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D-Plex 24 DNBSEQ Barcodes - Set A

Catalog Number

Format

Price

C05030060

24 rxns

$340.00

D-Plex DNBSEQ Barcodes - Set A includes primers with 24 barcodes to enable multiplexing with the D-Plex Small RNA DNBSEQ^TM Kit. They were designed and validated to fit the D-Plex technology for MGI sequencers.

Two sets are available separately:

D-Plex DNBSEQ Barcodes - Set A
D-Plex DNBSEQ Barcodes - Set B

Set A can be used for library multiplexing up to 24. Set A and Set B can be used simultaneously for library multiplexing up to 48.

Read more about the D-Plex technology.

Multiplexing
The D-Plex Small RNA DNBSEQ library construct bears the MGIEasy DNA Barcodes. In case of a multiplexing scenario, it is recommended to follow MGI’s library pooling guidelines that are explained in the D-Plex Small RNA DNBSEQ Barcodes manual.
資料
D-Plex Small RNA DNBSEQ™ Kit for MGI MANUAL
Download
D-Plex 24 DNBSEQ Barcodes MANUAL
Download
Application note: D-Plex Small RNA DNBSEQ™ Kit for MGI APPLICATION NOTE
Download

Safety sheets

D-Plex 24 DNBSEQ Barcodes for MGI - Set A SDS US en	Download
D-Plex 24 DNBSEQ Barcodes for MGI - Set A SDS GB en	Download
D-Plex 24 DNBSEQ Barcodes for MGI - Set A SDS BE fr	Download
D-Plex 24 DNBSEQ Barcodes for MGI - Set A SDS FR fr	Download
D-Plex 24 DNBSEQ Barcodes for MGI - Set A SDS ES es	Download
D-Plex 24 DNBSEQ Barcodes for MGI - Set A SDS DE de	Download
D-Plex 24 DNBSEQ Barcodes for MGI - Set A SDS JP ja	Download
D-Plex 24 DNBSEQ Barcodes for MGI - Set A SDS BE nl	Download

関連商品
- C05030051
  
  D-Plex Small RNA DNBSEQ™ Kit
- C05030061
  
  D-Plex 24 DNBSEQ Barcodes - Set B
- C03040001
  
  MicroChIP DiaPure columns

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<p><span style="font-weight: 400;">D-Plex DNBSEQ</span> <span style="font-weight: 400;">Barcodes - Set A <span>includes primers with&nbsp;</span><span>24</span><span><span>&nbsp;barcodes</span>&nbsp;to enable multiplexing</span><span>&nbsp;with</span>&nbsp;the </span><a href="https://www.diagenode.com/en/p/d-plex-small-RNA-dnbseq-kit-x24">D-Plex Small RNA DNBSEQ<sup>TM</sup> Kit</a><span style="font-weight: 400;">. They were designed and validated to fit the D-Plex technology for MGI sequencers.</span></p>
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<p><span style="font-weight: 400;"><span>Read more about the&nbsp;</span><a href="https://www.diagenode.com/en/categories/Library-preparation-for-RNA-seq">D-Plex technology</a><b>.</b></span></p>',
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<p><span style="font-weight: 400;">D-Plex DNBSEQ</span> <span style="font-weight: 400;">Barcodes - Set A <span>includes primers with&nbsp;</span><span>24</span><span><span>&nbsp;barcodes</span>&nbsp;to enable multiplexing</span><span>&nbsp;with</span>&nbsp;the </span><a href="https://www.diagenode.com/en/p/d-plex-small-RNA-dnbseq-kit-x24">D-Plex Small RNA DNBSEQ<sup>TM</sup> Kit</a><span style="font-weight: 400;">. They were designed and validated to fit the D-Plex technology for MGI sequencers.</span></p>
<p><span style="font-weight: 400;"> Two sets are available separately:</span></p>
<ul>
<li><span style="font-weight: 400;"></span>D-Plex DNBSEQ Barcodes - Set A</li>
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<p><span style="font-weight: 400;"><span>Read more about the&nbsp;</span><a href="https://www.diagenode.com/en/categories/Library-preparation-for-RNA-seq">D-Plex technology</a><b>.</b></span></p>',
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<p></p>
<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/D-Plex-Small-RNA-DNBSEQ.pdf" target="_blank" title="D-Plex Small RNA DNBSEQ user manual"><strong>Download the manual</strong></a></center>
<p></p>
<p>D-Plex Small RNA DNBSEQ&trade; Kit is a tool designed for the study of the small non-coding transcriptome. The kit is using the <a href="https://www.diagenode.com/en/pages/dplex" target="_blank">D-Plex technology</a> to generate double-stranded DNA libraries ready to be used for the DNA single-strand circularization step required for DNBSEQ sequencing on MGI sequencers.</p>
<p>The D-Plex technology&nbsp;utilizes the innovative capture and amplification by&nbsp;tailing and switching, a&nbsp;ligation-free&nbsp;method for RNA library preparation from ultra-low input amounts, down to 10 pg for small RNAs and 100 pg for total RNAs.&nbsp;This innovative solution enables diverse and novel transcripts detection, even from challenging clinical samples such as liquid biopsies.</p>
<p><span>D-Plex Small&nbsp;RNA&nbsp;<span>DNBSEQ&trade;</span> Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time.&nbsp;</span><span>The library preparation takes place in a&nbsp;</span><span>single tube</span><span>, increasing the efficiency tremendously.&nbsp;This ensures high technical&nbsp;reliability and reproducibility<span>.</span></span></p>
<p>D-Plex Small RNA DNBSEQ&trade; Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex DNBSEQ Barcodes were designed and validated to fit the D-Plex technology and are available separately:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-a">C05030060 - D-Plex DNBSEQ Barcodes for MGI - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-b">C05030061 - D-Plex DNBSEQ Barcodes for MGI - Set B</a></li>
</ul>
<p><b><strong>D-Plex is also available for Illumina sequencing, check<span>&nbsp;</span><a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24" target="_blank">here</a>!</strong></b></p>
<div class="row">
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<div class="container">
<div class="row" style="background: rgba(255,255,255,0.1);">
<div class="large-12 columns slick">
<div>
<h3>Diverse and novel transcript detection</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px; display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-transcript.png" alt="small RNA library preparation for Illumina" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p style="text-align: justify;">The D-Plex Small RNA DNBSEQ protocol generates complex RNA libraries deciphering the wide diversity of small non-coding RNA spectrum (including miRNAs, snoRNAs, snRNAs)&nbsp; in human plasma samples.</p>
</center></div>
</div>
<div>
<h3>Ultra-low input performance</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px; display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-ultralow-input.png" alt="Ultra-low input performance" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p style="text-align: justify;">Sequencing data from circulating RNA samples of two input amounts (25 pg and 2.5 ng) were highly correlated (<i>R = 0.99</i>) when compared using Pearson correlation coefficient.</p>
</center></div>
</div>
<div>
<h3>High mapping efficiency</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px; display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-mapping.png" alt="High mapping efficiency" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p style="text-align: justify;"><b></b>The D-Plex Small RNA DNBSEQ kit is compatible with clinical-relevant samples, such as human plasma, and ultra low range of circulating RNA input (down to 10 pg) and exhibits good read mapping of sequencing reads (up to 70% mapping rate).</p>
</center></div>
</div>
<div>
<h3>High quality DNBSEQ sequencing solution</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-dnbseq.png" alt="High quality DNBSEQ sequencing solution" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p><b></b>The D-Plex Small RNA DNBSEQ kit combines our best-in-class RNA library preparation &ndash; D-Plex technology &ndash; with MGI&rsquo;s high-quality, cost-effective, DNA nanoballs &ndash; DNBSEQ &ndash; sequencing solution, creating a unified platform to support high quality small RNA sequencing.</p>
</center></div>
</div>
</div>
</div>
</div>
</div>
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<li><strong>Ultra-low input capability</strong>: down to 10 pg for small RNAs and 100 pg for total RNAs</li>
<li><strong>High library complexity</strong>:<strong> </strong>obtain a complete view of&nbsp;your small RNA transcriptome</li>
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<p><span style="font-weight: 400;">D-Plex DNBSEQ</span><span><span>&nbsp;</span></span><span style="font-weight: 400;">Barcodes - Set B<span>&nbsp;</span><span>includes primers with&nbsp;</span><span>24</span><span><span>&nbsp;barcodes</span>&nbsp;to enable multiplexing</span><span>&nbsp;with</span>&nbsp;the<span>&nbsp;</span></span><a href="https://www.diagenode.com/en/p/d-plex-small-RNA-dnbseq-kit-x24">D-Plex Small RNA DNBSEQ<sup>TM</sup><span>&nbsp;</span>Kit</a><span style="font-weight: 400;">. They were designed and validated to fit the D-Plex technology for MGI sequencers.</span></p>
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<ul>
<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-a">D-Plex DNBSEQ Barcodes - Set A</a></li>
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<p><strong>Figure 1:</strong> Integrative genomics viewer (IGV) visualization of ChIP-seq experiments using 50,000 of K562 cells.</p>
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<p><img src="https://www.diagenode.com/img/product/kits/figure-diapure-igv.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 2:</strong> Integrative genomics viewer (IGV) visualization of CUT&amp;Tag experiments: MicroChIP DiaPure columns vs phenol-chloroform purification using the H3K27me3 antibody.</p>',
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<p>Diagenode&rsquo;s MicroChIP DiaPure columns have been optimized for the purification and elution of very low amounts of DNA. This rapid method has been validated for epigenetic applications like low input ChIP (e.g. using the True MicroChIP kit) and CUT&amp;Tag (e.g. using Diagenode&rsquo;s pA-Tn5), but is also compatible with many other applications.&nbsp; The DNA can be eluted at high concentrations in volumes down to 6 &mu;l and it is suitable for any downstream application (e.g. NGS).</p>
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<p>Successful ChIP-seq results generated on 50,000 of K562 cells using True MicroChIP technology. ChIP has been performed accordingly to True MicroChIP protocol (Diagenode, Cat. No. C01010130), including DNA purification using the MicroChIP DiaPure columns. For the library preparation the MicroPlex Library Preparation Kit (Diagenode, Cat. No. C05010001) has been used. The below figure shows the peaks from ChIP-seq experiments using the following Diagenode antibodies: H3K4me1 (C15410194), H3K9/14ac (C15410200), H3K27ac (C15410196) and H3K36me3 (C15410192).</p>
<p><img src="https://www.diagenode.com/img/product/kits/figure-igv-microchip.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 1:</strong> Integrative genomics viewer (IGV) visualization of ChIP-seq experiments using 50,000 of K562 cells.</p>
<p></p>
<h2 style="text-align: center;"></h2>
<h2 style="text-align: center;">MicroChIP DiaPure columns after CUT&amp;Tag</h2>
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<p><img src="https://www.diagenode.com/img/product/kits/figure-diapure-igv.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 2:</strong> Integrative genomics viewer (IGV) visualization of CUT&amp;Tag experiments: MicroChIP DiaPure columns vs phenol-chloroform purification using the H3K27me3 antibody.</p>',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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			'description' => '<center><img src="https://www.diagenode.com/img/banners/banner-dplex-mgi.png" width="550" /></center>
<p></p>
<p><span style="font-weight: 400;">D-Plex DNBSEQ</span> <span style="font-weight: 400;">Barcodes - Set A <span>includes primers with&nbsp;</span><span>24</span><span><span>&nbsp;barcodes</span>&nbsp;to enable multiplexing</span><span>&nbsp;with</span>&nbsp;the </span><a href="https://www.diagenode.com/en/p/d-plex-small-RNA-dnbseq-kit-x24">D-Plex Small RNA DNBSEQ<sup>TM</sup> Kit</a><span style="font-weight: 400;">. They were designed and validated to fit the D-Plex technology for MGI sequencers.</span></p>
<p><span style="font-weight: 400;"> Two sets are available separately:</span></p>
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<li><span style="font-weight: 400;"></span><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-b">D-Plex DNBSEQ Barcodes - Set B</a></li>
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<p><span style="font-weight: 400;"><span>Read more about the&nbsp;</span><a href="https://www.diagenode.com/en/categories/Library-preparation-for-RNA-seq">D-Plex technology</a><b>.</b></span></p>',
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<p></p>
<p><span style="font-weight: 400;">D-Plex DNBSEQ</span> <span style="font-weight: 400;">Barcodes - Set A <span>includes primers with&nbsp;</span><span>24</span><span><span>&nbsp;barcodes</span>&nbsp;to enable multiplexing</span><span>&nbsp;with</span>&nbsp;the </span><a href="https://www.diagenode.com/en/p/d-plex-small-RNA-dnbseq-kit-x24">D-Plex Small RNA DNBSEQ<sup>TM</sup> Kit</a><span style="font-weight: 400;">. They were designed and validated to fit the D-Plex technology for MGI sequencers.</span></p>
<p><span style="font-weight: 400;"> Two sets are available separately:</span></p>
<ul>
<li><span style="font-weight: 400;"></span>D-Plex DNBSEQ Barcodes - Set A</li>
<li><span style="font-weight: 400;"></span><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-b">D-Plex DNBSEQ Barcodes - Set B</a></li>
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<p><span style="font-weight: 400;">Set A can be used for library multiplexing up to 24.&nbsp;</span><span style="font-weight: 400;">Set A and Set B can be used simultaneously for library multiplexing up to 48.</span></p>
<p><span style="font-weight: 400;"><span>Read more about the&nbsp;</span><a href="https://www.diagenode.com/en/categories/Library-preparation-for-RNA-seq">D-Plex technology</a><b>.</b></span></p>',
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<p></p>
<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/D-Plex-Small-RNA-DNBSEQ.pdf" target="_blank" title="D-Plex Small RNA DNBSEQ user manual"><strong>Download the manual</strong></a></center>
<p></p>
<p>D-Plex Small RNA DNBSEQ&trade; Kit is a tool designed for the study of the small non-coding transcriptome. The kit is using the <a href="https://www.diagenode.com/en/pages/dplex" target="_blank">D-Plex technology</a> to generate double-stranded DNA libraries ready to be used for the DNA single-strand circularization step required for DNBSEQ sequencing on MGI sequencers.</p>
<p>The D-Plex technology&nbsp;utilizes the innovative capture and amplification by&nbsp;tailing and switching, a&nbsp;ligation-free&nbsp;method for RNA library preparation from ultra-low input amounts, down to 10 pg for small RNAs and 100 pg for total RNAs.&nbsp;This innovative solution enables diverse and novel transcripts detection, even from challenging clinical samples such as liquid biopsies.</p>
<p><span>D-Plex Small&nbsp;RNA&nbsp;<span>DNBSEQ&trade;</span> Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time.&nbsp;</span><span>The library preparation takes place in a&nbsp;</span><span>single tube</span><span>, increasing the efficiency tremendously.&nbsp;This ensures high technical&nbsp;reliability and reproducibility<span>.</span></span></p>
<p>D-Plex Small RNA DNBSEQ&trade; Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex DNBSEQ Barcodes were designed and validated to fit the D-Plex technology and are available separately:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-a">C05030060 - D-Plex DNBSEQ Barcodes for MGI - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-b">C05030061 - D-Plex DNBSEQ Barcodes for MGI - Set B</a></li>
</ul>
<p><b><strong>D-Plex is also available for Illumina sequencing, check<span>&nbsp;</span><a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24" target="_blank">here</a>!</strong></b></p>
<div class="row">
<div class="carrousel" style="background-position: center;">
<div class="container">
<div class="row" style="background: rgba(255,255,255,0.1);">
<div class="large-12 columns slick">
<div>
<h3>Diverse and novel transcript detection</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px; display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-transcript.png" alt="small RNA library preparation for Illumina" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p style="text-align: justify;">The D-Plex Small RNA DNBSEQ protocol generates complex RNA libraries deciphering the wide diversity of small non-coding RNA spectrum (including miRNAs, snoRNAs, snRNAs)&nbsp; in human plasma samples.</p>
</center></div>
</div>
<div>
<h3>Ultra-low input performance</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px; display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-ultralow-input.png" alt="Ultra-low input performance" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p style="text-align: justify;">Sequencing data from circulating RNA samples of two input amounts (25 pg and 2.5 ng) were highly correlated (<i>R = 0.99</i>) when compared using Pearson correlation coefficient.</p>
</center></div>
</div>
<div>
<h3>High mapping efficiency</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px; display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-mapping.png" alt="High mapping efficiency" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p style="text-align: justify;"><b></b>The D-Plex Small RNA DNBSEQ kit is compatible with clinical-relevant samples, such as human plasma, and ultra low range of circulating RNA input (down to 10 pg) and exhibits good read mapping of sequencing reads (up to 70% mapping rate).</p>
</center></div>
</div>
<div>
<h3>High quality DNBSEQ sequencing solution</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-dnbseq.png" alt="High quality DNBSEQ sequencing solution" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p><b></b>The D-Plex Small RNA DNBSEQ kit combines our best-in-class RNA library preparation &ndash; D-Plex technology &ndash; with MGI&rsquo;s high-quality, cost-effective, DNA nanoballs &ndash; DNBSEQ &ndash; sequencing solution, creating a unified platform to support high quality small RNA sequencing.</p>
</center></div>
</div>
</div>
</div>
</div>
</div>
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<li><strong>Ultra-low input capability</strong>: down to 10 pg for small RNAs and 100 pg for total RNAs</li>
<li><strong>High library complexity</strong>:<strong> </strong>obtain a complete view of&nbsp;your small RNA transcriptome</li>
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<p><span style="font-weight: 400;">D-Plex DNBSEQ</span><span><span>&nbsp;</span></span><span style="font-weight: 400;">Barcodes - Set B<span>&nbsp;</span><span>includes primers with&nbsp;</span><span>24</span><span><span>&nbsp;barcodes</span>&nbsp;to enable multiplexing</span><span>&nbsp;with</span>&nbsp;the<span>&nbsp;</span></span><a href="https://www.diagenode.com/en/p/d-plex-small-RNA-dnbseq-kit-x24">D-Plex Small RNA DNBSEQ<sup>TM</sup><span>&nbsp;</span>Kit</a><span style="font-weight: 400;">. They were designed and validated to fit the D-Plex technology for MGI sequencers.</span></p>
<p>Two sets are available separately:</p>
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<p>Set A can be used for library multiplexing up to 24. Set A and Set B can be used simultaneously for library multiplexing up to 48.</p>
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			'meta_description' => 'Compatible with D-Plex Small RNA DNBSEQ kit - Barcodes for MGI sequencing - Multiplexing up to 48 samples - Suitable for ultra-low input (100 pg total RNA)',
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<p>Diagenode&rsquo;s MicroChIP DiaPure columns have been optimized for the purification and elution of very low amounts of DNA. This rapid method has been validated for epigenetic applications like low input ChIP (e.g. using the True MicroChIP kit) and CUT&amp;Tag (e.g. using Diagenode&rsquo;s pA-Tn5), but is also compatible with many other applications.&nbsp; The DNA can be eluted at high concentrations in volumes down to 6 &mu;l and it is suitable for any downstream application (e.g. NGS).</p>
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<p>Successful ChIP-seq results generated on 50,000 of K562 cells using True MicroChIP technology. ChIP has been performed accordingly to True MicroChIP protocol (Diagenode, Cat. No. C01010130), including DNA purification using the MicroChIP DiaPure columns. For the library preparation the MicroPlex Library Preparation Kit (Diagenode, Cat. No. C05010001) has been used. The below figure shows the peaks from ChIP-seq experiments using the following Diagenode antibodies: H3K4me1 (C15410194), H3K9/14ac (C15410200), H3K27ac (C15410196) and H3K36me3 (C15410192).</p>
<p><img src="https://www.diagenode.com/img/product/kits/figure-igv-microchip.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 1:</strong> Integrative genomics viewer (IGV) visualization of ChIP-seq experiments using 50,000 of K562 cells.</p>
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<h2 style="text-align: center;"></h2>
<h2 style="text-align: center;">MicroChIP DiaPure columns after CUT&amp;Tag</h2>
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<p><img src="https://www.diagenode.com/img/product/kits/figure-diapure-igv.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 2:</strong> Integrative genomics viewer (IGV) visualization of CUT&amp;Tag experiments: MicroChIP DiaPure columns vs phenol-chloroform purification using the H3K27me3 antibody.</p>',
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<p><img src="https://www.diagenode.com/img/product/kits/figure-igv-microchip.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<h2 style="text-align: center;"></h2>
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<p><img src="https://www.diagenode.com/img/product/kits/figure-diapure-igv.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 2:</strong> Integrative genomics viewer (IGV) visualization of CUT&amp;Tag experiments: MicroChIP DiaPure columns vs phenol-chloroform purification using the H3K27me3 antibody.</p>',
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
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<p></p>
<p><span style="font-weight: 400;">D-Plex DNBSEQ</span> <span style="font-weight: 400;">Barcodes - Set A <span>includes primers with&nbsp;</span><span>24</span><span><span>&nbsp;barcodes</span>&nbsp;to enable multiplexing</span><span>&nbsp;with</span>&nbsp;the </span><a href="https://www.diagenode.com/en/p/d-plex-small-RNA-dnbseq-kit-x24">D-Plex Small RNA DNBSEQ<sup>TM</sup> Kit</a><span style="font-weight: 400;">. They were designed and validated to fit the D-Plex technology for MGI sequencers.</span></p>
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<p><span style="font-weight: 400;">Set A can be used for library multiplexing up to 24.&nbsp;</span><span style="font-weight: 400;">Set A and Set B can be used simultaneously for library multiplexing up to 48.</span></p>
<p><span style="font-weight: 400;"><span>Read more about the&nbsp;</span><a href="https://www.diagenode.com/en/categories/Library-preparation-for-RNA-seq">D-Plex technology</a><b>.</b></span></p>',
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		'name' => 'D-Plex 24 DNBSEQ Barcodes - Set A',
		'description' => '<center><img src="https://www.diagenode.com/img/banners/banner-dplex-mgi.png" width="550" /></center>
<p></p>
<p><span style="font-weight: 400;">D-Plex DNBSEQ</span> <span style="font-weight: 400;">Barcodes - Set A <span>includes primers with&nbsp;</span><span>24</span><span><span>&nbsp;barcodes</span>&nbsp;to enable multiplexing</span><span>&nbsp;with</span>&nbsp;the </span><a href="https://www.diagenode.com/en/p/d-plex-small-RNA-dnbseq-kit-x24">D-Plex Small RNA DNBSEQ<sup>TM</sup> Kit</a><span style="font-weight: 400;">. They were designed and validated to fit the D-Plex technology for MGI sequencers.</span></p>
<p><span style="font-weight: 400;"> Two sets are available separately:</span></p>
<ul>
<li><span style="font-weight: 400;"></span>D-Plex DNBSEQ Barcodes - Set A</li>
<li><span style="font-weight: 400;"></span><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-b">D-Plex DNBSEQ Barcodes - Set B</a></li>
</ul>
<p><span style="font-weight: 400;">Set A can be used for library multiplexing up to 24.&nbsp;</span><span style="font-weight: 400;">Set A and Set B can be used simultaneously for library multiplexing up to 48.</span></p>
<p><span style="font-weight: 400;"><span>Read more about the&nbsp;</span><a href="https://www.diagenode.com/en/categories/Library-preparation-for-RNA-seq">D-Plex technology</a><b>.</b></span></p>',
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<p></p>
<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/D-Plex-Small-RNA-DNBSEQ.pdf" target="_blank" title="D-Plex Small RNA DNBSEQ user manual"><strong>Download the manual</strong></a></center>
<p></p>
<p>D-Plex Small RNA DNBSEQ&trade; Kit is a tool designed for the study of the small non-coding transcriptome. The kit is using the <a href="https://www.diagenode.com/en/pages/dplex" target="_blank">D-Plex technology</a> to generate double-stranded DNA libraries ready to be used for the DNA single-strand circularization step required for DNBSEQ sequencing on MGI sequencers.</p>
<p>The D-Plex technology&nbsp;utilizes the innovative capture and amplification by&nbsp;tailing and switching, a&nbsp;ligation-free&nbsp;method for RNA library preparation from ultra-low input amounts, down to 10 pg for small RNAs and 100 pg for total RNAs.&nbsp;This innovative solution enables diverse and novel transcripts detection, even from challenging clinical samples such as liquid biopsies.</p>
<p><span>D-Plex Small&nbsp;RNA&nbsp;<span>DNBSEQ&trade;</span> Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time.&nbsp;</span><span>The library preparation takes place in a&nbsp;</span><span>single tube</span><span>, increasing the efficiency tremendously.&nbsp;This ensures high technical&nbsp;reliability and reproducibility<span>.</span></span></p>
<p>D-Plex Small RNA DNBSEQ&trade; Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex DNBSEQ Barcodes were designed and validated to fit the D-Plex technology and are available separately:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-a">C05030060 - D-Plex DNBSEQ Barcodes for MGI - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-b">C05030061 - D-Plex DNBSEQ Barcodes for MGI - Set B</a></li>
</ul>
<p><b><strong>D-Plex is also available for Illumina sequencing, check<span>&nbsp;</span><a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24" target="_blank">here</a>!</strong></b></p>
<div class="row">
<div class="carrousel" style="background-position: center;">
<div class="container">
<div class="row" style="background: rgba(255,255,255,0.1);">
<div class="large-12 columns slick">
<div>
<h3>Diverse and novel transcript detection</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px; display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-transcript.png" alt="small RNA library preparation for Illumina" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p style="text-align: justify;">The D-Plex Small RNA DNBSEQ protocol generates complex RNA libraries deciphering the wide diversity of small non-coding RNA spectrum (including miRNAs, snoRNAs, snRNAs)&nbsp; in human plasma samples.</p>
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<h3>Ultra-low input performance</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px; display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-ultralow-input.png" alt="Ultra-low input performance" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p style="text-align: justify;">Sequencing data from circulating RNA samples of two input amounts (25 pg and 2.5 ng) were highly correlated (<i>R = 0.99</i>) when compared using Pearson correlation coefficient.</p>
</center></div>
</div>
<div>
<h3>High mapping efficiency</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px; display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-mapping.png" alt="High mapping efficiency" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p style="text-align: justify;"><b></b>The D-Plex Small RNA DNBSEQ kit is compatible with clinical-relevant samples, such as human plasma, and ultra low range of circulating RNA input (down to 10 pg) and exhibits good read mapping of sequencing reads (up to 70% mapping rate).</p>
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<div>
<h3>High quality DNBSEQ sequencing solution</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-dnbseq.png" alt="High quality DNBSEQ sequencing solution" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p><b></b>The D-Plex Small RNA DNBSEQ kit combines our best-in-class RNA library preparation &ndash; D-Plex technology &ndash; with MGI&rsquo;s high-quality, cost-effective, DNA nanoballs &ndash; DNBSEQ &ndash; sequencing solution, creating a unified platform to support high quality small RNA sequencing.</p>
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<p><span style="font-weight: 400;">D-Plex DNBSEQ</span><span><span>&nbsp;</span></span><span style="font-weight: 400;">Barcodes - Set B<span>&nbsp;</span><span>includes primers with&nbsp;</span><span>24</span><span><span>&nbsp;barcodes</span>&nbsp;to enable multiplexing</span><span>&nbsp;with</span>&nbsp;the<span>&nbsp;</span></span><a href="https://www.diagenode.com/en/p/d-plex-small-RNA-dnbseq-kit-x24">D-Plex Small RNA DNBSEQ<sup>TM</sup><span>&nbsp;</span>Kit</a><span style="font-weight: 400;">. They were designed and validated to fit the D-Plex technology for MGI sequencers.</span></p>
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<p>Successful ChIP-seq results generated on 50,000 of K562 cells using True MicroChIP technology. ChIP has been performed accordingly to True MicroChIP protocol (Diagenode, Cat. No. C01010130), including DNA purification using the MicroChIP DiaPure columns. For the library preparation the MicroPlex Library Preparation Kit (Diagenode, Cat. No. C05010001) has been used. The below figure shows the peaks from ChIP-seq experiments using the following Diagenode antibodies: H3K4me1 (C15410194), H3K9/14ac (C15410200), H3K27ac (C15410196) and H3K36me3 (C15410192).</p>
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<p><strong>Figure 1:</strong> Integrative genomics viewer (IGV) visualization of ChIP-seq experiments using 50,000 of K562 cells.</p>
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<h2 style="text-align: center;"></h2>
<h2 style="text-align: center;">MicroChIP DiaPure columns after CUT&amp;Tag</h2>
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<p><strong>Figure 2:</strong> Integrative genomics viewer (IGV) visualization of CUT&amp;Tag experiments: MicroChIP DiaPure columns vs phenol-chloroform purification using the H3K27me3 antibody.</p>',
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        <div class="small-12 columns">
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        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="d-plex-small-RNA-dnbseq-kit-x24" data-reveal-id="cartModal-3170" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
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        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">D-Plex Small RNA DNBSEQ™ Kit </h6>
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    </div>
</li>
<li>
    <div class="row">
        <div class="small-12 columns">
                         <a href="/jp/p/d-plex-24-dnbseq-barcodes-set-b"><img src="/img/product/kits/kit-icon.png" alt="Kit icon" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
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            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
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                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-3169" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/jp/carts/add/3169" id="CartAdd/3169Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="3169" id="CartProductId"/>
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        <p><strong><input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/></strong>D-Plex DNBSEQ Barcodes for MGI Sequencing - Set B個カートに追加。</p>

        <div class="row">
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('D-Plex DNBSEQ Barcodes for MGI Sequencing - Set B',
                    'C05030061',
                     '340',
                       $('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">お会計</button>            </div>
            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('D-Plex DNBSEQ Barcodes for MGI Sequencing - Set B',
                    'C05030061',
                    '340',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="d-plex-24-dnbseq-barcodes-set-b" data-reveal-id="cartModal-3169" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
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        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">D-Plex 24 DNBSEQ Barcodes - Set B</h6>
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    </div>
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<li>
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        <div class="small-12 columns">
                         <a href="/jp/p/microchip-diapure-columns-50-rxns"><img src="/img/grey-logo.jpg" alt="default alt" class="th"/></a>        </div>



        <div class="small-12 columns">
            <div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
                <span class="success label" style="">C03040001</span>
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            <div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
                <!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
                <!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1923" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/jp/carts/add/1923" id="CartAdd/1923Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1923" id="CartProductId"/>
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            <div class="small-6 medium-6 large-6 columns">
                <button class="alert small button expand" onclick="$(this).addToCart('MicroChIP DiaPure columns',
                    'C03040001',
                    '125',
                       $('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">お買い物を続ける</button>            </div>
        </div>
    </div>
</div>

</form><a class="close-reveal-modal" aria-label="Close">&#215;</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="microchip-diapure-columns-50-rxns" data-reveal-id="cartModal-1923" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
            </div>

        </div>
        <div class="small-12 columns" >
            <h6 style="height:60px">MicroChIP DiaPure columns</h6>
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    </div>
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<p>Diagenode&rsquo;s MicroChIP DiaPure columns have been optimized for the purification and elution of very low amounts of DNA. This rapid method has been validated for epigenetic applications like low input ChIP (e.g. using the True MicroChIP kit) and CUT&amp;Tag (e.g. using Diagenode&rsquo;s pA-Tn5), but is also compatible with many other applications.&nbsp; The DNA can be eluted at high concentrations in volumes down to 6 &mu;l and it is suitable for any downstream application (e.g. NGS).</p>
<p>Benefits of the MicroChIP DiaPure columns:</p>
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<li>Optimized for the purification of very low DNA amounts</li>
<li>Fast and easy protocol</li>
<li>Non-toxic</li>
<li>Validated for ChIP and Cut&amp;Tag</li>
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	'info1' => '<h2 style="text-align: center;">MicroChIP DiaPure columns after ChIP</h2>
<p>Successful ChIP-seq results generated on 50,000 of K562 cells using True MicroChIP technology. ChIP has been performed accordingly to True MicroChIP protocol (Diagenode, Cat. No. C01010130), including DNA purification using the MicroChIP DiaPure columns. For the library preparation the MicroPlex Library Preparation Kit (Diagenode, Cat. No. C05010001) has been used. The below figure shows the peaks from ChIP-seq experiments using the following Diagenode antibodies: H3K4me1 (C15410194), H3K9/14ac (C15410200), H3K27ac (C15410196) and H3K36me3 (C15410192).</p>
<p><img src="https://www.diagenode.com/img/product/kits/figure-igv-microchip.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 1:</strong> Integrative genomics viewer (IGV) visualization of ChIP-seq experiments using 50,000 of K562 cells.</p>
<p></p>
<h2 style="text-align: center;"></h2>
<h2 style="text-align: center;">MicroChIP DiaPure columns after CUT&amp;Tag</h2>
<p>Successful CUT&amp;Tag results showing a low background with high region-specific enrichment has been generated using 50.000 of K562 cells, 1 &micro;g of H3K27me3 antibody (Diagenode, Cat. No. C15410069) and proteinA-Tn5 (1:250) (Diagenode, Cat. No. C01070001). 1 &micro;g of IgG (Diagenode, Cat. No. C15410206) was used as negative control. Samples were purified using the MicroChIP DiaPure columns or phenol-chloroform purification. The below figure presenst the comparison of two purification methods.</p>
<p><img src="https://www.diagenode.com/img/product/kits/figure-diapure-igv.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 2:</strong> Integrative genomics viewer (IGV) visualization of CUT&amp;Tag experiments: MicroChIP DiaPure columns vs phenol-chloroform purification using the H3K27me3 antibody.</p>',
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	'info2' => '',
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	'format' => '50 rxns',
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	'type' => 'REF',
	'search_order' => '04-undefined',
	'price_EUR' => '125',
	'price_USD' => '125',
	'price_GBP' => '115',
	'price_JPY' => '19580',
	'price_CNY' => '',
	'price_AUD' => '312',
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	'featured' => false,
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	'online' => true,
	'master' => true,
	'last_datasheet_update' => '0000-00-00',
	'slug' => 'microchip-diapure-columns-50-rxns',
	'meta_title' => 'MicroChIP DiaPure columns',
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	'meta_description' => 'MicroChIP DiaPure columns',
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$rrbs_service = array(
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	(int) 1 => (int) 1895
)
$chipseq_service = array(
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	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ''
$country_code = 'US'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$document = array(
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	'slug' => 'd-plex-mgi-application-note',
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	'url' => 'files/SDS/D-Plex/SDS-C05030060-D-Plex_24_DNBSEQ_Barcodes_for_MGI_-_Set_A-BE-nl-GHS_1_0.pdf',
	'countries' => 'BE',
	'modified' => '2021-09-30 12:51:16',
	'created' => '2021-09-30 12:51:16',
	'ProductsSafetySheet' => array(
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		'product_id' => '3157',
		'safety_sheet_id' => '1810'
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'jp',
	'meta_keywords' => '',
	'meta_description' => '',
	'meta_title' => 'D-Plex 24 DNBSEQ Barcodes - Set A',
	'product' => array(
		'Product' => array(
			'id' => '3157',
			'antibody_id' => null,
			'name' => 'D-Plex 24 DNBSEQ Barcodes - Set A',
			'description' => '<center><img src="https://www.diagenode.com/img/banners/banner-dplex-mgi.png" width="550" /></center>
<p></p>
<p><span style="font-weight: 400;">D-Plex DNBSEQ</span> <span style="font-weight: 400;">Barcodes - Set A <span>includes primers with&nbsp;</span><span>24</span><span><span>&nbsp;barcodes</span>&nbsp;to enable multiplexing</span><span>&nbsp;with</span>&nbsp;the </span><a href="https://www.diagenode.com/en/p/d-plex-small-RNA-dnbseq-kit-x24">D-Plex Small RNA DNBSEQ<sup>TM</sup> Kit</a><span style="font-weight: 400;">. They were designed and validated to fit the D-Plex technology for MGI sequencers.</span></p>
<p><span style="font-weight: 400;"> Two sets are available separately:</span></p>
<ul>
<li><span style="font-weight: 400;"></span>D-Plex DNBSEQ Barcodes - Set A</li>
<li><span style="font-weight: 400;"></span><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-b">D-Plex DNBSEQ Barcodes - Set B</a></li>
</ul>
<p><span style="font-weight: 400;">Set A can be used for library multiplexing up to 24.&nbsp;</span><span style="font-weight: 400;">Set A and Set B can be used simultaneously for library multiplexing up to 48.</span></p>
<p><span style="font-weight: 400;"><span>Read more about the&nbsp;</span><a href="https://www.diagenode.com/en/categories/Library-preparation-for-RNA-seq">D-Plex technology</a><b>.</b></span></p>',
			'label1' => 'Multiplexing',
			'info1' => '<p><span>The D-Plex Small RNA DNBSEQ library construct bears the MGIEasy&nbsp;</span><span>DNA Barcodes. In case of a multiplexing scenario, it is recommended to follow MGI&rsquo;s library pooling guidelines that are explained in the D-Plex Small RNA DNBSEQ Barcodes manual.</span></p>',
			'label2' => '',
			'info2' => '',
			'label3' => '',
			'info3' => '',
			'format' => '24 rxns',
			'catalog_number' => 'C05030060',
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			'sf_code' => 'C05030060-',
			'type' => 'FRE',
			'search_order' => '',
			'price_EUR' => '340',
			'price_USD' => '340',
			'price_GBP' => '300',
			'price_JPY' => '53260',
			'price_CNY' => '',
			'price_AUD' => '850',
			'country' => 'ALL',
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			'featured' => true,
			'no_promo' => false,
			'online' => true,
			'master' => true,
			'last_datasheet_update' => '',
			'slug' => 'd-plex-24-dnbseq-barcodes-set-a',
			'meta_title' => 'D-Plex 24 DNBSEQ Barcodes - Set A',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2021-04-22 12:21:09',
			'created' => '2020-12-08 12:25:52',
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			'precautions' => null,
			'uniprot_acc' => null,
			'slug' => null,
			'meta_keywords' => null,
			'meta_description' => null,
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			'select_label' => null
		),
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		'Group' => array(),
		'Related' => array(
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		),
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			(int) 2 => array(
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		'Feature' => array(),
		'Image' => array(
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		),
		'Promotion' => array(),
		'Protocol' => array(),
		'Publication' => array(),
		'Testimonial' => array(),
		'Area' => array(),
		'SafetySheet' => array(
			(int) 0 => array(
				[maximum depth reached]
			),
			(int) 1 => array(
				[maximum depth reached]
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			(int) 2 => array(
				[maximum depth reached]
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			(int) 3 => array(
				[maximum depth reached]
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			(int) 4 => array(
				[maximum depth reached]
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	)
)
$language = 'jp'
$meta_keywords = ''
$meta_description = ''
$meta_title = 'D-Plex 24 DNBSEQ Barcodes - Set A'
$product = array(
	'Product' => array(
		'id' => '3157',
		'antibody_id' => null,
		'name' => 'D-Plex 24 DNBSEQ Barcodes - Set A',
		'description' => '<center><img src="https://www.diagenode.com/img/banners/banner-dplex-mgi.png" width="550" /></center>
<p></p>
<p><span style="font-weight: 400;">D-Plex DNBSEQ</span> <span style="font-weight: 400;">Barcodes - Set A <span>includes primers with&nbsp;</span><span>24</span><span><span>&nbsp;barcodes</span>&nbsp;to enable multiplexing</span><span>&nbsp;with</span>&nbsp;the </span><a href="https://www.diagenode.com/en/p/d-plex-small-RNA-dnbseq-kit-x24">D-Plex Small RNA DNBSEQ<sup>TM</sup> Kit</a><span style="font-weight: 400;">. They were designed and validated to fit the D-Plex technology for MGI sequencers.</span></p>
<p><span style="font-weight: 400;"> Two sets are available separately:</span></p>
<ul>
<li><span style="font-weight: 400;"></span>D-Plex DNBSEQ Barcodes - Set A</li>
<li><span style="font-weight: 400;"></span><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-b">D-Plex DNBSEQ Barcodes - Set B</a></li>
</ul>
<p><span style="font-weight: 400;">Set A can be used for library multiplexing up to 24.&nbsp;</span><span style="font-weight: 400;">Set A and Set B can be used simultaneously for library multiplexing up to 48.</span></p>
<p><span style="font-weight: 400;"><span>Read more about the&nbsp;</span><a href="https://www.diagenode.com/en/categories/Library-preparation-for-RNA-seq">D-Plex technology</a><b>.</b></span></p>',
		'label1' => 'Multiplexing',
		'info1' => '<p><span>The D-Plex Small RNA DNBSEQ library construct bears the MGIEasy&nbsp;</span><span>DNA Barcodes. In case of a multiplexing scenario, it is recommended to follow MGI&rsquo;s library pooling guidelines that are explained in the D-Plex Small RNA DNBSEQ Barcodes manual.</span></p>',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '24 rxns',
		'catalog_number' => 'C05030060',
		'old_catalog_number' => '',
		'sf_code' => 'C05030060-',
		'type' => 'FRE',
		'search_order' => '',
		'price_EUR' => '340',
		'price_USD' => '340',
		'price_GBP' => '300',
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			'name' => 'D-Plex Small RNA DNBSEQ™ Kit for MGI Sequencing',
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<p></p>
<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/D-Plex-Small-RNA-DNBSEQ.pdf" target="_blank" title="D-Plex Small RNA DNBSEQ user manual"><strong>Download the manual</strong></a></center>
<p></p>
<p>D-Plex Small RNA DNBSEQ&trade; Kit is a tool designed for the study of the small non-coding transcriptome. The kit is using the <a href="https://www.diagenode.com/en/pages/dplex" target="_blank">D-Plex technology</a> to generate double-stranded DNA libraries ready to be used for the DNA single-strand circularization step required for DNBSEQ sequencing on MGI sequencers.</p>
<p>The D-Plex technology&nbsp;utilizes the innovative capture and amplification by&nbsp;tailing and switching, a&nbsp;ligation-free&nbsp;method for RNA library preparation from ultra-low input amounts, down to 10 pg for small RNAs and 100 pg for total RNAs.&nbsp;This innovative solution enables diverse and novel transcripts detection, even from challenging clinical samples such as liquid biopsies.</p>
<p><span>D-Plex Small&nbsp;RNA&nbsp;<span>DNBSEQ&trade;</span> Kit offers a time saving protocol that can be completed within 5 hours and requires minimal hands-on time.&nbsp;</span><span>The library preparation takes place in a&nbsp;</span><span>single tube</span><span>, increasing the efficiency tremendously.&nbsp;This ensures high technical&nbsp;reliability and reproducibility<span>.</span></span></p>
<p>D-Plex Small RNA DNBSEQ&trade; Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex DNBSEQ Barcodes were designed and validated to fit the D-Plex technology and are available separately:</p>
<ul>
<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-a">C05030060 - D-Plex DNBSEQ Barcodes for MGI - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-b">C05030061 - D-Plex DNBSEQ Barcodes for MGI - Set B</a></li>
</ul>
<p><b><strong>D-Plex is also available for Illumina sequencing, check<span>&nbsp;</span><a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24" target="_blank">here</a>!</strong></b></p>
<div class="row">
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<div class="container">
<div class="row" style="background: rgba(255,255,255,0.1);">
<div class="large-12 columns slick">
<div>
<h3>Diverse and novel transcript detection</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px; display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-transcript.png" alt="small RNA library preparation for Illumina" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p style="text-align: justify;">The D-Plex Small RNA DNBSEQ protocol generates complex RNA libraries deciphering the wide diversity of small non-coding RNA spectrum (including miRNAs, snoRNAs, snRNAs)&nbsp; in human plasma samples.</p>
</center></div>
</div>
<div>
<h3>Ultra-low input performance</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px; display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-ultralow-input.png" alt="Ultra-low input performance" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p style="text-align: justify;">Sequencing data from circulating RNA samples of two input amounts (25 pg and 2.5 ng) were highly correlated (<i>R = 0.99</i>) when compared using Pearson correlation coefficient.</p>
</center></div>
</div>
<div>
<h3>High mapping efficiency</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px; display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-mapping.png" alt="High mapping efficiency" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p style="text-align: justify;"><b></b>The D-Plex Small RNA DNBSEQ kit is compatible with clinical-relevant samples, such as human plasma, and ultra low range of circulating RNA input (down to 10 pg) and exhibits good read mapping of sequencing reads (up to 70% mapping rate).</p>
</center></div>
</div>
<div>
<h3>High quality DNBSEQ sequencing solution</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-dnbseq.png" alt="High quality DNBSEQ sequencing solution" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p><b></b>The D-Plex Small RNA DNBSEQ kit combines our best-in-class RNA library preparation &ndash; D-Plex technology &ndash; with MGI&rsquo;s high-quality, cost-effective, DNA nanoballs &ndash; DNBSEQ &ndash; sequencing solution, creating a unified platform to support high quality small RNA sequencing.</p>
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</div>
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<li><strong>Ultra-low input capability</strong>: down to 10 pg for small RNAs and 100 pg for total RNAs</li>
<li><strong>High library complexity</strong>:<strong> </strong>obtain a complete view of&nbsp;your small RNA transcriptome</li>
<li><strong>Optimal performance on clinical samples</strong>: validated&nbsp;with circulating RNAs from liquid biopsies</li>
<li><strong>Easy to use with minimal hands-on time</strong>: one day, one tube protocol</li>
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<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-b">C05030061 - D-Plex 24 DNBSEQ Barcodes for MGI - Set B</a></li>
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<p></p>
<p><span style="font-weight: 400;">D-Plex DNBSEQ</span><span><span>&nbsp;</span></span><span style="font-weight: 400;">Barcodes - Set B<span>&nbsp;</span><span>includes primers with&nbsp;</span><span>24</span><span><span>&nbsp;barcodes</span>&nbsp;to enable multiplexing</span><span>&nbsp;with</span>&nbsp;the<span>&nbsp;</span></span><a href="https://www.diagenode.com/en/p/d-plex-small-RNA-dnbseq-kit-x24">D-Plex Small RNA DNBSEQ<sup>TM</sup><span>&nbsp;</span>Kit</a><span style="font-weight: 400;">. They were designed and validated to fit the D-Plex technology for MGI sequencers.</span></p>
<p>Two sets are available separately:</p>
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<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-a">D-Plex DNBSEQ Barcodes - Set A</a></li>
<li>D-Plex DNBSEQ Barcodes - Set B</li>
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<p>Set A can be used for library multiplexing up to 24. Set A and Set B can be used simultaneously for library multiplexing up to 48.</p>
<p><span>Read more about the&nbsp;</span><a href="https://www.diagenode.com/en/categories/Library-preparation-for-RNA-seq">D-Plex technology</a><b>.</b></p>',
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<p>Diagenode&rsquo;s MicroChIP DiaPure columns have been optimized for the purification and elution of very low amounts of DNA. This rapid method has been validated for epigenetic applications like low input ChIP (e.g. using the True MicroChIP kit) and CUT&amp;Tag (e.g. using Diagenode&rsquo;s pA-Tn5), but is also compatible with many other applications.&nbsp; The DNA can be eluted at high concentrations in volumes down to 6 &mu;l and it is suitable for any downstream application (e.g. NGS).</p>
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			'info1' => '<h2 style="text-align: center;">MicroChIP DiaPure columns after ChIP</h2>
<p>Successful ChIP-seq results generated on 50,000 of K562 cells using True MicroChIP technology. ChIP has been performed accordingly to True MicroChIP protocol (Diagenode, Cat. No. C01010130), including DNA purification using the MicroChIP DiaPure columns. For the library preparation the MicroPlex Library Preparation Kit (Diagenode, Cat. No. C05010001) has been used. The below figure shows the peaks from ChIP-seq experiments using the following Diagenode antibodies: H3K4me1 (C15410194), H3K9/14ac (C15410200), H3K27ac (C15410196) and H3K36me3 (C15410192).</p>
<p><img src="https://www.diagenode.com/img/product/kits/figure-igv-microchip.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 1:</strong> Integrative genomics viewer (IGV) visualization of ChIP-seq experiments using 50,000 of K562 cells.</p>
<p></p>
<h2 style="text-align: center;"></h2>
<h2 style="text-align: center;">MicroChIP DiaPure columns after CUT&amp;Tag</h2>
<p>Successful CUT&amp;Tag results showing a low background with high region-specific enrichment has been generated using 50.000 of K562 cells, 1 &micro;g of H3K27me3 antibody (Diagenode, Cat. No. C15410069) and proteinA-Tn5 (1:250) (Diagenode, Cat. No. C01070001). 1 &micro;g of IgG (Diagenode, Cat. No. C15410206) was used as negative control. Samples were purified using the MicroChIP DiaPure columns or phenol-chloroform purification. The below figure presenst the comparison of two purification methods.</p>
<p><img src="https://www.diagenode.com/img/product/kits/figure-diapure-igv.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 2:</strong> Integrative genomics viewer (IGV) visualization of CUT&amp;Tag experiments: MicroChIP DiaPure columns vs phenol-chloroform purification using the H3K27me3 antibody.</p>',
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<p><strong>Figure 2:</strong> Integrative genomics viewer (IGV) visualization of CUT&amp;Tag experiments: MicroChIP DiaPure columns vs phenol-chloroform purification using the H3K27me3 antibody.</p>',
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