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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Whole cell extracts from HeLa (lane 1) and mouse NIH3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. C15410332) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 μg of the Diagenode antibody against JMJD2a (Cat. No. C15410332, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JMJD2a protein was detected by western blot with the JMJD2a antibody diluted 1:200. </small></p>
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<p></p>',
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Whole cell extracts from HeLa (lane 1) and mouse NIH3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. C15410332) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 μg of the Diagenode antibody against JMJD2a (Cat. No. C15410332, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JMJD2a protein was detected by western blot with the JMJD2a antibody diluted 1:200. </small></p>
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<small>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></div>',
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<td>Fig 1, 2</td>
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<td><span>Western Blotting</span></td>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-a.jpg" alt="JMJD2a Antibody ChIP-seq Grade" caption="false" width="922" height="109" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-b.jpg" alt="JMJD2a Antibody for ChIP-seq assay" caption="false" width="922" height="181" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-c.jpg" alt="JMJD2a Antibody validated in ChIP-seq" caption="false" width="922" height="139" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-d.jpg" alt="JMJD2a Antibody for ChIP-seq" caption="false" width="922" height="122" /></div>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15410332-wb.png" alt="JMJD2a Antibody validated in Western Blot" width="143" height="248" caption="false" /></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Whole cell extracts from HeLa (lane 1) and mouse NIH3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. C15410332) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15410332-ip.png" alt="JMJD2a Antibody validated in Immunoprecipitation " width="137" height="214" caption="false" /></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 μg of the Diagenode antibody against JMJD2a (Cat. No. C15410332, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JMJD2a protein was detected by western blot with the JMJD2a antibody diluted 1:200. </small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Whole cell extracts from HeLa (lane 1) and mouse NIH3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. C15410332) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 μg of the Diagenode antibody against JMJD2a (Cat. No. C15410332, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JMJD2a protein was detected by western blot with the JMJD2a antibody diluted 1:200. </small></p>
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<td>ChIP / ChIP-seq <sup>*</sup></td>
<td>5 μg/ChIP</td>
<td>Fig 1, 2</td>
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<tr>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>',
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Whole cell extracts from HeLa (lane 1) and mouse NIH3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. C15410332) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 μg of the Diagenode antibody against JMJD2a (Cat. No. C15410332, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JMJD2a protein was detected by western blot with the JMJD2a antibody diluted 1:200. </small></p>
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'application_table' => '<table>
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<th>Applications</th>
<th>Suggested dilution</th>
<th>References</th>
</tr>
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<td>ChIP / ChIP-seq <sup>*</sup></td>
<td>5 μg/ChIP</td>
<td>Fig 1, 2</td>
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<tr>
<td><span>Western Blotting</span></td>
<td>1:500</td>
<td>Fig 3</td>
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<td><span>3 μg per IP</span></td>
<td>Fig 4</td>
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<p><small><sup>*</sup> Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.</small></p>',
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15410332-chip.png" alt="JMJD2a Antibody ChIP Grade" caption="false" width="288" height="218" /></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-a.jpg" alt="JMJD2a Antibody ChIP-seq Grade" caption="false" width="922" height="109" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-b.jpg" alt="JMJD2a Antibody for ChIP-seq assay" caption="false" width="922" height="181" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-c.jpg" alt="JMJD2a Antibody validated in ChIP-seq" caption="false" width="922" height="139" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-d.jpg" alt="JMJD2a Antibody for ChIP-seq" caption="false" width="922" height="122" /></div>
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<div class="small-12 columns">
<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15410332-wb.png" alt="JMJD2a Antibody validated in Western Blot" width="143" height="248" caption="false" /></p>
</div>
<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Whole cell extracts from HeLa (lane 1) and mouse NIH3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. C15410332) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<div class="small-3 columns">
<p><img src="https://www.diagenode.com/img/product/antibodies/c15410332-ip.png" alt="JMJD2a Antibody validated in Immunoprecipitation " width="137" height="214" caption="false" /></p>
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<div class="small-9 columns">
<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 μg of the Diagenode antibody against JMJD2a (Cat. No. C15410332, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JMJD2a protein was detected by western blot with the JMJD2a antibody diluted 1:200. </small></p>
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<small>Manufactured by Bethyl Laboratories, Inc., Texas, USA</small></div>',
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><img src="https://www.diagenode.com/img/product/antibodies/c15410332-chip.png" alt="JMJD2a Antibody ChIP Grade" caption="false" width="288" height="218" /></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-a.jpg" alt="JMJD2a Antibody ChIP-seq Grade" caption="false" width="922" height="109" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-b.jpg" alt="JMJD2a Antibody for ChIP-seq assay" caption="false" width="922" height="181" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-c.jpg" alt="JMJD2a Antibody validated in ChIP-seq" caption="false" width="922" height="139" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-d.jpg" alt="JMJD2a Antibody for ChIP-seq" caption="false" width="922" height="122" /></div>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<div class="small-9 columns">
<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Whole cell extracts from HeLa (lane 1) and mouse NIH3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. C15410332) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 μg of the Diagenode antibody against JMJD2a (Cat. No. C15410332, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JMJD2a protein was detected by western blot with the JMJD2a antibody diluted 1:200. </small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Whole cell extracts from HeLa (lane 1) and mouse NIH3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. C15410332) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 μg of the Diagenode antibody against JMJD2a (Cat. No. C15410332, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JMJD2a protein was detected by western blot with the JMJD2a antibody diluted 1:200. </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Whole cell extracts from HeLa (lane 1) and mouse NIH3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. C15410332) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 μg of the Diagenode antibody against JMJD2a (Cat. No. C15410332, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JMJD2a protein was detected by western blot with the JMJD2a antibody diluted 1:200. </small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<div class="small-12 columns">A.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-a.jpg" alt="JMJD2a Antibody ChIP-seq Grade" caption="false" width="922" height="109" /><br /> B.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-b.jpg" alt="JMJD2a Antibody for ChIP-seq assay" caption="false" width="922" height="181" /><br /> C.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-c.jpg" alt="JMJD2a Antibody validated in ChIP-seq" caption="false" width="922" height="139" /><br /> D.<img src="https://www.diagenode.com/img/product/antibodies/c15410332-chipseq-d.jpg" alt="JMJD2a Antibody for ChIP-seq" caption="false" width="922" height="122" /></div>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Whole cell extracts from HeLa (lane 1) and mouse NIH3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. C15410332) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 μg of the Diagenode antibody against JMJD2a (Cat. No. C15410332, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JMJD2a protein was detected by western blot with the JMJD2a antibody diluted 1:200. </small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Whole cell extracts from HeLa (lane 1) and mouse NIH3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. C15410332) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 μg of the Diagenode antibody against JMJD2a (Cat. No. C15410332, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JMJD2a protein was detected by western blot with the JMJD2a antibody diluted 1:200. </small></p>
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<p><small><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). </small></p>
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<p><small><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against JMJD2a</strong><br /> ChIP assays were performed using K562 cells, the Diagenode antibody against JMJD2a (Cat. No. C15410332) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the promoters of the c-fos and FBXL18 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</small></p>
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<p><small><strong>Figure 3. Western blot analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Whole cell extracts from HeLa (lane 1) and mouse NIH3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against JMJD2a (Cat. No. C15410332) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. </small></p>
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<div class="small-9 columns">
<p><small><strong>Figure 4. Immunoprecipitation analysis using the Diagenode antibody directed against JMJD2a</strong><br /> Immunoprecipitation was performed on whole cell extracts from HeLa cells using 3 μg of the Diagenode antibody against JMJD2a (Cat. No. C15410332, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated JMJD2a protein was detected by western blot with the JMJD2a antibody diluted 1:200. </small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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