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CRBN monoclonal antibody

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Format

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C15200245-10

10 μg

$115.00
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C15200245

50 µg

Monoclonal antibody raised in mouse against the N-terminus of the CRBN (Cereblon). Diagenode Cereblon antibody, in contrast to most antibodies on the market, possesses excellent imunoprecipitating properties (see validation data).

Lot	002
Concentration	2 µg/µl
Species reactivity	Human: positive. Other species: not tested.
Type	Monoclonal
Purity	Protein G purified monoclonal antibody.
Host	Mouse
Storage Conditions	Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage Buffer	TBS containing 0.05 % Na-azide.
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
Western blotting	1:500 - :1,000	Fig 1
IP	1:200	Fig 2

Validation data

Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against CRBN
Western blot was performed on 20 µg protein extracts from wild type MOLT4 cells (lane 1) or MOLT4-CRBN-/- cells (lane 2) using the Diagenode antibody against CRBN (cat. No. C15200245) diluted 1:500 in PBS-Tween containing 0.5% NFDM. The marker is shown on the left, position of the CRBN protein is indicated on the right.

Figure 2. IP using the Diagenode monoclonal antibody directed against CRBN
IP was performed on whole cell extracts (400 µg) from either MOLT4 wildtype (lane 1, 3) or MOLT4 CRBN-/- cells (lane 2, 4) using the Diagenode antibody against CRBN (cat. No. C15200245). The immunoprecipitated proteins were subsequently analysed by Western blot with the antibody as described above. Lane 3 and 4 show the result of the IP, the input (20 µg) is shown lane 1 and 2.
Target Description
CRBN (UniProt/Swiss-Prot entry Q96SW2) is a cytoplasmic protease that is related to the Lon protease protein family. It mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2. CRBN is associated with a calcium channel membrane protein and is thought to play a role in brain development and in memory and learning by regulating the assembly and neuronal surface expression of largeconductance calcium-activated potassium channels brain through its interaction with KCNT1. Mutations in the CRBN gene are associated with autosomal recessive nonsyndromic cognitive disability.

資料
CRBN monoclonal antibody datasheet DATASHEET
Monoclonal antibody raised in mouse against the N-terminus of the CRBN (Cereblon). Download

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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against CRBN</strong><br /> Western blot was performed on 20 &micro;g protein extracts from wild type MOLT4 cells (lane 1) or MOLT4-CRBN-/- cells (lane 2) using the Diagenode antibody against CRBN (cat. No. C15200245) diluted 1:500 in PBS-Tween containing 0.5% NFDM. The marker is shown on the left, position of the CRBN protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. IP using the Diagenode monoclonal antibody directed against CRBN</strong><br />IP was performed on whole cell extracts (400 &micro;g) from either MOLT4 wildtype (lane 1, 3) or MOLT4 CRBN-/- cells (lane 2, 4) using the Diagenode antibody against CRBN (cat. No. C15200245). The immunoprecipitated proteins were subsequently analysed by Western blot with the antibody as described above. Lane 3 and 4 show the result of the IP, the input (20 &micro;g) is shown lane 1 and 2.</small></p>
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<div class="small-4 columns">
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against CRBN</strong><br /> Western blot was performed on 20 &micro;g protein extracts from wild type MOLT4 cells (lane 1) or MOLT4-CRBN-/- cells (lane 2) using the Diagenode antibody against CRBN (cat. No. C15200245) diluted 1:500 in PBS-Tween containing 0.5% NFDM. The marker is shown on the left, position of the CRBN protein is indicated on the right.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15200245-IP.png" alt="CRBN Antibody validated in Immunoprecipitation" /></p>
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<p><small><strong>Figure 2. IP using the Diagenode monoclonal antibody directed against CRBN</strong><br />IP was performed on whole cell extracts (400 &micro;g) from either MOLT4 wildtype (lane 1, 3) or MOLT4 CRBN-/- cells (lane 2, 4) using the Diagenode antibody against CRBN (cat. No. C15200245). The immunoprecipitated proteins were subsequently analysed by Western blot with the antibody as described above. Lane 3 and 4 show the result of the IP, the input (20 &micro;g) is shown lane 1 and 2.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against CRBN</strong><br /> Western blot was performed on 20 &micro;g protein extracts from wild type MOLT4 cells (lane 1) or MOLT4-CRBN-/- cells (lane 2) using the Diagenode antibody against CRBN (cat. No. C15200245) diluted 1:500 in PBS-Tween containing 0.5% NFDM. The marker is shown on the left, position of the CRBN protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. IP using the Diagenode monoclonal antibody directed against CRBN</strong><br />IP was performed on whole cell extracts (400 &micro;g) from either MOLT4 wildtype (lane 1, 3) or MOLT4 CRBN-/- cells (lane 2, 4) using the Diagenode antibody against CRBN (cat. No. C15200245). The immunoprecipitated proteins were subsequently analysed by Western blot with the antibody as described above. Lane 3 and 4 show the result of the IP, the input (20 &micro;g) is shown lane 1 and 2.</small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
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<td>Fig 1</td>
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<td>Fig 2</td>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against CRBN</strong><br /> Western blot was performed on 20 &micro;g protein extracts from wild type MOLT4 cells (lane 1) or MOLT4-CRBN-/- cells (lane 2) using the Diagenode antibody against CRBN (cat. No. C15200245) diluted 1:500 in PBS-Tween containing 0.5% NFDM. The marker is shown on the left, position of the CRBN protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. IP using the Diagenode monoclonal antibody directed against CRBN</strong><br />IP was performed on whole cell extracts (400 &micro;g) from either MOLT4 wildtype (lane 1, 3) or MOLT4 CRBN-/- cells (lane 2, 4) using the Diagenode antibody against CRBN (cat. No. C15200245). The immunoprecipitated proteins were subsequently analysed by Western blot with the antibody as described above. Lane 3 and 4 show the result of the IP, the input (20 &micro;g) is shown lane 1 and 2.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against CRBN</strong><br /> Western blot was performed on 20 &micro;g protein extracts from wild type MOLT4 cells (lane 1) or MOLT4-CRBN-/- cells (lane 2) using the Diagenode antibody against CRBN (cat. No. C15200245) diluted 1:500 in PBS-Tween containing 0.5% NFDM. The marker is shown on the left, position of the CRBN protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. IP using the Diagenode monoclonal antibody directed against CRBN</strong><br />IP was performed on whole cell extracts (400 &micro;g) from either MOLT4 wildtype (lane 1, 3) or MOLT4 CRBN-/- cells (lane 2, 4) using the Diagenode antibody against CRBN (cat. No. C15200245). The immunoprecipitated proteins were subsequently analysed by Western blot with the antibody as described above. Lane 3 and 4 show the result of the IP, the input (20 &micro;g) is shown lane 1 and 2.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against CRBN</strong><br /> Western blot was performed on 20 &micro;g protein extracts from wild type MOLT4 cells (lane 1) or MOLT4-CRBN-/- cells (lane 2) using the Diagenode antibody against CRBN (cat. No. C15200245) diluted 1:500 in PBS-Tween containing 0.5% NFDM. The marker is shown on the left, position of the CRBN protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. IP using the Diagenode monoclonal antibody directed against CRBN</strong><br />IP was performed on whole cell extracts (400 &micro;g) from either MOLT4 wildtype (lane 1, 3) or MOLT4 CRBN-/- cells (lane 2, 4) using the Diagenode antibody against CRBN (cat. No. C15200245). The immunoprecipitated proteins were subsequently analysed by Western blot with the antibody as described above. Lane 3 and 4 show the result of the IP, the input (20 &micro;g) is shown lane 1 and 2.</small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against CRBN</strong><br /> Western blot was performed on 20 &micro;g protein extracts from wild type MOLT4 cells (lane 1) or MOLT4-CRBN-/- cells (lane 2) using the Diagenode antibody against CRBN (cat. No. C15200245) diluted 1:500 in PBS-Tween containing 0.5% NFDM. The marker is shown on the left, position of the CRBN protein is indicated on the right.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against CRBN</strong><br /> Western blot was performed on 20 &micro;g protein extracts from wild type MOLT4 cells (lane 1) or MOLT4-CRBN-/- cells (lane 2) using the Diagenode antibody against CRBN (cat. No. C15200245) diluted 1:500 in PBS-Tween containing 0.5% NFDM. The marker is shown on the left, position of the CRBN protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. IP using the Diagenode monoclonal antibody directed against CRBN</strong><br />IP was performed on whole cell extracts (400 &micro;g) from either MOLT4 wildtype (lane 1, 3) or MOLT4 CRBN-/- cells (lane 2, 4) using the Diagenode antibody against CRBN (cat. No. C15200245). The immunoprecipitated proteins were subsequently analysed by Western blot with the antibody as described above. Lane 3 and 4 show the result of the IP, the input (20 &micro;g) is shown lane 1 and 2.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against CRBN</strong><br /> Western blot was performed on 20 &micro;g protein extracts from wild type MOLT4 cells (lane 1) or MOLT4-CRBN-/- cells (lane 2) using the Diagenode antibody against CRBN (cat. No. C15200245) diluted 1:500 in PBS-Tween containing 0.5% NFDM. The marker is shown on the left, position of the CRBN protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. IP using the Diagenode monoclonal antibody directed against CRBN</strong><br />IP was performed on whole cell extracts (400 &micro;g) from either MOLT4 wildtype (lane 1, 3) or MOLT4 CRBN-/- cells (lane 2, 4) using the Diagenode antibody against CRBN (cat. No. C15200245). The immunoprecipitated proteins were subsequently analysed by Western blot with the antibody as described above. Lane 3 and 4 show the result of the IP, the input (20 &micro;g) is shown lane 1 and 2.</small></p>
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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against CRBN</strong><br /> Western blot was performed on 20 &micro;g protein extracts from wild type MOLT4 cells (lane 1) or MOLT4-CRBN-/- cells (lane 2) using the Diagenode antibody against CRBN (cat. No. C15200245) diluted 1:500 in PBS-Tween containing 0.5% NFDM. The marker is shown on the left, position of the CRBN protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. IP using the Diagenode monoclonal antibody directed against CRBN</strong><br />IP was performed on whole cell extracts (400 &micro;g) from either MOLT4 wildtype (lane 1, 3) or MOLT4 CRBN-/- cells (lane 2, 4) using the Diagenode antibody against CRBN (cat. No. C15200245). The immunoprecipitated proteins were subsequently analysed by Western blot with the antibody as described above. Lane 3 and 4 show the result of the IP, the input (20 &micro;g) is shown lane 1 and 2.</small></p>
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small><strong>Figure 1. Western blot analysis using the Diagenode monoclonal antibody directed against CRBN</strong><br /> Western blot was performed on 20 &micro;g protein extracts from wild type MOLT4 cells (lane 1) or MOLT4-CRBN-/- cells (lane 2) using the Diagenode antibody against CRBN (cat. No. C15200245) diluted 1:500 in PBS-Tween containing 0.5% NFDM. The marker is shown on the left, position of the CRBN protein is indicated on the right.</small></p>
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<p><small><strong>Figure 2. IP using the Diagenode monoclonal antibody directed against CRBN</strong><br />IP was performed on whole cell extracts (400 &micro;g) from either MOLT4 wildtype (lane 1, 3) or MOLT4 CRBN-/- cells (lane 2, 4) using the Diagenode antibody against CRBN (cat. No. C15200245). The immunoprecipitated proteins were subsequently analysed by Western blot with the antibody as described above. Lane 3 and 4 show the result of the IP, the input (20 &micro;g) is shown lane 1 and 2.</small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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CRBN monoclonal antibody

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