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Figure 1. H3T6pK9me1 antibody ChIP results Chromatin Immunoprecipitation of H3T6pK9me1 antibody. Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of H3T6pK9me1 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and normalized to the input chromatin.
Figure 2. H3T6pK9me1 antibody Immunofluorescence results Immunofluorescence of H3T6pK9me1 antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody: incubated at a 1:100 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10,000 for 45 min at RT. Localization: H3T6pK9me1 is nuclear and chromosomal. Staining: H3T6pK9me1 is expressed in green and the nuclei are counterstained with DAPI (blue).
Figure 3. H3T6pK9me1 antibody Western blot results Western Blot of H3T6pK9me1 antibody. 30 μg NIH-3T3 Histone extracts. Primary antibody diluted 1:1,000 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.
Figure 4. H3T6pK9me1 antibody Western blot results Western Blot of H3T6pK9me1 antibody. 30 μg C. elegans embryo lysate. Primary antibody diluted 1:1,000 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.
Figure 5. H3T6pK9me1 antibody Western blot results Western Blot of H3T6pK9me1 antibody. 30 μg HeLa Histone extracts. Primary antibody diluted 1:1,000 overnight at 4°C. Secondary antibody: IRDye800TM rabbit secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: ~15 kDa. Other band(s): None.
Figure 6. H3T6pK9me1 antibody Dot blot results Dot Blot of H3T6pK9me1 antibody. Lane 1: Histone H3 1-13. Lane 2: T6p. Lane 3: T6pK9Me1. Lane 4: T6pK9Me2. Lane 5: T6pK9Me3. Lane 6: Histone H3 2-17. Lane 7: K9Me1. Lane 8: K9Me2. Lane 9: K9Me3. Lane 10: K9Ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody diluted 1:1,000 for 45 min at 4°C. Secondary antibody: DylightTM488 rabbit secondary antibody at 1:10,000 for 45 min at RT.
IF Immunofluorescence:
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Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9
HeLa cells transfected with a Cas9 expression vector (... Read more
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