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CBX2 Antibody - ChIP-seq Grade (sample size)

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

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Catalog Number

Format

Price

C15410339-20

20 µl

$120.00
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Other format

C15410339

100 μl

Polyclonal antibody raised in rabbit against human CBX2 (chromobox 2), using a synthetic peptide containing a sequence from the C-terminus of the protein¹.

Lot	A302-524A3
Concentration	100 μl
Species reactivity	Human: positive. Other species: not tested.
Type	Polyclonal, ChIP grade, ChIP-seq grade
Purity	Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide.
Host	Rabbit
Storage Conditions	Store at 4°C.
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ChIP/ChIP-seq ^*	1 μg/ChIP	Fig 1, 2
Western Blotting	1:1,000	Fig 3
IP	6 μg per IP	Fig 4

^* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

¹Manufactured by Bethyl Laboratories, Inc., Texas, USA

Validation data

Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX2
ChIP assays were performed using K562 cells, the Diagenode antibody against CBX2 (Cat. No. C15410339) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ARNTL2 gene, used as positive control, and for the promoter of the GAPDH gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX2
ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ARNTL2 positive control gene and the HOXA9 cluster (fig 2C and D).

Figure 3. Western blot analysis using the Diagenode antibody directed against CBX2
Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX2 (Cat. No. C15410339) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2
Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.
Target Description
CBX2 (UniProtKB/Swiss-Prot entry Q14781) is a component of the PRC1-like polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. The PRC1 complex mediates monoubiquitination of histone H2A on lysine 119, introducing heritably changed expression. CBX2 is involved in sexual development. Mutations in CBX2 are associated with gonadal dysgenesis in humans.

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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CBX2 (Cat. No. C15410339) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ARNTL2 gene, used as positive control, and for the promoter of the GAPDH gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<td>1 &mu;g/ChIP</td>
<td>Fig 1, 2</td>
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<td>Western Blotting</td>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-a.jpg" alt="CBX2 Antibody ChIP-seq Grade" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 &mu;g of the Diagenode antibody against CBX2 (Cat. No. C15410339) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ARNTL2 positive control gene and the HOXA9 cluster (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CBX2</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX2 (Cat. No. C15410339) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-ip.jpg" alt="CBX2 Antibody validated in Immunoprecipitation" /></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 &mu;g of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CBX2 (Cat. No. C15410339) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ARNTL2 gene, used as positive control, and for the promoter of the GAPDH gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-a.jpg" alt="CBX2 Antibody ChIP-seq Grade" /></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-c.jpg" alt="CBX2 Antibody for ChIP-seq assay " /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 &mu;g of the Diagenode antibody against CBX2 (Cat. No. C15410339) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ARNTL2 positive control gene and the HOXA9 cluster (fig 2C and D).</small></p>
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<div class="small-3 columns">
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CBX2</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX2 (Cat. No. C15410339) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-ip.jpg" alt="CBX2 Antibody validated in Immunoprecipitation" /></p>
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<div class="small-9 columns">
<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 &mu;g of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 &mu;g of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.</small></p>
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View::render() - CORE/Cake/View/View.php, line 473
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ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CBX2 (Cat. No. C15410339) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ARNTL2 gene, used as positive control, and for the promoter of the GAPDH gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-a.jpg" alt="CBX2 Antibody ChIP-seq Grade" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-b.jpg" alt="CBX2 Antibody for ChIP-seq " /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-c.jpg" alt="CBX2 Antibody for ChIP-seq assay " /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-d.jpg" alt="CBX2 Antibody validated in ChIP-seq " /></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 &mu;g of the Diagenode antibody against CBX2 (Cat. No. C15410339) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ARNTL2 positive control gene and the HOXA9 cluster (fig 2C and D).</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-wb.jpg" alt="CBX2 Antibody validated in Western Blot" /></p>
</div>
<div class="small-9 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CBX2</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX2 (Cat. No. C15410339) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-ip.jpg" alt="CBX2 Antibody validated in Immunoprecipitation" /></p>
</div>
<div class="small-9 columns">
<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 &mu;g of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.</small></p>
</div>
</div>',
		'label2' => 'Target Description',
		'info2' => '<p>CBX2 (UniProtKB/Swiss-Prot entry Q14781) is a component of the PRC1-like polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. The PRC1 complex mediates monoubiquitination of histone H2A on lysine 119, introducing heritably changed expression. CBX2 is involved in sexual development. Mutations in CBX2 are associated with gonadal dysgenesis in humans.</p>',
		'label3' => '',
		'info3' => '',
		'format' => '20 µl',
		'catalog_number' => 'C15410339-20',
		'old_catalog_number' => '',
		'sf_code' => 'C15410339-361',
		'type' => 'REF',
		'search_order' => '03-Antibody',
		'price_EUR' => '110',
		'price_USD' => '120',
		'price_GBP' => '105',
		'price_JPY' => '17230',
		'price_CNY' => '',
		'price_AUD' => '300',
		'country' => 'ALL',
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		'slug' => 'cbx2-polyclonal-antibody-20',
		'meta_title' => '',
		'meta_keywords' => '',
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		'modified' => '2024-01-17 19:09:57',
		'created' => '2020-12-30 13:49:36',
		'locale' => 'jpn'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => '638',
		'name' => 'CBX2 Antibody - ChIP-seq Grade (sample size)',
		'description' => 'CBX2 (UniProtKB/Swiss-Prot entry Q14781) is a component of the PRC1-like polycomb multiprotein complex, which is required
to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and
modification of histones. The PRC1 complex mediates monoubiquitination of histone H2A on lysine 119, introducing heritably
changed expression. CBX2 is involved in sexual development. Mutations in CBX2 are associated with gonadal dysgenesis in
humans.',
		'clonality' => '',
		'isotype' => '',
		'lot' => 'A302-524A3',
		'concentration' => '100 μl',
		'reactivity' => 'Human: positive. Other species: not tested.',
		'type' => 'Polyclonal, <strong>ChIP grade, ChIP-seq grade</strong>',
		'purity' => 'Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide.',
		'classification' => '',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>1 &mu;g/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 3</td>
</tr>
<tr>
<td>IP</td>
<td>6 &mu;g per IP</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 &mu;g per IP.</small></p>
<p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>',
		'storage_conditions' => 'Store at 4°C.',
		'storage_buffer' => '',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
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		'slug' => 'cbx2-polyclonal-antibody-20',
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		'modified' => '2020-12-30 13:46:11',
		'created' => '2020-12-30 13:46:11',
		'select_label' => '638 - CBX2 Antibody - ChIP-seq Grade (sample size) (A302-524A3 - 100 μl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide. - Rabbit)'
	),
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			'created' => '2020-12-30 13:50:01'
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		'Master' => array(
			'id' => '2918',
			'antibody_id' => '513',
			'name' => 'CBX2 Antibody',
			'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CBX2</strong> <strong>(chromobox 2)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>',
			'label1' => 'Validation data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chip.jpg" alt="CBX2 Antibody ChIP Grade" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CBX2 (Cat. No. C15410339) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ARNTL2 gene, used as positive control, and for the promoter of the GAPDH gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-a.jpg" alt="CBX2 Antibody ChIP-seq Grade" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-b.jpg" alt="CBX2 Antibody for ChIP-seq " /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-c.jpg" alt="CBX2 Antibody for ChIP-seq assay " /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-d.jpg" alt="CBX2 Antibody validated in ChIP-seq " /></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 &mu;g of the Diagenode antibody against CBX2 (Cat. No. C15410339) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ARNTL2 positive control gene and the HOXA9 cluster (fig 2C and D).</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-wb.jpg" alt="CBX2 Antibody validated in Western Blot" /></p>
</div>
<div class="small-9 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CBX2</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX2 (Cat. No. C15410339) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-ip.jpg" alt="CBX2 Antibody validated in Immunoprecipitation" /></p>
</div>
<div class="small-9 columns">
<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 &mu;g of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.</small></p>
</div>
</div>',
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			'price_CNY' => '0',
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			'meta_title' => 'CBX2 Antibody  - ChIP-seq Grade (C15410339) | Diagenode',
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			'meta_description' => 'CBX2 (Chromobox 2) Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website.',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
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			'name' => 'ChIP-grade antibodies',
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<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
</div>
<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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			'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP',
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			'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
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<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'name' => 'All antibodies',
			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
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			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CBX2 (Cat. No. C15410339) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ARNTL2 gene, used as positive control, and for the promoter of the GAPDH gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 &mu;g of the Diagenode antibody against CBX2 (Cat. No. C15410339) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ARNTL2 positive control gene and the HOXA9 cluster (fig 2C and D).</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 &mu;g of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.</small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CBX2 (Cat. No. C15410339) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ARNTL2 gene, used as positive control, and for the promoter of the GAPDH gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CBX2</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX2 (Cat. No. C15410339) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 &mu;g of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.</small></p>
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<td>Fig 4</td>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CBX2 (Cat. No. C15410339) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ARNTL2 gene, used as positive control, and for the promoter of the GAPDH gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<div class="small-12 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-a.jpg" alt="CBX2 Antibody ChIP-seq Grade" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-b.jpg" alt="CBX2 Antibody for ChIP-seq " /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-c.jpg" alt="CBX2 Antibody for ChIP-seq assay " /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-d.jpg" alt="CBX2 Antibody validated in ChIP-seq " /></p>
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<div class="small-12 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 &mu;g of the Diagenode antibody against CBX2 (Cat. No. C15410339) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ARNTL2 positive control gene and the HOXA9 cluster (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CBX2</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX2 (Cat. No. C15410339) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<div class="small-3 columns">
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 &mu;g of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
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Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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<td>1 &mu;g/ChIP</td>
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		'name' => 'CBX2 Antibody - ChIP-seq Grade (sample size)',
		'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CBX2</strong> <strong>(chromobox 2)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chip.jpg" alt="CBX2 Antibody ChIP Grade" /></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CBX2 (Cat. No. C15410339) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ARNTL2 gene, used as positive control, and for the promoter of the GAPDH gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-a.jpg" alt="CBX2 Antibody ChIP-seq Grade" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-b.jpg" alt="CBX2 Antibody for ChIP-seq " /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-c.jpg" alt="CBX2 Antibody for ChIP-seq assay " /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 &mu;g of the Diagenode antibody against CBX2 (Cat. No. C15410339) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ARNTL2 positive control gene and the HOXA9 cluster (fig 2C and D).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-wb.jpg" alt="CBX2 Antibody validated in Western Blot" /></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CBX2</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX2 (Cat. No. C15410339) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-ip.jpg" alt="CBX2 Antibody validated in Immunoprecipitation" /></p>
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<div class="small-9 columns">
<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 &mu;g of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.</small></p>
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		'description' => 'CBX2 (UniProtKB/Swiss-Prot entry Q14781) is a component of the PRC1-like polycomb multiprotein complex, which is required
to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and
modification of histones. The PRC1 complex mediates monoubiquitination of histone H2A on lysine 119, introducing heritably
changed expression. CBX2 is involved in sexual development. Mutations in CBX2 are associated with gonadal dysgenesis in
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		'isotype' => '',
		'lot' => 'A302-524A3',
		'concentration' => '100 μl',
		'reactivity' => 'Human: positive. Other species: not tested.',
		'type' => 'Polyclonal, <strong>ChIP grade, ChIP-seq grade</strong>',
		'purity' => 'Affinity purified polyclonal antibody in Tris-citrate buffer containing 0.09% azide.',
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		'application_table' => '<table>
<thead>
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<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
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<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>1 &mu;g/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Western Blotting</td>
<td>1:1,000</td>
<td>Fig 3</td>
</tr>
<tr>
<td>IP</td>
<td>6 &mu;g per IP</td>
<td>Fig 4</td>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 &mu;g per IP.</small></p>
<p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>',
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			'antibody_id' => '513',
			'name' => 'CBX2 Antibody',
			'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CBX2</strong> <strong>(chromobox 2)</strong>, using a synthetic peptide containing a sequence from the C-terminus of the protein<sup>1</sup>.</p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chip.jpg" alt="CBX2 Antibody ChIP Grade" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CBX2 (Cat. No. C15410339) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ARNTL2 gene, used as positive control, and for the promoter of the GAPDH gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<div class="row">
<div class="small-12 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-a.jpg" alt="CBX2 Antibody ChIP-seq Grade" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-b.jpg" alt="CBX2 Antibody for ChIP-seq " /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-c.jpg" alt="CBX2 Antibody for ChIP-seq assay " /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-d.jpg" alt="CBX2 Antibody validated in ChIP-seq " /></p>
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<div class="row">
<div class="small-12 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 &mu;g of the Diagenode antibody against CBX2 (Cat. No. C15410339) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ARNTL2 positive control gene and the HOXA9 cluster (fig 2C and D).</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-wb.jpg" alt="CBX2 Antibody validated in Western Blot" /></p>
</div>
<div class="small-9 columns">
<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CBX2</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX2 (Cat. No. C15410339) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-ip.jpg" alt="CBX2 Antibody validated in Immunoprecipitation" /></p>
</div>
<div class="small-9 columns">
<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 &mu;g of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
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<p></p>
<p></p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CBX2 (Cat. No. C15410339) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ARNTL2 gene, used as positive control, and for the promoter of the GAPDH gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 &mu;g of the Diagenode antibody against CBX2 (Cat. No. C15410339) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ARNTL2 positive control gene and the HOXA9 cluster (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CBX2</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX2 (Cat. No. C15410339) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 &mu;g of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CBX2 (Cat. No. C15410339) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ARNTL2 gene, used as positive control, and for the promoter of the GAPDH gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 &mu;g of the Diagenode antibody against CBX2 (Cat. No. C15410339) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ARNTL2 positive control gene and the HOXA9 cluster (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CBX2</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX2 (Cat. No. C15410339) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-ip.jpg" alt="CBX2 Antibody validated in Immunoprecipitation" /></p>
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<div class="small-9 columns">
<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 &mu;g of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.</small></p>
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Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download
CBX2 polyclonal antibody DATASHEET Polyclonal antibody raised in rabbit against human CBX2 (chromobox 2), using a synthetic peptide ...	Download

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CBX2 Antibody - ChIP-seq Grade (sample size)

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