Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'cn',
'meta_keywords' => '',
'meta_description' => 'Elution Module',
'meta_title' => 'Elution Module',
'product' => array(
'Product' => array(
'id' => '1855',
'antibody_id' => '0',
'name' => 'Elution Module',
'description' => '<p>Optimized for for the elution of immunoprecipitated DNA bound to magnetic or agarose beads.</p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '1 unit',
'catalog_number' => 'C01010120',
'old_catalog_number' => 'mc-magme-002',
'sf_code' => 'C01010120-',
'type' => 'RFR',
'search_order' => '04-undefined',
'price_EUR' => '100',
'price_USD' => '150',
'price_GBP' => '95',
'price_JPY' => '15665',
'price_CNY' => '',
'price_AUD' => '375',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 'elution-module-1-unit',
'meta_title' => 'Elution Module',
'meta_keywords' => '',
'meta_description' => 'Elution Module',
'modified' => '2015-07-16 16:09:35',
'created' => '2015-06-29 14:08:20',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(),
'Related' => array(),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
)
)
$language = 'cn'
$meta_keywords = ''
$meta_description = 'Elution Module'
$meta_title = 'Elution Module'
$product = array(
'Product' => array(
'id' => '1855',
'antibody_id' => '0',
'name' => 'Elution Module',
'description' => '<p>Optimized for for the elution of immunoprecipitated DNA bound to magnetic or agarose beads.</p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '1 unit',
'catalog_number' => 'C01010120',
'old_catalog_number' => 'mc-magme-002',
'sf_code' => 'C01010120-',
'type' => 'RFR',
'search_order' => '04-undefined',
'price_EUR' => '100',
'price_USD' => '150',
'price_GBP' => '95',
'price_JPY' => '15665',
'price_CNY' => '',
'price_AUD' => '375',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 'elution-module-1-unit',
'meta_title' => 'Elution Module',
'meta_keywords' => '',
'meta_description' => 'Elution Module',
'modified' => '2015-07-16 16:09:35',
'created' => '2015-06-29 14:08:20',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(),
'Related' => array(),
'Application' => array(
(int) 0 => array(
'id' => '10',
'position' => '10',
'parent_id' => '2',
'name' => 'ChIP-qPCR',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns text-justify">
<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
<li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li>
</ol>
</div>
</div>
<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div>
</div>
</div>
</div>
</div>',
'in_footer' => false,
'in_menu' => true,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr',
'meta_keywords' => 'Chromatin immunoprecipitation,ChIP Quantitative PCR,polymerase chain reaction (PCR)',
'meta_description' => 'Diagenode's ChIP qPCR kits can be used to quantify enriched DNA after chromatin immunoprecipitation. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of',
'meta_title' => 'ChIP Quantitative PCR (ChIP-qPCR) | Diagenode',
'modified' => '2018-01-09 16:46:56',
'created' => '2014-12-11 00:22:08',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '64',
'position' => '7',
'parent_id' => '14',
'name' => 'Elution',
'description' => '',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'hide' => false,
'all_format' => false,
'is_antibody' => false,
'slug' => 'elution',
'cookies_tag_id' => null,
'meta_keywords' => 'Elution Module,immunoprecipitated DNA,agarose beads,magnetic beads,diagenode',
'meta_description' => 'Diagenode offers Elution Module for optimizing the elution of immunoprecipitated DNA bound to magnetic or agarose beads.',
'meta_title' => 'Elution for DNA and RNA purification | Diagenode',
'modified' => '2016-02-19 16:00:22',
'created' => '2015-07-08 10:46:17',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '68',
'name' => 'DNA Elution Module',
'description' => '<div class="page" title="Page 4">
<div class="section">
<div class="layoutArea">
<div class="column">
<p><span>The DNA elution module allows the elution of chromatin and DNA from immunoprecipitated material after ChIP and MeDIP respectively. Afterwards, you can proceed to the DNA purification using columns or carrying out a phenol/ chloroform/isomayl alcohol extraction. </span></p>
</div>
</div>
</div>
</div>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/Elution_Module_manual.pdf',
'slug' => 'elution-module-manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-09-01 11:55:17',
'created' => '2015-07-07 11:47:43',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(
(int) 0 => array(
'id' => '1',
'name' => 'Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor<sup>®</sup> Standard, Plus or Pico',
'description' => '<p>This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chromatin can be used for chromatin immunoprecipitation assays using Diagenode’s iDeal ChIP-seq kit (C01010051). The following protocol is optimized for 30-40 mg of tissue allowing up to 18 ChIP samples (1.5-2 mg of tissue per sample). However, the exact amount of tissue needed for ChIP may vary depending on protein abundance, antibody affinity etc. and should be determined for each tissue type.</p>',
'image_id' => '222',
'type' => 'Protocol',
'url' => 'files/protocols/Chromatin_shearing_from_tissue_protocol.pdf',
'slug' => 'chromatin-shearing-from-tissue-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-09-29 22:10:32',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
)
),
'Publication' => array(
(int) 0 => array(
'id' => '3630',
'name' => 'Hyper-Editing of Cell-Cycle Regulatory and Tumor Suppressor RNA Promotes Malignant Progenitor Propagation.',
'authors' => 'Jiang Q, Isquith J, Zipeto MA, Diep RH, Pham J, Delos Santos N, Reynoso E, Chau J, Leu H, Lazzari E, Melese E, Ma W, Fang R, Minden M, Morris S, Ren B, Pineda G, Holm F, Jamieson C',
'description' => '<p>Adenosine deaminase associated with RNA1 (ADAR1) deregulation contributes to therapeutic resistance in many malignancies. Here we show that ADAR1-induced hyper-editing in normal human hematopoietic progenitors impairs miR-26a maturation, which represses CDKN1A expression indirectly via EZH2, thereby accelerating cell-cycle transit. However, in blast crisis chronic myeloid leukemia progenitors, loss of EZH2 expression and increased CDKN1A oppose cell-cycle transit. Moreover, A-to-I editing of both the MDM2 regulatory microRNA and its binding site within the 3' UTR region stabilizes MDM2 transcripts, thereby enhancing blast crisis progenitor propagation. These data reveal a dual mechanism governing malignant transformation of progenitors that is predicated on hyper-editing of cell-cycle-regulatory miRNAs and the 3' UTR binding site of tumor suppressor miRNAs.</p>',
'date' => '2019-01-14',
'pmid' => 'http://www.pubmed.gov/30612940',
'doi' => '10.1016/j.ccell.2018.11.017',
'modified' => '2019-05-08 12:25:16',
'created' => '2019-04-25 11:11:44',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '606',
'name' => 'Elution Module SDS GB en',
'language' => 'en',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-GB-en-1_0.pdf',
'countries' => 'GB',
'modified' => '2020-07-01 15:35:16',
'created' => '2020-07-01 15:35:16',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '608',
'name' => 'Elution Module SDS US en',
'language' => 'en',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-US-en-1_0.pdf',
'countries' => 'US',
'modified' => '2020-07-01 15:36:01',
'created' => '2020-07-01 15:36:01',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '603',
'name' => 'Elution Module SDS DE de',
'language' => 'de',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-DE-de-1_0.pdf',
'countries' => 'DE',
'modified' => '2020-07-01 15:34:04',
'created' => '2020-07-01 15:34:04',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '607',
'name' => 'Elution Module SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-JP-ja-1_1.pdf',
'countries' => 'JP',
'modified' => '2020-07-01 15:35:39',
'created' => '2020-07-01 15:35:39',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '602',
'name' => 'Elution Module SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-BE-nl-1_0.pdf',
'countries' => 'BE',
'modified' => '2020-07-01 15:33:26',
'created' => '2020-07-01 15:33:26',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '601',
'name' => 'Elution Module SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-BE-fr-1_0.pdf',
'countries' => 'BE',
'modified' => '2020-07-01 15:32:52',
'created' => '2020-07-01 15:32:52',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '605',
'name' => 'Elution Module SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-FR-fr-1_0.pdf',
'countries' => 'FR',
'modified' => '2020-07-01 15:34:55',
'created' => '2020-07-01 15:34:55',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '604',
'name' => 'Elution Module SDS ES es',
'language' => 'es',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-ES-es-1_0.pdf',
'countries' => 'ES',
'modified' => '2020-07-01 15:34:32',
'created' => '2020-07-01 15:34:32',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = '<br/><small><span style="color:#CCC">(mc-magme-002)</span></small>'
$country_code = 'US'
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$protocol = array(
'id' => '1',
'name' => 'Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor<sup>®</sup> Standard, Plus or Pico',
'description' => '<p>This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chromatin can be used for chromatin immunoprecipitation assays using Diagenode’s iDeal ChIP-seq kit (C01010051). The following protocol is optimized for 30-40 mg of tissue allowing up to 18 ChIP samples (1.5-2 mg of tissue per sample). However, the exact amount of tissue needed for ChIP may vary depending on protein abundance, antibody affinity etc. and should be determined for each tissue type.</p>',
'image_id' => '222',
'type' => 'Protocol',
'url' => 'files/protocols/Chromatin_shearing_from_tissue_protocol.pdf',
'slug' => 'chromatin-shearing-from-tissue-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-09-29 22:10:32',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
'id' => '39',
'product_id' => '1855',
'protocol_id' => '1'
)
)
$application = array(
'id' => '10',
'position' => '10',
'parent_id' => '2',
'name' => 'ChIP-qPCR',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns text-justify">
<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
<li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li>
</ol>
</div>
</div>
<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div>
</div>
</div>
</div>
</div>',
'in_footer' => false,
'in_menu' => true,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr',
'meta_keywords' => 'Chromatin immunoprecipitation,ChIP Quantitative PCR,polymerase chain reaction (PCR)',
'meta_description' => 'Diagenode's ChIP qPCR kits can be used to quantify enriched DNA after chromatin immunoprecipitation. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of',
'meta_title' => 'ChIP Quantitative PCR (ChIP-qPCR) | Diagenode',
'modified' => '2018-01-09 16:46:56',
'created' => '2014-12-11 00:22:08',
'ProductsApplication' => array(
'id' => '2134',
'product_id' => '1855',
'application_id' => '10'
)
)
$slugs = array(
(int) 0 => 'chip-qpcr'
)
$applications = array(
'id' => '10',
'position' => '10',
'parent_id' => '2',
'name' => 'ChIP-qPCR',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns text-justify">
<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="../categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
<li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li>
</ol>
</div>
</div>
<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
<div class="small-6 medium-6 large-6 columns"><a href="../pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div>
</div>
</div>
</div>
</div>',
'in_footer' => false,
'in_menu' => true,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr',
'meta_keywords' => 'Chromatin immunoprecipitation,ChIP Quantitative PCR,polymerase chain reaction (PCR)',
'meta_description' => 'Diagenode's ChIP qPCR kits can be used to quantify enriched DNA after chromatin immunoprecipitation. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of',
'meta_title' => 'ChIP Quantitative PCR (ChIP-qPCR) | Diagenode',
'modified' => '2018-01-09 16:46:56',
'created' => '2014-12-11 00:22:08',
'locale' => 'zho'
)
$description = '<div class="row">
<div class="small-12 medium-12 large-12 columns text-justify">
<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="../categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
<li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li>
</ol>
</div>
</div>
<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
<div class="small-6 medium-6 large-6 columns"><a href="../pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div>
</div>
</div>
</div>
</div>'
$name = 'ChIP-qPCR'
$document = array(
'id' => '68',
'name' => 'DNA Elution Module',
'description' => '<div class="page" title="Page 4">
<div class="section">
<div class="layoutArea">
<div class="column">
<p><span>The DNA elution module allows the elution of chromatin and DNA from immunoprecipitated material after ChIP and MeDIP respectively. Afterwards, you can proceed to the DNA purification using columns or carrying out a phenol/ chloroform/isomayl alcohol extraction. </span></p>
</div>
</div>
</div>
</div>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/Elution_Module_manual.pdf',
'slug' => 'elution-module-manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-09-01 11:55:17',
'created' => '2015-07-07 11:47:43',
'ProductsDocument' => array(
'id' => '280',
'product_id' => '1855',
'document_id' => '68'
)
)
$sds = array(
'id' => '604',
'name' => 'Elution Module SDS ES es',
'language' => 'es',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-ES-es-1_0.pdf',
'countries' => 'ES',
'modified' => '2020-07-01 15:34:32',
'created' => '2020-07-01 15:34:32',
'ProductsSafetySheet' => array(
'id' => '1139',
'product_id' => '1855',
'safety_sheet_id' => '604'
)
)
$publication = array(
'id' => '3630',
'name' => 'Hyper-Editing of Cell-Cycle Regulatory and Tumor Suppressor RNA Promotes Malignant Progenitor Propagation.',
'authors' => 'Jiang Q, Isquith J, Zipeto MA, Diep RH, Pham J, Delos Santos N, Reynoso E, Chau J, Leu H, Lazzari E, Melese E, Ma W, Fang R, Minden M, Morris S, Ren B, Pineda G, Holm F, Jamieson C',
'description' => '<p>Adenosine deaminase associated with RNA1 (ADAR1) deregulation contributes to therapeutic resistance in many malignancies. Here we show that ADAR1-induced hyper-editing in normal human hematopoietic progenitors impairs miR-26a maturation, which represses CDKN1A expression indirectly via EZH2, thereby accelerating cell-cycle transit. However, in blast crisis chronic myeloid leukemia progenitors, loss of EZH2 expression and increased CDKN1A oppose cell-cycle transit. Moreover, A-to-I editing of both the MDM2 regulatory microRNA and its binding site within the 3' UTR region stabilizes MDM2 transcripts, thereby enhancing blast crisis progenitor propagation. These data reveal a dual mechanism governing malignant transformation of progenitors that is predicated on hyper-editing of cell-cycle-regulatory miRNAs and the 3' UTR binding site of tumor suppressor miRNAs.</p>',
'date' => '2019-01-14',
'pmid' => 'http://www.pubmed.gov/30612940',
'doi' => '10.1016/j.ccell.2018.11.017',
'modified' => '2019-05-08 12:25:16',
'created' => '2019-04-25 11:11:44',
'ProductsPublication' => array(
'id' => '3574',
'product_id' => '1855',
'publication_id' => '3630'
)
)
$externalLink = ' <a href="http://www.pubmed.gov/30612940" target="_blank"><i class="fa fa-external-link"></i></a>'
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'cn',
'meta_keywords' => '',
'meta_description' => 'Elution Module',
'meta_title' => 'Elution Module',
'product' => array(
'Product' => array(
'id' => '1855',
'antibody_id' => '0',
'name' => 'Elution Module',
'description' => '<p>Optimized for for the elution of immunoprecipitated DNA bound to magnetic or agarose beads.</p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '1 unit',
'catalog_number' => 'C01010120',
'old_catalog_number' => 'mc-magme-002',
'sf_code' => 'C01010120-',
'type' => 'RFR',
'search_order' => '04-undefined',
'price_EUR' => '100',
'price_USD' => '150',
'price_GBP' => '95',
'price_JPY' => '15665',
'price_CNY' => '',
'price_AUD' => '375',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 'elution-module-1-unit',
'meta_title' => 'Elution Module',
'meta_keywords' => '',
'meta_description' => 'Elution Module',
'modified' => '2015-07-16 16:09:35',
'created' => '2015-06-29 14:08:20',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(),
'Related' => array(),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
)
)
$language = 'cn'
$meta_keywords = ''
$meta_description = 'Elution Module'
$meta_title = 'Elution Module'
$product = array(
'Product' => array(
'id' => '1855',
'antibody_id' => '0',
'name' => 'Elution Module',
'description' => '<p>Optimized for for the elution of immunoprecipitated DNA bound to magnetic or agarose beads.</p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '1 unit',
'catalog_number' => 'C01010120',
'old_catalog_number' => 'mc-magme-002',
'sf_code' => 'C01010120-',
'type' => 'RFR',
'search_order' => '04-undefined',
'price_EUR' => '100',
'price_USD' => '150',
'price_GBP' => '95',
'price_JPY' => '15665',
'price_CNY' => '',
'price_AUD' => '375',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 'elution-module-1-unit',
'meta_title' => 'Elution Module',
'meta_keywords' => '',
'meta_description' => 'Elution Module',
'modified' => '2015-07-16 16:09:35',
'created' => '2015-06-29 14:08:20',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(),
'Related' => array(),
'Application' => array(
(int) 0 => array(
'id' => '10',
'position' => '10',
'parent_id' => '2',
'name' => 'ChIP-qPCR',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns text-justify">
<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
<li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li>
</ol>
</div>
</div>
<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div>
</div>
</div>
</div>
</div>',
'in_footer' => false,
'in_menu' => true,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr',
'meta_keywords' => 'Chromatin immunoprecipitation,ChIP Quantitative PCR,polymerase chain reaction (PCR)',
'meta_description' => 'Diagenode's ChIP qPCR kits can be used to quantify enriched DNA after chromatin immunoprecipitation. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of',
'meta_title' => 'ChIP Quantitative PCR (ChIP-qPCR) | Diagenode',
'modified' => '2018-01-09 16:46:56',
'created' => '2014-12-11 00:22:08',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '64',
'position' => '7',
'parent_id' => '14',
'name' => 'Elution',
'description' => '',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'hide' => false,
'all_format' => false,
'is_antibody' => false,
'slug' => 'elution',
'cookies_tag_id' => null,
'meta_keywords' => 'Elution Module,immunoprecipitated DNA,agarose beads,magnetic beads,diagenode',
'meta_description' => 'Diagenode offers Elution Module for optimizing the elution of immunoprecipitated DNA bound to magnetic or agarose beads.',
'meta_title' => 'Elution for DNA and RNA purification | Diagenode',
'modified' => '2016-02-19 16:00:22',
'created' => '2015-07-08 10:46:17',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '68',
'name' => 'DNA Elution Module',
'description' => '<div class="page" title="Page 4">
<div class="section">
<div class="layoutArea">
<div class="column">
<p><span>The DNA elution module allows the elution of chromatin and DNA from immunoprecipitated material after ChIP and MeDIP respectively. Afterwards, you can proceed to the DNA purification using columns or carrying out a phenol/ chloroform/isomayl alcohol extraction. </span></p>
</div>
</div>
</div>
</div>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/Elution_Module_manual.pdf',
'slug' => 'elution-module-manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-09-01 11:55:17',
'created' => '2015-07-07 11:47:43',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(
(int) 0 => array(
'id' => '1',
'name' => 'Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor<sup>®</sup> Standard, Plus or Pico',
'description' => '<p>This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chromatin can be used for chromatin immunoprecipitation assays using Diagenode’s iDeal ChIP-seq kit (C01010051). The following protocol is optimized for 30-40 mg of tissue allowing up to 18 ChIP samples (1.5-2 mg of tissue per sample). However, the exact amount of tissue needed for ChIP may vary depending on protein abundance, antibody affinity etc. and should be determined for each tissue type.</p>',
'image_id' => '222',
'type' => 'Protocol',
'url' => 'files/protocols/Chromatin_shearing_from_tissue_protocol.pdf',
'slug' => 'chromatin-shearing-from-tissue-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-09-29 22:10:32',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
)
),
'Publication' => array(
(int) 0 => array(
'id' => '3630',
'name' => 'Hyper-Editing of Cell-Cycle Regulatory and Tumor Suppressor RNA Promotes Malignant Progenitor Propagation.',
'authors' => 'Jiang Q, Isquith J, Zipeto MA, Diep RH, Pham J, Delos Santos N, Reynoso E, Chau J, Leu H, Lazzari E, Melese E, Ma W, Fang R, Minden M, Morris S, Ren B, Pineda G, Holm F, Jamieson C',
'description' => '<p>Adenosine deaminase associated with RNA1 (ADAR1) deregulation contributes to therapeutic resistance in many malignancies. Here we show that ADAR1-induced hyper-editing in normal human hematopoietic progenitors impairs miR-26a maturation, which represses CDKN1A expression indirectly via EZH2, thereby accelerating cell-cycle transit. However, in blast crisis chronic myeloid leukemia progenitors, loss of EZH2 expression and increased CDKN1A oppose cell-cycle transit. Moreover, A-to-I editing of both the MDM2 regulatory microRNA and its binding site within the 3' UTR region stabilizes MDM2 transcripts, thereby enhancing blast crisis progenitor propagation. These data reveal a dual mechanism governing malignant transformation of progenitors that is predicated on hyper-editing of cell-cycle-regulatory miRNAs and the 3' UTR binding site of tumor suppressor miRNAs.</p>',
'date' => '2019-01-14',
'pmid' => 'http://www.pubmed.gov/30612940',
'doi' => '10.1016/j.ccell.2018.11.017',
'modified' => '2019-05-08 12:25:16',
'created' => '2019-04-25 11:11:44',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '606',
'name' => 'Elution Module SDS GB en',
'language' => 'en',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-GB-en-1_0.pdf',
'countries' => 'GB',
'modified' => '2020-07-01 15:35:16',
'created' => '2020-07-01 15:35:16',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '608',
'name' => 'Elution Module SDS US en',
'language' => 'en',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-US-en-1_0.pdf',
'countries' => 'US',
'modified' => '2020-07-01 15:36:01',
'created' => '2020-07-01 15:36:01',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '603',
'name' => 'Elution Module SDS DE de',
'language' => 'de',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-DE-de-1_0.pdf',
'countries' => 'DE',
'modified' => '2020-07-01 15:34:04',
'created' => '2020-07-01 15:34:04',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '607',
'name' => 'Elution Module SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-JP-ja-1_1.pdf',
'countries' => 'JP',
'modified' => '2020-07-01 15:35:39',
'created' => '2020-07-01 15:35:39',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '602',
'name' => 'Elution Module SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-BE-nl-1_0.pdf',
'countries' => 'BE',
'modified' => '2020-07-01 15:33:26',
'created' => '2020-07-01 15:33:26',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '601',
'name' => 'Elution Module SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-BE-fr-1_0.pdf',
'countries' => 'BE',
'modified' => '2020-07-01 15:32:52',
'created' => '2020-07-01 15:32:52',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '605',
'name' => 'Elution Module SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-FR-fr-1_0.pdf',
'countries' => 'FR',
'modified' => '2020-07-01 15:34:55',
'created' => '2020-07-01 15:34:55',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '604',
'name' => 'Elution Module SDS ES es',
'language' => 'es',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-ES-es-1_0.pdf',
'countries' => 'ES',
'modified' => '2020-07-01 15:34:32',
'created' => '2020-07-01 15:34:32',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = '<br/><small><span style="color:#CCC">(mc-magme-002)</span></small>'
$country_code = 'US'
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$protocol = array(
'id' => '1',
'name' => 'Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor<sup>®</sup> Standard, Plus or Pico',
'description' => '<p>This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chromatin can be used for chromatin immunoprecipitation assays using Diagenode’s iDeal ChIP-seq kit (C01010051). The following protocol is optimized for 30-40 mg of tissue allowing up to 18 ChIP samples (1.5-2 mg of tissue per sample). However, the exact amount of tissue needed for ChIP may vary depending on protein abundance, antibody affinity etc. and should be determined for each tissue type.</p>',
'image_id' => '222',
'type' => 'Protocol',
'url' => 'files/protocols/Chromatin_shearing_from_tissue_protocol.pdf',
'slug' => 'chromatin-shearing-from-tissue-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-09-29 22:10:32',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
'id' => '39',
'product_id' => '1855',
'protocol_id' => '1'
)
)
$application = array(
'id' => '10',
'position' => '10',
'parent_id' => '2',
'name' => 'ChIP-qPCR',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns text-justify">
<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
<li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li>
</ol>
</div>
</div>
<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div>
</div>
</div>
</div>
</div>',
'in_footer' => false,
'in_menu' => true,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr',
'meta_keywords' => 'Chromatin immunoprecipitation,ChIP Quantitative PCR,polymerase chain reaction (PCR)',
'meta_description' => 'Diagenode's ChIP qPCR kits can be used to quantify enriched DNA after chromatin immunoprecipitation. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of',
'meta_title' => 'ChIP Quantitative PCR (ChIP-qPCR) | Diagenode',
'modified' => '2018-01-09 16:46:56',
'created' => '2014-12-11 00:22:08',
'ProductsApplication' => array(
'id' => '2134',
'product_id' => '1855',
'application_id' => '10'
)
)
$slugs = array(
(int) 0 => 'chip-qpcr'
)
$applications = array(
'id' => '10',
'position' => '10',
'parent_id' => '2',
'name' => 'ChIP-qPCR',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns text-justify">
<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="../categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
<li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li>
</ol>
</div>
</div>
<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
<div class="small-6 medium-6 large-6 columns"><a href="../pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div>
</div>
</div>
</div>
</div>',
'in_footer' => false,
'in_menu' => true,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr',
'meta_keywords' => 'Chromatin immunoprecipitation,ChIP Quantitative PCR,polymerase chain reaction (PCR)',
'meta_description' => 'Diagenode's ChIP qPCR kits can be used to quantify enriched DNA after chromatin immunoprecipitation. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of',
'meta_title' => 'ChIP Quantitative PCR (ChIP-qPCR) | Diagenode',
'modified' => '2018-01-09 16:46:56',
'created' => '2014-12-11 00:22:08',
'locale' => 'zho'
)
$description = '<div class="row">
<div class="small-12 medium-12 large-12 columns text-justify">
<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="../categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
<li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li>
</ol>
</div>
</div>
<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
<div class="small-6 medium-6 large-6 columns"><a href="../pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div>
</div>
</div>
</div>
</div>'
$name = 'ChIP-qPCR'
$document = array(
'id' => '68',
'name' => 'DNA Elution Module',
'description' => '<div class="page" title="Page 4">
<div class="section">
<div class="layoutArea">
<div class="column">
<p><span>The DNA elution module allows the elution of chromatin and DNA from immunoprecipitated material after ChIP and MeDIP respectively. Afterwards, you can proceed to the DNA purification using columns or carrying out a phenol/ chloroform/isomayl alcohol extraction. </span></p>
</div>
</div>
</div>
</div>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/Elution_Module_manual.pdf',
'slug' => 'elution-module-manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-09-01 11:55:17',
'created' => '2015-07-07 11:47:43',
'ProductsDocument' => array(
'id' => '280',
'product_id' => '1855',
'document_id' => '68'
)
)
$sds = array(
'id' => '604',
'name' => 'Elution Module SDS ES es',
'language' => 'es',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-ES-es-1_0.pdf',
'countries' => 'ES',
'modified' => '2020-07-01 15:34:32',
'created' => '2020-07-01 15:34:32',
'ProductsSafetySheet' => array(
'id' => '1139',
'product_id' => '1855',
'safety_sheet_id' => '604'
)
)
$publication = array(
'id' => '3630',
'name' => 'Hyper-Editing of Cell-Cycle Regulatory and Tumor Suppressor RNA Promotes Malignant Progenitor Propagation.',
'authors' => 'Jiang Q, Isquith J, Zipeto MA, Diep RH, Pham J, Delos Santos N, Reynoso E, Chau J, Leu H, Lazzari E, Melese E, Ma W, Fang R, Minden M, Morris S, Ren B, Pineda G, Holm F, Jamieson C',
'description' => '<p>Adenosine deaminase associated with RNA1 (ADAR1) deregulation contributes to therapeutic resistance in many malignancies. Here we show that ADAR1-induced hyper-editing in normal human hematopoietic progenitors impairs miR-26a maturation, which represses CDKN1A expression indirectly via EZH2, thereby accelerating cell-cycle transit. However, in blast crisis chronic myeloid leukemia progenitors, loss of EZH2 expression and increased CDKN1A oppose cell-cycle transit. Moreover, A-to-I editing of both the MDM2 regulatory microRNA and its binding site within the 3' UTR region stabilizes MDM2 transcripts, thereby enhancing blast crisis progenitor propagation. These data reveal a dual mechanism governing malignant transformation of progenitors that is predicated on hyper-editing of cell-cycle-regulatory miRNAs and the 3' UTR binding site of tumor suppressor miRNAs.</p>',
'date' => '2019-01-14',
'pmid' => 'http://www.pubmed.gov/30612940',
'doi' => '10.1016/j.ccell.2018.11.017',
'modified' => '2019-05-08 12:25:16',
'created' => '2019-04-25 11:11:44',
'ProductsPublication' => array(
'id' => '3574',
'product_id' => '1855',
'publication_id' => '3630'
)
)
$externalLink = ' <a href="http://www.pubmed.gov/30612940" target="_blank"><i class="fa fa-external-link"></i></a>'
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'cn',
'meta_keywords' => '',
'meta_description' => 'Elution Module',
'meta_title' => 'Elution Module',
'product' => array(
'Product' => array(
'id' => '1855',
'antibody_id' => '0',
'name' => 'Elution Module',
'description' => '<p>Optimized for for the elution of immunoprecipitated DNA bound to magnetic or agarose beads.</p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '1 unit',
'catalog_number' => 'C01010120',
'old_catalog_number' => 'mc-magme-002',
'sf_code' => 'C01010120-',
'type' => 'RFR',
'search_order' => '04-undefined',
'price_EUR' => '100',
'price_USD' => '150',
'price_GBP' => '95',
'price_JPY' => '15665',
'price_CNY' => '',
'price_AUD' => '375',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 'elution-module-1-unit',
'meta_title' => 'Elution Module',
'meta_keywords' => '',
'meta_description' => 'Elution Module',
'modified' => '2015-07-16 16:09:35',
'created' => '2015-06-29 14:08:20',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(),
'Related' => array(),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
)
)
$language = 'cn'
$meta_keywords = ''
$meta_description = 'Elution Module'
$meta_title = 'Elution Module'
$product = array(
'Product' => array(
'id' => '1855',
'antibody_id' => '0',
'name' => 'Elution Module',
'description' => '<p>Optimized for for the elution of immunoprecipitated DNA bound to magnetic or agarose beads.</p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '1 unit',
'catalog_number' => 'C01010120',
'old_catalog_number' => 'mc-magme-002',
'sf_code' => 'C01010120-',
'type' => 'RFR',
'search_order' => '04-undefined',
'price_EUR' => '100',
'price_USD' => '150',
'price_GBP' => '95',
'price_JPY' => '15665',
'price_CNY' => '',
'price_AUD' => '375',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 'elution-module-1-unit',
'meta_title' => 'Elution Module',
'meta_keywords' => '',
'meta_description' => 'Elution Module',
'modified' => '2015-07-16 16:09:35',
'created' => '2015-06-29 14:08:20',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(),
'Related' => array(),
'Application' => array(
(int) 0 => array(
'id' => '10',
'position' => '10',
'parent_id' => '2',
'name' => 'ChIP-qPCR',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns text-justify">
<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
<li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li>
</ol>
</div>
</div>
<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div>
</div>
</div>
</div>
</div>',
'in_footer' => false,
'in_menu' => true,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr',
'meta_keywords' => 'Chromatin immunoprecipitation,ChIP Quantitative PCR,polymerase chain reaction (PCR)',
'meta_description' => 'Diagenode's ChIP qPCR kits can be used to quantify enriched DNA after chromatin immunoprecipitation. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of',
'meta_title' => 'ChIP Quantitative PCR (ChIP-qPCR) | Diagenode',
'modified' => '2018-01-09 16:46:56',
'created' => '2014-12-11 00:22:08',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '64',
'position' => '7',
'parent_id' => '14',
'name' => 'Elution',
'description' => '',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'hide' => false,
'all_format' => false,
'is_antibody' => false,
'slug' => 'elution',
'cookies_tag_id' => null,
'meta_keywords' => 'Elution Module,immunoprecipitated DNA,agarose beads,magnetic beads,diagenode',
'meta_description' => 'Diagenode offers Elution Module for optimizing the elution of immunoprecipitated DNA bound to magnetic or agarose beads.',
'meta_title' => 'Elution for DNA and RNA purification | Diagenode',
'modified' => '2016-02-19 16:00:22',
'created' => '2015-07-08 10:46:17',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '68',
'name' => 'DNA Elution Module',
'description' => '<div class="page" title="Page 4">
<div class="section">
<div class="layoutArea">
<div class="column">
<p><span>The DNA elution module allows the elution of chromatin and DNA from immunoprecipitated material after ChIP and MeDIP respectively. Afterwards, you can proceed to the DNA purification using columns or carrying out a phenol/ chloroform/isomayl alcohol extraction. </span></p>
</div>
</div>
</div>
</div>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/Elution_Module_manual.pdf',
'slug' => 'elution-module-manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-09-01 11:55:17',
'created' => '2015-07-07 11:47:43',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(
(int) 0 => array(
'id' => '1',
'name' => 'Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor<sup>®</sup> Standard, Plus or Pico',
'description' => '<p>This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chromatin can be used for chromatin immunoprecipitation assays using Diagenode’s iDeal ChIP-seq kit (C01010051). The following protocol is optimized for 30-40 mg of tissue allowing up to 18 ChIP samples (1.5-2 mg of tissue per sample). However, the exact amount of tissue needed for ChIP may vary depending on protein abundance, antibody affinity etc. and should be determined for each tissue type.</p>',
'image_id' => '222',
'type' => 'Protocol',
'url' => 'files/protocols/Chromatin_shearing_from_tissue_protocol.pdf',
'slug' => 'chromatin-shearing-from-tissue-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-09-29 22:10:32',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
)
),
'Publication' => array(
(int) 0 => array(
'id' => '3630',
'name' => 'Hyper-Editing of Cell-Cycle Regulatory and Tumor Suppressor RNA Promotes Malignant Progenitor Propagation.',
'authors' => 'Jiang Q, Isquith J, Zipeto MA, Diep RH, Pham J, Delos Santos N, Reynoso E, Chau J, Leu H, Lazzari E, Melese E, Ma W, Fang R, Minden M, Morris S, Ren B, Pineda G, Holm F, Jamieson C',
'description' => '<p>Adenosine deaminase associated with RNA1 (ADAR1) deregulation contributes to therapeutic resistance in many malignancies. Here we show that ADAR1-induced hyper-editing in normal human hematopoietic progenitors impairs miR-26a maturation, which represses CDKN1A expression indirectly via EZH2, thereby accelerating cell-cycle transit. However, in blast crisis chronic myeloid leukemia progenitors, loss of EZH2 expression and increased CDKN1A oppose cell-cycle transit. Moreover, A-to-I editing of both the MDM2 regulatory microRNA and its binding site within the 3' UTR region stabilizes MDM2 transcripts, thereby enhancing blast crisis progenitor propagation. These data reveal a dual mechanism governing malignant transformation of progenitors that is predicated on hyper-editing of cell-cycle-regulatory miRNAs and the 3' UTR binding site of tumor suppressor miRNAs.</p>',
'date' => '2019-01-14',
'pmid' => 'http://www.pubmed.gov/30612940',
'doi' => '10.1016/j.ccell.2018.11.017',
'modified' => '2019-05-08 12:25:16',
'created' => '2019-04-25 11:11:44',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '606',
'name' => 'Elution Module SDS GB en',
'language' => 'en',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-GB-en-1_0.pdf',
'countries' => 'GB',
'modified' => '2020-07-01 15:35:16',
'created' => '2020-07-01 15:35:16',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '608',
'name' => 'Elution Module SDS US en',
'language' => 'en',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-US-en-1_0.pdf',
'countries' => 'US',
'modified' => '2020-07-01 15:36:01',
'created' => '2020-07-01 15:36:01',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '603',
'name' => 'Elution Module SDS DE de',
'language' => 'de',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-DE-de-1_0.pdf',
'countries' => 'DE',
'modified' => '2020-07-01 15:34:04',
'created' => '2020-07-01 15:34:04',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '607',
'name' => 'Elution Module SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-JP-ja-1_1.pdf',
'countries' => 'JP',
'modified' => '2020-07-01 15:35:39',
'created' => '2020-07-01 15:35:39',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '602',
'name' => 'Elution Module SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-BE-nl-1_0.pdf',
'countries' => 'BE',
'modified' => '2020-07-01 15:33:26',
'created' => '2020-07-01 15:33:26',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '601',
'name' => 'Elution Module SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-BE-fr-1_0.pdf',
'countries' => 'BE',
'modified' => '2020-07-01 15:32:52',
'created' => '2020-07-01 15:32:52',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '605',
'name' => 'Elution Module SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-FR-fr-1_0.pdf',
'countries' => 'FR',
'modified' => '2020-07-01 15:34:55',
'created' => '2020-07-01 15:34:55',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '604',
'name' => 'Elution Module SDS ES es',
'language' => 'es',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-ES-es-1_0.pdf',
'countries' => 'ES',
'modified' => '2020-07-01 15:34:32',
'created' => '2020-07-01 15:34:32',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = '<br/><small><span style="color:#CCC">(mc-magme-002)</span></small>'
$country_code = 'US'
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$protocol = array(
'id' => '1',
'name' => 'Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor<sup>®</sup> Standard, Plus or Pico',
'description' => '<p>This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chromatin can be used for chromatin immunoprecipitation assays using Diagenode’s iDeal ChIP-seq kit (C01010051). The following protocol is optimized for 30-40 mg of tissue allowing up to 18 ChIP samples (1.5-2 mg of tissue per sample). However, the exact amount of tissue needed for ChIP may vary depending on protein abundance, antibody affinity etc. and should be determined for each tissue type.</p>',
'image_id' => '222',
'type' => 'Protocol',
'url' => 'files/protocols/Chromatin_shearing_from_tissue_protocol.pdf',
'slug' => 'chromatin-shearing-from-tissue-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-09-29 22:10:32',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
'id' => '39',
'product_id' => '1855',
'protocol_id' => '1'
)
)
$application = array(
'id' => '10',
'position' => '10',
'parent_id' => '2',
'name' => 'ChIP-qPCR',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns text-justify">
<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
<li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li>
</ol>
</div>
</div>
<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div>
</div>
</div>
</div>
</div>',
'in_footer' => false,
'in_menu' => true,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr',
'meta_keywords' => 'Chromatin immunoprecipitation,ChIP Quantitative PCR,polymerase chain reaction (PCR)',
'meta_description' => 'Diagenode's ChIP qPCR kits can be used to quantify enriched DNA after chromatin immunoprecipitation. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of',
'meta_title' => 'ChIP Quantitative PCR (ChIP-qPCR) | Diagenode',
'modified' => '2018-01-09 16:46:56',
'created' => '2014-12-11 00:22:08',
'ProductsApplication' => array(
'id' => '2134',
'product_id' => '1855',
'application_id' => '10'
)
)
$slugs = array(
(int) 0 => 'chip-qpcr'
)
$applications = array(
'id' => '10',
'position' => '10',
'parent_id' => '2',
'name' => 'ChIP-qPCR',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns text-justify">
<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="../categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
<li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li>
</ol>
</div>
</div>
<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
<div class="small-6 medium-6 large-6 columns"><a href="../pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div>
</div>
</div>
</div>
</div>',
'in_footer' => false,
'in_menu' => true,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr',
'meta_keywords' => 'Chromatin immunoprecipitation,ChIP Quantitative PCR,polymerase chain reaction (PCR)',
'meta_description' => 'Diagenode's ChIP qPCR kits can be used to quantify enriched DNA after chromatin immunoprecipitation. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of',
'meta_title' => 'ChIP Quantitative PCR (ChIP-qPCR) | Diagenode',
'modified' => '2018-01-09 16:46:56',
'created' => '2014-12-11 00:22:08',
'locale' => 'zho'
)
$description = '<div class="row">
<div class="small-12 medium-12 large-12 columns text-justify">
<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="../categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
<li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li>
</ol>
</div>
</div>
<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
<div class="small-6 medium-6 large-6 columns"><a href="../pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div>
</div>
</div>
</div>
</div>'
$name = 'ChIP-qPCR'
$document = array(
'id' => '68',
'name' => 'DNA Elution Module',
'description' => '<div class="page" title="Page 4">
<div class="section">
<div class="layoutArea">
<div class="column">
<p><span>The DNA elution module allows the elution of chromatin and DNA from immunoprecipitated material after ChIP and MeDIP respectively. Afterwards, you can proceed to the DNA purification using columns or carrying out a phenol/ chloroform/isomayl alcohol extraction. </span></p>
</div>
</div>
</div>
</div>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/Elution_Module_manual.pdf',
'slug' => 'elution-module-manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-09-01 11:55:17',
'created' => '2015-07-07 11:47:43',
'ProductsDocument' => array(
'id' => '280',
'product_id' => '1855',
'document_id' => '68'
)
)
$sds = array(
'id' => '604',
'name' => 'Elution Module SDS ES es',
'language' => 'es',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-ES-es-1_0.pdf',
'countries' => 'ES',
'modified' => '2020-07-01 15:34:32',
'created' => '2020-07-01 15:34:32',
'ProductsSafetySheet' => array(
'id' => '1139',
'product_id' => '1855',
'safety_sheet_id' => '604'
)
)
$publication = array(
'id' => '3630',
'name' => 'Hyper-Editing of Cell-Cycle Regulatory and Tumor Suppressor RNA Promotes Malignant Progenitor Propagation.',
'authors' => 'Jiang Q, Isquith J, Zipeto MA, Diep RH, Pham J, Delos Santos N, Reynoso E, Chau J, Leu H, Lazzari E, Melese E, Ma W, Fang R, Minden M, Morris S, Ren B, Pineda G, Holm F, Jamieson C',
'description' => '<p>Adenosine deaminase associated with RNA1 (ADAR1) deregulation contributes to therapeutic resistance in many malignancies. Here we show that ADAR1-induced hyper-editing in normal human hematopoietic progenitors impairs miR-26a maturation, which represses CDKN1A expression indirectly via EZH2, thereby accelerating cell-cycle transit. However, in blast crisis chronic myeloid leukemia progenitors, loss of EZH2 expression and increased CDKN1A oppose cell-cycle transit. Moreover, A-to-I editing of both the MDM2 regulatory microRNA and its binding site within the 3' UTR region stabilizes MDM2 transcripts, thereby enhancing blast crisis progenitor propagation. These data reveal a dual mechanism governing malignant transformation of progenitors that is predicated on hyper-editing of cell-cycle-regulatory miRNAs and the 3' UTR binding site of tumor suppressor miRNAs.</p>',
'date' => '2019-01-14',
'pmid' => 'http://www.pubmed.gov/30612940',
'doi' => '10.1016/j.ccell.2018.11.017',
'modified' => '2019-05-08 12:25:16',
'created' => '2019-04-25 11:11:44',
'ProductsPublication' => array(
'id' => '3574',
'product_id' => '1855',
'publication_id' => '3630'
)
)
$externalLink = ' <a href="http://www.pubmed.gov/30612940" target="_blank"><i class="fa fa-external-link"></i></a>'
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
'language' => 'cn',
'meta_keywords' => '',
'meta_description' => 'Elution Module',
'meta_title' => 'Elution Module',
'product' => array(
'Product' => array(
'id' => '1855',
'antibody_id' => '0',
'name' => 'Elution Module',
'description' => '<p>Optimized for for the elution of immunoprecipitated DNA bound to magnetic or agarose beads.</p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '1 unit',
'catalog_number' => 'C01010120',
'old_catalog_number' => 'mc-magme-002',
'sf_code' => 'C01010120-',
'type' => 'RFR',
'search_order' => '04-undefined',
'price_EUR' => '100',
'price_USD' => '150',
'price_GBP' => '95',
'price_JPY' => '15665',
'price_CNY' => '',
'price_AUD' => '375',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 'elution-module-1-unit',
'meta_title' => 'Elution Module',
'meta_keywords' => '',
'meta_description' => 'Elution Module',
'modified' => '2015-07-16 16:09:35',
'created' => '2015-06-29 14:08:20',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(),
'Related' => array(),
'Application' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Category' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Document' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Publication' => array(
(int) 0 => array(
[maximum depth reached]
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
[maximum depth reached]
),
(int) 1 => array(
[maximum depth reached]
),
(int) 2 => array(
[maximum depth reached]
),
(int) 3 => array(
[maximum depth reached]
),
(int) 4 => array(
[maximum depth reached]
),
(int) 5 => array(
[maximum depth reached]
),
(int) 6 => array(
[maximum depth reached]
),
(int) 7 => array(
[maximum depth reached]
)
)
)
)
$language = 'cn'
$meta_keywords = ''
$meta_description = 'Elution Module'
$meta_title = 'Elution Module'
$product = array(
'Product' => array(
'id' => '1855',
'antibody_id' => '0',
'name' => 'Elution Module',
'description' => '<p>Optimized for for the elution of immunoprecipitated DNA bound to magnetic or agarose beads.</p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '1 unit',
'catalog_number' => 'C01010120',
'old_catalog_number' => 'mc-magme-002',
'sf_code' => 'C01010120-',
'type' => 'RFR',
'search_order' => '04-undefined',
'price_EUR' => '100',
'price_USD' => '150',
'price_GBP' => '95',
'price_JPY' => '15665',
'price_CNY' => '',
'price_AUD' => '375',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => 'elution-module-1-unit',
'meta_title' => 'Elution Module',
'meta_keywords' => '',
'meta_description' => 'Elution Module',
'modified' => '2015-07-16 16:09:35',
'created' => '2015-06-29 14:08:20',
'locale' => 'zho'
),
'Antibody' => array(
'host' => '*****',
'id' => null,
'name' => null,
'description' => null,
'clonality' => null,
'isotype' => null,
'lot' => null,
'concentration' => null,
'reactivity' => null,
'type' => null,
'purity' => null,
'classification' => null,
'application_table' => null,
'storage_conditions' => null,
'storage_buffer' => null,
'precautions' => null,
'uniprot_acc' => null,
'slug' => null,
'meta_keywords' => null,
'meta_description' => null,
'modified' => null,
'created' => null,
'select_label' => null
),
'Slave' => array(),
'Group' => array(),
'Related' => array(),
'Application' => array(
(int) 0 => array(
'id' => '10',
'position' => '10',
'parent_id' => '2',
'name' => 'ChIP-qPCR',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns text-justify">
<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
<li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li>
</ol>
</div>
</div>
<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div>
</div>
</div>
</div>
</div>',
'in_footer' => false,
'in_menu' => true,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr',
'meta_keywords' => 'Chromatin immunoprecipitation,ChIP Quantitative PCR,polymerase chain reaction (PCR)',
'meta_description' => 'Diagenode's ChIP qPCR kits can be used to quantify enriched DNA after chromatin immunoprecipitation. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of',
'meta_title' => 'ChIP Quantitative PCR (ChIP-qPCR) | Diagenode',
'modified' => '2018-01-09 16:46:56',
'created' => '2014-12-11 00:22:08',
'ProductsApplication' => array(
[maximum depth reached]
)
)
),
'Category' => array(
(int) 0 => array(
'id' => '64',
'position' => '7',
'parent_id' => '14',
'name' => 'Elution',
'description' => '',
'no_promo' => false,
'in_menu' => false,
'online' => true,
'tabular' => true,
'hide' => false,
'all_format' => false,
'is_antibody' => false,
'slug' => 'elution',
'cookies_tag_id' => null,
'meta_keywords' => 'Elution Module,immunoprecipitated DNA,agarose beads,magnetic beads,diagenode',
'meta_description' => 'Diagenode offers Elution Module for optimizing the elution of immunoprecipitated DNA bound to magnetic or agarose beads.',
'meta_title' => 'Elution for DNA and RNA purification | Diagenode',
'modified' => '2016-02-19 16:00:22',
'created' => '2015-07-08 10:46:17',
'ProductsCategory' => array(
[maximum depth reached]
),
'CookiesTag' => array([maximum depth reached])
)
),
'Document' => array(
(int) 0 => array(
'id' => '68',
'name' => 'DNA Elution Module',
'description' => '<div class="page" title="Page 4">
<div class="section">
<div class="layoutArea">
<div class="column">
<p><span>The DNA elution module allows the elution of chromatin and DNA from immunoprecipitated material after ChIP and MeDIP respectively. Afterwards, you can proceed to the DNA purification using columns or carrying out a phenol/ chloroform/isomayl alcohol extraction. </span></p>
</div>
</div>
</div>
</div>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/Elution_Module_manual.pdf',
'slug' => 'elution-module-manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-09-01 11:55:17',
'created' => '2015-07-07 11:47:43',
'ProductsDocument' => array(
[maximum depth reached]
)
)
),
'Feature' => array(),
'Image' => array(),
'Promotion' => array(),
'Protocol' => array(
(int) 0 => array(
'id' => '1',
'name' => 'Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor<sup>®</sup> Standard, Plus or Pico',
'description' => '<p>This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chromatin can be used for chromatin immunoprecipitation assays using Diagenode’s iDeal ChIP-seq kit (C01010051). The following protocol is optimized for 30-40 mg of tissue allowing up to 18 ChIP samples (1.5-2 mg of tissue per sample). However, the exact amount of tissue needed for ChIP may vary depending on protein abundance, antibody affinity etc. and should be determined for each tissue type.</p>',
'image_id' => '222',
'type' => 'Protocol',
'url' => 'files/protocols/Chromatin_shearing_from_tissue_protocol.pdf',
'slug' => 'chromatin-shearing-from-tissue-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-09-29 22:10:32',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
[maximum depth reached]
)
)
),
'Publication' => array(
(int) 0 => array(
'id' => '3630',
'name' => 'Hyper-Editing of Cell-Cycle Regulatory and Tumor Suppressor RNA Promotes Malignant Progenitor Propagation.',
'authors' => 'Jiang Q, Isquith J, Zipeto MA, Diep RH, Pham J, Delos Santos N, Reynoso E, Chau J, Leu H, Lazzari E, Melese E, Ma W, Fang R, Minden M, Morris S, Ren B, Pineda G, Holm F, Jamieson C',
'description' => '<p>Adenosine deaminase associated with RNA1 (ADAR1) deregulation contributes to therapeutic resistance in many malignancies. Here we show that ADAR1-induced hyper-editing in normal human hematopoietic progenitors impairs miR-26a maturation, which represses CDKN1A expression indirectly via EZH2, thereby accelerating cell-cycle transit. However, in blast crisis chronic myeloid leukemia progenitors, loss of EZH2 expression and increased CDKN1A oppose cell-cycle transit. Moreover, A-to-I editing of both the MDM2 regulatory microRNA and its binding site within the 3' UTR region stabilizes MDM2 transcripts, thereby enhancing blast crisis progenitor propagation. These data reveal a dual mechanism governing malignant transformation of progenitors that is predicated on hyper-editing of cell-cycle-regulatory miRNAs and the 3' UTR binding site of tumor suppressor miRNAs.</p>',
'date' => '2019-01-14',
'pmid' => 'http://www.pubmed.gov/30612940',
'doi' => '10.1016/j.ccell.2018.11.017',
'modified' => '2019-05-08 12:25:16',
'created' => '2019-04-25 11:11:44',
'ProductsPublication' => array(
[maximum depth reached]
)
)
),
'Testimonial' => array(),
'Area' => array(),
'SafetySheet' => array(
(int) 0 => array(
'id' => '606',
'name' => 'Elution Module SDS GB en',
'language' => 'en',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-GB-en-1_0.pdf',
'countries' => 'GB',
'modified' => '2020-07-01 15:35:16',
'created' => '2020-07-01 15:35:16',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '608',
'name' => 'Elution Module SDS US en',
'language' => 'en',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-US-en-1_0.pdf',
'countries' => 'US',
'modified' => '2020-07-01 15:36:01',
'created' => '2020-07-01 15:36:01',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '603',
'name' => 'Elution Module SDS DE de',
'language' => 'de',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-DE-de-1_0.pdf',
'countries' => 'DE',
'modified' => '2020-07-01 15:34:04',
'created' => '2020-07-01 15:34:04',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '607',
'name' => 'Elution Module SDS JP ja',
'language' => 'ja',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-JP-ja-1_1.pdf',
'countries' => 'JP',
'modified' => '2020-07-01 15:35:39',
'created' => '2020-07-01 15:35:39',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '602',
'name' => 'Elution Module SDS BE nl',
'language' => 'nl',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-BE-nl-1_0.pdf',
'countries' => 'BE',
'modified' => '2020-07-01 15:33:26',
'created' => '2020-07-01 15:33:26',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '601',
'name' => 'Elution Module SDS BE fr',
'language' => 'fr',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-BE-fr-1_0.pdf',
'countries' => 'BE',
'modified' => '2020-07-01 15:32:52',
'created' => '2020-07-01 15:32:52',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 6 => array(
'id' => '605',
'name' => 'Elution Module SDS FR fr',
'language' => 'fr',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-FR-fr-1_0.pdf',
'countries' => 'FR',
'modified' => '2020-07-01 15:34:55',
'created' => '2020-07-01 15:34:55',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
),
(int) 7 => array(
'id' => '604',
'name' => 'Elution Module SDS ES es',
'language' => 'es',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-ES-es-1_0.pdf',
'countries' => 'ES',
'modified' => '2020-07-01 15:34:32',
'created' => '2020-07-01 15:34:32',
'ProductsSafetySheet' => array(
[maximum depth reached]
)
)
)
)
$country = 'US'
$countries_allowed = array(
(int) 0 => 'CA',
(int) 1 => 'US',
(int) 2 => 'IE',
(int) 3 => 'GB',
(int) 4 => 'DK',
(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = '<br/><small><span style="color:#CCC">(mc-magme-002)</span></small>'
$country_code = 'US'
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$protocol = array(
'id' => '1',
'name' => 'Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor<sup>®</sup> Standard, Plus or Pico',
'description' => '<p>This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chromatin can be used for chromatin immunoprecipitation assays using Diagenode’s iDeal ChIP-seq kit (C01010051). The following protocol is optimized for 30-40 mg of tissue allowing up to 18 ChIP samples (1.5-2 mg of tissue per sample). However, the exact amount of tissue needed for ChIP may vary depending on protein abundance, antibody affinity etc. and should be determined for each tissue type.</p>',
'image_id' => '222',
'type' => 'Protocol',
'url' => 'files/protocols/Chromatin_shearing_from_tissue_protocol.pdf',
'slug' => 'chromatin-shearing-from-tissue-protocol',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-09-29 22:10:32',
'created' => '2015-07-20 10:35:07',
'ProductsProtocol' => array(
'id' => '39',
'product_id' => '1855',
'protocol_id' => '1'
)
)
$application = array(
'id' => '10',
'position' => '10',
'parent_id' => '2',
'name' => 'ChIP-qPCR',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns text-justify">
<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
<li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li>
</ol>
</div>
</div>
<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
<div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div>
</div>
</div>
</div>
</div>',
'in_footer' => false,
'in_menu' => true,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr',
'meta_keywords' => 'Chromatin immunoprecipitation,ChIP Quantitative PCR,polymerase chain reaction (PCR)',
'meta_description' => 'Diagenode's ChIP qPCR kits can be used to quantify enriched DNA after chromatin immunoprecipitation. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of',
'meta_title' => 'ChIP Quantitative PCR (ChIP-qPCR) | Diagenode',
'modified' => '2018-01-09 16:46:56',
'created' => '2014-12-11 00:22:08',
'ProductsApplication' => array(
'id' => '2134',
'product_id' => '1855',
'application_id' => '10'
)
)
$slugs = array(
(int) 0 => 'chip-qpcr'
)
$applications = array(
'id' => '10',
'position' => '10',
'parent_id' => '2',
'name' => 'ChIP-qPCR',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns text-justify">
<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="../categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
<li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li>
</ol>
</div>
</div>
<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
<div class="small-6 medium-6 large-6 columns"><a href="../pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div>
</div>
</div>
</div>
</div>',
'in_footer' => false,
'in_menu' => true,
'online' => true,
'tabular' => true,
'slug' => 'chip-qpcr',
'meta_keywords' => 'Chromatin immunoprecipitation,ChIP Quantitative PCR,polymerase chain reaction (PCR)',
'meta_description' => 'Diagenode's ChIP qPCR kits can be used to quantify enriched DNA after chromatin immunoprecipitation. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of',
'meta_title' => 'ChIP Quantitative PCR (ChIP-qPCR) | Diagenode',
'modified' => '2018-01-09 16:46:56',
'created' => '2014-12-11 00:22:08',
'locale' => 'zho'
)
$description = '<div class="row">
<div class="small-12 medium-12 large-12 columns text-justify">
<p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p>
<p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p>
</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div>
<div class="small-12 medium-12 large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="../categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li>
<li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li>
<li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li>
</ol>
</div>
</div>
<div class="row" style="margin-top: 32px;">
<div class="small-12 medium-10 large-9 small-centered columns">
<div class="radius panel" style="background-color: #fff;">
<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div>
<div class="small-6 medium-6 large-6 columns"><a href="../pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div>
</div>
</div>
</div>
</div>'
$name = 'ChIP-qPCR'
$document = array(
'id' => '68',
'name' => 'DNA Elution Module',
'description' => '<div class="page" title="Page 4">
<div class="section">
<div class="layoutArea">
<div class="column">
<p><span>The DNA elution module allows the elution of chromatin and DNA from immunoprecipitated material after ChIP and MeDIP respectively. Afterwards, you can proceed to the DNA purification using columns or carrying out a phenol/ chloroform/isomayl alcohol extraction. </span></p>
</div>
</div>
</div>
</div>',
'image_id' => null,
'type' => 'Manual',
'url' => 'files/products/kits/Elution_Module_manual.pdf',
'slug' => 'elution-module-manual',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2015-09-01 11:55:17',
'created' => '2015-07-07 11:47:43',
'ProductsDocument' => array(
'id' => '280',
'product_id' => '1855',
'document_id' => '68'
)
)
$sds = array(
'id' => '604',
'name' => 'Elution Module SDS ES es',
'language' => 'es',
'url' => 'files/SDS/Elution/SDS-C01010120-Elution_Module-ES-es-1_0.pdf',
'countries' => 'ES',
'modified' => '2020-07-01 15:34:32',
'created' => '2020-07-01 15:34:32',
'ProductsSafetySheet' => array(
'id' => '1139',
'product_id' => '1855',
'safety_sheet_id' => '604'
)
)
$publication = array(
'id' => '3630',
'name' => 'Hyper-Editing of Cell-Cycle Regulatory and Tumor Suppressor RNA Promotes Malignant Progenitor Propagation.',
'authors' => 'Jiang Q, Isquith J, Zipeto MA, Diep RH, Pham J, Delos Santos N, Reynoso E, Chau J, Leu H, Lazzari E, Melese E, Ma W, Fang R, Minden M, Morris S, Ren B, Pineda G, Holm F, Jamieson C',
'description' => '<p>Adenosine deaminase associated with RNA1 (ADAR1) deregulation contributes to therapeutic resistance in many malignancies. Here we show that ADAR1-induced hyper-editing in normal human hematopoietic progenitors impairs miR-26a maturation, which represses CDKN1A expression indirectly via EZH2, thereby accelerating cell-cycle transit. However, in blast crisis chronic myeloid leukemia progenitors, loss of EZH2 expression and increased CDKN1A oppose cell-cycle transit. Moreover, A-to-I editing of both the MDM2 regulatory microRNA and its binding site within the 3' UTR region stabilizes MDM2 transcripts, thereby enhancing blast crisis progenitor propagation. These data reveal a dual mechanism governing malignant transformation of progenitors that is predicated on hyper-editing of cell-cycle-regulatory miRNAs and the 3' UTR binding site of tumor suppressor miRNAs.</p>',
'date' => '2019-01-14',
'pmid' => 'http://www.pubmed.gov/30612940',
'doi' => '10.1016/j.ccell.2018.11.017',
'modified' => '2019-05-08 12:25:16',
'created' => '2019-04-25 11:11:44',
'ProductsPublication' => array(
'id' => '3574',
'product_id' => '1855',
'publication_id' => '3630'
)
)
$externalLink = ' <a href="http://www.pubmed.gov/30612940" target="_blank"><i class="fa fa-external-link"></i></a>'
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
×