| Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor® Standard, Plus or Pico This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chro... | Download |
| DNA Elution Module MANUAL The DNA elution module allows the elution of chromatin and DNA from immunoprecipitated ma... | Download |
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Hyper-Editing of Cell-Cycle Regulatory and Tumor Suppressor RNA Promotes Malignant Progenitor Propagation. |
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The following protocol is optimized for 30-40 mg of tissue allowing up to 18 ChIP samples (1.5-2 mg of tissue per sample). 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Here we show that ADAR1-induced hyper-editing in normal human hematopoietic progenitors impairs miR-26a maturation, which represses CDKN1A expression indirectly via EZH2, thereby accelerating cell-cycle transit. However, in blast crisis chronic myeloid leukemia progenitors, loss of EZH2 expression and increased CDKN1A oppose cell-cycle transit. Moreover, A-to-I editing of both the MDM2 regulatory microRNA and its binding site within the 3' UTR region stabilizes MDM2 transcripts, thereby enhancing blast crisis progenitor propagation. These data reveal a dual mechanism governing malignant transformation of progenitors that is predicated on hyper-editing of cell-cycle-regulatory miRNAs and the 3' UTR binding site of tumor suppressor miRNAs.</p>', 'date' => '2019-01-14', 'pmid' => 'http://www.pubmed.gov/30612940', 'doi' => '10.1016/j.ccell.2018.11.017', 'modified' => '2019-05-08 12:25:16', 'created' => '2019-04-25 11:11:44', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '606', 'name' => 'Elution Module SDS GB en', 'language' => 'en', 'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-GB-en-1_0.pdf', 'countries' => 'GB', 'modified' => '2020-07-01 15:35:16', 'created' => '2020-07-01 15:35:16', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '608', 'name' => 'Elution Module SDS US en', 'language' => 'en', 'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-US-en-1_0.pdf', 'countries' => 'US', 'modified' => '2020-07-01 15:36:01', 'created' => '2020-07-01 15:36:01', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '603', 'name' => 'Elution Module SDS DE de', 'language' => 'de', 'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-DE-de-1_0.pdf', 'countries' => 'DE', 'modified' => '2020-07-01 15:34:04', 'created' => '2020-07-01 15:34:04', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '607', 'name' => 'Elution Module SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-JP-ja-1_1.pdf', 'countries' => 'JP', 'modified' => '2020-07-01 15:35:39', 'created' => '2020-07-01 15:35:39', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '602', 'name' => 'Elution Module SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-BE-nl-1_0.pdf', 'countries' => 'BE', 'modified' => '2020-07-01 15:33:26', 'created' => '2020-07-01 15:33:26', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '601', 'name' => 'Elution Module SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-BE-fr-1_0.pdf', 'countries' => 'BE', 'modified' => '2020-07-01 15:32:52', 'created' => '2020-07-01 15:32:52', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '605', 'name' => 'Elution Module SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-FR-fr-1_0.pdf', 'countries' => 'FR', 'modified' => '2020-07-01 15:34:55', 'created' => '2020-07-01 15:34:55', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '604', 'name' => 'Elution Module SDS ES es', 'language' => 'es', 'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-ES-es-1_0.pdf', 'countries' => 'ES', 'modified' => '2020-07-01 15:34:32', 'created' => '2020-07-01 15:34:32', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array() $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = '<br/><small><span style="color:#CCC">(mc-magme-002)</span></small>' $country_code = 'US' $img = 'banners/banner-cut_tag-chipmentation-500.jpg' $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $protocol = array( 'id' => '1', 'name' => 'Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor<sup>®</sup> Standard, Plus or Pico', 'description' => '<p>This protocol describes the chromatin preparation from fresh or frozen tissues. 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This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p> <p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p> </div> <div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div> <div class="small-12 medium-12 large-12 columns"><br /> <ol> <li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li> <li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li> <li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li> <li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li> <li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li> </ol> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"> <h3 class="text-center" style="color: #b21329;">Need guidance?</h3> <p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. 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ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of', 'meta_title' => 'ChIP Quantitative PCR (ChIP-qPCR) | Diagenode', 'modified' => '2018-01-09 16:46:56', 'created' => '2014-12-11 00:22:08', 'ProductsApplication' => array( 'id' => '2134', 'product_id' => '1855', 'application_id' => '10' ) ) $slugs = array( (int) 0 => 'chip-qpcr' ) $applications = array( 'id' => '10', 'position' => '10', 'parent_id' => '2', 'name' => 'ChIP-qPCR', 'description' => '<div class="row"> <div class="row">定量のPCRとクロマチン免疫沈降（ChIP）が相まり、既知のゲノム結合部位でのタンパク質-DNA相互作用を調べる事に利用できます。ゲノム結合部位が不明の場合は、プロモーターのような潜在的な制御領域に対してqPCRプライマーを設計することもできます。ChIP-qPCRは、リアルタイムPCRを実行するコストが最小であるため、異なる実験条件にわたって特定の遺伝子および潜在的な制御領域に焦点を当てた研究においてとても有利です。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、および遺伝子発現に対するヒストン修飾の効果を含む様々なライフサイエンス分野で使用されています。<br /> <div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div> <div class="small-12 medium-12 large-12 columns"><br /> <ol> <li class="large-12 columns"><strong>Chromatin preparation (クロマチン調製):<span> </span></strong>DNAへのヒストンまたは転写因子などのクロマチン結合タンパク質の固定（架橋）に続いて細胞溶解。</li> <li class="large-12 columns"><strong><strong><strong>Chromatin shearing (クロマチン断片化):<span> </span></strong></strong></strong>超音波処理による所望の断片サイズ（100〜500bp）までのクロマチンの断片化</li> <li class="large-12 columns"><strong>Chromatin IP (クロマチン免疫沈降):</strong><span> </span>目的のヒストンまたは転写因子に対する<strong><strong><a href="./chip-qpcr-antibodies">特定のChIP級抗体</a></strong></strong> <p>を用いたタンパク質-DNA複合体の捕捉</p> </li> <li class="large-12 columns"><strong>DNA purification (DNA精製):<span> </span></strong>クロマチン逆架橋および溶出後の精製</li> <li class="large-12 columns"><strong>qPCR and analysis (qPCRおよび分析):</strong><span> </span>以前に設計されたプライマーを使用して、特定の遺伝子座位で免疫沈降した物質を増幅する</li> </ol> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"> <h3 class="text-center" style="color: #b21329;">初めての方へ</h3> <p>当社の完全なChIPキットを選択頂くか、個別で抗体、バッファー、ビーズ、クロマチン断片および精製試薬から必要なものを選択頂けます。ChIP Kit Customizerを使用すると、検証済みのChIPキットから必要なアイテムを自由に選択できます。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div> <div class="small-6 medium-6 large-6 columns"><a href="../pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div> </div> </div> </div> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"></div> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr', 'meta_keywords' => 'Diagenode ChIP qPCRキットは、染色体免疫沈降後の豊富なDNAを定量化。', 'meta_description' => 'Diagenode's ChIP qPCR kits can be used to quantify enriched DNA after chromatin immunoprecipitation. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of', 'meta_title' => 'クロマチン免疫沈降（ChIP）および定量PCR | Diagenode', 'modified' => '2018-01-09 16:46:56', 'created' => '2014-12-11 00:22:08', 'locale' => 'jpn' ) $description = '<div class="row"> <div class="row">定量のPCRとクロマチン免疫沈降（ChIP）が相まり、既知のゲノム結合部位でのタンパク質-DNA相互作用を調べる事に利用できます。ゲノム結合部位が不明の場合は、プロモーターのような潜在的な制御領域に対してqPCRプライマーを設計することもできます。ChIP-qPCRは、リアルタイムPCRを実行するコストが最小であるため、異なる実験条件にわたって特定の遺伝子および潜在的な制御領域に焦点を当てた研究においてとても有利です。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、および遺伝子発現に対するヒストン修飾の効果を含む様々なライフサイエンス分野で使用されています。<br /> <div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div> <div class="small-12 medium-12 large-12 columns"><br /> <ol> <li class="large-12 columns"><strong>Chromatin preparation (クロマチン調製):<span> </span></strong>DNAへのヒストンまたは転写因子などのクロマチン結合タンパク質の固定（架橋）に続いて細胞溶解。</li> <li class="large-12 columns"><strong><strong><strong>Chromatin shearing (クロマチン断片化):<span> </span></strong></strong></strong>超音波処理による所望の断片サイズ（100〜500bp）までのクロマチンの断片化</li> <li class="large-12 columns"><strong>Chromatin IP (クロマチン免疫沈降):</strong><span> </span>目的のヒストンまたは転写因子に対する<strong><strong><a href="./chip-qpcr-antibodies">特定のChIP級抗体</a></strong></strong> <p>を用いたタンパク質-DNA複合体の捕捉</p> </li> <li class="large-12 columns"><strong>DNA purification (DNA精製):<span> </span></strong>クロマチン逆架橋および溶出後の精製</li> <li class="large-12 columns"><strong>qPCR and analysis (qPCRおよび分析):</strong><span> </span>以前に設計されたプライマーを使用して、特定の遺伝子座位で免疫沈降した物質を増幅する</li> </ol> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"> <h3 class="text-center" style="color: #b21329;">初めての方へ</h3> <p>当社の完全なChIPキットを選択頂くか、個別で抗体、バッファー、ビーズ、クロマチン断片および精製試薬から必要なものを選択頂けます。ChIP Kit Customizerを使用すると、検証済みのChIPキットから必要なアイテムを自由に選択できます。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div> <div class="small-6 medium-6 large-6 columns"><a href="../pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div> </div> </div> </div> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"></div> </div> </div>' $name = 'ChIP-qPCR' $document = array( 'id' => '68', 'name' => 'DNA Elution Module', 'description' => '<div class="page" title="Page 4"> <div class="section"> <div class="layoutArea"> <div class="column"> <p><span>The DNA elution module allows the elution of chromatin and DNA from immunoprecipitated material after ChIP and MeDIP respectively. Afterwards, you can proceed to the DNA purification using columns or carrying out a phenol/ chloroform/isomayl alcohol extraction. </span></p> </div> </div> </div> </div>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/kits/Elution_Module_manual.pdf', 'slug' => 'elution-module-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-09-01 11:55:17', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( 'id' => '280', 'product_id' => '1855', 'document_id' => '68' ) ) $sds = array( 'id' => '604', 'name' => 'Elution Module SDS ES es', 'language' => 'es', 'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-ES-es-1_0.pdf', 'countries' => 'ES', 'modified' => '2020-07-01 15:34:32', 'created' => '2020-07-01 15:34:32', 'ProductsSafetySheet' => array( 'id' => '1139', 'product_id' => '1855', 'safety_sheet_id' => '604' ) ) $publication = array( 'id' => '3630', 'name' => 'Hyper-Editing of Cell-Cycle Regulatory and Tumor Suppressor RNA Promotes Malignant Progenitor Propagation.', 'authors' => 'Jiang Q, Isquith J, Zipeto MA, Diep RH, Pham J, Delos Santos N, Reynoso E, Chau J, Leu H, Lazzari E, Melese E, Ma W, Fang R, Minden M, Morris S, Ren B, Pineda G, Holm F, Jamieson C', 'description' => '<p>Adenosine deaminase associated with RNA1 (ADAR1) deregulation contributes to therapeutic resistance in many malignancies. Here we show that ADAR1-induced hyper-editing in normal human hematopoietic progenitors impairs miR-26a maturation, which represses CDKN1A expression indirectly via EZH2, thereby accelerating cell-cycle transit. However, in blast crisis chronic myeloid leukemia progenitors, loss of EZH2 expression and increased CDKN1A oppose cell-cycle transit. Moreover, A-to-I editing of both the MDM2 regulatory microRNA and its binding site within the 3' UTR region stabilizes MDM2 transcripts, thereby enhancing blast crisis progenitor propagation. These data reveal a dual mechanism governing malignant transformation of progenitors that is predicated on hyper-editing of cell-cycle-regulatory miRNAs and the 3' UTR binding site of tumor suppressor miRNAs.</p>', 'date' => '2019-01-14', 'pmid' => 'http://www.pubmed.gov/30612940', 'doi' => '10.1016/j.ccell.2018.11.017', 'modified' => '2019-05-08 12:25:16', 'created' => '2019-04-25 11:11:44', 'ProductsPublication' => array( 'id' => '3574', 'product_id' => '1855', 'publication_id' => '3630' ) ) $externalLink = ' <a href="http://www.pubmed.gov/30612940" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
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ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p> <p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p> </div> <div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div> <div class="small-12 medium-12 large-12 columns"><br /> <ol> <li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li> <li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li> <li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li> <li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li> <li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li> </ol> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"> <h3 class="text-center" style="color: #b21329;">Need guidance?</h3> <p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. 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ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of', 'meta_title' => 'ChIP Quantitative PCR (ChIP-qPCR) | Diagenode', 'modified' => '2018-01-09 16:46:56', 'created' => '2014-12-11 00:22:08', 'ProductsApplication' => array( 'id' => '2134', 'product_id' => '1855', 'application_id' => '10' ) ) $slugs = array( (int) 0 => 'chip-qpcr' ) $applications = array( 'id' => '10', 'position' => '10', 'parent_id' => '2', 'name' => 'ChIP-qPCR', 'description' => '<div class="row"> <div class="row">定量のPCRとクロマチン免疫沈降（ChIP）が相まり、既知のゲノム結合部位でのタンパク質-DNA相互作用を調べる事に利用できます。ゲノム結合部位が不明の場合は、プロモーターのような潜在的な制御領域に対してqPCRプライマーを設計することもできます。ChIP-qPCRは、リアルタイムPCRを実行するコストが最小であるため、異なる実験条件にわたって特定の遺伝子および潜在的な制御領域に焦点を当てた研究においてとても有利です。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、および遺伝子発現に対するヒストン修飾の効果を含む様々なライフサイエンス分野で使用されています。<br /> <div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div> <div class="small-12 medium-12 large-12 columns"><br /> <ol> <li class="large-12 columns"><strong>Chromatin preparation (クロマチン調製):<span> </span></strong>DNAへのヒストンまたは転写因子などのクロマチン結合タンパク質の固定（架橋）に続いて細胞溶解。</li> <li class="large-12 columns"><strong><strong><strong>Chromatin shearing (クロマチン断片化):<span> </span></strong></strong></strong>超音波処理による所望の断片サイズ（100〜500bp）までのクロマチンの断片化</li> <li class="large-12 columns"><strong>Chromatin IP (クロマチン免疫沈降):</strong><span> </span>目的のヒストンまたは転写因子に対する<strong><strong><a href="./chip-qpcr-antibodies">特定のChIP級抗体</a></strong></strong> <p>を用いたタンパク質-DNA複合体の捕捉</p> </li> <li class="large-12 columns"><strong>DNA purification (DNA精製):<span> </span></strong>クロマチン逆架橋および溶出後の精製</li> <li class="large-12 columns"><strong>qPCR and analysis (qPCRおよび分析):</strong><span> </span>以前に設計されたプライマーを使用して、特定の遺伝子座位で免疫沈降した物質を増幅する</li> </ol> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"> <h3 class="text-center" style="color: #b21329;">初めての方へ</h3> <p>当社の完全なChIPキットを選択頂くか、個別で抗体、バッファー、ビーズ、クロマチン断片および精製試薬から必要なものを選択頂けます。ChIP Kit Customizerを使用すると、検証済みのChIPキットから必要なアイテムを自由に選択できます。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div> <div class="small-6 medium-6 large-6 columns"><a href="../pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div> </div> </div> </div> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"></div> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr', 'meta_keywords' => 'Diagenode ChIP qPCRキットは、染色体免疫沈降後の豊富なDNAを定量化。', 'meta_description' => 'Diagenode's ChIP qPCR kits can be used to quantify enriched DNA after chromatin immunoprecipitation. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of', 'meta_title' => 'クロマチン免疫沈降（ChIP）および定量PCR | Diagenode', 'modified' => '2018-01-09 16:46:56', 'created' => '2014-12-11 00:22:08', 'locale' => 'jpn' ) $description = '<div class="row"> <div class="row">定量のPCRとクロマチン免疫沈降（ChIP）が相まり、既知のゲノム結合部位でのタンパク質-DNA相互作用を調べる事に利用できます。ゲノム結合部位が不明の場合は、プロモーターのような潜在的な制御領域に対してqPCRプライマーを設計することもできます。ChIP-qPCRは、リアルタイムPCRを実行するコストが最小であるため、異なる実験条件にわたって特定の遺伝子および潜在的な制御領域に焦点を当てた研究においてとても有利です。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、および遺伝子発現に対するヒストン修飾の効果を含む様々なライフサイエンス分野で使用されています。<br /> <div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div> <div class="small-12 medium-12 large-12 columns"><br /> <ol> <li class="large-12 columns"><strong>Chromatin preparation (クロマチン調製):<span> </span></strong>DNAへのヒストンまたは転写因子などのクロマチン結合タンパク質の固定（架橋）に続いて細胞溶解。</li> <li class="large-12 columns"><strong><strong><strong>Chromatin shearing (クロマチン断片化):<span> </span></strong></strong></strong>超音波処理による所望の断片サイズ（100〜500bp）までのクロマチンの断片化</li> <li class="large-12 columns"><strong>Chromatin IP (クロマチン免疫沈降):</strong><span> </span>目的のヒストンまたは転写因子に対する<strong><strong><a href="./chip-qpcr-antibodies">特定のChIP級抗体</a></strong></strong> <p>を用いたタンパク質-DNA複合体の捕捉</p> </li> <li class="large-12 columns"><strong>DNA purification (DNA精製):<span> </span></strong>クロマチン逆架橋および溶出後の精製</li> <li class="large-12 columns"><strong>qPCR and analysis (qPCRおよび分析):</strong><span> </span>以前に設計されたプライマーを使用して、特定の遺伝子座位で免疫沈降した物質を増幅する</li> </ol> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"> <h3 class="text-center" style="color: #b21329;">初めての方へ</h3> <p>当社の完全なChIPキットを選択頂くか、個別で抗体、バッファー、ビーズ、クロマチン断片および精製試薬から必要なものを選択頂けます。ChIP Kit Customizerを使用すると、検証済みのChIPキットから必要なアイテムを自由に選択できます。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div> <div class="small-6 medium-6 large-6 columns"><a href="../pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div> </div> </div> </div> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"></div> </div> </div>' $name = 'ChIP-qPCR' $document = array( 'id' => '68', 'name' => 'DNA Elution Module', 'description' => '<div class="page" title="Page 4"> <div class="section"> <div class="layoutArea"> <div class="column"> <p><span>The DNA elution module allows the elution of chromatin and DNA from immunoprecipitated material after ChIP and MeDIP respectively. 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Here we show that ADAR1-induced hyper-editing in normal human hematopoietic progenitors impairs miR-26a maturation, which represses CDKN1A expression indirectly via EZH2, thereby accelerating cell-cycle transit. However, in blast crisis chronic myeloid leukemia progenitors, loss of EZH2 expression and increased CDKN1A oppose cell-cycle transit. Moreover, A-to-I editing of both the MDM2 regulatory microRNA and its binding site within the 3' UTR region stabilizes MDM2 transcripts, thereby enhancing blast crisis progenitor propagation. These data reveal a dual mechanism governing malignant transformation of progenitors that is predicated on hyper-editing of cell-cycle-regulatory miRNAs and the 3' UTR binding site of tumor suppressor miRNAs.</p>', 'date' => '2019-01-14', 'pmid' => 'http://www.pubmed.gov/30612940', 'doi' => '10.1016/j.ccell.2018.11.017', 'modified' => '2019-05-08 12:25:16', 'created' => '2019-04-25 11:11:44', 'ProductsPublication' => array( 'id' => '3574', 'product_id' => '1855', 'publication_id' => '3630' ) ) $externalLink = ' <a href="http://www.pubmed.gov/30612940" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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The following protocol is optimized for 30-40 mg of tissue allowing up to 18 ChIP samples (1.5-2 mg of tissue per sample). 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Here we show that ADAR1-induced hyper-editing in normal human hematopoietic progenitors impairs miR-26a maturation, which represses CDKN1A expression indirectly via EZH2, thereby accelerating cell-cycle transit. However, in blast crisis chronic myeloid leukemia progenitors, loss of EZH2 expression and increased CDKN1A oppose cell-cycle transit. Moreover, A-to-I editing of both the MDM2 regulatory microRNA and its binding site within the 3' UTR region stabilizes MDM2 transcripts, thereby enhancing blast crisis progenitor propagation. 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This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p> <p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p> </div> <div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div> <div class="small-12 medium-12 large-12 columns"><br /> <ol> <li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li> <li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li> <li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li> <li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li> <li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li> </ol> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"> <h3 class="text-center" style="color: #b21329;">Need guidance?</h3> <p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. 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ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of', 'meta_title' => 'ChIP Quantitative PCR (ChIP-qPCR) | Diagenode', 'modified' => '2018-01-09 16:46:56', 'created' => '2014-12-11 00:22:08', 'ProductsApplication' => array( 'id' => '2134', 'product_id' => '1855', 'application_id' => '10' ) ) $slugs = array( (int) 0 => 'chip-qpcr' ) $applications = array( 'id' => '10', 'position' => '10', 'parent_id' => '2', 'name' => 'ChIP-qPCR', 'description' => '<div class="row"> <div class="row">定量のPCRとクロマチン免疫沈降（ChIP）が相まり、既知のゲノム結合部位でのタンパク質-DNA相互作用を調べる事に利用できます。ゲノム結合部位が不明の場合は、プロモーターのような潜在的な制御領域に対してqPCRプライマーを設計することもできます。ChIP-qPCRは、リアルタイムPCRを実行するコストが最小であるため、異なる実験条件にわたって特定の遺伝子および潜在的な制御領域に焦点を当てた研究においてとても有利です。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、および遺伝子発現に対するヒストン修飾の効果を含む様々なライフサイエンス分野で使用されています。<br /> <div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div> <div class="small-12 medium-12 large-12 columns"><br /> <ol> <li class="large-12 columns"><strong>Chromatin preparation (クロマチン調製):<span> </span></strong>DNAへのヒストンまたは転写因子などのクロマチン結合タンパク質の固定（架橋）に続いて細胞溶解。</li> <li class="large-12 columns"><strong><strong><strong>Chromatin shearing (クロマチン断片化):<span> </span></strong></strong></strong>超音波処理による所望の断片サイズ（100〜500bp）までのクロマチンの断片化</li> <li class="large-12 columns"><strong>Chromatin IP (クロマチン免疫沈降):</strong><span> </span>目的のヒストンまたは転写因子に対する<strong><strong><a href="./chip-qpcr-antibodies">特定のChIP級抗体</a></strong></strong> <p>を用いたタンパク質-DNA複合体の捕捉</p> </li> <li class="large-12 columns"><strong>DNA purification (DNA精製):<span> </span></strong>クロマチン逆架橋および溶出後の精製</li> <li class="large-12 columns"><strong>qPCR and analysis (qPCRおよび分析):</strong><span> </span>以前に設計されたプライマーを使用して、特定の遺伝子座位で免疫沈降した物質を増幅する</li> </ol> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"> <h3 class="text-center" style="color: #b21329;">初めての方へ</h3> <p>当社の完全なChIPキットを選択頂くか、個別で抗体、バッファー、ビーズ、クロマチン断片および精製試薬から必要なものを選択頂けます。ChIP Kit Customizerを使用すると、検証済みのChIPキットから必要なアイテムを自由に選択できます。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div> <div class="small-6 medium-6 large-6 columns"><a href="../pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div> </div> </div> </div> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"></div> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr', 'meta_keywords' => 'Diagenode ChIP qPCRキットは、染色体免疫沈降後の豊富なDNAを定量化。', 'meta_description' => 'Diagenode's ChIP qPCR kits can be used to quantify enriched DNA after chromatin immunoprecipitation. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of', 'meta_title' => 'クロマチン免疫沈降（ChIP）および定量PCR | Diagenode', 'modified' => '2018-01-09 16:46:56', 'created' => '2014-12-11 00:22:08', 'locale' => 'jpn' ) $description = '<div class="row"> <div class="row">定量のPCRとクロマチン免疫沈降（ChIP）が相まり、既知のゲノム結合部位でのタンパク質-DNA相互作用を調べる事に利用できます。ゲノム結合部位が不明の場合は、プロモーターのような潜在的な制御領域に対してqPCRプライマーを設計することもできます。ChIP-qPCRは、リアルタイムPCRを実行するコストが最小であるため、異なる実験条件にわたって特定の遺伝子および潜在的な制御領域に焦点を当てた研究においてとても有利です。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、および遺伝子発現に対するヒストン修飾の効果を含む様々なライフサイエンス分野で使用されています。<br /> <div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div> <div class="small-12 medium-12 large-12 columns"><br /> <ol> <li class="large-12 columns"><strong>Chromatin preparation (クロマチン調製):<span> </span></strong>DNAへのヒストンまたは転写因子などのクロマチン結合タンパク質の固定（架橋）に続いて細胞溶解。</li> <li class="large-12 columns"><strong><strong><strong>Chromatin shearing (クロマチン断片化):<span> </span></strong></strong></strong>超音波処理による所望の断片サイズ（100〜500bp）までのクロマチンの断片化</li> <li class="large-12 columns"><strong>Chromatin IP (クロマチン免疫沈降):</strong><span> </span>目的のヒストンまたは転写因子に対する<strong><strong><a href="./chip-qpcr-antibodies">特定のChIP級抗体</a></strong></strong> <p>を用いたタンパク質-DNA複合体の捕捉</p> </li> <li class="large-12 columns"><strong>DNA purification (DNA精製):<span> </span></strong>クロマチン逆架橋および溶出後の精製</li> <li class="large-12 columns"><strong>qPCR and analysis (qPCRおよび分析):</strong><span> </span>以前に設計されたプライマーを使用して、特定の遺伝子座位で免疫沈降した物質を増幅する</li> </ol> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"> <h3 class="text-center" style="color: #b21329;">初めての方へ</h3> <p>当社の完全なChIPキットを選択頂くか、個別で抗体、バッファー、ビーズ、クロマチン断片および精製試薬から必要なものを選択頂けます。ChIP Kit Customizerを使用すると、検証済みのChIPキットから必要なアイテムを自由に選択できます。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div> <div class="small-6 medium-6 large-6 columns"><a href="../pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div> </div> </div> </div> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"></div> </div> </div>' $name = 'ChIP-qPCR' $document = array( 'id' => '68', 'name' => 'DNA Elution Module', 'description' => '<div class="page" title="Page 4"> <div class="section"> <div class="layoutArea"> <div class="column"> <p><span>The DNA elution module allows the elution of chromatin and DNA from immunoprecipitated material after ChIP and MeDIP respectively. Afterwards, you can proceed to the DNA purification using columns or carrying out a phenol/ chloroform/isomayl alcohol extraction. </span></p> </div> </div> </div> </div>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/kits/Elution_Module_manual.pdf', 'slug' => 'elution-module-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-09-01 11:55:17', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( 'id' => '280', 'product_id' => '1855', 'document_id' => '68' ) ) $sds = array( 'id' => '604', 'name' => 'Elution Module SDS ES es', 'language' => 'es', 'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-ES-es-1_0.pdf', 'countries' => 'ES', 'modified' => '2020-07-01 15:34:32', 'created' => '2020-07-01 15:34:32', 'ProductsSafetySheet' => array( 'id' => '1139', 'product_id' => '1855', 'safety_sheet_id' => '604' ) ) $publication = array( 'id' => '3630', 'name' => 'Hyper-Editing of Cell-Cycle Regulatory and Tumor Suppressor RNA Promotes Malignant Progenitor Propagation.', 'authors' => 'Jiang Q, Isquith J, Zipeto MA, Diep RH, Pham J, Delos Santos N, Reynoso E, Chau J, Leu H, Lazzari E, Melese E, Ma W, Fang R, Minden M, Morris S, Ren B, Pineda G, Holm F, Jamieson C', 'description' => '<p>Adenosine deaminase associated with RNA1 (ADAR1) deregulation contributes to therapeutic resistance in many malignancies. Here we show that ADAR1-induced hyper-editing in normal human hematopoietic progenitors impairs miR-26a maturation, which represses CDKN1A expression indirectly via EZH2, thereby accelerating cell-cycle transit. However, in blast crisis chronic myeloid leukemia progenitors, loss of EZH2 expression and increased CDKN1A oppose cell-cycle transit. Moreover, A-to-I editing of both the MDM2 regulatory microRNA and its binding site within the 3' UTR region stabilizes MDM2 transcripts, thereby enhancing blast crisis progenitor propagation. These data reveal a dual mechanism governing malignant transformation of progenitors that is predicated on hyper-editing of cell-cycle-regulatory miRNAs and the 3' UTR binding site of tumor suppressor miRNAs.</p>', 'date' => '2019-01-14', 'pmid' => 'http://www.pubmed.gov/30612940', 'doi' => '10.1016/j.ccell.2018.11.017', 'modified' => '2019-05-08 12:25:16', 'created' => '2019-04-25 11:11:44', 'ProductsPublication' => array( 'id' => '3574', 'product_id' => '1855', 'publication_id' => '3630' ) ) $externalLink = ' <a href="http://www.pubmed.gov/30612940" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p> <p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p> </div> <div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div> <div class="small-12 medium-12 large-12 columns"><br /> <ol> <li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li> <li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li> <li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li> <li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li> <li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li> </ol> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"> <h3 class="text-center" style="color: #b21329;">Need guidance?</h3> <p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div> <div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div> </div> </div> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr', 'meta_keywords' => 'Chromatin immunoprecipitation,ChIP Quantitative PCR,polymerase chain reaction (PCR)', 'meta_description' => 'Diagenode's ChIP qPCR kits can be used to quantify enriched DNA after chromatin immunoprecipitation. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of', 'meta_title' => 'ChIP Quantitative PCR (ChIP-qPCR) | Diagenode', 'modified' => '2018-01-09 16:46:56', 'created' => '2014-12-11 00:22:08', 'ProductsApplication' => array( 'id' => '2134', 'product_id' => '1855', 'application_id' => '10' ) ) $slugs = array( (int) 0 => 'chip-qpcr' ) $applications = array( 'id' => '10', 'position' => '10', 'parent_id' => '2', 'name' => 'ChIP-qPCR', 'description' => '<div class="row"> <div class="row">定量のPCRとクロマチン免疫沈降（ChIP）が相まり、既知のゲノム結合部位でのタンパク質-DNA相互作用を調べる事に利用できます。ゲノム結合部位が不明の場合は、プロモーターのような潜在的な制御領域に対してqPCRプライマーを設計することもできます。ChIP-qPCRは、リアルタイムPCRを実行するコストが最小であるため、異なる実験条件にわたって特定の遺伝子および潜在的な制御領域に焦点を当てた研究においてとても有利です。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、および遺伝子発現に対するヒストン修飾の効果を含む様々なライフサイエンス分野で使用されています。<br /> <div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div> <div class="small-12 medium-12 large-12 columns"><br /> <ol> <li class="large-12 columns"><strong>Chromatin preparation (クロマチン調製):<span> </span></strong>DNAへのヒストンまたは転写因子などのクロマチン結合タンパク質の固定（架橋）に続いて細胞溶解。</li> <li class="large-12 columns"><strong><strong><strong>Chromatin shearing (クロマチン断片化):<span> </span></strong></strong></strong>超音波処理による所望の断片サイズ（100〜500bp）までのクロマチンの断片化</li> <li class="large-12 columns"><strong>Chromatin IP (クロマチン免疫沈降):</strong><span> </span>目的のヒストンまたは転写因子に対する<strong><strong><a href="./chip-qpcr-antibodies">特定のChIP級抗体</a></strong></strong> <p>を用いたタンパク質-DNA複合体の捕捉</p> </li> <li class="large-12 columns"><strong>DNA purification (DNA精製):<span> </span></strong>クロマチン逆架橋および溶出後の精製</li> <li class="large-12 columns"><strong>qPCR and analysis (qPCRおよび分析):</strong><span> </span>以前に設計されたプライマーを使用して、特定の遺伝子座位で免疫沈降した物質を増幅する</li> </ol> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"> <h3 class="text-center" style="color: #b21329;">初めての方へ</h3> <p>当社の完全なChIPキットを選択頂くか、個別で抗体、バッファー、ビーズ、クロマチン断片および精製試薬から必要なものを選択頂けます。ChIP Kit Customizerを使用すると、検証済みのChIPキットから必要なアイテムを自由に選択できます。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div> <div class="small-6 medium-6 large-6 columns"><a href="../pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div> </div> </div> </div> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"></div> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr', 'meta_keywords' => 'Diagenode ChIP qPCRキットは、染色体免疫沈降後の豊富なDNAを定量化。', 'meta_description' => 'Diagenode's ChIP qPCR kits can be used to quantify enriched DNA after chromatin immunoprecipitation. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of', 'meta_title' => 'クロマチン免疫沈降（ChIP）および定量PCR | Diagenode', 'modified' => '2018-01-09 16:46:56', 'created' => '2014-12-11 00:22:08', 'locale' => 'jpn' ) $description = '<div class="row"> <div class="row">定量のPCRとクロマチン免疫沈降（ChIP）が相まり、既知のゲノム結合部位でのタンパク質-DNA相互作用を調べる事に利用できます。ゲノム結合部位が不明の場合は、プロモーターのような潜在的な制御領域に対してqPCRプライマーを設計することもできます。ChIP-qPCRは、リアルタイムPCRを実行するコストが最小であるため、異なる実験条件にわたって特定の遺伝子および潜在的な制御領域に焦点を当てた研究においてとても有利です。また、この技術は現在、細胞分化、腫瘍抑制遺伝子のサイレンシング、および遺伝子発現に対するヒストン修飾の効果を含む様々なライフサイエンス分野で使用されています。<br /> <div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div> <div class="small-12 medium-12 large-12 columns"><br /> <ol> <li class="large-12 columns"><strong>Chromatin preparation (クロマチン調製):<span> </span></strong>DNAへのヒストンまたは転写因子などのクロマチン結合タンパク質の固定（架橋）に続いて細胞溶解。</li> <li class="large-12 columns"><strong><strong><strong>Chromatin shearing (クロマチン断片化):<span> </span></strong></strong></strong>超音波処理による所望の断片サイズ（100〜500bp）までのクロマチンの断片化</li> <li class="large-12 columns"><strong>Chromatin IP (クロマチン免疫沈降):</strong><span> </span>目的のヒストンまたは転写因子に対する<strong><strong><a href="./chip-qpcr-antibodies">特定のChIP級抗体</a></strong></strong> <p>を用いたタンパク質-DNA複合体の捕捉</p> </li> <li class="large-12 columns"><strong>DNA purification (DNA精製):<span> </span></strong>クロマチン逆架橋および溶出後の精製</li> <li class="large-12 columns"><strong>qPCR and analysis (qPCRおよび分析):</strong><span> </span>以前に設計されたプライマーを使用して、特定の遺伝子座位で免疫沈降した物質を増幅する</li> </ol> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"> <h3 class="text-center" style="color: #b21329;">初めての方へ</h3> <p>当社の完全なChIPキットを選択頂くか、個別で抗体、バッファー、ビーズ、クロマチン断片および精製試薬から必要なものを選択頂けます。ChIP Kit Customizerを使用すると、検証済みのChIPキットから必要なアイテムを自由に選択できます。</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div> <div class="small-6 medium-6 large-6 columns"><a href="../pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div> </div> </div> </div> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"></div> </div> </div>' $name = 'ChIP-qPCR' $document = array( 'id' => '68', 'name' => 'DNA Elution Module', 'description' => '<div class="page" title="Page 4"> <div class="section"> <div class="layoutArea"> <div class="column"> <p><span>The DNA elution module allows the elution of chromatin and DNA from immunoprecipitated material after ChIP and MeDIP respectively. Afterwards, you can proceed to the DNA purification using columns or carrying out a phenol/ chloroform/isomayl alcohol extraction. </span></p> </div> </div> </div> </div>', 'image_id' => null, 'type' => 'Manual', 'url' => 'files/products/kits/Elution_Module_manual.pdf', 'slug' => 'elution-module-manual', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-09-01 11:55:17', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( 'id' => '280', 'product_id' => '1855', 'document_id' => '68' ) ) $sds = array( 'id' => '604', 'name' => 'Elution Module SDS ES es', 'language' => 'es', 'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-ES-es-1_0.pdf', 'countries' => 'ES', 'modified' => '2020-07-01 15:34:32', 'created' => '2020-07-01 15:34:32', 'ProductsSafetySheet' => array( 'id' => '1139', 'product_id' => '1855', 'safety_sheet_id' => '604' ) ) $publication = array( 'id' => '3630', 'name' => 'Hyper-Editing of Cell-Cycle Regulatory and Tumor Suppressor RNA Promotes Malignant Progenitor Propagation.', 'authors' => 'Jiang Q, Isquith J, Zipeto MA, Diep RH, Pham J, Delos Santos N, Reynoso E, Chau J, Leu H, Lazzari E, Melese E, Ma W, Fang R, Minden M, Morris S, Ren B, Pineda G, Holm F, Jamieson C', 'description' => '<p>Adenosine deaminase associated with RNA1 (ADAR1) deregulation contributes to therapeutic resistance in many malignancies. Here we show that ADAR1-induced hyper-editing in normal human hematopoietic progenitors impairs miR-26a maturation, which represses CDKN1A expression indirectly via EZH2, thereby accelerating cell-cycle transit. However, in blast crisis chronic myeloid leukemia progenitors, loss of EZH2 expression and increased CDKN1A oppose cell-cycle transit. Moreover, A-to-I editing of both the MDM2 regulatory microRNA and its binding site within the 3' UTR region stabilizes MDM2 transcripts, thereby enhancing blast crisis progenitor propagation. These data reveal a dual mechanism governing malignant transformation of progenitors that is predicated on hyper-editing of cell-cycle-regulatory miRNAs and the 3' UTR binding site of tumor suppressor miRNAs.</p>', 'date' => '2019-01-14', 'pmid' => 'http://www.pubmed.gov/30612940', 'doi' => '10.1016/j.ccell.2018.11.017', 'modified' => '2019-05-08 12:25:16', 'created' => '2019-04-25 11:11:44', 'ProductsPublication' => array( 'id' => '3574', 'product_id' => '1855', 'publication_id' => '3630' ) ) $externalLink = ' <a href="http://www.pubmed.gov/30612940" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? 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