitation</g> and immunofluorescence</li>
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<p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4&deg;C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 1, 3) or HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 2, 4) using the Diagenode monoclonal antibody against AsCRISPR/Cpf1 (cat. No. C15200234), diluted 1:1,000 in PBS-T containing 0.5% NFDM (lane 1 and 2). Lanes 3 and 4 show the WB result with an antibody against the HA-tag.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
$name = 'WB'
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Transiently transfected HEK293 cells expressing HA-tagged LbCRISPR/Cpf1 were fixed with 3.7% formaldehyde, permeabilized in 0.5% Triton-X-100 and blocked in PBS containing 2% BSA for 2 hours at RT. The cells were stained with the LbCRISPR/Cpf1 antibody (Cat. No. C15310263) diluted 1:500 in blocking solution at 4&deg;C o/n followed by incubation with an anti-rabbit secondary antibody coupled to DyLight594 for 1 h at RT (left figure). Nuclei were counter-stained with Hoechst 33342 (right). The middle figure shows IF with an anti-HA antibody.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 1, 3) or HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 2, 4) using the Diagenode monoclonal antibody against AsCRISPR/Cpf1 (cat. No. C15200234), diluted 1:1,000 in PBS-T containing 0.5% NFDM (lane 1 and 2). Lanes 3 and 4 show the WB result with an antibody against the HA-tag.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 1, 3) or HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 2, 4) using the Diagenode monoclonal antibody against AsCRISPR/Cpf1 (cat. No. C15200234), diluted 1:1,000 in PBS-T containing 0.5% NFDM (lane 1 and 2). Lanes 3 and 4 show the WB result with an antibody against the HA-tag.</small></p>
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<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p><small>Western blot was performed on protein extracts from HEK293 cells using the Diagenode antibody against AsCRISPR/Cpf1 (C15310262), diluted 1:5,000 in PBS-T containing 3% NFDM. The marker is shown on the left, the position of the Cpf1 protein is indicated on the right. Lane 1 shows the Western blot analysis with the pre-immune serum, used as a negative control.</small></p>
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<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
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$applications = array(
	'id' => '19',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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	'slug' => 'western-blot-antibodies',
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	'created' => '2015-01-07 09:20:00',
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
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$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
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<th>Suggested dilution</th>
<th>References</th>
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<tbody>
<tr>
<td>Western blotting</td>
<td>1:1,000</td>
<td>Fig 1</td>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against AsCRISPR/Cpf1</strong><br />Western blot was performed on protein extracts from HEK293 cells transfected with HA-tagged AsCRISPR/Cpf1 (lane 1, 3) or HEK293 cells transfected with HA-tagged LbCRISPR/Cpf1 (lane 2, 4) using the Diagenode monoclonal antibody against AsCRISPR/Cpf1 (cat. No. C15200234), diluted 1:1,000 in PBS-T containing 0.5% NFDM (lane 1 and 2). Lanes 3 and 4 show the WB result with an antibody against the HA-tag.</small></p>
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<h2><a href="../p/acidaminococcus-sp-crispr-cpf1-polyclonal-antibody"><em>Acidaminococcus sp.</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<h2><a href="../p/l-bacteriumcrispr-cpf1-polyclonal-antibody"><em>L. bacterium</em> CRISPR/Cpf1 polyclonal antibody</a></h2>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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