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FOXA2 polyclonal antibody

Histone-Deacetylase-polyclonal-antibody-diagenode

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

Antibody portal

Antibodies

Catalog Number

Format

Price

C15410343

50 μg

$380.00
Bulk order

Other format

C15410343-10

10 µg

Other names: HNF3B, TFC3B, HNF-3B, TCF-3B

Polyclonal antibody raised in rabbit against human FOXA2 (Forkhead Box A2), using two synthetic peptides containing a sequence from the central part and the N-terminus of the protein, respectively.

Lot	A2683-0040
Concentration	2.4 μg/μl
Species reactivity	Human: positive. Other species: not tested.
Type	Polyclonal
Purity	Affinity purified polyclonal antibody in PBS containing 0.05% azide.
Host	Rabbit
Storage Conditions	Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ChIP/ChIP-seq ^*	2 μg/ChIP	Fig 1, 2
ELISA	1:5,000	Fig 3
WB	1:500	Fig 4

^* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

Validation data

Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA2
ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA2 (cat. No. C15410343) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the AFP and TTR genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA2
ChIP was performed with 2 μg of the Diagenode antibody against FOXA2 (Cat. No. C15410343) on sheared chromatin from 4,000,000 HepG2 cells using the “iDeal ChIP-seq” kit as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 1.5 Mb region of the human X-chromosome (figures 2A and B), and in two genomic regions surrounding the AFP and TTR positive control genes (figure 2C and D).

Figure 3. Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against FOXA2 (Cat. No. C15410343). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.

Figure 4. Western blot analysis using the Diagenode antibody directed against FOXA2
Whole cell extracts from HepG2 cells were analysed by Western blot using the Diagenode antibody against FOXA2 (Cat. No. C15410343) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Target Description
FOXA2 (UniProtKB/Swiss-Prot entry Q9Y261) is a member of the forkhead class of transcription factors which are involved in the transcriptional activation of liver-specific genes such as AFP, albumin, tyrosine aminotransferase, PEPCK and transthyretin. FOXA2 also plays a role glucose homeostasis and in the regulation of fat metabolism and is required for the embryonic development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs. The FOXA2 gene has been linked to sporadic cases of maturity-onset diabetes of the young.

資料
FOXA2 polyclonal antibody DATASHEET
Polyclonal antibody raised in rabbit against human FOXA2 (Forkhead Box A2), using two synthetic p... Download

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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA2 (cat. No. C15410343) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the AFP and TTR genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP was performed with 2 &mu;g of the Diagenode antibody against FOXA2 (Cat. No. C15410343) on sheared chromatin from 4,000,000 HepG2 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 1.5 Mb region of the human X-chromosome (figures 2A and B), and in two genomic regions surrounding the AFP and TTR positive control genes (figure 2C and D).</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against FOXA2 (Cat. No. C15410343). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-wb.jpg" alt="FOXA2 Antibody validated in Western Blot" caption="false" width="250" height="329" /></p>
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<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against FOXA2</strong><br />Whole cell extracts from HepG2 cells were analysed by Western blot using the Diagenode antibody against FOXA2 (Cat. No. C15410343) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<td>Fig 4</td>
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA2 (cat. No. C15410343) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the AFP and TTR genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against FOXA2 (Cat. No. C15410343). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-wb.jpg" alt="FOXA2 Antibody validated in Western Blot" caption="false" width="250" height="329" /></p>
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<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against FOXA2</strong><br />Whole cell extracts from HepG2 cells were analysed by Western blot using the Diagenode antibody against FOXA2 (Cat. No. C15410343) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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FOXA2 also plays a role glucose homeostasis and in the regulation of fat metabolism and is required for the embryonic
development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs. The FOXA2 gene has been
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<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 &mu;g/ChIP</td>
<td>Fig 1, 2</td>
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<td>ELISA</td>
<td>1:5,000</td>
<td>Fig 3</td>
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<p>Polyclonal antibody raised in rabbit against human <strong>FOXA2 (Forkhead Box A2)</strong>, using two synthetic peptides containing a sequence from the central part and the N-terminus of the protein, respectively.</p>',
			'label1' => 'Validation data',
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<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chip.jpg" alt="FOXA2 Antibody ChIP Grade" caption="false" width="432" height="328" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA2 (cat. No. C15410343) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the AFP and TTR genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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</div>
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<div class="small-12 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-a.jpg" alt="FOXA2 Antibody ChIP-seq Grade" caption="false" width="700" height="83" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-b.jpg" alt="FOXA2 Antibody for ChIP-seq " caption="false" width="700" height="224" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-c.jpg" alt="FOXA2 Antibody for ChIP-seq assay" caption="false" width="700" height="104" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-d.jpg" alt="FOXA2 Antibody validated in ChIP-seq" caption="false" width="700" height="102" /></p>
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<div class="small-12 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP was performed with 2 &mu;g of the Diagenode antibody against FOXA2 (Cat. No. C15410343) on sheared chromatin from 4,000,000 HepG2 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 1.5 Mb region of the human X-chromosome (figures 2A and B), and in two genomic regions surrounding the AFP and TTR positive control genes (figure 2C and D).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-elisa.jpg" alt="FOXA2 Antibody ELISA Validation" caption="false" width="432" height="328" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against FOXA2 (Cat. No. C15410343). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-wb.jpg" alt="FOXA2 Antibody validated in Western Blot" caption="false" width="250" height="329" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against FOXA2</strong><br />Whole cell extracts from HepG2 cells were analysed by Western blot using the Diagenode antibody against FOXA2 (Cat. No. C15410343) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<div class="small-10 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
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<p></p>
<p></p>
<p></p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Expert technical support</span></li>
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<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 100% satisfaction guarantee</span></li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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			'description' => '<p><strong>Other names:</strong> HNF3B, TFC3B, HNF-3B, TCF-3B</p><p>Polyclonal antibody raised in rabbit against human FOXA2 (Forkhead Box A2), using two synthetic peptides containing a sequence from the central part and the N-terminus of the protein, respectively.</p>',
			'label1' => 'Validation data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chip.jpg" alt="FOXA2 Antibody ChIP Grade" caption="false" width="432" height="328" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA2 (cat. No. C15410343) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the AFP and TTR genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<div class="row">
<div class="small-12 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-a.jpg" alt="FOXA2 Antibody ChIP-seq Grade" caption="false" width="700" height="83" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-b.jpg" alt="FOXA2 Antibody for ChIP-seq " caption="false" width="700" height="224" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-c.jpg" alt="FOXA2 Antibody for ChIP-seq assay" caption="false" width="700" height="104" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-d.jpg" alt="FOXA2 Antibody validated in ChIP-seq" caption="false" width="700" height="102" /></p>
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<div class="row">
<div class="small-12 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP was performed with 2 &mu;g of the Diagenode antibody against FOXA2 (Cat. No. C15410343) on sheared chromatin from 4,000,000 HepG2 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 1.5 Mb region of the human X-chromosome (figures 2A and B), and in two genomic regions surrounding the AFP and TTR positive control genes (figure 2C and D).</small></p>
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<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-elisa.jpg" alt="FOXA2 Antibody ELISA Validation" caption="false" width="432" height="328" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against FOXA2 (Cat. No. C15410343). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-wb.jpg" alt="FOXA2 Antibody validated in Western Blot" caption="false" width="250" height="329" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against FOXA2</strong><br />Whole cell extracts from HepG2 cells were analysed by Western blot using the Diagenode antibody against FOXA2 (Cat. No. C15410343) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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FOXA2 also plays a role glucose homeostasis and in the regulation of fat metabolism and is required for the embryonic
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<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
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<tbody>
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<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 &mu;g/ChIP</td>
<td>Fig 1, 2</td>
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<tr>
<td>ELISA</td>
<td>1:5,000</td>
<td>Fig 3</td>
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<td>WB</td>
<td>1:500</td>
<td>Fig 4</td>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 &mu;g per IP.</small></p>',
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			'modified' => '2019-09-10 14:41:10',
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		'antibody_id' => '517',
		'name' => 'FOXA2 polyclonal antibody',
		'description' => '<p><strong>Other names:</strong> HNF3B, TFC3B, HNF-3B, TCF-3B</p><p>Polyclonal antibody raised in rabbit against human FOXA2 (Forkhead Box A2), using two synthetic peptides containing a sequence from the central part and the N-terminus of the protein, respectively.</p>',
		'label1' => 'Validation data',
		'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chip.jpg" alt="FOXA2 Antibody ChIP Grade" caption="false" width="432" height="328" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA2 (cat. No. C15410343) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the AFP and TTR genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<div class="small-12 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-a.jpg" alt="FOXA2 Antibody ChIP-seq Grade" caption="false" width="700" height="83" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-b.jpg" alt="FOXA2 Antibody for ChIP-seq " caption="false" width="700" height="224" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-c.jpg" alt="FOXA2 Antibody for ChIP-seq assay" caption="false" width="700" height="104" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-d.jpg" alt="FOXA2 Antibody validated in ChIP-seq" caption="false" width="700" height="102" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP was performed with 2 &mu;g of the Diagenode antibody against FOXA2 (Cat. No. C15410343) on sheared chromatin from 4,000,000 HepG2 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 1.5 Mb region of the human X-chromosome (figures 2A and B), and in two genomic regions surrounding the AFP and TTR positive control genes (figure 2C and D).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-elisa.jpg" alt="FOXA2 Antibody ELISA Validation" caption="false" width="432" height="328" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against FOXA2 (Cat. No. C15410343). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
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</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-wb.jpg" alt="FOXA2 Antibody validated in Western Blot" caption="false" width="250" height="329" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against FOXA2</strong><br />Whole cell extracts from HepG2 cells were analysed by Western blot using the Diagenode antibody against FOXA2 (Cat. No. C15410343) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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		'host' => '*****',
		'id' => '517',
		'name' => 'FOXA2 polyclonal antibody',
		'description' => 'FOXA2 (UniProtKB/Swiss-Prot entry Q9Y261) is a member of the forkhead class of transcription factors which are involved in the
transcriptional activation of liver-specific genes such as AFP, albumin, tyrosine aminotransferase, PEPCK and transthyretin.
FOXA2 also plays a role glucose homeostasis and in the regulation of fat metabolism and is required for the embryonic
development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs. The FOXA2 gene has been
linked to sporadic cases of maturity-onset diabetes of the young.',
		'clonality' => '',
		'isotype' => '',
		'lot' => 'A2683-0040',
		'concentration' => '2.4 μg/μl',
		'reactivity' => 'Human: positive. Other species: not tested.',
		'type' => 'Polyclonal',
		'purity' => 'Affinity purified polyclonal antibody in PBS containing 0.05% azide.',
		'classification' => '',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 &mu;g/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:5,000</td>
<td>Fig 3</td>
</tr>
<tr>
<td>WB</td>
<td>1:500</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 &mu;g per IP.</small></p>',
		'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
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			'name' => 'FOXA2 Antibody',
			'description' => '<p><strong>Other names:</strong> HNF3B, TFC3B, HNF-3B, TCF-3B</p>
<p>Polyclonal antibody raised in rabbit against human <strong>FOXA2 (Forkhead Box A2)</strong>, using two synthetic peptides containing a sequence from the central part and the N-terminus of the protein, respectively.</p>',
			'label1' => 'Validation data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chip.jpg" alt="FOXA2 Antibody ChIP Grade" caption="false" width="432" height="328" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA2 (cat. No. C15410343) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the AFP and TTR genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-a.jpg" alt="FOXA2 Antibody ChIP-seq Grade" caption="false" width="700" height="83" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-b.jpg" alt="FOXA2 Antibody for ChIP-seq " caption="false" width="700" height="224" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-c.jpg" alt="FOXA2 Antibody for ChIP-seq assay" caption="false" width="700" height="104" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-d.jpg" alt="FOXA2 Antibody validated in ChIP-seq" caption="false" width="700" height="102" /></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP was performed with 2 &mu;g of the Diagenode antibody against FOXA2 (Cat. No. C15410343) on sheared chromatin from 4,000,000 HepG2 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 1.5 Mb region of the human X-chromosome (figures 2A and B), and in two genomic regions surrounding the AFP and TTR positive control genes (figure 2C and D).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-elisa.jpg" alt="FOXA2 Antibody ELISA Validation" caption="false" width="432" height="328" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against FOXA2 (Cat. No. C15410343). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-wb.jpg" alt="FOXA2 Antibody validated in Western Blot" caption="false" width="250" height="329" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against FOXA2</strong><br />Whole cell extracts from HepG2 cells were analysed by Western blot using the Diagenode antibody against FOXA2 (Cat. No. C15410343) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>',
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			'meta_description' => 'FOXA2 (Forkhead Box A2) Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: HNF3B, TFC3B, HNF-3B, TCF-3B. Sample size available.',
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			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<div class="small-10 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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			'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP',
			'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode',
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			'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
</div>
<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
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			'in_menu' => true,
			'online' => true,
			'tabular' => false,
			'hide' => true,
			'all_format' => false,
			'is_antibody' => true,
			'slug' => 'chip-seq-grade-antibodies',
			'cookies_tag_id' => null,
			'meta_keywords' => 'ChIP-seq grade antibodies,polyclonal antibody,WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab)',
			'meta_description' => 'Diagenode Offers Wide Range of Validated ChIP-Seq Grade Antibodies for Unparalleled ChIP-Seq Results',
			'meta_title' => 'Chromatin Immunoprecipitation ChIP-Seq Grade Antibodies | Diagenode',
			'modified' => '2019-07-03 10:57:22',
			'created' => '2015-02-16 02:24:01',
			'ProductsCategory' => array(
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		),
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			'id' => '30',
			'position' => '50',
			'parent_id' => '4',
			'name' => 'Transcription',
			'description' => '<p><span style="font-weight: 400;">The list of Diagenode&rsquo;s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. &nbsp;Check the list below to see our targets.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Expert technical support</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Sample sizes available</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 100% satisfaction guarantee</span></li>
</ul>',
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			'tabular' => false,
			'hide' => true,
			'all_format' => false,
			'is_antibody' => true,
			'slug' => 'transcription-factor',
			'cookies_tag_id' => null,
			'meta_keywords' => ' Transcription factor antibodies,monoclonal antibodies,polyclonal antibodies',
			'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie',
			'meta_title' => 'Transcription factor Antibodies | Diagenode',
			'modified' => '2020-07-06 12:59:19',
			'created' => '2015-03-12 10:20:08',
			'ProductsCategory' => array(
				[maximum depth reached]
			),
			'CookiesTag' => array([maximum depth reached])
		),
		(int) 3 => array(
			'id' => '103',
			'position' => '0',
			'parent_id' => '4',
			'name' => 'All antibodies',
			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
</ul>',
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			'in_menu' => true,
			'online' => true,
			'tabular' => false,
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			'all_format' => false,
			'is_antibody' => true,
			'slug' => 'all-antibodies',
			'cookies_tag_id' => null,
			'meta_keywords' => 'Antibodies,Premium Antibodies,Classic,Pioneer',
			'meta_description' => 'Diagenode Offers Strict quality standards with Rigorous QC and validated Antibodies. Classified based on level of validation for flexibility of Application. Comprehensive selection of histone and non-histone Antibodies',
			'meta_title' => 'Diagenode's selection of Antibodies is exclusively dedicated for Epigenetic Research | Diagenode',
			'modified' => '2019-07-03 10:55:44',
			'created' => '2015-11-02 14:49:22',
			'ProductsCategory' => array(
				[maximum depth reached]
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			'CookiesTag' => array([maximum depth reached])
		)
	),
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		(int) 0 => array(
			'id' => '979',
			'name' => 'FOXA2 polyclonal antibody',
			'description' => '<p>Polyclonal antibody raised in rabbit against human FOXA2 (Forkhead Box A2), using two synthetic peptides containing a sequence from the central part and the N-terminus of the protein, respectively.</p>',
			'image_id' => null,
			'type' => 'Datasheet',
			'url' => 'files/products/antibodies/Datasheet_FOXA2_C15410343.pdf',
			'slug' => 'foxa2-polyclonal-antibody',
			'meta_keywords' => '',
			'meta_description' => '',
			'modified' => '2018-01-03 11:11:03',
			'created' => '2018-01-03 11:11:03',
			'ProductsDocument' => array(
				[maximum depth reached]
			)
		)
	),
	'Feature' => array(),
	'Image' => array(
		(int) 0 => array(
			'id' => '251',
			'name' => 'product/antibodies/chipseq-abs.png',
			'alt' => 'Histone-Deacetylase-polyclonal-antibody-diagenode',
			'modified' => '2018-04-17 12:12:14',
			'created' => '2015-07-17 10:12:18',
			'ProductsImage' => array(
				[maximum depth reached]
			)
		)
	),
	'Promotion' => array(),
	'Protocol' => array(),
	'Publication' => array(),
	'Testimonial' => array(),
	'Area' => array(),
	'SafetySheet' => array(
		(int) 0 => array(
			'id' => '3781',
			'name' => 'SDS C15410343 FOXA2 Antibody US en',
			'language' => 'en',
			'url' => 'files/SDS/FOXA2/SDS-C15410343-FOXA2_Antibody-US-en-GHS_2_0.pdf',
			'countries' => 'US',
			'modified' => '2024-01-17 19:19:24',
			'created' => '2024-01-17 19:19:24',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 1 => array(
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			'name' => 'SDS C15410343 FOXA2 Antibody GB en',
			'language' => 'en',
			'url' => 'files/SDS/FOXA2/SDS-C15410343-FOXA2_Antibody-GB-en-GHS_2_0.pdf',
			'countries' => 'GB',
			'modified' => '2024-01-17 19:20:02',
			'created' => '2024-01-17 19:20:02',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 2 => array(
			'id' => '3784',
			'name' => 'SDS C15410343 FOXA2 Antibody FR fr',
			'language' => 'fr',
			'url' => 'files/SDS/FOXA2/SDS-C15410343-FOXA2_Antibody-FR-fr-GHS_2_0.pdf',
			'countries' => 'FR',
			'modified' => '2024-01-17 19:20:22',
			'created' => '2024-01-17 19:20:22',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 3 => array(
			'id' => '3780',
			'name' => 'SDS C15410343 FOXA2 Antibody BE fr',
			'language' => 'fr',
			'url' => 'files/SDS/FOXA2/SDS-C15410343-FOXA2_Antibody-BE-fr-GHS_2_0.pdf',
			'countries' => 'BE',
			'modified' => '2024-01-17 19:19:05',
			'created' => '2024-01-17 19:19:05',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 4 => array(
			'id' => '3779',
			'name' => 'SDS C15410343 FOXA2 Antibody BE nl',
			'language' => 'nl',
			'url' => 'files/SDS/FOXA2/SDS-C15410343-FOXA2_Antibody-BE-nl-GHS_2_0.pdf',
			'countries' => 'BE',
			'modified' => '2024-01-17 19:18:51',
			'created' => '2024-01-17 19:18:51',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 5 => array(
			'id' => '3778',
			'name' => 'SDS C15410343 FOXA2 Antibody DE de',
			'language' => 'de',
			'url' => 'files/SDS/FOXA2/SDS-C15410343-FOXA2_Antibody-DE-de-GHS_2_0.pdf',
			'countries' => 'DE',
			'modified' => '2024-01-17 19:18:32',
			'created' => '2024-01-17 19:18:32',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 6 => array(
			'id' => '3782',
			'name' => 'SDS C15410343 FOXA2 Antibody JP ja',
			'language' => 'ja',
			'url' => 'files/SDS/FOXA2/SDS-C15410343-FOXA2_Antibody-JP-ja-GHS_2_0.pdf',
			'countries' => 'JP',
			'modified' => '2024-01-17 19:19:43',
			'created' => '2024-01-17 19:19:43',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		),
		(int) 7 => array(
			'id' => '3785',
			'name' => 'SDS C15410343 FOXA2 Antibody ES es',
			'language' => 'es',
			'url' => 'files/SDS/FOXA2/SDS-C15410343-FOXA2_Antibody-ES-es-GHS_2_0.pdf',
			'countries' => 'ES',
			'modified' => '2024-01-17 19:20:39',
			'created' => '2024-01-17 19:20:39',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
		)
	)
)
$meta_canonical = 'https://www.diagenode.com/jp/p/foxa2-polyclonal-antibody-50-ug'
$country = 'US'
$countries_allowed = array(
	(int) 0 => 'CA',
	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
	(int) 0 => array(
		'id' => '2929',
		'antibody_id' => '517',
		'name' => 'FOXA2 Antibody (sample size)',
		'description' => '',
		'label1' => 'Validation data',
		'info1' => '',
		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
		'format' => '10 µg',
		'catalog_number' => 'C15410343-10',
		'old_catalog_number' => '',
		'sf_code' => 'C15410343-D001-000582',
		'type' => 'FRE',
		'search_order' => '03-Antibody',
		'price_EUR' => '105',
		'price_USD' => '115',
		'price_GBP' => '100',
		'price_JPY' => '16450',
		'price_CNY' => '0',
		'price_AUD' => '288',
		'country' => 'ALL',
		'except_countries' => 'None',
		'quote' => false,
		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => false,
		'last_datasheet_update' => 'November 21, 2017',
		'slug' => 'foxa2-polyclonal-antibody-10-ug',
		'meta_title' => 'FOXA2 Antibody  - ChIP-seq Grade (C15410343) | Diagenode',
		'meta_keywords' => '',
		'meta_description' => 'FOXA2 (Forkhead Box A2) Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, ELISA and WB. Batch-specific data available on the website. Other names: HNF3B, TFC3B, HNF-3B, TCF-3B. Sample size available.',
		'modified' => '2024-01-17 19:21:32',
		'created' => '2018-01-03 10:37:02',
		'ProductsGroup' => array(
			'id' => '256',
			'product_id' => '2929',
			'group_id' => '231'
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	)
)
$pro = array(
	'id' => '2929',
	'antibody_id' => '517',
	'name' => 'FOXA2 Antibody (sample size)',
	'description' => '',
	'label1' => 'Validation data',
	'info1' => '',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '10 µg',
	'catalog_number' => 'C15410343-10',
	'old_catalog_number' => '',
	'sf_code' => 'C15410343-D001-000582',
	'type' => 'FRE',
	'search_order' => '03-Antibody',
	'price_EUR' => '105',
	'price_USD' => '115',
	'price_GBP' => '100',
	'price_JPY' => '16450',
	'price_CNY' => '0',
	'price_AUD' => '288',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => false,
	'last_datasheet_update' => 'November 21, 2017',
	'slug' => 'foxa2-polyclonal-antibody-10-ug',
	'meta_title' => 'FOXA2 Antibody  - ChIP-seq Grade (C15410343) | Diagenode',
	'meta_keywords' => '',
	'meta_description' => 'FOXA2 (Forkhead Box A2) Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, ELISA and WB. Batch-specific data available on the website. Other names: HNF3B, TFC3B, HNF-3B, TCF-3B. Sample size available.',
	'modified' => '2024-01-17 19:21:32',
	'created' => '2018-01-03 10:37:02',
	'ProductsGroup' => array(
		'id' => '256',
		'product_id' => '2929',
		'group_id' => '231'
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)
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = ''
$rrbs_service = array(
	(int) 0 => (int) 1894,
	(int) 1 => (int) 1895
)
$chipseq_service = array(
	(int) 0 => (int) 2683,
	(int) 1 => (int) 1835,
	(int) 2 => (int) 1836,
	(int) 3 => (int) 2684,
	(int) 4 => (int) 1838,
	(int) 5 => (int) 1839,
	(int) 6 => (int) 1856
)
$labelize = object(Closure) {
	
}
$old_catalog_number = ''
$country_code = 'US'
$other_format = array(
	'id' => '2929',
	'antibody_id' => '517',
	'name' => 'FOXA2 Antibody (sample size)',
	'description' => '',
	'label1' => 'Validation data',
	'info1' => '',
	'label2' => '',
	'info2' => '',
	'label3' => '',
	'info3' => '',
	'format' => '10 µg',
	'catalog_number' => 'C15410343-10',
	'old_catalog_number' => '',
	'sf_code' => 'C15410343-D001-000582',
	'type' => 'FRE',
	'search_order' => '03-Antibody',
	'price_EUR' => '105',
	'price_USD' => '115',
	'price_GBP' => '100',
	'price_JPY' => '16450',
	'price_CNY' => '0',
	'price_AUD' => '288',
	'country' => 'ALL',
	'except_countries' => 'None',
	'quote' => false,
	'in_stock' => false,
	'featured' => false,
	'no_promo' => false,
	'online' => true,
	'master' => false,
	'last_datasheet_update' => 'November 21, 2017',
	'slug' => 'foxa2-polyclonal-antibody-10-ug',
	'meta_title' => 'FOXA2 Antibody  - ChIP-seq Grade (C15410343) | Diagenode',
	'meta_keywords' => '',
	'meta_description' => 'FOXA2 (Forkhead Box A2) Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, ELISA and WB. Batch-specific data available on the website. Other names: HNF3B, TFC3B, HNF-3B, TCF-3B. Sample size available.',
	'modified' => '2024-01-17 19:21:32',
	'created' => '2018-01-03 10:37:02',
	'ProductsGroup' => array(
		'id' => '256',
		'product_id' => '2929',
		'group_id' => '231'
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)
$img = 'banners/banner-cut_tag-chipmentation-500.jpg'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$application = array(
	'id' => '19',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'western-blot-antibodies',
	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
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	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
	'modified' => '2016-04-26 12:44:51',
	'created' => '2015-01-07 09:20:00',
	'ProductsApplication' => array(
		'id' => '4760',
		'product_id' => '2922',
		'application_id' => '19'
	)
)
$slugs = array(
	(int) 0 => 'western-blot-antibodies'
)
$applications = array(
	'id' => '19',
	'position' => '10',
	'parent_id' => '40',
	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
	'in_footer' => false,
	'in_menu' => false,
	'online' => true,
	'tabular' => true,
	'slug' => 'western-blot-antibodies',
	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
	'meta_description' => 'Diagenode offers a wide range of antibodies and technical support for western blot applications',
	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
	'modified' => '2016-04-26 12:44:51',
	'created' => '2015-01-07 09:20:00',
	'locale' => 'jpn'
)
$description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
$name = 'WB'
$document = array(
	'id' => '979',
	'name' => 'FOXA2 polyclonal antibody',
	'description' => '<p>Polyclonal antibody raised in rabbit against human FOXA2 (Forkhead Box A2), using two synthetic peptides containing a sequence from the central part and the N-terminus of the protein, respectively.</p>',
	'image_id' => null,
	'type' => 'Datasheet',
	'url' => 'files/products/antibodies/Datasheet_FOXA2_C15410343.pdf',
	'slug' => 'foxa2-polyclonal-antibody',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2018-01-03 11:11:03',
	'created' => '2018-01-03 11:11:03',
	'ProductsDocument' => array(
		'id' => '2482',
		'product_id' => '2922',
		'document_id' => '979'
	)
)
$sds = array(
	'id' => '3785',
	'name' => 'SDS C15410343 FOXA2 Antibody ES es',
	'language' => 'es',
	'url' => 'files/SDS/FOXA2/SDS-C15410343-FOXA2_Antibody-ES-es-GHS_2_0.pdf',
	'countries' => 'ES',
	'modified' => '2024-01-17 19:20:39',
	'created' => '2024-01-17 19:20:39',
	'ProductsSafetySheet' => array(
		'id' => '6189',
		'product_id' => '2922',
		'safety_sheet_id' => '3785'
	)
)
include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
    <?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp'
$dataForView = array(
	'language' => 'jp',
	'meta_keywords' => '',
	'meta_description' => '',
	'meta_title' => 'FOXA2 polyclonal antibody',
	'product' => array(
		'Product' => array(
			'id' => '2922',
			'antibody_id' => '517',
			'name' => 'FOXA2 polyclonal antibody',
			'description' => '<p><strong>Other names:</strong> HNF3B, TFC3B, HNF-3B, TCF-3B</p><p>Polyclonal antibody raised in rabbit against human FOXA2 (Forkhead Box A2), using two synthetic peptides containing a sequence from the central part and the N-terminus of the protein, respectively.</p>',
			'label1' => 'Validation data',
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<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA2 (cat. No. C15410343) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the AFP and TTR genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-a.jpg" alt="FOXA2 Antibody ChIP-seq Grade" caption="false" width="700" height="83" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-b.jpg" alt="FOXA2 Antibody for ChIP-seq " caption="false" width="700" height="224" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-c.jpg" alt="FOXA2 Antibody for ChIP-seq assay" caption="false" width="700" height="104" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-d.jpg" alt="FOXA2 Antibody validated in ChIP-seq" caption="false" width="700" height="102" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP was performed with 2 &mu;g of the Diagenode antibody against FOXA2 (Cat. No. C15410343) on sheared chromatin from 4,000,000 HepG2 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 1.5 Mb region of the human X-chromosome (figures 2A and B), and in two genomic regions surrounding the AFP and TTR positive control genes (figure 2C and D).</small></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against FOXA2 (Cat. No. C15410343). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-wb.jpg" alt="FOXA2 Antibody validated in Western Blot" caption="false" width="250" height="329" /></p>
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<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against FOXA2</strong><br />Whole cell extracts from HepG2 cells were analysed by Western blot using the Diagenode antibody against FOXA2 (Cat. No. C15410343) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 &mu;g/ChIP</td>
<td>Fig 1, 2</td>
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<td>1:5,000</td>
<td>Fig 3</td>
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<td>Fig 4</td>
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		'description' => '<p><strong>Other names:</strong> HNF3B, TFC3B, HNF-3B, TCF-3B</p><p>Polyclonal antibody raised in rabbit against human FOXA2 (Forkhead Box A2), using two synthetic peptides containing a sequence from the central part and the N-terminus of the protein, respectively.</p>',
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<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chip.jpg" alt="FOXA2 Antibody ChIP Grade" caption="false" width="432" height="328" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA2 (cat. No. C15410343) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the AFP and TTR genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-b.jpg" alt="FOXA2 Antibody for ChIP-seq " caption="false" width="700" height="224" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-c.jpg" alt="FOXA2 Antibody for ChIP-seq assay" caption="false" width="700" height="104" /></p>
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<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP was performed with 2 &mu;g of the Diagenode antibody against FOXA2 (Cat. No. C15410343) on sheared chromatin from 4,000,000 HepG2 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 1.5 Mb region of the human X-chromosome (figures 2A and B), and in two genomic regions surrounding the AFP and TTR positive control genes (figure 2C and D).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-elisa.jpg" alt="FOXA2 Antibody ELISA Validation" caption="false" width="432" height="328" /></p>
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<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against FOXA2 (Cat. No. C15410343). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
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<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against FOXA2</strong><br />Whole cell extracts from HepG2 cells were analysed by Western blot using the Diagenode antibody against FOXA2 (Cat. No. C15410343) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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FOXA2 also plays a role glucose homeostasis and in the regulation of fat metabolism and is required for the embryonic
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<th>References</th>
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<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 &mu;g/ChIP</td>
<td>Fig 1, 2</td>
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<tr>
<td>ELISA</td>
<td>1:5,000</td>
<td>Fig 3</td>
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<td>WB</td>
<td>1:500</td>
<td>Fig 4</td>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 &mu;g per IP.</small></p>',
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			'name' => 'FOXA2 Antibody',
			'description' => '<p><strong>Other names:</strong> HNF3B, TFC3B, HNF-3B, TCF-3B</p>
<p>Polyclonal antibody raised in rabbit against human <strong>FOXA2 (Forkhead Box A2)</strong>, using two synthetic peptides containing a sequence from the central part and the N-terminus of the protein, respectively.</p>',
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<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chip.jpg" alt="FOXA2 Antibody ChIP Grade" caption="false" width="432" height="328" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA2 (cat. No. C15410343) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the AFP and TTR genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<div class="row">
<div class="small-12 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-a.jpg" alt="FOXA2 Antibody ChIP-seq Grade" caption="false" width="700" height="83" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-b.jpg" alt="FOXA2 Antibody for ChIP-seq " caption="false" width="700" height="224" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-c.jpg" alt="FOXA2 Antibody for ChIP-seq assay" caption="false" width="700" height="104" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-d.jpg" alt="FOXA2 Antibody validated in ChIP-seq" caption="false" width="700" height="102" /></p>
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<div class="row">
<div class="small-12 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP was performed with 2 &mu;g of the Diagenode antibody against FOXA2 (Cat. No. C15410343) on sheared chromatin from 4,000,000 HepG2 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 1.5 Mb region of the human X-chromosome (figures 2A and B), and in two genomic regions surrounding the AFP and TTR positive control genes (figure 2C and D).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-elisa.jpg" alt="FOXA2 Antibody ELISA Validation" caption="false" width="432" height="328" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against FOXA2 (Cat. No. C15410343). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-wb.jpg" alt="FOXA2 Antibody validated in Western Blot" caption="false" width="250" height="329" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against FOXA2</strong><br />Whole cell extracts from HepG2 cells were analysed by Western blot using the Diagenode antibody against FOXA2 (Cat. No. C15410343) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<div class="small-10 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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			'name' => 'FOXA2 polyclonal antibody',
			'description' => '<p><strong>Other names:</strong> HNF3B, TFC3B, HNF-3B, TCF-3B</p><p>Polyclonal antibody raised in rabbit against human FOXA2 (Forkhead Box A2), using two synthetic peptides containing a sequence from the central part and the N-terminus of the protein, respectively.</p>',
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<div class="small-6 columns">
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<div class="small-6 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA2 (cat. No. C15410343) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the AFP and TTR genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-b.jpg" alt="FOXA2 Antibody for ChIP-seq " caption="false" width="700" height="224" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-c.jpg" alt="FOXA2 Antibody for ChIP-seq assay" caption="false" width="700" height="104" /></p>
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<div class="small-12 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP was performed with 2 &mu;g of the Diagenode antibody against FOXA2 (Cat. No. C15410343) on sheared chromatin from 4,000,000 HepG2 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 1.5 Mb region of the human X-chromosome (figures 2A and B), and in two genomic regions surrounding the AFP and TTR positive control genes (figure 2C and D).</small></p>
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<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-elisa.jpg" alt="FOXA2 Antibody ELISA Validation" caption="false" width="432" height="328" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against FOXA2 (Cat. No. C15410343). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
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<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-wb.jpg" alt="FOXA2 Antibody validated in Western Blot" caption="false" width="250" height="329" /></p>
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<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against FOXA2</strong><br />Whole cell extracts from HepG2 cells were analysed by Western blot using the Diagenode antibody against FOXA2 (Cat. No. C15410343) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
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			'name' => 'FOXA2 polyclonal antibody',
			'description' => 'FOXA2 (UniProtKB/Swiss-Prot entry Q9Y261) is a member of the forkhead class of transcription factors which are involved in the
transcriptional activation of liver-specific genes such as AFP, albumin, tyrosine aminotransferase, PEPCK and transthyretin.
FOXA2 also plays a role glucose homeostasis and in the regulation of fat metabolism and is required for the embryonic
development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs. The FOXA2 gene has been
linked to sporadic cases of maturity-onset diabetes of the young.',
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			'isotype' => '',
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			'concentration' => '2.4 μg/μl',
			'reactivity' => 'Human: positive. Other species: not tested.',
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			'purity' => 'Affinity purified polyclonal antibody in PBS containing 0.05% azide.',
			'classification' => '',
			'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 &mu;g/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:5,000</td>
<td>Fig 3</td>
</tr>
<tr>
<td>WB</td>
<td>1:500</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 &mu;g per IP.</small></p>',
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		'antibody_id' => '517',
		'name' => 'FOXA2 polyclonal antibody',
		'description' => '<p><strong>Other names:</strong> HNF3B, TFC3B, HNF-3B, TCF-3B</p><p>Polyclonal antibody raised in rabbit against human FOXA2 (Forkhead Box A2), using two synthetic peptides containing a sequence from the central part and the N-terminus of the protein, respectively.</p>',
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<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chip.jpg" alt="FOXA2 Antibody ChIP Grade" caption="false" width="432" height="328" /></p>
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<div class="small-6 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA2 (cat. No. C15410343) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the AFP and TTR genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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</div>
<div class="row">
<div class="small-12 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-a.jpg" alt="FOXA2 Antibody ChIP-seq Grade" caption="false" width="700" height="83" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-b.jpg" alt="FOXA2 Antibody for ChIP-seq " caption="false" width="700" height="224" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-c.jpg" alt="FOXA2 Antibody for ChIP-seq assay" caption="false" width="700" height="104" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-d.jpg" alt="FOXA2 Antibody validated in ChIP-seq" caption="false" width="700" height="102" /></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP was performed with 2 &mu;g of the Diagenode antibody against FOXA2 (Cat. No. C15410343) on sheared chromatin from 4,000,000 HepG2 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 1.5 Mb region of the human X-chromosome (figures 2A and B), and in two genomic regions surrounding the AFP and TTR positive control genes (figure 2C and D).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-elisa.jpg" alt="FOXA2 Antibody ELISA Validation" caption="false" width="432" height="328" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against FOXA2 (Cat. No. C15410343). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-wb.jpg" alt="FOXA2 Antibody validated in Western Blot" caption="false" width="250" height="329" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against FOXA2</strong><br />Whole cell extracts from HepG2 cells were analysed by Western blot using the Diagenode antibody against FOXA2 (Cat. No. C15410343) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>',
		'label2' => 'Target Description',
		'info2' => '<p>FOXA2 (UniProtKB/Swiss-Prot entry Q9Y261) is a member of the forkhead class of transcription factors which are involved in the transcriptional activation of liver-specific genes such as AFP, albumin, tyrosine aminotransferase, PEPCK and transthyretin. FOXA2 also plays a role glucose homeostasis and in the regulation of fat metabolism and is required for the embryonic development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs. The FOXA2 gene has been linked to sporadic cases of maturity-onset diabetes of the young.</p>',
		'label3' => '',
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		'format' => '50 μg',
		'catalog_number' => 'C15410343',
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		'sf_code' => 'C15410343-D001-000581',
		'type' => 'FRE',
		'search_order' => '03-Antibody',
		'price_EUR' => '380',
		'price_USD' => '380',
		'price_GBP' => '340',
		'price_JPY' => '59525',
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		'last_datasheet_update' => 'November 21, 2017',
		'slug' => 'foxa2-polyclonal-antibody-50-ug',
		'meta_title' => 'FOXA2 polyclonal antibody',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2024-01-17 19:21:10',
		'created' => '2017-12-21 11:00:39',
		'locale' => 'jpn'
	),
	'Antibody' => array(
		'host' => '*****',
		'id' => '517',
		'name' => 'FOXA2 polyclonal antibody',
		'description' => 'FOXA2 (UniProtKB/Swiss-Prot entry Q9Y261) is a member of the forkhead class of transcription factors which are involved in the
transcriptional activation of liver-specific genes such as AFP, albumin, tyrosine aminotransferase, PEPCK and transthyretin.
FOXA2 also plays a role glucose homeostasis and in the regulation of fat metabolism and is required for the embryonic
development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs. The FOXA2 gene has been
linked to sporadic cases of maturity-onset diabetes of the young.',
		'clonality' => '',
		'isotype' => '',
		'lot' => 'A2683-0040',
		'concentration' => '2.4 μg/μl',
		'reactivity' => 'Human: positive. Other species: not tested.',
		'type' => 'Polyclonal',
		'purity' => 'Affinity purified polyclonal antibody in PBS containing 0.05% azide.',
		'classification' => '',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 &mu;g/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td>ELISA</td>
<td>1:5,000</td>
<td>Fig 3</td>
</tr>
<tr>
<td>WB</td>
<td>1:500</td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 &mu;g per IP.</small></p>',
		'storage_conditions' => 'Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
		'storage_buffer' => '',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
		'uniprot_acc' => '',
		'slug' => 'foxa2-polyclonal-antibody',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2019-09-10 14:41:10',
		'created' => '2017-12-21 10:55:25',
		'select_label' => '517 - FOXA2 polyclonal antibody (A2683-0040 - 2.4 μg/μl - Human: positive. Other species: not tested. - Affinity purified polyclonal antibody in PBS containing 0.05% azide. - Rabbit)'
	),
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			'id' => '2922',
			'antibody_id' => '517',
			'name' => 'FOXA2 Antibody',
			'description' => '<p><strong>Other names:</strong> HNF3B, TFC3B, HNF-3B, TCF-3B</p>
<p>Polyclonal antibody raised in rabbit against human <strong>FOXA2 (Forkhead Box A2)</strong>, using two synthetic peptides containing a sequence from the central part and the N-terminus of the protein, respectively.</p>',
			'label1' => 'Validation data',
			'info1' => '<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chip.jpg" alt="FOXA2 Antibody ChIP Grade" caption="false" width="432" height="328" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP assays were performed using HepG2 cells, the Diagenode antibody against FOXA2 (cat. No. C15410343) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the AFP and TTR genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-a.jpg" alt="FOXA2 Antibody ChIP-seq Grade" caption="false" width="700" height="83" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-b.jpg" alt="FOXA2 Antibody for ChIP-seq " caption="false" width="700" height="224" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-c.jpg" alt="FOXA2 Antibody for ChIP-seq assay" caption="false" width="700" height="104" /></p>
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-chipseq-d.jpg" alt="FOXA2 Antibody validated in ChIP-seq" caption="false" width="700" height="102" /></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong> Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against FOXA2</strong><br />ChIP was performed with 2 &mu;g of the Diagenode antibody against FOXA2 (Cat. No. C15410343) on sheared chromatin from 4,000,000 HepG2 cells using the &ldquo;iDeal ChIP-seq&rdquo; kit as described above. The IP&rsquo;d DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer&rsquo;s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 1.5 Mb region of the human X-chromosome (figures 2A and B), and in two genomic regions surrounding the AFP and TTR positive control genes (figure 2C and D).</small></p>
</div>
</div>
<div class="row">
<div class="small-6 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-elisa.jpg" alt="FOXA2 Antibody ELISA Validation" caption="false" width="432" height="328" /></p>
</div>
<div class="small-6 columns">
<p><small><strong> Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against FOXA2 (Cat. No. C15410343). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:176,000.</small></p>
</div>
</div>
<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/C15410343-wb.jpg" alt="FOXA2 Antibody validated in Western Blot" caption="false" width="250" height="329" /></p>
</div>
<div class="small-8 columns">
<p><small><strong> Figure 4. Western blot analysis using the Diagenode antibody directed against FOXA2</strong><br />Whole cell extracts from HepG2 cells were analysed by Western blot using the Diagenode antibody against FOXA2 (Cat. No. C15410343) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</small></p>
</div>
</div>',
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			'info2' => '<p>FOXA2 (UniProtKB/Swiss-Prot entry Q9Y261) is a member of the forkhead class of transcription factors which are involved in the transcriptional activation of liver-specific genes such as AFP, albumin, tyrosine aminotransferase, PEPCK and transthyretin. FOXA2 also plays a role glucose homeostasis and in the regulation of fat metabolism and is required for the embryonic development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs. The FOXA2 gene has been linked to sporadic cases of maturity-onset diabetes of the young.</p>',
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			'meta_description' => 'FOXA2 (Forkhead Box A2) Polyclonal Antibody validated  in ChIP-seq, ChIP-qPCR, WB and ELISA. Batch-specific data available on the website. Other names: HNF3B, TFC3B, HNF-3B, TCF-3B. Sample size available.',
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<div class="small-12 medium-12 large-12 columns">Enzyme-linked immunosorbent assay.</div>
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			'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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			'name' => 'ChIP-grade antibodies',
			'description' => '<div class="row">
<div class="small-10 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
<div class="small-2 columns"><img src="https://www.diagenode.com/emailing/images/epi-success-guaranteed-icon.png" alt="Epigenetic success guaranteed" /></div>
</div>
<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
<div class="small-12 medium-6 large-6 columns">
<p></p>
<p></p>
<p></p>
</div>
</div>
<p></p>
<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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			'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP',
			'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode',
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			'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
<div class="row">
<div class="small-12 medium-9 large-9 columns">
<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
</div>
</div>
<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'description' => '<p><span style="font-weight: 400;">The list of Diagenode&rsquo;s highly specific antibodies for transcription studies includes the antibodies against many transcription factors and nuclear receptors. &nbsp;Check the list below to see our targets.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Highly sensitive and specific</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Cost-effective (requires less antibody per reaction)</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Batch-specific data is available on the website</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Expert technical support</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Sample sizes available</span></li>
<li><span style="font-weight: 400;"> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 100% satisfaction guarantee</span></li>
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			'meta_description' => 'Diagenode offers polyclonal and monoclonal antibodies for Transcription studie',
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			'name' => 'All antibodies',
			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'modified' => '2024-01-17 19:18:32',
			'created' => '2024-01-17 19:18:32',
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			'language' => 'ja',
			'url' => 'files/SDS/FOXA2/SDS-C15410343-FOXA2_Antibody-JP-ja-GHS_2_0.pdf',
			'countries' => 'JP',
			'modified' => '2024-01-17 19:19:43',
			'created' => '2024-01-17 19:19:43',
			'ProductsSafetySheet' => array(
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		(int) 7 => array(
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			'language' => 'es',
			'url' => 'files/SDS/FOXA2/SDS-C15410343-FOXA2_Antibody-ES-es-GHS_2_0.pdf',
			'countries' => 'ES',
			'modified' => '2024-01-17 19:20:39',
			'created' => '2024-01-17 19:20:39',
			'ProductsSafetySheet' => array(
				[maximum depth reached]
			)
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	(int) 1 => 'US',
	(int) 2 => 'IE',
	(int) 3 => 'GB',
	(int) 4 => 'DK',
	(int) 5 => 'NO',
	(int) 6 => 'SE',
	(int) 7 => 'FI',
	(int) 8 => 'NL',
	(int) 9 => 'BE',
	(int) 10 => 'LU',
	(int) 11 => 'FR',
	(int) 12 => 'DE',
	(int) 13 => 'CH',
	(int) 14 => 'AT',
	(int) 15 => 'ES',
	(int) 16 => 'IT',
	(int) 17 => 'PT'
)
$outsource = true
$other_formats = array(
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		'label2' => '',
		'info2' => '',
		'label3' => '',
		'info3' => '',
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		'price_CNY' => '0',
		'price_AUD' => '288',
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		'in_stock' => false,
		'featured' => false,
		'no_promo' => false,
		'online' => true,
		'master' => false,
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			'product_id' => '2929',
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	'info2' => '',
	'label3' => '',
	'info3' => '',
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	'price_CNY' => '0',
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	'slug' => 'foxa2-polyclonal-antibody-10-ug',
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$rrbs_service = array(
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)
$chipseq_service = array(
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$labelize = object(Closure) {
	
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	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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)
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	'parent_id' => '40',
	'name' => 'WB',
	'description' => '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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	'online' => true,
	'tabular' => true,
	'slug' => 'western-blot-antibodies',
	'meta_keywords' => ' Western Blot Antibodies ,western blot protocol,Western Blotting Products,Polyclonal antibodies ,monoclonal antibodies ',
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	'meta_title' => ' Western Blot  - Monoclonal antibody - Polyclonal antibody  | Diagenode',
	'modified' => '2016-04-26 12:44:51',
	'created' => '2015-01-07 09:20:00',
	'locale' => 'jpn'
)
$description = '<p><strong>Western blot</strong> : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies.</p>
<p>Learn more about: <a href="../applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>'
$name = 'WB'
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	'name' => 'FOXA2 polyclonal antibody',
	'description' => '<p>Polyclonal antibody raised in rabbit against human FOXA2 (Forkhead Box A2), using two synthetic peptides containing a sequence from the central part and the N-terminus of the protein, respectively.</p>',
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	'type' => 'Datasheet',
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	'slug' => 'foxa2-polyclonal-antibody',
	'meta_keywords' => '',
	'meta_description' => '',
	'modified' => '2018-01-03 11:11:03',
	'created' => '2018-01-03 11:11:03',
	'ProductsDocument' => array(
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		'product_id' => '2922',
		'document_id' => '979'
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)
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	'name' => 'SDS C15410343 FOXA2 Antibody ES es',
	'language' => 'es',
	'url' => 'files/SDS/FOXA2/SDS-C15410343-FOXA2_Antibody-ES-es-GHS_2_0.pdf',
	'countries' => 'ES',
	'modified' => '2024-01-17 19:20:39',
	'created' => '2024-01-17 19:20:39',
	'ProductsSafetySheet' => array(
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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FOXA2 polyclonal antibody

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