| Datasheet Human cfos promoter pp1004 DATASHEET Datasheet description | Download |
This primer pair specifically amplifies a genomic region containing the human c-fos promoter. The primers are thoroughly tested and optimized for routine SYBR® Green Real-Time qPCR assay following ChIP and for ChIP-sequencing library validation (e.g. before and after ChIP-seq library preparation).
| Datasheet Human cfos promoter pp1004 DATASHEET Datasheet description | Download |
| c-fos Promoter Primer Pair SDS GB en | Download |
| c-fos Promoter Primer Pair SDS US en | Download |
| c-fos Promoter Primer Pair SDS DE de | Download |
| c-fos Promoter Primer Pair SDS JP ja | Download |
| c-fos Promoter Primer Pair SDS BE nl | Download |
| c-fos Promoter Primer Pair SDS BE fr | Download |
| c-fos Promoter Primer Pair SDS FR fr | Download |
| c-fos Promoter Primer Pair SDS ES es | Download |
 How to properly cite this product in your workDiagenode strongly recommends using this: Human ChIP-seq grade c-fos promoter primer pair (Diagenode Cat# C17011004-50). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Epigenetic Modifications with DZNep, NaBu and SAHA in Luminal and Mesenchymal-like Breast Cancer Subtype Cells |
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'Human ChIP-seq grade c-fos promoter primer pair', 'meta_title' => 'Human ChIP-seq grade c-fos promoter primer pair', 'product' => array( 'Product' => array( 'id' => '2517', 'antibody_id' => null, 'name' => 'Human ChIP-seq grade c-fos promoter primer pair', 'description' => '<p><span>This primer pair specifically amplifies a genomic region containing the human c-fos promoter. The primers are thoroughly tested and optimized for routine SYBR® Green Real-Time qPCR assay following ChIP and for ChIP-sequencing library validation (e.g. before and after ChIP-seq library preparation).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011004-50', 'old_catalog_number' => 'pp-1004-050', 'sf_code' => 'C17011004-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-chip-seq-grade-c-fos-promoter-primer-pair-50-ul', 'meta_title' => 'Human ChIP-seq grade c-fos promoter primer pair', 'meta_keywords' => '', 'meta_description' => 'Human ChIP-seq grade c-fos promoter primer pair', 'modified' => '2016-10-21 08:17:25', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/jp/p/human-chip-seq-grade-c-fos-promoter-primer-pair-50-ul' ) $language = 'jp' $meta_keywords = '' $meta_description = 'Human ChIP-seq grade c-fos promoter primer pair' $meta_title = 'Human ChIP-seq grade c-fos promoter primer pair' $product = array( 'Product' => array( 'id' => '2517', 'antibody_id' => null, 'name' => 'Human ChIP-seq grade c-fos promoter primer pair', 'description' => '<p><span>This primer pair specifically amplifies a genomic region containing the human c-fos promoter. The primers are thoroughly tested and optimized for routine SYBR® Green Real-Time qPCR assay following ChIP and for ChIP-sequencing library validation (e.g. before and after ChIP-seq library preparation).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011004-50', 'old_catalog_number' => 'pp-1004-050', 'sf_code' => 'C17011004-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-chip-seq-grade-c-fos-promoter-primer-pair-50-ul', 'meta_title' => 'Human ChIP-seq grade c-fos promoter primer pair', 'meta_keywords' => '', 'meta_description' => 'Human ChIP-seq grade c-fos promoter primer pair', 'modified' => '2016-10-21 08:17:25', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( 'id' => '118', 'name' => 'C17011004', 'product_id' => '2517', 'modified' => '2016-02-19 21:27:26', 'created' => '2016-02-19 21:27:26' ) ), 'Group' => array( 'Group' => array( 'id' => '118', 'name' => 'C17011004', 'product_id' => '2517', 'modified' => '2016-02-19 21:27:26', 'created' => '2016-02-19 21:27:26' ), 'Master' => array( 'id' => '2517', 'antibody_id' => null, 'name' => 'Human ChIP-seq grade c-fos promoter primer pair', 'description' => '<p><span>This primer pair specifically amplifies a genomic region containing the human c-fos promoter. The primers are thoroughly tested and optimized for routine SYBR® Green Real-Time qPCR assay following ChIP and for ChIP-sequencing library validation (e.g. before and after ChIP-seq library preparation).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011004-50', 'old_catalog_number' => 'pp-1004-050', 'sf_code' => 'C17011004-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-chip-seq-grade-c-fos-promoter-primer-pair-50-ul', 'meta_title' => 'Human ChIP-seq grade c-fos promoter primer pair', 'meta_keywords' => '', 'meta_description' => 'Human ChIP-seq grade c-fos promoter primer pair', 'modified' => '2016-10-21 08:17:25', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '69', 'position' => '1', 'parent_id' => '35', 'name' => 'Human', 'description' => '<p>Primer pairs - Human</p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'primer-pairs-human', 'cookies_tag_id' => null, 'meta_keywords' => 'Primer pairs, human, ChIP-seq grade', 'meta_description' => 'Diagenode offers Primer pairs for Human ChIP-seq grade', 'meta_title' => 'Primer pairs - Human | Diagenode', 'modified' => '2016-10-21 08:00:50', 'created' => '2015-07-23 15:38:42', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '254', 'name' => 'Datasheet Human cfos promoter pp1004', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/primer_pairs/Datasheet_Human_cfos_promoter_pp1004.pdf', 'slug' => 'datasheet-human-cfos-promoter-pp1004', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '2980', 'name' => 'Epigenetic Modifications with DZNep, NaBu and SAHA in Luminal and Mesenchymal-like Breast Cancer Subtype Cells', 'authors' => 'Dagdemir A et al.', 'description' => '<h4>BACKGROUND/AIM:</h4> <p><abstracttext label="BACKGROUND/AIM" nlmcategory="OBJECTIVE">Numerous studies have shown that breast cancer and epigenetic mechanisms have a very powerful interactive relation. The MCF7 cell line, representative of luminal subtype and the MDA-MB 231 cell line representative of mesenchymal-like subtype were treated respectively with a Histone Methyl Transferase Inhibitors (HMTi), 3-Deazaneplanocin hydrochloride (DZNep), two histone deacetylase inhibitors (HDACi), sodium butyrate (NaBu), and suberoylanilide hydroxamic acid (SAHA) for 48 h.</abstracttext></p> <h4>MATERIALS AND METHODS:</h4> <p><abstracttext label="MATERIALS AND METHODS" nlmcategory="METHODS">Chromatin immunoprecipitation (ChIP) was used to observe HDACis (SAHA and NaBu) and HMTi (DZNep) impact on histones and more specifically on H3K27me3, H3K9ac and H3K4ac marks with Q-PCR analysis of BRCA1, SRC3 and P300 genes. Furthermore, the HDACi and HMTi effects on mRNA and protein expression of BRCA1, SRC3 and P300 genes were checked. In addition, statistical analyses were used.</abstracttext></p> <h4>RESULTS:</h4> <p><abstracttext label="RESULTS" nlmcategory="RESULTS">In the MCF7 luminal subtype with positive ER, H3k4ac was significantly increased on BRCA1 with SAHA. On the contrary, in the MDA-MB 231 breast cancer cell line, representative of mesenchymal-like subtype with negative estrogen receptor, HDACis had no effect. Also, DZNEP decreased significantly H3K27me3 on BRCA1 in MDA-MB 231. Besides, on SRC3, a significant increase for H3K4ac was obtained in MCF7 treated with SAHA. And DZNEP had no effect in MCF7. Also, in MDA-MB 231 treated with DZNEP, H3K27me3 significantly decreased on SRC3 while H3K4ac was significantly increased in MDA-MB-231 treated with SAHA or NaBu for P300.</abstracttext></p> <h4>CONCLUSION:</h4> <p><abstracttext label="CONCLUSION" nlmcategory="CONCLUSIONS">Luminal and mesenchymal-like breast cancer subtype cell lines seemed to act differently to HDACis (SAHA and NaBu) or HMTi (DZNEP) treatments.</abstracttext></p>', 'date' => '2016-07-01', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/27365379', 'doi' => '', 'modified' => '2016-07-12 12:50:21', 'created' => '2016-07-12 12:46:04', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '270', 'name' => 'c-fos Promoter Primer Pair SDS GB en', 'language' => 'en', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-GB-en-GHS_1_0.pdf', 'countries' => 'GB', 'modified' => '2020-06-09 10:33:07', 'created' => '2020-06-09 10:33:07', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '272', 'name' => 'c-fos Promoter Primer Pair SDS US en', 'language' => 'en', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-US-en-GHS_1_0.pdf', 'countries' => 'US', 'modified' => '2020-06-09 10:34:22', 'created' => '2020-06-09 10:34:22', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '267', 'name' => 'c-fos Promoter Primer Pair SDS DE de', 'language' => 'de', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-DE-de-GHS_1_0.pdf', 'countries' => 'DE', 'modified' => '2020-06-09 10:27:14', 'created' => '2020-06-09 10:27:14', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '271', 'name' => 'c-fos Promoter Primer Pair SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-JP-ja-GHS_2_0.pdf', 'countries' => 'JP', 'modified' => '2020-06-09 10:33:45', 'created' => '2020-06-09 10:33:45', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '266', 'name' => 'c-fos Promoter Primer Pair SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-BE-nl-GHS_1_0.pdf', 'countries' => 'BE', 'modified' => '2020-06-09 10:25:59', 'created' => '2020-06-09 10:25:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '265', 'name' => 'c-fos Promoter Primer Pair SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-BE-fr-GHS_1_0.pdf', 'countries' => 'BE', 'modified' => '2020-06-09 10:24:34', 'created' => '2020-06-09 10:24:34', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '269', 'name' => 'c-fos Promoter Primer Pair SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-FR-fr-GHS_1_0.pdf', 'countries' => 'FR', 'modified' => '2020-06-09 10:29:06', 'created' => '2020-06-09 10:29:06', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '268', 'name' => 'c-fos Promoter Primer Pair SDS ES es', 'language' => 'es', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-ES-es-GHS_1_0.pdf', 'countries' => 'ES', 'modified' => '2020-06-09 10:28:17', 'created' => '2020-06-09 10:28:17', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/jp/p/human-chip-seq-grade-c-fos-promoter-primer-pair-50-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '2518', 'antibody_id' => null, 'name' => 'Human ChIP-seq grade c-fos promoter primer pair', 'description' => '<p><span>This primer pair specifically amplifies a genomic region containing the human c-fos promoter. The primers are thoroughly tested and optimized for routine SYBR® Green Real-Time qPCR assay following ChIP and for ChIP-sequencing library validation (e.g. before and after ChIP-seq library preparation).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011004-500', 'old_catalog_number' => 'pp-1004-500', 'sf_code' => 'C17011004-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-chip-seq-grade-c-fos-promoter-primer-pair-500-ul', 'meta_title' => 'Human ChIP-seq grade c-fos promoter primer pair', 'meta_keywords' => '', 'meta_description' => 'Human ChIP-seq grade c-fos promoter primer pair', 'modified' => '2016-02-19 21:27:54', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '129', 'product_id' => '2518', 'group_id' => '118' ) ) ) $pro = array( 'id' => '2518', 'antibody_id' => null, 'name' => 'Human ChIP-seq grade c-fos promoter primer pair', 'description' => '<p><span>This primer pair specifically amplifies a genomic region containing the human c-fos promoter. The primers are thoroughly tested and optimized for routine SYBR® Green Real-Time qPCR assay following ChIP and for ChIP-sequencing library validation (e.g. before and after ChIP-seq library preparation).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011004-500', 'old_catalog_number' => 'pp-1004-500', 'sf_code' => 'C17011004-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-chip-seq-grade-c-fos-promoter-primer-pair-500-ul', 'meta_title' => 'Human ChIP-seq grade c-fos promoter primer pair', 'meta_keywords' => '', 'meta_description' => 'Human ChIP-seq grade c-fos promoter primer pair', 'modified' => '2016-02-19 21:27:54', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '129', 'product_id' => '2518', 'group_id' => '118' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = ' <span style="color:#CCC">(pp-1004-500)</span>' $country_code = 'US' $other_format = array( 'id' => '2518', 'antibody_id' => null, 'name' => 'Human ChIP-seq grade c-fos promoter primer pair', 'description' => '<p><span>This primer pair specifically amplifies a genomic region containing the human c-fos promoter. The primers are thoroughly tested and optimized for routine SYBR® Green Real-Time qPCR assay following ChIP and for ChIP-sequencing library validation (e.g. before and after ChIP-seq library preparation).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011004-500', 'old_catalog_number' => 'pp-1004-500', 'sf_code' => 'C17011004-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-chip-seq-grade-c-fos-promoter-primer-pair-500-ul', 'meta_title' => 'Human ChIP-seq grade c-fos promoter primer pair', 'meta_keywords' => '', 'meta_description' => 'Human ChIP-seq grade c-fos promoter primer pair', 'modified' => '2016-02-19 21:27:54', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '129', 'product_id' => '2518', 'group_id' => '118' ) ) $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '254', 'name' => 'Datasheet Human cfos promoter pp1004', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/primer_pairs/Datasheet_Human_cfos_promoter_pp1004.pdf', 'slug' => 'datasheet-human-cfos-promoter-pp1004', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( 'id' => '586', 'product_id' => '2517', 'document_id' => '254' ) ) $sds = array( 'id' => '268', 'name' => 'c-fos Promoter Primer Pair SDS ES es', 'language' => 'es', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-ES-es-GHS_1_0.pdf', 'countries' => 'ES', 'modified' => '2020-06-09 10:28:17', 'created' => '2020-06-09 10:28:17', 'ProductsSafetySheet' => array( 'id' => '502', 'product_id' => '2517', 'safety_sheet_id' => '268' ) ) $publication = array( 'id' => '2980', 'name' => 'Epigenetic Modifications with DZNep, NaBu and SAHA in Luminal and Mesenchymal-like Breast Cancer Subtype Cells', 'authors' => 'Dagdemir A et al.', 'description' => '<h4>BACKGROUND/AIM:</h4> <p><abstracttext label="BACKGROUND/AIM" nlmcategory="OBJECTIVE">Numerous studies have shown that breast cancer and epigenetic mechanisms have a very powerful interactive relation. The MCF7 cell line, representative of luminal subtype and the MDA-MB 231 cell line representative of mesenchymal-like subtype were treated respectively with a Histone Methyl Transferase Inhibitors (HMTi), 3-Deazaneplanocin hydrochloride (DZNep), two histone deacetylase inhibitors (HDACi), sodium butyrate (NaBu), and suberoylanilide hydroxamic acid (SAHA) for 48 h.</abstracttext></p> <h4>MATERIALS AND METHODS:</h4> <p><abstracttext label="MATERIALS AND METHODS" nlmcategory="METHODS">Chromatin immunoprecipitation (ChIP) was used to observe HDACis (SAHA and NaBu) and HMTi (DZNep) impact on histones and more specifically on H3K27me3, H3K9ac and H3K4ac marks with Q-PCR analysis of BRCA1, SRC3 and P300 genes. Furthermore, the HDACi and HMTi effects on mRNA and protein expression of BRCA1, SRC3 and P300 genes were checked. In addition, statistical analyses were used.</abstracttext></p> <h4>RESULTS:</h4> <p><abstracttext label="RESULTS" nlmcategory="RESULTS">In the MCF7 luminal subtype with positive ER, H3k4ac was significantly increased on BRCA1 with SAHA. On the contrary, in the MDA-MB 231 breast cancer cell line, representative of mesenchymal-like subtype with negative estrogen receptor, HDACis had no effect. Also, DZNEP decreased significantly H3K27me3 on BRCA1 in MDA-MB 231. Besides, on SRC3, a significant increase for H3K4ac was obtained in MCF7 treated with SAHA. And DZNEP had no effect in MCF7. Also, in MDA-MB 231 treated with DZNEP, H3K27me3 significantly decreased on SRC3 while H3K4ac was significantly increased in MDA-MB-231 treated with SAHA or NaBu for P300.</abstracttext></p> <h4>CONCLUSION:</h4> <p><abstracttext label="CONCLUSION" nlmcategory="CONCLUSIONS">Luminal and mesenchymal-like breast cancer subtype cell lines seemed to act differently to HDACis (SAHA and NaBu) or HMTi (DZNEP) treatments.</abstracttext></p>', 'date' => '2016-07-01', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/27365379', 'doi' => '', 'modified' => '2016-07-12 12:50:21', 'created' => '2016-07-12 12:46:04', 'ProductsPublication' => array( 'id' => '1434', 'product_id' => '2517', 'publication_id' => '2980' ) ) $externalLink = ' <a href="http://www.ncbi.nlm.nih.gov/pubmed/27365379" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: header [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'Human ChIP-seq grade c-fos promoter primer pair', 'meta_title' => 'Human ChIP-seq grade c-fos promoter primer pair', 'product' => array( 'Product' => array( 'id' => '2517', 'antibody_id' => null, 'name' => 'Human ChIP-seq grade c-fos promoter primer pair', 'description' => '<p><span>This primer pair specifically amplifies a genomic region containing the human c-fos promoter. The primers are thoroughly tested and optimized for routine SYBR® Green Real-Time qPCR assay following ChIP and for ChIP-sequencing library validation (e.g. before and after ChIP-seq library preparation).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011004-50', 'old_catalog_number' => 'pp-1004-050', 'sf_code' => 'C17011004-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-chip-seq-grade-c-fos-promoter-primer-pair-50-ul', 'meta_title' => 'Human ChIP-seq grade c-fos promoter primer pair', 'meta_keywords' => '', 'meta_description' => 'Human ChIP-seq grade c-fos promoter primer pair', 'modified' => '2016-10-21 08:17:25', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/jp/p/human-chip-seq-grade-c-fos-promoter-primer-pair-50-ul' ) $language = 'jp' $meta_keywords = '' $meta_description = 'Human ChIP-seq grade c-fos promoter primer pair' $meta_title = 'Human ChIP-seq grade c-fos promoter primer pair' $product = array( 'Product' => array( 'id' => '2517', 'antibody_id' => null, 'name' => 'Human ChIP-seq grade c-fos promoter primer pair', 'description' => '<p><span>This primer pair specifically amplifies a genomic region containing the human c-fos promoter. The primers are thoroughly tested and optimized for routine SYBR® Green Real-Time qPCR assay following ChIP and for ChIP-sequencing library validation (e.g. before and after ChIP-seq library preparation).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011004-50', 'old_catalog_number' => 'pp-1004-050', 'sf_code' => 'C17011004-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-chip-seq-grade-c-fos-promoter-primer-pair-50-ul', 'meta_title' => 'Human ChIP-seq grade c-fos promoter primer pair', 'meta_keywords' => '', 'meta_description' => 'Human ChIP-seq grade c-fos promoter primer pair', 'modified' => '2016-10-21 08:17:25', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( 'id' => '118', 'name' => 'C17011004', 'product_id' => '2517', 'modified' => '2016-02-19 21:27:26', 'created' => '2016-02-19 21:27:26' ) ), 'Group' => array( 'Group' => array( 'id' => '118', 'name' => 'C17011004', 'product_id' => '2517', 'modified' => '2016-02-19 21:27:26', 'created' => '2016-02-19 21:27:26' ), 'Master' => array( 'id' => '2517', 'antibody_id' => null, 'name' => 'Human ChIP-seq grade c-fos promoter primer pair', 'description' => '<p><span>This primer pair specifically amplifies a genomic region containing the human c-fos promoter. The primers are thoroughly tested and optimized for routine SYBR® Green Real-Time qPCR assay following ChIP and for ChIP-sequencing library validation (e.g. before and after ChIP-seq library preparation).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011004-50', 'old_catalog_number' => 'pp-1004-050', 'sf_code' => 'C17011004-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-chip-seq-grade-c-fos-promoter-primer-pair-50-ul', 'meta_title' => 'Human ChIP-seq grade c-fos promoter primer pair', 'meta_keywords' => '', 'meta_description' => 'Human ChIP-seq grade c-fos promoter primer pair', 'modified' => '2016-10-21 08:17:25', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '69', 'position' => '1', 'parent_id' => '35', 'name' => 'Human', 'description' => '<p>Primer pairs - Human</p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'primer-pairs-human', 'cookies_tag_id' => null, 'meta_keywords' => 'Primer pairs, human, ChIP-seq grade', 'meta_description' => 'Diagenode offers Primer pairs for Human ChIP-seq grade', 'meta_title' => 'Primer pairs - Human | Diagenode', 'modified' => '2016-10-21 08:00:50', 'created' => '2015-07-23 15:38:42', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '254', 'name' => 'Datasheet Human cfos promoter pp1004', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/primer_pairs/Datasheet_Human_cfos_promoter_pp1004.pdf', 'slug' => 'datasheet-human-cfos-promoter-pp1004', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '2980', 'name' => 'Epigenetic Modifications with DZNep, NaBu and SAHA in Luminal and Mesenchymal-like Breast Cancer Subtype Cells', 'authors' => 'Dagdemir A et al.', 'description' => '<h4>BACKGROUND/AIM:</h4> <p><abstracttext label="BACKGROUND/AIM" nlmcategory="OBJECTIVE">Numerous studies have shown that breast cancer and epigenetic mechanisms have a very powerful interactive relation. The MCF7 cell line, representative of luminal subtype and the MDA-MB 231 cell line representative of mesenchymal-like subtype were treated respectively with a Histone Methyl Transferase Inhibitors (HMTi), 3-Deazaneplanocin hydrochloride (DZNep), two histone deacetylase inhibitors (HDACi), sodium butyrate (NaBu), and suberoylanilide hydroxamic acid (SAHA) for 48 h.</abstracttext></p> <h4>MATERIALS AND METHODS:</h4> <p><abstracttext label="MATERIALS AND METHODS" nlmcategory="METHODS">Chromatin immunoprecipitation (ChIP) was used to observe HDACis (SAHA and NaBu) and HMTi (DZNep) impact on histones and more specifically on H3K27me3, H3K9ac and H3K4ac marks with Q-PCR analysis of BRCA1, SRC3 and P300 genes. Furthermore, the HDACi and HMTi effects on mRNA and protein expression of BRCA1, SRC3 and P300 genes were checked. In addition, statistical analyses were used.</abstracttext></p> <h4>RESULTS:</h4> <p><abstracttext label="RESULTS" nlmcategory="RESULTS">In the MCF7 luminal subtype with positive ER, H3k4ac was significantly increased on BRCA1 with SAHA. On the contrary, in the MDA-MB 231 breast cancer cell line, representative of mesenchymal-like subtype with negative estrogen receptor, HDACis had no effect. Also, DZNEP decreased significantly H3K27me3 on BRCA1 in MDA-MB 231. Besides, on SRC3, a significant increase for H3K4ac was obtained in MCF7 treated with SAHA. And DZNEP had no effect in MCF7. Also, in MDA-MB 231 treated with DZNEP, H3K27me3 significantly decreased on SRC3 while H3K4ac was significantly increased in MDA-MB-231 treated with SAHA or NaBu for P300.</abstracttext></p> <h4>CONCLUSION:</h4> <p><abstracttext label="CONCLUSION" nlmcategory="CONCLUSIONS">Luminal and mesenchymal-like breast cancer subtype cell lines seemed to act differently to HDACis (SAHA and NaBu) or HMTi (DZNEP) treatments.</abstracttext></p>', 'date' => '2016-07-01', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/27365379', 'doi' => '', 'modified' => '2016-07-12 12:50:21', 'created' => '2016-07-12 12:46:04', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '270', 'name' => 'c-fos Promoter Primer Pair SDS GB en', 'language' => 'en', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-GB-en-GHS_1_0.pdf', 'countries' => 'GB', 'modified' => '2020-06-09 10:33:07', 'created' => '2020-06-09 10:33:07', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '272', 'name' => 'c-fos Promoter Primer Pair SDS US en', 'language' => 'en', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-US-en-GHS_1_0.pdf', 'countries' => 'US', 'modified' => '2020-06-09 10:34:22', 'created' => '2020-06-09 10:34:22', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '267', 'name' => 'c-fos Promoter Primer Pair SDS DE de', 'language' => 'de', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-DE-de-GHS_1_0.pdf', 'countries' => 'DE', 'modified' => '2020-06-09 10:27:14', 'created' => '2020-06-09 10:27:14', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '271', 'name' => 'c-fos Promoter Primer Pair SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-JP-ja-GHS_2_0.pdf', 'countries' => 'JP', 'modified' => '2020-06-09 10:33:45', 'created' => '2020-06-09 10:33:45', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '266', 'name' => 'c-fos Promoter Primer Pair SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-BE-nl-GHS_1_0.pdf', 'countries' => 'BE', 'modified' => '2020-06-09 10:25:59', 'created' => '2020-06-09 10:25:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '265', 'name' => 'c-fos Promoter Primer Pair SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-BE-fr-GHS_1_0.pdf', 'countries' => 'BE', 'modified' => '2020-06-09 10:24:34', 'created' => '2020-06-09 10:24:34', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '269', 'name' => 'c-fos Promoter Primer Pair SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-FR-fr-GHS_1_0.pdf', 'countries' => 'FR', 'modified' => '2020-06-09 10:29:06', 'created' => '2020-06-09 10:29:06', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '268', 'name' => 'c-fos Promoter Primer Pair SDS ES es', 'language' => 'es', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-ES-es-GHS_1_0.pdf', 'countries' => 'ES', 'modified' => '2020-06-09 10:28:17', 'created' => '2020-06-09 10:28:17', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/jp/p/human-chip-seq-grade-c-fos-promoter-primer-pair-50-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '2518', 'antibody_id' => null, 'name' => 'Human ChIP-seq grade c-fos promoter primer pair', 'description' => '<p><span>This primer pair specifically amplifies a genomic region containing the human c-fos promoter. The primers are thoroughly tested and optimized for routine SYBR® Green Real-Time qPCR assay following ChIP and for ChIP-sequencing library validation (e.g. before and after ChIP-seq library preparation).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011004-500', 'old_catalog_number' => 'pp-1004-500', 'sf_code' => 'C17011004-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-chip-seq-grade-c-fos-promoter-primer-pair-500-ul', 'meta_title' => 'Human ChIP-seq grade c-fos promoter primer pair', 'meta_keywords' => '', 'meta_description' => 'Human ChIP-seq grade c-fos promoter primer pair', 'modified' => '2016-02-19 21:27:54', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '129', 'product_id' => '2518', 'group_id' => '118' ) ) ) $pro = array( 'id' => '2518', 'antibody_id' => null, 'name' => 'Human ChIP-seq grade c-fos promoter primer pair', 'description' => '<p><span>This primer pair specifically amplifies a genomic region containing the human c-fos promoter. The primers are thoroughly tested and optimized for routine SYBR® Green Real-Time qPCR assay following ChIP and for ChIP-sequencing library validation (e.g. before and after ChIP-seq library preparation).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011004-500', 'old_catalog_number' => 'pp-1004-500', 'sf_code' => 'C17011004-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-chip-seq-grade-c-fos-promoter-primer-pair-500-ul', 'meta_title' => 'Human ChIP-seq grade c-fos promoter primer pair', 'meta_keywords' => '', 'meta_description' => 'Human ChIP-seq grade c-fos promoter primer pair', 'modified' => '2016-02-19 21:27:54', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '129', 'product_id' => '2518', 'group_id' => '118' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = ' <span style="color:#CCC">(pp-1004-500)</span>' $country_code = 'US' $other_format = array( 'id' => '2518', 'antibody_id' => null, 'name' => 'Human ChIP-seq grade c-fos promoter primer pair', 'description' => '<p><span>This primer pair specifically amplifies a genomic region containing the human c-fos promoter. The primers are thoroughly tested and optimized for routine SYBR® Green Real-Time qPCR assay following ChIP and for ChIP-sequencing library validation (e.g. before and after ChIP-seq library preparation).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011004-500', 'old_catalog_number' => 'pp-1004-500', 'sf_code' => 'C17011004-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-chip-seq-grade-c-fos-promoter-primer-pair-500-ul', 'meta_title' => 'Human ChIP-seq grade c-fos promoter primer pair', 'meta_keywords' => '', 'meta_description' => 'Human ChIP-seq grade c-fos promoter primer pair', 'modified' => '2016-02-19 21:27:54', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '129', 'product_id' => '2518', 'group_id' => '118' ) ) $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '254', 'name' => 'Datasheet Human cfos promoter pp1004', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/primer_pairs/Datasheet_Human_cfos_promoter_pp1004.pdf', 'slug' => 'datasheet-human-cfos-promoter-pp1004', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( 'id' => '586', 'product_id' => '2517', 'document_id' => '254' ) ) $sds = array( 'id' => '268', 'name' => 'c-fos Promoter Primer Pair SDS ES es', 'language' => 'es', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-ES-es-GHS_1_0.pdf', 'countries' => 'ES', 'modified' => '2020-06-09 10:28:17', 'created' => '2020-06-09 10:28:17', 'ProductsSafetySheet' => array( 'id' => '502', 'product_id' => '2517', 'safety_sheet_id' => '268' ) ) $publication = array( 'id' => '2980', 'name' => 'Epigenetic Modifications with DZNep, NaBu and SAHA in Luminal and Mesenchymal-like Breast Cancer Subtype Cells', 'authors' => 'Dagdemir A et al.', 'description' => '<h4>BACKGROUND/AIM:</h4> <p><abstracttext label="BACKGROUND/AIM" nlmcategory="OBJECTIVE">Numerous studies have shown that breast cancer and epigenetic mechanisms have a very powerful interactive relation. The MCF7 cell line, representative of luminal subtype and the MDA-MB 231 cell line representative of mesenchymal-like subtype were treated respectively with a Histone Methyl Transferase Inhibitors (HMTi), 3-Deazaneplanocin hydrochloride (DZNep), two histone deacetylase inhibitors (HDACi), sodium butyrate (NaBu), and suberoylanilide hydroxamic acid (SAHA) for 48 h.</abstracttext></p> <h4>MATERIALS AND METHODS:</h4> <p><abstracttext label="MATERIALS AND METHODS" nlmcategory="METHODS">Chromatin immunoprecipitation (ChIP) was used to observe HDACis (SAHA and NaBu) and HMTi (DZNep) impact on histones and more specifically on H3K27me3, H3K9ac and H3K4ac marks with Q-PCR analysis of BRCA1, SRC3 and P300 genes. Furthermore, the HDACi and HMTi effects on mRNA and protein expression of BRCA1, SRC3 and P300 genes were checked. In addition, statistical analyses were used.</abstracttext></p> <h4>RESULTS:</h4> <p><abstracttext label="RESULTS" nlmcategory="RESULTS">In the MCF7 luminal subtype with positive ER, H3k4ac was significantly increased on BRCA1 with SAHA. On the contrary, in the MDA-MB 231 breast cancer cell line, representative of mesenchymal-like subtype with negative estrogen receptor, HDACis had no effect. Also, DZNEP decreased significantly H3K27me3 on BRCA1 in MDA-MB 231. Besides, on SRC3, a significant increase for H3K4ac was obtained in MCF7 treated with SAHA. And DZNEP had no effect in MCF7. Also, in MDA-MB 231 treated with DZNEP, H3K27me3 significantly decreased on SRC3 while H3K4ac was significantly increased in MDA-MB-231 treated with SAHA or NaBu for P300.</abstracttext></p> <h4>CONCLUSION:</h4> <p><abstracttext label="CONCLUSION" nlmcategory="CONCLUSIONS">Luminal and mesenchymal-like breast cancer subtype cell lines seemed to act differently to HDACis (SAHA and NaBu) or HMTi (DZNEP) treatments.</abstracttext></p>', 'date' => '2016-07-01', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/27365379', 'doi' => '', 'modified' => '2016-07-12 12:50:21', 'created' => '2016-07-12 12:46:04', 'ProductsPublication' => array( 'id' => '1434', 'product_id' => '2517', 'publication_id' => '2980' ) ) $externalLink = ' <a href="http://www.ncbi.nlm.nih.gov/pubmed/27365379" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: message [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'Human ChIP-seq grade c-fos promoter primer pair', 'meta_title' => 'Human ChIP-seq grade c-fos promoter primer pair', 'product' => array( 'Product' => array( 'id' => '2517', 'antibody_id' => null, 'name' => 'Human ChIP-seq grade c-fos promoter primer pair', 'description' => '<p><span>This primer pair specifically amplifies a genomic region containing the human c-fos promoter. The primers are thoroughly tested and optimized for routine SYBR® Green Real-Time qPCR assay following ChIP and for ChIP-sequencing library validation (e.g. before and after ChIP-seq library preparation).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011004-50', 'old_catalog_number' => 'pp-1004-050', 'sf_code' => 'C17011004-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-chip-seq-grade-c-fos-promoter-primer-pair-50-ul', 'meta_title' => 'Human ChIP-seq grade c-fos promoter primer pair', 'meta_keywords' => '', 'meta_description' => 'Human ChIP-seq grade c-fos promoter primer pair', 'modified' => '2016-10-21 08:17:25', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/jp/p/human-chip-seq-grade-c-fos-promoter-primer-pair-50-ul' ) $language = 'jp' $meta_keywords = '' $meta_description = 'Human ChIP-seq grade c-fos promoter primer pair' $meta_title = 'Human ChIP-seq grade c-fos promoter primer pair' $product = array( 'Product' => array( 'id' => '2517', 'antibody_id' => null, 'name' => 'Human ChIP-seq grade c-fos promoter primer pair', 'description' => '<p><span>This primer pair specifically amplifies a genomic region containing the human c-fos promoter. The primers are thoroughly tested and optimized for routine SYBR® Green Real-Time qPCR assay following ChIP and for ChIP-sequencing library validation (e.g. before and after ChIP-seq library preparation).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011004-50', 'old_catalog_number' => 'pp-1004-050', 'sf_code' => 'C17011004-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-chip-seq-grade-c-fos-promoter-primer-pair-50-ul', 'meta_title' => 'Human ChIP-seq grade c-fos promoter primer pair', 'meta_keywords' => '', 'meta_description' => 'Human ChIP-seq grade c-fos promoter primer pair', 'modified' => '2016-10-21 08:17:25', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( 'id' => '118', 'name' => 'C17011004', 'product_id' => '2517', 'modified' => '2016-02-19 21:27:26', 'created' => '2016-02-19 21:27:26' ) ), 'Group' => array( 'Group' => array( 'id' => '118', 'name' => 'C17011004', 'product_id' => '2517', 'modified' => '2016-02-19 21:27:26', 'created' => '2016-02-19 21:27:26' ), 'Master' => array( 'id' => '2517', 'antibody_id' => null, 'name' => 'Human ChIP-seq grade c-fos promoter primer pair', 'description' => '<p><span>This primer pair specifically amplifies a genomic region containing the human c-fos promoter. The primers are thoroughly tested and optimized for routine SYBR® Green Real-Time qPCR assay following ChIP and for ChIP-sequencing library validation (e.g. before and after ChIP-seq library preparation).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011004-50', 'old_catalog_number' => 'pp-1004-050', 'sf_code' => 'C17011004-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-chip-seq-grade-c-fos-promoter-primer-pair-50-ul', 'meta_title' => 'Human ChIP-seq grade c-fos promoter primer pair', 'meta_keywords' => '', 'meta_description' => 'Human ChIP-seq grade c-fos promoter primer pair', 'modified' => '2016-10-21 08:17:25', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '69', 'position' => '1', 'parent_id' => '35', 'name' => 'Human', 'description' => '<p>Primer pairs - Human</p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'primer-pairs-human', 'cookies_tag_id' => null, 'meta_keywords' => 'Primer pairs, human, ChIP-seq grade', 'meta_description' => 'Diagenode offers Primer pairs for Human ChIP-seq grade', 'meta_title' => 'Primer pairs - Human | Diagenode', 'modified' => '2016-10-21 08:00:50', 'created' => '2015-07-23 15:38:42', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '254', 'name' => 'Datasheet Human cfos promoter pp1004', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/primer_pairs/Datasheet_Human_cfos_promoter_pp1004.pdf', 'slug' => 'datasheet-human-cfos-promoter-pp1004', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '2980', 'name' => 'Epigenetic Modifications with DZNep, NaBu and SAHA in Luminal and Mesenchymal-like Breast Cancer Subtype Cells', 'authors' => 'Dagdemir A et al.', 'description' => '<h4>BACKGROUND/AIM:</h4> <p><abstracttext label="BACKGROUND/AIM" nlmcategory="OBJECTIVE">Numerous studies have shown that breast cancer and epigenetic mechanisms have a very powerful interactive relation. The MCF7 cell line, representative of luminal subtype and the MDA-MB 231 cell line representative of mesenchymal-like subtype were treated respectively with a Histone Methyl Transferase Inhibitors (HMTi), 3-Deazaneplanocin hydrochloride (DZNep), two histone deacetylase inhibitors (HDACi), sodium butyrate (NaBu), and suberoylanilide hydroxamic acid (SAHA) for 48 h.</abstracttext></p> <h4>MATERIALS AND METHODS:</h4> <p><abstracttext label="MATERIALS AND METHODS" nlmcategory="METHODS">Chromatin immunoprecipitation (ChIP) was used to observe HDACis (SAHA and NaBu) and HMTi (DZNep) impact on histones and more specifically on H3K27me3, H3K9ac and H3K4ac marks with Q-PCR analysis of BRCA1, SRC3 and P300 genes. Furthermore, the HDACi and HMTi effects on mRNA and protein expression of BRCA1, SRC3 and P300 genes were checked. In addition, statistical analyses were used.</abstracttext></p> <h4>RESULTS:</h4> <p><abstracttext label="RESULTS" nlmcategory="RESULTS">In the MCF7 luminal subtype with positive ER, H3k4ac was significantly increased on BRCA1 with SAHA. On the contrary, in the MDA-MB 231 breast cancer cell line, representative of mesenchymal-like subtype with negative estrogen receptor, HDACis had no effect. Also, DZNEP decreased significantly H3K27me3 on BRCA1 in MDA-MB 231. Besides, on SRC3, a significant increase for H3K4ac was obtained in MCF7 treated with SAHA. And DZNEP had no effect in MCF7. Also, in MDA-MB 231 treated with DZNEP, H3K27me3 significantly decreased on SRC3 while H3K4ac was significantly increased in MDA-MB-231 treated with SAHA or NaBu for P300.</abstracttext></p> <h4>CONCLUSION:</h4> <p><abstracttext label="CONCLUSION" nlmcategory="CONCLUSIONS">Luminal and mesenchymal-like breast cancer subtype cell lines seemed to act differently to HDACis (SAHA and NaBu) or HMTi (DZNEP) treatments.</abstracttext></p>', 'date' => '2016-07-01', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/27365379', 'doi' => '', 'modified' => '2016-07-12 12:50:21', 'created' => '2016-07-12 12:46:04', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '270', 'name' => 'c-fos Promoter Primer Pair SDS GB en', 'language' => 'en', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-GB-en-GHS_1_0.pdf', 'countries' => 'GB', 'modified' => '2020-06-09 10:33:07', 'created' => '2020-06-09 10:33:07', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '272', 'name' => 'c-fos Promoter Primer Pair SDS US en', 'language' => 'en', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-US-en-GHS_1_0.pdf', 'countries' => 'US', 'modified' => '2020-06-09 10:34:22', 'created' => '2020-06-09 10:34:22', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '267', 'name' => 'c-fos Promoter Primer Pair SDS DE de', 'language' => 'de', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-DE-de-GHS_1_0.pdf', 'countries' => 'DE', 'modified' => '2020-06-09 10:27:14', 'created' => '2020-06-09 10:27:14', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '271', 'name' => 'c-fos Promoter Primer Pair SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-JP-ja-GHS_2_0.pdf', 'countries' => 'JP', 'modified' => '2020-06-09 10:33:45', 'created' => '2020-06-09 10:33:45', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '266', 'name' => 'c-fos Promoter Primer Pair SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-BE-nl-GHS_1_0.pdf', 'countries' => 'BE', 'modified' => '2020-06-09 10:25:59', 'created' => '2020-06-09 10:25:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '265', 'name' => 'c-fos Promoter Primer Pair SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-BE-fr-GHS_1_0.pdf', 'countries' => 'BE', 'modified' => '2020-06-09 10:24:34', 'created' => '2020-06-09 10:24:34', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '269', 'name' => 'c-fos Promoter Primer Pair SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-FR-fr-GHS_1_0.pdf', 'countries' => 'FR', 'modified' => '2020-06-09 10:29:06', 'created' => '2020-06-09 10:29:06', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '268', 'name' => 'c-fos Promoter Primer Pair SDS ES es', 'language' => 'es', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-ES-es-GHS_1_0.pdf', 'countries' => 'ES', 'modified' => '2020-06-09 10:28:17', 'created' => '2020-06-09 10:28:17', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/jp/p/human-chip-seq-grade-c-fos-promoter-primer-pair-50-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '2518', 'antibody_id' => null, 'name' => 'Human ChIP-seq grade c-fos promoter primer pair', 'description' => '<p><span>This primer pair specifically amplifies a genomic region containing the human c-fos promoter. The primers are thoroughly tested and optimized for routine SYBR® Green Real-Time qPCR assay following ChIP and for ChIP-sequencing library validation (e.g. before and after ChIP-seq library preparation).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011004-500', 'old_catalog_number' => 'pp-1004-500', 'sf_code' => 'C17011004-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-chip-seq-grade-c-fos-promoter-primer-pair-500-ul', 'meta_title' => 'Human ChIP-seq grade c-fos promoter primer pair', 'meta_keywords' => '', 'meta_description' => 'Human ChIP-seq grade c-fos promoter primer pair', 'modified' => '2016-02-19 21:27:54', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '129', 'product_id' => '2518', 'group_id' => '118' ) ) ) $pro = array( 'id' => '2518', 'antibody_id' => null, 'name' => 'Human ChIP-seq grade c-fos promoter primer pair', 'description' => '<p><span>This primer pair specifically amplifies a genomic region containing the human c-fos promoter. The primers are thoroughly tested and optimized for routine SYBR® Green Real-Time qPCR assay following ChIP and for ChIP-sequencing library validation (e.g. before and after ChIP-seq library preparation).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011004-500', 'old_catalog_number' => 'pp-1004-500', 'sf_code' => 'C17011004-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-chip-seq-grade-c-fos-promoter-primer-pair-500-ul', 'meta_title' => 'Human ChIP-seq grade c-fos promoter primer pair', 'meta_keywords' => '', 'meta_description' => 'Human ChIP-seq grade c-fos promoter primer pair', 'modified' => '2016-02-19 21:27:54', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '129', 'product_id' => '2518', 'group_id' => '118' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = ' <span style="color:#CCC">(pp-1004-500)</span>' $country_code = 'US' $other_format = array( 'id' => '2518', 'antibody_id' => null, 'name' => 'Human ChIP-seq grade c-fos promoter primer pair', 'description' => '<p><span>This primer pair specifically amplifies a genomic region containing the human c-fos promoter. The primers are thoroughly tested and optimized for routine SYBR® Green Real-Time qPCR assay following ChIP and for ChIP-sequencing library validation (e.g. before and after ChIP-seq library preparation).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011004-500', 'old_catalog_number' => 'pp-1004-500', 'sf_code' => 'C17011004-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-chip-seq-grade-c-fos-promoter-primer-pair-500-ul', 'meta_title' => 'Human ChIP-seq grade c-fos promoter primer pair', 'meta_keywords' => '', 'meta_description' => 'Human ChIP-seq grade c-fos promoter primer pair', 'modified' => '2016-02-19 21:27:54', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '129', 'product_id' => '2518', 'group_id' => '118' ) ) $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '254', 'name' => 'Datasheet Human cfos promoter pp1004', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/primer_pairs/Datasheet_Human_cfos_promoter_pp1004.pdf', 'slug' => 'datasheet-human-cfos-promoter-pp1004', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( 'id' => '586', 'product_id' => '2517', 'document_id' => '254' ) ) $sds = array( 'id' => '268', 'name' => 'c-fos Promoter Primer Pair SDS ES es', 'language' => 'es', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-ES-es-GHS_1_0.pdf', 'countries' => 'ES', 'modified' => '2020-06-09 10:28:17', 'created' => '2020-06-09 10:28:17', 'ProductsSafetySheet' => array( 'id' => '502', 'product_id' => '2517', 'safety_sheet_id' => '268' ) ) $publication = array( 'id' => '2980', 'name' => 'Epigenetic Modifications with DZNep, NaBu and SAHA in Luminal and Mesenchymal-like Breast Cancer Subtype Cells', 'authors' => 'Dagdemir A et al.', 'description' => '<h4>BACKGROUND/AIM:</h4> <p><abstracttext label="BACKGROUND/AIM" nlmcategory="OBJECTIVE">Numerous studies have shown that breast cancer and epigenetic mechanisms have a very powerful interactive relation. The MCF7 cell line, representative of luminal subtype and the MDA-MB 231 cell line representative of mesenchymal-like subtype were treated respectively with a Histone Methyl Transferase Inhibitors (HMTi), 3-Deazaneplanocin hydrochloride (DZNep), two histone deacetylase inhibitors (HDACi), sodium butyrate (NaBu), and suberoylanilide hydroxamic acid (SAHA) for 48 h.</abstracttext></p> <h4>MATERIALS AND METHODS:</h4> <p><abstracttext label="MATERIALS AND METHODS" nlmcategory="METHODS">Chromatin immunoprecipitation (ChIP) was used to observe HDACis (SAHA and NaBu) and HMTi (DZNep) impact on histones and more specifically on H3K27me3, H3K9ac and H3K4ac marks with Q-PCR analysis of BRCA1, SRC3 and P300 genes. Furthermore, the HDACi and HMTi effects on mRNA and protein expression of BRCA1, SRC3 and P300 genes were checked. In addition, statistical analyses were used.</abstracttext></p> <h4>RESULTS:</h4> <p><abstracttext label="RESULTS" nlmcategory="RESULTS">In the MCF7 luminal subtype with positive ER, H3k4ac was significantly increased on BRCA1 with SAHA. On the contrary, in the MDA-MB 231 breast cancer cell line, representative of mesenchymal-like subtype with negative estrogen receptor, HDACis had no effect. Also, DZNEP decreased significantly H3K27me3 on BRCA1 in MDA-MB 231. Besides, on SRC3, a significant increase for H3K4ac was obtained in MCF7 treated with SAHA. And DZNEP had no effect in MCF7. Also, in MDA-MB 231 treated with DZNEP, H3K27me3 significantly decreased on SRC3 while H3K4ac was significantly increased in MDA-MB-231 treated with SAHA or NaBu for P300.</abstracttext></p> <h4>CONCLUSION:</h4> <p><abstracttext label="CONCLUSION" nlmcategory="CONCLUSIONS">Luminal and mesenchymal-like breast cancer subtype cell lines seemed to act differently to HDACis (SAHA and NaBu) or HMTi (DZNEP) treatments.</abstracttext></p>', 'date' => '2016-07-01', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/27365379', 'doi' => '', 'modified' => '2016-07-12 12:50:21', 'created' => '2016-07-12 12:46:04', 'ProductsPublication' => array( 'id' => '1434', 'product_id' => '2517', 'publication_id' => '2980' ) ) $externalLink = ' <a href="http://www.ncbi.nlm.nih.gov/pubmed/27365379" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
Notice (8): Undefined variable: campaign_id [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL --><div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog"><?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => 'Human ChIP-seq grade c-fos promoter primer pair', 'meta_title' => 'Human ChIP-seq grade c-fos promoter primer pair', 'product' => array( 'Product' => array( 'id' => '2517', 'antibody_id' => null, 'name' => 'Human ChIP-seq grade c-fos promoter primer pair', 'description' => '<p><span>This primer pair specifically amplifies a genomic region containing the human c-fos promoter. The primers are thoroughly tested and optimized for routine SYBR® Green Real-Time qPCR assay following ChIP and for ChIP-sequencing library validation (e.g. before and after ChIP-seq library preparation).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011004-50', 'old_catalog_number' => 'pp-1004-050', 'sf_code' => 'C17011004-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-chip-seq-grade-c-fos-promoter-primer-pair-50-ul', 'meta_title' => 'Human ChIP-seq grade c-fos promoter primer pair', 'meta_keywords' => '', 'meta_description' => 'Human ChIP-seq grade c-fos promoter primer pair', 'modified' => '2016-10-21 08:17:25', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( [maximum depth reached] ) ), 'Group' => array( 'Group' => array( [maximum depth reached] ), 'Master' => array( [maximum depth reached] ), 'Product' => array( [maximum depth reached] ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ) ), 'meta_canonical' => 'https://www.diagenode.com/jp/p/human-chip-seq-grade-c-fos-promoter-primer-pair-50-ul' ) $language = 'jp' $meta_keywords = '' $meta_description = 'Human ChIP-seq grade c-fos promoter primer pair' $meta_title = 'Human ChIP-seq grade c-fos promoter primer pair' $product = array( 'Product' => array( 'id' => '2517', 'antibody_id' => null, 'name' => 'Human ChIP-seq grade c-fos promoter primer pair', 'description' => '<p><span>This primer pair specifically amplifies a genomic region containing the human c-fos promoter. The primers are thoroughly tested and optimized for routine SYBR® Green Real-Time qPCR assay following ChIP and for ChIP-sequencing library validation (e.g. before and after ChIP-seq library preparation).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011004-50', 'old_catalog_number' => 'pp-1004-050', 'sf_code' => 'C17011004-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-chip-seq-grade-c-fos-promoter-primer-pair-50-ul', 'meta_title' => 'Human ChIP-seq grade c-fos promoter primer pair', 'meta_keywords' => '', 'meta_description' => 'Human ChIP-seq grade c-fos promoter primer pair', 'modified' => '2016-10-21 08:17:25', 'created' => '2015-06-29 14:08:20', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array( (int) 0 => array( 'id' => '118', 'name' => 'C17011004', 'product_id' => '2517', 'modified' => '2016-02-19 21:27:26', 'created' => '2016-02-19 21:27:26' ) ), 'Group' => array( 'Group' => array( 'id' => '118', 'name' => 'C17011004', 'product_id' => '2517', 'modified' => '2016-02-19 21:27:26', 'created' => '2016-02-19 21:27:26' ), 'Master' => array( 'id' => '2517', 'antibody_id' => null, 'name' => 'Human ChIP-seq grade c-fos promoter primer pair', 'description' => '<p><span>This primer pair specifically amplifies a genomic region containing the human c-fos promoter. The primers are thoroughly tested and optimized for routine SYBR® Green Real-Time qPCR assay following ChIP and for ChIP-sequencing library validation (e.g. before and after ChIP-seq library preparation).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '50 µl', 'catalog_number' => 'C17011004-50', 'old_catalog_number' => 'pp-1004-050', 'sf_code' => 'C17011004-D001-000014', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '60', 'price_USD' => '35', 'price_GBP' => '60', 'price_JPY' => '9400', 'price_CNY' => '', 'price_AUD' => '88', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-chip-seq-grade-c-fos-promoter-primer-pair-50-ul', 'meta_title' => 'Human ChIP-seq grade c-fos promoter primer pair', 'meta_keywords' => '', 'meta_description' => 'Human ChIP-seq grade c-fos promoter primer pair', 'modified' => '2016-10-21 08:17:25', 'created' => '2015-06-29 14:08:20' ), 'Product' => array( (int) 0 => array( [maximum depth reached] ) ) ), 'Related' => array(), 'Application' => array(), 'Category' => array( (int) 0 => array( 'id' => '69', 'position' => '1', 'parent_id' => '35', 'name' => 'Human', 'description' => '<p>Primer pairs - Human</p>', 'no_promo' => false, 'in_menu' => false, 'online' => true, 'tabular' => true, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'primer-pairs-human', 'cookies_tag_id' => null, 'meta_keywords' => 'Primer pairs, human, ChIP-seq grade', 'meta_description' => 'Diagenode offers Primer pairs for Human ChIP-seq grade', 'meta_title' => 'Primer pairs - Human | Diagenode', 'modified' => '2016-10-21 08:00:50', 'created' => '2015-07-23 15:38:42', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '254', 'name' => 'Datasheet Human cfos promoter pp1004', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/primer_pairs/Datasheet_Human_cfos_promoter_pp1004.pdf', 'slug' => 'datasheet-human-cfos-promoter-pp1004', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array(), 'Image' => array(), 'Promotion' => array(), 'Protocol' => array(), 'Publication' => array( (int) 0 => array( 'id' => '2980', 'name' => 'Epigenetic Modifications with DZNep, NaBu and SAHA in Luminal and Mesenchymal-like Breast Cancer Subtype Cells', 'authors' => 'Dagdemir A et al.', 'description' => '<h4>BACKGROUND/AIM:</h4> <p><abstracttext label="BACKGROUND/AIM" nlmcategory="OBJECTIVE">Numerous studies have shown that breast cancer and epigenetic mechanisms have a very powerful interactive relation. The MCF7 cell line, representative of luminal subtype and the MDA-MB 231 cell line representative of mesenchymal-like subtype were treated respectively with a Histone Methyl Transferase Inhibitors (HMTi), 3-Deazaneplanocin hydrochloride (DZNep), two histone deacetylase inhibitors (HDACi), sodium butyrate (NaBu), and suberoylanilide hydroxamic acid (SAHA) for 48 h.</abstracttext></p> <h4>MATERIALS AND METHODS:</h4> <p><abstracttext label="MATERIALS AND METHODS" nlmcategory="METHODS">Chromatin immunoprecipitation (ChIP) was used to observe HDACis (SAHA and NaBu) and HMTi (DZNep) impact on histones and more specifically on H3K27me3, H3K9ac and H3K4ac marks with Q-PCR analysis of BRCA1, SRC3 and P300 genes. Furthermore, the HDACi and HMTi effects on mRNA and protein expression of BRCA1, SRC3 and P300 genes were checked. In addition, statistical analyses were used.</abstracttext></p> <h4>RESULTS:</h4> <p><abstracttext label="RESULTS" nlmcategory="RESULTS">In the MCF7 luminal subtype with positive ER, H3k4ac was significantly increased on BRCA1 with SAHA. On the contrary, in the MDA-MB 231 breast cancer cell line, representative of mesenchymal-like subtype with negative estrogen receptor, HDACis had no effect. Also, DZNEP decreased significantly H3K27me3 on BRCA1 in MDA-MB 231. Besides, on SRC3, a significant increase for H3K4ac was obtained in MCF7 treated with SAHA. And DZNEP had no effect in MCF7. Also, in MDA-MB 231 treated with DZNEP, H3K27me3 significantly decreased on SRC3 while H3K4ac was significantly increased in MDA-MB-231 treated with SAHA or NaBu for P300.</abstracttext></p> <h4>CONCLUSION:</h4> <p><abstracttext label="CONCLUSION" nlmcategory="CONCLUSIONS">Luminal and mesenchymal-like breast cancer subtype cell lines seemed to act differently to HDACis (SAHA and NaBu) or HMTi (DZNEP) treatments.</abstracttext></p>', 'date' => '2016-07-01', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/27365379', 'doi' => '', 'modified' => '2016-07-12 12:50:21', 'created' => '2016-07-12 12:46:04', 'ProductsPublication' => array( [maximum depth reached] ) ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array( (int) 0 => array( 'id' => '270', 'name' => 'c-fos Promoter Primer Pair SDS GB en', 'language' => 'en', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-GB-en-GHS_1_0.pdf', 'countries' => 'GB', 'modified' => '2020-06-09 10:33:07', 'created' => '2020-06-09 10:33:07', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '272', 'name' => 'c-fos Promoter Primer Pair SDS US en', 'language' => 'en', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-US-en-GHS_1_0.pdf', 'countries' => 'US', 'modified' => '2020-06-09 10:34:22', 'created' => '2020-06-09 10:34:22', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '267', 'name' => 'c-fos Promoter Primer Pair SDS DE de', 'language' => 'de', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-DE-de-GHS_1_0.pdf', 'countries' => 'DE', 'modified' => '2020-06-09 10:27:14', 'created' => '2020-06-09 10:27:14', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '271', 'name' => 'c-fos Promoter Primer Pair SDS JP ja', 'language' => 'ja', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-JP-ja-GHS_2_0.pdf', 'countries' => 'JP', 'modified' => '2020-06-09 10:33:45', 'created' => '2020-06-09 10:33:45', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '266', 'name' => 'c-fos Promoter Primer Pair SDS BE nl', 'language' => 'nl', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-BE-nl-GHS_1_0.pdf', 'countries' => 'BE', 'modified' => '2020-06-09 10:25:59', 'created' => '2020-06-09 10:25:59', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '265', 'name' => 'c-fos Promoter Primer Pair SDS BE fr', 'language' => 'fr', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-BE-fr-GHS_1_0.pdf', 'countries' => 'BE', 'modified' => '2020-06-09 10:24:34', 'created' => '2020-06-09 10:24:34', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '269', 'name' => 'c-fos Promoter Primer Pair SDS FR fr', 'language' => 'fr', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-FR-fr-GHS_1_0.pdf', 'countries' => 'FR', 'modified' => '2020-06-09 10:29:06', 'created' => '2020-06-09 10:29:06', 'ProductsSafetySheet' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '268', 'name' => 'c-fos Promoter Primer Pair SDS ES es', 'language' => 'es', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-ES-es-GHS_1_0.pdf', 'countries' => 'ES', 'modified' => '2020-06-09 10:28:17', 'created' => '2020-06-09 10:28:17', 'ProductsSafetySheet' => array( [maximum depth reached] ) ) ) ) $meta_canonical = 'https://www.diagenode.com/jp/p/human-chip-seq-grade-c-fos-promoter-primer-pair-50-ul' $country = 'US' $countries_allowed = array( (int) 0 => 'CA', (int) 1 => 'US', (int) 2 => 'IE', (int) 3 => 'GB', (int) 4 => 'DK', (int) 5 => 'NO', (int) 6 => 'SE', (int) 7 => 'FI', (int) 8 => 'NL', (int) 9 => 'BE', (int) 10 => 'LU', (int) 11 => 'FR', (int) 12 => 'DE', (int) 13 => 'CH', (int) 14 => 'AT', (int) 15 => 'ES', (int) 16 => 'IT', (int) 17 => 'PT' ) $outsource = false $other_formats = array( (int) 0 => array( 'id' => '2518', 'antibody_id' => null, 'name' => 'Human ChIP-seq grade c-fos promoter primer pair', 'description' => '<p><span>This primer pair specifically amplifies a genomic region containing the human c-fos promoter. The primers are thoroughly tested and optimized for routine SYBR® Green Real-Time qPCR assay following ChIP and for ChIP-sequencing library validation (e.g. before and after ChIP-seq library preparation).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011004-500', 'old_catalog_number' => 'pp-1004-500', 'sf_code' => 'C17011004-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-chip-seq-grade-c-fos-promoter-primer-pair-500-ul', 'meta_title' => 'Human ChIP-seq grade c-fos promoter primer pair', 'meta_keywords' => '', 'meta_description' => 'Human ChIP-seq grade c-fos promoter primer pair', 'modified' => '2016-02-19 21:27:54', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '129', 'product_id' => '2518', 'group_id' => '118' ) ) ) $pro = array( 'id' => '2518', 'antibody_id' => null, 'name' => 'Human ChIP-seq grade c-fos promoter primer pair', 'description' => '<p><span>This primer pair specifically amplifies a genomic region containing the human c-fos promoter. The primers are thoroughly tested and optimized for routine SYBR® Green Real-Time qPCR assay following ChIP and for ChIP-sequencing library validation (e.g. before and after ChIP-seq library preparation).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011004-500', 'old_catalog_number' => 'pp-1004-500', 'sf_code' => 'C17011004-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-chip-seq-grade-c-fos-promoter-primer-pair-500-ul', 'meta_title' => 'Human ChIP-seq grade c-fos promoter primer pair', 'meta_keywords' => '', 'meta_description' => 'Human ChIP-seq grade c-fos promoter primer pair', 'modified' => '2016-02-19 21:27:54', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '129', 'product_id' => '2518', 'group_id' => '118' ) ) $edit = '' $testimonials = '' $featured_testimonials = '' $related_products = '' $rrbs_service = array( (int) 0 => (int) 1894, (int) 1 => (int) 1895 ) $chipseq_service = array( (int) 0 => (int) 2683, (int) 1 => (int) 1835, (int) 2 => (int) 1836, (int) 3 => (int) 2684, (int) 4 => (int) 1838, (int) 5 => (int) 1839, (int) 6 => (int) 1856 ) $labelize = object(Closure) { } $old_catalog_number = ' <span style="color:#CCC">(pp-1004-500)</span>' $country_code = 'US' $other_format = array( 'id' => '2518', 'antibody_id' => null, 'name' => 'Human ChIP-seq grade c-fos promoter primer pair', 'description' => '<p><span>This primer pair specifically amplifies a genomic region containing the human c-fos promoter. The primers are thoroughly tested and optimized for routine SYBR® Green Real-Time qPCR assay following ChIP and for ChIP-sequencing library validation (e.g. before and after ChIP-seq library preparation).</span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '500 µl', 'catalog_number' => 'C17011004-500', 'old_catalog_number' => 'pp-1004-500', 'sf_code' => 'C17011004-D001-000015', 'type' => 'FRE', 'search_order' => '04-undefined', 'price_EUR' => '115', 'price_USD' => '100', 'price_GBP' => '105', 'price_JPY' => '18015', 'price_CNY' => '', 'price_AUD' => '250', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => false, 'last_datasheet_update' => '0000-00-00', 'slug' => 'human-chip-seq-grade-c-fos-promoter-primer-pair-500-ul', 'meta_title' => 'Human ChIP-seq grade c-fos promoter primer pair', 'meta_keywords' => '', 'meta_description' => 'Human ChIP-seq grade c-fos promoter primer pair', 'modified' => '2016-02-19 21:27:54', 'created' => '2015-06-29 14:08:20', 'ProductsGroup' => array( 'id' => '129', 'product_id' => '2518', 'group_id' => '118' ) ) $label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>' $document = array( 'id' => '254', 'name' => 'Datasheet Human cfos promoter pp1004', 'description' => 'Datasheet description', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/reagents/primer_pairs/Datasheet_Human_cfos_promoter_pp1004.pdf', 'slug' => 'datasheet-human-cfos-promoter-pp1004', 'meta_keywords' => null, 'meta_description' => null, 'modified' => '2015-07-07 11:47:43', 'created' => '2015-07-07 11:47:43', 'ProductsDocument' => array( 'id' => '586', 'product_id' => '2517', 'document_id' => '254' ) ) $sds = array( 'id' => '268', 'name' => 'c-fos Promoter Primer Pair SDS ES es', 'language' => 'es', 'url' => 'files/SDS/c-fos_promoter/SDS-C17011004-c-fos_promoter_primer_pair-ES-es-GHS_1_0.pdf', 'countries' => 'ES', 'modified' => '2020-06-09 10:28:17', 'created' => '2020-06-09 10:28:17', 'ProductsSafetySheet' => array( 'id' => '502', 'product_id' => '2517', 'safety_sheet_id' => '268' ) ) $publication = array( 'id' => '2980', 'name' => 'Epigenetic Modifications with DZNep, NaBu and SAHA in Luminal and Mesenchymal-like Breast Cancer Subtype Cells', 'authors' => 'Dagdemir A et al.', 'description' => '<h4>BACKGROUND/AIM:</h4> <p><abstracttext label="BACKGROUND/AIM" nlmcategory="OBJECTIVE">Numerous studies have shown that breast cancer and epigenetic mechanisms have a very powerful interactive relation. The MCF7 cell line, representative of luminal subtype and the MDA-MB 231 cell line representative of mesenchymal-like subtype were treated respectively with a Histone Methyl Transferase Inhibitors (HMTi), 3-Deazaneplanocin hydrochloride (DZNep), two histone deacetylase inhibitors (HDACi), sodium butyrate (NaBu), and suberoylanilide hydroxamic acid (SAHA) for 48 h.</abstracttext></p> <h4>MATERIALS AND METHODS:</h4> <p><abstracttext label="MATERIALS AND METHODS" nlmcategory="METHODS">Chromatin immunoprecipitation (ChIP) was used to observe HDACis (SAHA and NaBu) and HMTi (DZNep) impact on histones and more specifically on H3K27me3, H3K9ac and H3K4ac marks with Q-PCR analysis of BRCA1, SRC3 and P300 genes. Furthermore, the HDACi and HMTi effects on mRNA and protein expression of BRCA1, SRC3 and P300 genes were checked. In addition, statistical analyses were used.</abstracttext></p> <h4>RESULTS:</h4> <p><abstracttext label="RESULTS" nlmcategory="RESULTS">In the MCF7 luminal subtype with positive ER, H3k4ac was significantly increased on BRCA1 with SAHA. On the contrary, in the MDA-MB 231 breast cancer cell line, representative of mesenchymal-like subtype with negative estrogen receptor, HDACis had no effect. Also, DZNEP decreased significantly H3K27me3 on BRCA1 in MDA-MB 231. Besides, on SRC3, a significant increase for H3K4ac was obtained in MCF7 treated with SAHA. And DZNEP had no effect in MCF7. Also, in MDA-MB 231 treated with DZNEP, H3K27me3 significantly decreased on SRC3 while H3K4ac was significantly increased in MDA-MB-231 treated with SAHA or NaBu for P300.</abstracttext></p> <h4>CONCLUSION:</h4> <p><abstracttext label="CONCLUSION" nlmcategory="CONCLUSIONS">Luminal and mesenchymal-like breast cancer subtype cell lines seemed to act differently to HDACis (SAHA and NaBu) or HMTi (DZNEP) treatments.</abstracttext></p>', 'date' => '2016-07-01', 'pmid' => 'http://www.ncbi.nlm.nih.gov/pubmed/27365379', 'doi' => '', 'modified' => '2016-07-12 12:50:21', 'created' => '2016-07-12 12:46:04', 'ProductsPublication' => array( 'id' => '1434', 'product_id' => '2517', 'publication_id' => '2980' ) ) $externalLink = ' <a href="http://www.ncbi.nlm.nih.gov/pubmed/27365379" target="_blank"><i class="fa fa-external-link"></i></a>'include - APP/View/Products/view.ctp, line 755 View::_evaluate() - CORE/Cake/View/View.php, line 971 View::_render() - CORE/Cake/View/View.php, line 933 View::render() - CORE/Cake/View/View.php, line 473 Controller::render() - CORE/Cake/Controller/Controller.php, line 963 ProductsController::slug() - APP/Controller/ProductsController.php, line 1052 ReflectionMethod::invokeArgs() - [internal], line ?? Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491 Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193 Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167 [main] - APP/webroot/index.php, line 118
To ensure you see the information most relevant to you, please select your country. Please note that your browser will need to be configured to accept cookies.
Diagenode will process your personal data in strict accordance with its privacy policy. This will include sending you updates about us, our products, and resources we think would be of interest to you.