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CBX8 polyclonal antibody

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

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Catalog Number

Format

Price

C15410333

100 μl

$460.00
Bulk order

Other format

C15410333-20

20 µl

Polyclonal antibody raised in rabbit against human CBX8 (chromobox 8), using a synthetic peptide containing a sequence from the C-terminal part of the protein.

1 Manufactured by Bethyl Laboratories, Inc., Texas, USA

Lot	A300-882A2
Concentration	1 μg/μl
Species reactivity	Human: positive. Other species: not tested.
Type	Polyclonal ChIP grade, ChIP-seq grade
Purity	Affinity purified polyclonal antibody.
Host	Rabbit
Storage Conditions	Store at 4°C
Storage Buffer	Tris-citrate/phosphate buffer containing 0.09% azide.
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ChIP/ChIP-seq *	1-2 µg/ChIP	Fig 1, 2
Western blot	1:1,000	Fig 3
IP	6 µg per IP	Fig 4

* Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per ChIP.

¹Manufactured by Bethyl Laboratories, Inc., Texas, USA

Validation data

Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX8
ChIP assays were performed using K562 cells, the Diagenode antibody against CBX8 (Cat. No. C15410333) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for, the tRNA-Ala gene, used as positive control, and for the promoters of the active ACTB and EIF4A2 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

A.
B.
C.
D.
E.

Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX8
ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against CBX8 (Cat. No. C15410333) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human chromosome 1 (fig 2A and B), and along the short arm and two zoomins of chromosome 6 (fig 2C and D). The position of the amplicon used for ChIP-qPCR validation is indicated by an arrow.

Figure 3. Western blot analysis using the Diagenode antibody directed against CBX8
Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX8 (Cat. No. C15410333) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CBX8
Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against CBX8 (Cat. No. C15410333, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX8 protein was detected by western blot with the CBX8 antibody diluted 1:2,500.
Target Description
CBX8 (UniProtKB/Swiss-Prot entry Q9HC52) is a component of the polycomb group PRC1-like multiprotein complex. This complex is required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. It mediates monoubiquitination of histone H2A Lys-119, which leads to recruitment of PRC2 and methylation of H3K27 and H3K9.

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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CBX8 (Cat. No. C15410333) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &micro;g of antibody per ChIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for, the tRNA-Ala gene, used as positive control, and for the promoters of the active ACTB and EIF4A2 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP was performed on sheared chromatin from 4 million K562 cells using 2 &micro;g of the Diagenode antibody against CBX8 (Cat. No. C15410333) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human chromosome 1 (fig 2A and B), and along the short arm and two zoomins of chromosome 6 (fig 2C and D). The position of the amplicon used for ChIP-qPCR validation is indicated by an arrow.</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CBX8</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX8 (Cat. No. C15410333) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CBX8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 &micro;g of the Diagenode antibody against CBX8 (Cat. No. C15410333, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX8 protein was detected by western blot with the CBX8 antibody diluted 1:2,500. </small></p>
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<td>Fig 4</td>
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<p><small>* Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 &mu;g per ChIP.</small></p>
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<p><small>1 Manufactured by Bethyl Laboratories, Inc., Texas, USA<br /></small></p>',
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CBX8 (Cat. No. C15410333) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &micro;g of antibody per ChIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for, the tRNA-Ala gene, used as positive control, and for the promoters of the active ACTB and EIF4A2 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP was performed on sheared chromatin from 4 million K562 cells using 2 &micro;g of the Diagenode antibody against CBX8 (Cat. No. C15410333) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human chromosome 1 (fig 2A and B), and along the short arm and two zoomins of chromosome 6 (fig 2C and D). The position of the amplicon used for ChIP-qPCR validation is indicated by an arrow.</small></p>
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<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-ip.png" alt="CBX8 Antibody validated in Immunoprecipitation" /></div>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CBX8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 &micro;g of the Diagenode antibody against CBX8 (Cat. No. C15410333, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX8 protein was detected by western blot with the CBX8 antibody diluted 1:2,500. </small></p>
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<td>1:1,000</td>
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<p><small>* Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 &mu;g per ChIP.</small></p>
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<div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-chip.png" alt="CBX8 Antibody ChIP Grade" /></div>
<div class="small-8 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CBX8 (Cat. No. C15410333) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &micro;g of antibody per ChIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for, the tRNA-Ala gene, used as positive control, and for the promoters of the active ACTB and EIF4A2 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<div class="row">
<div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-a.jpg" alt="CBX8 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-b.jpg" alt="CBX8 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-c.jpg" alt="CBX8 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-d.jpg" alt="CBX8 Antibody validated in ChIP-seq" /><br /> E.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-e.jpg" alt="CBX8 Antibody ChIP-seq Grade" /></div>
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<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP was performed on sheared chromatin from 4 million K562 cells using 2 &micro;g of the Diagenode antibody against CBX8 (Cat. No. C15410333) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human chromosome 1 (fig 2A and B), and along the short arm and two zoomins of chromosome 6 (fig 2C and D). The position of the amplicon used for ChIP-qPCR validation is indicated by an arrow.</small></p>
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<div class="row">
<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-wb.png" alt="CBX8 Antibody validated in Western Blot" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CBX8</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX8 (Cat. No. C15410333) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-ip.png" alt="CBX8 Antibody validated in Immunoprecipitation" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CBX8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 &micro;g of the Diagenode antibody against CBX8 (Cat. No. C15410333, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX8 protein was detected by western blot with the CBX8 antibody diluted 1:2,500. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<p><small>1 Manufactured by Bethyl Laboratories, Inc., Texas, USA<br /></small></p>',
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<div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-chip.png" alt="CBX8 Antibody ChIP Grade" /></div>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CBX8 (Cat. No. C15410333) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &micro;g of antibody per ChIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for, the tRNA-Ala gene, used as positive control, and for the promoters of the active ACTB and EIF4A2 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-a.jpg" alt="CBX8 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-b.jpg" alt="CBX8 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-c.jpg" alt="CBX8 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-d.jpg" alt="CBX8 Antibody validated in ChIP-seq" /><br /> E.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-e.jpg" alt="CBX8 Antibody ChIP-seq Grade" /></div>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP was performed on sheared chromatin from 4 million K562 cells using 2 &micro;g of the Diagenode antibody against CBX8 (Cat. No. C15410333) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human chromosome 1 (fig 2A and B), and along the short arm and two zoomins of chromosome 6 (fig 2C and D). The position of the amplicon used for ChIP-qPCR validation is indicated by an arrow.</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CBX8</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX8 (Cat. No. C15410333) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CBX8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 &micro;g of the Diagenode antibody against CBX8 (Cat. No. C15410333, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX8 protein was detected by western blot with the CBX8 antibody diluted 1:2,500. </small></p>
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<p><small>* Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 &mu;g per ChIP.</small></p>
<p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>',
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<div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-chip.png" alt="CBX8 Antibody ChIP Grade" /></div>
<div class="small-8 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CBX8 (Cat. No. C15410333) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &micro;g of antibody per ChIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for, the tRNA-Ala gene, used as positive control, and for the promoters of the active ACTB and EIF4A2 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-a.jpg" alt="CBX8 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-b.jpg" alt="CBX8 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-c.jpg" alt="CBX8 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-d.jpg" alt="CBX8 Antibody validated in ChIP-seq" /><br /> E.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-e.jpg" alt="CBX8 Antibody ChIP-seq Grade" /></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP was performed on sheared chromatin from 4 million K562 cells using 2 &micro;g of the Diagenode antibody against CBX8 (Cat. No. C15410333) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human chromosome 1 (fig 2A and B), and along the short arm and two zoomins of chromosome 6 (fig 2C and D). The position of the amplicon used for ChIP-qPCR validation is indicated by an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-wb.png" alt="CBX8 Antibody validated in Western Blot" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CBX8</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX8 (Cat. No. C15410333) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-ip.png" alt="CBX8 Antibody validated in Immunoprecipitation" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CBX8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 &micro;g of the Diagenode antibody against CBX8 (Cat. No. C15410333, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX8 protein was detected by western blot with the CBX8 antibody diluted 1:2,500. </small></p>
</div>
</div>',
		'label2' => 'Target Description',
		'info2' => '<p><strong>CBX8</strong> (UniProtKB/Swiss-Prot entry Q9HC52) is a component of the polycomb group PRC1-like multiprotein complex. This complex is required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. It mediates monoubiquitination of histone H2A Lys-119, which leads to recruitment of PRC2 and methylation of H3K27 and H3K9.</p>',
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		'sf_code' => 'C15410333-D001-001161',
		'type' => 'REF',
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		'price_EUR' => '435',
		'price_USD' => '460',
		'price_GBP' => '380',
		'price_JPY' => '68145',
		'price_CNY' => '',
		'price_AUD' => '1150',
		'country' => 'ALL',
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		'last_datasheet_update' => 'January 13, 2017',
		'slug' => 'cbx8-polyclonal-antibody-100-ul',
		'meta_title' => 'CBX8 polyclonal antibody | Diagenode',
		'meta_keywords' => 'CBX8 (chromobox 8) polyclonal antibody,Immunoprecipitation,Western blot',
		'meta_description' => 'CBX8 (chromobox 8) polyclonal antibody from diagenode raised in rabbit against human has been validated for use in Immunoprecipitation and Western blot',
		'modified' => '2024-01-17 18:39:16',
		'created' => '2016-12-12 16:06:57',
		'locale' => 'jpn'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => '493',
		'name' => 'CBX8 polyclonal antibody',
		'description' => 'CBX8 (UniProtKB/Swiss-Prot entry Q9HC52) is a component of the polycomb group PRC1-like multiprotein complex. This complex is required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. It mediates monoubiquitination of histone H2A Lys-119, which leads to recruitment of PRC2 and methylation of H3K27 and H3K9.',
		'clonality' => '',
		'isotype' => '',
		'lot' => 'A300-882A2',
		'concentration' => '1 μg/μl',
		'reactivity' => 'Human: positive. Other species: not tested.',
		'type' => 'Polyclonal <strong>ChIP grade, ChIP-seq grade</strong>',
		'purity' => 'Affinity purified polyclonal antibody.',
		'classification' => 'Classic',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq *</td>
<td>1-2 &micro;g/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Western blot</td>
<td>1:1,000</td>
<td>Fig 3</td>
</tr>
<tr>
<td>IP</td>
<td>6 &micro;g per IP</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p></p>
<p><small>* Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 &mu;g per ChIP.</small></p>
<p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>',
		'storage_conditions' => 'Store at 4°C',
		'storage_buffer' => 'Tris-citrate/phosphate buffer containing 0.09% azide.',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
		'uniprot_acc' => '',
		'slug' => 'cbx8-polyclonal-antibody',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2021-01-06 10:20:15',
		'created' => '2016-12-12 16:18:24',
		'select_label' => '493 - CBX8 polyclonal antibody (A300-882A2 - 1 μg/μl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody. - Rabbit)'
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			'name' => 'CBX8 Antibody',
			'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CBX8 (chromobox 8)</strong>, using a synthetic peptide containing a sequence from the C-terminal part of the protein.</p>',
			'label1' => 'Validation data',
			'info1' => '<div class="row">
<div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-chip.png" alt="CBX8 Antibody ChIP Grade" /></div>
<div class="small-8 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CBX8 (Cat. No. C15410333) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &micro;g of antibody per ChIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for, the tRNA-Ala gene, used as positive control, and for the promoters of the active ACTB and EIF4A2 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-a.jpg" alt="CBX8 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-b.jpg" alt="CBX8 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-c.jpg" alt="CBX8 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-d.jpg" alt="CBX8 Antibody validated in ChIP-seq" /><br /> E.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-e.jpg" alt="CBX8 Antibody ChIP-seq Grade" /></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP was performed on sheared chromatin from 4 million K562 cells using 2 &micro;g of the Diagenode antibody against CBX8 (Cat. No. C15410333) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human chromosome 1 (fig 2A and B), and along the short arm and two zoomins of chromosome 6 (fig 2C and D). The position of the amplicon used for ChIP-qPCR validation is indicated by an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-wb.png" alt="CBX8 Antibody validated in Western Blot" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CBX8</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX8 (Cat. No. C15410333) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-ip.png" alt="CBX8 Antibody validated in Immunoprecipitation" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CBX8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 &micro;g of the Diagenode antibody against CBX8 (Cat. No. C15410333, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX8 protein was detected by western blot with the CBX8 antibody diluted 1:2,500. </small></p>
</div>
</div>',
			'label2' => 'Target Description',
			'info2' => '<p><strong>CBX8</strong> (UniProtKB/Swiss-Prot entry Q9HC52) is a component of the polycomb group PRC1-like multiprotein complex. This complex is required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. It mediates monoubiquitination of histone H2A Lys-119, which leads to recruitment of PRC2 and methylation of H3K27 and H3K9.</p>',
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			'price_EUR' => '435',
			'price_USD' => '460',
			'price_GBP' => '380',
			'price_JPY' => '68145',
			'price_CNY' => '',
			'price_AUD' => '1150',
			'country' => 'ALL',
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			'meta_title' => 'CBX8 Antibody - ChIP-seq Grade (C15410333) | Diagenode',
			'meta_keywords' => 'CBX8 (chromobox 8) polyclonal antibody,Immunoprecipitation,Western blot',
			'meta_description' => 'CBX8 (Chromobox 8) Polyclonal Aantibody validated  in ChIP-seq, ChIP-qPCR, IP and WB. Batch-specific data available on the website. ',
			'modified' => '2024-01-17 18:39:16',
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			'slug' => 'chip-qpcr-antibodies',
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			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP-qPCR applications',
			'meta_title' => 'ChIP Quantitative PCR  Antibodies (ChIP-qPCR) | Diagenode',
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			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
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			'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for ChIP Sequencing applications',
			'meta_title' => 'ChIP Sequencing Antibodies (ChIP-Seq) | Diagenode',
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			'name' => 'All antibodies',
			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
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			'description' => '<p><span style="font-weight: 400;">Diagenode offers the large number of antibodies raised against histone modifying enzymes. The list below includes the antibodies against enzymes like: histone deacetylases, histone demethylases, histone transferases.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'meta_title' => 'Histone modifying enzymes - Antibodies | Diagenode',
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			'name' => 'ChIP-grade antibodies',
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<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
</div>
<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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			'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP',
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
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ReflectionMethod::invokeArgs() - [internal], line ??
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<p><small>1 Manufactured by Bethyl Laboratories, Inc., Texas, USA<br /></small></p>',
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CBX8 (Cat. No. C15410333) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &micro;g of antibody per ChIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for, the tRNA-Ala gene, used as positive control, and for the promoters of the active ACTB and EIF4A2 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP was performed on sheared chromatin from 4 million K562 cells using 2 &micro;g of the Diagenode antibody against CBX8 (Cat. No. C15410333) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human chromosome 1 (fig 2A and B), and along the short arm and two zoomins of chromosome 6 (fig 2C and D). The position of the amplicon used for ChIP-qPCR validation is indicated by an arrow.</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CBX8</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX8 (Cat. No. C15410333) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<div class="small-9 columns">
<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CBX8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 &micro;g of the Diagenode antibody against CBX8 (Cat. No. C15410333, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX8 protein was detected by western blot with the CBX8 antibody diluted 1:2,500. </small></p>
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<p><small>1 Manufactured by Bethyl Laboratories, Inc., Texas, USA<br /></small></p>',
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<div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-chip.png" alt="CBX8 Antibody ChIP Grade" /></div>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CBX8 (Cat. No. C15410333) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &micro;g of antibody per ChIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for, the tRNA-Ala gene, used as positive control, and for the promoters of the active ACTB and EIF4A2 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP was performed on sheared chromatin from 4 million K562 cells using 2 &micro;g of the Diagenode antibody against CBX8 (Cat. No. C15410333) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human chromosome 1 (fig 2A and B), and along the short arm and two zoomins of chromosome 6 (fig 2C and D). The position of the amplicon used for ChIP-qPCR validation is indicated by an arrow.</small></p>
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<p><small>* Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 &mu;g per ChIP.</small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CBX8 (Cat. No. C15410333) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &micro;g of antibody per ChIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for, the tRNA-Ala gene, used as positive control, and for the promoters of the active ACTB and EIF4A2 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-a.jpg" alt="CBX8 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-b.jpg" alt="CBX8 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-c.jpg" alt="CBX8 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-d.jpg" alt="CBX8 Antibody validated in ChIP-seq" /><br /> E.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-e.jpg" alt="CBX8 Antibody ChIP-seq Grade" /></div>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP was performed on sheared chromatin from 4 million K562 cells using 2 &micro;g of the Diagenode antibody against CBX8 (Cat. No. C15410333) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human chromosome 1 (fig 2A and B), and along the short arm and two zoomins of chromosome 6 (fig 2C and D). The position of the amplicon used for ChIP-qPCR validation is indicated by an arrow.</small></p>
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<div class="row">
<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-wb.png" alt="CBX8 Antibody validated in Western Blot" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CBX8</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX8 (Cat. No. C15410333) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<div class="small-9 columns">
<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CBX8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 &micro;g of the Diagenode antibody against CBX8 (Cat. No. C15410333, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX8 protein was detected by western blot with the CBX8 antibody diluted 1:2,500. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CBX8 (Cat. No. C15410333) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &micro;g of antibody per ChIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for, the tRNA-Ala gene, used as positive control, and for the promoters of the active ACTB and EIF4A2 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP was performed on sheared chromatin from 4 million K562 cells using 2 &micro;g of the Diagenode antibody against CBX8 (Cat. No. C15410333) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human chromosome 1 (fig 2A and B), and along the short arm and two zoomins of chromosome 6 (fig 2C and D). The position of the amplicon used for ChIP-qPCR validation is indicated by an arrow.</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CBX8</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX8 (Cat. No. C15410333) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CBX8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 &micro;g of the Diagenode antibody against CBX8 (Cat. No. C15410333, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX8 protein was detected by western blot with the CBX8 antibody diluted 1:2,500. </small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CBX8 (Cat. No. C15410333) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &micro;g of antibody per ChIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for, the tRNA-Ala gene, used as positive control, and for the promoters of the active ACTB and EIF4A2 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<tr>
<td>ChIP/ChIP-seq *</td>
<td>1-2 &micro;g/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>Western blot</td>
<td>1:1,000</td>
<td>Fig 3</td>
</tr>
<tr>
<td>IP</td>
<td>6 &micro;g per IP</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p></p>
<p><small>* Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 &mu;g per ChIP.</small></p>
<p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>',
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			'name' => 'CBX8 Antibody',
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			'label1' => 'Validation data',
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<div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-chip.png" alt="CBX8 Antibody ChIP Grade" /></div>
<div class="small-8 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CBX8 (Cat. No. C15410333) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &micro;g of antibody per ChIP experiment was analyzed. IgG (2 &micro;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for, the tRNA-Ala gene, used as positive control, and for the promoters of the active ACTB and EIF4A2 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<div class="row">
<div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-a.jpg" alt="CBX8 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-b.jpg" alt="CBX8 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-c.jpg" alt="CBX8 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-d.jpg" alt="CBX8 Antibody validated in ChIP-seq" /><br /> E.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-e.jpg" alt="CBX8 Antibody ChIP-seq Grade" /></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP was performed on sheared chromatin from 4 million K562 cells using 2 &micro;g of the Diagenode antibody against CBX8 (Cat. No. C15410333) as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human chromosome 1 (fig 2A and B), and along the short arm and two zoomins of chromosome 6 (fig 2C and D). The position of the amplicon used for ChIP-qPCR validation is indicated by an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-wb.png" alt="CBX8 Antibody validated in Western Blot" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CBX8</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX8 (Cat. No. C15410333) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-ip.png" alt="CBX8 Antibody validated in Immunoprecipitation" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CBX8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 &micro;g of the Diagenode antibody against CBX8 (Cat. No. C15410333, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX8 protein was detected by western blot with the CBX8 antibody diluted 1:2,500. </small></p>
</div>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
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<p></p>
<p></p>
<p></p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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CBX8 polyclonal antibody DATASHEET Polyclonal antibody raised in rabbit against human CBX8 (chromobox 8), using a synthetic peptide ...	Download
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download

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SDS C15410333 CBX8 Antibody JP ja	Download
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CBX8 polyclonal antibody

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