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JMJD2a polyclonal antibody

CUT&Tag grade Abs

ChIP-seq grade Abs

ChIP grade Abs

Negative IgG control

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Catalog Number

Format

Price

C15410332

100 μl

$460.00
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Other format

C15410332-20

20 μl

Polyclonal antibody raised in rabbit against human JMJD2a (Jumonji domain containing 2a), using a synthetic peptide containing a sequence from the C-terminal part of the protein.

1Manufactured by Bethyl Laboratories, Inc., Texas, USA

Lot	A300-861A2
Concentration	100 μl
Species reactivity	Human, mouse
Type	Polyclonal ChIP grade, ChIP-seq grade
Purity	Affinity purified
Host	Rabbit
Storage Conditions	Store at 4°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Precautions	This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Applications	Suggested dilution	References
ChIP / ChIP-seq ^*	5 μg/ChIP	Fig 1, 2
Western Blotting	1:500	Fig 3
IP	3 μg per IP	Fig 4

^* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

Validation data

Figure 1. ChIP results obtained with the Diagenode antibody directed against JMJD2a
ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

A.
B.
C.
D.

Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JMJD2a
ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Figure 3. Western blot analysis using the Diagenode antibody directed against JMJD2a
Whole cell extracts from HeLa (lane 1) and mouse NIH3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. C15410332) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against JMJD2a
Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 μg of the Diagenode antibody against JMJD2a (Cat. No. C15410332, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JMJD2a protein was detected by western blot with the JMJD2a antibody diluted 1:200.

Manufactured by Bethyl Laboratories, Inc., Texas, USA
Target Description
JMJD2a (UniProtKB/Swiss-Prot entry O75164), belongs to the JMJD2 family of histone demethylases which play an important role in the establishment of the histone code. JMJD2a specifically demethylates the trimethylated K9 and K36 of histone H3, thereby converting these lysines to the dimethylated form. It has no activity towards H3K4, H3K27 and H4K20, or to the mono- and dimethylated H3K9 and H3K36. JMJD2a plays a role in the transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.

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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Whole cell extracts from HeLa (lane 1) and mouse NIH3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. C15410332) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15410332-ip.png" alt="JMJD2a Antibody validated in Immunoprecipitation " width="137" height="214" caption="false" /></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 &mu;g of the Diagenode antibody against JMJD2a (Cat. No. C15410332, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JMJD2a protein was detected by western blot with the JMJD2a antibody diluted 1:200. </small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Whole cell extracts from HeLa (lane 1) and mouse NIH3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. C15410332) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 &mu;g of the Diagenode antibody against JMJD2a (Cat. No. C15410332, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JMJD2a protein was detected by western blot with the JMJD2a antibody diluted 1:200. </small></p>
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<tbody>
<tr>
<td>ChIP / ChIP-seq <sup>*</sup></td>
<td>5 &mu;g/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td><span>Western Blotting</span></td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>IP</td>
<td><span>3 &mu;g per IP</span></td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p></p>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 &mu;g per IP.</small></p>',
		'storage_conditions' => 'Store at 4°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
		'storage_buffer' => '',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
		'uniprot_acc' => '',
		'slug' => 'jmjd2a-polyclonal-antibody',
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		'modified' => '2022-03-25 11:39:48',
		'created' => '2016-12-12 16:36:38',
		'select_label' => '498 - JMJD2a polyclonal antibody (A300-861A2 - 100 μl - Human, mouse - Affinity purified - Rabbit)'
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			'name' => 'JMJD2a Antibody',
			'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>JMJD2a (Jumonji domain containing 2a)</strong>, using a synthetic peptide containing a sequence from the C-terminal part of the protein.</p>
<p></p>',
			'label1' => 'Validation data',
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410332-chip.png" alt="JMJD2a Antibody ChIP Grade" caption="false" width="288" height="218" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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</div>
<div class="row">
<div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-a.jpg" alt="JMJD2a Antibody ChIP-seq Grade" caption="false" width="922" height="109" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-b.jpg" alt="JMJD2a Antibody for ChIP-seq assay" caption="false" width="922" height="181" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-c.jpg" alt="JMJD2a Antibody validated in ChIP-seq" caption="false" width="922" height="139" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-d.jpg" alt="JMJD2a Antibody for ChIP-seq" caption="false" width="922" height="122" /></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15410332-wb.png" alt="JMJD2a Antibody validated in Western Blot" width="143" height="248" caption="false" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Whole cell extracts from HeLa (lane 1) and mouse NIH3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. C15410332) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410332-ip.png" alt="JMJD2a Antibody validated in Immunoprecipitation " width="137" height="214" caption="false" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 &mu;g of the Diagenode antibody against JMJD2a (Cat. No. C15410332, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JMJD2a protein was detected by western blot with the JMJD2a antibody diluted 1:200. </small></p>
</div>
&nbsp;&nbsp; <small>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></div>',
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			'info2' => '<p>JMJD2a (UniProtKB/Swiss-Prot entry O75164), belongs to the JMJD2 family of histone demethylases which play an important role in the establishment of the histone code. JMJD2a specifically demethylates the trimethylated K9 and K36 of histone H3, thereby converting these lysines to the dimethylated form. It has no activity towards H3K4, H3K27 and H4K20, or to the mono- and dimethylated H3K9 and H3K36. JMJD2a plays a role in the transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.</p>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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<li>Highly sensitive and specific</li>
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<li>Batch-specific data is available on the website</li>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode&rsquo;s highly validated antibodies:</p>
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<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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			'description' => '<p>Polyclonal antibody raised in rabbit against human JMJD2a (Jumonji domain containing 2a), using a synthetic peptide containing a sequence from the C-terminal part of the protein.</p>
<p><small>1Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>',
			'label1' => 'Validation data',
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410332-chip.png" alt="JMJD2a Antibody ChIP Grade" caption="false" width="288" height="218" /></p>
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<div class="small-8 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-a.jpg" alt="JMJD2a Antibody ChIP-seq Grade" caption="false" width="922" height="109" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-b.jpg" alt="JMJD2a Antibody for ChIP-seq assay" caption="false" width="922" height="181" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-c.jpg" alt="JMJD2a Antibody validated in ChIP-seq" caption="false" width="922" height="139" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-d.jpg" alt="JMJD2a Antibody for ChIP-seq" caption="false" width="922" height="122" /></div>
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<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15410332-wb.png" alt="JMJD2a Antibody validated in Western Blot" width="143" height="248" caption="false" /></p>
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<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Whole cell extracts from HeLa (lane 1) and mouse NIH3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. C15410332) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410332-ip.png" alt="JMJD2a Antibody validated in Immunoprecipitation " width="137" height="214" caption="false" /></p>
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<div class="small-9 columns">
<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 &mu;g of the Diagenode antibody against JMJD2a (Cat. No. C15410332, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JMJD2a protein was detected by western blot with the JMJD2a antibody diluted 1:200. </small></p>
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<p><small>1Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>',
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Whole cell extracts from HeLa (lane 1) and mouse NIH3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. C15410332) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 &mu;g of the Diagenode antibody against JMJD2a (Cat. No. C15410332, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JMJD2a protein was detected by western blot with the JMJD2a antibody diluted 1:200. </small></p>
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&nbsp;&nbsp; <small>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></div>',
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Whole cell extracts from HeLa (lane 1) and mouse NIH3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. C15410332) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 &mu;g of the Diagenode antibody against JMJD2a (Cat. No. C15410332, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JMJD2a protein was detected by western blot with the JMJD2a antibody diluted 1:200. </small></p>
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&nbsp;&nbsp; <small>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></div>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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			'description' => '<p>Polyclonal antibody raised in rabbit against human JMJD2a (Jumonji domain containing 2a), using a synthetic peptide containing a sequence from the C-terminal part of the protein.</p>
<p><small>1Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15410332-chip.png" alt="JMJD2a Antibody ChIP Grade" caption="false" width="288" height="218" /></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-a.jpg" alt="JMJD2a Antibody ChIP-seq Grade" caption="false" width="922" height="109" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-b.jpg" alt="JMJD2a Antibody for ChIP-seq assay" caption="false" width="922" height="181" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-c.jpg" alt="JMJD2a Antibody validated in ChIP-seq" caption="false" width="922" height="139" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-d.jpg" alt="JMJD2a Antibody for ChIP-seq" caption="false" width="922" height="122" /></div>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Whole cell extracts from HeLa (lane 1) and mouse NIH3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. C15410332) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 &mu;g of the Diagenode antibody against JMJD2a (Cat. No. C15410332, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JMJD2a protein was detected by western blot with the JMJD2a antibody diluted 1:200. </small></p>
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			'type' => 'Polyclonal <strong>ChIP grade, ChIP-seq grade</strong>',
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<th>References</th>
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<td>ChIP / ChIP-seq <sup>*</sup></td>
<td>5 &mu;g/ChIP</td>
<td>Fig 1, 2</td>
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<tr>
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<td>1:500</td>
<td>Fig 3</td>
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410332-chip.png" alt="JMJD2a Antibody ChIP Grade" caption="false" width="288" height="218" /></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-a.jpg" alt="JMJD2a Antibody ChIP-seq Grade" caption="false" width="922" height="109" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-b.jpg" alt="JMJD2a Antibody for ChIP-seq assay" caption="false" width="922" height="181" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-c.jpg" alt="JMJD2a Antibody validated in ChIP-seq" caption="false" width="922" height="139" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-d.jpg" alt="JMJD2a Antibody for ChIP-seq" caption="false" width="922" height="122" /></div>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Whole cell extracts from HeLa (lane 1) and mouse NIH3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. C15410332) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 &mu;g of the Diagenode antibody against JMJD2a (Cat. No. C15410332, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JMJD2a protein was detected by western blot with the JMJD2a antibody diluted 1:200. </small></p>
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&nbsp;&nbsp; <small>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></div>',
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		'name' => 'JMJD2a polyclonal antibody',
		'description' => 'JMJD2a (UniProtKB/Swiss-Prot entry O75164), belongs to the JMJD2 family of histone demethylases which play an important role in the establishment of the histone code. JMJD2a specifically demethylates the trimethylated K9 and K36 of histone H3, thereby converting these lysines to the dimethylated form. It has no activity towards H3K4, H3K27 and H4K20, or to the mono- and dimethylated H3K9 and H3K36. JMJD2a plays a role in the transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.',
		'clonality' => '',
		'isotype' => '',
		'lot' => 'A300-861A2',
		'concentration' => '100 μl',
		'reactivity' => 'Human, mouse',
		'type' => 'Polyclonal <strong>ChIP grade, ChIP-seq grade</strong>',
		'purity' => 'Affinity purified',
		'classification' => 'Classic',
		'application_table' => '<table>
<thead>
<tr>
<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
</thead>
<tbody>
<tr>
<td>ChIP / ChIP-seq <sup>*</sup></td>
<td>5 &mu;g/ChIP</td>
<td>Fig 1, 2</td>
</tr>
<tr>
<td><span>Western Blotting</span></td>
<td>1:500</td>
<td>Fig 3</td>
</tr>
<tr>
<td>IP</td>
<td><span>3 &mu;g per IP</span></td>
<td>Fig 4</td>
</tr>
</tbody>
</table>
<p></p>
<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 &mu;g per IP.</small></p>',
		'storage_conditions' => 'Store at 4°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.',
		'storage_buffer' => '',
		'precautions' => 'This product is for research use only. Not for use in diagnostic or therapeutic procedures.',
		'uniprot_acc' => '',
		'slug' => 'jmjd2a-polyclonal-antibody',
		'meta_keywords' => '',
		'meta_description' => '',
		'modified' => '2022-03-25 11:39:48',
		'created' => '2016-12-12 16:36:38',
		'select_label' => '498 - JMJD2a polyclonal antibody (A300-861A2 - 100 μl - Human, mouse - Affinity purified - Rabbit)'
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			'antibody_id' => '498',
			'name' => 'JMJD2a Antibody',
			'description' => '<p>Polyclonal antibody raised in rabbit against human <strong>JMJD2a (Jumonji domain containing 2a)</strong>, using a synthetic peptide containing a sequence from the C-terminal part of the protein.</p>
<p></p>',
			'label1' => 'Validation data',
			'info1' => '<div class="row">
<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410332-chip.png" alt="JMJD2a Antibody ChIP Grade" caption="false" width="288" height="218" /></p>
</div>
<div class="small-8 columns">
<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
</div>
</div>
<div class="row">
<div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-a.jpg" alt="JMJD2a Antibody ChIP-seq Grade" caption="false" width="922" height="109" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-b.jpg" alt="JMJD2a Antibody for ChIP-seq assay" caption="false" width="922" height="181" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-c.jpg" alt="JMJD2a Antibody validated in ChIP-seq" caption="false" width="922" height="139" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-d.jpg" alt="JMJD2a Antibody for ChIP-seq" caption="false" width="922" height="122" /></div>
</div>
<div class="row">
<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
</div>
</div>
<div class="spacer"></div>
<div class="spacer"></div>
<div class="spacer"></div>
<div class="spacer"></div>
<div class="spacer"></div>
<div class="spacer"></div>
<div class="spacer"></div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410332-wb.png" alt="JMJD2a Antibody validated in Western Blot" width="143" height="248" caption="false" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Whole cell extracts from HeLa (lane 1) and mouse NIH3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. C15410332) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
</div>
</div>
<div class="row">
<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410332-ip.png" alt="JMJD2a Antibody validated in Immunoprecipitation " width="137" height="214" caption="false" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 &mu;g of the Diagenode antibody against JMJD2a (Cat. No. C15410332, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JMJD2a protein was detected by western blot with the JMJD2a antibody diluted 1:200. </small></p>
</div>
&nbsp;&nbsp; <small>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></div>',
			'label2' => 'Target Description',
			'info2' => '<p>JMJD2a (UniProtKB/Swiss-Prot entry O75164), belongs to the JMJD2 family of histone demethylases which play an important role in the establishment of the histone code. JMJD2a specifically demethylates the trimethylated K9 and K36 of histone H3, thereby converting these lysines to the dimethylated form. It has no activity towards H3K4, H3K27 and H4K20, or to the mono- and dimethylated H3K9 and H3K36. JMJD2a plays a role in the transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.</p>',
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			'format' => '100 μl',
			'catalog_number' => 'C15410332',
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			'sf_code' => 'C15410332-D001-001161',
			'type' => 'FRE',
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			'price_EUR' => '435',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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			'description' => '<p><span style="font-weight: 400;">All Diagenode&rsquo;s antibodies are listed below. Please, use our Quick search field to find the antibody of interest by target name, application, purity.</span></p>
<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
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<div class="small-12 columns"><center></center>
<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
<p></p>
</div>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
<div class="row">
<div class="small-12 medium-6 large-6 columns"><img src="https://www.diagenode.com/img/product/antibodies/C15410003-fig1-ChIP.jpg" alt="" width="400" height="315" />&nbsp;</div>
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<p></p>
<p></p>
<p></p>
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<p>Our aim at Diagenode is to offer the largest collection of highly specific <strong>ChIP-grade antibodies</strong>. We add new antibodies monthly. Find your ChIP-grade antibody in the list below and check more information about tested applications, extensive validation data, and product information.</p>',
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			'meta_description' => 'Diagenode Offers Extensively Validated ChIP-Grade Antibodies, Confirmed for their Specificity, and high level of Performance in Chromatin Immunoprecipitation ChIP',
			'meta_title' => 'Chromatin immunoprecipitation ChIP-grade antibodies | Diagenode',
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			'description' => '<p><b>Unparalleled ChIP-Seq results with the most rigorously validated antibodies</b></p>
<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
<div class="small-12 medium-3 large-3 columns">
<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p>Diagenode&rsquo;s highly validated antibodies:</p>
<ul>
<li>Highly sensitive and specific</li>
<li>Cost-effective (requires less antibody per reaction)</li>
<li>Batch-specific data is available on the website</li>
<li>Expert technical support</li>
<li>Sample sizes available</li>
<li>100% satisfaction guarantee</li>
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			'name' => 'JMJD2a polyclonal antibody',
			'description' => '<p>Polyclonal antibody raised in rabbit against human JMJD2a (Jumonji domain containing 2a), using a synthetic peptide containing a sequence from the C-terminal part of the protein.</p>',
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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<p><small>1Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>',
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<div class="small-4 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410332-chip.png" alt="JMJD2a Antibody ChIP Grade" caption="false" width="288" height="218" /></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-a.jpg" alt="JMJD2a Antibody ChIP-seq Grade" caption="false" width="922" height="109" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-b.jpg" alt="JMJD2a Antibody for ChIP-seq assay" caption="false" width="922" height="181" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-c.jpg" alt="JMJD2a Antibody validated in ChIP-seq" caption="false" width="922" height="139" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-d.jpg" alt="JMJD2a Antibody for ChIP-seq" caption="false" width="922" height="122" /></div>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Whole cell extracts from HeLa (lane 1) and mouse NIH3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. C15410332) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15410332-ip.png" alt="JMJD2a Antibody validated in Immunoprecipitation " width="137" height="214" caption="false" /></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 &mu;g of the Diagenode antibody against JMJD2a (Cat. No. C15410332, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JMJD2a protein was detected by western blot with the JMJD2a antibody diluted 1:200. </small></p>
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<td>ChIP / ChIP-seq <sup>*</sup></td>
<td>5 &mu;g/ChIP</td>
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		'description' => '<p>Polyclonal antibody raised in rabbit against human JMJD2a (Jumonji domain containing 2a), using a synthetic peptide containing a sequence from the C-terminal part of the protein.</p>
<p><small>1Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></p>',
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Whole cell extracts from HeLa (lane 1) and mouse NIH3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. C15410332) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 &mu;g of the Diagenode antibody against JMJD2a (Cat. No. C15410332, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JMJD2a protein was detected by western blot with the JMJD2a antibody diluted 1:200. </small></p>
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&nbsp;&nbsp; <small>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></div>',
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<td>5 &mu;g/ChIP</td>
<td>Fig 1, 2</td>
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<td>1:500</td>
<td>Fig 3</td>
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<td><span>3 &mu;g per IP</span></td>
<td>Fig 4</td>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 &mu;g per IP.</small></p>',
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-a.jpg" alt="JMJD2a Antibody ChIP-seq Grade" caption="false" width="922" height="109" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-b.jpg" alt="JMJD2a Antibody for ChIP-seq assay" caption="false" width="922" height="181" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-c.jpg" alt="JMJD2a Antibody validated in ChIP-seq" caption="false" width="922" height="139" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-d.jpg" alt="JMJD2a Antibody for ChIP-seq" caption="false" width="922" height="122" /></div>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the &ldquo;iDeal ChIP-seq&rdquo; kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 &mu;g of antibody per ChIP experiment was analyzed. IgG (2 &mu;g/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Whole cell extracts from HeLa (lane 1) and mouse NIH3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. C15410332) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 &mu;g of the Diagenode antibody against JMJD2a (Cat. No. C15410332, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JMJD2a protein was detected by western blot with the JMJD2a antibody diluted 1:200. </small></p>
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&nbsp;&nbsp; <small>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></div>',
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
<p><em></em>Check our selection of antibodies validated in Western blot.</p>',
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<p><span style="font-weight: 400;">Diagenode&rsquo;s highly validated antibodies:</span></p>
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<p><br />Chromatin immunoprecipitation (<b>ChIP</b>) is a technique to study the associations of proteins with the specific genomic regions in intact cells. One of the most important steps of this protocol is the immunoprecipitation of targeted protein using the antibody specifically recognizing it. The quality of antibodies used in ChIP is essential for the success of the experiment. Diagenode offers extensively validated ChIP-grade antibodies, confirmed for their specificity, and high level of performance in ChIP. Each batch is validated, and batch-specific data are available on the website.</p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>).&nbsp;</p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 &micro;g of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 &micro;g of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118

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JMJD2a polyclonal antibody DATASHEET Polyclonal antibody raised in rabbit against human JMJD2a (Jumonji domain containing 2a), using a...	Download
Antibodies you can trust POSTER Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...	Download
Epigenetic Antibodies Brochure BROCHURE More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...	Download

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