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<p><small>1 Manufactured by Bethyl Laboratories, Inc., Texas, USA<br /></small></p>',
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<div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-chip.png" alt="CBX8 Antibody ChIP Grade" /></div>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CBX8 (Cat. No. C15410333) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for, the tRNA-Ala gene, used as positive control, and for the promoters of the active ACTB and EIF4A2 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-a.jpg" alt="CBX8 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-b.jpg" alt="CBX8 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-c.jpg" alt="CBX8 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-d.jpg" alt="CBX8 Antibody validated in ChIP-seq" /><br /> E.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-e.jpg" alt="CBX8 Antibody ChIP-seq Grade" /></div>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against CBX8 (Cat. No. C15410333) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human chromosome 1 (fig 2A and B), and along the short arm and two zoomins of chromosome 6 (fig 2C and D). The position of the amplicon used for ChIP-qPCR validation is indicated by an arrow.</small></p>
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<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-wb.png" alt="CBX8 Antibody validated in Western Blot" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CBX8</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX8 (Cat. No. C15410333) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CBX8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against CBX8 (Cat. No. C15410333, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX8 protein was detected by western blot with the CBX8 antibody diluted 1:2,500. </small></p>
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<td>Fig 1, 2</td>
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<p><small>* Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per ChIP.</small></p>
<p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>',
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'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>CBX8 (chromobox 8)</strong>, using a synthetic peptide containing a sequence from the C-terminal part of the protein.</p>
<p><small>1 Manufactured by Bethyl Laboratories, Inc., Texas, USA<br /></small></p>',
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<div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-chip.png" alt="CBX8 Antibody ChIP Grade" /></div>
<div class="small-8 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CBX8 (Cat. No. C15410333) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for, the tRNA-Ala gene, used as positive control, and for the promoters of the active ACTB and EIF4A2 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-a.jpg" alt="CBX8 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-b.jpg" alt="CBX8 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-c.jpg" alt="CBX8 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-d.jpg" alt="CBX8 Antibody validated in ChIP-seq" /><br /> E.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-e.jpg" alt="CBX8 Antibody ChIP-seq Grade" /></div>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against CBX8 (Cat. No. C15410333) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human chromosome 1 (fig 2A and B), and along the short arm and two zoomins of chromosome 6 (fig 2C and D). The position of the amplicon used for ChIP-qPCR validation is indicated by an arrow.</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CBX8</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX8 (Cat. No. C15410333) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CBX8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against CBX8 (Cat. No. C15410333, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX8 protein was detected by western blot with the CBX8 antibody diluted 1:2,500. </small></p>
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<td>ChIP/ChIP-seq *</td>
<td>1-2 µg/ChIP</td>
<td>Fig 1, 2</td>
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<td>Western blot</td>
<td>1:1,000</td>
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<td>Fig 4</td>
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<p><small>* Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per ChIP.</small></p>
<p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>',
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CBX8 (Cat. No. C15410333) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for, the tRNA-Ala gene, used as positive control, and for the promoters of the active ACTB and EIF4A2 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-a.jpg" alt="CBX8 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-b.jpg" alt="CBX8 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-c.jpg" alt="CBX8 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-d.jpg" alt="CBX8 Antibody validated in ChIP-seq" /><br /> E.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-e.jpg" alt="CBX8 Antibody ChIP-seq Grade" /></div>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against CBX8 (Cat. No. C15410333) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human chromosome 1 (fig 2A and B), and along the short arm and two zoomins of chromosome 6 (fig 2C and D). The position of the amplicon used for ChIP-qPCR validation is indicated by an arrow.</small></p>
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<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CBX8</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX8 (Cat. No. C15410333) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-ip.png" alt="CBX8 Antibody validated in Immunoprecipitation" /></div>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CBX8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against CBX8 (Cat. No. C15410333, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX8 protein was detected by western blot with the CBX8 antibody diluted 1:2,500. </small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CBX8 (Cat. No. C15410333) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for, the tRNA-Ala gene, used as positive control, and for the promoters of the active ACTB and EIF4A2 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<div class="row">
<div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-a.jpg" alt="CBX8 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-b.jpg" alt="CBX8 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-c.jpg" alt="CBX8 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-d.jpg" alt="CBX8 Antibody validated in ChIP-seq" /><br /> E.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-e.jpg" alt="CBX8 Antibody ChIP-seq Grade" /></div>
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<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against CBX8 (Cat. No. C15410333) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human chromosome 1 (fig 2A and B), and along the short arm and two zoomins of chromosome 6 (fig 2C and D). The position of the amplicon used for ChIP-qPCR validation is indicated by an arrow.</small></p>
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<div class="row">
<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-wb.png" alt="CBX8 Antibody validated in Western Blot" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CBX8</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX8 (Cat. No. C15410333) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-ip.png" alt="CBX8 Antibody validated in Immunoprecipitation" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CBX8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against CBX8 (Cat. No. C15410333, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX8 protein was detected by western blot with the CBX8 antibody diluted 1:2,500. </small></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CBX8 (Cat. No. C15410333) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for, the tRNA-Ala gene, used as positive control, and for the promoters of the active ACTB and EIF4A2 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against CBX8 (Cat. No. C15410333) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human chromosome 1 (fig 2A and B), and along the short arm and two zoomins of chromosome 6 (fig 2C and D). The position of the amplicon used for ChIP-qPCR validation is indicated by an arrow.</small></p>
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<td>ChIP/ChIP-seq *</td>
<td>1-2 µg/ChIP</td>
<td>Fig 1, 2</td>
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<td>Fig 3</td>
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<p><small>* Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per ChIP.</small></p>
<p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>',
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<div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-chip.png" alt="CBX8 Antibody ChIP Grade" /></div>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CBX8 (Cat. No. C15410333) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for, the tRNA-Ala gene, used as positive control, and for the promoters of the active ACTB and EIF4A2 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-a.jpg" alt="CBX8 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-b.jpg" alt="CBX8 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-c.jpg" alt="CBX8 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-d.jpg" alt="CBX8 Antibody validated in ChIP-seq" /><br /> E.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-e.jpg" alt="CBX8 Antibody ChIP-seq Grade" /></div>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against CBX8 (Cat. No. C15410333) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human chromosome 1 (fig 2A and B), and along the short arm and two zoomins of chromosome 6 (fig 2C and D). The position of the amplicon used for ChIP-qPCR validation is indicated by an arrow.</small></p>
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<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-wb.png" alt="CBX8 Antibody validated in Western Blot" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CBX8</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX8 (Cat. No. C15410333) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-ip.png" alt="CBX8 Antibody validated in Immunoprecipitation" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CBX8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against CBX8 (Cat. No. C15410333, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX8 protein was detected by western blot with the CBX8 antibody diluted 1:2,500. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CBX8 (Cat. No. C15410333) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for, the tRNA-Ala gene, used as positive control, and for the promoters of the active ACTB and EIF4A2 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-a.jpg" alt="CBX8 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-b.jpg" alt="CBX8 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-c.jpg" alt="CBX8 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-d.jpg" alt="CBX8 Antibody validated in ChIP-seq" /><br /> E.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-e.jpg" alt="CBX8 Antibody ChIP-seq Grade" /></div>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against CBX8 (Cat. No. C15410333) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human chromosome 1 (fig 2A and B), and along the short arm and two zoomins of chromosome 6 (fig 2C and D). The position of the amplicon used for ChIP-qPCR validation is indicated by an arrow.</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CBX8</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX8 (Cat. No. C15410333) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CBX8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against CBX8 (Cat. No. C15410333, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX8 protein was detected by western blot with the CBX8 antibody diluted 1:2,500. </small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CBX8 (Cat. No. C15410333) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for, the tRNA-Ala gene, used as positive control, and for the promoters of the active ACTB and EIF4A2 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-a.jpg" alt="CBX8 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-b.jpg" alt="CBX8 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-c.jpg" alt="CBX8 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-d.jpg" alt="CBX8 Antibody validated in ChIP-seq" /><br /> E.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-e.jpg" alt="CBX8 Antibody ChIP-seq Grade" /></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against CBX8 (Cat. No. C15410333) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human chromosome 1 (fig 2A and B), and along the short arm and two zoomins of chromosome 6 (fig 2C and D). The position of the amplicon used for ChIP-qPCR validation is indicated by an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-wb.png" alt="CBX8 Antibody validated in Western Blot" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CBX8</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX8 (Cat. No. C15410333) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-ip.png" alt="CBX8 Antibody validated in Immunoprecipitation" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CBX8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against CBX8 (Cat. No. C15410333, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX8 protein was detected by western blot with the CBX8 antibody diluted 1:2,500. </small></p>
</div>
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'lot' => 'A300-882A2',
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'reactivity' => 'Human: positive. Other species: not tested.',
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<th>Suggested dilution</th>
<th>References</th>
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<td>ChIP/ChIP-seq *</td>
<td>1-2 µg/ChIP</td>
<td>Fig 1, 2</td>
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<tr>
<td>Western blot</td>
<td>1:1,000</td>
<td>Fig 3</td>
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<td>IP</td>
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<td>Fig 4</td>
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<p></p>
<p><small>* Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per ChIP.</small></p>
<p><small><sup>1</sup>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>',
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<div class="small-4 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-chip.png" alt="CBX8 Antibody ChIP Grade" /></div>
<div class="small-8 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against CBX8 (Cat. No. C15410333) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for, the tRNA-Ala gene, used as positive control, and for the promoters of the active ACTB and EIF4A2 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<div class="row">
<div class="small-12 columns">A. <img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-a.jpg" alt="CBX8 Antibody ChIP-seq Grade" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-b.jpg" alt="CBX8 Antibody for ChIP-seq" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-c.jpg" alt="CBX8 Antibody for ChIP-seq assay" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-d.jpg" alt="CBX8 Antibody validated in ChIP-seq" /><br /> E.<img src="https://www.diagenode.com/img/product/antibodies/c15410333-chipseq-e.jpg" alt="CBX8 Antibody ChIP-seq Grade" /></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against CBX8</strong><br /> ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against CBX8 (Cat. No. C15410333) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 3 Mb region of the human chromosome 1 (fig 2A and B), and along the short arm and two zoomins of chromosome 6 (fig 2C and D). The position of the amplicon used for ChIP-qPCR validation is indicated by an arrow.</small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-wb.png" alt="CBX8 Antibody validated in Western Blot" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against CBX8</strong><br /> Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against CBX8 (Cat. No. C15410333) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
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<div class="row">
<div class="small-3 columns"><img src="https://www.diagenode.com/img/product/antibodies/c15410333-ip.png" alt="CBX8 Antibody validated in Immunoprecipitation" /></div>
<div class="small-9 columns">
<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against CBX8</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against CBX8 (Cat. No. C15410333, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated CBX8 protein was detected by western blot with the CBX8 antibody diluted 1:2,500. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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'description' => '<p><span style="font-weight: 400;">All Diagenode’s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
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<p><span style="font-weight: 400;">Diagenode’s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" /> </div>
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<p></p>
<p></p>
<p></p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode’s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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