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AF9 polyclonal antibody

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

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Antibodies

Catalog Number

Format

Price

C15310266-20

20 µl

$115.00
Bulk order

Other format

C15310266

100 μl

Other names: MLLT3, YEATS3

Polyclonal antibody raised in rabbit against human AF9 (Super Elongation Complex Subunit) using a KLH conjugated synthetic peptide containing a sequence from the central region of the protein.

Lot	A1474-001
Concentration	Not determined
Species reactivity	Human: positive. Other species: not tested.
Type	Polyclonal ChIP grade, ChIP-seq grade
Purity	Whole antiserum from rabbit containing 0.05% azide.
Host	Rabbit
Storage Conditions	Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution/amount	References
ChIP/ChIP-seq *	2 µl/ChIP	Fig 1, 2
ELISA	1:100 - 1:500	Fig 3

*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µl per IP.

Validation Data

Figure 1. ChIP results obtained with the Diagenode antibody directed against AF9
ChIP was performed on THP-1 cells using the Diagenode antibody against AF9 (Cat. No. C15310266). Sheared chromatin from 1 million cells and 2 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).

Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AF9
ChIP was performed as described above. The IP’d DNA of 5 ChIP’s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 400 kb region of chromosome 6 containing the PHIP positive control gene (fig 2A and B), and in 3 genomic regions surrounding the CDKN2C gene, the JMJD1C gene and HOX cluster on chromosome 1, 10 and 7, respectively (fig 2C, D and E).

Figure 3. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.
Target Description
AF9 (UniProtKB/Swiss-Prot entry P42568) is a component of the super elongation complex which is required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.
実験手法
ELISA
Enzyme-linked immunosorbent assay. Read more
ChIP-seq (ab)
Read more
ChIP-qPCR (ab)
Read more
資料
AF9 polyclonal antibody DATASHEET
Polyclonal antibody raised in rabbit against human AF9 (Super Elongation Complex Subunit) using a... Download

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<p>Polyclonal antibody raised in rabbit against human <strong>AF9</strong> <strong>(Super Elongation Complex Subunit)</strong> using a KLH conjugated synthetic peptide containing a sequence from the central region of the protein.</p>',
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed on THP-1 cells using the Diagenode antibody against AF9 (Cat. No. C15310266). Sheared chromatin from 1 million cells and 2 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 5 ChIP&rsquo;s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 400 kb region of chromosome 6 containing the PHIP positive control gene (fig 2A and B), and in 3 genomic regions surrounding the CDKN2C gene, the JMJD1C gene and HOX cluster on chromosome 1, 10 and 7, respectively (fig 2C, D and E).</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.</small></p>
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<td>Fig 1, 2</td>
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<p>Polyclonal antibody raised in rabbit against human <strong>AF9</strong> <strong>(Super Elongation Complex Subunit)</strong> using a KLH conjugated synthetic peptide containing a sequence from the central region of the protein.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-b.png" alt="AF9 Antibody for ChIP-seq" /></p>
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<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-elisa.png" alt="AF9 Antibody ELISA Validation" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.</small></p>
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			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
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<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
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<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
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<p>Existing sample sizes are listed below. We will soon expand our collection. &nbsp;Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
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<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode&rsquo;s highly validated antibodies:</p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Highly sensitive and specific</span></li>
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		'description' => '<p><strong>Other names:</strong> MLLT3, YEATS3</p>
<p>Polyclonal antibody raised in rabbit against human <strong>AF9</strong> <strong>(Super Elongation Complex Subunit)</strong> using a KLH conjugated synthetic peptide containing a sequence from the central region of the protein.</p>',
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<div class="small-6 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed on THP-1 cells using the Diagenode antibody against AF9 (Cat. No. C15310266). Sheared chromatin from 1 million cells and 2 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).</small></p>
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<div class="small-12 columns">
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 5 ChIP&rsquo;s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 400 kb region of chromosome 6 containing the PHIP positive control gene (fig 2A and B), and in 3 genomic regions surrounding the CDKN2C gene, the JMJD1C gene and HOX cluster on chromosome 1, 10 and 7, respectively (fig 2C, D and E).</small></p>
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<div class="small-6 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.</small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed on THP-1 cells using the Diagenode antibody against AF9 (Cat. No. C15310266). Sheared chromatin from 1 million cells and 2 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 5 ChIP&rsquo;s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 400 kb region of chromosome 6 containing the PHIP positive control gene (fig 2A and B), and in 3 genomic regions surrounding the CDKN2C gene, the JMJD1C gene and HOX cluster on chromosome 1, 10 and 7, respectively (fig 2C, D and E).</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 5 ChIP&rsquo;s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 400 kb region of chromosome 6 containing the PHIP positive control gene (fig 2A and B), and in 3 genomic regions surrounding the CDKN2C gene, the JMJD1C gene and HOX cluster on chromosome 1, 10 and 7, respectively (fig 2C, D and E).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-elisa.png" alt="AF9 Antibody ELISA Validation" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.</small></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
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<li>Batch-specific data is available on the website</li>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode&rsquo;s highly validated antibodies:</p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<p>Polyclonal antibody raised in rabbit against human <strong>AF9</strong> <strong>(Super Elongation Complex Subunit)</strong> using a KLH conjugated synthetic peptide containing a sequence from the central region of the protein.</p>',
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed on THP-1 cells using the Diagenode antibody against AF9 (Cat. No. C15310266). Sheared chromatin from 1 million cells and 2 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 5 ChIP&rsquo;s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 400 kb region of chromosome 6 containing the PHIP positive control gene (fig 2A and B), and in 3 genomic regions surrounding the CDKN2C gene, the JMJD1C gene and HOX cluster on chromosome 1, 10 and 7, respectively (fig 2C, D and E).</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.</small></p>
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View::render() - CORE/Cake/View/View.php, line 473
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.</small></p>
</div>
</div>',
		'label2' => 'Target Description',
		'info2' => '<p>AF9 (UniProtKB/Swiss-Prot entry P42568) is a component of the super elongation complex which is required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.</p>',
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		'format' => '20 µl',
		'catalog_number' => 'C15310266-20',
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		'slug' => 'af9-polyclonal-antibody-20-ul',
		'meta_title' => '',
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		'modified' => '2024-01-16 13:13:18',
		'created' => '2017-10-12 16:10:59',
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	'Antibody' => array(
		'host' => '*****',
		'id' => '508',
		'name' => 'AF9 polyclonal antibody',
		'description' => 'AF9 (UniProtKB/Swiss-Prot entry P42568) is a component of the super elongation complex which is required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.',
		'clonality' => '',
		'isotype' => '',
		'lot' => 'A1474-001',
		'concentration' => 'Not determined',
		'reactivity' => 'Human: positive. Other species: not tested.',
		'type' => 'Polyclonal <strong>ChIP grade, ChIP-seq grade</strong>',
		'purity' => 'Whole antiserum from rabbit containing 0.05% azide.',
		'classification' => 'Classic',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution/amount</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq *</td>
<td>2 &micro;l/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:100 - 1:500</td>
<td>Fig 3</td>
</tr>
</tbody>
</table>
<p></p>
<p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 &micro;l per IP.</small></p>',
		'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
		'storage_buffer' => '',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
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		'slug' => '',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2019-09-10 14:30:49',
		'created' => '2017-05-08 13:56:13',
		'select_label' => '508 - AF9 polyclonal antibody (A1474-001 - Not determined - Human: positive. Other species: not tested. - Whole antiserum from rabbit containing 0.05% azide. - Rabbit)'
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			'name' => 'AF9 Antibody',
			'description' => '<p><strong>Other names:</strong> MLLT3, YEATS3</p>
<p>Polyclonal antibody raised in rabbit against human <strong>AF9</strong> <strong>(Super Elongation Complex Subunit)</strong> using a KLH conjugated synthetic peptide containing a sequence from the central region of the protein.</p>',
			'label1' => 'Validation data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chip.png" alt="AF9 Antibody ChIP Grade" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed on THP-1 cells using the Diagenode antibody against AF9 (Cat. No. C15310266). Sheared chromatin from 1 million cells and 2 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-a.png" alt="AF9 Antibody ChIP-seq Grade" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-b.png" alt="AF9 Antibody for ChIP-seq" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-c.png" alt="AF9 Antibody for ChIP-seq assay " /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-d.png" alt="AF9 Antibody validated in ChIP-seq" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-e.png" alt="AF9 Antibody ChIP-seq Grade" /></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 5 ChIP&rsquo;s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 400 kb region of chromosome 6 containing the PHIP positive control gene (fig 2A and B), and in 3 genomic regions surrounding the CDKN2C gene, the JMJD1C gene and HOX cluster on chromosome 1, 10 and 7, respectively (fig 2C, D and E).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-elisa.png" alt="AF9 Antibody ELISA Validation" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.</small></p>
</div>
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			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
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			'description' => '<h1><strong>Validated epigenetics antibodies</strong>&nbsp;&ndash; care for a sample?<br />&nbsp;</h1>
<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
<ul>
<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
<li><strong>Strict quality standards</strong>&nbsp;with rigorous QC and validation</li>
<li><strong>Classified</strong>&nbsp;based on level of validation for flexibility of application</li>
</ul>
<p>Existing sample sizes are listed below. We will soon expand our collection. &nbsp;Are you looking for a sample size of another antibody? Just <a href="mailto:agnieszka.zelisko@diagenode.com?Subject=Sample%20Size%20Request" target="_top">Contact us</a>.</p>',
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			'description' => '<div class="row">
<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
</div>
<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
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<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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			'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP',
			'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode',
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			'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
</div>
<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'description' => '<p><span style="font-weight: 400;">The list of Diagenode&rsquo;s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. &nbsp;Check the list below to see our targets.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Expert technical support</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Sample sizes available</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 100% satisfaction guarantee</span></li>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed on THP-1 cells using the Diagenode antibody against AF9 (Cat. No. C15310266). Sheared chromatin from 1 million cells and 2 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 5 ChIP&rsquo;s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 400 kb region of chromosome 6 containing the PHIP positive control gene (fig 2A and B), and in 3 genomic regions surrounding the CDKN2C gene, the JMJD1C gene and HOX cluster on chromosome 1, 10 and 7, respectively (fig 2C, D and E).</small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed on THP-1 cells using the Diagenode antibody against AF9 (Cat. No. C15310266). Sheared chromatin from 1 million cells and 2 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 5 ChIP&rsquo;s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 400 kb region of chromosome 6 containing the PHIP positive control gene (fig 2A and B), and in 3 genomic regions surrounding the CDKN2C gene, the JMJD1C gene and HOX cluster on chromosome 1, 10 and 7, respectively (fig 2C, D and E).</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.</small></p>
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<p>Diagenode has partnered with leading epigenetics experts and numerous epigenetics consortiums to bring to you a validated and comprehensive collection of epigenetic antibodies. As an expert in epigenetics, we are committed to offering highly-specific antibodies validated for ChIP/ChIP-seq and many other applications. All batch-specific validation data is available on our website.<br /><a href="../categories/antibodies">Read about our expertise in antibody production</a>.</p>
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<li><strong>Focused</strong> -&nbsp;Diagenode's selection of antibodies is exclusively dedicated for epigenetic research. <a title="See the full collection." href="../categories/all-antibodies">See the full collection.</a></li>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
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<p></p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed on THP-1 cells using the Diagenode antibody against AF9 (Cat. No. C15310266). Sheared chromatin from 1 million cells and 2 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).</small></p>
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	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'chip-qpcr-antibodies',
	'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
	'meta_title' => 'ChIP Quantitative PCR  Antibodies (ChIP-qPCR) | Diagenode',
	'modified' => '2016-01-20 11:30:24',
	'created' => '2015-10-20 11:45:36',
	'locale' => 'jpn'
)
$description = ''
$name = 'ChIP-qPCR (ab)'
$document = array(
	'id' => '969',
	'name' => 'AF9 polyclonal antibody',
	'description' => '<p>Polyclonal antibody raised in rabbit against human AF9 (Super Elongation Complex Subunit) using a KLHconjugated synthetic peptide containing a sequence from the central region of the protein.</p>',
	'image_id' => null,
	'type' => 'Datasheet',
	'url' => 'files/products/antibodies/Datasheet-af9-C15310266.pdf',
	'slug' => 'datasheet_heralpha_C15100066.pdf',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2017-10-12 16:16:12',
	'created' => '2017-10-12 14:52:53',
	'ProductsDocument' => array(
		'id' => '2434',
		'product_id' => '2909',
		'document_id' => '969'
	)
)
$sds = array(
	'id' => '3448',
	'name' => 'SDS C15310266 AF9 Antibody JP ja',
	'language' => 'ja',
	'url' => 'files/SDS/AF9/SDS-C15310266-AF9_Antibody-JP-ja-GHS_3_0.pdf',
	'countries' => 'JP',
	'modified' => '2024-01-16 13:12:19',
	'created' => '2024-01-16 13:12:19',
	'ProductsSafetySheet' => array(
		'id' => '5594',
		'product_id' => '2909',
		'safety_sheet_id' => '3448'
	)
)
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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AF9 polyclonal antibody

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