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AF9 polyclonal antibody

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

Antibody portal

Antibodies

Catalog Number

Format

Price

C15310266

100 μl

$380.00
Bulk order

Other format

C15310266-20

20 µl

Other names: MLLT3, YEATS3

Polyclonal antibody raised in rabbit against human AF9 (Super Elongation Complex Subunit) using a KLHconjugated synthetic peptide containing a sequence from the central region of the protein.

Lot	A1474-001
Concentration	Not determined
Species reactivity	Human: positive. Other species: not tested.
Type	Polyclonal ChIP grade, ChIP-seq grade
Purity	Whole antiserum from rabbit containing 0.05% azide.
Host	Rabbit
Storage Conditions	Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution/amount	References
ChIP/ChIP-seq *	2 µl/ChIP	Fig 1, 2
ELISA	1:100 - 1:500	Fig 3

*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 µl per IP.

Validation data

Figure 1. ChIP results obtained with the Diagenode antibody directed against AF9
ChIP was performed on THP-1 cells using the Diagenode antibody against AF9 (Cat. No. C15310266). Sheared chromatin from 1 million cells and 2 μl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).

Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AF9
ChIP was performed as described above. The IP’d DNA of 5 ChIP’s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 400 kb region of chromosome 6 containing the PHIP positive control gene (fig 2A and B), and in 3 genomic regions surrounding the CDKN2C gene, the JMJD1C gene and HOX cluster on chromosome 1, 10 and 7, respectively (fig 2C, D and E).

Figure 3. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.
Target Description
AF9 (UniProtKB/Swiss-Prot entry P42568) is a component of the super elongation complex which is required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA.
実験手法
ChIP-seq (ab)
Read more
ChIP-qPCR (ab)
Read more
資料
AF9 polyclonal antibody DATASHEET
Polyclonal antibody raised in rabbit against human AF9 (Super Elongation Complex Subunit) using a... Download

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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 5 ChIP&rsquo;s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 400 kb region of chromosome 6 containing the PHIP positive control gene (fig 2A and B), and in 3 genomic regions surrounding the CDKN2C gene, the JMJD1C gene and HOX cluster on chromosome 1, 10 and 7, respectively (fig 2C, D and E).</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.</small></p>
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<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chip.png" alt="AF9 Antibody ChIP Grade" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed on THP-1 cells using the Diagenode antibody against AF9 (Cat. No. C15310266). Sheared chromatin from 1 million cells and 2 &mu;l of antibody were used per ChIP experiment. QPCR was performed using primers specific for the indicated genes. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-b.png" alt="AF9 Antibody for ChIP-seq" /></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.</small></p>
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<td>ChIP/ChIP-seq *</td>
<td>2 &micro;l/ChIP</td>
<td>Fig 1, 2</td>
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<p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-10 &micro;l per IP.</small></p>',
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<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-elisa.png" alt="AF9 Antibody ELISA Validation" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.</small></p>
</div>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-b.png" alt="AF9 Antibody for ChIP-seq" /></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 5 ChIP&rsquo;s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 400 kb region of chromosome 6 containing the PHIP positive control gene (fig 2A and B), and in 3 genomic regions surrounding the CDKN2C gene, the JMJD1C gene and HOX cluster on chromosome 1, 10 and 7, respectively (fig 2C, D and E).</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.</small></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-c.png" alt="AF9 Antibody for ChIP-seq assay " /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-d.png" alt="AF9 Antibody validated in ChIP-seq" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-chipseq-e.png" alt="AF9 Antibody ChIP-seq Grade" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against AF9 </strong><br />ChIP was performed as described above. The IP&rsquo;d DNA of 5 ChIP&rsquo;s was pooled and analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 400 kb region of chromosome 6 containing the PHIP positive control gene (fig 2A and B), and in 3 genomic regions surrounding the CDKN2C gene, the JMJD1C gene and HOX cluster on chromosome 1, 10 and 7, respectively (fig 2C, D and E).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15310266-elisa.png" alt="AF9 Antibody ELISA Validation" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody directed against AF9 (Cat. No. C15310266). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8,000.</small></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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	'meta_description' => 'AF9 (Super Elongation Complex Subunit) Polyclonal Antibody validated in ELISA, ChIP-seq and ChIP-qPCR. Batch-specific data available on the website. Other names: MLLT3, YEATS3. Sample size available.',
	'modified' => '2024-01-16 13:13:18',
	'created' => '2017-10-12 16:10:59',
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$related_products = ''
$rrbs_service = array(
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	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
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}
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$country_code = 'US'
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	'id' => '2909',
	'antibody_id' => '508',
	'name' => 'AF9 Antibody (sample size)',
	'description' => '',
	'label1' => 'Validation Data',
	'info1' => '',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '20 µl',
	'catalog_number' => 'C15310266-20',
	'old_catalog_number' => '',
	'sf_code' => 'C15310266-361',
	'type' => 'FRE',
	'search_order' => '',
	'price_EUR' => '105',
	'price_USD' => '115',
	'price_GBP' => '100',
	'price_JPY' => '16450',
	'price_CNY' => '9500',
	'price_AUD' => '288',
	'country' => 'ALL',
	'except_countries' => 'None',
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	'slug' => 'af9-polyclonal-antibody-20-ul',
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	'meta_keywords' => '',
	'meta_description' => 'AF9 (Super Elongation Complex Subunit) Polyclonal Antibody validated in ELISA, ChIP-seq and ChIP-qPCR. Batch-specific data available on the website. Other names: MLLT3, YEATS3. Sample size available.',
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	'created' => '2017-10-12 16:10:59',
	'ProductsGroup' => array(
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		'product_id' => '2909',
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)
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$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
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	'parent_id' => '40',
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	'description' => '',
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	'online' => true,
	'tabular' => true,
	'slug' => 'chip-qpcr-antibodies',
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	'meta_title' => 'ChIP Quantitative PCR  Antibodies (ChIP-qPCR) | Diagenode',
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	'parent_id' => '40',
	'name' => 'ChIP-qPCR (ab)',
	'description' => '',
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	'online' => true,
	'tabular' => true,
	'slug' => 'chip-qpcr-antibodies',
	'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin',
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	'meta_title' => 'ChIP Quantitative PCR  Antibodies (ChIP-qPCR) | Diagenode',
	'modified' => '2016-01-20 11:30:24',
	'created' => '2015-10-20 11:45:36',
	'locale' => 'jpn'
)
$description = ''
$name = 'ChIP-qPCR (ab)'
$document = array(
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	'name' => 'AF9 polyclonal antibody',
	'description' => '<p>Polyclonal antibody raised in rabbit against human AF9 (Super Elongation Complex Subunit) using a KLHconjugated synthetic peptide containing a sequence from the central region of the protein.</p>',
	'image_id' => null,
	'type' => 'Datasheet',
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	'slug' => 'datasheet_heralpha_C15100066.pdf',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2017-10-12 16:16:12',
	'created' => '2017-10-12 14:52:53',
	'ProductsDocument' => array(
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		'product_id' => '2907',
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)
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	'language' => 'ja',
	'url' => 'files/SDS/AF9/SDS-C15310266-AF9_Antibody-JP-ja-GHS_3_0.pdf',
	'countries' => 'JP',
	'modified' => '2024-01-16 13:12:19',
	'created' => '2024-01-16 13:12:19',
	'ProductsSafetySheet' => array(
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		'product_id' => '2907',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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AF9 polyclonal antibody

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