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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chip.jpg" alt="CBX2 Antibody ChIP Grade" /></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CBX2 (Cat. No. C15410339) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ARNTL2 gene, used as positive control, and for the promoter of the GAPDH gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ARNTL2 positive control gene and the HOXA9 cluster (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CBX2</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX2 (Cat. No. C15410339) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.</small></p>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CBX2 (Cat. No. C15410339) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ARNTL2 gene, used as positive control, and for the promoter of the GAPDH gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ARNTL2 positive control gene and the HOXA9 cluster (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CBX2</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX2 (Cat. No. C15410339) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.</small></p>
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<td>ChIP/ChIP-seq <sup>*</sup></td>
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<td>Fig 1, 2</td>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP assays were performed using K562 cells, the Diagenode antibody against CBX2 (Cat. No. C15410339) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ARNTL2 gene, used as positive control, and for the promoter of the GAPDH gene, used as negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-a.jpg" alt="CBX2 Antibody ChIP-seq Grade" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-b.jpg" alt="CBX2 Antibody for ChIP-seq " /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ARNTL2 positive control gene and the HOXA9 cluster (fig 2C and D).</small></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CBX2</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX2 (Cat. No. C15410339) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.</small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-b.jpg" alt="CBX2 Antibody for ChIP-seq " /></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-chipseq-d.jpg" alt="CBX2 Antibody validated in ChIP-seq " /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX2</strong><br />ChIP was performed on sheared chromatin from 4 million K562 cells using 1 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1.5 Mb region of the human chromosome 1 (fig 2A and B), and in two genomic regions surrounding the ARNTL2 positive control gene and the HOXA9 cluster (fig 2C and D).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-wb.jpg" alt="CBX2 Antibody validated in Western Blot" /></p>
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<p><small><strong> Figure 3. Western blot analysis using the Diagenode antibody directed against CBX2</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX2 (Cat. No. C15410339) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410339-ip.jpg" alt="CBX2 Antibody validated in Immunoprecipitation" /></p>
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<p><small><strong> Figure 4. Immunoprecipitation using the Diagenode antibody directed against CBX2</strong><br />Immunoprecipitation was performed on whole cell extracts from 293T cells using 6 μg of the Diagenode antibody against CBX2 (Cat. No. C15410339, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX2 protein was detected by western blot with the CBX2 antibody diluted 1:1,000.</small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
'id' => '3177',
'antibody_id' => '638',
'name' => 'CBX2 Antibody (sample size)',
'description' => '',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '20 µl',
'catalog_number' => 'C15410339-20',
'old_catalog_number' => '',
'sf_code' => 'C15410339-361',
'type' => 'REF',
'search_order' => '03-Antibody',
'price_EUR' => '110',
'price_USD' => '120',
'price_GBP' => '105',
'price_JPY' => '17230',
'price_CNY' => '',
'price_AUD' => '300',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => false,
'last_datasheet_update' => '',
'slug' => 'cbx2-polyclonal-antibody-20',
'meta_title' => '',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2024-01-17 19:09:57',
'created' => '2020-12-30 13:49:36',
'ProductsGroup' => array(
'id' => '381',
'product_id' => '3177',
'group_id' => '340'
)
)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
'id' => '30',
'position' => '10',
'parent_id' => '40',
'name' => 'IP',
'description' => '<p>Immunoprecipitation</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunoprecipitation',
'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications',
'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-01-13 12:23:07',
'created' => '2015-07-08 13:46:50',
'ProductsApplication' => array(
'id' => '4743',
'product_id' => '2918',
'application_id' => '30'
)
)
$slugs = array(
(int) 0 => 'immunoprecipitation'
)
$applications = array(
'id' => '30',
'position' => '10',
'parent_id' => '40',
'name' => 'IP',
'description' => '<p>Immunoprecipitation</p>',
'in_footer' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'slug' => 'immunoprecipitation',
'meta_keywords' => 'Immunoprecipitation,Monoclonal antibody,Polyclonal antibody',
'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for Immunoprecipitation applications',
'meta_title' => 'Immunoprecipitation - Monoclonal antibody - Polyclonal antibody | Diagenode',
'modified' => '2016-01-13 12:23:07',
'created' => '2015-07-08 13:46:50',
'locale' => 'jpn'
)
$description = '<p>Immunoprecipitation</p>'
$name = 'IP'
$document = array(
'id' => '976',
'name' => 'CBX2 polyclonal antibody',
'description' => '<p>Polyclonal antibody raised in rabbit against human CBX2 (chromobox 2), using a synthetic peptide containing a sequence from the C-terminus of the protein.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/antibodies/Datasheet_CBX2_C15410339.pdf',
'slug' => 'cbx2-polyclonal-antibody',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2018-01-03 10:23:27',
'created' => '2018-01-03 10:23:27',
'ProductsDocument' => array(
'id' => '2476',
'product_id' => '2918',
'document_id' => '976'
)
)
$sds = array(
'id' => '3769',
'name' => 'SDS C15410339 CBX2 Antibody ES es',
'language' => 'es',
'url' => 'files/SDS/CBX2/SDS-C15410339-CBX2_Antibody-ES-es-GHS_2_0.pdf',
'countries' => 'ES',
'modified' => '2024-01-17 19:09:03',
'created' => '2024-01-17 19:09:03',
'ProductsSafetySheet' => array(
'id' => '6157',
'product_id' => '2918',
'safety_sheet_id' => '3769'
)
)
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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