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<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/D-Plex-Small-RNA-DNBSEQ.pdf" target="_blank" title="D-Plex Small RNA DNBSEQ user manual"><strong>Download the manual</strong></a></center>
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<p>D-Plex Small RNA DNBSEQ™ Kit is a tool designed for the study of the small non-coding transcriptome. The kit is using the <a href="https://www.diagenode.com/en/pages/dplex" target="_blank">D-Plex technology</a> to generate double-stranded DNA libraries ready to be used for the DNA single-strand circularization step required for DNBSEQ sequencing on MGI sequencers.</p>
<p>The D-Plex technology utilizes the innovative capture and amplification by tailing and switching, a ligation-free method for RNA library preparation from ultra-low input amounts, down to 10 pg for small RNAs and 100 pg for total RNAs. This innovative solution enables diverse and novel transcripts detection, even from challenging clinical samples such as liquid biopsies.</p>
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<p>D-Plex Small RNA DNBSEQ™ Kit includes all buffers and enzymes necessary for the library preparation. Specific D-Plex DNBSEQ Barcodes were designed and validated to fit the D-Plex technology and are available separately:</p>
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<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-a">C05030060 - D-Plex DNBSEQ Barcodes for MGI - Set A</a></li>
<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-b">C05030061 - D-Plex DNBSEQ Barcodes for MGI - Set B</a></li>
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<p><b><strong>D-Plex is also available for Illumina sequencing, check<span> </span><a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24" target="_blank">here</a>!</strong></b></p>
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<h3>Diverse and novel transcript detection</h3>
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<p style="text-align: justify;">The D-Plex Small RNA DNBSEQ protocol generates complex RNA libraries deciphering the wide diversity of small non-coding RNA spectrum (including miRNAs, snoRNAs, snRNAs) in human plasma samples.</p>
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<h3>Ultra-low input performance</h3>
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<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p style="text-align: justify;">Sequencing data from circulating RNA samples of two input amounts (25 pg and 2.5 ng) were highly correlated (<i>R = 0.99</i>) when compared using Pearson correlation coefficient.</p>
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<h3>High mapping efficiency</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px; display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-mapping.png" alt="High mapping efficiency" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p style="text-align: justify;"><b></b>The D-Plex Small RNA DNBSEQ kit is compatible with clinical-relevant samples, such as human plasma, and ultra low range of circulating RNA input (down to 10 pg) and exhibits good read mapping of sequencing reads (up to 70% mapping rate).</p>
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<h3>High quality DNBSEQ sequencing solution</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-dnbseq.png" alt="High quality DNBSEQ sequencing solution" /></div>
<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p><b></b>The D-Plex Small RNA DNBSEQ kit combines our best-in-class RNA library preparation – D-Plex technology – with MGI’s high-quality, cost-effective, DNA nanoballs – DNBSEQ – sequencing solution, creating a unified platform to support high quality small RNA sequencing.</p>
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<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-b">C05030061 - D-Plex 24 DNBSEQ Barcodes for MGI - Set B</a></li>
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<p><span style="font-weight: 400;">D-Plex DNBSEQ</span><span><span> </span></span><span style="font-weight: 400;">Barcodes - Set B<span> </span><span>includes primers with </span><span>24</span><span><span> barcodes</span> to enable multiplexing</span><span> with</span> the<span> </span></span><a href="https://www.diagenode.com/en/p/d-plex-small-RNA-dnbseq-kit-x24">D-Plex Small RNA DNBSEQ<sup>TM</sup><span> </span>Kit</a><span style="font-weight: 400;">. They were designed and validated to fit the D-Plex technology for MGI sequencers.</span></p>
<p>Two sets are available separately:</p>
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<p>Set A can be used for library multiplexing up to 24. Set A and Set B can be used simultaneously for library multiplexing up to 48.</p>
<p><span>Read more about the </span><a href="https://www.diagenode.com/en/categories/Library-preparation-for-RNA-seq">D-Plex technology</a><b>.</b></p>',
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<p>Diagenode’s MicroChIP DiaPure columns have been optimized for the purification and elution of very low amounts of DNA. This rapid method has been validated for epigenetic applications like low input ChIP (e.g. using the True MicroChIP kit) and CUT&Tag (e.g. using Diagenode’s pA-Tn5), but is also compatible with many other applications. The DNA can be eluted at high concentrations in volumes down to 6 μl and it is suitable for any downstream application (e.g. NGS).</p>
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<p><img src="https://www.diagenode.com/img/product/kits/figure-igv-microchip.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 1:</strong> Integrative genomics viewer (IGV) visualization of ChIP-seq experiments using 50,000 of K562 cells.</p>
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<h2 style="text-align: center;"></h2>
<h2 style="text-align: center;">MicroChIP DiaPure columns after CUT&Tag</h2>
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<p><img src="https://www.diagenode.com/img/product/kits/figure-diapure-igv.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 2:</strong> Integrative genomics viewer (IGV) visualization of CUT&Tag experiments: MicroChIP DiaPure columns vs phenol-chloroform purification using the H3K27me3 antibody.</p>',
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<p><img src="https://www.diagenode.com/img/product/kits/figure-diapure-igv.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 2:</strong> Integrative genomics viewer (IGV) visualization of CUT&Tag experiments: MicroChIP DiaPure columns vs phenol-chloroform purification using the H3K27me3 antibody.</p>',
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View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
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<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/D-Plex-Small-RNA-DNBSEQ.pdf" target="_blank" title="D-Plex Small RNA DNBSEQ user manual"><strong>Download the manual</strong></a></center>
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<p>D-Plex Small RNA DNBSEQ™ Kit is a tool designed for the study of the small non-coding transcriptome. The kit is using the <a href="https://www.diagenode.com/en/pages/dplex" target="_blank">D-Plex technology</a> to generate double-stranded DNA libraries ready to be used for the DNA single-strand circularization step required for DNBSEQ sequencing on MGI sequencers.</p>
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<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-b">C05030061 - D-Plex DNBSEQ Barcodes for MGI - Set B</a></li>
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<p><b><strong>D-Plex is also available for Illumina sequencing, check<span> </span><a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24" target="_blank">here</a>!</strong></b></p>
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<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px; display: block; margin-left: auto; margin-right: auto;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-transcript.png" alt="small RNA library preparation for Illumina" /></div>
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<p style="text-align: justify;">The D-Plex Small RNA DNBSEQ protocol generates complex RNA libraries deciphering the wide diversity of small non-coding RNA spectrum (including miRNAs, snoRNAs, snRNAs) in human plasma samples.</p>
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<h3>Ultra-low input performance</h3>
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<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p style="text-align: justify;">Sequencing data from circulating RNA samples of two input amounts (25 pg and 2.5 ng) were highly correlated (<i>R = 0.99</i>) when compared using Pearson correlation coefficient.</p>
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<h3>High mapping efficiency</h3>
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<div class="large-8 small-12 medium-6 large-centered medium-centered small-centered columns"><center>
<p style="text-align: justify;"><b></b>The D-Plex Small RNA DNBSEQ kit is compatible with clinical-relevant samples, such as human plasma, and ultra low range of circulating RNA input (down to 10 pg) and exhibits good read mapping of sequencing reads (up to 70% mapping rate).</p>
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<h3>High quality DNBSEQ sequencing solution</h3>
<div class="large-10 small-12 medium-10 large-centered medium-centered small-centered columns"><img style="height: 400px;" src="https://www.diagenode.com/img/product/kits/dplex/dplex-dnbseq.png" alt="High quality DNBSEQ sequencing solution" /></div>
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<p><b></b>The D-Plex Small RNA DNBSEQ kit combines our best-in-class RNA library preparation – D-Plex technology – with MGI’s high-quality, cost-effective, DNA nanoballs – DNBSEQ – sequencing solution, creating a unified platform to support high quality small RNA sequencing.</p>
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<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-b">C05030061 - D-Plex 24 DNBSEQ Barcodes for MGI - Set B</a></li>
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<p><span style="font-weight: 400;">D-Plex DNBSEQ</span><span><span> </span></span><span style="font-weight: 400;">Barcodes - Set B<span> </span><span>includes primers with </span><span>24</span><span><span> barcodes</span> to enable multiplexing</span><span> with</span> the<span> </span></span><a href="https://www.diagenode.com/en/p/d-plex-small-RNA-dnbseq-kit-x24">D-Plex Small RNA DNBSEQ<sup>TM</sup><span> </span>Kit</a><span style="font-weight: 400;">. They were designed and validated to fit the D-Plex technology for MGI sequencers.</span></p>
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<p>Diagenode’s MicroChIP DiaPure columns have been optimized for the purification and elution of very low amounts of DNA. This rapid method has been validated for epigenetic applications like low input ChIP (e.g. using the True MicroChIP kit) and CUT&Tag (e.g. using Diagenode’s pA-Tn5), but is also compatible with many other applications. The DNA can be eluted at high concentrations in volumes down to 6 μl and it is suitable for any downstream application (e.g. NGS).</p>
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<p><strong>Figure 1:</strong> Integrative genomics viewer (IGV) visualization of ChIP-seq experiments using 50,000 of K562 cells.</p>
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<h2 style="text-align: center;">MicroChIP DiaPure columns after CUT&Tag</h2>
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<p><img src="https://www.diagenode.com/img/product/kits/figure-diapure-igv.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 2:</strong> Integrative genomics viewer (IGV) visualization of CUT&Tag experiments: MicroChIP DiaPure columns vs phenol-chloroform purification using the H3K27me3 antibody.</p>',
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<p>Diagenode’s MicroChIP DiaPure columns have been optimized for the purification and elution of very low amounts of DNA. This rapid method has been validated for epigenetic applications like low input ChIP (e.g. using the True MicroChIP kit) and CUT&Tag (e.g. using Diagenode’s pA-Tn5), but is also compatible with many other applications. The DNA can be eluted at high concentrations in volumes down to 6 μl and it is suitable for any downstream application (e.g. NGS).</p>
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<p><img src="https://www.diagenode.com/img/product/kits/figure-diapure-igv.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 2:</strong> Integrative genomics viewer (IGV) visualization of CUT&Tag experiments: MicroChIP DiaPure columns vs phenol-chloroform purification using the H3K27me3 antibody.</p>',
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View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
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<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/D-Plex-Small-RNA-DNBSEQ.pdf" target="_blank" title="D-Plex Small RNA DNBSEQ user manual"><strong>Download the manual</strong></a></center>
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<p>D-Plex Small RNA DNBSEQ™ Kit is a tool designed for the study of the small non-coding transcriptome. The kit is using the <a href="https://www.diagenode.com/en/pages/dplex" target="_blank">D-Plex technology</a> to generate double-stranded DNA libraries ready to be used for the DNA single-strand circularization step required for DNBSEQ sequencing on MGI sequencers.</p>
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<p><b><strong>D-Plex is also available for Illumina sequencing, check<span> </span><a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24" target="_blank">here</a>!</strong></b></p>
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<p style="text-align: justify;">The D-Plex Small RNA DNBSEQ protocol generates complex RNA libraries deciphering the wide diversity of small non-coding RNA spectrum (including miRNAs, snoRNAs, snRNAs) in human plasma samples.</p>
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<p style="text-align: justify;">Sequencing data from circulating RNA samples of two input amounts (25 pg and 2.5 ng) were highly correlated (<i>R = 0.99</i>) when compared using Pearson correlation coefficient.</p>
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<p style="text-align: justify;"><b></b>The D-Plex Small RNA DNBSEQ kit is compatible with clinical-relevant samples, such as human plasma, and ultra low range of circulating RNA input (down to 10 pg) and exhibits good read mapping of sequencing reads (up to 70% mapping rate).</p>
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<h3>High quality DNBSEQ sequencing solution</h3>
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<p><b></b>The D-Plex Small RNA DNBSEQ kit combines our best-in-class RNA library preparation – D-Plex technology – with MGI’s high-quality, cost-effective, DNA nanoballs – DNBSEQ – sequencing solution, creating a unified platform to support high quality small RNA sequencing.</p>
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<p><span style="font-weight: 400;">D-Plex DNBSEQ</span><span><span> </span></span><span style="font-weight: 400;">Barcodes - Set B<span> </span><span>includes primers with </span><span>24</span><span><span> barcodes</span> to enable multiplexing</span><span> with</span> the<span> </span></span><a href="https://www.diagenode.com/en/p/d-plex-small-RNA-dnbseq-kit-x24">D-Plex Small RNA DNBSEQ<sup>TM</sup><span> </span>Kit</a><span style="font-weight: 400;">. They were designed and validated to fit the D-Plex technology for MGI sequencers.</span></p>
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<p><strong>Figure 2:</strong> Integrative genomics viewer (IGV) visualization of CUT&Tag experiments: MicroChIP DiaPure columns vs phenol-chloroform purification using the H3K27me3 antibody.</p>',
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<p>Diagenode’s MicroChIP DiaPure columns have been optimized for the purification and elution of very low amounts of DNA. This rapid method has been validated for epigenetic applications like low input ChIP (e.g. using the True MicroChIP kit) and CUT&Tag (e.g. using Diagenode’s pA-Tn5), but is also compatible with many other applications. The DNA can be eluted at high concentrations in volumes down to 6 μl and it is suitable for any downstream application (e.g. NGS).</p>
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<p><img src="https://www.diagenode.com/img/product/kits/figure-igv-microchip.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><img src="https://www.diagenode.com/img/product/kits/figure-diapure-igv.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 2:</strong> Integrative genomics viewer (IGV) visualization of CUT&Tag experiments: MicroChIP DiaPure columns vs phenol-chloroform purification using the H3K27me3 antibody.</p>',
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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<center><a class="chip diahome button" href="https://www.diagenode.com/files/products/kits/D-Plex-Small-RNA-DNBSEQ.pdf" target="_blank" title="D-Plex Small RNA DNBSEQ user manual"><strong>Download the manual</strong></a></center>
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<p>D-Plex Small RNA DNBSEQ™ Kit is a tool designed for the study of the small non-coding transcriptome. The kit is using the <a href="https://www.diagenode.com/en/pages/dplex" target="_blank">D-Plex technology</a> to generate double-stranded DNA libraries ready to be used for the DNA single-strand circularization step required for DNBSEQ sequencing on MGI sequencers.</p>
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<li><a href="https://www.diagenode.com/en/p/d-plex-24-dnbseq-barcodes-set-b">C05030061 - D-Plex DNBSEQ Barcodes for MGI - Set B</a></li>
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<p><b><strong>D-Plex is also available for Illumina sequencing, check<span> </span><a href="https://www.diagenode.com/en/p/D-Plex-Small-RNA-seq-Library-Prep-x24" target="_blank">here</a>!</strong></b></p>
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<p style="text-align: justify;">The D-Plex Small RNA DNBSEQ protocol generates complex RNA libraries deciphering the wide diversity of small non-coding RNA spectrum (including miRNAs, snoRNAs, snRNAs) in human plasma samples.</p>
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<p style="text-align: justify;">Sequencing data from circulating RNA samples of two input amounts (25 pg and 2.5 ng) were highly correlated (<i>R = 0.99</i>) when compared using Pearson correlation coefficient.</p>
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<p style="text-align: justify;"><b></b>The D-Plex Small RNA DNBSEQ kit is compatible with clinical-relevant samples, such as human plasma, and ultra low range of circulating RNA input (down to 10 pg) and exhibits good read mapping of sequencing reads (up to 70% mapping rate).</p>
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<p><b></b>The D-Plex Small RNA DNBSEQ kit combines our best-in-class RNA library preparation – D-Plex technology – with MGI’s high-quality, cost-effective, DNA nanoballs – DNBSEQ – sequencing solution, creating a unified platform to support high quality small RNA sequencing.</p>
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<p><span style="font-weight: 400;">D-Plex DNBSEQ</span><span><span> </span></span><span style="font-weight: 400;">Barcodes - Set B<span> </span><span>includes primers with </span><span>24</span><span><span> barcodes</span> to enable multiplexing</span><span> with</span> the<span> </span></span><a href="https://www.diagenode.com/en/p/d-plex-small-RNA-dnbseq-kit-x24">D-Plex Small RNA DNBSEQ<sup>TM</sup><span> </span>Kit</a><span style="font-weight: 400;">. They were designed and validated to fit the D-Plex technology for MGI sequencers.</span></p>
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<p><strong>Figure 2:</strong> Integrative genomics viewer (IGV) visualization of CUT&Tag experiments: MicroChIP DiaPure columns vs phenol-chloroform purification using the H3K27me3 antibody.</p>',
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<p>将 <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> D-Plex DNBSEQ Barcodes for MGI Sequencing - Set B</strong> 添加至我的购物车。</p>
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<button class="alert small button expand" onclick="$(this).addToCart('D-Plex DNBSEQ Barcodes for MGI Sequencing - Set B',
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<p>Successful ChIP-seq results generated on 50,000 of K562 cells using True MicroChIP technology. ChIP has been performed accordingly to True MicroChIP protocol (Diagenode, Cat. No. C01010130), including DNA purification using the MicroChIP DiaPure columns. For the library preparation the MicroPlex Library Preparation Kit (Diagenode, Cat. No. C05010001) has been used. The below figure shows the peaks from ChIP-seq experiments using the following Diagenode antibodies: H3K4me1 (C15410194), H3K9/14ac (C15410200), H3K27ac (C15410196) and H3K36me3 (C15410192).</p>
<p><img src="https://www.diagenode.com/img/product/kits/figure-igv-microchip.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
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<p><img src="https://www.diagenode.com/img/product/kits/figure-diapure-igv.png" style="display: block; margin-left: auto; margin-right: auto;" /></p>
<p><strong>Figure 2:</strong> Integrative genomics viewer (IGV) visualization of CUT&Tag experiments: MicroChIP DiaPure columns vs phenol-chloroform purification using the H3K27me3 antibody.</p>',
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View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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